Rh2DG063900
ERF Family

Probably involved in the RNA silencing pathway and required for the generation of small interfering RNAs (siRNAs)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0000505
Physical Location & Seq
Forward (+)
4773013 .. 4779619
6607 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG063900.1

Sequence Viewer

Length: 1824 bp
ATGGAAGAAGTGAGATTACCTCCGCCGGTGGAGGAGTTGCTCCGTAGAATCTGTACTGAGAAAAAGCTACCATGGCCGGACGCCGGGGTCCGGCGAGAGCTGGCCTCTATCAGCCAGGAACGTGCACATGAGGTCCTGGTTAAAGTCTTCAACAAACAAGATATCATAACTCTCGGCGGTTTCATCAAGTACTTGATCCGTAACCTCCCTGCTTCTCCTCCTCCAACAGCTGTCTGCGCTTATTATGATCAAAGCCCCTCTGCTTGTCCAGTTACGCCACCTTGTTCTCAACTTCAGTACAGCTCCGCAGTTGCAGGTCAGGCACTCAGTGATCGGACAGAGCAGCCTGTGATTAATGGACGGTATGGACCATCACCATATTCTTCTGGTTGTAAAGCCATCAGCTCTTTCTCGTATCCAACAATACCGCTGGCTTGTAACATTGCTCCAGTGCAACTTGATCATAGGTTTCAAGGTGAGGTTGTAACCAAGTCGCCAGATCTAGAGAAATATGGAGGCCATGGACAAGCACACTTGGCAGCTCTTGGTACTCTTGAATTCAGAAAACAATTTCTGATCTTGAGCTACATTAGAGAGGAAAAGTTAAAGACTGTTATAGTCGCAGAGGATATTAAGCATTTGAAAGATTTGCCAATGGCAGAGTTTGAGTCCAAAGTTTGTCTACTCTTCGGACATAAATATATCAGCAATGGAGACCGACAGTTGAATTTCGATTGGGATGGCAGACGCACACATTTCTATGAGTGCCATGTTACCAGAGATGGGAGTTACACATTCAAGGGCCCCTATCTACAAAATGAAAGAAGAACTCTCCTACAGAAACTGTGTAAAGATGACAATGTTTTGATTGTGAAGTTTGATGAAGAAGGGACTCAGACAGGTTCTAGGACTTCTTATGATAATGACTATTCTACGTATATGAAGGTTGAGAGACAAGGGATTTTTGTTGGCTTGCGGCGCTATCGTTTCTTTGGTTTTAAAGATGGTGGTAAAAGAGATAAAATCAGAAGTCCCACGTCATCACCCGTAAAATGTTATTTTGTTCGTGTGAGCTCTGATGCAGCAATAGACAGGGATGTAGGTTATTGGTTATCTAACAGAAGAATTCATGAAGGAAGACGTCTAATTATGCATGTTGATACTCTGCCAAGTGTGGACAAGGTCATGGCTAGGTTGCCACTCATTTTTTCTAAGACAACTACCTTGGAAGTAGATTGGTCACTTGTGAAAGTTGAAGAAATTGTTGATGTCAACTGTTGCGATGAATCTGGTAACTGTGTTTATAATGAAGATGGAAAACCTCTTCTACATACAGATGGAACTGGATTCATATCTGAGGATTTGGCTTTACTTTGTCCAAAGATAGAAGCCAAAAGAAAGTTTATGAGTGGGGAACACATGGAGGGACTTCCGGATCCTGATGAACTTGAGATTGAAATGAAAAAATCAGATCTCCGAACTCAAGAACCGCCATTGCTCATACAGTGCCGCCTATTTCACGAAGGCAATGCTTATAAAGGAACTTTGCTTGTCAATAAGCGGCTTCCCCCGAAAACACTGCAGATTCGACCGTCATTGCGTAAAGTTGAGAAGGACCCTAAGATTTCAGATAATGAAACCGTCAATTCTTTGGAAATAGTTTGCACAAGTGCTCCTCCTAGGAAAGCTTATTTGTCAAGGATTATAATTGCACTTCTAGTGCATGGAGGTGTACCGAAAGACTTCTTTATGAAGATACTTGACAATGCTTTAGAAGATGCTCATCGTGTTATCGGGAAGACGTGCAGCGCTCAAAGTTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RdRP

