Rroxscaffold_1G00068800
ERF Family

Probably involved in the RNA silencing pathway and required for the generation of small interfering RNAs (siRNAs)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000505
Physical Location & Seq
Reverse (-)
89864417 .. 89875460
11044 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00068800.1

Sequence Viewer

Length: 3105 bp
ATGATGAAGCAGAGTTGGGATGAGCAAGAAGAGGTGCCGCGTCTACCTCAGAAAGTGGAGGCATTGCTCCGGCAAATCTGTGAGGACAAAAAGCAACCTTGGCCTGACGCTGACGCCATAAGGCAGCTCGCGACTCAGAGCGAGGAGTCGGCAATGGAGACCCTGAAGAAAATCTCGACCAGCAAAAGCATCAGAACCACCCTCGGCCGTTACATCAAGTGGATGATCGGGCAATGTAGCTCTGCTTCTCCGTCGCCATCCAAAACGGTGCGTTCGGCTCACGTTCAAAGCCCTGGTGCTACTTCACTCACTCCTCAGAGACTTCACTTCAGCCCTCCAGGTGAGGTAGTTAGTGACTGGTCACCTGTTGTTGGGCAATTACCTTATTGCTCTGCTTCTCCATCGAAAGCCGCGGTGACTTGTTCTTGTCAAAGATTGTCTGCTCATGCACAAGTTGATGAGAGGTCTCGAGGCGAGGCATTTAATCACCGGTCACCTGTTATTGAACAATCACCTTATTCCTCTGGTTCTCCATTGAAAGCTGCAGGCACTTCTTATCATAGACTGTTGTCTACTCCTGCACAAGCTGATGAGCAGTTTCATGGTGAGGCATTTAGTGCCCGGTCAGCTGTTGGACAATTACCTTGTTCCTCTGTTTCTCCATTAAAAGCCGCAGTCACTTCTTCTTGTCAAATATTGTCTACTCCTGTACAGCTTGATGAGAGGTTTCAAGTTGAGGCATTTAATGACCGGTCGCCTGTTATTGGACAATCACCTTATTCCTCTGGTTCTCCATTGAAAGCCACTAGCACTTCTTATCAAAGACTAATGTCTGCTGATGTTGTTCCTGTACAACTTCATGGAAGGTTTCAAGGTGAGGTTATCACTAGGTCCTCAGTTATAGACAAACCTGGAGATCATGGAGAAGCACACTTAGTAGCTCTGGGTGAACTAGAATTCCGTAAACAATTTCTGATTTTAAGTTATGCTGGAGGAACAAAGTTGAGAGATCTTATAGCGGCAGAGGACATTAGGCATTGGAAATATTTGCCAATGAATTTTGATTGGGATTCTGGACGCACACACGTCTATCATTGTTACGTCAACGTGGATGGGAGTTACAGATTTAAGAGAATCCTATCAGCATTGATTATCATCTTTCTGTCTTCATATGGTTATGAATCAGTGTTCAAAGATGGTGCCATAGACAAAGGTGTCAGTTATATATTATCTAACAGAACGATGTATGAAGCCAGATGTCTTTTCATGCATGCACATACTCTGGCTAGTATCGATAACTACATGGTTAGGTTCTCACTCATTTTGTCCAAGACAACGAGCTTAAAAGTAGATTGGTCAGTTGCCAAGGTTGAAATCATCGATGAAGAATACTGTCTCGATGAATCAGGTAATCGTATCCATAGAGATGAAAAACCACTTATACATACAGATGGAACTGGATTCATATCCGAGGATTTGGCTTTAATTTGTCCAAAGATAGAAGCCAAAGGACAATGTATAAGTGATGAACACATGAAGGGACTTCCTGATCCTGATGAACTTGAGGATAACGGTATGGGAAAGAAAAGGCCAGTTCTTAGAACTGAAGAACCGCCTTTGCTCACACAGTGCCGACTTTTTCACAATGGCAGTGCTTCTAAAGGAACGTTTCTTGTCAATAAAAAGATACTTAACCAAGCTTTGGAAGATGCTCATGGTGTTTTCTGCAAGACACGTGCAGCACTCAAAGGTGGTGATCTTATAGTACTTAGAATTGCTGAGAAGTATGGTGAGATCGATGACAATTTCTTGGGGAAATTAATGATCTCATTTGGAATACCTCTAGAAGAATCATACTTGCGGTATCGTCTCTCAGTCCTAATGAAAGAGGAAAACAACAGTCTTAGAAAAGGGAAACTTTATATTCCTGATTCCTATAATTTGATGGGGACAGCTGATCCAACAGGGACTTTAAAAAAGGATGAAGTCTGCGTTATCCTCTCTACATTAGCTCTGTGTTGTAGGAAGGATGGACAACTGTCGGGGAAGGTACTAGTGTACAGATATCCGGGTTTACACTTCGGGGATATTCATGTTCTAAAGGCAACCTATGTGAAGGAGTTAGAATCTTTTGTTGGAAATGCCAAATACGGTATATTCTTCTCTTGCAAAGGTCCACGTTCCATTGCTGATGAAATGGGCGGTGGAGATTTTGATGGTGATCTATACTGGGTCTCAAGAAACCCTCAGCTATTGGAGTGGTACGAACCAAGTGAACCTTGGATTGAAGCTTCAATATCAACCCAAAAAGTTCCCAGCAGGAGTCCATCTGACTATTCGCCTGAAGATCTAGAAGATCTTCTGTTTAGATCATTCTTGACAACTAGGTTTGAACCAAGTTTCGCTATGAGTGAGGCAGCTGATAACTGGCTAGCCTGGATGGATAGGTTCTTGACTCTAGGAGAGAGTGACATTGATGAAAAGAATGATATTAAGGAGAAGATACTGCAACTAATTAACTTATACTACACAGCTCTAGATGCACCAAAGAAAGGTGTAAAGGTTGTAGTCCCAAAAGAATTGAAGGTCCCATGTTTCCCCCATTACATGGGAAAGAACAATTCTTACCCTTCAACTTCCGTTTTGGGATTAATTTATGACAGGGTGGATGAGCATCAAACAGAAGACTGTTCTCTCAAAGAAGTCGAGAAACTTCCTGCTTTTGATATTAATGAAGTCCCTGATGCCTGCTTGGACAAATGGGAGGAACTACACCGACAGTACAGGAGTGAGATGGCCGCTGCTATGGACGATACTGATAAACAGAGCAGAAATCAGTCTGCTGATGTGGTCATTAGGAAATATAAAAAGATTCTGTACGACGCTAATGACTTTGAGGAGAGTAGAAGGCCGATCGATGAGATATATAACGAGGCACAAGCTATATATCATGTGTGTTATGACCATGCGAGGAGGGTAGGAAGTGTGAAGGCGTGTTCCTTTGCATGGAGAGTTGCAGGTAAAGCTCTGTGCCAGCTTCACGCCAAGAACACGAATGAAAGGTGTTGTACGATAGTATCCTCTGTTTTGAAGGAGATTTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RdRP

Protein Analysis

1034

Amino Acids

116.06

Weight (kDa)

6.08

Isoelectric Point (pI)

45.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RdRP PF05183 413 - 563 4.6e-07 RNA dependent RNA polymerase
RdRP PF05183 602 - 889 4.2e-55 RNA dependent RNA polymerase
RdRP_head PF26253 929 - 1018 4.7e-14 RdRP, head domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000505)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G19910 AT2G19910 AT2G19920 AT2G19920 AT2G19920 AT2G19920 AT2G19930 AT2G19930 AT2G19930
fragaria_vesca FvH4_1g05980 FvH4_1g05990 FvH4_1g05990 FvH4_1g09718
malus_domestica MD02G1064400.v1.1 MD15G1195300.v1.1 MD15G1195400.v1.1
prunus_persica Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1
pyrus_communis pycom02g05070 pycom15g17460
rosa_chinensis RchiOBHm_Chr2g0091671 RchiOBHm_Chr2g0091681 RchiOBHm_Chr2g0091691 RchiOBHm_Chr2g0091701 RchiOBHm_Chr2g0091711 RchiOBHm_Chr2g0091721 RchiOBHm_Chr2g0091731 RchiOBHm_Chr5g0014401
rosa_laevigata RLG00000013772 RLG00000016242 RLG00000016244 RLG00000016245 RLG00000016246 RLG00000032105
