pycom15g17460
ERF Family

Probably involved in the RNA silencing pathway and required for the generation of small interfering RNAs (siRNAs)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0000505
Physical Location & Seq
Forward (+)
12324470 .. 12330959
6490 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g17460.3

Sequence Viewer

Length: 3009 bp
ATGGCCATGGCGGATTCCACCGTCCCAATAAAAAACCCATCTTTCTCTCCTCCTCCGTCATATATGGCCGGCGGCGGCGGTCTAAATTTGCAACCGCAAACATATATTTTCCTGCCGCAGTCGGTGGAGCAGCTGCTGCGTCGAATTTGCGAAGAGAAAAAGCATCCCTGGCCAGAAGACGACGTTTTGCGGAGGCTGAGTTCGCTGACGGAGATGAAGGCACTCGAGCTCCTCCGTCAGATTGCCCGCGCCAAAGAAGTTAAGACTCTCAACGGTTACATCATCTGGCTCTGTCAGGCCCAGTATTCCTCCTCCTCTCCTTCGCAATCTTCTCTTGCTTCTCAATTCCAAGGTGTGACTCTCACTCATCGCTGCTCCTCAGCTGTTGAGCAACCTGTAGCTATAGGCAATGGAGAAGCAGAGCTGGAAGCTTTGGGAGAACTTGAATTCAGAAAACAGTTTCTGATTCTAAATTACTCTGGAGGAATGCAGTTGGGAAGTGTTATAGCGGCGGAGACTATTAGGAGTTGGAAAGATTTGCCAATGCATGTATTTGAGACCAGAGTTTGGGAAGCTTTGGGTCGCAATTATATCGGCAAGGAAGACCGGCGTTTGACTTTTGAATGGGATTCGGGAAAAACACACCTCTATCATTGTTATGTTTCTTTGGATGGGAGTTGCAAATTCAAGGGTCCGTTTCTAAACAAAGAAAGAACTCTCCTACAGAAAGTTTTAGGAGACGACAATGTTTTAATGGTGAAATTCGCTGATGAGGGGAATGGGACAGGTCCCTCAAATTCTTACAATAATAAGTATGCTACATATCGCAAGGTTGCAAGAGAAGGGATTCTTGTAGGGTTGCGACGATATCGTTTCTTTGTGTTCAAAGATGGTGGTAAAGAGGAAAAGATAAAAAACCCAACTTCATCACCCGTGAAGTGTTATTTTGTTCGAATGGACTCTGATGCTTCAATTGACAGGGATGTATGGCATATGTTAGCTAACAGAACAATTTATGAAGCAAGATCTCTTTTCATGCATGCCCATACTGTGTCTAGTGTGGCCACCTACATGGCTAGGTTTTCACTCATTCTGTCAAAGACAATCAGATTTCCAGTAGATCTGTCAGTTGTGAATGTTGAGTTTATTGATGAGGAATATTGTAGGGATGAATCTGGTAACCTTATTTACAGAGATGACAAACCGCTTATACATACAGATGGCACTGGATTTATATCTGAGGATTTGGCTTTACTTTGTCCGAATAACATATCCAAAGGAAAGCGTATCAGTAATAAAAAAATCAAGAGAATTCCGAATCATAATGAACTTGATGGCAAGGGTATGGAAAAGAAACAGCCTGGTTCTAGAATCGGGGAACCGCCTTTGCTCATACAGTTCCGACTGTTTTACAAAGGCTGTGCTGTTAAGGGAACGTTTCTTGTCAACAAAATGCTGCCACCGAAAACAGTCCAGATTCGACCTTCAATGGTTAAAGTTGAGATAGATCCTGAACTTTCAGATAATGAAACAGTGAATGCATTAGAGATAGTTGGAACAAGTATTCCACCCGGGAAGTCATATTTATCGAGGAATTTAATTGCGCTTCTATTCCATGGAGGGGTGCCAAAAGAATATTTCATGGAAATACTTGAGAGAGCTTTGGAAGATGCTCATAGTGTTTTCTGCAATGCACATACCGCACTCAAAGTTGCCGTTAACAATGGTGAGATTGATGACAATTCCAATACGGCAAGAATGATCCTATGCGGTATTCCCTTAGACGAATCATATTTGCAATATCGTCTATTGATATTAATAAAAGAGGAAATGAAAGGTCTTAGAAGAGGGAAACTGTATGTTCCTGATTGCTATTACTTGATGGGGACAGCTGATCCAACCGGGATTCTAGAGAAAGACGAAGTTTGCATTATCCTTGACGGCGGACAAGTTTCAGGCAAGGTATTAGTGTATCGGAATCCTGGTTTACACTTCGGGGATATACATGTTTTGAAGGCCACTTATGTGAAGGAGTTAGAATATGTTGTTGGAAATGCCAAGTATGCTATATTTTTCTCTCGCAAAGGCCCGCGTTCTGTAGCTGATGAAATAAGTGGTGGGGATTTTGATGGTGATCTCTACTGGGTTTCAAGAAACCCTCAGCTACTGGAGTATTTCAAACCCAGTGAACCTTGGGTTCCGGCTTCTTCAGCTCCTAAAGCTGCCAGTGTCAAACCAGCTAAGCTTTCGAAAGAAGACCTAGAAGAGCAGCTCATAAATCTGTTTTTGACAACTAGATTTAAACCAAGTTATGCAATGAGTGAGGCAGCTGATAGCTGGCTGGCTATGATGGATCGCTACTTGACCCGAGGAGATAGTAGCATTGATGAGAACTTAGTGAAGGCAAACATACTGCGTTTGGTTGATATATACTATGATGCGCTAGATGCGCCGAAGAAAGGTGGAAAGGTCGAAGTTCCCAAAGACTTGAAGGTGTCGAAGTTTCCTCATTACATGGAAAAGAAAAATTCATACCTTTCAACTTCAATTTTAGGATCGATTTATGACACGGTGAATGAGTACCAACCAGAGGACCTCTCAACCAGAGAAGTTAAGAAACTTCCCATTTTCGACGTTGAAGTCCCTGAGGAGTGCTTGATCAAATGGAGGGAACAATACAAACAGTACAGGTGTGACATGACCTCGGCATTGCGAGATGGTGATCGAGAGGGGAAGAATCAGGCTGCCGGGGAGCTCATAACAAAGTATAGAGAGATTCTGTACGGTGGTGATGATTTCGACAAGAGTACAAGGCCGATGGGTGAAATATTTGACGAGGCCATCGCGATATATAATATCTGTTACGACTATGCGAGGAGTGCAGGTGTAAGTAAATGCGGGTTTGCATGGAAAGTTGCAGGGTCAGCTCTCTTCAAACTTCACTGGATCAAACAAGGTGATGCATGCCCTTTTCTGGTGTCACCCTCTGTCTTGAAGGAAATTCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RdRP

Protein Analysis

1003

Amino Acids

113.08

Weight (kDa)

7.21

Isoelectric Point (pI)

44.13

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000505)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G19910 AT2G19910 AT2G19920 AT2G19920 AT2G19920 AT2G19920 AT2G19930 AT2G19930 AT2G19930
fragaria_vesca FvH4_1g05980 FvH4_1g05990 FvH4_1g05990 FvH4_1g09718
malus_domestica MD02G1064400.v1.1 MD15G1195300.v1.1 MD15G1195400.v1.1
prunus_persica Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1
pyrus_communis pycom02g05070 pycom15g17460
rosa_chinensis RchiOBHm_Chr2g0091671 RchiOBHm_Chr2g0091681 RchiOBHm_Chr2g0091691 RchiOBHm_Chr2g0091701 RchiOBHm_Chr2g0091711 RchiOBHm_Chr2g0091721 RchiOBHm_Chr2g0091731 RchiOBHm_Chr5g0014401
rosa_laevigata RLG00000013772 RLG00000016242 RLG00000016244 RLG00000016245 RLG00000016246 RLG00000032105
rosa_multiflora Rmu_co8374041.1_g000001 Rmu_co8478121.1_g000001 Rmu_sc0000897.1_g000005 Rmu_sc0000897.1_g000009
rosa_roxburghii Rroxscaffold_1G00062180 Rroxscaffold_1G00062190 Rroxscaffold_1G00068800 Rroxscaffold_2G00149790 Rroxscaffold_2G00149800 Rroxscaffold_2G00149810
rosa_rugosa Rorug02G0019600 Rorug02G0019700 Rorug02G0019800 Rorug02G0019900 Rorug02G0019900 Rorug02G0020000 Rorug02G0020000 Rorug02G0020100 Rorug02G0020100 Rorug02G0020200 Rorug05G0017800