Protein Analysis

607

Amino Acids

68.65

Weight (kDa)

8.28

Isoelectric Point (pI)

52.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
4HB_RdRP3_N PF26249 4 - 67 9.6e-09 RdRP3/4/5, N-terminal four helical bundle domain
RdRP_helical PF26252 178 - 242 1.2e-06 RNA dependent RNA polymerase, helical domain
RdRP PF05183 264 - 598 6.9e-43 RNA dependent RNA polymerase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000505)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G19910 AT2G19910 AT2G19920 AT2G19920 AT2G19920 AT2G19920 AT2G19930 AT2G19930 AT2G19930
fragaria_vesca FvH4_1g05980 FvH4_1g05990 FvH4_1g05990 FvH4_1g09718
malus_domestica MD02G1064400.v1.1 MD15G1195300.v1.1 MD15G1195400.v1.1
prunus_persica Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1
pyrus_communis pycom02g05070 pycom15g17460
rosa_chinensis RchiOBHm_Chr2g0091671 RchiOBHm_Chr2g0091681 RchiOBHm_Chr2g0091691 RchiOBHm_Chr2g0091701 RchiOBHm_Chr2g0091711 RchiOBHm_Chr2g0091721 RchiOBHm_Chr2g0091731 RchiOBHm_Chr5g0014401
rosa_laevigata RLG00000013772 RLG00000016242 RLG00000016244 RLG00000016245 RLG00000016246 RLG00000032105
rosa_multiflora Rmu_co8374041.1_g000001 Rmu_co8478121.1_g000001 Rmu_sc0000897.1_g000005 Rmu_sc0000897.1_g000009
rosa_roxburghii Rroxscaffold_1G00062180 Rroxscaffold_1G00062190 Rroxscaffold_1G00068800 Rroxscaffold_2G00149790 Rroxscaffold_2G00149800 Rroxscaffold_2G00149810
rosa_rugosa Rorug02G0019600 Rorug02G0019700 Rorug02G0019800 Rorug02G0019900 Rorug02G0019900 Rorug02G0020000 Rorug02G0020000 Rorug02G0020100 Rorug02G0020100 Rorug02G0020200 Rorug05G0017800
rosa_samantha Rh2AG064700 Rh2AG064800 Rh2AG064900 Rh2BG063800 Rh2BG063900 Rh2BG064000 Rh2BG064100 Rh2CG065500 Rh2CG065600 Rh2CG065700 Rh2DG063900 Rh2DG064000 Rh2DG064100 Rh2DG064200 Rh2DG381700 Rh5AG056700 Rh5AG056800 Rh5AG056900 Rh5AG112600 Rh5BG109300 Rh5CG063800 Rh5CG121100 Rh5DG053100 Rh5DG108300 Rh6CG100100
rosa_wichuraiana Rw2G005560 Rw2G005580 Rw2G005590 Rw5G005370 Rw5G009770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 1305, 1536, 1706
AasI GACNNNNNNGTC 1 cut(s) 86
AatII GACGTC 1 cut(s) 1144
Acc36I ACCTGC 1 cut(s) 305
AccI GTMKAC 1 cut(s) 682
AccIII TCCGGA 1 cut(s) 1432
AciI CCGC 8 cut(s) 23, 177, 306, 428, 976, 1490, 1510, 1561
AclWI GGATC 3 cut(s) 190, 1430, 1443
AcoI YGGCCR 1 cut(s) 74
AcsI RAATTY 3 cut(s) 557, 727, 1125
AcuI CTGAAG 1 cut(s) 278