rosa_multiflora Rmu_co8374041.1_g000001 Rmu_co8478121.1_g000001 Rmu_sc0000897.1_g000005 Rmu_sc0000897.1_g000009
rosa_roxburghii Rroxscaffold_1G00062180 Rroxscaffold_1G00062190 Rroxscaffold_1G00068800 Rroxscaffold_2G00149790 Rroxscaffold_2G00149800 Rroxscaffold_2G00149810
rosa_rugosa Rorug02G0019600 Rorug02G0019700 Rorug02G0019800 Rorug02G0019900 Rorug02G0019900 Rorug02G0020000 Rorug02G0020000 Rorug02G0020100 Rorug02G0020100 Rorug02G0020200 Rorug05G0017800
rosa_samantha Rh2AG064700 Rh2AG064800 Rh2AG064900 Rh2BG063800 Rh2BG063900 Rh2BG064000 Rh2BG064100 Rh2CG065500 Rh2CG065600 Rh2CG065700 Rh2DG063900 Rh2DG064000 Rh2DG064100 Rh2DG064200 Rh2DG381700 Rh5AG056700 Rh5AG056800 Rh5AG056900 Rh5AG112600 Rh5BG109300 Rh5CG063800 Rh5CG121100 Rh5DG053100 Rh5DG108300 Rh6CG100100
rosa_wichuraiana Rw2G005560 Rw2G005580 Rw2G005590 Rw5G005370 Rw5G009770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1214
Acc36I ACCTGC 1 cut(s) 3008
AccB1I GGYRCC 2 cut(s) 34, 1199
AccB7I CCANNNNNTGG 2 cut(s) 1702, 2608
AccI GTMKAC 3 cut(s) 43, 572, 701
AccII CGCG 3 cut(s) 40, 131, 413
AciI CCGC 9 cut(s) 38, 411, 413, 672, 1019, 1613, 1861, 2202, 2799
AclI AACGTT 1 cut(s) 1667
AclWI GGATC 2 cut(s) 1544, 1952
AcoI YGGCCR 2 cut(s) 205, 2796
AcsI RAATTY 2 cut(s) 956, 1057
AcuI CTGAAG 4 cut(s) 185, 313, 1626, 2364
AcvI CACGTG 1 cut(s) 1736
AcyI GRCGYC 1 cut(s) 114
AdeI CACNNNGTG 1 cut(s) 1629
AfaI GTAC 9 cut(s) 711, 852, 1767, 2052, 2060, 2264, 2783, 2879, 3070
AfiI CCNNNNNNNGG 6 cut(s) 371, 764, 1702, 2552, 2608, 3006
AflIII ACRYGT 2 cut(s) 1084, 1733
AgeI ACCGGT 2 cut(s) 489, 750
AhlI ACTAGT 1 cut(s) 2053
AjiI CACGTC 1 cut(s) 1087
AjnI CCWGG 4 cut(s) 292, 337, 910, 2435
AjuI GAANNNNNNNTTGG 2 cut(s) 2118, 2150
AloI GAACNNNNNNTCC 2 cut(s) 942, 974
Alw26I GTCTC 6 cut(s) 152, 313, 471, 1400, 1874, 2239
AlwI GGATC 2 cut(s) 1544, 1952
Ama87I CYCGRG 1 cut(s) 468
AoxI GGCC 5 cut(s) 101, 205, 1589, 2796, 2909
ApeKI GCWGC 5 cut(s) 124, 542, 1739, 2417, 2801
ApoI RAATTY 2 cut(s) 956, 1057
AseI ATTAAT 3 cut(s) 1820, 2651, 2730
AsiGI ACCGGT 2 cut(s) 489, 750
Asp700I GAANNNNTTC 1 cut(s) 2358
AspS9I GGNCC 3 cut(s) 891, 2174, 2587
AsuC2I CCSGG 2 cut(s) 622, 2070
AsuNHI GCTAGC 1 cut(s) 2431
AvaI CYCGRG 1 cut(s) 468
AvaII GGWCC 3 cut(s) 891, 2174, 2587
BaeGI GKGCMC 1 cut(s) 622
BaeI ACNNNNGTAYC 4 cut(s) 2042, 2075, 3060, 3093
BanI GGYRCC 2 cut(s) 34, 1199
