RchiOBHm_Chr5g0014401
ERF Family

Probably involved in the RNA silencing pathway and required for the generation of small interfering RNAs (siRNAs)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000505
Physical Location & Seq
Reverse (-)
9870991 .. 9881391
10401 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ29485

Sequence Viewer

Length: 3471 bp
ATGTTCGGCGTGCAGCTACTCGACTCCGTGCAGCAATCGCTGGATCGAATATGTCACCTTAAGAAGCAACCGCAGCCGGACGACGGCATCAGAAGAAAGCTGGCATTGCTGAGCGAGCAAGACGCCCTCTACGTGCTGGACCAAATTTACAACAGCAAAACTGAGATCAGAACCCTCAATGGATTCATTGCTTTCATGATCAGGAAACTAGAGAATGAATCTTCTTCGTCTCCTTCTTCTAGAACAGTTTGGACCCCTCAGAGTCCCTCTGCTTCTTCTTCTTCTGTTACTAGTCCTCCACAGCTTTATCACAGCCCTGATCATTCTCAAGGTTTGGATTTTTTACTTTCTTTGTCAAGTTGGAAGCAATTTCTGTCTCAATTTGAGTTTGAGGGTGCGGTGGTATTTGCAGTTGTAGACCAGTCTGAAGAGAGTGGACTCTGTTCCTCTGTTTTTCAGCGGCTATATTTGAGCTCTCAAGGTGAGACAATTCCTTGTGGTTCTCCTGGTGTAGAGCAACATCAAGACTATAATCGACAGCCACTCTATTCTTCAGCTTCTCCTTCTGAAGCTTTCCAGACTTCTTATTATCAGAGCCATTCTTCTCCTGCTGGTACTGATGCACCATTTTATTCCAGTTCTCAGCCTCAGGGTGTCCATGTTTCTCCCTTTCAAACCCTATCTGCCTCTCCTGTTACTCCTGTTCAACTTCATAAGAGCTTTCAAGGTGAGACTGTCACTCACTCCCCAGTTCTATACCATCCTGGTGACCATGGACCAGCACACTTAGAAGCTCTGGGAGAACTCGAGTTCAGAAGACAATTTCTAATATTAAGTTATGCTGGAGGAACAAAGTTAAAAGATGTAATAGAAGTGGAGGCTATTAGGCGCTGGAGAAATTTGCCAATGGTAAACTTTGACACCAAAGTTTGGCATGCTTTGGGTGAAAACAATGTTGGCAAGGCTGACCGACGATTGTATTCTGATTGGGATTCTCAAAGAACACACCACTACTATTGTTATGTTTCCTTGGATGGGAGTTGCAAGTTCAAGGGTCCCTTCCTAGACAAACCAAGAACTCTCCTACAGAAATGGTTAGAAGATGACAATGTTTTAGAGGTGAAATTTACCCAAGGGGAAGGTTCCAGTATTCTTTTTGATAATAATTATGGTACATATAGCAAGTTAATGCGAGAAGGCATTCTTGTTGGTTTGCGGCGATATGGTCACTTTGTGCACAGTGTTGGTGGTAACAAAGGAAAGAATAAGTTGAAAGACCCAACTTCATCACCTGTGAAATGTTACTTTGTTCGTATGAGCTCTGATGCTGCGATAGACATGGGTAAATTCTATAAGTTAGCTGGCAAAACGATGCATGAAGGAAGATCTCTTTTGACGCACGTACACACTGGGCCTAGTTTGGCAAATTACATGGCTAGGTATAACAAATTTCTTTCCCGAGCTGTATACAATCAATTCTATTTTCTGATTCTAAATTACCATCCGTGTCCGGGATTCAGGTTATCTCTCATTTTGTCCAAGACAGTTACTTTAGAAGTGAATTGGTCACTTGTCAAGGTTGAGTTCATCAATGAAGAATACTGTCTCGATGAGTCTGGTAATCCCATCTATGTAGATGGAAAAGCTCGTATACATACTGATGGCACAGGATTTATATCTGAGGATCTGGCTTTACTTTGTCCTGAGATAGCACACAAAGGAGAGCTTATCAATAATGAACACATCGAGGGACTTGATGATCCTGATGATGAACTTGAGGACAACATTCTGGAATGGAAACAGCCAGGCACTAGAACTCAGGAACTGCCTTTGCTCATGCAGTTCCGACTTTTTTACAAAGTCTATGCTATCAAAGGAACTTTTCTTGTCAATAAACTGCTTCCACCAAACACATTGCAGATTCGACCGTCAATGGTCAAAGTTGAGCCAGATCCAAAACTGTCAGATCCTCAAACAGTGAATTCACTGGAGATAGTAGGAACAAGTAAACTTACTAAGAGAACAACTTTGTCGAGGAATTTGATTGCACTTTTATACCATGGAGGGGTGCCAAAAGAATACTTCATCGAGTTACTTATGAAAGCTTTAGAAGAGACTCGTGATGTTTATAGCAATAAACGTGCAGCATTCAGAGTTGCCCTTAGATATGGCGAGATCGATGACAATAGTAATGTTGCGAAAATGATCTCCTGTGGTATTCCTCTTGAAGAACCATATATGCAATATCGTCTTTCAGCCCTTATGAACATGGAAAAGATCAATCTTAAAAAAGGGAAAATTTGTATTCCTGAATCATACTATTTGATGGGGACAGCTGATCCAACAGGAAAACTTGAAAAAGATCAAGTTTGCATTATCCTAAAAGATGGACAACTGTCGGGAATGGTTCTAGTGTACCGGAATCCTGGTGTACACTTCGGTGATATTCATGTCCTAAAAGCCACGTATGTCAAGGAACTAGAATCTGTTGTTGGGAATGCCAAGTACGGTATATTCTTCTCTTGTAAAGGTCCACGTTCCATTGCGGATGAAATGGGTGGTGGAGATTTTGATGGTGATCTATACTGGATCTCAAGAAACCCTCAGCTGTTGGAATCGTTTAAACCAAGTGAACCTTGGATTGAAAAGTCATCTACCCAAAAAGTTCATGGTAAAAGACCAACCGAGCTTTCAAATGTTGATCTAGAAGATGAGCTCATCAAACTATTCTTGAAGACTAGGTTTGAACCAAGTGAGGCAGCTCAACACTGGATGGCTTCGATGGATAAATTTTTGAGTTTAGGAGATACGAGTATTGATGAGAAAAACGAACTGAAGGCAAAGATATTGCAATTGATTGATATTTACTATGAAGCTCTCGATGCCCCAAGAAAGGTTGTAGTTCCAAAAGAATTGAAGGTGAAAAAGTTCCCACATTTCATGGAGAAGAAACCCCTAGATTCCTACCATTCAATTTCCATTTTGGGATCAATTTATGATAAGGTGGATGAATATGAAACAGAAAACAGATCTCTCAAAGAAATTCAGAAACTTCCTCTTTTTGAAGTTGACGTTCCTGAGGGTAGCTTGAAAGAATGGAAGAAACTATACCAAGATTACCGGAATGAGATGTCCTCTGCCATGGGAGCCGAAGATAAACAGGGCCAGAATCAGGCAGCCAATATCGTCATAAGAAAGTATAAAGAGATCTTATACAAGGCTGAGGAGTTTGAGCAAAGTGATATGCGGCACAGACAACAAATATATAATGACGCACTCGCTATTTATCACGTTTGTTATGACTATGCCAAGAGTGTTAGGACTGAAACTGGTGCACAAGATGTCAAGAAATGTGGCTTTGCTTGGAAAGTTGCAGGTGCAGCTCTATGCCAGCTTCACGAAGACTGGAGTTTGAAACCTGGTGAAAAGGTTTTCAGAGTCTTACCCTCTGTTTTGAAGGACATTATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RdRP

Protein Analysis

1156

Amino Acids

131.27

Weight (kDa)

6.35

Isoelectric Point (pI)

46.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
4HB_RdRP3_N PF26249 6 - 69 2.7e-07 RdRP3/4/5, N-terminal four helical bundle domain
RdRP_helical PF26252 262 - 326 3e-06 RNA dependent RNA polymerase, helical domain
RdRP PF05183 507 - 1002 6.3e-81 RNA dependent RNA polymerase
RdRP_head PF26253 1052 - 1134 4.2e-09 RdRP, head domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000505)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G19910 AT2G19910 AT2G19920 AT2G19920 AT2G19920 AT2G19920 AT2G19930 AT2G19930 AT2G19930
fragaria_vesca FvH4_1g05980 FvH4_1g05990 FvH4_1g05990 FvH4_1g09718
malus_domestica MD02G1064400.v1.1 MD15G1195300.v1.1 MD15G1195400.v1.1
prunus_persica Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1
pyrus_communis pycom02g05070 pycom15g17460