rosa_samantha Rh2AG064700 Rh2AG064800 Rh2AG064900 Rh2BG063800 Rh2BG063900 Rh2BG064000 Rh2BG064100 Rh2CG065500 Rh2CG065600 Rh2CG065700 Rh2DG063900 Rh2DG064000 Rh2DG064100 Rh2DG064200 Rh2DG381700 Rh5AG056700 Rh5AG056800 Rh5AG056900 Rh5AG112600 Rh5BG109300 Rh5CG063800 Rh5CG121100 Rh5DG053100 Rh5DG108300 Rh6CG100100
rosa_wichuraiana Rw2G005560 Rw2G005580 Rw2G005590 Rw5G005370 Rw5G009770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2873
AasI GACNNNNNNGTC 1 cut(s) 2639
Acc36I ACCTGC 1 cut(s) 2873
AccB1I GGYRCC 1 cut(s) 1624
AccB7I CCANNNNNTGG 1 cut(s) 567
AccII CGCG 3 cut(s) 249, 2092, 2846
AclI AACGTT 1 cut(s) 1436
AclWI GGATC 6 cut(s) 1502, 1756, 1889, 2361, 2563, 2954
AcoI YGGCCR 4 cut(s) 3, 66, 170, 1062
AcuI CTGAAG 1 cut(s) 2193
AfaI GTAC 4 cut(s) 2582, 2687, 2783, 2809
AfiI CCNNNNNNNGG 5 cut(s) 567, 1621, 2459, 2590, 2974
AflIII ACRYGT 1 cut(s) 2005
AjnI CCWGG 3 cut(s) 167, 1360, 1981
AjuI GAANNNNNNNTTGG 2 cut(s) 2031, 2063
AleI CACNNNNGTG 1 cut(s) 2024
AloI GAACNNNNNNTCC 4 cut(s) 184, 216, 1549, 1581
Alw21I GWGCWC 2 cut(s) 231, 2757
Alw26I GTCTC 3 cut(s) 509, 551, 732
AlwI GGATC 6 cut(s) 1502, 1756, 1889, 2361, 2563, 2954
AlwNI CAGNNNCTG 3 cut(s) 136, 463, 2167
Ama87I CYCGRG 3 cut(s) 224, 1571, 2367
AoxI GGCC 9 cut(s) 3, 66, 170, 297, 1062, 2016, 2086, 2813, 2838
ApeKI GCWGC 9 cut(s) 130, 133, 136, 372, 1456, 2222, 2269, 2327, 2744
AseI ATTAAT 1 cut(s) 1817
Asp700I GAANNNNTTC 1 cut(s) 846
AspLEI GCGC 4 cut(s) 251, 1606, 2443, 2452
AspS9I GGNCC 5 cut(s) 298, 692, 788, 2087, 2593
AsuC2I CCSGG 4 cut(s) 1572, 1573, 1903, 2749
AsuII TTCGAA 2 cut(s) 952, 2249
AvaI CYCGRG 3 cut(s) 224, 1571, 2367
AvaII GGWCC 3 cut(s) 692, 788, 2593
AxyI CCTNAGG 1 cut(s) 2646
BalI TGGCCA 3 cut(s) 5, 172, 1064
BanI GGYRCC 1 cut(s) 1624
BanII GRGCYC 2 cut(s) 231, 2757
BbsI GAAGAC 3 cut(s) 183, 609, 2262
Bbv12I GWGCWC 2 cut(s) 231, 2757
BbvCI CCTCAGC 2 cut(s) 379, 2160
BbvI GCAGC 9 cut(s) 120, 123, 142, 359, 1443, 2209, 2281, 2339, 2731
BceAI ACGGC 3 cut(s) 1700, 1767, 1957
BcgI CGANNNNNNTGC 2 cut(s) 574, 608
BciT130I CCWGG 3 cut(s) 169, 1362, 1983
BclI TGATCA 1 cut(s) 2658
BcnI CCSGG 4 cut(s) 1572, 1573, 1903, 2749
BcoDI GTCTC 3 cut(s) 509, 551, 732
BfaI CTAG 7 cut(s) 1056, 1077, 1368, 1910, 2261, 2295, 2444
BfmI CTRYAG 4 cut(s) 396, 402, 722, 2097
BfuAI ACCTGC 1 cut(s) 2873
BglII AGATCT 2 cut(s) 1025, 1120
BlpI GCTNAGC 1 cut(s) 2241
Bme1390I CCNGG 7 cut(s) 169, 1362, 1572, 1573, 1903, 1983, 2749
Bme18I GGWCC 3 cut(s) 692, 788, 2593
