AT2G19930
ERF Family

Probably involved in the RNA silencing pathway and required for the generation of small interfering RNAs (siRNAs)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0000505
Physical Location & Seq
Reverse (-)
8607340 .. 8612648
5309 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G19930.1

Sequence Viewer

Length: 2934 bp
ATGAATCAAGCTTCTTCGAGGATAAGGATCGCTCTTTCGGGTCGGATTGAAACAGCTTTAGAGAATATCTACAGAAAACACAATCTTACTCCGATTAATGATGAGACGAGACAGAGACTGTCTTCGATTCCTGAGAATTTGGGTTTTGAGTTGGTCAGGAAAGTTTTTAGTTTGCAGGCTGGTTTGATATACAACCTCGACAGCTTCATCGTTTCTAAGGTTAACCAAGCCGTTAGTTTTACTGGCTATCCACAGCCGGCGAACTCTCTTAGCCCCTCTGGAAGACACGTGTCCAGGGTTCTTCAAGAAGAGATGTCTGTTGATTCTGATGCTCCTTCTCCTAAGTCTTTGAAAAGTGAAGACAAAGGAGGATCTCTGCACATTCCCCAGCTGGTAGCTTTGGGTGAACTTGAATTTAAGAAGGTGTTTTTGTTACTTAGCTATATTCCAGGGCAACATGTGGGCCAGGTTATAACTGCTGATGAGATTAGACTGTGGAAAGATTTGCCAATGGTTGAATATGAAGCAGCGGTTTGGGACCGCTTGGGCCGGCATTATTGTCCCCAAAAGGACCGAAGAATGTTGCAATGGGATAGCGGGAAGACCCATTACTATCAGTGTAATGTTGCTCCCAATGGTAGCTACACATTCAAGGTTCTTTCAGCTCTTCAAGGCCCTCTTCTTGAACACACTGGAACACACTTGCACAAGGTCTTGGGTGATGATAATGTTTTAACCGTCAAATTCGCGGATGTCCAGAAAAGTTCATCAACATATAGCATTGACCATTACTTTACATACAAAGGGATTGCCAAGAACGGTATCATGATTGGTTTACGTCGTTATCAATTTTTTGTTTTCAAAGATGGAGGAAAAGAGGAAAAGAAGAAAGATCTTTCTACAAAGAAAGTGAAATGCTACTTTATACGCACGGACTCAACAGCTTTTTATGATATGCAAAATCCCTATATTTTAACCGGAAAGTCAATTTATGAAGCTCGTATGCATTTTATGCACGTGCATCGAGCACCAACTTTGGCGAATTATATGGCAAGGTTTTCCTTAATTCTGTCAAAGACTAAAACGCTTGAAGTTGACATGACTGGGATTACCTTTGATCAAATTGATGATATACACTGCCATGATCAAGATGGGAAAGATGTTCTTGACAAGAACAAAAAACCTTGCATACATTCAGATGGAACTGGCTACATCTCTGAGGACCTGGCTCGGATGTGTCCCCTAAATATATTTAAAGGGAAATGTCTCAGAAGTGAAAGTATTCAGGAAGCATGTTACCAAGACCCGCCTCTTTTGATCCAGTTTCGGATGTTTTATGATGGTTATGCTGTTAAGGGAACTTTTCTCTTAAACAAGAAACTTTGTCCTCGGACTGTCCAGGTTCGACCTTCGATGATCAAGGTGTCTAAAGATCCATCCTTGTCCAATTTTAGCACCTTTAACGCCTTGGAAGTAGTAACCACAAGTAATCCACCAAAAAGAACAAAGCTATCAAAAAATTTGGTTGCGCTGCTTAGCTATGGAGGAATCCCTAATGAATTCTTTTTGGATATATTGCTCAACACGTTGGAAGAGTCTAAAAGCATATTCTACAACAAACGTGCTGCACTTAATGCTGCTCTTAATTATGGGGAAATGGACGACCAGAACGCCGCACAGATGATATTGGTTGGTATCCCCCTTGATGAACCACACTTGAAGAATTATCTGTCTATACTTTTAAAGACAGAGAAAAATGATCTCAAAGCAGGAAAACTTCCTGTGACCGAATCATACTATCTTATGGGAACAGTGGATCCCACCGGAGCGCTAAAGGAAGATGAAGTCTGTGTAATTCTTGAGTCTGGGCAAATTTCGGGAGAAGTTTTAGTGTATAGAAATCCTGGATTACATTTTGGAGACATACATATTCTTAAGGCCACATATGTCAAGGCCTTGGAAGAGTATGTTGGAAATTCCAAGTTTGCTGTGTTCTTCCCTCAGAAAGGTCCAAGATCCTTGGGGGACGAGATTGCAGGTGGTGACTTTGATGGTGATATGTATTTCATATCAAGAAACCCTGAGCTACTTGAAAACTTCAAACCAAGTGAACCGTGGGTGAGTTTGACTCCTCCCAGTAAAAGTAACTCTGGTAGAGCGCCAAGCCAGCTTTCCCCAGAAGAGTTGGAAGAAGAACTTTTCGAAATGTTCCTGACTGCAGGATTTCATGCCAGCAATGTTATAGGGATAGCTGCAGATAGCTGGTTAACAATAATGGACCGCTTCCTCATACTAGGAGATGACAGGGCTGAGGAAAAAGCTGAAATGAAGAAGAAGATGCTTGAGCTGATTGATATATACTATGATGCTCTTGATGCACCAAAGAAAGGGGATAAAGTCTACCTCCCAAACAAGCTGAAGCCTGACATCTTTCCACATTACATGGAGCGGGATAAAAAATTCCAGTCCACTTCTATTCTTGGATTAATCTTTGACTTTGTTAAATCACAGACAACAGAGGAACCCTCACCATCATCTGAGATCAGTAAACTCCCGTGTTTCGAAGATGAGCCGGTCTCCGAGTTTCATATGCAGAAATGTAGACTGTGGTATGATAATTACAGAACTGAGATGACACAGGCGATGAAGACTGATAAGGATGAATCAGCCAATGAAGTTATTCAGAGATACAAGCAGGAGTTCTATGGCGCTGCAGGGTTTGAAGACAGCAAGAAAAGTCTGGAAGAACTCTATCCGCAAGCTCTCGCACTCTACAAGATCGTTTACGATTATGCCATTCATGCAGGTGTTTCGAAATGCAGGTTTGTCTGGAAGGTAGCAGGACCGGTACTGTGCAGATTCTATCTAAATAAGAAAATGCAGGAGAAATGTTTAGTGTGTGCACCATCTGTGCTTAAAGAGCTTTGGGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RdRP

Protein Analysis

977

Amino Acids

110.89

Weight (kDa)

6.67

Isoelectric Point (pI)

44.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
4HB_RdRP3_N PF26249 8 - 84 1.9e-32 RdRP3/4/5, N-terminal four helical bundle domain
RdRP_helical PF26252 129 - 197 1.1e-17 RNA dependent RNA polymerase, helical domain
RdRP PF05183 222 - 832 6.1e-109 RNA dependent RNA polymerase
RdRP_head PF26253 852 - 971 4.5e-26 RdRP, head domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000505)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G19910 AT2G19910 AT2G19920 AT2G19920 AT2G19920 AT2G19920 AT2G19930 AT2G19930 AT2G19930
fragaria_vesca FvH4_1g05980 FvH4_1g05990 FvH4_1g05990 FvH4_1g09718
malus_domestica MD02G1064400.v1.1 MD15G1195300.v1.1 MD15G1195400.v1.1
prunus_persica Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1
pyrus_communis pycom02g05070 pycom15g17460
rosa_chinensis RchiOBHm_Chr2g0091671 RchiOBHm_Chr2g0091681 RchiOBHm_Chr2g0091691 RchiOBHm_Chr2g0091701 RchiOBHm_Chr2g0091711 RchiOBHm_Chr2g0091721 RchiOBHm_Chr2g0091731 RchiOBHm_Chr5g0014401
rosa_laevigata RLG00000013772 RLG00000016242 RLG00000016244 RLG00000016245 RLG00000016246 RLG00000032105
rosa_multiflora Rmu_co8374041.1_g000001 Rmu_co8478121.1_g000001 Rmu_sc0000897.1_g000005 Rmu_sc0000897.1_g000009
rosa_roxburghii Rroxscaffold_1G00062180 Rroxscaffold_1G00062190 Rroxscaffold_1G00068800 Rroxscaffold_2G00149790 Rroxscaffold_2G00149800 Rroxscaffold_2G00149810
rosa_rugosa Rorug02G0019600 Rorug02G0019700 Rorug02G0019800 Rorug02G0019900 Rorug02G0019900 Rorug02G0020000 Rorug02G0020000 Rorug02G0020100 Rorug02G0020100 Rorug02G0020200 Rorug05G0017800
