Rh2BG064100
ERF Family

Probably involved in the RNA silencing pathway and required for the generation of small interfering RNAs (siRNAs)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0000505
Physical Location & Seq
Forward (+)
4920305 .. 4920999
695 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG064100.1

Sequence Viewer

Length: 528 bp
ATGGAGAAGAAAAACCAAGAATCCTACCATTCAAGTTCCATTTTGGGATCCATTTATGATAAGGTGGAGGAATATGAAACAGAAGACAATCTGAAAGAAATTCGGAAACTTCCTCTTTTTGAAGACGAGTACGTTCCTGAGGACCGCTTGAAGGAATGGGAGAAACGATACGAAGAGTACCGGAAGAAGATGACCTCTGCCATGGGAAATGAAGATAAAGAGAGCAAGAATCAGGCAGCCAATATCGTCATAAGAGAGTATAAAGAGATCCTATACGAGGCTGAGGAGTTTGAGGAAAGTAAAAGGGAAAGACAACAAATATATAACGACGCACTCGCTATTTATCATGTTTGTTATGACTATGCCAGGAGTGTGAGGAATGAAACTGGGGCACAAGATGTCCGGAAATGTGGCTTTGTCTGGAAAGTTGCAGGTGCAGCTCTATGCCAGCTTCATGAGGACCGGAGTTTGAAACCTGGTGAAAAGGTTTTCAGGATCTTACCCTCTGTTTTGAAGGATATTATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RdRP

Protein Analysis

175

Amino Acids

20.82

Weight (kDa)

5.23

Isoelectric Point (pI)

