Rorug02G0020200
ERF Family

Probably involved in the RNA silencing pathway and required for the generation of small interfering RNAs (siRNAs)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0000505
Physical Location & Seq
Reverse (-)
1633401 .. 1639408
6008 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0020200.1

Sequence Viewer

Length: 1905 bp
ATGTCGTCGGAGATCGAGGTCGTCGAGGACGACGAGGTCCAAACCCGCGGCGATCACGAATCGGCGGCGGCGGCGGCGGTAGCCGGTTCGGTCAATTCGGGCGGCGGAGAAGCCAATGGGATTGGGGAGGAGAGTCTGCGAAACGACGTGTACACGGCGGCGGCGTACGGCGATTTGGAGAAGCTTCAGAGATTGGTGGAGTGTGAGGGCTGCTCGGTTTCCGAGACCGATGGGCTGGGCTACTACGCGCTCCAGTGGGCCGCCCTTAACAACCGAACTGCCGCCGCTCAGTATATTATAGAGCATGGAGGAGATGTGAATGCAAAGGACCATGCGGGGCAGACGGCATTGCACTGGAGTGCAGTGCGGGGTGCTATCCAGGTTGCGGAGCTATTACTCCAAGAGGGTGCGAGGGTGAATGCGGGTGATATGTATGGCTATCAGACTACACATGTTGCGGCCCAATATGGTCAGACTGCTTTCCTATACCATATTGTTTCAAAATGGAATGCTGACCCTGATGTTCCTGATAATGATGGAAGAAGCCCTTTGCACTGGGCTGCTTATAAAGGTTTTGCTGATTGTATACGTCTTCTTTTGTTTCTGGATGCATATAGAGGGCGCCAAGATAAAGAGGGTTGCACTCCTCTACATTGGGCTGCTATCAGGGGTAACTTGGAGGCATGCACAGTTTTGGTACAAGCTGGGAAAAAGGAGGACTTGATGGTTACAGATAACACTGGCCTTACACCAGCACAACTTGCTTCTGATAAAAACCACAGACAAGTTGCTTTTTTCCTTGGGAATGCTCGAAGGCTGCTGGACAAACGTTGTGATGGGAACAGTCGTCTTGGACAACTTTCAAAACTAGGACTTGCACCAGGACTTTGGTGTATAATCTTTGTGCTCCTTGTGACCTATACTCATTCTGTTATAATGGCACCAAATCTGCCAAAGTTAACAGCCGGCTCAGTTTTTATTGCATGGCTTGGAGTTTTCCTAGCAACTGCAGGGCTGGTTATGTTCTATCGGTGTAGCAGTAAAGATCCCGGTTACATCCGAATGAATATCCATGATTCACAAAATATGAAAGATGATGAACCTCTTTTGAAGATTGAGATAAATAATCCAGCCTTGCTAGCTGGTAACTGGTCTCAGCTCTGTGCAACTTGCAAGATTGTCAGGCCTCTTCGCGCAAAGCACTGTTCGACTTGTGACCGTTGTGTTGAACAATTTGACCACCATTGCCCTTGGGTATCCAATTGCATTGGCAAGAAAAACAAATGGGATTTCTTTATTTTCCTTGTTCTTGAAGTTTTGGCAATGTTGACTACTGGAGGAGTCACTCTTACGAGAATTTGGAGTGATCCACTCGGTCCATCTTCATTTGGAGCATGGCTTAATCATGTTGGTACAAGTCATATCGGTGCTATATCATTTTTGATTGTTGATTTCTTCCTCTTCTTCGGTGTGGCCGTTTTGACTGTTGTACAAGCTTCTCAAATCTCGCGAAATATTACAACTAATGAAATGGCAAATGCAATGCGCTATAACTACCTTCGAGGGCCAGGTGGTCGCTTCAGAAACCCATATGATCATGGGATGAAGAAGAATTGCACAGATTTCTTAATCAAGGGCTACAATGAAGATGTAGAATTGATTGAAGAATCAACTCGTGCTGAGGGGACTGGTATGAGGCACATGAATGGGGATTCCCATTCTCACCACACAAATGGCAATGGTCATGTTGCAATCAACGTAAATTCGAATGCATCAGCACATCAAGGTCATGTTCATTCTGCCCATTGCAGCCATAACAATCATGGTAAACCAAAAACGGATAATGTCCCGGTAGGCTTGGGTCTTGGCCTTGGACGGAACGCTTCCCGACCTACTGTATCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RdRP

