Rh2DG064100
ERF Family

Probably involved in the RNA silencing pathway and required for the generation of small interfering RNAs (siRNAs)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0000505
Physical Location & Seq
Forward (+)
4808421 .. 4819155
10735 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG064100.1

Sequence Viewer

Length: 2994 bp
ATGATGAAGCAGAGTTGGGATGAGCAAGAAGAGGTGCCGCGTCTACCTCAGAAAGTGGAGGTATTGCTCCGGCAAATCTGTGTGGACAAAAAGCAACCTTGGCCGGACGCTGACGCCATAAGGCAGCTCGCGACTCAGAGCGAGGAGTCGGCAATGGAGACCCTGGAGAAAATCTCCACCAGCAAAAGCATCAGAACCCTCGGCGGTTATATAAAGTGGATGATCCGGGAATGTAGCTCTGCTTCTCCGTCGCCGTCCAAAACGGTGCGTTCCGCTCACGTTCAAAGCCCTGGTGCTACTTCACTCACTCCTCAGAGACTTCACTTCAGCCCTCCAGGTGAGGGTATCACTAGGTCCTCAGCTATAGACAAACCTGGAGATCATGGAGAAGCACACTTAGTAGCTCTGGGTGAACTAGAATTCCGTAAACAATTTCTGATTTTAAGTTATGCTGGAGGAACGAAGTTGAGAGATCTTATAGCGGCAGAGGACATTAGGGATTGGAAATATTTGCCAATGGTAATGTTTGAGAGCAAAATTTGGGAGTATCTAGGTCGAAAATGCATCAGCCTCGAAGACCGTCGATTGAATTTTGATTGGGATTCTGGACGCACACACGTCTATCATTGTTACGTCAACGTGGATGGGAGTTACATATTTAAGGGCCCATATTTATATAAAATAAGAAGAACGCTCCTACAGAAAAAATTAGGAGATGACAATGTTTTAATGGTGAAGTTTGCCAAAGAAGAGGTGCAGAGAGGTCAAAGTATTGCCTGCCATCATAATTATTCTATGTATATGAAGGTTGCAAGAGAAGGGATTCTTGTTGGTTTGCGGCGATATCGTTTCTTTGTGTTCAAAGATGGTGGTAATGAGGAGAAGAAAAAAAACCCAACTTCATCACCTGTGAAGTGTTACTTTGTTCGAGTGACCTCTGATGCAGCCATAGACAAAGGTGTCAGTTATATATTATCTAACAGAACGATGTATGAAGCCAGATGTCTTTTCATGCATGCACATACTCTGGCTAGGATCGATAACTACATGGTTAGGTTCTCACTCATTTTGTCCAAGACAACAAGCTTAAAAGTAGATTGGTCAGTTGTCAAGGTTGAAATCATCGGTGAAGAATACTGTCTCGATGAATCTGGTAATCGTATCCATAGAGATGAAAAACCACTTATACATACAGATGGAACTGGATTCATATCCGAGGATTTGGCTTTAATTTGTCCAAAGATAGAAGCCAAAGGACAATGTATAAGTGATGAACACATGAAGGGACTTCCTGATCCTGATGAACTTGAGGATGATGATATGGGAAAGAAAAGGCCAGTTCTTAGAACAGACGAACCGCCTTTGCTCACACAGTGCCGACTTTTTCACAATGGCAGTGCTTCTAAAGGAACGTTTCTTGTCAATAAAAAGCTCCAGCCGAACACAATCCAGATTCGACCATCCATGCTCAAAGTTAAGACAGATCCAACGATTTCCAATAATCAAACAATAAACTCATTGGAAATAGTTGGCACAAGCACTCCTCCTAGGAATACGCACTTATCGAGGATTTTAATTGCACTTCTAATGCATGGAGGGGTACCGGAAGAATTTTTCATGCAGATACTTAACCAAGCTTTGGAAGATGCTCATGGTGTTTTCTGCAAGACACGTGCAGCACTCAAAGTTGCTGAGAAGTATGGTGAGATTGATGACAATTTCTTGGGGAAATTAATGATCTCATTTGGAATACCTCTAGAAGAATCATACTTGCGGTATCGTCTCTCAGTCCTAATGAAAGAGGAAAACAACAGTCTTAGAAAAGGGAAACTTTATATTCCTGATTCCTATAATTTGATGGGGACAGCTGATCCAACAGGGACTTTAAAAAAAGATGAAGTCTGTGTTATCCTGAAGGATGGACAACTGTCGGGGAAGGTACTAGTCTACAGATATCCGGGTTTACACTTTGGGGATATTCATGTTCTAAAGGCGACCTATGTGAAGGAGTTAGAATCTTTTGTTGGAAATGCCAAATACGGTATATTCTTCTCTTGCAAAGGTCCACGTTCCATTGCTGATGAAATGGGTGGTGGAGATTTTGATGGTGATCTATACTGGGTCTCAAGAAACCCTCAGCTATTGGAGTGGTACAAACCAAGTGAACCTTGGATTGAAGCTTCAATACCAACCCAAAAAGTTCCCAGTAGGAGTCCATCTGACTATTCGCCTGAAGATCTAGAAGATCTTCTGTTTAGATCATTCTTGACAACGAGGTTTGAACCAAGTTTCGCTATGAGTGAGGCAGCTGATAATTGGCTAGCCTGGATGGATCGTTTCTTGACATTGGGAGAGAGTGACATTGACGAAAAGAATAATATGAAGAAAAAGATACTGCAACTAATTAACTTATACTACACAGCTCTAGATGCACCGAAGAAAGGTGTAAAGGTTGTAGTCCCAAAAGAATTGAAGGCCCCATGTTTCCCCCATTACATGGGAAAGAACAATTCTTACCCTTCAACTTCCGTTTTGGGATTAATTTATAACAGGGTGGATGAGCATCAAACAGAAGACTGTTCTCTCAAAGAAGTCGAGAAACTTCCTGCTTTTGATGTTAATGAAGTCCCTGATGCCTGCTTGGACAAATGGGAGGAACTACACCGACAGTACAGGAGTGAGATGGCCTCTGCTATGGACGATGCTGATAAACAGAGCAAAAATCAGTCTGCCGATGTGGTCATAAGGAAATATAAAAAGATTCTTTACGAGGCTAATGACTTTGAGGAGAGTAGAAGGCCAATCGATGAGATATATAACGAGGCACAAGCTATATATCATGTGTGTTATGACCATGCGAGGAGGATAGGAAGTGTGAAGGCGTGTTCCTTTGCATGGAGAGTTGCAGGTAAAGCTCTGTGCCAGCTTCACGCCAAGAACACGAATGAAAGGTGTTGTACGATAGTATCCTCTGTTTTGAAGGAGATTTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RdRP

Protein Analysis

997

Amino Acids

113.59

Weight (kDa)

7.1

Isoelectric Point (pI)

43.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
4HB_RdRP3_N PF26249 15 - 83 1.1e-06 RdRP3/4/5, N-terminal four helical bundle domain
RdRP_helical PF26252 132 - 196 2.8e-07 RNA dependent RNA polymerase, helical domain
RdRP PF05183 218 - 852 9.1e-100 RNA dependent RNA polymerase
RdRP_head PF26253 871 - 981 3e-14 RdRP, head domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000505)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G19910 AT2G19910 AT2G19920 AT2G19920 AT2G19920 AT2G19920 AT2G19930 AT2G19930 AT2G19930
fragaria_vesca FvH4_1g05980 FvH4_1g05990 FvH4_1g05990 FvH4_1g09718
malus_domestica MD02G1064400.v1.1 MD15G1195300.v1.1 MD15G1195400.v1.1
prunus_persica Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1
pyrus_communis pycom02g05070 pycom15g17460
rosa_chinensis RchiOBHm_Chr2g0091671 RchiOBHm_Chr2g0091681 RchiOBHm_Chr2g0091691 RchiOBHm_Chr2g0091701 RchiOBHm_Chr2g0091711 RchiOBHm_Chr2g0091721 RchiOBHm_Chr2g0091731 RchiOBHm_Chr5g0014401
rosa_laevigata RLG00000013772 RLG00000016242 RLG00000016244 RLG00000016245 RLG00000016246 RLG00000032105
