Rh2BG064000
ERF Family

Probably involved in the RNA silencing pathway and required for the generation of small interfering RNAs (siRNAs)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0000505
Physical Location & Seq
Forward (+)
4907253 .. 4919498
12246 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG064000.1

Sequence Viewer

Length: 2460 bp
ATGTCCGGTGTGCAGCTACTCGACTCCGTCCAGCAATCGCTGGATCGAATATGCGACCTTAAGAACCAACCACAGCCGGACGACGGCATCAGAAGAAAGCTGGCATTGCTGAGCGAGCAAGACGCCCTCCACGTGCTAAACCGAATTTACAACTACAAAACTCCCATCAGAACCCTCGATGGATTGATCACTTTCATGATCGGGAAACTAGAGAATGGATCTTCTTCATCTTCTTCGAGAACAGTTTGGACCCCTCAGAGTCCATCTGCTTCTTCTTCTTCTGTTAGTAGTCCTGCAAGGCCTCATCATTCTCAAGGTGAGACAATCCAGTGTGGTTCTCCTGTTAATCGACAGCCACTCTATTCTTCAGCTTCTCCTTCTCAAGCTTCCCCGACTTCTTCTTATCAGAGCCATTCCTCTCCTACCATTACTGATGCATCATTTTATTCCAGTTCTCAGCCTCAGGGTGAGACTCCCAACCCATCCCTGGGGACAGTGTGCAATGGAAAATCACCCTATTCCTTCGCTTCTCTCTATCAAATCCTATCTGCTCCTTTTAATACTCTTAGACAACTCTATGAGAGGTTCCGAGGTGAGACTGTCACTCACTCCCCAGTTCTATACCATCCTGGAGACCATGGACCAGCACACTTAGAAGCTCTGGGAGAACTCGATTTCAGAAAGCAATTTTTGATTTTGAGTTATGCTGGAGGAACGAAGTTAAAAGATGTAATAGAAGCGGAGGCTATTAGGAGCTGGAAAAGTTTGCCAATGGTAAAGTTCGAGACCACAGTTTGGCATGCTTTGGGTGAAAACAATGTTGGCAAGGCTGACCGACGATTGGGTCCCTTCCTAGACAAACCAAGAACTCTCCTACAGAAACAGTTAGGAGATGACAATGTTTTGGAGGTGAAATTTACCCAAGGGGAAGGTTCCAGTGGTACATATAGCAAGTTGATACGAGAAGGCATTCTTGTTGGTTTGCGGCGATACAGTTTCTTTGTGTTCCGTGCTGGTAACAAAGGAAAGAATATGTGGAAAGACCCAACTTCAACACCTGTGAAATGTTACTTTGTTCGTGGGAGCTCTGATGCAGTGATAGACAGGGGTATAGGCTATAAGTTATCTGGCAAAACGATGCATGAAGGAAGATCTCTTTTCACGCATGCACACACTGGGCCTAGTTTGGCTAGCTACATGGATAGGTTATCTCTCATTTTGTCCAAGACAGTTACTTTAGAAGTAAATTGGTCACATGTCAAGGTGGAATTCATCAAAGACGAAGACTGTCGCGATGAGTCTGGTAATCCCATCTATGTAGATGGAAAACCTCGTATACATACTGATGGCACAGGATTTATATCTGAGGATCTGGCTTTACTTTGTCCTGAGATAGCACGCAAAGGAGAGCAGATCAATAATGAACACATTGAGGGACTTGATGATCCTGATGATGAACTTGAGGACAAAATTCTGGAATGGAAACAGCCAGGCACTAGAACTCGGGAACTGCCTTTGCTCATGCAGTTCCGACTTTTTTACAAAGGCCGTGCTATCAAAGGAACTTTTCTTGTCAATAAAGAGCTTCCACCAAAAACTTTGAAGATTCGACCATCAATGGTTAAAGTTGAGCCAGATCCAAAATTGTCAGATCCTCAAACAGTGAATTCACTGGAGATAGTAGGAACAAGTAAACTTAATAAGAGAACATATTTGTCGAGGAACTTAATTGCACTTTTGTACCATGGAGGGGTGCCAAAAAAATACTTCATCGAGTTGCTTATGAAAGCTTTAGAAGAGACTCGTGATGTTTTTAGCAATCCACGCGCAGCATTCAGAGTTGCCCTTAGATATGGCGAGATCGATGACAACAGCACTGTTGCGAAAATGATCTCCTGTGGTATTCCTCTTGAAGAACCATATGTGCAATATCGTCTTTCAACCCTTATGAACATGGAAAAGATCAATCTCAAAAAAGGGAAAATTTGTATTCCTGAATCATACTATTTGATGGGGACAACTGATCCAACAGGAAAACTTGAAAAAGATGAAGTTTGCATTATCCTAGAAAATGGACAACTACTGTCGGGAGATTTTCTAGTGTACCGGAATCCTGGTTTACACTTTGGTGATATTCATGTCCTAAAGGCCAGATATGTCAAGGAACTAGAATCTGTTGTTGGCAATGCCAAGTACGGTATATTCTTCTCTAGTAAAGGTCCACGTTCCGTTGCTGATGAAATGGCTGGTGGAGATTTTGATGGTGATCTATACTGGATCTCAAGCAACCCTCAGCTATTAAAATCGTTTAAACCAAGTGAACCTTGGATTGGAAAGTCATCTGCCCAAAAAGTTCATGGTAAAAGACCAACCGAGCTTTCAAAGGATGATCTAGAAGATGAGCTCATCAAACTATTCTTGAAGACTAGGTTTGAACCAAGGTATGCTTTGTATTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RdRP

Protein Analysis

819

Amino Acids

91.69

Weight (kDa)

8.6

Isoelectric Point (pI)

38.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
4HB_RdRP3_N PF26249 6 - 67 4.9e-08 RdRP3/4/5, N-terminal four helical bundle domain
RdRP_helical PF26252 217 - 281 3.2e-08 RNA dependent RNA polymerase, helical domain
RdRP PF05183 289 - 807 7.9e-84 RNA dependent RNA polymerase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000505)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G19910 AT2G19910 AT2G19920 AT2G19920 AT2G19920 AT2G19920 AT2G19930 AT2G19930 AT2G19930
fragaria_vesca FvH4_1g05980 FvH4_1g05990 FvH4_1g05990 FvH4_1g09718
malus_domestica MD02G1064400.v1.1 MD15G1195300.v1.1 MD15G1195400.v1.1
prunus_persica Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1
pyrus_communis pycom02g05070 pycom15g17460
rosa_chinensis RchiOBHm_Chr2g0091671 RchiOBHm_Chr2g0091681 RchiOBHm_Chr2g0091691 RchiOBHm_Chr2g0091701 RchiOBHm_Chr2g0091711 RchiOBHm_Chr2g0091721 RchiOBHm_Chr2g0091731 RchiOBHm_Chr5g0014401
rosa_laevigata RLG00000013772 RLG00000016242 RLG00000016244 RLG00000016245 RLG00000016246 RLG00000032105
rosa_multiflora Rmu_co8374041.1_g000001 Rmu_co8478121.1_g000001 Rmu_sc0000897.1_g000005 Rmu_sc0000897.1_g000009
rosa_roxburghii Rroxscaffold_1G00062180 Rroxscaffold_1G00062190 Rroxscaffold_1G00068800 Rroxscaffold_2G00149790 Rroxscaffold_2G00149800 Rroxscaffold_2G00149810