rosa_chinensis RchiOBHm_Chr2g0091671 RchiOBHm_Chr2g0091681 RchiOBHm_Chr2g0091691 RchiOBHm_Chr2g0091701 RchiOBHm_Chr2g0091711 RchiOBHm_Chr2g0091721 RchiOBHm_Chr2g0091731 RchiOBHm_Chr5g0014401
rosa_laevigata RLG00000013772 RLG00000016242 RLG00000016244 RLG00000016245 RLG00000016246 RLG00000032105
rosa_multiflora Rmu_co8374041.1_g000001 Rmu_co8478121.1_g000001 Rmu_sc0000897.1_g000005 Rmu_sc0000897.1_g000009
rosa_roxburghii Rroxscaffold_1G00062180 Rroxscaffold_1G00062190 Rroxscaffold_1G00068800 Rroxscaffold_2G00149790 Rroxscaffold_2G00149800 Rroxscaffold_2G00149810
rosa_rugosa Rorug02G0019600 Rorug02G0019700 Rorug02G0019800 Rorug02G0019900 Rorug02G0019900 Rorug02G0020000 Rorug02G0020000 Rorug02G0020100 Rorug02G0020100 Rorug02G0020200 Rorug05G0017800
rosa_samantha Rh2AG064700 Rh2AG064800 Rh2AG064900 Rh2BG063800 Rh2BG063900 Rh2BG064000 Rh2BG064100 Rh2CG065500 Rh2CG065600 Rh2CG065700 Rh2DG063900 Rh2DG064000 Rh2DG064100 Rh2DG064200 Rh2DG381700 Rh5AG056700 Rh5AG056800 Rh5AG056900 Rh5AG112600 Rh5BG109300 Rh5CG063800 Rh5CG121100 Rh5DG053100 Rh5DG108300 Rh6CG100100
rosa_wichuraiana Rw2G005560 Rw2G005580 Rw2G005590 Rw5G005370 Rw5G009770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 3365
Acc36I ACCTGC 1 cut(s) 3365
AccB1I GGYRCC 1 cut(s) 2068
AccB7I CCANNNNNTGG 1 cut(s) 930
AccI GTMKAC 3 cut(s) 417, 1467, 1651
AciI CCGC 6 cut(s) 71, 398, 460, 1216, 2546, 3247
AclWI GGATC 8 cut(s) 51, 1692, 1754, 1946, 1961, 2333, 2597, 2995
AcuI CTGAAG 4 cut(s) 447, 537, 588, 2855
AcyI GRCGYC 1 cut(s) 123
AdeI CACNNNGTG 1 cut(s) 1234
AfaI GTAC 6 cut(s) 616, 1174, 1404, 2417, 2433, 2507
AfiI CCNNNNNNNGG 7 cut(s) 83, 930, 1035, 1511, 2065, 2893, 3172
AflII CTTAAG 1 cut(s) 59
AhlI ACTAGT 1 cut(s) 290
AjnI CCWGG 5 cut(s) 505, 763, 1804, 2425, 3418
AjuI GAANNNNNNNTTGG 6 cut(s) 939, 971, 2475, 2507, 2674, 2706
AleI CACNNNNGTG 1 cut(s) 2439
Alw21I GWGCWC 5 cut(s) 476, 1239, 1322, 2718, 3337
Alw26I GTCTC 6 cut(s) 234, 381, 479, 725, 1610, 2108
Alw44I GTGCAC 2 cut(s) 1235, 3333
AlwI GGATC 8 cut(s) 51, 1692, 1754, 1946, 1961, 2333, 2597, 2995
AlwNI CAGNNNCTG 1 cut(s) 1825
Ama87I CYCGRG 2 cut(s) 806, 1458
AoxI GGCC 2 cut(s) 1412, 3163
ApaLI GTGCAC 2 cut(s) 1235, 3333
ApeKI GCWGC 8 cut(s) 13, 31, 73, 1328, 2144, 2759, 3176, 3380
Asp700I GAANNNNTTC 2 cut(s) 1200, 2927
AspLEI GCGC 1 cut(s) 891
AspS9I GGNCC 7 cut(s) 139, 252, 776, 1055, 1412, 2531, 3163
AsuC2I CCSGG 1 cut(s) 1512