BarI GAAGNNNNNNTAC 2 cut(s) 229, 261
BbrPI CACGTG 1 cut(s) 1736
BbsI GAAGAC 2 cut(s) 1158, 2691
BbvCI CCTCAGC 1 cut(s) 2247
BbvI GCAGC 5 cut(s) 136, 529, 1751, 2429, 2788
BceAI ACGGC 1 cut(s) 192
BciT130I CCWGG 4 cut(s) 294, 339, 912, 2437
BciVI GTATCC 2 cut(s) 1427, 3088
BcnI CCSGG 2 cut(s) 622, 2070
BcoDI GTCTC 6 cut(s) 152, 313, 471, 1400, 1874, 2239
BcuI ACTAGT 1 cut(s) 2053
BfmI CTRYAG 1 cut(s) 543
BfuAI ACCTGC 1 cut(s) 3008
BfuI GTATCC 2 cut(s) 1427, 3088
BglII AGATCT 3 cut(s) 1009, 2347, 2356
BmcAI AGTACT 1 cut(s) 1767
Bme1390I CCNGG 6 cut(s) 294, 339, 622, 912, 2070, 2437
Bme18I GGWCC 3 cut(s) 891, 2174, 2587
BmeT110I CYCGRG 1 cut(s) 468
BmgBI CACGTC 1 cut(s) 1087
BmgT120I GGNCC 3 cut(s) 891, 2174, 2587
BmiI GGNNCC 3 cut(s) 36, 1201, 2589
BmrFI CCNGG 6 cut(s) 294, 339, 622, 912, 2070, 2437
BmrI ACTGGG 1 cut(s) 2239
BmsI GCATC 5 cut(s) 198, 1699, 2530, 2683, 2734
BmtI GCTAGC 1 cut(s) 2435
BmuI ACTGGG 1 cut(s) 2239
BoxI GACNNNNGTC 3 cut(s) 568, 829, 2038
BpiI GAAGAC 2 cut(s) 1158, 2691
BpmI CTGGAG 3 cut(s) 321, 933, 1011
Bpu10I CCTNAGC 1 cut(s) 2247
BpuEI CTTGAG 2 cut(s) 1583, 2221
BpuMI CCSGG 2 cut(s) 622, 2070
Bsa29I ATCGAT 4 cut(s) 1293, 1380, 1797, 2916
BsaAI YACGTR 1 cut(s) 1736
BsaBI GATNNNNATC 4 cut(s) 1154, 1465, 2220, 2673
BsaHI GRCGYC 1 cut(s) 114
BsaI GGTCTC 3 cut(s) 152, 471, 2239
BsaJI CCNNGG 7 cut(s) 98, 202, 292, 411, 1365, 1470, 2279
BsaWI WCCGGW 2 cut(s) 489, 750
BsaXI ACNNNNNCTCC 1 cut(s) 3086
Bsc4I CCNNNNNNNGG 6 cut(s) 371, 764, 1702, 2552, 2608, 3006
Bse118I RCCGGY 2 cut(s) 489, 750
Bse1I ACTGG 5 cut(s) 362, 1462, 1592, 2234, 2432
Bse3DI GCAATG 4 cut(s) 62, 159, 239, 2184
Bse8I GATNNNNATC 4 cut(s) 1154, 1465, 2220, 2673
BseBI CCWGG 4 cut(s) 294, 339, 912, 2437
BseCI ATCGAT 4 cut(s) 1293, 1380, 1797, 2916
BseDI CCNNGG 7 cut(s) 98, 202, 292, 411, 1365, 1470, 2279
BseGI GGATG 8 cut(s) 25, 228, 257, 1117, 1987, 2035, 2445, 2674
BseJI GATNNNNATC 4 cut(s) 1154, 1465, 2220, 2673
BseLI CCNNNNNNNGG 6 cut(s) 371, 764, 1702, 2552, 2608, 3006
BseMI GCAATG 4 cut(s) 62, 159, 239, 2184
BseMII CTCAG 7 cut(s) 62, 149, 329, 909, 1770, 1887, 2261
BseNI ACTGG 5 cut(s) 362, 1462, 1592, 2234, 2432
BseRI GAGGAG 4 cut(s) 158, 303, 2912, 2986
BseSI GKGCMC 1 cut(s) 622
BseX3I CGGCCG 1 cut(s) 205
BseXI GCAGC 5 cut(s) 136, 529, 1751, 2429, 2788
BseYI CCCAGC 1 cut(s) 2315