rosa_rugosa Rorug02G0019600 Rorug02G0019700 Rorug02G0019800 Rorug02G0019900 Rorug02G0019900 Rorug02G0020000 Rorug02G0020000 Rorug02G0020100 Rorug02G0020100 Rorug02G0020200 Rorug05G0017800
rosa_samantha Rh2AG064700 Rh2AG064800 Rh2AG064900 Rh2BG063800 Rh2BG063900 Rh2BG064000 Rh2BG064100 Rh2CG065500 Rh2CG065600 Rh2CG065700 Rh2DG063900 Rh2DG064000 Rh2DG064100 Rh2DG064200 Rh2DG381700 Rh5AG056700 Rh5AG056800 Rh5AG056900 Rh5AG112600 Rh5BG109300 Rh5CG063800 Rh5CG121100 Rh5DG053100 Rh5DG108300 Rh6CG100100
rosa_wichuraiana Rw2G005560 Rw2G005580 Rw2G005590 Rw5G005370 Rw5G009770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 843
AccB1I GGYRCC 1 cut(s) 1753
AccB7I CCANNNNNTGG 1 cut(s) 795
AccI GTMKAC 1 cut(s) 1336
AccII CGCG 2 cut(s) 1293, 1827
AciI CCGC 2 cut(s) 740, 985
AclWI GGATC 8 cut(s) 51, 226, 1377, 1439, 1631, 1646, 2018, 2285
AcsI RAATTY 6 cut(s) 144, 914, 1268, 1470, 1666, 1983
AcuI CTGAAG 1 cut(s) 351
AcvI CACGTG 1 cut(s) 133
AcyI GRCGYC 1 cut(s) 123
AfaI GTAC 4 cut(s) 943, 1742, 2105, 2195
AfiI CCNNNNNNNGG 7 cut(s) 83, 487, 488, 795, 841, 1750, 2330
AflII CTTAAG 1 cut(s) 59
AflIII ACRYGT 1 cut(s) 1255
AgsI TTSAA 8 cut(s) 1053, 1603, 1913, 1941, 2042, 2382, 2422, 2435
AjnI CCWGG 4 cut(s) 486, 628, 1489, 2113
AjuI GAANNNNNNNTTGG 8 cut(s) 804, 836, 2163, 2195, 2313, 2345, 2362, 2394
AleI CACNNNNGTG 1 cut(s) 2127
Alw21I GWGCWC 2 cut(s) 1088, 2406
Alw26I GTCTC 6 cut(s) 314, 464, 590, 627, 779, 1793
AlwI GGATC 8 cut(s) 51, 226, 1377, 1439, 1631, 1646, 2018, 2285
Ama87I CYCGRG 1 cut(s) 1503
AoxI GGCC 4 cut(s) 299, 1178, 1546, 2148
ApeKI GCWGC 2 cut(s) 13, 1829
ApoI RAATTY 6 cut(s) 144, 914, 1268, 1470, 1666, 1983
Asp700I GAANNNNTTC 1 cut(s) 969
AspLEI GCGC 1 cut(s) 1829
AspS9I GGNCC 5 cut(s) 249, 641, 845, 1178, 2219
AsuHPI GGTGA 8 cut(s) 329, 479, 504, 605, 821, 922, 2141, 2276
AsuNHI GCTAGC 1 cut(s) 1190
AvaI CYCGRG 1 cut(s) 1503
AvaII GGWCC 4 cut(s) 249, 641, 845, 2219
AxyI CCTNAGG 1 cut(s) 462
BaeI ACNNNNGTAYC 2 cut(s) 1724, 1757
BanI GGYRCC 1 cut(s) 1753
BanII GRGCYC 2 cut(s) 1088, 2406
BauI CACGAG 1 cut(s) 1803
BbrPI CACGTG 1 cut(s) 133
BbsI GAAGAC 2 cut(s) 1290, 2429