AvaI CYCGRG 2 cut(s) 806, 1458
AvaII GGWCC 5 cut(s) 139, 252, 776, 1055, 2531
AxyI CCTNAGG 2 cut(s) 648, 3078
BaeGI GKGCMC 2 cut(s) 1239, 3337
BanI GGYRCC 1 cut(s) 2068
BanII GRGCYC 3 cut(s) 476, 1322, 2718
BarI GAAGNNNNNNTAC 2 cut(s) 3092, 3124
BauI CACGAG 1 cut(s) 2118
BbsI GAAGAC 3 cut(s) 823, 2741, 3408
Bbv12I GWGCWC 5 cut(s) 476, 1239, 1322, 2718, 3337
BbvCI CCTCAGC 2 cut(s) 2604, 3222
BbvI GCAGC 8 cut(s) 25, 43, 85, 1315, 2156, 2771, 3188, 3392
BceAI ACGGC 1 cut(s) 100
BciT130I CCWGG 5 cut(s) 507, 765, 1806, 2427, 3420
BclI TGATCA 2 cut(s) 198, 319
BcnI CCSGG 1 cut(s) 1512
BcoDI GTCTC 6 cut(s) 234, 381, 479, 725, 1610, 2108
BcuI ACTAGT 1 cut(s) 290
BfmI CTRYAG 1 cut(s) 1085
BfoI RGCGCY 1 cut(s) 892
BfrI CTTAAG 1 cut(s) 59
BfuAI ACCTGC 1 cut(s) 3365
BglII AGATCT 3 cut(s) 1385, 3029, 3207
BlpI GCTNAGC 1 cut(s) 110
Bme1390I CCNGG 6 cut(s) 507, 765, 1512, 1806, 2427, 3420
Bme18I GGWCC 5 cut(s) 139, 252, 776, 1055, 2531
BmeT110I CYCGRG 2 cut(s) 806, 1458
BmgT120I GGNCC 7 cut(s) 139, 252, 776, 1055, 1412, 2531, 3163
BmiI GGNNCC 6 cut(s) 254, 1056, 1057, 1144, 2070, 3148
BmrFI CCNGG 6 cut(s) 507, 765, 1512, 1806, 2427, 3420
BmrI ACTGGG 2 cut(s) 743, 1419
BmsI GCATC 5 cut(s) 96, 610, 1315, 1362, 2872
BmuI ACTGGG 2 cut(s) 743, 1419
BoxI GACNNNNGTC 1 cut(s) 2395
BpiI GAAGAC 3 cut(s) 823, 2741, 3408
BplI GAGNNNNNCTC 2 cut(s) 3119, 3151
BpmI CTGGAG 4 cut(s) 864, 913, 2009, 3427
Bpu10I CCTNAGC 2 cut(s) 2604, 3222
Bpu1102I GCTNAGC 1 cut(s) 110
BpuEI CTTGAG 4 cut(s) 312, 462, 1796, 2578
BpuMI CCSGG 1 cut(s) 1512
Bsa29I ATCGAT 1 cut(s) 2178
BsaAI YACGTR 3 cut(s) 133, 1402, 2466
BsaBI GATNNNNATC 2 cut(s) 1675, 2577
BsaHI GRCGYC 1 cut(s) 123
BsaJI CCNNGG 6 cut(s) 772, 1029, 1132, 2059, 2636, 3141
BsaWI WCCGGW 2 cut(s) 2418, 3120
BsaXI ACNNNNNCTCC 2 cut(s) 280, 310
Bsc4I CCNNNNNNNGG 7 cut(s) 83, 930, 1035, 1511, 2065, 2893, 3172
Bse21I CCTNAGG 2 cut(s) 648, 3078
Bse3DI GCAATG 4 cut(s) 104, 186, 1913, 2541
Bse8I GATNNNNATC 2 cut(s) 1675, 2577
BseBI CCWGG 5 cut(s) 507, 765, 1806, 2427, 3420
BseCI ATCGAT 1 cut(s) 2178
BseDI CCNNGG 6 cut(s) 772, 1029, 1132, 2059, 2636, 3141
BseGI GGATG 6 cut(s) 760, 1039, 1501, 2554, 2778, 3013
BseJI GATNNNNATC 2 cut(s) 1675, 2577
BseLI CCNNNNNNNGG 7 cut(s) 83, 930, 1035, 1511, 2065, 2893, 3172
BseMI GCAATG 4 cut(s) 104, 186, 1913, 2541
BseRI GAGGAG 1 cut(s) 3239
BseSI GKGCMC 2 cut(s) 1239, 3337