BsgI GTGCAG 2 cut(s) 564, 1758
Bsh1236I CGCG 3 cut(s) 40, 131, 413
Bsh1285I CGRYCG 3 cut(s) 208, 755, 2916
BshFI GGCC 5 cut(s) 103, 207, 1591, 2798, 2911
BshNI GGYRCC 2 cut(s) 34, 1199
BshTI ACCGGT 2 cut(s) 489, 750
BshVI ATCGAT 4 cut(s) 1293, 1380, 1797, 2916
BsiEI CGRYCG 3 cut(s) 208, 755, 2916
BsiHKCI CYCGRG 1 cut(s) 468
BsiSI CCGG 5 cut(s) 70, 490, 622, 751, 2069
BslFI GGGAC 6 cut(s) 1554, 1963, 1981, 2555, 2573, 2723
BslI CCNNNNNNNGG 6 cut(s) 371, 764, 1702, 2552, 2608, 3006
BsmAI GTCTC 6 cut(s) 152, 313, 471, 1400, 1874, 2239
BsmBI CGTCTC 1 cut(s) 1874
BsmFI GGGAC 6 cut(s) 1554, 1963, 1981, 2555, 2573, 2723
BsnI GGCC 5 cut(s) 103, 207, 1591, 2798, 2911
Bso31I GGTCTC 3 cut(s) 152, 471, 2239
BsoBI CYCGRG 1 cut(s) 468
Bsp1286I GDGCHC 1 cut(s) 622
Bsp1407I TGTACA 3 cut(s) 709, 850, 2058
Bsp68I TCGCGA 1 cut(s) 131
BspACI CCGC 9 cut(s) 38, 411, 413, 672, 1019, 1613, 1861, 2202, 2799
BspANI GGCC 5 cut(s) 103, 207, 1591, 2798, 2911
BspCNI CTCAG 7 cut(s) 61, 148, 328, 908, 1771, 1886, 2260
BspDI ATCGAT 4 cut(s) 1293, 1380, 1797, 2916
BspFNI CGCG 3 cut(s) 40, 131, 413
BspLI GGNNCC 3 cut(s) 36, 1201, 2589
BspMAI CTGCAG 1 cut(s) 547
BspMI ACCTGC 1 cut(s) 3008
BspOI GCTAGC 1 cut(s) 2435
BspPI GGATC 2 cut(s) 1544, 1952
BspT107I GGYRCC 2 cut(s) 34, 1199
BspTNI GGTCTC 3 cut(s) 152, 471, 2239
BsrDI GCAATG 4 cut(s) 62, 159, 239, 2184
BsrFI RCCGGY 2 cut(s) 489, 750
BsrGI TGTACA 3 cut(s) 709, 850, 2058
BsrI ACTGG 5 cut(s) 362, 1462, 1592, 2234, 2432
BssAI RCCGGY 2 cut(s) 489, 750
BssECI CCNNGG 7 cut(s) 98, 202, 292, 411, 1365, 1470, 2279
BssNI GRCGYC 1 cut(s) 114
BssT1I CCWWGG 3 cut(s) 98, 1365, 2279
Bst2UI CCWGG 4 cut(s) 294, 339, 912, 2437
Bst6I CTCTTC 1 cut(s) 24
BstACI GRCGYC 1 cut(s) 114
BstAPI GCANNNNNTGC 1 cut(s) 617
BstAUI TGTACA 3 cut(s) 709, 850, 2058
BstBAI YACGTR 1 cut(s) 1736
BstC8I GCNNGC 6 cut(s) 129, 547, 1272, 2433, 2749, 3035
BstDSI CCRYGG 1 cut(s) 411
BstEII GGTNACC 2 cut(s) 360, 492
BstF5I GGATG 8 cut(s) 25, 228, 257, 1117, 1987, 2035, 2445, 2674
BstFNI CGCG 3 cut(s) 40, 131, 413
BstMAI GTCTC 6 cut(s) 152, 313, 471, 1400, 1874, 2239
BstMCI CGRYCG 3 cut(s) 208, 755, 2916
BstMWI GCNNNNNNNGC 5 cut(s) 100, 617, 626, 2540, 3023
BstNI CCWGG 4 cut(s) 294, 339, 912, 2437
BstNSI RCATGY 1 cut(s) 1274
BstPAI GACNNNNGTC 3 cut(s) 568, 829, 2038
BstPI GGTNACC 2 cut(s) 360, 492