rosa_samantha Rh2AG064700 Rh2AG064800 Rh2AG064900 Rh2BG063800 Rh2BG063900 Rh2BG064000 Rh2BG064100 Rh2CG065500 Rh2CG065600 Rh2CG065700 Rh2DG063900 Rh2DG064000 Rh2DG064100 Rh2DG064200 Rh2DG381700 Rh5AG056700 Rh5AG056800 Rh5AG056900 Rh5AG112600 Rh5BG109300 Rh5CG063800 Rh5CG121100 Rh5DG053100 Rh5DG108300 Rh6CG100100
rosa_wichuraiana Rw2G005560 Rw2G005580 Rw2G005590 Rw5G005370 Rw5G009770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 473
AarI CACCTGC 2 cut(s) 2027, 2795
Acc36I ACCTGC 3 cut(s) 2027, 2795, 2811
AccBSI CCGCTC 1 cut(s) 2449
AccI GTMKAC 2 cut(s) 2400, 2602
AccII CGCG 1 cut(s) 749
AciI CCGC 9 cut(s) 530, 541, 597, 749, 1307, 1674, 2281, 2449, 2756
AclWI GGATC 7 cut(s) 35, 379, 1312, 1427, 1811, 1824, 2010
AcsI RAATTY 8 cut(s) 136, 413, 743, 1519, 1559, 1872, 1975, 2459
AcuI CTGAAG 1 cut(s) 2438
AcvI CACGTG 2 cut(s) 289, 1018
AfaI GTAC 1 cut(s) 2850
AfeI AGCGCT 1 cut(s) 1830
AfiI CCNNNNNNNGG 2 cut(s) 2006, 2387
AflII CTTAAG 1 cut(s) 1934
AflIII ACRYGT 4 cut(s) 286, 288, 457, 1584
AgeI ACCGGT 1 cut(s) 2845
AjnI CCWGG 6 cut(s) 293, 448, 465, 1224, 1398, 1903
AjuI GAANNNNNNNTTGG 4 cut(s) 1953, 1985, 2155, 2187
AloI GAACNNNNNNTCC 2 cut(s) 1801, 1833
Alw21I GWGCWC 2 cut(s) 1030, 2905
Alw26I GTCTC 6 cut(s) 98, 103, 109, 1271, 1914, 2581
Alw44I GTGCAC 1 cut(s) 2901
AlwI GGATC 7 cut(s) 35, 379, 1312, 1427, 1811, 1824, 2010
AlwNI CAGNNNCTG 1 cut(s) 118
Aor51HI AGCGCT 1 cut(s) 1830
AoxI GGCC 5 cut(s) 463, 547, 673, 1938, 1953
ApaLI GTGCAC 1 cut(s) 2901
ApeKI GCWGC 6 cut(s) 527, 1531, 1625, 1637, 2252, 2711
ApoI RAATTY 8 cut(s) 136, 413, 743, 1519, 1559, 1872, 1975, 2459
AseI ATTAAT 2 cut(s) 96, 2486
AsiGI ACCGGT 1 cut(s) 2845
Asp700I GAANNNNTTC 2 cut(s) 1281, 2679
AspLEI GCGC 4 cut(s) 1531, 1831, 2161, 2711
AspS9I GGNCC 9 cut(s) 463, 538, 547, 571, 674, 1222, 2009, 2278, 2843
AsuHPI GGTGA 6 cut(s) 416, 731, 2054, 2066, 2131, 2520
AsuII TTCGAA 3 cut(s) 2202, 2562, 2813
AvaII GGWCC 6 cut(s) 538, 571, 1222, 2009, 2278, 2843
BaeGI GKGCMC 1 cut(s) 2905
BamHI GGATCC 1 cut(s) 1816
BarI GAAGNNNNNNTAC 2 cut(s) 593, 625
BbrPI CACGTG 2 cut(s) 289, 1018
BbsI GAAGAC 6 cut(s) 114, 289, 366, 608, 2654, 2730
Bbv12I GWGCWC 2 cut(s) 1030, 2905
BbvCI CCTCAGC 1 cut(s) 2310
BbvI GCAGC 6 cut(s) 539, 1518, 1612, 1624, 2239, 2698
BccI CCATC 8 cut(s) 860, 1145, 1193, 1334, 1444, 2045, 2539, 2914
BceAI ACGGC 1 cut(s) 215
BcgI CGANNNNNNTGC 4 cut(s) 1004, 1038, 2793, 2827