BseXI GCAGC 8 cut(s) 25, 43, 85, 1315, 2156, 2771, 3188, 3392
BsgI GTGCAG 4 cut(s) 32, 50, 2163, 3399
Bsh1285I CGRYCG 1 cut(s) 1928
BshFI GGCC 2 cut(s) 1414, 3165
BshNI GGYRCC 1 cut(s) 2068
BshVI ATCGAT 1 cut(s) 2178
BsiEI CGRYCG 1 cut(s) 1928
BsiHKAI GWGCWC 5 cut(s) 476, 1239, 1322, 2718, 3337
BsiHKCI CYCGRG 2 cut(s) 806, 1458
BsiSI CCGG 4 cut(s) 77, 1511, 2419, 3121
BslFI GGGAC 4 cut(s) 249, 1041, 1764, 2344
BslI CCNNNNNNNGG 7 cut(s) 83, 930, 1035, 1511, 2065, 2893, 3172
BsmAI GTCTC 6 cut(s) 234, 381, 479, 725, 1610, 2108
BsmBI CGTCTC 1 cut(s) 234
BsmFI GGGAC 4 cut(s) 249, 1041, 1764, 2344
BsmI GAATGC 3 cut(s) 1200, 2147, 2503
BsnI GGCC 2 cut(s) 1414, 3165
BsoBI CYCGRG 2 cut(s) 806, 1458
Bsp1286I GDGCHC 5 cut(s) 476, 1239, 1322, 2718, 3337
Bsp1407I TGTACA 1 cut(s) 2431
Bsp1720I GCTNAGC 1 cut(s) 110
Bsp19I CCATGG 3 cut(s) 772, 2059, 3141
BspACI CCGC 6 cut(s) 71, 398, 460, 1216, 2546, 3247
BspANI GGCC 2 cut(s) 1414, 3165
BspDI ATCGAT 1 cut(s) 2178
BspHI TCATGA 1 cut(s) 195
BspLI GGNNCC 6 cut(s) 254, 1056, 1057, 1144, 2070, 3148
BspMI ACCTGC 1 cut(s) 3365
BspPI GGATC 8 cut(s) 51, 1692, 1754, 1946, 1961, 2333, 2597, 2995
BspT107I GGYRCC 1 cut(s) 2068
BspTI CTTAAG 1 cut(s) 59
BsrDI GCAATG 4 cut(s) 104, 186, 1913, 2541
BsrGI TGTACA 1 cut(s) 2431
BssECI CCNNGG 6 cut(s) 772, 1029, 1132, 2059, 2636, 3141
BssNAI GTATAC 2 cut(s) 1468, 1652
BssNI GRCGYC 1 cut(s) 123
BssSI CACGAG 1 cut(s) 2118
BssT1I CCWWGG 6 cut(s) 772, 1029, 1132, 2059, 2636, 3141
Bst1107I GTATAC 2 cut(s) 1468, 1652
Bst2BI CACGAG 1 cut(s) 2118
Bst2UI CCWGG 5 cut(s) 507, 765, 1806, 2427, 3420
Bst6I CTCTTC 2 cut(s) 423, 2106
BstACI GRCGYC 1 cut(s) 123
BstAFI CTTAAG 1 cut(s) 59
BstAUI TGTACA 1 cut(s) 2431
BstBAI YACGTR 3 cut(s) 133, 1402, 2466
BstC8I GCNNGC 6 cut(s) 11, 102, 116, 936, 1363, 3392
BstDSI CCRYGG 3 cut(s) 772, 2059, 3141
BstEII GGTNACC 1 cut(s) 767
BstF5I GGATG 6 cut(s) 760, 1039, 1501, 2554, 2778, 3013
BstH2I RGCGCY 1 cut(s) 892
BstHHI GCGC 1 cut(s) 891
BstMAI GTCTC 6 cut(s) 234, 381, 479, 725, 1610, 2108
BstMCI CGRYCG 1 cut(s) 1928
BstMWI GCNNNNNNNGC 7 cut(s) 37, 73, 106, 115, 2882, 3146, 3380
BstNI CCWGG 5 cut(s) 507, 765, 1806, 2427, 3420
BstNSI RCATGY 1 cut(s) 938
BstPAI GACNNNNGTC 1 cut(s) 2395
BstPI GGTNACC 1 cut(s) 767
BstSCI CCNGG 6 cut(s) 505, 763, 1510, 1804, 2425, 3418
BstSFI CTRYAG 1 cut(s) 1085
BstSLI GKGCMC 2 cut(s) 1239, 3337
BstV1I GCAGC 8 cut(s) 25, 43, 85, 1315, 2156, 2771, 3188, 3392