Protein Analysis

634

Amino Acids

69.2

Weight (kDa)

6.39

Isoelectric Point (pI)

30.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 52 - 142 2.3e-15 Ankyrin repeats (3 copies)
Ank_4 PF13637 86 - 133 7e-07 Ankyrin repeats (many copies)
Ank_2 PF12796 151 - 237 3.4e-16 Ankyrin repeats (3 copies)
Ank_2 PF12796 207 - 267 4.1e-08 Ankyrin repeats (3 copies)
DHHC PF01529 386 - 513 2.2e-32 DHHC palmitoyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000505)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G19910 AT2G19910 AT2G19920 AT2G19920 AT2G19920 AT2G19920 AT2G19930 AT2G19930 AT2G19930
fragaria_vesca FvH4_1g05980 FvH4_1g05990 FvH4_1g05990 FvH4_1g09718
malus_domestica MD02G1064400.v1.1 MD15G1195300.v1.1 MD15G1195400.v1.1
prunus_persica Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1
pyrus_communis pycom02g05070 pycom15g17460
rosa_chinensis RchiOBHm_Chr2g0091671 RchiOBHm_Chr2g0091681 RchiOBHm_Chr2g0091691 RchiOBHm_Chr2g0091701 RchiOBHm_Chr2g0091711 RchiOBHm_Chr2g0091721 RchiOBHm_Chr2g0091731 RchiOBHm_Chr5g0014401
rosa_laevigata RLG00000013772 RLG00000016242 RLG00000016244 RLG00000016245 RLG00000016246 RLG00000032105
rosa_multiflora Rmu_co8374041.1_g000001 Rmu_co8478121.1_g000001 Rmu_sc0000897.1_g000005 Rmu_sc0000897.1_g000009
rosa_roxburghii Rroxscaffold_1G00062180 Rroxscaffold_1G00062190 Rroxscaffold_1G00068800 Rroxscaffold_2G00149790 Rroxscaffold_2G00149800 Rroxscaffold_2G00149810
rosa_rugosa Rorug02G0019600 Rorug02G0019700 Rorug02G0019800 Rorug02G0019900 Rorug02G0019900 Rorug02G0020000 Rorug02G0020000 Rorug02G0020100 Rorug02G0020100 Rorug02G0020200 Rorug05G0017800
rosa_samantha Rh2AG064700 Rh2AG064800 Rh2AG064900 Rh2BG063800 Rh2BG063900 Rh2BG064000 Rh2BG064100 Rh2CG065500 Rh2CG065600 Rh2CG065700 Rh2DG063900 Rh2DG064000 Rh2DG064100 Rh2DG064200 Rh2DG381700 Rh5AG056700 Rh5AG056800 Rh5AG056900 Rh5AG112600 Rh5BG109300 Rh5CG063800 Rh5CG121100 Rh5DG053100 Rh5DG108300 Rh6CG100100
rosa_wichuraiana Rw2G005560 Rw2G005580 Rw2G005590 Rw5G005370 Rw5G009770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 567, 935
AasI GACNNNNNNGTC 1 cut(s) 35
AccB1I GGYRCC 2 cut(s) 621, 940
AccBSI CCGCTC 1 cut(s) 287
AccI GTMKAC 1 cut(s) 586
AccII CGCG 4 cut(s) 48, 248, 1194, 1510
AclI AACGTT 1 cut(s) 829
AclWI GGATC 2 cut(s) 1040, 1361
AcoI YGGCCR 1 cut(s) 1473
AcsI RAATTY 2 cut(s) 1356, 1762
AcuI CTGAAG 2 cut(s) 170, 1564
AcyI GRCGYC 1 cut(s) 622
AfaI GTAC 5 cut(s) 152, 167, 699, 1414, 1491
AfiI CCNNNNNNNGG 1 cut(s) 385
AflIII ACRYGT 2 cut(s) 147, 451
AgsI TTSAA 6 cut(s) 501, 864, 1111, 1229, 1313, 1664
AjiI CACGTC 1 cut(s) 148
AjnI CCWGG 3 cut(s) 378, 880, 1567
AleI CACNNNNGTG 1 cut(s) 357
AluBI AGCT 6 cut(s) 184, 391, 704, 1142, 1159, 1496
AluI AGCT 6 cut(s) 184, 391, 704, 1142, 1159, 1496
Alw21I GWGCWC 1 cut(s) 909
Alw26I GTCTC 2 cut(s) 218, 1158
AlwI GGATC 2 cut(s) 1040, 1361
AoxI GGCC 7 cut(s) 258, 459, 742, 1184, 1473, 1565, 1867
ApeKI GCWGC 5 cut(s) 210, 560, 659, 817, 1809
ApoI RAATTY 2 cut(s) 1356, 1762
AspLEI GCGC 4 cut(s) 250, 624, 1196, 1548
AspS9I GGNCC 6 cut(s) 37, 258, 328, 460, 1376, 1565
AsuC2I CCSGG 2 cut(s) 1050, 1850
AsuHPI GGTGA 3 cut(s) 427, 437, 1715
AsuII TTCGAA 1 cut(s) 1766
AsuNHI GCTAGC 1 cut(s) 1138
AvaII GGWCC 3 cut(s) 37, 328, 1376
BanI GGYRCC 2 cut(s) 621, 940
BauI CACGAG 1 cut(s) 1674
BbsI GAAGAC 1 cut(s) 584
Bbv12I GWGCWC 1 cut(s) 909
BbvCI CCTCAGC 1 cut(s) 1680
BbvI GCAGC 5 cut(s) 197, 547, 646, 804, 1821
BccI CCATC 5 cut(s) 224, 530, 718, 830, 1387
BceAI ACGGC 4 cut(s) 171, 184, 360, 1460
BciT130I CCWGG 3 cut(s) 380, 882, 1569
BciVI GTATCC 1 cut(s) 1267
BclI TGATCA 1 cut(s) 1594
BcnI CCSGG 2 cut(s) 1050, 1850
BcoDI GTCTC 2 cut(s) 218, 1158
BfaI CTAG 3 cut(s) 869, 1001, 1139
BfmI CTRYAG 1 cut(s) 1008
BfoI RGCGCY 1 cut(s) 625
BfuI GTATCC 1 cut(s) 1267
Bme1390I CCNGG 5 cut(s) 380, 882, 1050, 1569, 1850
Bme18I GGWCC 3 cut(s) 37, 328, 1376
BmgBI CACGTC 1 cut(s) 148
BmgT120I GGNCC 6 cut(s) 37, 258, 328, 460, 1376, 1565
BmiI GGNNCC 2 cut(s) 623, 942
BmrFI CCNGG 5 cut(s) 380, 882, 1050, 1569, 1850
BmrI ACTGGG 1 cut(s) 565
BmsI GCATC 2 cut(s) 598, 1781
BmtI GCTAGC 1 cut(s) 1142
BmuI ACTGGG 1 cut(s) 565
