Rorug02G0019900
ERF Family

Probably involved in the RNA silencing pathway and required for the generation of small interfering RNAs (siRNAs)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0000505
Physical Location & Seq
Reverse (-)
1614720 .. 1618206
3487 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0019900.1

Sequence Viewer

Length: 885 bp
ATGAAGGTAGTACTGCTTCTCACTCTACTCGCGCTCGTTCTCTGCCACCTAGTCTCGGCCGAGATCAACCCAGCTCACCTAGGTGGTACTTTTAGTCGCAGTTCTCGTGAGCCAAAGTACAAGATTGAATTCCATTCTGAAGAGTCACCTTTTCCTCCTGAAGATGATCAGGAGTCTGTGGTTATGCCCAATAAGAATGGACAGAACTTTATATGTTACATGCCTAAGGTGGAGGAAGCTAAGAGTGGGAAGCCAATTCTCCAGCAGAATACTAGCAGCATGATTGTGGAAACTGAGAAACGAGTTAAGGTGAAGACGCCAGATGAGCTGCTTGAAATACTCAAGGACCAATGCTTTATCAGACAAGAGGGTTGGTGGTCGTATGAATTTTGCTATCAGAAGAAATTACGACAAATTCATGTGGAAGATGAGAAGGTGCTTCAGGAATTTGTTTTAGGTGTATATGATGCTGAGGCCACTGCTGCATTCAACCAGAATCTCTCTGACATATCTTTGCTTAAAGATCCACGCTCAAAAGATGCATACCAAAGGTATCATCCTCATCAATATACAAACGGAACCACTTGTGATCTTACAAACCAACCTCGAGAGACTGAGGTGAGATTTGTATGCTCAGAGCCGAGAGCTATGATTAGTTCAATTACCGAGATATCCACTTGCAAGTATGCAGTTACAATTCAATGCCCCACACTTTGCAAGCATCCATTATTCCAAGCAGAGAGACCGGTATGGCAGACCATTAATTGTAATGTTCTTCCCAAGGATTACAAGGAAACCCAAGTTGCGGATGAAGTATCTGAAATTAAGCAGATAGATATGGTCACAGACAATGAATCTCAGTCCAATGGCGATTCGGATGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RdRP

Protein Analysis

294

Amino Acids

33.73

Weight (kDa)

5.11

Isoelectric Point (pI)