rosa_multiflora Rmu_co8374041.1_g000001 Rmu_co8478121.1_g000001 Rmu_sc0000897.1_g000005 Rmu_sc0000897.1_g000009
rosa_roxburghii Rroxscaffold_1G00062180 Rroxscaffold_1G00062190 Rroxscaffold_1G00068800 Rroxscaffold_2G00149790 Rroxscaffold_2G00149800 Rroxscaffold_2G00149810
rosa_rugosa Rorug02G0019600 Rorug02G0019700 Rorug02G0019800 Rorug02G0019900 Rorug02G0019900 Rorug02G0020000 Rorug02G0020000 Rorug02G0020100 Rorug02G0020100 Rorug02G0020200 Rorug05G0017800
rosa_samantha Rh2AG064700 Rh2AG064800 Rh2AG064900 Rh2BG063800 Rh2BG063900 Rh2BG064000 Rh2BG064100 Rh2CG065500 Rh2CG065600 Rh2CG065700 Rh2DG063900 Rh2DG064000 Rh2DG064100 Rh2DG064200 Rh2DG381700 Rh5AG056700 Rh5AG056800 Rh5AG056900 Rh5AG112600 Rh5BG109300 Rh5CG063800 Rh5CG121100 Rh5DG053100 Rh5DG108300 Rh6CG100100
rosa_wichuraiana Rw2G005560 Rw2G005580 Rw2G005590 Rw5G005370 Rw5G009770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2547
AasI GACNNNNNNGTC 1 cut(s) 959
Acc36I ACCTGC 1 cut(s) 2897
Acc65I GGTACC 1 cut(s) 1600
AccB1I GGYRCC 2 cut(s) 34, 1600
AccB7I CCANNNNNTGG 2 cut(s) 1639, 2497
AccBSI CCGCTC 1 cut(s) 275
AccI GTMKAC 2 cut(s) 43, 1947
AccII CGCG 2 cut(s) 40, 131
AciI CCGC 7 cut(s) 38, 204, 273, 482, 838, 1358, 1774
AclI AACGTT 1 cut(s) 1412
AclWI GGATC 6 cut(s) 217, 1043, 1289, 1478, 1865, 2340
AcoI YGGCCR 1 cut(s) 101
AcsI RAATTY 4 cut(s) 419, 537, 589, 1610
AcuI CTGAAG 3 cut(s) 310, 1934, 2253
AcvI CACGTG 1 cut(s) 1673
AcyI GRCGYC 1 cut(s) 114
AdeI CACNNNGTG 1 cut(s) 1374
AfaI GTAC 5 cut(s) 1602, 1941, 2153, 2672, 2959
AfiI CCNNNNNNNGG 5 cut(s) 341, 1639, 2441, 2497, 2895
AflIII ACRYGT 2 cut(s) 616, 1670
AhlI ACTAGT 1 cut(s) 1942
AjiI CACGTC 1 cut(s) 619
AjnI CCWGG 5 cut(s) 162, 289, 334, 373, 2324
AjuI GAANNNNNNNTTGG 2 cut(s) 2007, 2039
AloI GAACNNNNNNTCC 2 cut(s) 405, 437
Alw26I GTCTC 5 cut(s) 152, 310, 1145, 1787, 2128
AlwI GGATC 6 cut(s) 217, 1043, 1289, 1478, 1865, 2340
AoxI GGCC 6 cut(s) 101, 664, 1334, 2475, 2685, 2798
ApaI GGGCCC 1 cut(s) 668
ApeKI GCWGC 4 cut(s) 124, 944, 1676, 2306
ApoI RAATTY 4 cut(s) 419, 537, 589, 1610
AseI ATTAAT 2 cut(s) 1733, 2540
Asp700I GAANNNNTTC 2 cut(s) 822, 2247
Asp718I GGTACC 1 cut(s) 1600
AspA2I CCTAGG 1 cut(s) 1547
AspS9I GGNCC 5 cut(s) 354, 664, 665, 2063, 2476