BpiI GAAGAC 1 cut(s) 584
BplI GAGNNNNNCTC 2 cut(s) 197, 229
BpmI CTGGAG 3 cut(s) 236, 376, 1356
Bpu10I CCTNAGC 1 cut(s) 1680
Bpu14I TTCGAA 1 cut(s) 1766
BpuMI CCSGG 2 cut(s) 1050, 1850
BsaHI GRCGYC 1 cut(s) 622
BsaI GGTCTC 2 cut(s) 218, 1158
BsaJI CCNNGG 4 cut(s) 46, 799, 1250, 1870
BsaXI ACNNNNNCTCC 2 cut(s) 300, 330
Bsc4I CCNNNNNNNGG 1 cut(s) 385
Bse118I RCCGGY 2 cut(s) 83, 965
Bse1I ACTGG 7 cut(s) 253, 359, 560, 745, 1154, 1339, 1693
Bse3DI GCAATG 6 cut(s) 347, 1243, 1329, 1548, 1744, 1804
BseBI CCWGG 3 cut(s) 380, 882, 1569
BseDI CCNNGG 4 cut(s) 46, 799, 1250, 1870
BseGI GGATG 3 cut(s) 613, 1056, 1608
BseLI CCNNNNNNNGG 1 cut(s) 385
BseMI GCAATG 6 cut(s) 347, 1243, 1329, 1548, 1744, 1804
BseMII CTCAG 4 cut(s) 302, 984, 1169, 1671
BseNI ACTGG 7 cut(s) 253, 359, 560, 745, 1154, 1339, 1693
BseRI GAGGAG 4 cut(s) 143, 324, 636, 1353
BseXI GCAGC 5 cut(s) 197, 547, 646, 804, 1821
BseYI CCCAGC 2 cut(s) 235, 704
BsgI GTGCAG 1 cut(s) 381
Bsh1236I CGCG 4 cut(s) 48, 248, 1194, 1510
BshFI GGCC 7 cut(s) 260, 461, 744, 1186, 1475, 1567, 1869
BshNI GGYRCC 2 cut(s) 621, 940
BsiHKAI GWGCWC 1 cut(s) 909
BsiSI CCGG 4 cut(s) 84, 966, 1050, 1850
BsiWI CGTACG 1 cut(s) 165
BslFI GGGAC 2 cut(s) 1699, 1832
BslI CCNNNNNNNGG 1 cut(s) 385
BsmAI GTCTC 2 cut(s) 218, 1158
BsmFI GGGAC 2 cut(s) 1699, 1832
BsmI GAATGC 5 cut(s) 325, 424, 514, 811, 1774
BsnI GGCC 7 cut(s) 260, 461, 744, 1186, 1475, 1567, 1869
Bso31I GGTCTC 2 cut(s) 218, 1158
Bsp119I TTCGAA 1 cut(s) 1766
Bsp1286I GDGCHC 1 cut(s) 909
Bsp1407I TGTACA 2 cut(s) 150, 1489
Bsp143I GATC 5 cut(s) 12, 52, 1045, 1366, 1594
Bsp68I TCGCGA 1 cut(s) 1510
BspANI GGCC 7 cut(s) 260, 461, 744, 1186, 1475, 1567, 1869
BspCNI CTCAG 4 cut(s) 301, 983, 1168, 1672
BspFNI CGCG 4 cut(s) 48, 248, 1194, 1510
BspLI GGNNCC 2 cut(s) 623, 942
BspMAI CTGCAG 1 cut(s) 1012
BspOI GCTAGC 1 cut(s) 1142
BspPI GGATC 2 cut(s) 1040, 1361
BspT104I TTCGAA 1 cut(s) 1766
BspT107I GGYRCC 2 cut(s) 621, 940
BspTNI GGTCTC 2 cut(s) 218, 1158
BsrBI CCGCTC 1 cut(s) 287
BsrDI GCAATG 6 cut(s) 347, 1243, 1329, 1548, 1744, 1804
BsrFI RCCGGY 2 cut(s) 83, 965
BsrGI TGTACA 2 cut(s) 150, 1489
BsrI ACTGG 7 cut(s) 253, 359, 560, 745, 1154, 1339, 1693