Bbv12I GWGCWC 2 cut(s) 1088, 2406
BbvCI CCTCAGC 1 cut(s) 2292
BbvI GCAGC 2 cut(s) 25, 1841
BceAI ACGGC 2 cut(s) 100, 1533
BciT130I CCWGG 4 cut(s) 488, 630, 1491, 2115
BclI TGATCA 1 cut(s) 186
BcoDI GTCTC 6 cut(s) 314, 464, 590, 627, 779, 1793
BfmI CTRYAG 1 cut(s) 875
BfrI CTTAAG 1 cut(s) 59
BglII AGATCT 1 cut(s) 1151
BisI GCNGC 3 cut(s) 14, 986, 1830
BlpI GCTNAGC 1 cut(s) 110
BlsI GCNGC 3 cut(s) 15, 987, 1831
Bme1390I CCNGG 4 cut(s) 488, 630, 1491, 2115
Bme18I GGWCC 4 cut(s) 249, 641, 845, 2219
BmeT110I CYCGRG 1 cut(s) 1503
BmgT120I GGNCC 5 cut(s) 249, 641, 845, 1178, 2219
BmiI GGNNCC 6 cut(s) 251, 587, 846, 847, 934, 1755
BmrFI CCNGG 4 cut(s) 488, 630, 1491, 2115
BmrI ACTGGG 2 cut(s) 608, 1185
BmsI GCATC 5 cut(s) 96, 424, 446, 1081, 1128
BmtI GCTAGC 1 cut(s) 1194
BmuI ACTGGG 2 cut(s) 608, 1185
BpiI GAAGAC 2 cut(s) 1290, 2429
BpmI CTGGAG 3 cut(s) 651, 729, 1694
Bpu10I CCTNAGC 1 cut(s) 2292
Bpu1102I GCTNAGC 1 cut(s) 110
BpuEI CTTGAG 4 cut(s) 297, 366, 1481, 2266
Bsa29I ATCGAT 1 cut(s) 1863
BsaAI YACGTR 1 cut(s) 133
BsaBI GATNNNNATC 2 cut(s) 1360, 2265
BsaHI GRCGYC 1 cut(s) 123
BsaI GGTCTC 2 cut(s) 627, 779
BsaJI CCNNGG 8 cut(s) 486, 487, 589, 637, 922, 1744, 2324, 2438
BsaWI WCCGGW 2 cut(s) 5, 2106
Bsc4I CCNNNNNNNGG 7 cut(s) 83, 487, 488, 795, 841, 1750, 2330
Bse1I ACTGG 7 cut(s) 328, 450, 614, 936, 1180, 1677, 2279
Bse21I CCTNAGG 1 cut(s) 462
Bse3DI GCAATG 3 cut(s) 104, 508, 2191
Bse8I GATNNNNATC 2 cut(s) 1360, 2265
BseBI CCWGG 4 cut(s) 488, 630, 1491, 2115
BseCI ATCGAT 1 cut(s) 1863
BseDI CCNNGG 8 cut(s) 486, 487, 589, 637, 922, 1744, 2324, 2438
BseGI GGATG 3 cut(s) 482, 625, 2392
BseJI GATNNNNATC 2 cut(s) 1360, 2265
BseLI CCNNNNNNNGG 7 cut(s) 83, 487, 488, 795, 841, 1750, 2330
BseMI GCAATG 3 cut(s) 104, 508, 2191
BseMII CTCAG 7 cut(s) 101, 269, 470, 476, 1356, 1380, 2306
BseNI ACTGG 7 cut(s) 328, 450, 614, 936, 1180, 1677, 2279
BseXI GCAGC 2 cut(s) 25, 1841
BsgI GTGCAG 1 cut(s) 32
Bsh1236I CGCG 2 cut(s) 1293, 1827
BshFI GGCC 4 cut(s) 301, 1180, 1548, 2150
BshNI GGYRCC 1 cut(s) 1753
BshVI ATCGAT 1 cut(s) 1863
BsiHKAI GWGCWC 2 cut(s) 1088, 2406
BsiHKCI CYCGRG 1 cut(s) 1503