BciT130I CCWGG 6 cut(s) 295, 450, 467, 1226, 1400, 1905
BciVI GTATCC 1 cut(s) 1706
BclI TGATCA 3 cut(s) 1117, 1144, 1416
BcoDI GTCTC 6 cut(s) 98, 103, 109, 1271, 1914, 2581
BfaI CTAG 1 cut(s) 2294
BfmI CTRYAG 4 cut(s) 70, 2217, 2253, 2712
BfoI RGCGCY 3 cut(s) 1832, 2162, 2712
BfrI CTTAAG 1 cut(s) 1934
BfuAI ACCTGC 3 cut(s) 2027, 2795, 2811
BfuI GTATCC 1 cut(s) 1706
BglII AGATCT 1 cut(s) 892
BisI GCNGC 7 cut(s) 528, 1532, 1626, 1638, 1674, 2253, 2712
BlpI GCTNAGC 1 cut(s) 1535
BlsI GCNGC 7 cut(s) 529, 1533, 1627, 1639, 1675, 2254, 2713
Bme1390I CCNGG 6 cut(s) 295, 450, 467, 1226, 1400, 1905
Bme18I GGWCC 6 cut(s) 538, 571, 1222, 2009, 2278, 2843
BmgT120I GGNCC 9 cut(s) 463, 538, 547, 571, 674, 1222, 2009, 2278, 2843
BmiI GGNNCC 3 cut(s) 539, 1818, 2523
BmrFI CCNGG 6 cut(s) 295, 450, 467, 1226, 1400, 1905
BmrI ACTGGG 2 cut(s) 1113, 2130
BmsI GCATC 5 cut(s) 319, 1030, 2328, 2356, 2365
BmuI ACTGGG 2 cut(s) 1113, 2130
BoxI GACNNNNGTC 1 cut(s) 289
BpiI GAAGAC 6 cut(s) 114, 289, 366, 608, 2654, 2730
BplI GAGNNNNNCTC 6 cut(s) 2113, 2145, 2510, 2542, 2561, 2593
Bpu10I CCTNAGC 2 cut(s) 2082, 2310
Bpu1102I GCTNAGC 1 cut(s) 1535
Bpu14I TTCGAA 3 cut(s) 2202, 2562, 2813
BpuEI CTTGAG 2 cut(s) 1880, 2363
BsaAI YACGTR 2 cut(s) 289, 1018
BsaBI GATNNNNATC 1 cut(s) 26
BsaI GGTCTC 1 cut(s) 2581
BsaJI CCNNGG 7 cut(s) 294, 449, 1388, 1467, 1956, 2019, 2114
BsaWI WCCGGW 3 cut(s) 977, 1823, 2845
Bsc4I CCNNNNNNNGG 2 cut(s) 2006, 2387
Bse118I RCCGGY 4 cut(s) 256, 549, 2572, 2845
Bse1I ACTGG 7 cut(s) 247, 697, 1108, 1210, 1321, 2136, 2464
Bse3DI GCAATG 2 cut(s) 593, 2242
Bse8I GATNNNNATC 1 cut(s) 26
BseBI CCWGG 6 cut(s) 295, 450, 467, 1226, 1400, 1905
BseDI CCNNGG 7 cut(s) 294, 449, 1388, 1467, 1956, 2019, 2114
BseGI GGATG 5 cut(s) 757, 1239, 1335, 1436, 2665
BseJI GATNNNNATC 1 cut(s) 26
BseLI CCNNNNNNNGG 2 cut(s) 2006, 2387
BseMI GCAATG 2 cut(s) 593, 2242
BseMII CTCAG 8 cut(s) 123, 1209, 1282, 2015, 2073, 2301, 2529, 2619
BseNI ACTGG 7 cut(s) 247, 697, 1108, 1210, 1321, 2136, 2464
BseRI GAGGAG 1 cut(s) 2121
BseSI GKGCMC 1 cut(s) 2905
BseXI GCAGC 6 cut(s) 539, 1518, 1612, 1624, 2239, 2698
BseYI CCCAGC 1 cut(s) 387
BsgI GTGCAG 3 cut(s) 362, 1611, 2875
Bsh1236I CGCG 1 cut(s) 749
BshFI GGCC 5 cut(s) 465, 549, 675, 1940, 1955
BshTI ACCGGT 1 cut(s) 2845
BsiHKAI GWGCWC 2 cut(s) 1030, 2905
BsiSI CCGG 6 cut(s) 257, 550, 978, 1824, 2573, 2846
BslFI GGGAC 4 cut(s) 546, 551, 1224, 2039
BslI CCNNNNNNNGG 2 cut(s) 2006, 2387
BsmAI GTCTC 6 cut(s) 98, 103, 109, 1271, 1914, 2581