50.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RdRP_head PF26253 39 - 154 1.7e-14 RdRP, head domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000505)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G19910 AT2G19910 AT2G19920 AT2G19920 AT2G19920 AT2G19920 AT2G19930 AT2G19930 AT2G19930
fragaria_vesca FvH4_1g05980 FvH4_1g05990 FvH4_1g05990 FvH4_1g09718
malus_domestica MD02G1064400.v1.1 MD15G1195300.v1.1 MD15G1195400.v1.1
prunus_persica Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1
pyrus_communis pycom02g05070 pycom15g17460
rosa_chinensis RchiOBHm_Chr2g0091671 RchiOBHm_Chr2g0091681 RchiOBHm_Chr2g0091691 RchiOBHm_Chr2g0091701 RchiOBHm_Chr2g0091711 RchiOBHm_Chr2g0091721 RchiOBHm_Chr2g0091731 RchiOBHm_Chr5g0014401
rosa_laevigata RLG00000013772 RLG00000016242 RLG00000016244 RLG00000016245 RLG00000016246 RLG00000032105
rosa_multiflora Rmu_co8374041.1_g000001 Rmu_co8478121.1_g000001 Rmu_sc0000897.1_g000005 Rmu_sc0000897.1_g000009
rosa_roxburghii Rroxscaffold_1G00062180 Rroxscaffold_1G00062190 Rroxscaffold_1G00068800 Rroxscaffold_2G00149790 Rroxscaffold_2G00149800 Rroxscaffold_2G00149810
rosa_rugosa Rorug02G0019600 Rorug02G0019700 Rorug02G0019800 Rorug02G0019900 Rorug02G0019900 Rorug02G0020000 Rorug02G0020000 Rorug02G0020100 Rorug02G0020100 Rorug02G0020200 Rorug05G0017800
rosa_samantha Rh2AG064700 Rh2AG064800 Rh2AG064900 Rh2BG063800 Rh2BG063900 Rh2BG064000 Rh2BG064100 Rh2CG065500 Rh2CG065600 Rh2CG065700 Rh2DG063900 Rh2DG064000 Rh2DG064100 Rh2DG064200 Rh2DG381700 Rh5AG056700 Rh5AG056800 Rh5AG056900 Rh5AG112600 Rh5BG109300 Rh5CG063800 Rh5CG121100 Rh5DG053100 Rh5DG108300 Rh6CG100100
rosa_wichuraiana Rw2G005560 Rw2G005580 Rw2G005590 Rw5G005370 Rw5G009770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 422
Acc36I ACCTGC 1 cut(s) 422
AccIII TCCGGA 1 cut(s) 402
AciI CCGC 1 cut(s) 145
AclWI GGATC 4 cut(s) 42, 55, 262, 503
AcsI RAATTY 1 cut(s) 99
AfaI GTAC 2 cut(s) 131, 179
AfiI CCNNNNNNNGG 2 cut(s) 151, 277
AgsI TTSAA 5 cut(s) 33, 122, 151, 472, 514
AjnI CCWGG 2 cut(s) 365, 475
AluBI AGCT 2 cut(s) 440, 451
AluI AGCT 2 cut(s) 440, 451
AlwI GGATC 4 cut(s) 42, 55, 262, 503
Aor13HI TCCGGA 1 cut(s) 402
ApeKI GCWGC 2 cut(s) 236, 437
ApoI RAATTY 1 cut(s) 99
AspS9I GGNCC 2 cut(s) 142, 460
AsuHPI GGTGA 1 cut(s) 491
AvaII GGWCC 2 cut(s) 142, 460
AxyI CCTNAGG 1 cut(s) 138
BaeGI GKGCMC 1 cut(s) 394
BamHI GGATCC 1 cut(s) 47
BbsI GAAGAC 2 cut(s) 90, 129
BbvCI CCTCAGC 1 cut(s) 282
BbvI GCAGC 2 cut(s) 248, 449
BciT130I CCWGG 2 cut(s) 367, 477
BfuAI ACCTGC 1 cut(s) 422
BisI GCNGC 2 cut(s) 237, 438
BlsI GCNGC 2 cut(s) 238, 439
Bme1390I CCNGG 2 cut(s) 367, 477
Bme18I GGWCC 2 cut(s) 142, 460
BmgT120I GGNCC 2 cut(s) 142, 460
BmiI GGNNCC 1 cut(s) 49
BmrFI CCNGG 2 cut(s) 367, 477
BmrI ACTGGG 1 cut(s) 396
BmuI ACTGGG 1 cut(s) 396
BpiI GAAGAC 2 cut(s) 90, 129
Bpu10I CCTNAGC 1 cut(s) 282
BsaJI CCNNGG 1 cut(s) 201
BsaWI WCCGGW 3 cut(s) 180, 402, 462
Bsc4I CCNNNNNNNGG 2 cut(s) 151, 277
Bse1I ACTGG 1 cut(s) 391
Bse21I CCTNAGG 1 cut(s) 138
BseAI TCCGGA 1 cut(s) 402
BseBI CCWGG 2 cut(s) 367, 477
BseDI CCNNGG 1 cut(s) 201
BseLI CCNNNNNNNGG 2 cut(s) 151, 277
BseMII CTCAG 2 cut(s) 129, 273
BseNI ACTGG 1 cut(s) 391
BseRI GAGGAG 1 cut(s) 299
BseSI GKGCMC 1 cut(s) 394
BseXI GCAGC 2 cut(s) 248, 449
BsgI GTGCAG 1 cut(s) 456
BsiSI CCGG 3 cut(s) 181, 403, 463
BslI CCNNNNNNNGG 2 cut(s) 151, 277
Bsp1286I GDGCHC 1 cut(s) 394
Bsp13I TCCGGA 1 cut(s) 402
Bsp143I GATC 3 cut(s) 47, 267, 495
Bsp19I CCATGG 1 cut(s) 201
BspACI CCGC 1 cut(s) 145
BspCNI CTCAG 2 cut(s) 130, 274
BspEI TCCGGA 1 cut(s) 402
BspHI TCATGA 1 cut(s) 454
BspLI GGNNCC 1 cut(s) 49
BspMI ACCTGC 1 cut(s) 422