BsiSI CCGG 3 cut(s) 6, 77, 2107
BslFI GGGAC 4 cut(s) 505, 831, 1449, 2029
BslI CCNNNNNNNGG 7 cut(s) 83, 487, 488, 795, 841, 1750, 2330
BsmAI GTCTC 6 cut(s) 314, 464, 590, 627, 779, 1793
BsmFI GGGAC 4 cut(s) 505, 831, 1449, 2029
BsmI GAATGC 2 cut(s) 969, 1832
BsnI GGCC 4 cut(s) 301, 1180, 1548, 2150
Bso31I GGTCTC 2 cut(s) 627, 779
BsoBI CYCGRG 1 cut(s) 1503
Bsp1286I GDGCHC 2 cut(s) 1088, 2406
Bsp1720I GCTNAGC 1 cut(s) 110
Bsp19I CCATGG 2 cut(s) 637, 1744
Bsp68I TCGCGA 1 cut(s) 1293
BspACI CCGC 2 cut(s) 740, 985
BspANI GGCC 4 cut(s) 301, 1180, 1548, 2150
BspCNI CTCAG 7 cut(s) 102, 268, 469, 475, 1357, 1381, 2305
BspDI ATCGAT 1 cut(s) 1863
BspFNI CGCG 2 cut(s) 1293, 1827
BspHI TCATGA 1 cut(s) 195
BspLI GGNNCC 6 cut(s) 251, 587, 846, 847, 934, 1755
BspOI GCTAGC 1 cut(s) 1194
BspPI GGATC 8 cut(s) 51, 226, 1377, 1439, 1631, 1646, 2018, 2285
BspT107I GGYRCC 1 cut(s) 1753
BspTI CTTAAG 1 cut(s) 59
BspTNI GGTCTC 2 cut(s) 627, 779
BsrDI GCAATG 3 cut(s) 104, 508, 2191
BsrI ACTGG 7 cut(s) 328, 450, 614, 936, 1180, 1677, 2279
BssECI CCNNGG 8 cut(s) 486, 487, 589, 637, 922, 1744, 2324, 2438
BssNAI GTATAC 1 cut(s) 1337
BssNI GRCGYC 1 cut(s) 123
BssSI CACGAG 1 cut(s) 1803
BssT1I CCWWGG 5 cut(s) 637, 922, 1744, 2324, 2438
Bst1107I GTATAC 1 cut(s) 1337
Bst2BI CACGAG 1 cut(s) 1803
Bst2UI CCWGG 4 cut(s) 488, 630, 1491, 2115
Bst6I CTCTTC 1 cut(s) 1791
BstACI GRCGYC 1 cut(s) 123
BstAFI CTTAAG 1 cut(s) 59
BstBAI YACGTR 1 cut(s) 133
BstC8I GCNNGC 6 cut(s) 102, 116, 801, 1167, 1192, 1399
BstDSI CCRYGG 2 cut(s) 637, 1744
BstF5I GGATG 3 cut(s) 482, 625, 2392
BstFNI CGCG 2 cut(s) 1293, 1827
BstHHI GCGC 1 cut(s) 1829
BstMAI GTCTC 6 cut(s) 314, 464, 590, 627, 779, 1793
BstMWI GCNNNNNNNGC 3 cut(s) 106, 115, 1824
BstNI CCWGG 4 cut(s) 488, 630, 1491, 2115
BstNSI RCATGY 3 cut(s) 803, 1169, 1259
BstSCI CCNGG 4 cut(s) 486, 628, 1489, 2113
BstSFI CTRYAG 1 cut(s) 875
BstUI CGCG 2 cut(s) 1293, 1827
BstV1I GCAGC 2 cut(s) 25, 1841
BstV2I GAAGAC 2 cut(s) 1290, 2429
BstX2I RGATCY 6 cut(s) 218, 1151, 1369, 1636, 1651, 2277
BstYI RGATCY 6 cut(s) 218, 1151, 1369, 1636, 1651, 2277
BstZ17I GTATAC 1 cut(s) 1337
Bsu15I ATCGAT 1 cut(s) 1863
