Rh5CG121100
ERF Family

Probably involved in the RNA silencing pathway and required for the generation of small interfering RNAs (siRNAs)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0000505
Physical Location & Seq
Reverse (-)
10037697 .. 10051720
14024 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG121100.1

Sequence Viewer

Length: 2775 bp
ATGTTCGGCGTGCAGCTACTCGACTCCGTGCAGCAATCGCTGGATCGAATATGTCACCTTAAGAAGCAACCGCAGCCGGACGACGGCATCAGAAGAAAGCTGGCATTGCTGAGCGAGCAAGACGCCCTCTACGTGCTGGACCAAATTTACAACAGCAAAACTGAGATCAGAACCCTCAATGGATTCATTGCTTTCATGATCAGGAAACTAGAGAATGAATCTTCTTCGTCTCCTTCTTCTAGAACAGTTTGGACCCCTCAGAGTCCCTCTGCTTCTTCTTCTTCTGTTACTAGTCCTCCACAGCTTTATCACAGCCCTGATCATTCTCAAGTTGTAGACCAGTCTGAAGAGAGTGGACTCTGTTCCTCTGTTTTTCAGCGGCTATATTTGAGCTCTCAAGGTGAGACAATTCCTTGTGGTTCTCCTGGTGTAGAGCAACATCAAGACTATAATCGACAGCCACTCTATTCTTCAGCTTCTCCTTCTGAAGCTTTCCAGACTTCTTATTATCAGAGCCATTCTTCTCCTGCTGGTACTGATGCACCATTTTATTCCAGTTCTCAGCCTCAGGGTGAGACTGTCACTCACTCCCCAGTTCTATACCATCCTGGTGACCATGGACCAGCACACTTAGAAGCTCTGGGAGAACTCGAGTTCAGAAGACAATTTCTAATATTAAGTTATGCTGGAGGAACAAAGTTAAAAGATGTAATAGAAGTGGAGGCTATTAGGCGCTGGAGAAATTTGCCAATGGTAAACTTTGACACCAAAGTTTGGCATGCTTTGGGTGAAAACAATGTTGGCAAGGCTGACCGACGATTGGGTCCCTTCCTAGACAAACCAAGAACTCTCCTACAGAAATGGTTAGAAGATGACAATGTTTTAGAGGTGAAATTTACCCAAGGGGAAGGTTCCAGTATTCTTTTTGATAATAATTATGGTACATATAGCAAGTTAATGCGAGAAGGCATTCTTGTTGGTTTGCGGCGATATGGTCACTTTGTGCACAGTGTTGGTGGTAACAAAGGAAAGAATAAGTTGAAAGACCCAACTTCATCACCTGTGAAATGTTACTTTGTTCGTATGAGCTCTGATGCTGCGATAGACATGGGTAAATTCTATAAGTTAGCTGGCAAAACGATGCATGAAGGAAGATCTCTTTTGACGCACGTACACACTGGGCCTAGTTTGGCAAATTACATGGCTAGGTTATCTCTCATTTTGTCCAAGACAGTTACTTTAGAAGTGAATTGGTCACTTGTCAAGGTTGAGTTCATCAATGAAGAATACTGTCTCGATGAGTCTGGTAATCCCATCTATGTAGATGGAAAAGCTCGTATACATACTGATGGCACAGGATTTATATCTGAGGATCTGGCTTTACTTTGTCCTGAGATAGCACACAAAGGAGAGCTTATCAATAATGAACACATCGAGGGACTTGATGATCCTGATGATGAACTTGAGGACAACATTCTGGAATGGAAACAGCCAGGCACTAGAACTCAGGAACTGCCTTTGCTCATGCAGTTCCGACTTTTTTACAAAGTCTATGCTATCAAAGGAACTTTTCTTGTCAATAAACTGCTTCCACCAAACACATTGCAGATTCGACCGTCAATGGTCAAAGTTGAGCCAGATCCAAAACTGTCAGATCCTCAAACAGTGAATTCACTGGAGATAGTAGGAACAAGTAAACTTACTAAGAGAACAACTTTGTCGAGGAATTTGATTGCACTTTTATACCATGGAGGGGTGCCAAAAGAATACTTCATCGAGTTACTTATGAAAGCTTTAGAAGAGACTCGTGATGTTTATAGCAATAAACGTGCAGCATTCAGAGTTGCCCTTAGATATGGCGAGATCGATGACAATAGTAATGTTGCGAAAATGATCTCCTGTGGTATTCCTCTTGAAGAACCATATATGCAATATCGTCTTTCAGCCCTTATGAACATGGAAAAGATCAATCTTAAAAAAGGGAAAATTTGTATTCCTGAATCATACTATTTGATGGGGACAGCTGATCCAACAGGAAAACTTGAAAAAGATCAAGTTTGCATTATCCTAAAAGATGGACAACTGTCGGGAATGGTTCTAGTGTACCGGAATCCTGGTGTACACTTCGGTGATATTCATGTCCTAAAAGCCACGTATGTCAAGGAACTAGAATCTGTTGTTGGGAATGCCAAGTACGGTATATTCTTCTCTTGTAAAGGTCCACGTTCCATTGCGGATGAAATGGGTGGTGGAGATTTTGATGGTGATCTATACTGGATCTCAAGAAACCCTCAGCTGTTGGAATCGTTTAAACCAAGTGAACCTTGGATTGAAAAGTCATCTACCCAAAAAGTTCATGGTAAAAGACCAACCGAGCTTTCAAATGTTGATCTAGAAGATGAGCTCATCAAACTATTCTTGAAGACTAGGTTTGAACCAAGCTTCGCAATGAGTGAGGCAGCTCAACACTGGATGGCTTCGATGGATAAATTTTTGAGTTTAGGAGATACGAGTATTGATGAGAAAAACGAACTGAAGGCAAAGATATTGCAATTGATTGATATTTACTATGAAGCTCTCGATGCCCCAAGAAAGGTTGTAGTTCCAAAAGAATTGAAGGTGAAAAAGTTCCCACATTTCATGGAGAAGAAACCCCTAGATTCCTACCATTCAATTTCCATTTTGGGATCAATTTATGATAAGGTGGATGAATATGAAACAGAAAACAGATCTCTCAAAGGTGATAAAAGTTCTCTATTGTTTGCTTGTTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RdRP

Protein Analysis

924

Amino Acids

104.1

Weight (kDa)

6.13

Isoelectric Point (pI)

47.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
4HB_RdRP3_N PF26249 6 - 69 2e-07 RdRP3/4/5, N-terminal four helical bundle domain
RdRP_helical PF26252 210 - 274 2.3e-06 RNA dependent RNA polymerase, helical domain
RdRP PF05183 275 - 902 1.1e-93 RNA dependent RNA polymerase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000505)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G19910 AT2G19910 AT2G19920 AT2G19920 AT2G19920 AT2G19920 AT2G19930 AT2G19930 AT2G19930
fragaria_vesca FvH4_1g05980 FvH4_1g05990 FvH4_1g05990 FvH4_1g09718
malus_domestica MD02G1064400.v1.1 MD15G1195300.v1.1 MD15G1195400.v1.1
prunus_persica Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1
pyrus_communis pycom02g05070 pycom15g17460
rosa_chinensis RchiOBHm_Chr2g0091671 RchiOBHm_Chr2g0091681 RchiOBHm_Chr2g0091691 RchiOBHm_Chr2g0091701 RchiOBHm_Chr2g0091711 RchiOBHm_Chr2g0091721 RchiOBHm_Chr2g0091731 RchiOBHm_Chr5g0014401
rosa_laevigata RLG00000013772 RLG00000016242 RLG00000016244 RLG00000016245 RLG00000016246 RLG00000032105
rosa_multiflora Rmu_co8374041.1_g000001 Rmu_co8478121.1_g000001 Rmu_sc0000897.1_g000005 Rmu_sc0000897.1_g000009