BsmBI CGTCTC 1 cut(s) 98
BsmFI GGGAC 4 cut(s) 546, 551, 1224, 2039
BsnI GGCC 5 cut(s) 465, 549, 675, 1940, 1955
Bso31I GGTCTC 1 cut(s) 2581
Bsp119I TTCGAA 3 cut(s) 2202, 2562, 2813
Bsp1286I GDGCHC 2 cut(s) 1030, 2905
Bsp1720I GCTNAGC 1 cut(s) 1535
BspACI CCGC 9 cut(s) 530, 541, 597, 749, 1307, 1674, 2281, 2449, 2756
BspANI GGCC 5 cut(s) 465, 549, 675, 1940, 1955
BspCNI CTCAG 8 cut(s) 124, 1210, 1281, 2014, 2074, 2302, 2530, 2620
BspFNI CGCG 1 cut(s) 749
BspHI TCATGA 1 cut(s) 825
BspLI GGNNCC 3 cut(s) 539, 1818, 2523
BspMAI CTGCAG 3 cut(s) 2221, 2257, 2716
BspMI ACCTGC 3 cut(s) 2027, 2795, 2811
BspPI GGATC 7 cut(s) 35, 379, 1312, 1427, 1811, 1824, 2010
BspQI GCTCTTC 1 cut(s) 672
BspT104I TTCGAA 3 cut(s) 2202, 2562, 2813
BspTI CTTAAG 1 cut(s) 1934
BspTNI GGTCTC 1 cut(s) 2581
BsrBI CCGCTC 1 cut(s) 2449
BsrDI GCAATG 2 cut(s) 593, 2242
BsrFI RCCGGY 4 cut(s) 256, 549, 2572, 2845
BsrI ACTGG 7 cut(s) 247, 697, 1108, 1210, 1321, 2136, 2464
BssAI RCCGGY 4 cut(s) 256, 549, 2572, 2845
BssECI CCNNGG 7 cut(s) 294, 449, 1388, 1467, 1956, 2019, 2114
BssT1I CCWWGG 3 cut(s) 1467, 1956, 2019
Bst2UI CCWGG 6 cut(s) 295, 450, 467, 1226, 1400, 1905
Bst4CI ACNGT 9 cut(s) 120, 495, 739, 821, 1396, 1813, 2115, 2607, 2853
Bst6I CTCTTC 6 cut(s) 303, 672, 684, 1587, 1956, 2175
BstAFI CTTAAG 1 cut(s) 1934
BstAPI GCANNNNNTGC 2 cut(s) 1012, 1634
BstBAI YACGTR 2 cut(s) 289, 1018
BstBI TTCGAA 3 cut(s) 2202, 2562, 2813
BstC8I GCNNGC 6 cut(s) 177, 258, 551, 2168, 2233, 2760
BstDSI CCRYGG 1 cut(s) 2114
BstENI CCTNNNNNAGG 1 cut(s) 2004
BstF5I GGATG 5 cut(s) 757, 1239, 1335, 1436, 2665
BstFNI CGCG 1 cut(s) 749
BstH2I RGCGCY 3 cut(s) 1832, 2162, 2712
BstHHI GCGC 4 cut(s) 1531, 1831, 2161, 2711
BstMAI GTCTC 6 cut(s) 98, 103, 109, 1271, 1914, 2581
BstMWI GCNNNNNNNGC 6 cut(s) 1012, 1634, 2167, 2375, 2801, 2920
BstNI CCWGG 6 cut(s) 295, 450, 467, 1226, 1400, 1905
BstNSI RCATGY 2 cut(s) 461, 1296
BstPAI GACNNNNGTC 1 cut(s) 289
BstSCI CCNGG 6 cut(s) 293, 448, 465, 1224, 1398, 1903
BstSFI CTRYAG 4 cut(s) 70, 2217, 2253, 2712
BstSLI GKGCMC 1 cut(s) 2905
BstUI CGCG 1 cut(s) 749
BstV1I GCAGC 6 cut(s) 539, 1518, 1612, 1624, 2239, 2698
BstV2I GAAGAC 6 cut(s) 114, 289, 366, 608, 2654, 2730
BstX2I RGATCY 5 cut(s) 371, 892, 1432, 1816, 2015
BstYI RGATCY 5 cut(s) 371, 892, 1432, 1816, 2015
BsuI GTATCC 1 cut(s) 1706
BsuRI GGCC 5 cut(s) 465, 549, 675, 1940, 1955
BtgI CCRYGG 1 cut(s) 2114
BtgZI GCGATG 1 cut(s) 2657
BtsCI GGATG 5 cut(s) 757, 1239, 1335, 1436, 2665