BmeT110I CYCGRG 3 cut(s) 224, 1571, 2367
BmgT120I GGNCC 5 cut(s) 298, 692, 788, 2087, 2593
BmiI GGNNCC 5 cut(s) 693, 790, 1380, 1626, 2199
BmrFI CCNGG 7 cut(s) 169, 1362, 1572, 1573, 1903, 1983, 2749
BmrI ACTGGG 3 cut(s) 295, 2152, 2178
BmsI GCATC 6 cut(s) 172, 955, 1660, 2428, 2437, 2950
BmuI ACTGGG 3 cut(s) 295, 2152, 2178
BpiI GAAGAC 3 cut(s) 183, 609, 2262
BplI GAGNNNNNCTC 2 cut(s) 2582, 2614
BpmI CTGGAG 2 cut(s) 501, 2189
Bpu10I CCTNAGC 2 cut(s) 379, 2160
Bpu1102I GCTNAGC 1 cut(s) 2241
Bpu14I TTCGAA 2 cut(s) 952, 2249
BpuEI CTTGAG 1 cut(s) 1673
BpuMI CCSGG 4 cut(s) 1572, 1573, 1903, 2749
Bsa29I ATCGAT 1 cut(s) 2558
BsaBI GATNNNNATC 3 cut(s) 1234, 2133, 2721
BsaI GGTCTC 1 cut(s) 551
BsaJI CCNNGG 9 cut(s) 6, 167, 349, 1571, 1615, 2192, 2368, 2703, 2748
BsaXI ACNNNNNCTCC 6 cut(s) 184, 213, 214, 243, 2869, 2899
Bsc4I CCNNNNNNNGG 5 cut(s) 567, 1621, 2459, 2590, 2974
Bse118I RCCGGY 2 cut(s) 68, 606
Bse1I ACTGG 8 cut(s) 301, 1115, 1231, 2147, 2172, 2184, 2226, 2948
Bse21I CCTNAGG 1 cut(s) 2646
Bse3DI GCAATG 4 cut(s) 415, 1696, 2322, 2708
Bse8I GATNNNNATC 3 cut(s) 1234, 2133, 2721
BseBI CCWGG 3 cut(s) 169, 1362, 1983
BseCI ATCGAT 1 cut(s) 2558
BseDI CCNNGG 9 cut(s) 6, 167, 349, 1571, 1615, 2192, 2368, 2703, 2748
BseGI GGATG 4 cut(s) 163, 676, 988, 1174
BseJI GATNNNNATC 3 cut(s) 1234, 2133, 2721
BseLI CCNNNNNNNGG 5 cut(s) 567, 1621, 2459, 2590, 2974
BseMI GCAATG 4 cut(s) 415, 1696, 2322, 2708
BseMII CTCAG 5 cut(s) 188, 393, 1230, 2174, 2637
BseNI ACTGG 8 cut(s) 301, 1115, 1231, 2147, 2172, 2184, 2226, 2948
BseRI GAGGAG 9 cut(s) 39, 42, 221, 301, 304, 367, 2385, 2663, 2890
BseXI GCAGC 9 cut(s) 120, 123, 142, 359, 1443, 2209, 2281, 2339, 2731
BsgI GTGCAG 1 cut(s) 2901
Bsh1236I CGCG 3 cut(s) 249, 2092, 2846
BshFI GGCC 9 cut(s) 5, 68, 172, 299, 1064, 2018, 2088, 2815, 2840
BshNI GGYRCC 1 cut(s) 1624
BshVI ATCGAT 1 cut(s) 2558
BsiHKAI GWGCWC 2 cut(s) 231, 2757
BsiHKCI CYCGRG 3 cut(s) 224, 1571, 2367
BsiSI CCGG 6 cut(s) 69, 607, 1572, 1902, 2201, 2748
BslFI GGGAC 5 cut(s) 8, 774, 796, 1900, 2627
BslI CCNNNNNNNGG 5 cut(s) 567, 1621, 2459, 2590, 2974
BsmAI GTCTC 3 cut(s) 509, 551, 732
BsmBI CGTCTC 1 cut(s) 732
BsmFI GGGAC 5 cut(s) 8, 774, 796, 1900, 2627
BsmI GAATGC 2 cut(s) 492, 1543
BsnI GGCC 9 cut(s) 5, 68, 172, 299, 1064, 2018, 2088, 2815, 2840
Bso31I GGTCTC 1 cut(s) 551
BsoBI CYCGRG 3 cut(s) 224, 1571, 2367
Bsp119I TTCGAA 2 cut(s) 952, 2249