rosa_roxburghii Rroxscaffold_1G00062180 Rroxscaffold_1G00062190 Rroxscaffold_1G00068800 Rroxscaffold_2G00149790 Rroxscaffold_2G00149800 Rroxscaffold_2G00149810
rosa_rugosa Rorug02G0019600 Rorug02G0019700 Rorug02G0019800 Rorug02G0019900 Rorug02G0019900 Rorug02G0020000 Rorug02G0020000 Rorug02G0020100 Rorug02G0020100 Rorug02G0020200 Rorug05G0017800
rosa_samantha Rh2AG064700 Rh2AG064800 Rh2AG064900 Rh2BG063800 Rh2BG063900 Rh2BG064000 Rh2BG064100 Rh2CG065500 Rh2CG065600 Rh2CG065700 Rh2DG063900 Rh2DG064000 Rh2DG064100 Rh2DG064200 Rh2DG381700 Rh5AG056700 Rh5AG056800 Rh5AG056900 Rh5AG112600 Rh5BG109300 Rh5CG063800 Rh5CG121100 Rh5DG053100 Rh5DG108300 Rh6CG100100
rosa_wichuraiana Rw2G005560 Rw2G005580 Rw2G005590 Rw5G005370 Rw5G009770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 822
AccB1I GGYRCC 1 cut(s) 1756
AccB7I CCANNNNNTGG 1 cut(s) 774
AccI GTMKAC 2 cut(s) 336, 1339
AciI CCGC 4 cut(s) 71, 379, 985, 2234
AclWI GGATC 8 cut(s) 51, 1380, 1442, 1634, 1649, 2021, 2285, 2695
AcsI RAATTY 8 cut(s) 144, 742, 893, 1115, 1669, 1726, 1986, 2489
AcuI CTGAAG 4 cut(s) 366, 456, 507, 2555
AcyI GRCGYC 1 cut(s) 123
AdeI CACNNNGTG 1 cut(s) 1003
AfaI GTAC 6 cut(s) 535, 943, 1173, 2105, 2121, 2195
AfiI CCNNNNNNNGG 5 cut(s) 83, 774, 820, 1753, 2593
AflII CTTAAG 1 cut(s) 59
AgsI TTSAA 9 cut(s) 1042, 1916, 2045, 2333, 2382, 2422, 2435, 2617, 2673
AhlI ACTAGT 1 cut(s) 290
AjnI CCWGG 4 cut(s) 424, 607, 1492, 2113
AjuI GAANNNNNNNTTGG 6 cut(s) 783, 815, 2163, 2195, 2362, 2394
AleI CACNNNNGTG 1 cut(s) 2127
Alw21I GWGCWC 4 cut(s) 395, 1008, 1091, 2406
Alw26I GTCTC 5 cut(s) 234, 398, 569, 1298, 1796
Alw44I GTGCAC 1 cut(s) 1004
AlwI GGATC 8 cut(s) 51, 1380, 1442, 1634, 1649, 2021, 2285, 2695
AlwNI CAGNNNCTG 1 cut(s) 1513
Ama87I CYCGRG 1 cut(s) 650
AoxI GGCC 1 cut(s) 1181
ApaLI GTGCAC 1 cut(s) 1004
ApeKI GCWGC 6 cut(s) 13, 31, 73, 1097, 1832, 2459
ApoI RAATTY 8 cut(s) 144, 742, 893, 1115, 1669, 1726, 1986, 2489
Asp700I GAANNNNTTC 2 cut(s) 969, 2627
AspLEI GCGC 1 cut(s) 735
AspS9I GGNCC 6 cut(s) 139, 252, 620, 824, 1181, 2219
AvaI CYCGRG 1 cut(s) 650
AvaII GGWCC 5 cut(s) 139, 252, 620, 824, 2219
AxyI CCTNAGG 1 cut(s) 567
BaeGI GKGCMC 1 cut(s) 1008
BanI GGYRCC 1 cut(s) 1756
BanII GRGCYC 3 cut(s) 395, 1091, 2406
BauI CACGAG 1 cut(s) 1806
BbsI GAAGAC 2 cut(s) 667, 2429
Bbv12I GWGCWC 4 cut(s) 395, 1008, 1091, 2406
BbvCI CCTCAGC 1 cut(s) 2292
BbvI GCAGC 6 cut(s) 25, 43, 85, 1084, 1844, 2471
BccI CCATC 9 cut(s) 612, 1319, 1322, 1343, 2008, 2069, 2255, 2467, 2476
BceAI ACGGC 1 cut(s) 100
BciT130I CCWGG 4 cut(s) 426, 609, 1494, 2115
BclI TGATCA 2 cut(s) 198, 319
BcoDI GTCTC 5 cut(s) 234, 398, 569, 1298, 1796
BcuI ACTAGT 1 cut(s) 290
BfmI CTRYAG 1 cut(s) 854
BfoI RGCGCY 1 cut(s) 736
BfrI CTTAAG 1 cut(s) 59
BglII AGATCT 2 cut(s) 1154, 2729
BisI GCNGC 8 cut(s) 14, 32, 74, 380, 986, 1098, 1833, 2460
BlpI GCTNAGC 1 cut(s) 110
BlsI GCNGC 8 cut(s) 15, 33, 75, 381, 987, 1099, 1834, 2461
Bme1390I CCNGG 4 cut(s) 426, 609, 1494, 2115
Bme18I GGWCC 5 cut(s) 139, 252, 620, 824, 2219
BmeT110I CYCGRG 1 cut(s) 650
BmgT120I GGNCC 6 cut(s) 139, 252, 620, 824, 1181, 2219
BmiI GGNNCC 5 cut(s) 254, 825, 826, 913, 1758
BmrFI CCNGG 4 cut(s) 426, 609, 1494, 2115
BmrI ACTGGG 2 cut(s) 587, 1188
BmsI GCATC 5 cut(s) 96, 529, 1084, 1131, 2572
BmuI ACTGGG 2 cut(s) 587, 1188
BoxI GACNNNNGTC 1 cut(s) 2083
BpiI GAAGAC 2 cut(s) 667, 2429
BpmI CTGGAG 3 cut(s) 708, 757, 1697
Bpu10I CCTNAGC 1 cut(s) 2292
Bpu1102I GCTNAGC 1 cut(s) 110
BpuEI CTTGAG 4 cut(s) 312, 381, 1484, 2266
Bsa29I ATCGAT 1 cut(s) 1866
BsaAI YACGTR 3 cut(s) 133, 1171, 2154
BsaBI GATNNNNATC 2 cut(s) 1363, 2265
BsaHI GRCGYC 1 cut(s) 123
BsaJI CCNNGG 4 cut(s) 616, 901, 1747, 2324
BsaWI WCCGGW 1 cut(s) 2106
BsaXI ACNNNNNCTCC 2 cut(s) 280, 310
Bsc4I CCNNNNNNNGG 5 cut(s) 83, 774, 820, 1753, 2593
Bse1I ACTGG 8 cut(s) 340, 555, 593, 915, 1183, 1680, 2279, 2474
Bse21I CCTNAGG 1 cut(s) 567
Bse3DI GCAATG 5 cut(s) 104, 186, 1601, 2229, 2454
Bse8I GATNNNNATC 2 cut(s) 1363, 2265
BseBI CCWGG 4 cut(s) 426, 609, 1494, 2115
BseCI ATCGAT 1 cut(s) 1866
BseDI CCNNGG 4 cut(s) 616, 901, 1747, 2324
BseGI GGATG 4 cut(s) 604, 2242, 2478, 2713
BseJI GATNNNNATC 2 cut(s) 1363, 2265
BseLI CCNNNNNNNGG 5 cut(s) 83, 774, 820, 1753, 2593
BseMI GCAATG 5 cut(s) 104, 186, 1601, 2229, 2454
BseMII CTCAG 9 cut(s) 101, 153, 272, 575, 581, 1359, 1383, 1520, 2306
BseNI ACTGG 8 cut(s) 340, 555, 593, 915, 1183, 1680, 2279, 2474
BseSI GKGCMC 1 cut(s) 1008
BseXI GCAGC 6 cut(s) 25, 43, 85, 1084, 1844, 2471
BsgI GTGCAG 3 cut(s) 32, 50, 1851
Bsh1285I CGRYCG 1 cut(s) 1616
BshFI GGCC 1 cut(s) 1183
BshNI GGYRCC 1 cut(s) 1756