BtsI GCAGTG 1 cut(s) 1135
BtsIMutI CAGTG 4 cut(s) 623, 690, 1135, 1818
BveI ACCTGC 3 cut(s) 2027, 2795, 2811
Cac8I GCNNGC 6 cut(s) 177, 258, 551, 2168, 2233, 2760
CaiI CAGNNNCTG 1 cut(s) 118
CciI TCATGA 1 cut(s) 825
CfoI GCGC 4 cut(s) 1531, 1831, 2161, 2711
Cfr10I RCCGGY 4 cut(s) 256, 549, 2572, 2845
Cfr13I GGNCC 9 cut(s) 463, 538, 547, 571, 674, 1222, 2009, 2278, 2843
Csp6I GTAC 1 cut(s) 2849
CspAI ACCGGT 1 cut(s) 2845
CviAII CATG 8 cut(s) 458, 826, 1099, 1142, 1293, 2228, 2443, 2801
CviQI GTAC 1 cut(s) 2849
DraI TTTAAA 2 cut(s) 1255, 1743
Eam1104I CTCTTC 6 cut(s) 303, 672, 684, 1587, 1956, 2175
EarI CTCTTC 6 cut(s) 303, 672, 684, 1587, 1956, 2175
Eco130I CCWWGG 3 cut(s) 1467, 1956, 2019
Eco147I AGGCCT 1 cut(s) 1955
Eco31I GGTCTC 1 cut(s) 2581
Eco47I GGWCC 6 cut(s) 538, 571, 1222, 2009, 2278, 2843
Eco47III AGCGCT 1 cut(s) 1830
Eco57I CTGAAG 1 cut(s) 2438
Eco72I CACGTG 2 cut(s) 289, 1018
EcoNI CCTNNNNNAGG 1 cut(s) 2004
EcoO109I RGGNCCY 2 cut(s) 674, 1222
EcoRI GAATTC 1 cut(s) 1559
EcoRII CCWGG 6 cut(s) 293, 448, 465, 1224, 1398, 1903
EcoT14I CCWWGG 3 cut(s) 1467, 1956, 2019
EcoT22I ATGCAT 1 cut(s) 1008
ErhI CCWWGG 3 cut(s) 1467, 1956, 2019
Esp3I CGTCTC 1 cut(s) 98
FaeI CATG 8 cut(s) 461, 829, 1102, 1145, 1296, 2231, 2446, 2804
FalI AAGNNNNNCTT 6 cut(s) 663, 695, 1149, 1181, 2181, 2213
FaqI GGGAC 4 cut(s) 546, 551, 1224, 2039
FatI CATG 8 cut(s) 457, 825, 1098, 1141, 1292, 2227, 2442, 2800
FauI CCCGC 3 cut(s) 590, 1314, 2442
FauNDI CATATG 2 cut(s) 1945, 2589
FbaI TGATCA 3 cut(s) 1117, 1144, 1416
FblI GTMKAC 2 cut(s) 2400, 2602
Fnu4HI GCNGC 7 cut(s) 528, 1532, 1626, 1638, 1674, 2253, 2712
FokI GGATG 5 cut(s) 764, 1246, 1342, 1423, 2672
Fsp4HI GCNGC 7 cut(s) 528, 1532, 1626, 1638, 1674, 2253, 2712
FspBI CTAG 1 cut(s) 2294
GlaI GCGC 4 cut(s) 1530, 1830, 2160, 2710
GluI GCNGC 7 cut(s) 528, 1532, 1626, 1638, 1674, 2253, 2712
GsaI CCCAGC 1 cut(s) 391
HaeII RGCGCY 3 cut(s) 1832, 2162, 2712
HaeIII GGCC 5 cut(s) 465, 549, 675, 1940, 1955
HapII CCGG 6 cut(s) 257, 550, 978, 1824, 2573, 2846
HhaI GCGC 4 cut(s) 1531, 1831, 2161, 2711
Hin1II CATG 8 cut(s) 461, 829, 1102, 1145, 1296, 2231, 2446, 2804
Hin6I GCGC 4 cut(s) 1529, 1829, 2159, 2709
HinP1I GCGC 4 cut(s) 1529, 1829, 2159, 2709
HincII GTYRAC 3 cut(s) 223, 1096, 2268
HindII GTYRAC 3 cut(s) 223, 1096, 2268
HindIII AAGCTT 1 cut(s) 9
HpaI GTTAAC 2 cut(s) 223, 2268
HpaII CCGG 6 cut(s) 257, 550, 978, 1824, 2573, 2846
HphI GGTGA 6 cut(s) 416, 731, 2054, 2066, 2131, 2520