Bsp1286I GDGCHC 2 cut(s) 231, 2757
Bsp1720I GCTNAGC 1 cut(s) 2241
Bsp19I CCATGG 2 cut(s) 6, 1615
Bsp68I TCGCGA 1 cut(s) 2846
BspANI GGCC 9 cut(s) 5, 68, 172, 299, 1064, 2018, 2088, 2815, 2840
BspCNI CTCAG 5 cut(s) 189, 392, 1231, 2173, 2638
BspDI ATCGAT 1 cut(s) 2558
BspFNI CGCG 3 cut(s) 249, 2092, 2846
BspLI GGNNCC 5 cut(s) 693, 790, 1380, 1626, 2199
BspMI ACCTGC 1 cut(s) 2873
BspPI GGATC 6 cut(s) 1502, 1756, 1889, 2361, 2563, 2954
BspQI GCTCTTC 1 cut(s) 2259
BspT104I TTCGAA 2 cut(s) 952, 2249
BspT107I GGYRCC 1 cut(s) 1624
BspTNI GGTCTC 1 cut(s) 551
BsrDI GCAATG 4 cut(s) 415, 1696, 2322, 2708
BsrFI RCCGGY 2 cut(s) 68, 606
BsrI ACTGG 8 cut(s) 301, 1115, 1231, 2147, 2172, 2184, 2226, 2948
BssAI RCCGGY 2 cut(s) 68, 606
BssECI CCNNGG 9 cut(s) 6, 167, 349, 1571, 1615, 2192, 2368, 2703, 2748
BssT1I CCWWGG 4 cut(s) 6, 349, 1615, 2192
Bst2UI CCWGG 3 cut(s) 169, 1362, 1983
Bst6I CTCTTC 4 cut(s) 147, 1840, 2259, 2936
BstAPI GCANNNNNTGC 1 cut(s) 136
BstBI TTCGAA 2 cut(s) 952, 2249
BstC8I GCNNGC 7 cut(s) 70, 247, 1041, 2090, 2339, 2343, 2965
BstDEI CTNAG 9 cut(s) 197, 379, 1239, 1780, 1841, 2160, 2241, 2395, 2646
BstDSI CCRYGG 2 cut(s) 6, 1615
BstEII GGTNACC 1 cut(s) 1178
BstF5I GGATG 4 cut(s) 163, 676, 988, 1174
BstFNI CGCG 3 cut(s) 249, 2092, 2846
BstHHI GCGC 4 cut(s) 251, 1606, 2443, 2452
BstMAI GTCTC 3 cut(s) 509, 551, 732
BstNI CCWGG 3 cut(s) 169, 1362, 1983
BstNSI RCATGY 4 cut(s) 551, 1043, 2009, 2967
BstPI GGTNACC 1 cut(s) 1178
BstSCI CCNGG 7 cut(s) 167, 1360, 1570, 1571, 1901, 1981, 2747
BstSFI CTRYAG 4 cut(s) 396, 402, 722, 2097
BstUI CGCG 3 cut(s) 249, 2092, 2846
BstV1I GCAGC 9 cut(s) 120, 123, 142, 359, 1443, 2209, 2281, 2339, 2731
BstV2I GAAGAC 3 cut(s) 183, 609, 2262
BstX2I RGATCY 3 cut(s) 1025, 1120, 1507
BstXI CCANNNNNNTGG 1 cut(s) 1072
BstYI RGATCY 3 cut(s) 1025, 1120, 1507
Bsu15I ATCGAT 1 cut(s) 2558
Bsu36I CCTNAGG 1 cut(s) 2646
BsuRI GGCC 9 cut(s) 5, 68, 172, 299, 1064, 2018, 2088, 2815, 2840
BsuTUI ATCGAT 1 cut(s) 2558
BtgI CCRYGG 2 cut(s) 6, 1615
BtgZI GCGATG 2 cut(s) 353, 2827
BtsCI GGATG 4 cut(s) 163, 676, 988, 1174
BtsIMutI CAGTG 5 cut(s) 1224, 1539, 2191, 2233, 2941
BtuMI TCGCGA 1 cut(s) 2846
BveI ACCTGC 1 cut(s) 2873
Cac8I GCNNGC 7 cut(s) 70, 247, 1041, 2090, 2339, 2343, 2965
CaiI CAGNNNCTG 3 cut(s) 136, 463, 2167
CfoI GCGC 4 cut(s) 251, 1606, 2443, 2452
Cfr10I RCCGGY 2 cut(s) 68, 606
Cfr13I GGNCC 5 cut(s) 298, 692, 788, 2087, 2593