BspPI GGATC 4 cut(s) 42, 55, 262, 503
BsrI ACTGG 1 cut(s) 391
BssECI CCNNGG 1 cut(s) 201
BssMI GATC 3 cut(s) 47, 267, 495
BssT1I CCWWGG 1 cut(s) 201
Bst2UI CCWGG 2 cut(s) 367, 477
Bst6I CTCTTC 1 cut(s) 168
BstC8I GCNNGC 1 cut(s) 449
BstDEI CTNAG 2 cut(s) 138, 282
BstDSI CCRYGG 1 cut(s) 201
BstENI CCTNNNNNAGG 1 cut(s) 275
BstKTI GATC 3 cut(s) 50, 270, 498
BstMBI GATC 3 cut(s) 47, 267, 495
BstMWI GCNNNNNNNGC 1 cut(s) 437
BstNI CCWGG 2 cut(s) 367, 477
BstSCI CCNGG 2 cut(s) 365, 475
BstSLI GKGCMC 1 cut(s) 394
BstV1I GCAGC 2 cut(s) 248, 449
BstV2I GAAGAC 2 cut(s) 90, 129
BstX2I RGATCY 3 cut(s) 47, 267, 495
BstYI RGATCY 3 cut(s) 47, 267, 495
Bsu36I CCTNAGG 1 cut(s) 138
BtgI CCRYGG 1 cut(s) 201
BveI ACCTGC 1 cut(s) 422
Cac8I GCNNGC 1 cut(s) 449
CciI TCATGA 1 cut(s) 454
Cfr13I GGNCC 2 cut(s) 142, 460
CseI GACGC 1 cut(s) 338
CsiI ACCWGGT 1 cut(s) 475
Csp6I GTAC 2 cut(s) 130, 178
CviAII CATG 3 cut(s) 202, 347, 455
CviJI RGCY 5 cut(s) 239, 281, 414, 440, 451
CviKI_1 RGCY 5 cut(s) 239, 281, 414, 440, 451
CviQI GTAC 2 cut(s) 130, 178
DdeI CTNAG 2 cut(s) 138, 282
DpnI GATC 3 cut(s) 49, 269, 497
DpnII GATC 3 cut(s) 47, 267, 495
Eam1104I CTCTTC 1 cut(s) 168
EarI CTCTTC 1 cut(s) 168
Eco130I CCWWGG 1 cut(s) 201
Eco47I GGWCC 2 cut(s) 142, 460
Eco81I CCTNAGG 1 cut(s) 138
EcoNI CCTNNNNNAGG 1 cut(s) 275
EcoRII CCWGG 2 cut(s) 365, 475
EcoT14I CCWWGG 1 cut(s) 201
ErhI CCWWGG 1 cut(s) 201
FaeI CATG 3 cut(s) 205, 350, 458
FatI CATG 3 cut(s) 201, 346, 454
Fnu4HI GCNGC 2 cut(s) 237, 438
Fsp4HI GCNGC 2 cut(s) 237, 438
GluI GCNGC 2 cut(s) 237, 438
HapII CCGG 3 cut(s) 181, 403, 463
HgaI GACGC 1 cut(s) 338
Hin1II CATG 3 cut(s) 205, 350, 458
HinfI GANTC 2 cut(s) 20, 229
HpaII CCGG 3 cut(s) 181, 403, 463
HphI GGTGA 1 cut(s) 491
Hpy188I TCNGA 2 cut(s) 93, 105
Hpy188III TCNNGA 5 cut(s) 137, 403, 421, 455, 493
Hpy99I CGWCG 1 cut(s) 332
HpyAV CCTTC 2 cut(s) 145, 508
HpyCH4IV ACGT 1 cut(s) 132
HpyCH4V TGCA 2 cut(s) 431, 437
HpyF10VI GCNNNNNNNGC 1 cut(s) 437
HpyF3I CTNAG 2 cut(s) 138, 282
HpySE526I ACGT 1 cut(s) 132
Hsp92II CATG 3 cut(s) 205, 350, 458
Kpn2I TCCGGA 1 cut(s) 402
Kzo9I GATC 3 cut(s) 47, 267, 495
Lsp1109I GCAGC 2 cut(s) 248, 449
MabI ACCWGGT 1 cut(s) 475
MaeII ACGT 1 cut(s) 132
MalI GATC 3 cut(s) 49, 269, 497
MboI GATC 3 cut(s) 47, 267, 495
MboII GAAGA 7 cut(s) 19, 95, 134, 185, 196, 199, 224
MflI RGATCY 3 cut(s) 47, 267, 495
MhlI GDGCHC 1 cut(s) 394
MluCI AATT 1 cut(s) 99
MroI TCCGGA 1 cut(s) 402
MspI CCGG 3 cut(s) 181, 403, 463
MspR9I CCNGG 2 cut(s) 367, 477
MvaI CCWGG 2 cut(s) 367, 477
MwoI GCNNNNNNNGC 1 cut(s) 437
NcoI CCATGG 1 cut(s) 201
NdeII GATC 3 cut(s) 47, 267, 495
NlaIII CATG 3 cut(s) 205, 350, 458
NlaIV GGNNCC 1 cut(s) 49
PagI TCATGA 1 cut(s) 454
PaqCI CACCTGC 1 cut(s) 422
PfeI GAWTC 2 cut(s) 20, 229
PkrI GCNGC 2 cut(s) 238, 439
Psp6I CCWGG 2 cut(s) 365, 475
PspGI CCWGG 2 cut(s) 365, 475
PspN4I GGNNCC 1 cut(s) 49
PspPI GGNCC 2 cut(s) 142, 460
PsuI RGATCY 3 cut(s) 47, 267, 495
RsaI GTAC 2 cut(s) 131, 179
RsaNI GTAC 2 cut(s) 130, 178
SatI GCNGC 2 cut(s) 237, 438
Sau3AI GATC 3 cut(s) 47, 267, 495
Sau96I GGNCC 2 cut(s) 142, 460
ScrFI CCNGG 2 cut(s) 367, 477
SduI GDGCHC 1 cut(s) 394
SetI ASST 8 cut(s) 66, 135, 197, 436, 442, 453, 478, 489
SexAI ACCWGGT 1 cut(s) 475
SinI GGWCC 2 cut(s) 142, 460
Sse9I AATT 1 cut(s) 99
SsiI CCGC 1 cut(s) 145
StyD4I CCNGG 2 cut(s) 365, 475
StyI CCWWGG 1 cut(s) 201
TaiI ACGT 1 cut(s) 135
TasI AATT 1 cut(s) 99
TfiI GAWTC 2 cut(s) 20, 229
TseI GCWGC 2 cut(s) 236, 437
TspDTI ATGAA 4 cut(s) 90, 225, 396, 443
VpaK11BI GGWCC 2 cut(s) 142, 460
XagI CCTNNNNNAGG 1 cut(s) 275
XapI RAATTY 1 cut(s) 99
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.