BssAI RCCGGY 2 cut(s) 83, 965
BssECI CCNNGG 4 cut(s) 46, 799, 1250, 1870
BssMI GATC 5 cut(s) 12, 52, 1045, 1366, 1594
BssNAI GTATAC 1 cut(s) 587
BssNI GRCGYC 1 cut(s) 622
BssSI CACGAG 1 cut(s) 1674
BssT1I CCWWGG 3 cut(s) 799, 1250, 1870
Bst1107I GTATAC 1 cut(s) 587
Bst2BI CACGAG 1 cut(s) 1674
Bst2UI CCWGG 3 cut(s) 380, 882, 1569
Bst4CI ACNGT 6 cut(s) 691, 845, 1205, 1220, 1486, 1897
Bst6I CTCTTC 2 cut(s) 1194, 1466
BstACI GRCGYC 1 cut(s) 622
BstAPI GCANNNNNTGC 1 cut(s) 761
BstAUI TGTACA 2 cut(s) 150, 1489
BstBI TTCGAA 1 cut(s) 1766
BstC8I GCNNGC 3 cut(s) 685, 967, 1140
BstDEI CTNAG 5 cut(s) 288, 970, 1155, 1680, 1902
BstDSI CCRYGG 1 cut(s) 46
BstF5I GGATG 3 cut(s) 613, 1056, 1608
BstFNI CGCG 4 cut(s) 48, 248, 1194, 1510
BstH2I RGCGCY 1 cut(s) 625
BstHHI GCGC 4 cut(s) 250, 624, 1196, 1548
BstKTI GATC 5 cut(s) 15, 55, 1048, 1369, 1597
BstMAI GTCTC 2 cut(s) 218, 1158
BstMBI GATC 5 cut(s) 12, 52, 1045, 1366, 1594
BstMWI GCNNNNNNNGC 5 cut(s) 71, 74, 80, 761, 1139
BstNI CCWGG 3 cut(s) 380, 882, 1569
BstNSI RCATGY 2 cut(s) 455, 687
BstSCI CCNGG 5 cut(s) 378, 880, 1048, 1567, 1848
BstSFI CTRYAG 1 cut(s) 1008
BstUI CGCG 4 cut(s) 48, 248, 1194, 1510
BstV1I GCAGC 5 cut(s) 197, 547, 646, 804, 1821
BstV2I GAAGAC 1 cut(s) 584
BstX2I RGATCY 1 cut(s) 1045
BstXI CCANNNNNNTGG 2 cut(s) 888, 1733
BstYI RGATCY 1 cut(s) 1045
BstZ17I GTATAC 1 cut(s) 587
BsuI GTATCC 1 cut(s) 1267
BsuRI GGCC 7 cut(s) 260, 461, 744, 1186, 1475, 1567, 1869
BtgI CCRYGG 1 cut(s) 46
BtrI CACGTC 1 cut(s) 148
BtsCI GGATG 3 cut(s) 613, 1056, 1608
BtsI GCAGTG 1 cut(s) 369
BtsIMutI CAGTG 6 cut(s) 260, 352, 369, 553, 738, 1201
BtuMI TCGCGA 1 cut(s) 1510
Cac8I GCNNGC 3 cut(s) 685, 967, 1140
CfoI GCGC 4 cut(s) 250, 624, 1196, 1548
Cfr10I RCCGGY 2 cut(s) 83, 965
Cfr13I GGNCC 6 cut(s) 37, 258, 328, 460, 1376, 1565
Cfr42I CCGCGG 1 cut(s) 49
Csp6I GTAC 5 cut(s) 151, 166, 698, 1413, 1490
CviQI GTAC 5 cut(s) 151, 166, 698, 1413, 1490
DdeI CTNAG 5 cut(s) 288, 970, 1155, 1680, 1902
DinI GGCGCC 1 cut(s) 623
DpnI GATC 5 cut(s) 14, 54, 1047, 1368, 1596
DpnII GATC 5 cut(s) 12, 52, 1045, 1366, 1594
DrdI GACNNNNNNGTC 1 cut(s) 35
DseDI GACNNNNNNGTC 1 cut(s) 35