AsuC2I CCSGG 2 cut(s) 227, 1959
AsuHPI GGTGA 7 cut(s) 350, 422, 745, 897, 1139, 1715, 2120
AsuNHI GCTAGC 1 cut(s) 2320
AvaII GGWCC 2 cut(s) 354, 2063
AvrII CCTAGG 1 cut(s) 1547
BaeGI GKGCMC 1 cut(s) 668
BaeI ACNNNNGTAYC 2 cut(s) 2949, 2982
BanI GGYRCC 2 cut(s) 34, 1600
BanII GRGCYC 1 cut(s) 668
BarI GAAGNNNNNNTAC 4 cut(s) 226, 258, 2169, 2201
BbrPI CACGTG 1 cut(s) 1673
BbsI GAAGAC 2 cut(s) 582, 2580
BbvCI CCTCAGC 2 cut(s) 358, 2136
BbvI GCAGC 4 cut(s) 136, 956, 1688, 2318
BceAI ACGGC 1 cut(s) 238
BcgI CGANNNNNNTGC 2 cut(s) 553, 587
BciT130I CCWGG 5 cut(s) 164, 291, 336, 375, 2326
BciVI GTATCC 2 cut(s) 1172, 2977
BcnI CCSGG 2 cut(s) 227, 1959
BcoDI GTCTC 5 cut(s) 152, 310, 1145, 1787, 2128
BcuI ACTAGT 1 cut(s) 1942
BfmI CTRYAG 3 cut(s) 363, 698, 1948
BfuAI ACCTGC 1 cut(s) 2897
BfuI GTATCC 2 cut(s) 1172, 2977
BglII AGATCT 3 cut(s) 472, 2236, 2245
BisI GCNGC 7 cut(s) 38, 125, 483, 839, 945, 1677, 2307
BlnI CCTAGG 1 cut(s) 1547
BlsI GCNGC 7 cut(s) 39, 126, 484, 840, 946, 1678, 2308
Bme1390I CCNGG 7 cut(s) 164, 227, 291, 336, 375, 1959, 2326
Bme18I GGWCC 2 cut(s) 354, 2063
BmgBI CACGTC 1 cut(s) 619
BmgT120I GGNCC 5 cut(s) 354, 664, 665, 2063, 2476
BmiI GGNNCC 4 cut(s) 36, 666, 1602, 2478
BmrFI CCNGG 7 cut(s) 164, 227, 291, 336, 375, 1959, 2326
BmrI ACTGGG 2 cut(s) 2128, 2199
BmsI GCATC 8 cut(s) 198, 573, 931, 1636, 2419, 2572, 2623, 2692
BmtI GCTAGC 1 cut(s) 2324
BmuI ACTGGG 2 cut(s) 2128, 2199
BoxI GACNNNNGTC 1 cut(s) 1927
BpiI GAAGAC 2 cut(s) 582, 2580
BplI GAGNNNNNCTC 4 cut(s) 158, 190, 2672, 2704
BpmI CTGGAG 5 cut(s) 185, 318, 396, 474, 1418
Bpu10I CCTNAGC 2 cut(s) 358, 2136
BpuEI CTTGAG 2 cut(s) 1328, 2110
BpuMI CCSGG 2 cut(s) 227, 1959
Bsa29I ATCGAT 2 cut(s) 1038, 2805
BsaAI YACGTR 1 cut(s) 1673
BsaBI GATNNNNATC 3 cut(s) 1210, 2109, 2562
BsaHI GRCGYC 1 cut(s) 114
BsaI GGTCTC 2 cut(s) 152, 2128
BsaJI CCNNGG 7 cut(s) 98, 162, 199, 289, 1215, 1547, 2168
BsaWI WCCGGW 1 cut(s) 1603
BsaXI ACNNNNNCTCC 3 cut(s) 1525, 1555, 2975
Bsc4I CCNNNNNNNGG 5 cut(s) 341, 1639, 2441, 2497, 2895
Bse1I ACTGG 4 cut(s) 1207, 1337, 2123, 2205
Bse3DI GCAATG 2 cut(s) 159, 2073
Bse8I GATNNNNATC 3 cut(s) 1210, 2109, 2562
BseBI CCWGG 5 cut(s) 164, 291, 336, 375, 2326
BseCI ATCGAT 2 cut(s) 1038, 2805
BseDI CCNNGG 7 cut(s) 98, 162, 199, 289, 1215, 1547, 2168