BstV2I GAAGAC 3 cut(s) 823, 2741, 3408
BstX2I RGATCY 7 cut(s) 1385, 1684, 1951, 1966, 2589, 3029, 3207
BstYI RGATCY 7 cut(s) 1385, 1684, 1951, 1966, 2589, 3029, 3207
BstZ17I GTATAC 2 cut(s) 1468, 1652
Bsu15I ATCGAT 1 cut(s) 2178
Bsu36I CCTNAGG 2 cut(s) 648, 3078
BsuRI GGCC 2 cut(s) 1414, 3165
BsuTUI ATCGAT 1 cut(s) 2178
BtgI CCRYGG 3 cut(s) 772, 2059, 3141
BtsCI GGATG 6 cut(s) 760, 1039, 1501, 2554, 2778, 3013
BtsIMutI CAGTG 5 cut(s) 1246, 1407, 1983, 1985, 2767
BveI ACCTGC 1 cut(s) 3365
Cac8I GCNNGC 6 cut(s) 11, 102, 116, 936, 1363, 3392
CaiI CAGNNNCTG 1 cut(s) 1825
CciI TCATGA 1 cut(s) 195
CfoI GCGC 1 cut(s) 891
Cfr13I GGNCC 7 cut(s) 139, 252, 776, 1055, 1412, 2531, 3163
ClaI ATCGAT 1 cut(s) 2178
CseI GACGC 3 cut(s) 131, 1405, 3281
CsiI ACCWGGT 1 cut(s) 3418
Csp6I GTAC 6 cut(s) 615, 1173, 1403, 2416, 2432, 2506
CspCI CAANNNNNGTGG 4 cut(s) 998, 1033, 3334, 3369
CviQI GTAC 6 cut(s) 615, 1173, 1403, 2416, 2432, 2506
DraI TTTAAA 1 cut(s) 2623
DraIII CACNNNGTG 1 cut(s) 1234
Eam1104I CTCTTC 2 cut(s) 423, 2106
EarI CTCTTC 2 cut(s) 423, 2106
Ecl136II GAGCTC 3 cut(s) 474, 1320, 2716
Eco130I CCWWGG 6 cut(s) 772, 1029, 1132, 2059, 2636, 3141
Eco24I GRGCYC 3 cut(s) 476, 1322, 2718
Eco47I GGWCC 5 cut(s) 139, 252, 776, 1055, 2531
Eco53kI GAGCTC 3 cut(s) 474, 1320, 2716
Eco57I CTGAAG 4 cut(s) 447, 537, 588, 2855
Eco81I CCTNAGG 2 cut(s) 648, 3078
Eco88I CYCGRG 2 cut(s) 806, 1458
Eco91I GGTNACC 1 cut(s) 767
EcoICRI GAGCTC 3 cut(s) 474, 1320, 2716
EcoO109I RGGNCCY 1 cut(s) 1055
EcoO65I GGTNACC 1 cut(s) 767
EcoRI GAATTC 1 cut(s) 1981
EcoRII CCWGG 5 cut(s) 505, 763, 1804, 2425, 3418
EcoT14I CCWWGG 6 cut(s) 772, 1029, 1132, 2059, 2636, 3141
EcoT22I ATGCAT 1 cut(s) 1377
EcoT38I GRGCYC 3 cut(s) 476, 1322, 2718
ErhI CCWWGG 6 cut(s) 772, 1029, 1132, 2059, 2636, 3141
Esp3I CGTCTC 1 cut(s) 234
FalI AAGNNNNNCTT 8 cut(s) 1043, 1075, 1188, 1220, 1710, 1742, 2620, 2652
FaqI GGGAC 4 cut(s) 249, 1041, 1764, 2344
FbaI TGATCA 2 cut(s) 198, 319
FblI GTMKAC 3 cut(s) 417, 1467, 1651
FokI GGATG 6 cut(s) 747, 1046, 1488, 2561, 2785, 3020
FriOI GRGCYC 3 cut(s) 476, 1322, 2718
GlaI GCGC 1 cut(s) 890
GsuI CTGGAG 4 cut(s) 864, 913, 2009, 3427
HaeII RGCGCY 1 cut(s) 892
HaeIII GGCC 2 cut(s) 1414, 3165
HapII CCGG 4 cut(s) 77, 1511, 2419, 3121
HgaI GACGC 3 cut(s) 131, 1405, 3281
HhaI GCGC 1 cut(s) 891
Hin1I GRCGYC 1 cut(s) 123
Hin6I GCGC 1 cut(s) 889
HinP1I GCGC 1 cut(s) 889