EaeI YGGCCR 1 cut(s) 1473
Eam1104I CTCTTC 2 cut(s) 1194, 1466
EarI CTCTTC 2 cut(s) 1194, 1466
EciI GGCGGA 1 cut(s) 120
Eco130I CCWWGG 3 cut(s) 799, 1250, 1870
Eco147I AGGCCT 1 cut(s) 1186
Eco31I GGTCTC 2 cut(s) 218, 1158
Eco47I GGWCC 3 cut(s) 37, 328, 1376
Eco57I CTGAAG 2 cut(s) 170, 1564
EcoRII CCWGG 3 cut(s) 378, 880, 1567
EcoT14I CCWWGG 3 cut(s) 799, 1250, 1870
EcoT22I ATGCAT 2 cut(s) 613, 1774
EgeI GGCGCC 1 cut(s) 623
EheI GGCGCC 1 cut(s) 623
ErhI CCWWGG 3 cut(s) 799, 1250, 1870
FalI AAGNNNNNCTT 4 cut(s) 532, 564, 704, 736
FaqI GGGAC 2 cut(s) 1699, 1832
FauI CCCGC 4 cut(s) 53, 328, 360, 415
FauNDI CATATG 1 cut(s) 1591
FbaI TGATCA 1 cut(s) 1594
FblI GTMKAC 1 cut(s) 586
FokI GGATG 3 cut(s) 620, 1043, 1615
FspBI CTAG 3 cut(s) 869, 1001, 1139
GlaI GCGC 4 cut(s) 249, 623, 1195, 1547
GsaI CCCAGC 2 cut(s) 239, 708
GsuI CTGGAG 3 cut(s) 236, 376, 1356
HaeII RGCGCY 1 cut(s) 625
HaeIII GGCC 7 cut(s) 260, 461, 744, 1186, 1475, 1567, 1869
HapII CCGG 4 cut(s) 84, 966, 1050, 1850
HhaI GCGC 4 cut(s) 250, 624, 1196, 1548
Hin1I GRCGYC 1 cut(s) 622
Hin6I GCGC 4 cut(s) 248, 622, 1194, 1546
HinP1I GCGC 4 cut(s) 248, 622, 1194, 1546
HincII GTYRAC 2 cut(s) 960, 1329
HindII GTYRAC 2 cut(s) 960, 1329
HindIII AAGCTT 2 cut(s) 182, 1494
HinfI GANTC 6 cut(s) 59, 133, 1076, 1341, 1667, 1712
HpaI GTTAAC 1 cut(s) 960
HpaII CCGG 4 cut(s) 84, 966, 1050, 1850
HphI GGTGA 3 cut(s) 427, 437, 1715
Hpy166II GTNNAC 6 cut(s) 151, 153, 587, 960, 1329, 1829
Hpy188I TCNGA 8 cut(s) 10, 189, 223, 444, 474, 769, 1061, 1583
Hpy188III TCNNGA 6 cut(s) 56, 527, 605, 1310, 1509, 1887
Hpy8I GTNNAC 6 cut(s) 151, 153, 587, 960, 1329, 1829
Hpy99I CGWCG 4 cut(s) 10, 26, 35, 149
HpyAV CCTTC 2 cut(s) 807, 1568
HpyCH4III ACNGT 6 cut(s) 691, 845, 1205, 1220, 1486, 1897
HpyCH4IV ACGT 4 cut(s) 147, 589, 829, 1758
HpyF10VI GCNNNNNNNGC 5 cut(s) 71, 74, 80, 761, 1139
HpyF3I CTNAG 5 cut(s) 288, 970, 1155, 1680, 1902
HpySE526I ACGT 4 cut(s) 147, 589, 829, 1758
Hsp92I GRCGYC 1 cut(s) 622
HspAI GCGC 4 cut(s) 248, 622, 1194, 1546
KasI GGCGCC 1 cut(s) 621
KroI GCCGGC 1 cut(s) 965
KroNI GCCGGC 1 cut(s) 967
Ksp22I TGATCA 1 cut(s) 1594
KspAI GTTAAC 1 cut(s) 960