AcyI GRCGYC 2 cut(s) 81, 1141
AdeI CACNNNGTG 1 cut(s) 329
AfaI GTAC 5 cut(s) 55, 191, 299, 550, 1734
AfeI AGCGCT 1 cut(s) 1810
AfiI CCNNNNNNNGG 3 cut(s) 83, 90, 783
AgsI TTSAA 8 cut(s) 151, 473, 557, 643, 727, 799, 1256, 1457
AjiI CACGTC 2 cut(s) 1038, 1803
AjnI CCWGG 2 cut(s) 114, 135
AluBI AGCT 9 cut(s) 67, 100, 230, 303, 405, 542, 585, 1074, 1688
AluI AGCT 9 cut(s) 67, 100, 230, 303, 405, 542, 585, 1074, 1688
Alw21I GWGCWC 3 cut(s) 127, 1076, 1675
Alw26I GTCTC 2 cut(s) 708, 946
Alw44I GTGCAC 1 cut(s) 123
AlwI GGATC 3 cut(s) 190, 1430, 1443
AlwNI CAGNNNCTG 1 cut(s) 844
Aor13HI TCCGGA 1 cut(s) 1432
Aor51HI AGCGCT 1 cut(s) 1810
AoxI GGCC 4 cut(s) 74, 102, 517, 802
ApaI GGGCCC 1 cut(s) 806
ApaLI GTGCAC 1 cut(s) 123
ApeKI GCWGC 4 cut(s) 343, 539, 1082, 1806
ApoI RAATTY 3 cut(s) 557, 727, 1125
AseI ATTAAT 1 cut(s) 354
Asp700I GAANNNNTTC 1 cut(s) 1742
AspA2I CCTAGG 1 cut(s) 1679
AspLEI GCGC 3 cut(s) 239, 981, 1811
AspS9I GGNCC 6 cut(s) 88, 133, 368, 802, 803, 1615
AsuC2I CCSGG 1 cut(s) 85
AsuHPI GGTGA 3 cut(s) 366, 488, 1035
AvaII GGWCC 4 cut(s) 88, 133, 368, 1615
AvrII CCTAGG 1 cut(s) 1679
BaeGI GKGCMC 2 cut(s) 127, 806
BamHI GGATCC 1 cut(s) 1435
BanII GRGCYC 2 cut(s) 806, 1076
BarI GAAGNNNNNNTAC 1 cut(s) 32
BbsI GAAGAC 3 cut(s) 139, 1144, 1805
Bbv12I GWGCWC 3 cut(s) 127, 1076, 1675
BbvI GCAGC 4 cut(s) 355, 551, 1094, 1818
BccI CCATC 7 cut(s) 379, 407, 734, 776, 998, 1307, 1331
BcgI CGANNNNNNTGC 4 cut(s) 1511, 1545, 1561, 1595
BciT130I CCWGG 2 cut(s) 116, 137
BciVI GTATCC 1 cut(s) 426
BclI TGATCA 2 cut(s) 247, 460
BcnI CCSGG 1 cut(s) 85
BcoDI GTCTC 2 cut(s) 708, 946
BfaI CTAG 5 cut(s) 503, 906, 1191, 1680, 1718
BfmI CTRYAG 2 cut(s) 836, 1580
BfoI RGCGCY 2 cut(s) 982, 1812
BfuAI ACCTGC 1 cut(s) 305
BfuI GTATCC 1 cut(s) 426
BglII AGATCT 2 cut(s) 499, 1471
BisI GCNGC 7 cut(s) 344, 540, 977, 1083, 1510, 1562, 1807
BlnI CCTAGG 1 cut(s) 1679
BlsI GCNGC 7 cut(s) 345, 541, 978, 1084, 1511, 1563, 1808
BmcAI AGTACT 1 cut(s) 191
Bme1390I CCNGG 3 cut(s) 85, 116, 137
Bme18I GGWCC 4 cut(s) 88, 133, 368, 1615
BmgBI CACGTC 2 cut(s) 1038, 1803
BmgT120I GGNCC 6 cut(s) 88, 133, 368, 802, 803, 1615
BmiI GGNNCC 5 cut(s) 89, 804, 805, 1437, 1617
BmrFI CCNGG 3 cut(s) 85, 116, 137
BmsI GCATC 2 cut(s) 1069, 1768
BpiI GAAGAC 3 cut(s) 139, 1144, 1805
BplI GAGNNNNNCTC 3 cut(s) 36, 89, 121
BpmI CTGGAG 1 cut(s) 432
BpuEI CTTGAG 3 cut(s) 601, 1467, 1469
BpuMI CCSGG 1 cut(s) 85