49.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PRKCSH PF07915 116 - 196 9.2e-18 Glucosidase II beta subunit-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000505)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G19910 AT2G19910 AT2G19920 AT2G19920 AT2G19920 AT2G19920 AT2G19930 AT2G19930 AT2G19930
fragaria_vesca FvH4_1g05980 FvH4_1g05990 FvH4_1g05990 FvH4_1g09718
malus_domestica MD02G1064400.v1.1 MD15G1195300.v1.1 MD15G1195400.v1.1
prunus_persica Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1
pyrus_communis pycom02g05070 pycom15g17460
rosa_chinensis RchiOBHm_Chr2g0091671 RchiOBHm_Chr2g0091681 RchiOBHm_Chr2g0091691 RchiOBHm_Chr2g0091701 RchiOBHm_Chr2g0091711 RchiOBHm_Chr2g0091721 RchiOBHm_Chr2g0091731 RchiOBHm_Chr5g0014401
rosa_laevigata RLG00000013772 RLG00000016242 RLG00000016244 RLG00000016245 RLG00000016246 RLG00000032105
rosa_multiflora Rmu_co8374041.1_g000001 Rmu_co8478121.1_g000001 Rmu_sc0000897.1_g000005 Rmu_sc0000897.1_g000009
rosa_roxburghii Rroxscaffold_1G00062180 Rroxscaffold_1G00062190 Rroxscaffold_1G00068800 Rroxscaffold_2G00149790 Rroxscaffold_2G00149800 Rroxscaffold_2G00149810
rosa_rugosa Rorug02G0019600 Rorug02G0019700 Rorug02G0019800 Rorug02G0019900 Rorug02G0019900 Rorug02G0020000 Rorug02G0020000 Rorug02G0020100 Rorug02G0020100 Rorug02G0020200 Rorug05G0017800
rosa_samantha Rh2AG064700 Rh2AG064800 Rh2AG064900 Rh2BG063800 Rh2BG063900 Rh2BG064000 Rh2BG064100 Rh2CG065500 Rh2CG065600 Rh2CG065700 Rh2DG063900 Rh2DG064000 Rh2DG064100 Rh2DG064200 Rh2DG381700 Rh5AG056700 Rh5AG056800 Rh5AG056900 Rh5AG112600 Rh5BG109300 Rh5CG063800 Rh5CG121100 Rh5DG053100 Rh5DG108300 Rh6CG100100
rosa_wichuraiana Rw2G005560 Rw2G005580 Rw2G005590 Rw5G005370 Rw5G009770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 32
AciI CCGC 1 cut(s) 806
AclWI GGATC 1 cut(s) 518
AcoI YGGCCR 1 cut(s) 57
AcsI RAATTY 4 cut(s) 128, 386, 414, 446
AcuI CTGAAG 3 cut(s) 159, 180, 425
AcyI GRCGYC 1 cut(s) 317
AfaI GTAC 3 cut(s) 12, 88, 119
AfiI CCNNNNNNNGG 2 cut(s) 55, 805
AgeI ACCGGT 1 cut(s) 745
AgsI TTSAA 5 cut(s) 128, 335, 490, 660, 701
AleI CACNNNNGTG 1 cut(s) 81
AluBI AGCT 4 cut(s) 74, 239, 328, 647
AluI AGCT 4 cut(s) 74, 239, 328, 647
Alw26I GTCTC 3 cut(s) 58, 605, 736
AlwI GGATC 1 cut(s) 518
Ama87I CYCGRG 1 cut(s) 606
AoxI GGCC 2 cut(s) 57, 474
ApeKI GCWGC 3 cut(s) 276, 328, 482
ApoI RAATTY 4 cut(s) 128, 386, 414, 446
AseI ATTAAT 1 cut(s) 762
AsiGI ACCGGT 1 cut(s) 745
AspA2I CCTAGG 1 cut(s) 79
AspLEI GCGC 1 cut(s) 34