BseGI GGATG 8 cut(s) 25, 225, 649, 1318, 1460, 1924, 2334, 2563
BseJI GATNNNNATC 3 cut(s) 1210, 2109, 2562
BseLI CCNNNNNNNGG 5 cut(s) 341, 1639, 2441, 2497, 2895
BseMI GCAATG 2 cut(s) 159, 2073
BseMII CTCAG 7 cut(s) 62, 149, 326, 372, 1683, 1800, 2150
BseNI ACTGG 4 cut(s) 1207, 1337, 2123, 2205
BseRI GAGGAG 6 cut(s) 158, 300, 893, 1533, 2801, 2875
BseSI GKGCMC 1 cut(s) 668
BseXI GCAGC 4 cut(s) 136, 956, 1688, 2318
BsgI GTGCAG 2 cut(s) 776, 1695
Bsh1236I CGCG 2 cut(s) 40, 131
BshFI GGCC 6 cut(s) 103, 666, 1336, 2477, 2687, 2800
BshNI GGYRCC 2 cut(s) 34, 1600
BshVI ATCGAT 2 cut(s) 1038, 2805
BsiSI CCGG 5 cut(s) 70, 104, 226, 1604, 1958
BslFI GGGAC 5 cut(s) 1299, 1876, 1894, 2444, 2612
BslI CCNNNNNNNGG 5 cut(s) 341, 1639, 2441, 2497, 2895
BsmAI GTCTC 5 cut(s) 152, 310, 1145, 1787, 2128
BsmBI CGTCTC 1 cut(s) 1787
BsmFI GGGAC 5 cut(s) 1299, 1876, 1894, 2444, 2612
BsnI GGCC 6 cut(s) 103, 666, 1336, 2477, 2687, 2800
Bso31I GGTCTC 2 cut(s) 152, 2128
Bsp120I GGGCCC 1 cut(s) 664
Bsp1286I GDGCHC 1 cut(s) 668
Bsp68I TCGCGA 1 cut(s) 131
BspACI CCGC 7 cut(s) 38, 204, 273, 482, 838, 1358, 1774
BspANI GGCC 6 cut(s) 103, 666, 1336, 2477, 2687, 2800
BspCNI CTCAG 7 cut(s) 61, 148, 325, 371, 1684, 1799, 2149
BspDI ATCGAT 2 cut(s) 1038, 2805
BspFNI CGCG 2 cut(s) 40, 131
BspLI GGNNCC 4 cut(s) 36, 666, 1602, 2478
BspMI ACCTGC 1 cut(s) 2897
BspOI GCTAGC 1 cut(s) 2324
BspPI GGATC 6 cut(s) 217, 1043, 1289, 1478, 1865, 2340
BspT107I GGYRCC 2 cut(s) 34, 1600
BspTNI GGTCTC 2 cut(s) 152, 2128
BsrBI CCGCTC 1 cut(s) 275
BsrDI GCAATG 2 cut(s) 159, 2073
BsrI ACTGG 4 cut(s) 1207, 1337, 2123, 2205
BssECI CCNNGG 7 cut(s) 98, 162, 199, 289, 1215, 1547, 2168
BssNI GRCGYC 1 cut(s) 114
BssT1I CCWWGG 3 cut(s) 98, 1547, 2168
Bst2UI CCWGG 5 cut(s) 164, 291, 336, 375, 2326
Bst4CI ACNGT 9 cut(s) 265, 581, 1139, 1374, 1814, 1929, 2042, 2579, 2670
Bst6I CTCTTC 2 cut(s) 24, 744
BstACI GRCGYC 1 cut(s) 114
BstBAI YACGTR 1 cut(s) 1673
BstC8I GCNNGC 6 cut(s) 129, 778, 1017, 2322, 2638, 2924
BstF5I GGATG 8 cut(s) 25, 225, 649, 1318, 1460, 1924, 2334, 2563
BstFNI CGCG 2 cut(s) 40, 131
BstMAI GTCTC 5 cut(s) 152, 310, 1145, 1787, 2128
BstMWI GCNNNNNNNGC 3 cut(s) 100, 2429, 2912
BstNI CCWGG 5 cut(s) 164, 291, 336, 375, 2326
BstNSI RCATGY 1 cut(s) 1019
BstPAI GACNNNNGTC 1 cut(s) 1927