Hpy99I CGWCG 1 cut(s) 843
HpyAV CCTTC 4 cut(s) 345, 415, 1419, 2827
HpyCH4III ACNGT 9 cut(s) 120, 495, 739, 821, 1396, 1813, 2115, 2607, 2853
HpyCH4IV ACGT 5 cut(s) 288, 838, 1017, 1586, 1621
HpyF10VI GCNNNNNNNGC 6 cut(s) 1012, 1634, 2167, 2375, 2801, 2920
HpySE526I ACGT 5 cut(s) 288, 838, 1017, 1586, 1621
Hsp92II CATG 8 cut(s) 461, 829, 1102, 1145, 1296, 2231, 2446, 2804
HspAI GCGC 4 cut(s) 1529, 1829, 2159, 2709
KroI GCCGGC 2 cut(s) 256, 549
KroNI GCCGGC 2 cut(s) 258, 551
Ksp22I TGATCA 3 cut(s) 1117, 1144, 1416
KspAI GTTAAC 2 cut(s) 223, 2268
LguI GCTCTTC 1 cut(s) 672
LmnI GCTCC 4 cut(s) 337, 634, 1826, 2446
Lsp1109I GCAGC 6 cut(s) 539, 1518, 1612, 1624, 2239, 2698
LweI GCATC 5 cut(s) 319, 1030, 2328, 2356, 2365
MaeI CTAG 1 cut(s) 2294
MaeII ACGT 5 cut(s) 288, 838, 1017, 1586, 1621
MaeIII GTNAC 6 cut(s) 432, 1295, 1477, 1783, 2042, 2144
MbiI CCGCTC 1 cut(s) 2449
MflI RGATCY 5 cut(s) 371, 892, 1432, 1816, 2015
MhlI GDGCHC 2 cut(s) 1030, 2905
MlyI GAGTC 4 cut(s) 929, 1604, 1871, 2122
MmeI TCCRAC 4 cut(s) 23, 1569, 1951, 2166
Mph1103I ATGCAT 1 cut(s) 1008
MroNI GCCGGC 2 cut(s) 256, 549
MroXI GAANNNNTTC 2 cut(s) 1281, 2679
MslI CAYNNNNRTG 3 cut(s) 1140, 1197, 2805
MspA1I CMGCKG 2 cut(s) 391, 530
MspCI CTTAAG 1 cut(s) 1934
MspI CCGG 6 cut(s) 257, 550, 978, 1824, 2573, 2846
MspR9I CCNGG 6 cut(s) 295, 450, 467, 1226, 1400, 1905
MvaI CCWGG 6 cut(s) 295, 450, 467, 1226, 1400, 1905
MvnI CGCG 1 cut(s) 749
MwoI GCNNNNNNNGC 6 cut(s) 1012, 1634, 2167, 2375, 2801, 2920
NaeI GCCGGC 2 cut(s) 258, 551
NdeI CATATG 2 cut(s) 1945, 2589
NgoMIV GCCGGC 2 cut(s) 256, 549
NlaIII CATG 8 cut(s) 461, 829, 1102, 1145, 1296, 2231, 2446, 2804
NlaIV GGNNCC 3 cut(s) 539, 1818, 2523
NmuCI GTSAC 2 cut(s) 1783, 2042
NsiI ATGCAT 1 cut(s) 1008
NspI RCATGY 2 cut(s) 461, 1296
NspV TTCGAA 3 cut(s) 2202, 2562, 2813
PagI TCATGA 1 cut(s) 825
PaqCI CACCTGC 2 cut(s) 2027, 2795
PceI AGGCCT 1 cut(s) 1955
PciI ACATGT 1 cut(s) 457
PciSI GCTCTTC 1 cut(s) 672
PdiI GCCGGC 2 cut(s) 258, 551
PdmI GAANNNNTTC 2 cut(s) 1281, 2679
PfeI GAWTC 7 cut(s) 4, 127, 323, 1548, 1790, 2663, 2859
PfoI TCCNGGA 1 cut(s) 1903
PinAI ACCGGT 1 cut(s) 2845
PkrI GCNGC 7 cut(s) 529, 1533, 1627, 1639, 1675, 2254, 2713
PleI GAGTC 4 cut(s) 929, 1603, 1870, 2122
PmaCI CACGTG 2 cut(s) 289, 1018
PmlI CACGTG 2 cut(s) 289, 1018
PpsI GAGTC 4 cut(s) 929, 1603, 1870, 2122
Ppu21I YACGTR 2 cut(s) 289, 1018
PpuMI RGGWCCY 1 cut(s) 1222
PscI ACATGT 1 cut(s) 457
PshAI GACNNNNGTC 1 cut(s) 289