Bsu36I CCTNAGG 1 cut(s) 462
BsuRI GGCC 4 cut(s) 301, 1180, 1548, 2150
BsuTUI ATCGAT 1 cut(s) 1863
BtgI CCRYGG 2 cut(s) 637, 1744
BtgZI GCGATG 1 cut(s) 1308
BtsCI GGATG 3 cut(s) 482, 625, 2392
BtsI GCAGTG 1 cut(s) 1101
BtsIMutI CAGTG 8 cut(s) 335, 501, 943, 1101, 1173, 1668, 1670, 1875
BtuMI TCGCGA 1 cut(s) 1293
Cac8I GCNNGC 6 cut(s) 102, 116, 801, 1167, 1192, 1399
CciI TCATGA 1 cut(s) 195
CfoI GCGC 1 cut(s) 1829
Cfr13I GGNCC 5 cut(s) 249, 641, 845, 1178, 2219
ClaI ATCGAT 1 cut(s) 1863
CseI GACGC 1 cut(s) 131
Csp6I GTAC 4 cut(s) 942, 1741, 2104, 2194
CviQI GTAC 4 cut(s) 942, 1741, 2104, 2194
DraI TTTAAA 1 cut(s) 2311
DrdI GACNNNNNNGTC 1 cut(s) 843
DseDI GACNNNNNNGTC 1 cut(s) 843
Eam1104I CTCTTC 1 cut(s) 1791
EarI CTCTTC 1 cut(s) 1791
Ecl136II GAGCTC 2 cut(s) 1086, 2404
Eco130I CCWWGG 5 cut(s) 637, 922, 1744, 2324, 2438
Eco147I AGGCCT 1 cut(s) 301
Eco24I GRGCYC 2 cut(s) 1088, 2406
Eco31I GGTCTC 2 cut(s) 627, 779
Eco47I GGWCC 4 cut(s) 249, 641, 845, 2219
Eco53kI GAGCTC 2 cut(s) 1086, 2404
Eco57I CTGAAG 1 cut(s) 351
Eco72I CACGTG 1 cut(s) 133
Eco81I CCTNAGG 1 cut(s) 462
Eco88I CYCGRG 1 cut(s) 1503
EcoICRI GAGCTC 2 cut(s) 1086, 2404
EcoO109I RGGNCCY 1 cut(s) 845
EcoRI GAATTC 2 cut(s) 1268, 1666
EcoRII CCWGG 4 cut(s) 486, 628, 1489, 2113
EcoT14I CCWWGG 5 cut(s) 637, 922, 1744, 2324, 2438
EcoT22I ATGCAT 2 cut(s) 439, 1143
EcoT38I GRGCYC 2 cut(s) 1088, 2406
ErhI CCWWGG 5 cut(s) 637, 922, 1744, 2324, 2438
FalI AAGNNNNNCTT 5 cut(s) 957, 989, 2308, 2340, 2431
FaqI GGGAC 4 cut(s) 505, 831, 1449, 2029
FauNDI CATATG 1 cut(s) 1921
FbaI TGATCA 1 cut(s) 186
FblI GTMKAC 1 cut(s) 1336
Fnu4HI GCNGC 3 cut(s) 14, 986, 1830
FokI GGATG 3 cut(s) 469, 612, 2399
FriOI GRGCYC 2 cut(s) 1088, 2406
Fsp4HI GCNGC 3 cut(s) 14, 986, 1830
GlaI GCGC 1 cut(s) 1828
GluI GCNGC 3 cut(s) 14, 986, 1830
GsuI CTGGAG 3 cut(s) 651, 729, 1694
HaeIII GGCC 4 cut(s) 301, 1180, 1548, 2150
HapII CCGG 3 cut(s) 6, 77, 2107
HgaI GACGC 1 cut(s) 131
HhaI GCGC 1 cut(s) 1829
Hin1I GRCGYC 1 cut(s) 123
Hin6I GCGC 1 cut(s) 1827
HinP1I GCGC 1 cut(s) 1827
HindIII AAGCTT 2 cut(s) 384, 1788
HinfI GANTC 9 cut(s) 23, 259, 472, 1298, 1606, 1801, 1997, 2110, 2171