HincII GTYRAC 1 cut(s) 3070
HindII GTYRAC 1 cut(s) 3070
HindIII AAGCTT 2 cut(s) 570, 2103
HpaII CCGG 4 cut(s) 77, 1511, 2419, 3121
Hpy99I CGWCG 2 cut(s) 86, 975
HpyAV CCTTC 9 cut(s) 243, 573, 1069, 1133, 1190, 1373, 2830, 2911, 3451
HpyCH4IV ACGT 7 cut(s) 132, 1401, 2140, 2465, 2536, 3072, 3291
HpyF10VI GCNNNNNNNGC 7 cut(s) 37, 73, 106, 115, 2882, 3146, 3380
HpySE526I ACGT 7 cut(s) 132, 1401, 2140, 2465, 2536, 3072, 3291
Hsp92I GRCGYC 1 cut(s) 123
HspAI GCGC 1 cut(s) 889
KflI GGGWCCC 1 cut(s) 1055
Ksp22I TGATCA 2 cut(s) 198, 319
LmnI GCTCC 1 cut(s) 3146
Lsp1109I GCAGC 8 cut(s) 25, 43, 85, 1315, 2156, 2771, 3188, 3392
LweI GCATC 5 cut(s) 96, 610, 1315, 1362, 2872
MabI ACCWGGT 1 cut(s) 3418
MaeII ACGT 7 cut(s) 132, 1401, 2140, 2465, 2536, 3072, 3291
MfeI CAATTG 1 cut(s) 2852
MflI RGATCY 7 cut(s) 1385, 1684, 1951, 1966, 2589, 3029, 3207
MhlI GDGCHC 5 cut(s) 476, 1239, 1322, 2718, 3337
MlyI GAGTC 6 cut(s) 17, 271, 432, 1622, 2110, 3447
MmeI TCCRAC 4 cut(s) 341, 1870, 2366, 2592
Mph1103I ATGCAT 1 cut(s) 1377
MroXI GAANNNNTTC 2 cut(s) 1200, 2927
MseI TTAA 6 cut(s) 60, 833, 857, 1187, 2286, 2622
MslI CAYNNNNRTG 3 cut(s) 765, 1659, 2439
MspA1I CMGCKG 3 cut(s) 460, 2336, 2608
MspCI CTTAAG 1 cut(s) 59
MspI CCGG 4 cut(s) 77, 1511, 2419, 3121
MspR9I CCNGG 6 cut(s) 507, 765, 1512, 1806, 2427, 3420
MssI GTTTAAAC 1 cut(s) 2623
MunI CAATTG 1 cut(s) 2852
Mva1269I GAATGC 3 cut(s) 1200, 2147, 2503
MvaI CCWGG 5 cut(s) 507, 765, 1806, 2427, 3420
MwoI GCNNNNNNNGC 7 cut(s) 37, 73, 106, 115, 2882, 3146, 3380
NciI CCSGG 1 cut(s) 1512
NcoI CCATGG 3 cut(s) 772, 2059, 3141
NlaIV GGNNCC 6 cut(s) 254, 1056, 1057, 1144, 2070, 3148
NmuCI GTSAC 5 cut(s) 53, 736, 767, 1226, 1566
NsiI ATGCAT 1 cut(s) 1377
NspI RCATGY 1 cut(s) 938
OliI CACNNNNGTG 1 cut(s) 2439
PaeI GCATGC 1 cut(s) 938
PaeR7I CTCGAG 1 cut(s) 806
PagI TCATGA 1 cut(s) 195
PaqCI CACCTGC 1 cut(s) 3365
PcsI WCGNNNNNNNCGW 1 cut(s) 129
PctI GAATGC 3 cut(s) 1200, 2147, 2503
PdmI GAANNNNTTC 2 cut(s) 1200, 2927
PflMI CCANNNNNTGG 1 cut(s) 930
PfoI TCCNGGA 1 cut(s) 1510
PleI GAGTC 6 cut(s) 17, 270, 432, 1621, 2110, 3446
PmeI GTTTAAAC 1 cut(s) 2623
PpsI GAGTC 6 cut(s) 17, 270, 432, 1621, 2110, 3446
Ppu21I YACGTR 3 cut(s) 133, 1402, 2466
PpuMI RGGWCCY 1 cut(s) 1055
PshAI GACNNNNGTC 1 cut(s) 2395
Psp124BI GAGCTC 3 cut(s) 476, 1322, 2718
Psp5II RGGWCCY 1 cut(s) 1055
Psp6I CCWGG 5 cut(s) 505, 763, 1804, 2425, 3418