BshVI ATCGAT 1 cut(s) 1866
BsiEI CGRYCG 1 cut(s) 1616
BsiHKAI GWGCWC 4 cut(s) 395, 1008, 1091, 2406
BsiHKCI CYCGRG 1 cut(s) 650
BsiSI CCGG 2 cut(s) 77, 2107
BslFI GGGAC 4 cut(s) 249, 810, 1452, 2032
BslI CCNNNNNNNGG 5 cut(s) 83, 774, 820, 1753, 2593
BsmAI GTCTC 5 cut(s) 234, 398, 569, 1298, 1796
BsmBI CGTCTC 1 cut(s) 234
BsmFI GGGAC 4 cut(s) 249, 810, 1452, 2032
BsmI GAATGC 3 cut(s) 969, 1835, 2191
BsnI GGCC 1 cut(s) 1183
BsoBI CYCGRG 1 cut(s) 650
Bsp1286I GDGCHC 4 cut(s) 395, 1008, 1091, 2406
Bsp1407I TGTACA 1 cut(s) 2119
Bsp1720I GCTNAGC 1 cut(s) 110
Bsp19I CCATGG 2 cut(s) 616, 1747
BspACI CCGC 4 cut(s) 71, 379, 985, 2234
BspANI GGCC 1 cut(s) 1183
BspCNI CTCAG 9 cut(s) 102, 154, 271, 574, 580, 1360, 1384, 1519, 2305
BspDI ATCGAT 1 cut(s) 1866
BspHI TCATGA 1 cut(s) 195
BspLI GGNNCC 5 cut(s) 254, 825, 826, 913, 1758
BspPI GGATC 8 cut(s) 51, 1380, 1442, 1634, 1649, 2021, 2285, 2695
BspT107I GGYRCC 1 cut(s) 1756
BspTI CTTAAG 1 cut(s) 59
BsrDI GCAATG 5 cut(s) 104, 186, 1601, 2229, 2454
BsrGI TGTACA 1 cut(s) 2119
BsrI ACTGG 8 cut(s) 340, 555, 593, 915, 1183, 1680, 2279, 2474
BssECI CCNNGG 4 cut(s) 616, 901, 1747, 2324
BssNAI GTATAC 1 cut(s) 1340
BssNI GRCGYC 1 cut(s) 123
BssSI CACGAG 1 cut(s) 1806
BssT1I CCWWGG 4 cut(s) 616, 901, 1747, 2324
Bst1107I GTATAC 1 cut(s) 1340
Bst2BI CACGAG 1 cut(s) 1806
Bst2UI CCWGG 4 cut(s) 426, 609, 1494, 2115
Bst6I CTCTTC 2 cut(s) 342, 1794
BstACI GRCGYC 1 cut(s) 123
BstAFI CTTAAG 1 cut(s) 59
BstAUI TGTACA 1 cut(s) 2119
BstBAI YACGTR 3 cut(s) 133, 1171, 2154
BstC8I GCNNGC 5 cut(s) 11, 102, 116, 780, 1132
BstDSI CCRYGG 2 cut(s) 616, 1747
BstEII GGTNACC 1 cut(s) 611
BstF5I GGATG 4 cut(s) 604, 2242, 2478, 2713
BstH2I RGCGCY 1 cut(s) 736
BstHHI GCGC 1 cut(s) 735
BstMAI GTCTC 5 cut(s) 234, 398, 569, 1298, 1796
BstMCI CGRYCG 1 cut(s) 1616
BstMWI GCNNNNNNNGC 5 cut(s) 37, 73, 106, 115, 2582
BstNI CCWGG 4 cut(s) 426, 609, 1494, 2115
BstNSI RCATGY 1 cut(s) 782
BstPAI GACNNNNGTC 1 cut(s) 2083
BstPI GGTNACC 1 cut(s) 611
BstSCI CCNGG 4 cut(s) 424, 607, 1492, 2113
BstSFI CTRYAG 1 cut(s) 854
BstSLI GKGCMC 1 cut(s) 1008
BstV1I GCAGC 6 cut(s) 25, 43, 85, 1084, 1844, 2471
BstV2I GAAGAC 2 cut(s) 667, 2429
BstX2I RGATCY 6 cut(s) 1154, 1372, 1639, 1654, 2277, 2729
BstYI RGATCY 6 cut(s) 1154, 1372, 1639, 1654, 2277, 2729