HpaII CCGG 3 cut(s) 6, 77, 2107
HphI GGTGA 8 cut(s) 329, 479, 504, 605, 821, 922, 2141, 2276
Hpy166II GTNNAC 6 cut(s) 1337, 1694, 2104, 2120, 2222, 2321
Hpy8I GTNNAC 6 cut(s) 1337, 1694, 2104, 2120, 2222, 2321
Hpy99I CGWCG 2 cut(s) 86, 840
HpyAV CCTTC 6 cut(s) 387, 532, 859, 923, 959, 1139
HpyCH4IV ACGT 2 cut(s) 132, 2224
HpyF10VI GCNNNNNNNGC 3 cut(s) 106, 115, 1824
HpySE526I ACGT 2 cut(s) 132, 2224
Hsp92I GRCGYC 1 cut(s) 123
HspAI GCGC 1 cut(s) 1827
KflI GGGWCCC 1 cut(s) 845
Ksp22I TGATCA 1 cut(s) 186
LmnI GCTCC 3 cut(s) 556, 753, 1083
Lsp1109I GCAGC 2 cut(s) 25, 1841
LweI GCATC 5 cut(s) 96, 424, 446, 1081, 1128
MaeII ACGT 2 cut(s) 132, 2224
MaeIII GTNAC 5 cut(s) 601, 1016, 1067, 1231, 1251
MflI RGATCY 6 cut(s) 218, 1151, 1369, 1636, 1651, 2277
MhlI GDGCHC 2 cut(s) 1088, 2406
MlyI GAGTC 5 cut(s) 17, 268, 466, 1307, 1795
MmeI TCCRAC 2 cut(s) 1555, 2051
Mph1103I ATGCAT 2 cut(s) 439, 1143
MroXI GAANNNNTTC 1 cut(s) 969
MslI CAYNNNNRTG 3 cut(s) 194, 1344, 2127
MspCI CTTAAG 1 cut(s) 59
MspI CCGG 3 cut(s) 6, 77, 2107
MspR9I CCNGG 4 cut(s) 488, 630, 1491, 2115
MssI GTTTAAAC 1 cut(s) 2311
Mva1269I GAATGC 2 cut(s) 969, 1832
MvaI CCWGG 4 cut(s) 488, 630, 1491, 2115
MvnI CGCG 2 cut(s) 1293, 1827
MwoI GCNNNNNNNGC 3 cut(s) 106, 115, 1824
NcoI CCATGG 2 cut(s) 637, 1744
NdeI CATATG 1 cut(s) 1921
NheI GCTAGC 1 cut(s) 1190
NlaIV GGNNCC 6 cut(s) 251, 587, 846, 847, 934, 1755
NmuCI GTSAC 2 cut(s) 601, 1251
NruI TCGCGA 1 cut(s) 1293
NsiI ATGCAT 2 cut(s) 439, 1143
NspI RCATGY 3 cut(s) 803, 1169, 1259
OliI CACNNNNGTG 1 cut(s) 2127
PaeI GCATGC 2 cut(s) 803, 1169
PagI TCATGA 1 cut(s) 195
PasI CCCWGGG 1 cut(s) 487
PceI AGGCCT 1 cut(s) 301
PciI ACATGT 1 cut(s) 1255
PcsI WCGNNNNNNNCGW 1 cut(s) 129
PctI GAATGC 2 cut(s) 969, 1832
PdmI GAANNNNTTC 1 cut(s) 969
PfeI GAWTC 4 cut(s) 1606, 1997, 2110, 2171
PflFI GACNNNGTC 1 cut(s) 26
PflMI CCANNNNNTGG 1 cut(s) 795
PfoI TCCNGGA 1 cut(s) 628
PkrI GCNGC 3 cut(s) 15, 987, 1831
PleI GAGTC 5 cut(s) 17, 267, 466, 1306, 1795
PmaCI CACGTG 1 cut(s) 133
PmeI GTTTAAAC 1 cut(s) 2311
PmlI CACGTG 1 cut(s) 133
PpsI GAGTC 5 cut(s) 17, 267, 466, 1306, 1795
Ppu21I YACGTR 1 cut(s) 133