BsaAI YACGTR 1 cut(s) 936
BsaBI GATNNNNATC 2 cut(s) 1351, 1782
BsaHI GRCGYC 2 cut(s) 81, 1141
BsaI GGTCTC 1 cut(s) 708
BsaJI CCNNGG 5 cut(s) 71, 84, 520, 1224, 1679
BsaWI WCCGGW 1 cut(s) 1432
BsaXI ACNNNNNCTCC 2 cut(s) 1657, 1687
Bsc4I CCNNNNNNNGG 3 cut(s) 83, 90, 783
Bse118I RCCGGY 1 cut(s) 25
Bse1I ACTGG 3 cut(s) 269, 449, 1348
Bse3DI GCAATG 5 cut(s) 441, 715, 1493, 1534, 1595
Bse8I GATNNNNATC 2 cut(s) 1351, 1782
BseAI TCCGGA 1 cut(s) 1432
BseBI CCWGG 2 cut(s) 116, 137
BseDI CCNNGG 5 cut(s) 71, 84, 520, 1224, 1679
BseGI GGATG 2 cut(s) 745, 1102
BseJI GATNNNNATC 2 cut(s) 1351, 1782
BseLI CCNNNNNNNGG 3 cut(s) 83, 90, 783
BseMI GCAATG 5 cut(s) 441, 715, 1493, 1534, 1595
BseMII CTCAG 4 cut(s) 48, 340, 908, 1347
BseNI ACTGG 3 cut(s) 269, 449, 1348
BseRI GAGGAG 4 cut(s) 47, 207, 210, 1665
BseSI GKGCMC 2 cut(s) 127, 806
BseXI GCAGC 4 cut(s) 355, 551, 1094, 1818
Bsh1285I CGRYCG 1 cut(s) 1592
BshFI GGCC 4 cut(s) 76, 104, 519, 804
BsiEI CGRYCG 1 cut(s) 1592
BsiHKAI GWGCWC 3 cut(s) 127, 1076, 1675
BsiSI CCGG 5 cut(s) 26, 77, 84, 91, 1433
BslFI GGGAC 3 cut(s) 904, 1017, 1440
BslI CCNNNNNNNGG 3 cut(s) 83, 90, 783
BsmAI GTCTC 2 cut(s) 708, 946
BsmFI GGGAC 3 cut(s) 904, 1017, 1440
BsnI GGCC 4 cut(s) 76, 104, 519, 804
Bso31I GGTCTC 1 cut(s) 708
Bsp120I GGGCCC 1 cut(s) 802
Bsp1286I GDGCHC 4 cut(s) 127, 806, 1076, 1675
Bsp13I TCCGGA 1 cut(s) 1432
Bsp143I GATC 8 cut(s) 195, 247, 331, 460, 499, 576, 1435, 1471
Bsp19I CCATGG 2 cut(s) 71, 520
BspACI CCGC 8 cut(s) 23, 177, 306, 428, 976, 1490, 1510, 1561
BspANI GGCC 4 cut(s) 76, 104, 519, 804
BspCNI CTCAG 4 cut(s) 49, 339, 907, 1348
BspEI TCCGGA 1 cut(s) 1432
BspHI TCATGA 1 cut(s) 1129
BspLI GGNNCC 5 cut(s) 89, 804, 805, 1437, 1617
BspMAI CTGCAG 1 cut(s) 1584
BspMI ACCTGC 1 cut(s) 305
BspPI GGATC 3 cut(s) 190, 1430, 1443
BspTNI GGTCTC 1 cut(s) 708
BsrDI GCAATG 5 cut(s) 441, 715, 1493, 1534, 1595
BsrFI RCCGGY 1 cut(s) 25
BsrI ACTGG 3 cut(s) 269, 449, 1348
BssAI RCCGGY 1 cut(s) 25
BssECI CCNNGG 5 cut(s) 71, 84, 520, 1224, 1679
BssMI GATC 8 cut(s) 195, 247, 331, 460, 499, 576, 1435, 1471
BssNI GRCGYC 2 cut(s) 81, 1141
BssT1I CCWWGG 4 cut(s) 71, 520, 1224, 1679
Bst2UI CCWGG 2 cut(s) 116, 137
Bst4CI ACNGT 9 cut(s) 363, 613, 723, 846, 1277, 1298, 1506, 1593, 1642
Bst6I CTCTTC 2 cut(s) 692, 1329
BstACI GRCGYC 2 cut(s) 81, 1141
BstBAI YACGTR 1 cut(s) 936
BstC8I GCNNGC 3 cut(s) 102, 432, 974