KspI CCGCGG 1 cut(s) 49
Kzo9I GATC 5 cut(s) 12, 52, 1045, 1366, 1594
LmnI GCTCC 4 cut(s) 255, 388, 912, 1391
Lsp1109I GCAGC 5 cut(s) 197, 547, 646, 804, 1821
LweI GCATC 2 cut(s) 598, 1781
MaeI CTAG 3 cut(s) 869, 1001, 1139
MaeII ACGT 4 cut(s) 147, 589, 829, 1758
MaeIII GTNAC 7 cut(s) 671, 727, 913, 1052, 1145, 1214, 1342
MalI GATC 5 cut(s) 14, 54, 1047, 1368, 1596
MbiI CCGCTC 1 cut(s) 287
MboI GATC 5 cut(s) 12, 52, 1045, 1366, 1594
MfeI CAATTG 1 cut(s) 1261
MflI RGATCY 1 cut(s) 1045
MhlI GDGCHC 1 cut(s) 909
MluCI AATT 7 cut(s) 94, 1232, 1261, 1356, 1612, 1655, 1762
Mly113I GGCGCC 1 cut(s) 622
MlyI GAGTC 2 cut(s) 142, 1350
Mph1103I ATGCAT 2 cut(s) 613, 1774
MroNI GCCGGC 1 cut(s) 965
MseI TTAA 4 cut(s) 267, 959, 1401, 1628
MslI CAYNNNNRTG 5 cut(s) 357, 1061, 1425, 1704, 1731
MspA1I CMGCKG 1 cut(s) 48
MspI CCGG 4 cut(s) 84, 966, 1050, 1850
MspR9I CCNGG 5 cut(s) 380, 882, 1050, 1569, 1850
MunI CAATTG 1 cut(s) 1261
Mva1269I GAATGC 5 cut(s) 325, 424, 514, 811, 1774
MvaI CCWGG 3 cut(s) 380, 882, 1569
MvnI CGCG 4 cut(s) 48, 248, 1194, 1510
MwoI GCNNNNNNNGC 5 cut(s) 71, 74, 80, 761, 1139
NaeI GCCGGC 1 cut(s) 967
NarI GGCGCC 1 cut(s) 622
NciI CCSGG 2 cut(s) 1050, 1850
NdeI CATATG 1 cut(s) 1591
NdeII GATC 5 cut(s) 12, 52, 1045, 1366, 1594
NgoMIV GCCGGC 1 cut(s) 965
NheI GCTAGC 1 cut(s) 1138
NlaIV GGNNCC 2 cut(s) 623, 942
NmuCI GTSAC 3 cut(s) 913, 1214, 1342
NruI TCGCGA 1 cut(s) 1510
NsiI ATGCAT 2 cut(s) 613, 1774
NspI RCATGY 2 cut(s) 455, 687
NspV TTCGAA 1 cut(s) 1766
OliI CACNNNNGTG 1 cut(s) 357
PaeI GCATGC 1 cut(s) 687
PceI AGGCCT 1 cut(s) 1186
PciI ACATGT 1 cut(s) 451
PcsI WCGNNNNNNNCGW 5 cut(s) 21, 27, 30, 161, 1473
PctI GAATGC 5 cut(s) 325, 424, 514, 811, 1774
PdiI GCCGGC 1 cut(s) 967
PfeI GAWTC 4 cut(s) 59, 1076, 1667, 1712
Pfl23II CGTACG 1 cut(s) 165
PflFI GACNNNGTC 1 cut(s) 35
PleI GAGTC 2 cut(s) 141, 1349
PluTI GGCGCC 1 cut(s) 625
PpsI GAGTC 2 cut(s) 141, 1349
PscI ACATGT 1 cut(s) 451
PsiI TTATAA 2 cut(s) 567, 935
Psp1406I AACGTT 1 cut(s) 829
Psp6I CCWGG 3 cut(s) 378, 880, 1567
PspFI CCCAGC 2 cut(s) 235, 704
PspGI CCWGG 3 cut(s) 378, 880, 1567
PspLI CGTACG 1 cut(s) 165
PspN4I GGNNCC 2 cut(s) 623, 942