Cfr9I CCCGGG 1 cut(s) 1571
ClaI ATCGAT 1 cut(s) 2558
CseI GACGC 1 cut(s) 128
Csp6I GTAC 4 cut(s) 2581, 2686, 2782, 2808
CspCI CAANNNNNGTGG 2 cut(s) 874, 909
CviQI GTAC 4 cut(s) 2581, 2686, 2782, 2808
DdeI CTNAG 9 cut(s) 197, 379, 1239, 1780, 1841, 2160, 2241, 2395, 2646
DraI TTTAAA 1 cut(s) 2302
DrdI GACNNNNNNGTC 1 cut(s) 2639
DseDI GACNNNNNNGTC 1 cut(s) 2639
EaeI YGGCCR 4 cut(s) 3, 66, 170, 1062
Eam1104I CTCTTC 4 cut(s) 147, 1840, 2259, 2936
EarI CTCTTC 4 cut(s) 147, 1840, 2259, 2936
EciI GGCGGA 3 cut(s) 26, 527, 1959
Ecl136II GAGCTC 2 cut(s) 229, 2755
Eco130I CCWWGG 4 cut(s) 6, 349, 1615, 2192
Eco24I GRGCYC 2 cut(s) 231, 2757
Eco31I GGTCTC 1 cut(s) 551
Eco32I GATATC 1 cut(s) 869
Eco47I GGWCC 3 cut(s) 692, 788, 2593
Eco53kI GAGCTC 2 cut(s) 229, 2755
Eco57I CTGAAG 1 cut(s) 2193
Eco81I CCTNAGG 1 cut(s) 2646
Eco88I CYCGRG 3 cut(s) 224, 1571, 2367
Eco91I GGTNACC 1 cut(s) 1178
EcoICRI GAGCTC 2 cut(s) 229, 2755
EcoO109I RGGNCCY 2 cut(s) 788, 2593
EcoO65I GGTNACC 1 cut(s) 1178
EcoRI GAATTC 2 cut(s) 446, 1311
EcoRII CCWGG 3 cut(s) 167, 1360, 1981
EcoRV GATATC 1 cut(s) 869
EcoT14I CCWWGG 4 cut(s) 6, 349, 1615, 2192
EcoT22I ATGCAT 4 cut(s) 549, 1041, 1543, 2965
EcoT38I GRGCYC 2 cut(s) 231, 2757
ErhI CCWWGG 4 cut(s) 6, 349, 1615, 2192
Esp3I CGTCTC 1 cut(s) 732
FalI AAGNNNNNCTT 2 cut(s) 834, 866
FaqI GGGAC 5 cut(s) 8, 774, 796, 1900, 2627
FauI CCCGC 3 cut(s) 254, 2097, 2891
FauNDI CATATG 1 cut(s) 993
FbaI TGATCA 1 cut(s) 2658
FokI GGATG 4 cut(s) 150, 683, 995, 1181
FriOI GRGCYC 2 cut(s) 231, 2757
FspBI CTAG 7 cut(s) 1056, 1077, 1368, 1910, 2261, 2295, 2444
GlaI GCGC 4 cut(s) 250, 1605, 2442, 2451
GsuI CTGGAG 2 cut(s) 501, 2189
HaeIII GGCC 9 cut(s) 5, 68, 172, 299, 1064, 2018, 2088, 2815, 2840
HapII CCGG 6 cut(s) 69, 607, 1572, 1902, 2201, 2748
HgaI GACGC 1 cut(s) 128
HhaI GCGC 4 cut(s) 251, 1606, 2443, 2452
Hin6I GCGC 4 cut(s) 249, 1604, 2441, 2450
HinP1I GCGC 4 cut(s) 249, 1604, 2441, 2450
HincII GTYRAC 2 cut(s) 1447, 1720
HindII GTYRAC 2 cut(s) 1447, 1720
HindIII AAGCTT 3 cut(s) 429, 573, 2243
HpaI GTTAAC 1 cut(s) 1720
HpaII CCGG 6 cut(s) 69, 607, 1572, 1902, 2201, 2748
Hpy166II GTNNAC 4 cut(s) 1447, 1720, 1988, 2189
Hpy8I GTNNAC 4 cut(s) 1447, 1720, 1988, 2189
Hpy99I CGWCG 4 cut(s) 144, 185, 867, 2636
HpyAV CCTTC 9 cut(s) 211, 330, 836, 1494, 2008, 2023, 2395, 2485, 2989
HpyCH4IV ACGT 3 cut(s) 183, 1436, 2634
HpyF3I CTNAG 9 cut(s) 197, 379, 1239, 1780, 1841, 2160, 2241, 2395, 2646