AspS9I GGNCC 1 cut(s) 346
AsuHPI GGTGA 4 cut(s) 68, 138, 322, 631
AvaI CYCGRG 1 cut(s) 606
AvaII GGWCC 1 cut(s) 346
AvrII CCTAGG 1 cut(s) 79
AxyI CCTNAGG 1 cut(s) 225
BarI GAAGNNNNNNTAC 1 cut(s) 32
BauI CACGAG 1 cut(s) 105
BbsI GAAGAC 1 cut(s) 320
BbvCI CCTCAGC 1 cut(s) 471
BbvI GCAGC 3 cut(s) 288, 315, 469
BclI TGATCA 1 cut(s) 166
BcoDI GTCTC 3 cut(s) 58, 605, 736
BfaI CTAG 3 cut(s) 50, 80, 273
BisI GCNGC 3 cut(s) 277, 329, 483
BlnI CCTAGG 1 cut(s) 79
BlsI GCNGC 3 cut(s) 278, 330, 484
BmcAI AGTACT 1 cut(s) 12
Bme18I GGWCC 1 cut(s) 346
BmeT110I CYCGRG 1 cut(s) 606
BmgT120I GGNCC 1 cut(s) 346
BmiI GGNNCC 1 cut(s) 580
BmsI GCATC 3 cut(s) 457, 529, 730
BpiI GAAGAC 1 cut(s) 320
BpmI CTGGAG 1 cut(s) 245
Bpu10I CCTNAGC 1 cut(s) 471
BpuEI CTTGAG 1 cut(s) 326
BsaHI GRCGYC 1 cut(s) 317
BsaI GGTCTC 1 cut(s) 736
BsaJI CCNNGG 2 cut(s) 79, 780
BsaWI WCCGGW 1 cut(s) 745
Bsc4I CCNNNNNNNGG 2 cut(s) 55, 805
Bse118I RCCGGY 1 cut(s) 745
Bse21I CCTNAGG 1 cut(s) 225
BseDI CCNNGG 2 cut(s) 79, 780
BseGI GGATG 4 cut(s) 556, 721, 814, 883
BseLI CCNNNNNNNGG 2 cut(s) 55, 805
BseMII CTCAG 5 cut(s) 285, 462, 606, 648, 872
BseX3I CGGCCG 1 cut(s) 57
BseXI GCAGC 3 cut(s) 288, 315, 469
BseYI CCCAGC 1 cut(s) 70
Bsh1236I CGCG 1 cut(s) 32
Bsh1285I CGRYCG 1 cut(s) 60
BshFI GGCC 2 cut(s) 59, 476
BshTI ACCGGT 1 cut(s) 745
BsiEI CGRYCG 1 cut(s) 60
BsiHKCI CYCGRG 1 cut(s) 606
BsiSI CCGG 1 cut(s) 746
BslI CCNNNNNNNGG 2 cut(s) 55, 805
BsmAI GTCTC 3 cut(s) 58, 605, 736
BsmI GAATGC 1 cut(s) 485
BsnI GGCC 2 cut(s) 59, 476
Bso31I GGTCTC 1 cut(s) 736
BsoBI CYCGRG 1 cut(s) 606
Bsp143I GATC 4 cut(s) 63, 166, 523, 589
BspACI CCGC 1 cut(s) 806
BspANI GGCC 2 cut(s) 59, 476
BspCNI CTCAG 5 cut(s) 286, 463, 607, 647, 871
BspFNI CGCG 1 cut(s) 32
BspLI GGNNCC 1 cut(s) 580
BspPI GGATC 1 cut(s) 518
BspTNI GGTCTC 1 cut(s) 736
BsrFI RCCGGY 1 cut(s) 745
BssAI RCCGGY 1 cut(s) 745
BssECI CCNNGG 2 cut(s) 79, 780
BssMI GATC 4 cut(s) 63, 166, 523, 589
BssNI GRCGYC 1 cut(s) 317
BssSI CACGAG 1 cut(s) 105
BssT1I CCWWGG 2 cut(s) 79, 780
Bst2BI CACGAG 1 cut(s) 105
Bst6I CTCTTC 1 cut(s) 135
BstACI GRCGYC 1 cut(s) 317
BstC8I GCNNGC 1 cut(s) 719
BstDEI CTNAG 7 cut(s) 225, 240, 294, 471, 615, 634, 858
BstF5I GGATG 4 cut(s) 556, 721, 814, 883
BstFNI CGCG 1 cut(s) 32
BstHHI GCGC 1 cut(s) 34
BstKTI GATC 4 cut(s) 66, 169, 526, 592
BstMAI GTCTC 3 cut(s) 58, 605, 736
BstMBI GATC 4 cut(s) 63, 166, 523, 589
BstMCI CGRYCG 1 cut(s) 60
BstMWI GCNNNNNNNGC 2 cut(s) 325, 482
BstNSI RCATGY 1 cut(s) 223