BstSCI CCNGG 6 cut(s) 292, 337, 620, 910, 2068, 2435
BstSFI CTRYAG 1 cut(s) 543
BstSLI GKGCMC 1 cut(s) 622
BstUI CGCG 3 cut(s) 40, 131, 413
BstV1I GCAGC 5 cut(s) 136, 529, 1751, 2429, 2788
BstV2I GAAGAC 2 cut(s) 1158, 2691
BstX2I RGATCY 3 cut(s) 1009, 2347, 2356
BstYI RGATCY 3 cut(s) 1009, 2347, 2356
BstZI CGGCCG 1 cut(s) 205
Bsu15I ATCGAT 4 cut(s) 1293, 1380, 1797, 2916
BsuI GTATCC 2 cut(s) 1427, 3088
BsuRI GGCC 5 cut(s) 103, 207, 1591, 2798, 2911
BsuTUI ATCGAT 4 cut(s) 1293, 1380, 1797, 2916
BtgI CCRYGG 1 cut(s) 411
BtrI CACGTC 1 cut(s) 1087
BtsCI GGATG 8 cut(s) 25, 228, 257, 1117, 1987, 2035, 2445, 2674
BtsI GCAGTG 1 cut(s) 1657
BtsIMutI CAGTG 3 cut(s) 1191, 1634, 1657
BtuMI TCGCGA 1 cut(s) 131
BveI ACCTGC 1 cut(s) 3008
Cac8I GCNNGC 6 cut(s) 129, 547, 1272, 2433, 2749, 3035
Cfr10I RCCGGY 2 cut(s) 489, 750
Cfr13I GGNCC 3 cut(s) 891, 2174, 2587
Cfr42I CCGCGG 1 cut(s) 414
ClaI ATCGAT 4 cut(s) 1293, 1380, 1797, 2916
CseI GACGC 5 cut(s) 29, 116, 122, 1086, 2891
Csp6I GTAC 9 cut(s) 710, 851, 1766, 2051, 2059, 2263, 2782, 2878, 3069
CspAI ACCGGT 2 cut(s) 489, 750
CviQI GTAC 9 cut(s) 710, 851, 1766, 2051, 2059, 2263, 2782, 2878, 3069
DraI TTTAAA 1 cut(s) 1974
DraIII CACNNNGTG 1 cut(s) 1629
DrdI GACNNNNNNGTC 1 cut(s) 1214
DseDI GACNNNNNNGTC 1 cut(s) 1214
EaeI YGGCCR 2 cut(s) 205, 2796
EagI CGGCCG 1 cut(s) 205
Eam1104I CTCTTC 1 cut(s) 24
EarI CTCTTC 1 cut(s) 24
EclXI CGGCCG 1 cut(s) 205
Eco130I CCWWGG 3 cut(s) 98, 1365, 2279
Eco31I GGTCTC 3 cut(s) 152, 471, 2239
Eco32I GATATC 1 cut(s) 2066
Eco47I GGWCC 3 cut(s) 891, 2174, 2587
Eco52I CGGCCG 1 cut(s) 205
Eco57I CTGAAG 4 cut(s) 185, 313, 1626, 2364
Eco72I CACGTG 1 cut(s) 1736
Eco88I CYCGRG 1 cut(s) 468
Eco91I GGTNACC 2 cut(s) 360, 492
EcoO109I RGGNCCY 2 cut(s) 891, 2587
EcoO65I GGTNACC 2 cut(s) 360, 492
EcoRI GAATTC 1 cut(s) 956
EcoRII CCWGG 4 cut(s) 292, 337, 910, 2435
EcoRV GATATC 1 cut(s) 2066
EcoT14I CCWWGG 3 cut(s) 98, 1365, 2279
EcoT22I ATGCAT 1 cut(s) 1272
ErhI CCWWGG 3 cut(s) 98, 1365, 2279
Esp3I CGTCTC 1 cut(s) 1874
FalI AAGNNNNNCTT 4 cut(s) 1902, 1934, 2263, 2295
FaqI GGGAC 6 cut(s) 1554, 1963, 1981, 2555, 2573, 2723
FauNDI CATATG 1 cut(s) 1171
FblI GTMKAC 3 cut(s) 43, 572, 701
FokI GGATG 8 cut(s) 32, 235, 244, 1124, 1994, 2042, 2452, 2681
GsaI CCCAGC 1 cut(s) 2319
GsuI CTGGAG 3 cut(s) 321, 933, 1011
HaeIII GGCC 5 cut(s) 103, 207, 1591, 2798, 2911