HpySE526I ACGT 3 cut(s) 183, 1436, 2634
HspAI GCGC 4 cut(s) 249, 1604, 2441, 2450
KroI GCCGGC 1 cut(s) 68
KroNI GCCGGC 1 cut(s) 70
Ksp22I TGATCA 1 cut(s) 2658
KspAI GTTAAC 1 cut(s) 1720
LguI GCTCTTC 1 cut(s) 2259
LmnI GCTCC 5 cut(s) 127, 234, 380, 2218, 2752
Lsp1109I GCAGC 9 cut(s) 120, 123, 142, 359, 1443, 2209, 2281, 2339, 2731
LweI GCATC 6 cut(s) 172, 955, 1660, 2428, 2437, 2950
MaeI CTAG 7 cut(s) 1056, 1077, 1368, 1910, 2261, 2295, 2444
MaeII ACGT 3 cut(s) 183, 1436, 2634
MaeIII GTNAC 6 cut(s) 275, 355, 1178, 2693, 2861, 2979
MfeI CAATTG 1 cut(s) 972
MflI RGATCY 3 cut(s) 1025, 1120, 1507
MhlI GDGCHC 2 cut(s) 231, 2757
MlsI TGGCCA 3 cut(s) 5, 172, 1064
MluNI TGGCCA 3 cut(s) 5, 172, 1064
MlyI GAGTC 3 cut(s) 259, 352, 953
MmeI TCCRAC 5 cut(s) 509, 1426, 1534, 1922, 2029
Mox20I TGGCCA 3 cut(s) 5, 172, 1064
Mph1103I ATGCAT 4 cut(s) 549, 1041, 1543, 2965
MroNI GCCGGC 1 cut(s) 68
MroXI GAANNNNTTC 1 cut(s) 846
MscI TGGCCA 3 cut(s) 5, 172, 1064
MseI TTAA 9 cut(s) 261, 752, 1428, 1494, 1598, 1719, 1817, 2301, 2613
MslI CAYNNNNRTG 4 cut(s) 657, 1070, 1218, 2024
Msp20I TGGCCA 3 cut(s) 5, 172, 1064
MspA1I CMGCKG 4 cut(s) 133, 383, 1892, 2330
MspI CCGG 6 cut(s) 69, 607, 1572, 1902, 2201, 2748
MspR9I CCNGG 7 cut(s) 169, 1362, 1572, 1573, 1903, 1983, 2749
MunI CAATTG 1 cut(s) 972
Mva1269I GAATGC 2 cut(s) 492, 1543
MvaI CCWGG 3 cut(s) 169, 1362, 1983
MvnI CGCG 3 cut(s) 249, 2092, 2846
NaeI GCCGGC 1 cut(s) 70
NciI CCSGG 4 cut(s) 1572, 1573, 1903, 2749
NcoI CCATGG 2 cut(s) 6, 1615
NdeI CATATG 1 cut(s) 993
NgoMIV GCCGGC 1 cut(s) 68
NlaIV GGNNCC 5 cut(s) 693, 790, 1380, 1626, 2199
NmeAIII GCCGAG 1 cut(s) 2684
NmuCI GTSAC 3 cut(s) 355, 2693, 2979
NruI TCGCGA 1 cut(s) 2846
NsiI ATGCAT 4 cut(s) 549, 1041, 1543, 2965
NspI RCATGY 4 cut(s) 551, 1043, 2009, 2967
NspV TTCGAA 2 cut(s) 952, 2249
OliI CACNNNNGTG 1 cut(s) 2024
PaeI GCATGC 2 cut(s) 1043, 2967
PaeR7I CTCGAG 1 cut(s) 224
PaqCI CACCTGC 1 cut(s) 2873
PciI ACATGT 1 cut(s) 2005
PciSI GCTCTTC 1 cut(s) 2259
PctI GAATGC 2 cut(s) 492, 1543
PdiI GCCGGC 1 cut(s) 70
PdmI GAANNNNTTC 1 cut(s) 846
PflMI CCANNNNNTGG 1 cut(s) 567
PleI GAGTC 3 cut(s) 259, 352, 953
PpsI GAGTC 3 cut(s) 259, 352, 953
PpuMI RGGWCCY 2 cut(s) 788, 2593
PscI ACATGT 1 cut(s) 2005
PshBI ATTAAT 1 cut(s) 1817
Psp124BI GAGCTC 2 cut(s) 231, 2757
Psp1406I AACGTT 1 cut(s) 1436
Psp5II RGGWCCY 2 cut(s) 788, 2593
Psp6I CCWGG 3 cut(s) 167, 1360, 1981