BstUI CGCG 1 cut(s) 32
BstV1I GCAGC 3 cut(s) 288, 315, 469
BstV2I GAAGAC 1 cut(s) 320
BstX2I RGATCY 1 cut(s) 523
BstYI RGATCY 1 cut(s) 523
BstZI CGGCCG 1 cut(s) 57
Bsu36I CCTNAGG 1 cut(s) 225
BsuRI GGCC 2 cut(s) 59, 476
BtsCI GGATG 4 cut(s) 556, 721, 814, 883
BtsI GCAGTG 1 cut(s) 477
BtsIMutI CAGTG 1 cut(s) 477
Cac8I GCNNGC 1 cut(s) 719
CfoI GCGC 1 cut(s) 34
Cfr10I RCCGGY 1 cut(s) 745
Cfr13I GGNCC 1 cut(s) 346
CseI GACGC 1 cut(s) 325
Csp6I GTAC 3 cut(s) 11, 87, 118
CspAI ACCGGT 1 cut(s) 745
CviAII CATG 3 cut(s) 220, 280, 419
CviJI RGCY 9 cut(s) 59, 74, 112, 239, 253, 328, 476, 640, 647
CviKI_1 RGCY 9 cut(s) 59, 74, 112, 239, 253, 328, 476, 640, 647
CviQI GTAC 3 cut(s) 11, 87, 118
DdeI CTNAG 7 cut(s) 225, 240, 294, 471, 615, 634, 858
DpnI GATC 4 cut(s) 65, 168, 525, 591
DpnII GATC 4 cut(s) 63, 166, 523, 589
EaeI YGGCCR 1 cut(s) 57
EagI CGGCCG 1 cut(s) 57
Eam1104I CTCTTC 1 cut(s) 135
EarI CTCTTC 1 cut(s) 135
EclXI CGGCCG 1 cut(s) 57
Eco130I CCWWGG 2 cut(s) 79, 780
Eco31I GGTCTC 1 cut(s) 736
Eco32I GATATC 1 cut(s) 672
Eco47I GGWCC 1 cut(s) 346
Eco52I CGGCCG 1 cut(s) 57
Eco57I CTGAAG 3 cut(s) 159, 180, 425
Eco81I CCTNAGG 1 cut(s) 225
Eco88I CYCGRG 1 cut(s) 606
EcoRI GAATTC 1 cut(s) 128
EcoRV GATATC 1 cut(s) 672
EcoT14I CCWWGG 2 cut(s) 79, 780
EcoT22I ATGCAT 1 cut(s) 544
ErhI CCWWGG 2 cut(s) 79, 780
FaeI CATG 3 cut(s) 223, 283, 422
FatI CATG 3 cut(s) 219, 279, 418
FbaI TGATCA 1 cut(s) 166
Fnu4HI GCNGC 3 cut(s) 277, 329, 483
FokI GGATG 3 cut(s) 543, 708, 821
Fsp4HI GCNGC 3 cut(s) 277, 329, 483
FspBI CTAG 3 cut(s) 50, 80, 273
GlaI GCGC 1 cut(s) 33
GluI GCNGC 3 cut(s) 277, 329, 483
GsaI CCCAGC 1 cut(s) 74
GsuI CTGGAG 1 cut(s) 245
HaeIII GGCC 2 cut(s) 59, 476
HapII CCGG 1 cut(s) 746
HgaI GACGC 1 cut(s) 325
HhaI GCGC 1 cut(s) 34
Hin1I GRCGYC 1 cut(s) 317
Hin1II CATG 3 cut(s) 223, 283, 422
Hin6I GCGC 1 cut(s) 32
HinP1I GCGC 1 cut(s) 32
HinfI GANTC 5 cut(s) 143, 173, 496, 854, 872
HpaII CCGG 1 cut(s) 746
HphI GGTGA 4 cut(s) 68, 138, 322, 631
Hpy188I TCNGA 7 cut(s) 139, 362, 399, 505, 637, 820, 877
Hpy188III TCNNGA 5 cut(s) 107, 158, 170, 443, 608
HpyAV CCTTC 1 cut(s) 427
HpyCH4V TGCA 5 cut(s) 485, 542, 681, 689, 717
HpyF10VI GCNNNNNNNGC 2 cut(s) 325, 482
HpyF3I CTNAG 7 cut(s) 225, 240, 294, 471, 615, 634, 858
Hsp92I GRCGYC 1 cut(s) 317
Hsp92II CATG 3 cut(s) 223, 283, 422
HspAI GCGC 1 cut(s) 32
Ksp22I TGATCA 1 cut(s) 166
Kzo9I GATC 4 cut(s) 63, 166, 523, 589
LpnPI CCDG 8 cut(s) 84, 155, 171, 275, 333, 428, 506, 759