HapII CCGG 5 cut(s) 70, 490, 622, 751, 2069
HgaI GACGC 5 cut(s) 29, 116, 122, 1086, 2891
Hin1I GRCGYC 1 cut(s) 114
HincII GTYRAC 1 cut(s) 1105
HindII GTYRAC 1 cut(s) 1105
HindIII AAGCTT 2 cut(s) 1698, 2289
HpaII CCGG 5 cut(s) 70, 490, 622, 751, 2069
Hpy188I TCNGA 7 cut(s) 51, 138, 194, 318, 975, 1471, 2332
Hpy99I CGWCG 2 cut(s) 256, 2885
HpyCH4IV ACGT 7 cut(s) 282, 1086, 1101, 1107, 1667, 1735, 2179
HpyF10VI GCNNNNNNNGC 5 cut(s) 100, 617, 626, 2540, 3023
HpySE526I ACGT 7 cut(s) 282, 1086, 1101, 1107, 1667, 1735, 2179
Hsp92I GRCGYC 1 cut(s) 114
KspI CCGCGG 1 cut(s) 414
LmnI GCTCC 1 cut(s) 72
Lsp1109I GCAGC 5 cut(s) 136, 529, 1751, 2429, 2788
LweI GCATC 5 cut(s) 198, 1699, 2530, 2683, 2734
MaeII ACGT 7 cut(s) 282, 1086, 1101, 1107, 1667, 1735, 2179
MaeIII GTNAC 9 cut(s) 209, 353, 360, 415, 492, 676, 1097, 1118, 2468
MflI RGATCY 3 cut(s) 1009, 2347, 2356
MhlI GDGCHC 1 cut(s) 622
MlyI GAGTC 4 cut(s) 127, 155, 2332, 2449
MmeI TCCRAC 3 cut(s) 613, 1985, 2116
Mph1103I ATGCAT 1 cut(s) 1272
MroXI GAANNNNTTC 1 cut(s) 2358
MslI CAYNNNNRTG 2 cut(s) 1425, 1449
MspA1I CMGCKG 5 cut(s) 413, 629, 1955, 2420, 2801
MspI CCGG 5 cut(s) 70, 490, 622, 751, 2069
MspR9I CCNGG 6 cut(s) 294, 339, 622, 912, 2070, 2437
MvaI CCWGG 4 cut(s) 294, 339, 912, 2437
MvnI CGCG 3 cut(s) 40, 131, 413
MwoI GCNNNNNNNGC 5 cut(s) 100, 617, 626, 2540, 3023
NciI CCSGG 2 cut(s) 622, 2070
NdeI CATATG 1 cut(s) 1171
NheI GCTAGC 1 cut(s) 2431
NlaIV GGNNCC 3 cut(s) 36, 1201, 2589
NmeAIII GCCGAG 1 cut(s) 183
NmuCI GTSAC 6 cut(s) 353, 360, 415, 492, 676, 2468
NruI TCGCGA 1 cut(s) 131
NsiI ATGCAT 1 cut(s) 1272
NspI RCATGY 1 cut(s) 1274
PaeI GCATGC 1 cut(s) 1274
PaeR7I CTCGAG 1 cut(s) 468
PdmI GAANNNNTTC 1 cut(s) 2358
PfeI GAWTC 9 cut(s) 1070, 1134, 1181, 1403, 1461, 1850, 1931, 2126, 2872
PflMI CCANNNNNTGG 2 cut(s) 1702, 2608
PinAI ACCGGT 2 cut(s) 489, 750
Ple19I CGATCG 1 cut(s) 2916
PleI GAGTC 4 cut(s) 127, 154, 2331, 2449
PmaCI CACGTG 1 cut(s) 1736
PmlI CACGTG 1 cut(s) 1736
PpsI GAGTC 4 cut(s) 127, 154, 2331, 2449
Ppu21I YACGTR 1 cut(s) 1736
PpuMI RGGWCCY 2 cut(s) 891, 2587
PshAI GACNNNNGTC 3 cut(s) 568, 829, 2038
PshBI ATTAAT 3 cut(s) 1820, 2651, 2730
Psp1406I AACGTT 1 cut(s) 1667
Psp5II RGGWCCY 2 cut(s) 891, 2587
Psp6I CCWGG 4 cut(s) 292, 337, 910, 2435
PspCI CACGTG 1 cut(s) 1736
PspEI GGTNACC 2 cut(s) 360, 492