BstZ17I GTATAC 1 cut(s) 1340
Bsu15I ATCGAT 1 cut(s) 1866
Bsu36I CCTNAGG 1 cut(s) 567
BsuRI GGCC 1 cut(s) 1183
BsuTUI ATCGAT 1 cut(s) 1866
BtgI CCRYGG 2 cut(s) 616, 1747
BtsCI GGATG 4 cut(s) 604, 2242, 2478, 2713
BtsIMutI CAGTG 5 cut(s) 1015, 1176, 1671, 1673, 2467
Cac8I GCNNGC 5 cut(s) 11, 102, 116, 780, 1132
CaiI CAGNNNCTG 1 cut(s) 1513
CciI TCATGA 1 cut(s) 195
CfoI GCGC 1 cut(s) 735
Cfr13I GGNCC 6 cut(s) 139, 252, 620, 824, 1181, 2219
ClaI ATCGAT 1 cut(s) 1866
CseI GACGC 2 cut(s) 131, 1174
Csp6I GTAC 6 cut(s) 534, 942, 1172, 2104, 2120, 2194
CviQI GTAC 6 cut(s) 534, 942, 1172, 2104, 2120, 2194
DraI TTTAAA 1 cut(s) 2311
DraIII CACNNNGTG 1 cut(s) 1003
DrdI GACNNNNNNGTC 1 cut(s) 822
DseDI GACNNNNNNGTC 1 cut(s) 822
Eam1104I CTCTTC 2 cut(s) 342, 1794
EarI CTCTTC 2 cut(s) 342, 1794
Ecl136II GAGCTC 3 cut(s) 393, 1089, 2404
Eco130I CCWWGG 4 cut(s) 616, 901, 1747, 2324
Eco24I GRGCYC 3 cut(s) 395, 1091, 2406
Eco47I GGWCC 5 cut(s) 139, 252, 620, 824, 2219
Eco53kI GAGCTC 3 cut(s) 393, 1089, 2404
Eco57I CTGAAG 4 cut(s) 366, 456, 507, 2555
Eco81I CCTNAGG 1 cut(s) 567
Eco88I CYCGRG 1 cut(s) 650
Eco91I GGTNACC 1 cut(s) 611
EcoICRI GAGCTC 3 cut(s) 393, 1089, 2404
EcoO109I RGGNCCY 1 cut(s) 824
EcoO65I GGTNACC 1 cut(s) 611
EcoRI GAATTC 1 cut(s) 1669
EcoRII CCWGG 4 cut(s) 424, 607, 1492, 2113
EcoT14I CCWWGG 4 cut(s) 616, 901, 1747, 2324
EcoT22I ATGCAT 1 cut(s) 1146
EcoT38I GRGCYC 3 cut(s) 395, 1091, 2406
ErhI CCWWGG 4 cut(s) 616, 901, 1747, 2324
Esp3I CGTCTC 1 cut(s) 234
FalI AAGNNNNNCTT 6 cut(s) 957, 989, 1398, 1430, 2308, 2340
FaqI GGGAC 4 cut(s) 249, 810, 1452, 2032
FbaI TGATCA 2 cut(s) 198, 319
FblI GTMKAC 2 cut(s) 336, 1339
Fnu4HI GCNGC 8 cut(s) 14, 32, 74, 380, 986, 1098, 1833, 2460
FokI GGATG 4 cut(s) 591, 2249, 2485, 2720
FriOI GRGCYC 3 cut(s) 395, 1091, 2406
Fsp4HI GCNGC 8 cut(s) 14, 32, 74, 380, 986, 1098, 1833, 2460
GlaI GCGC 1 cut(s) 734
GluI GCNGC 8 cut(s) 14, 32, 74, 380, 986, 1098, 1833, 2460
GsuI CTGGAG 3 cut(s) 708, 757, 1697
HaeII RGCGCY 1 cut(s) 736
HaeIII GGCC 1 cut(s) 1183
HapII CCGG 2 cut(s) 77, 2107
HgaI GACGC 2 cut(s) 131, 1174
HhaI GCGC 1 cut(s) 735
Hin1I GRCGYC 1 cut(s) 123
Hin6I GCGC 1 cut(s) 733
HinP1I GCGC 1 cut(s) 733
HindIII AAGCTT 3 cut(s) 489, 1791, 2440
HpaII CCGG 2 cut(s) 77, 2107