Lsp1109I GCAGC 3 cut(s) 288, 315, 469
LweI GCATC 3 cut(s) 457, 529, 730
MaeI CTAG 3 cut(s) 50, 80, 273
MaeIII GTNAC 4 cut(s) 144, 215, 691, 841
MalI GATC 4 cut(s) 65, 168, 525, 591
MboI GATC 4 cut(s) 63, 166, 523, 589
MboII GAAGA 6 cut(s) 152, 173, 325, 412, 437, 767
MflI RGATCY 1 cut(s) 523
MlyI GAGTC 2 cut(s) 152, 182
MnlI CCTC 7 cut(s) 165, 226, 361, 466, 570, 610, 615
Mph1103I ATGCAT 1 cut(s) 544
MseI TTAA 4 cut(s) 306, 519, 762, 825
MslI CAYNNNNRTG 2 cut(s) 81, 284
MspI CCGG 1 cut(s) 746
Mva1269I GAATGC 1 cut(s) 485
MvnI CGCG 1 cut(s) 32
MwoI GCNNNNNNNGC 2 cut(s) 325, 482
NdeII GATC 4 cut(s) 63, 166, 523, 589
NlaIII CATG 3 cut(s) 223, 283, 422
NlaIV GGNNCC 1 cut(s) 580
NmeAIII GCCGAG 3 cut(s) 35, 85, 666
NmuCI GTSAC 2 cut(s) 144, 841
NsiI ATGCAT 1 cut(s) 544
NspI RCATGY 1 cut(s) 223
OliI CACNNNNGTG 1 cut(s) 81
PaeR7I CTCGAG 1 cut(s) 606
PcsI WCGNNNNNNNCGW 1 cut(s) 103
PctI GAATGC 1 cut(s) 485
PfeI GAWTC 3 cut(s) 496, 854, 872
PinAI ACCGGT 1 cut(s) 745
PkrI GCNGC 3 cut(s) 278, 330, 484
PleI GAGTC 2 cut(s) 151, 181
PpsI GAGTC 2 cut(s) 151, 181
PshBI ATTAAT 1 cut(s) 762
PspFI CCCAGC 1 cut(s) 70
PspN4I GGNNCC 1 cut(s) 580
PspPI GGNCC 1 cut(s) 346
PsuI RGATCY 1 cut(s) 523
RsaI GTAC 3 cut(s) 12, 88, 119
RsaNI GTAC 3 cut(s) 11, 87, 118
RseI CAYNNNNRTG 2 cut(s) 81, 284
SaqAI TTAA 4 cut(s) 306, 519, 762, 825
SatI GCNGC 3 cut(s) 277, 329, 483
Sau3AI GATC 4 cut(s) 63, 166, 523, 589
Sau96I GGNCC 1 cut(s) 346
ScaI AGTACT 1 cut(s) 12
SchI GAGTC 2 cut(s) 152, 182
SfaNI GCATC 3 cut(s) 457, 529, 730
Sfr274I CTCGAG 1 cut(s) 606
SinI GGWCC 1 cut(s) 346
SlaI CTCGAG 1 cut(s) 606
SmiMI CAYNNNNRTG 2 cut(s) 81, 284
SmlI CTYRAG 2 cut(s) 341, 606
SmoI CTYRAG 2 cut(s) 341, 606
SsiI CCGC 1 cut(s) 806
SspMI CTAG 3 cut(s) 50, 80, 273
StyI CCWWGG 2 cut(s) 79, 780
TaqI TCGA 1 cut(s) 607
TatI WGTACW 2 cut(s) 10, 117
TfiI GAWTC 3 cut(s) 496, 854, 872
Tru1I TTAA 4 cut(s) 306, 519, 762, 825
Tru9I TTAA 4 cut(s) 306, 519, 762, 825
TscAI CASTG 1 cut(s) 484
TseFI GTSAC 2 cut(s) 144, 841
TseI GCWGC 3 cut(s) 276, 328, 482
Tsp45I GTSAC 2 cut(s) 144, 841
TspDTI ATGAA 5 cut(s) 17, 399, 407, 825, 867
TspGWI ACGGA 1 cut(s) 591
TspRI CASTG 1 cut(s) 484
VpaK11BI GGWCC 1 cut(s) 346
VspI ATTAAT 1 cut(s) 762
XapI RAATTY 4 cut(s) 128, 386, 414, 446
XceI RCATGY 1 cut(s) 223
XhoI CTCGAG 1 cut(s) 606
XmaJI CCTAGG 1 cut(s) 79
XspI CTAG 3 cut(s) 50, 80, 273
ZrmI AGTACT 1 cut(s) 12
Zsp2I ATGCAT 1 cut(s) 544
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.