PspEI GGTNACC 1 cut(s) 767
PspGI CCWGG 5 cut(s) 505, 763, 1804, 2425, 3418
PspN4I GGNNCC 6 cut(s) 254, 1056, 1057, 1144, 2070, 3148
PspPI GGNCC 7 cut(s) 139, 252, 776, 1055, 1412, 2531, 3163
PspPPI RGGWCCY 1 cut(s) 1055
PspXI VCTCGAGB 1 cut(s) 806
PstNI CAGNNNCTG 1 cut(s) 1825
PsuI RGATCY 7 cut(s) 1385, 1684, 1951, 1966, 2589, 3029, 3207
PvuII CAGCTG 2 cut(s) 2336, 2608
RsaI GTAC 6 cut(s) 616, 1174, 1404, 2417, 2433, 2507
RsaNI GTAC 6 cut(s) 615, 1173, 1403, 2416, 2432, 2506
RseI CAYNNNNRTG 3 cut(s) 765, 1659, 2439
SacI GAGCTC 3 cut(s) 476, 1322, 2718
SaqAI TTAA 6 cut(s) 60, 833, 857, 1187, 2286, 2622
Sau96I GGNCC 7 cut(s) 139, 252, 776, 1055, 1412, 2531, 3163
SchI GAGTC 6 cut(s) 17, 271, 432, 1622, 2110, 3447
ScrFI CCNGG 6 cut(s) 507, 765, 1512, 1806, 2427, 3420
SduI GDGCHC 5 cut(s) 476, 1239, 1322, 2718, 3337
SexAI ACCWGGT 1 cut(s) 3418
SfaNI GCATC 5 cut(s) 96, 610, 1315, 1362, 2872
SfcI CTRYAG 1 cut(s) 1085
Sfr274I CTCGAG 1 cut(s) 806
SinI GGWCC 5 cut(s) 139, 252, 776, 1055, 2531
SlaI CTCGAG 1 cut(s) 806
SmiMI CAYNNNNRTG 3 cut(s) 765, 1659, 2439
SmlI CTYRAG 6 cut(s) 59, 327, 477, 806, 1775, 2593
SmoI CTYRAG 6 cut(s) 59, 327, 477, 806, 1775, 2593
SpeI ACTAGT 1 cut(s) 290
SphI GCATGC 1 cut(s) 938
SsiI CCGC 6 cut(s) 71, 398, 460, 1216, 2546, 3247
SspI AATATT 1 cut(s) 831
SstI GAGCTC 3 cut(s) 476, 1322, 2718
StyD4I CCNGG 6 cut(s) 505, 763, 1510, 1804, 2425, 3418
StyI CCWWGG 6 cut(s) 772, 1029, 1132, 2059, 2636, 3141
TaiI ACGT 7 cut(s) 135, 1404, 2143, 2468, 2539, 3075, 3294
TaqII GACCGA 1 cut(s) 984
TatI WGTACW 1 cut(s) 2431
TauI GCSGC 3 cut(s) 463, 1219, 3250
Tru1I TTAA 6 cut(s) 60, 833, 857, 1187, 2286, 2622
Tru9I TTAA 6 cut(s) 60, 833, 857, 1187, 2286, 2622
TscAI CASTG 5 cut(s) 1246, 1414, 1983, 1992, 2774
TseFI GTSAC 5 cut(s) 53, 736, 767, 1226, 1566
TseI GCWGC 8 cut(s) 13, 31, 73, 1328, 2144, 2759, 3176, 3380
Tsp45I GTSAC 5 cut(s) 53, 736, 767, 1226, 1566
TspGWI ACGGA 2 cut(s) 16, 1494
TspRI CASTG 5 cut(s) 1246, 1414, 1983, 1992, 2774
Van91I CCANNNNNTGG 1 cut(s) 930
Vha464I CTTAAG 1 cut(s) 59
VneI GTGCAC 2 cut(s) 1235, 3333
VpaK11BI GGWCC 5 cut(s) 139, 252, 776, 1055, 2531
XbaI TCTAGA 2 cut(s) 239, 2704
XceI RCATGY 1 cut(s) 938
XcmI CCANNNNNNNNNTGG 2 cut(s) 2634, 2666
XhoI CTCGAG 1 cut(s) 806
XmiI GTMKAC 3 cut(s) 417, 1467, 1651
XmnI GAANNNNTTC 2 cut(s) 1200, 2927
Zsp2I ATGCAT 1 cut(s) 1377
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.