Hpy99I CGWCG 2 cut(s) 86, 819
HpyAV CCTTC 8 cut(s) 243, 492, 838, 902, 959, 1142, 2530, 2611
HpyCH4IV ACGT 5 cut(s) 132, 1170, 1828, 2153, 2224
HpyF10VI GCNNNNNNNGC 5 cut(s) 37, 73, 106, 115, 2582
HpySE526I ACGT 5 cut(s) 132, 1170, 1828, 2153, 2224
Hsp92I GRCGYC 1 cut(s) 123
HspAI GCGC 1 cut(s) 733
KflI GGGWCCC 1 cut(s) 824
Ksp22I TGATCA 2 cut(s) 198, 319
Lsp1109I GCAGC 6 cut(s) 25, 43, 85, 1084, 1844, 2471
LweI GCATC 5 cut(s) 96, 529, 1084, 1131, 2572
MaeII ACGT 5 cut(s) 132, 1170, 1828, 2153, 2224
MfeI CAATTG 1 cut(s) 2552
MflI RGATCY 6 cut(s) 1154, 1372, 1639, 1654, 2277, 2729
MhlI GDGCHC 4 cut(s) 395, 1008, 1091, 2406
MlyI GAGTC 5 cut(s) 17, 271, 351, 1310, 1798
MmeI TCCRAC 3 cut(s) 1558, 2054, 2280
Mph1103I ATGCAT 1 cut(s) 1146
MroXI GAANNNNTTC 2 cut(s) 969, 2627
MseI TTAA 7 cut(s) 60, 677, 701, 956, 1974, 2310, 2773
MslI CAYNNNNRTG 3 cut(s) 609, 1347, 2127
MspA1I CMGCKG 3 cut(s) 379, 2024, 2296
MspCI CTTAAG 1 cut(s) 59
MspI CCGG 2 cut(s) 77, 2107
MspR9I CCNGG 4 cut(s) 426, 609, 1494, 2115
MssI GTTTAAAC 1 cut(s) 2311
MunI CAATTG 1 cut(s) 2552
Mva1269I GAATGC 3 cut(s) 969, 1835, 2191
MvaI CCWGG 4 cut(s) 426, 609, 1494, 2115
MwoI GCNNNNNNNGC 5 cut(s) 37, 73, 106, 115, 2582
NcoI CCATGG 2 cut(s) 616, 1747
NlaIV GGNNCC 5 cut(s) 254, 825, 826, 913, 1758
NmuCI GTSAC 5 cut(s) 53, 580, 611, 995, 1254
NsiI ATGCAT 1 cut(s) 1146
NspI RCATGY 1 cut(s) 782
OliI CACNNNNGTG 1 cut(s) 2127
PaeI GCATGC 1 cut(s) 782
PaeR7I CTCGAG 1 cut(s) 650
PagI TCATGA 1 cut(s) 195
PcsI WCGNNNNNNNCGW 1 cut(s) 129
PctI GAATGC 3 cut(s) 969, 1835, 2191
PdmI GAANNNNTTC 2 cut(s) 969, 2627
PfeI GAWTC 8 cut(s) 183, 218, 1609, 2000, 2110, 2171, 2303, 2660
PflMI CCANNNNNTGG 1 cut(s) 774
PkrI GCNGC 8 cut(s) 15, 33, 75, 381, 987, 1099, 1834, 2461
PleI GAGTC 5 cut(s) 17, 270, 351, 1309, 1798
PmeI GTTTAAAC 1 cut(s) 2311
PpsI GAGTC 5 cut(s) 17, 270, 351, 1309, 1798
Ppu21I YACGTR 3 cut(s) 133, 1171, 2154
PpuMI RGGWCCY 1 cut(s) 824
PshAI GACNNNNGTC 1 cut(s) 2083
Psp124BI GAGCTC 3 cut(s) 395, 1091, 2406
Psp5II RGGWCCY 1 cut(s) 824
Psp6I CCWGG 4 cut(s) 424, 607, 1492, 2113
PspEI GGTNACC 1 cut(s) 611
PspGI CCWGG 4 cut(s) 424, 607, 1492, 2113
PspN4I GGNNCC 5 cut(s) 254, 825, 826, 913, 1758
PspPI GGNCC 6 cut(s) 139, 252, 620, 824, 1181, 2219