PspPI GGNCC 6 cut(s) 37, 258, 328, 460, 1376, 1565
PstI CTGCAG 1 cut(s) 1012
PsuI RGATCY 1 cut(s) 1045
PsyI GACNNNGTC 1 cut(s) 35
RruI TCGCGA 1 cut(s) 1510
RsaI GTAC 5 cut(s) 152, 167, 699, 1414, 1491
RsaNI GTAC 5 cut(s) 151, 166, 698, 1413, 1490
RseI CAYNNNNRTG 5 cut(s) 357, 1061, 1425, 1704, 1731
SacII CCGCGG 1 cut(s) 49
SaqAI TTAA 4 cut(s) 267, 959, 1401, 1628
Sau3AI GATC 5 cut(s) 12, 52, 1045, 1366, 1594
Sau96I GGNCC 6 cut(s) 37, 258, 328, 460, 1376, 1565
SchI GAGTC 2 cut(s) 142, 1350
ScrFI CCNGG 5 cut(s) 380, 882, 1050, 1569, 1850
SduI GDGCHC 1 cut(s) 909
SfaNI GCATC 2 cut(s) 598, 1781
SfcI CTRYAG 1 cut(s) 1008
SfoI GGCGCC 1 cut(s) 623
Sfr303I CCGCGG 1 cut(s) 49
SfuI TTCGAA 1 cut(s) 1766
SgrBI CCGCGG 1 cut(s) 49
SinI GGWCC 3 cut(s) 37, 328, 1376
SmiMI CAYNNNNRTG 5 cut(s) 357, 1061, 1425, 1704, 1731
SphI GCATGC 1 cut(s) 687
Sse9I AATT 7 cut(s) 94, 1232, 1261, 1356, 1612, 1655, 1762
SseBI AGGCCT 1 cut(s) 1186
SspDI GGCGCC 1 cut(s) 621
SspI AATATT 1 cut(s) 1516
SspMI CTAG 3 cut(s) 869, 1001, 1139
StuI AGGCCT 1 cut(s) 1186
StyD4I CCNGG 5 cut(s) 378, 880, 1048, 1567, 1848
StyI CCWWGG 3 cut(s) 799, 1250, 1870
TaaI ACNGT 6 cut(s) 691, 845, 1205, 1220, 1486, 1897
TaiI ACGT 4 cut(s) 150, 592, 832, 1761
TaqI TCGA 6 cut(s) 15, 24, 811, 1208, 1561, 1766
TaqII GACCGA 3 cut(s) 79, 242, 1364
TasI AATT 7 cut(s) 94, 1232, 1261, 1356, 1612, 1655, 1762
TatI WGTACW 2 cut(s) 150, 1489
TfiI GAWTC 4 cut(s) 59, 1076, 1667, 1712
Tru1I TTAA 4 cut(s) 267, 959, 1401, 1628
Tru9I TTAA 4 cut(s) 267, 959, 1401, 1628
TscAI CASTG 6 cut(s) 260, 359, 369, 560, 745, 1208
TseFI GTSAC 3 cut(s) 913, 1214, 1342
TseI GCWGC 5 cut(s) 210, 560, 659, 817, 1809
Tsp45I GTSAC 3 cut(s) 913, 1214, 1342
TspDTI ATGAA 9 cut(s) 1079, 1103, 1113, 1374, 1542, 1619, 1659, 1718, 1784
TspGWI ACGGA 2 cut(s) 1853, 1891
TspRI CASTG 6 cut(s) 260, 359, 369, 560, 745, 1208
Tth111I GACNNNGTC 1 cut(s) 35
VpaK11BI GGWCC 3 cut(s) 37, 328, 1376
XapI RAATTY 2 cut(s) 1356, 1762
XceI RCATGY 2 cut(s) 455, 687
XcmI CCANNNNNNNNNTGG 1 cut(s) 1820
XmiI GTMKAC 1 cut(s) 586
XspI CTAG 3 cut(s) 869, 1001, 1139
Zsp2I ATGCAT 2 cut(s) 613, 1774
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.