PpuMI RGGWCCY 1 cut(s) 845
PscI ACATGT 1 cut(s) 1255
Psp124BI GAGCTC 2 cut(s) 1088, 2406
Psp5II RGGWCCY 1 cut(s) 845
Psp6I CCWGG 4 cut(s) 486, 628, 1489, 2113
PspCI CACGTG 1 cut(s) 133
PspGI CCWGG 4 cut(s) 486, 628, 1489, 2113
PspN4I GGNNCC 6 cut(s) 251, 587, 846, 847, 934, 1755
PspPI GGNCC 5 cut(s) 249, 641, 845, 1178, 2219
PspPPI RGGWCCY 1 cut(s) 845
PsuI RGATCY 6 cut(s) 218, 1151, 1369, 1636, 1651, 2277
PsyI GACNNNGTC 1 cut(s) 26
RruI TCGCGA 1 cut(s) 1293
RsaI GTAC 4 cut(s) 943, 1742, 2105, 2195
RsaNI GTAC 4 cut(s) 942, 1741, 2104, 2194
RseI CAYNNNNRTG 3 cut(s) 194, 1344, 2127
SacI GAGCTC 2 cut(s) 1088, 2406
SatI GCNGC 3 cut(s) 14, 986, 1830
Sau96I GGNCC 5 cut(s) 249, 641, 845, 1178, 2219
SchI GAGTC 5 cut(s) 17, 268, 466, 1307, 1795
ScrFI CCNGG 4 cut(s) 488, 630, 1491, 2115
SduI GDGCHC 2 cut(s) 1088, 2406
SfaNI GCATC 5 cut(s) 96, 424, 446, 1081, 1128
SfcI CTRYAG 1 cut(s) 875
SinI GGWCC 4 cut(s) 249, 641, 845, 2219
SmiMI CAYNNNNRTG 3 cut(s) 194, 1344, 2127
SmlI CTYRAG 5 cut(s) 59, 312, 381, 1460, 2281
SmoI CTYRAG 5 cut(s) 59, 312, 381, 1460, 2281
SphI GCATGC 2 cut(s) 803, 1169
SseBI AGGCCT 1 cut(s) 301
SsiI CCGC 2 cut(s) 740, 985
SstI GAGCTC 2 cut(s) 1088, 2406
StuI AGGCCT 1 cut(s) 301
StyD4I CCNGG 4 cut(s) 486, 628, 1489, 2113
StyI CCWWGG 5 cut(s) 637, 922, 1744, 2324, 2438
TaiI ACGT 2 cut(s) 135, 2227
TaqII GACCGA 1 cut(s) 849
TauI GCSGC 1 cut(s) 988
TfiI GAWTC 4 cut(s) 1606, 1997, 2110, 2171
TscAI CASTG 8 cut(s) 335, 501, 943, 1101, 1180, 1668, 1677, 1882
TseFI GTSAC 2 cut(s) 601, 1251
TseI GCWGC 2 cut(s) 13, 1829
Tsp45I GTSAC 2 cut(s) 601, 1251
TspGWI ACGGA 3 cut(s) 16, 998, 2218
TspRI CASTG 8 cut(s) 335, 501, 943, 1101, 1180, 1668, 1677, 1882
Tth111I GACNNNGTC 1 cut(s) 26
Van91I CCANNNNNTGG 1 cut(s) 795
Vha464I CTTAAG 1 cut(s) 59
VpaK11BI GGWCC 4 cut(s) 249, 641, 845, 2219
XapI RAATTY 6 cut(s) 144, 914, 1268, 1470, 1666, 1983
XbaI TCTAGA 1 cut(s) 2392
XceI RCATGY 3 cut(s) 803, 1169, 1259
XcmI CCANNNNNNNNNTGG 3 cut(s) 484, 2322, 2354
XmiI GTMKAC 1 cut(s) 1336
XmnI GAANNNNTTC 1 cut(s) 969
Zsp2I ATGCAT 2 cut(s) 439, 1143
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.