PspFI CCCAGC 1 cut(s) 2315
PspGI CCWGG 4 cut(s) 292, 337, 910, 2435
PspN4I GGNNCC 3 cut(s) 36, 1201, 2589
PspPI GGNCC 3 cut(s) 891, 2174, 2587
PspPPI RGGWCCY 2 cut(s) 891, 2587
PsrI GAACNNNNNNTAC 2 cut(s) 2609, 2641
PstI CTGCAG 1 cut(s) 547
PsuI RGATCY 3 cut(s) 1009, 2347, 2356
PvuI CGATCG 1 cut(s) 2916
PvuII CAGCTG 3 cut(s) 629, 1955, 2420
RruI TCGCGA 1 cut(s) 131
RsaI GTAC 9 cut(s) 711, 852, 1767, 2052, 2060, 2264, 2783, 2879, 3070
RsaNI GTAC 9 cut(s) 710, 851, 1766, 2051, 2059, 2263, 2782, 2878, 3069
RseI CAYNNNNRTG 2 cut(s) 1425, 1449
SacII CCGCGG 1 cut(s) 414
Sau96I GGNCC 3 cut(s) 891, 2174, 2587
ScaI AGTACT 1 cut(s) 1767
SchI GAGTC 4 cut(s) 127, 155, 2332, 2449
ScrFI CCNGG 6 cut(s) 294, 339, 622, 912, 2070, 2437
SduI GDGCHC 1 cut(s) 622
SfaNI GCATC 5 cut(s) 198, 1699, 2530, 2683, 2734
SfcI CTRYAG 1 cut(s) 543
Sfr274I CTCGAG 1 cut(s) 468
Sfr303I CCGCGG 1 cut(s) 414
SgrBI CCGCGG 1 cut(s) 414
SinI GGWCC 3 cut(s) 891, 2174, 2587
SlaI CTCGAG 1 cut(s) 468
SmiMI CAYNNNNRTG 2 cut(s) 1425, 1449
SmlI CTYRAG 3 cut(s) 468, 1562, 2236
SmoI CTYRAG 3 cut(s) 468, 1562, 2236
SpeI ACTAGT 1 cut(s) 2053
SphI GCATGC 1 cut(s) 1274
SsiI CCGC 9 cut(s) 38, 411, 413, 672, 1019, 1613, 1861, 2202, 2799
SspI AATATT 2 cut(s) 696, 1046
StyD4I CCNGG 6 cut(s) 292, 337, 620, 910, 2068, 2435
StyI CCWWGG 3 cut(s) 98, 1365, 2279
TaiI ACGT 7 cut(s) 285, 1089, 1104, 1110, 1670, 1738, 2182
TaqI TCGA 9 cut(s) 176, 404, 469, 1293, 1380, 1398, 1797, 2706, 2916
TatI WGTACW 5 cut(s) 709, 850, 1765, 2058, 2781
TauI GCSGC 5 cut(s) 40, 413, 674, 1022, 2801
TfiI GAWTC 9 cut(s) 1070, 1134, 1181, 1403, 1461, 1850, 1931, 2126, 2872
TscAI CASTG 3 cut(s) 1191, 1634, 1657
TseFI GTSAC 6 cut(s) 353, 360, 415, 492, 676, 2468
TseI GCWGC 5 cut(s) 124, 542, 1739, 2417, 2801
Tsp45I GTSAC 6 cut(s) 353, 360, 415, 492, 676, 2468
TspGWI ACGGA 3 cut(s) 240, 950, 2629
TspRI CASTG 3 cut(s) 1191, 1634, 1657
Van91I CCANNNNNTGG 2 cut(s) 1702, 2608
VpaK11BI GGWCC 3 cut(s) 891, 2174, 2587
VspI ATTAAT 3 cut(s) 1820, 2651, 2730
XapI RAATTY 2 cut(s) 956, 1057
XbaI TCTAGA 3 cut(s) 1843, 2350, 2536
XceI RCATGY 1 cut(s) 1274
XcmI CCANNNNNNNNNTGG 1 cut(s) 2277
XhoI CTCGAG 1 cut(s) 468
XmiI GTMKAC 3 cut(s) 43, 572, 701
XmnI GAANNNNTTC 1 cut(s) 2358
ZrmI AGTACT 1 cut(s) 1767
Zsp2I ATGCAT 1 cut(s) 1272
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.