PspPPI RGGWCCY 1 cut(s) 824
PspXI VCTCGAGB 1 cut(s) 650
PstNI CAGNNNCTG 1 cut(s) 1513
PsuI RGATCY 6 cut(s) 1154, 1372, 1639, 1654, 2277, 2729
PvuII CAGCTG 2 cut(s) 2024, 2296
RsaI GTAC 6 cut(s) 535, 943, 1173, 2105, 2121, 2195
RsaNI GTAC 6 cut(s) 534, 942, 1172, 2104, 2120, 2194
RseI CAYNNNNRTG 3 cut(s) 609, 1347, 2127
SacI GAGCTC 3 cut(s) 395, 1091, 2406
SaqAI TTAA 7 cut(s) 60, 677, 701, 956, 1974, 2310, 2773
SatI GCNGC 8 cut(s) 14, 32, 74, 380, 986, 1098, 1833, 2460
Sau96I GGNCC 6 cut(s) 139, 252, 620, 824, 1181, 2219
SchI GAGTC 5 cut(s) 17, 271, 351, 1310, 1798
ScrFI CCNGG 4 cut(s) 426, 609, 1494, 2115
SduI GDGCHC 4 cut(s) 395, 1008, 1091, 2406
SfaNI GCATC 5 cut(s) 96, 529, 1084, 1131, 2572
SfcI CTRYAG 1 cut(s) 854
Sfr274I CTCGAG 1 cut(s) 650
SinI GGWCC 5 cut(s) 139, 252, 620, 824, 2219
SlaI CTCGAG 1 cut(s) 650
SmiMI CAYNNNNRTG 3 cut(s) 609, 1347, 2127
SmlI CTYRAG 6 cut(s) 59, 327, 396, 650, 1463, 2281
SmoI CTYRAG 6 cut(s) 59, 327, 396, 650, 1463, 2281
SpeI ACTAGT 1 cut(s) 290
SphI GCATGC 1 cut(s) 782
SsiI CCGC 4 cut(s) 71, 379, 985, 2234
SspI AATATT 1 cut(s) 675
SstI GAGCTC 3 cut(s) 395, 1091, 2406
StyD4I CCNGG 4 cut(s) 424, 607, 1492, 2113
StyI CCWWGG 4 cut(s) 616, 901, 1747, 2324
TaiI ACGT 5 cut(s) 135, 1173, 1831, 2156, 2227
TaqII GACCGA 1 cut(s) 828
TatI WGTACW 1 cut(s) 2119
TauI GCSGC 2 cut(s) 382, 988
TfiI GAWTC 8 cut(s) 183, 218, 1609, 2000, 2110, 2171, 2303, 2660
Tru1I TTAA 7 cut(s) 60, 677, 701, 956, 1974, 2310, 2773
Tru9I TTAA 7 cut(s) 60, 677, 701, 956, 1974, 2310, 2773
TscAI CASTG 5 cut(s) 1015, 1183, 1671, 1680, 2474
TseFI GTSAC 5 cut(s) 53, 580, 611, 995, 1254
TseI GCWGC 6 cut(s) 13, 31, 73, 1097, 1832, 2459
Tsp45I GTSAC 5 cut(s) 53, 580, 611, 995, 1254
TspGWI ACGGA 1 cut(s) 16
TspRI CASTG 5 cut(s) 1015, 1183, 1671, 1680, 2474
Van91I CCANNNNNTGG 1 cut(s) 774
Vha464I CTTAAG 1 cut(s) 59
VneI GTGCAC 1 cut(s) 1004
VpaK11BI GGWCC 5 cut(s) 139, 252, 620, 824, 2219
XapI RAATTY 8 cut(s) 144, 742, 893, 1115, 1669, 1726, 1986, 2489
XbaI TCTAGA 2 cut(s) 239, 2392
XceI RCATGY 1 cut(s) 782
XcmI CCANNNNNNNNNTGG 2 cut(s) 2322, 2354
XhoI CTCGAG 1 cut(s) 650
XmiI GTMKAC 2 cut(s) 336, 1339
XmnI GAANNNNTTC 2 cut(s) 969, 2627
Zsp2I ATGCAT 1 cut(s) 1146
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.