BstSCI CCNGG 7 cut(s) 162, 225, 289, 334, 373, 1957, 2324
BstSFI CTRYAG 3 cut(s) 363, 698, 1948
BstSLI GKGCMC 1 cut(s) 668
BstUI CGCG 2 cut(s) 40, 131
BstV1I GCAGC 4 cut(s) 136, 956, 1688, 2318
BstV2I GAAGAC 2 cut(s) 582, 2580
BstX2I RGATCY 4 cut(s) 472, 1483, 2236, 2245
BstYI RGATCY 4 cut(s) 472, 1483, 2236, 2245
Bsu15I ATCGAT 2 cut(s) 1038, 2805
BsuI GTATCC 2 cut(s) 1172, 2977
BsuRI GGCC 6 cut(s) 103, 666, 1336, 2477, 2687, 2800
BsuTUI ATCGAT 2 cut(s) 1038, 2805
BtrI CACGTC 1 cut(s) 619
BtsCI GGATG 8 cut(s) 25, 225, 649, 1318, 1460, 1924, 2334, 2563
BtsI GCAGTG 1 cut(s) 1402
BtsIMutI CAGTG 2 cut(s) 1379, 1402
BtuMI TCGCGA 1 cut(s) 131
BveI ACCTGC 1 cut(s) 2897
Cac8I GCNNGC 6 cut(s) 129, 778, 1017, 2322, 2638, 2924
Cfr13I GGNCC 5 cut(s) 354, 664, 665, 2063, 2476
ClaI ATCGAT 2 cut(s) 1038, 2805
CseI GACGC 4 cut(s) 29, 116, 122, 618
Csp6I GTAC 5 cut(s) 1601, 1940, 2152, 2671, 2958
CspCI CAANNNNNGTGG 2 cut(s) 850, 885
CviQI GTAC 5 cut(s) 1601, 1940, 2152, 2671, 2958
DraI TTTAAA 1 cut(s) 1887
DraIII CACNNNGTG 1 cut(s) 1374
DrdI GACNNNNNNGTC 1 cut(s) 959
DseDI GACNNNNNNGTC 1 cut(s) 959
EaeI YGGCCR 1 cut(s) 101
Eam1104I CTCTTC 2 cut(s) 24, 744
EarI CTCTTC 2 cut(s) 24, 744
Eco130I CCWWGG 3 cut(s) 98, 1547, 2168
Eco24I GRGCYC 1 cut(s) 668
Eco31I GGTCTC 2 cut(s) 152, 2128
Eco32I GATATC 2 cut(s) 845, 1955
Eco47I GGWCC 2 cut(s) 354, 2063
Eco57I CTGAAG 3 cut(s) 310, 1934, 2253
Eco72I CACGTG 1 cut(s) 1673
EcoO109I RGGNCCY 3 cut(s) 354, 664, 2476
EcoRI GAATTC 1 cut(s) 419
EcoRII CCWGG 5 cut(s) 162, 289, 334, 373, 2324
EcoRV GATATC 2 cut(s) 845, 1955
EcoT14I CCWWGG 3 cut(s) 98, 1547, 2168
EcoT22I ATGCAT 3 cut(s) 566, 1017, 1593
EcoT38I GRGCYC 1 cut(s) 668
ErhI CCWWGG 3 cut(s) 98, 1547, 2168
Esp3I CGTCTC 1 cut(s) 1787
FalI AAGNNNNNCTT 8 cut(s) 810, 842, 905, 937, 1815, 1847, 2152, 2184
FaqI GGGAC 5 cut(s) 1299, 1876, 1894, 2444, 2612
FblI GTMKAC 2 cut(s) 43, 1947
Fnu4HI GCNGC 7 cut(s) 38, 125, 483, 839, 945, 1677, 2307
FokI GGATG 8 cut(s) 32, 232, 656, 1325, 1447, 1931, 2341, 2570
FriOI GRGCYC 1 cut(s) 668
Fsp4HI GCNGC 7 cut(s) 38, 125, 483, 839, 945, 1677, 2307
GluI GCNGC 7 cut(s) 38, 125, 483, 839, 945, 1677, 2307
GsuI CTGGAG 5 cut(s) 185, 318, 396, 474, 1418
HaeIII GGCC 6 cut(s) 103, 666, 1336, 2477, 2687, 2800
HapII CCGG 5 cut(s) 70, 104, 226, 1604, 1958