PshBI ATTAAT 2 cut(s) 96, 2486
PsiI TTATAA 1 cut(s) 473
Psp5II RGGWCCY 1 cut(s) 1222
Psp6I CCWGG 6 cut(s) 293, 448, 465, 1224, 1398, 1903
PspCI CACGTG 2 cut(s) 289, 1018
PspFI CCCAGC 1 cut(s) 387
PspGI CCWGG 6 cut(s) 293, 448, 465, 1224, 1398, 1903
PspN4I GGNNCC 3 cut(s) 539, 1818, 2523
PspPI GGNCC 9 cut(s) 463, 538, 547, 571, 674, 1222, 2009, 2278, 2843
PspPPI RGGWCCY 1 cut(s) 1222
PstI CTGCAG 3 cut(s) 2221, 2257, 2716
PstNI CAGNNNCTG 1 cut(s) 118
PsuI RGATCY 5 cut(s) 371, 892, 1432, 1816, 2015
PvuII CAGCTG 1 cut(s) 391
RsaI GTAC 1 cut(s) 2850
RsaNI GTAC 1 cut(s) 2849
RseI CAYNNNNRTG 3 cut(s) 1140, 1197, 2805
SapI GCTCTTC 1 cut(s) 672
SatI GCNGC 7 cut(s) 528, 1532, 1626, 1638, 1674, 2253, 2712
Sau96I GGNCC 9 cut(s) 463, 538, 547, 571, 674, 1222, 2009, 2278, 2843
SchI GAGTC 4 cut(s) 929, 1604, 1871, 2122
ScrFI CCNGG 6 cut(s) 295, 450, 467, 1226, 1400, 1905
SduI GDGCHC 2 cut(s) 1030, 2905
SfaNI GCATC 5 cut(s) 319, 1030, 2328, 2356, 2365
SfcI CTRYAG 4 cut(s) 70, 2217, 2253, 2712
SfuI TTCGAA 3 cut(s) 2202, 2562, 2813
SinI GGWCC 6 cut(s) 538, 571, 1222, 2009, 2278, 2843
SmiMI CAYNNNNRTG 3 cut(s) 1140, 1197, 2805
SmlI CTYRAG 3 cut(s) 1859, 1934, 2342
SmoI CTYRAG 3 cut(s) 1859, 1934, 2342
SseBI AGGCCT 1 cut(s) 1955
SsiI CCGC 9 cut(s) 530, 541, 597, 749, 1307, 1674, 2281, 2449, 2756
SspMI CTAG 1 cut(s) 2294
StuI AGGCCT 1 cut(s) 1955
StyD4I CCNGG 6 cut(s) 293, 448, 465, 1224, 1398, 1903
StyI CCWWGG 3 cut(s) 1467, 1956, 2019
TaaI ACNGT 9 cut(s) 120, 495, 739, 821, 1396, 1813, 2115, 2607, 2853
TaiI ACGT 5 cut(s) 291, 841, 1020, 1589, 1624
TaqI TCGA 9 cut(s) 17, 125, 198, 1024, 1405, 1412, 2202, 2562, 2813
TaqII GACCGA 2 cut(s) 588, 1802
TauI GCSGC 1 cut(s) 1676
TfiI GAWTC 7 cut(s) 4, 127, 323, 1548, 1790, 2663, 2859
TscAI CASTG 4 cut(s) 623, 697, 1142, 1818
TseFI GTSAC 2 cut(s) 1783, 2042
TseI GCWGC 6 cut(s) 527, 1531, 1625, 1637, 2252, 2711
Tsp45I GTSAC 2 cut(s) 1783, 2042
TspGWI ACGGA 1 cut(s) 947
TspRI CASTG 4 cut(s) 623, 697, 1142, 1818
Vha464I CTTAAG 1 cut(s) 1934
VneI GTGCAC 1 cut(s) 2901
VpaK11BI GGWCC 6 cut(s) 538, 571, 1222, 2009, 2278, 2843
VspI ATTAAT 2 cut(s) 96, 2486
XagI CCTNNNNNAGG 1 cut(s) 2004
XapI RAATTY 8 cut(s) 136, 413, 743, 1519, 1559, 1872, 1975, 2459
XceI RCATGY 2 cut(s) 461, 1296
XcmI CCANNNNNNNNNTGG 2 cut(s) 1148, 2112
XmiI GTMKAC 2 cut(s) 2400, 2602
XmnI GAANNNNTTC 2 cut(s) 1281, 2679
XspI CTAG 1 cut(s) 2294
Zsp2I ATGCAT 1 cut(s) 1008
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.