PspEI GGTNACC 1 cut(s) 1178
PspGI CCWGG 3 cut(s) 167, 1360, 1981
PspN4I GGNNCC 5 cut(s) 693, 790, 1380, 1626, 2199
PspPI GGNCC 5 cut(s) 298, 692, 788, 2087, 2593
PspPPI RGGWCCY 2 cut(s) 788, 2593
PspXI VCTCGAGB 1 cut(s) 224
PstNI CAGNNNCTG 3 cut(s) 136, 463, 2167
PsuI RGATCY 3 cut(s) 1025, 1120, 1507
PvuII CAGCTG 4 cut(s) 133, 383, 1892, 2330
RruI TCGCGA 1 cut(s) 2846
RsaI GTAC 4 cut(s) 2582, 2687, 2783, 2809
RsaNI GTAC 4 cut(s) 2581, 2686, 2782, 2808
RseI CAYNNNNRTG 4 cut(s) 657, 1070, 1218, 2024
SacI GAGCTC 2 cut(s) 231, 2757
SapI GCTCTTC 1 cut(s) 2259
SaqAI TTAA 9 cut(s) 261, 752, 1428, 1494, 1598, 1719, 1817, 2301, 2613
Sau96I GGNCC 5 cut(s) 298, 692, 788, 2087, 2593
SchI GAGTC 3 cut(s) 259, 352, 953
ScrFI CCNGG 7 cut(s) 169, 1362, 1572, 1573, 1903, 1983, 2749
SduI GDGCHC 2 cut(s) 231, 2757
SfaNI GCATC 6 cut(s) 172, 955, 1660, 2428, 2437, 2950
SfcI CTRYAG 4 cut(s) 396, 402, 722, 2097
Sfr274I CTCGAG 1 cut(s) 224
SfuI TTCGAA 2 cut(s) 952, 2249
SinI GGWCC 3 cut(s) 692, 788, 2593
SlaI CTCGAG 1 cut(s) 224
SmaI CCCGGG 1 cut(s) 1573
SmiMI CAYNNNNRTG 4 cut(s) 657, 1070, 1218, 2024
SmlI CTYRAG 2 cut(s) 224, 1652
SmoI CTYRAG 2 cut(s) 224, 1652
SphI GCATGC 2 cut(s) 1043, 2967
SspI AATATT 3 cut(s) 1160, 1637, 2829
SspMI CTAG 7 cut(s) 1056, 1077, 1368, 1910, 2261, 2295, 2444
SstI GAGCTC 2 cut(s) 231, 2757
StyD4I CCNGG 7 cut(s) 167, 1360, 1570, 1571, 1901, 1981, 2747
StyI CCWWGG 4 cut(s) 6, 349, 1615, 2192
TaiI ACGT 3 cut(s) 186, 1439, 2637
TatI WGTACW 2 cut(s) 2685, 2807
TauI GCSGC 4 cut(s) 75, 78, 118, 512
Tru1I TTAA 9 cut(s) 261, 752, 1428, 1494, 1598, 1719, 1817, 2301, 2613
Tru9I TTAA 9 cut(s) 261, 752, 1428, 1494, 1598, 1719, 1817, 2301, 2613
TscAI CASTG 5 cut(s) 1231, 1539, 2191, 2233, 2948
TseFI GTSAC 3 cut(s) 355, 2693, 2979
TseI GCWGC 9 cut(s) 130, 133, 136, 372, 1456, 2222, 2269, 2327, 2744
Tsp45I GTSAC 3 cut(s) 355, 2693, 2979
TspGWI ACGGA 4 cut(s) 45, 224, 224, 684
TspMI CCCGGG 1 cut(s) 1571
TspRI CASTG 5 cut(s) 1231, 1539, 2191, 2233, 2948
Van91I CCANNNNNTGG 1 cut(s) 567
VpaK11BI GGWCC 3 cut(s) 692, 788, 2593
VspI ATTAAT 1 cut(s) 1817
XbaI TCTAGA 2 cut(s) 1367, 1909
XceI RCATGY 4 cut(s) 551, 1043, 2009, 2967
XhoI CTCGAG 1 cut(s) 224
XmaI CCCGGG 1 cut(s) 1571
XmnI GAANNNNTTC 1 cut(s) 846
XspI CTAG 7 cut(s) 1056, 1077, 1368, 1910, 2261, 2295, 2444
Zsp2I ATGCAT 4 cut(s) 549, 1041, 1543, 2965
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.