HgaI GACGC 4 cut(s) 29, 116, 122, 618
Hin1I GRCGYC 1 cut(s) 114
HincII GTYRAC 1 cut(s) 637
HindII GTYRAC 1 cut(s) 637
HindIII AAGCTT 3 cut(s) 1084, 1635, 2178
HpaII CCGG 5 cut(s) 70, 104, 226, 1604, 1958
HphI GGTGA 7 cut(s) 350, 422, 745, 897, 1139, 1715, 2120
Hpy166II GTNNAC 9 cut(s) 44, 85, 413, 428, 637, 1948, 1964, 2066, 2165
Hpy188I TCNGA 8 cut(s) 51, 138, 194, 315, 438, 940, 1216, 2221
Hpy8I GTNNAC 9 cut(s) 44, 85, 413, 428, 637, 1948, 1964, 2066, 2165
Hpy99I CGWCG 2 cut(s) 253, 585
HpyCH4III ACNGT 9 cut(s) 265, 581, 1139, 1374, 1814, 1929, 2042, 2579, 2670
HpyCH4IV ACGT 7 cut(s) 279, 618, 633, 639, 1412, 1672, 2068
HpyF10VI GCNNNNNNNGC 3 cut(s) 100, 2429, 2912
HpySE526I ACGT 7 cut(s) 279, 618, 633, 639, 1412, 1672, 2068
Hsp92I GRCGYC 1 cut(s) 114
KpnI GGTACC 1 cut(s) 1604
LmnI GCTCC 3 cut(s) 72, 699, 1437
Lsp1109I GCAGC 4 cut(s) 136, 956, 1688, 2318
LweI GCATC 8 cut(s) 198, 573, 931, 1636, 2419, 2572, 2623, 2692
MaeII ACGT 7 cut(s) 279, 618, 633, 639, 1412, 1672, 2068
MaeIII GTNAC 5 cut(s) 629, 650, 917, 931, 2357
MbiI CCGCTC 1 cut(s) 275
MflI RGATCY 4 cut(s) 472, 1483, 2236, 2245
MhlI GDGCHC 1 cut(s) 668
MlyI GAGTC 3 cut(s) 127, 155, 2221
MmeI TCCRAC 3 cut(s) 1511, 1898, 2005
Mph1103I ATGCAT 3 cut(s) 566, 1017, 1593
MroXI GAANNNNTTC 2 cut(s) 822, 2247
MslI CAYNNNNRTG 2 cut(s) 1170, 1194
MspA1I CMGCKG 2 cut(s) 1868, 2309
MspI CCGG 5 cut(s) 70, 104, 226, 1604, 1958
MspR9I CCNGG 7 cut(s) 164, 227, 291, 336, 375, 1959, 2326
MvaI CCWGG 5 cut(s) 164, 291, 336, 375, 2326
MvnI CGCG 2 cut(s) 40, 131
MwoI GCNNNNNNNGC 3 cut(s) 100, 2429, 2912
NciI CCSGG 2 cut(s) 227, 1959
NheI GCTAGC 1 cut(s) 2320
NlaIV GGNNCC 4 cut(s) 36, 666, 1602, 2478
NmeAIII GCCGAG 1 cut(s) 180
NmuCI GTSAC 2 cut(s) 931, 2357
NruI TCGCGA 1 cut(s) 131
NsiI ATGCAT 3 cut(s) 566, 1017, 1593
NspI RCATGY 1 cut(s) 1019
PaeI GCATGC 1 cut(s) 1019
PcsI WCGNNNNNNNCGW 1 cut(s) 1562
PdmI GAANNNNTTC 2 cut(s) 822, 2247
PfeI GAWTC 9 cut(s) 602, 823, 1148, 1206, 1453, 1763, 1844, 2015, 2761
PflMI CCANNNNNTGG 2 cut(s) 1639, 2497
PfoI TCCNGGA 1 cut(s) 225
PkrI GCNGC 7 cut(s) 39, 126, 484, 840, 946, 1678, 2308
PleI GAGTC 3 cut(s) 127, 154, 2220
PmaCI CACGTG 1 cut(s) 1673
PmlI CACGTG 1 cut(s) 1673
PpsI GAGTC 3 cut(s) 127, 154, 2220
Ppu21I YACGTR 1 cut(s) 1673
PpuMI RGGWCCY 1 cut(s) 354
PshAI GACNNNNGTC 1 cut(s) 1927
PshBI ATTAAT 2 cut(s) 1733, 2540
PsiI TTATAA 1 cut(s) 2547
Psp1406I AACGTT 1 cut(s) 1412
Psp5II RGGWCCY 1 cut(s) 354
Psp6I CCWGG 5 cut(s) 162, 289, 334, 373, 2324
PspCI CACGTG 1 cut(s) 1673
PspGI CCWGG 5 cut(s) 162, 289, 334, 373, 2324
PspN4I GGNNCC 4 cut(s) 36, 666, 1602, 2478
PspOMI GGGCCC 1 cut(s) 664
PspPI GGNCC 5 cut(s) 354, 664, 665, 2063, 2476
PspPPI RGGWCCY 1 cut(s) 354
PsrI GAACNNNNNNTAC 2 cut(s) 2498, 2530
PsuI RGATCY 4 cut(s) 472, 1483, 2236, 2245
PvuII CAGCTG 2 cut(s) 1868, 2309
RruI TCGCGA 1 cut(s) 131
RsaI GTAC 5 cut(s) 1602, 1941, 2153, 2672, 2959
RsaNI GTAC 5 cut(s) 1601, 1940, 2152, 2671, 2958
RseI CAYNNNNRTG 2 cut(s) 1170, 1194
SatI GCNGC 7 cut(s) 38, 125, 483, 839, 945, 1677, 2307
Sau96I GGNCC 5 cut(s) 354, 664, 665, 2063, 2476
SchI GAGTC 3 cut(s) 127, 155, 2221
ScrFI CCNGG 7 cut(s) 164, 227, 291, 336, 375, 1959, 2326
SduI GDGCHC 1 cut(s) 668
SfaNI GCATC 8 cut(s) 198, 573, 931, 1636, 2419, 2572, 2623, 2692
SfcI CTRYAG 3 cut(s) 363, 698, 1948
SinI GGWCC 2 cut(s) 354, 2063
SmiMI CAYNNNNRTG 2 cut(s) 1170, 1194
SmlI CTYRAG 2 cut(s) 1307, 2125
SmoI CTYRAG 2 cut(s) 1307, 2125
SpeI ACTAGT 1 cut(s) 1942
SphI GCATGC 1 cut(s) 1019
SsiI CCGC 7 cut(s) 38, 204, 273, 482, 838, 1358, 1774
SspI AATATT 1 cut(s) 509
StyD4I CCNGG 7 cut(s) 162, 225, 289, 334, 373, 1957, 2324
StyI CCWWGG 3 cut(s) 98, 1547, 2168
TaaI ACNGT 9 cut(s) 265, 581, 1139, 1374, 1814, 1929, 2042, 2579, 2670
TaiI ACGT 7 cut(s) 282, 621, 636, 642, 1415, 1675, 2071
TatI WGTACW 1 cut(s) 2670
TauI GCSGC 3 cut(s) 40, 485, 841
TfiI GAWTC 9 cut(s) 602, 823, 1148, 1206, 1453, 1763, 1844, 2015, 2761
TscAI CASTG 2 cut(s) 1379, 1402
TseFI GTSAC 2 cut(s) 931, 2357
TseI GCWGC 4 cut(s) 124, 944, 1676, 2306
Tsp45I GTSAC 2 cut(s) 931, 2357
TspGWI ACGGA 3 cut(s) 237, 413, 2518
TspRI CASTG 2 cut(s) 1379, 1402
Van91I CCANNNNNTGG 2 cut(s) 1639, 2497
VpaK11BI GGWCC 2 cut(s) 354, 2063
VspI ATTAAT 2 cut(s) 1733, 2540
XapI RAATTY 4 cut(s) 419, 537, 589, 1610
XbaI TCTAGA 3 cut(s) 1756, 2239, 2425
XceI RCATGY 1 cut(s) 1019
XcmI CCANNNNNNNNNTGG 1 cut(s) 2166
XmaJI CCTAGG 1 cut(s) 1547
XmiI GTMKAC 2 cut(s) 43, 1947
XmnI GAANNNNTTC 2 cut(s) 822, 2247
Zsp2I ATGCAT 3 cut(s) 566, 1017, 1593
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.