BstDEI CTNAG 6 cut(s) 57, 326, 894, 1212, 1356, 1620
BstDSI CCRYGG 2 cut(s) 71, 520
BstF5I GGATG 2 cut(s) 745, 1102
BstH2I RGCGCY 2 cut(s) 982, 1812
BstHHI GCGC 3 cut(s) 239, 981, 1811
BstKTI GATC 8 cut(s) 198, 250, 334, 463, 502, 579, 1438, 1474
BstMAI GTCTC 2 cut(s) 708, 946
BstMBI GATC 8 cut(s) 195, 247, 331, 460, 499, 576, 1435, 1471
BstMCI CGRYCG 1 cut(s) 1592
BstMWI GCNNNNNNNGC 5 cut(s) 73, 236, 320, 536, 978
BstNI CCWGG 2 cut(s) 116, 137
BstNSI RCATGY 1 cut(s) 1157
BstSCI CCNGG 3 cut(s) 83, 114, 135
BstSFI CTRYAG 2 cut(s) 836, 1580
BstSLI GKGCMC 2 cut(s) 127, 806
BstSNI TACGTA 1 cut(s) 936
BstV1I GCAGC 4 cut(s) 355, 551, 1094, 1818
BstV2I GAAGAC 3 cut(s) 139, 1144, 1805
BstX2I RGATCY 3 cut(s) 499, 1435, 1471
BstYI RGATCY 3 cut(s) 499, 1435, 1471
BsuI GTATCC 1 cut(s) 426
BsuRI GGCC 4 cut(s) 76, 104, 519, 804
BtgI CCRYGG 2 cut(s) 71, 520
BtgZI GCGATG 1 cut(s) 1296
BtrI CACGTC 2 cut(s) 1038, 1803
BtsCI GGATG 2 cut(s) 745, 1102
BtsI GCAGTG 1 cut(s) 1577
BtsIMutI CAGTG 4 cut(s) 334, 456, 1511, 1577
BveI ACCTGC 1 cut(s) 305
Cac8I GCNNGC 3 cut(s) 102, 432, 974
CaiI CAGNNNCTG 1 cut(s) 844
CciI TCATGA 1 cut(s) 1129
CfoI GCGC 3 cut(s) 239, 981, 1811
Cfr10I RCCGGY 1 cut(s) 25
Cfr13I GGNCC 6 cut(s) 88, 133, 368, 802, 803, 1615
CseI GACGC 2 cut(s) 89, 756
Csp6I GTAC 5 cut(s) 54, 190, 298, 549, 1733
CviAII CATG 9 cut(s) 72, 128, 521, 770, 1130, 1154, 1186, 1420, 1724
CviQI GTAC 5 cut(s) 54, 190, 298, 549, 1733
DdeI CTNAG 6 cut(s) 57, 326, 894, 1212, 1356, 1620
DpnI GATC 8 cut(s) 197, 249, 333, 462, 501, 578, 1437, 1473
DpnII GATC 8 cut(s) 195, 247, 331, 460, 499, 576, 1435, 1471
DraI TTTAAA 1 cut(s) 1000
DraIII CACNNNGTG 1 cut(s) 329
DrdI GACNNNNNNGTC 1 cut(s) 86
DseDI GACNNNNNNGTC 1 cut(s) 86
EaeI YGGCCR 1 cut(s) 74
Eam1104I CTCTTC 2 cut(s) 692, 1329
EarI CTCTTC 2 cut(s) 692, 1329
EciI GGCGGA 1 cut(s) 12
Ecl136II GAGCTC 1 cut(s) 1074
Eco105I TACGTA 1 cut(s) 936
Eco130I CCWWGG 4 cut(s) 71, 520, 1224, 1679
Eco24I GRGCYC 2 cut(s) 806, 1076
Eco31I GGTCTC 1 cut(s) 708
Eco32I GATATC 1 cut(s) 163
Eco47I GGWCC 4 cut(s) 88, 133, 368, 1615
Eco47III AGCGCT 1 cut(s) 1810
Eco53kI GAGCTC 1 cut(s) 1074
Eco57I CTGAAG 1 cut(s) 278
EcoICRI GAGCTC 1 cut(s) 1074
EcoO109I RGGNCCY 4 cut(s) 133, 802, 803, 1615
EcoRI GAATTC 2 cut(s) 557, 1125
EcoRII CCWGG 2 cut(s) 114, 135
EcoRV GATATC 1 cut(s) 163
EcoT14I CCWWGG 4 cut(s) 71, 520, 1224, 1679
EcoT22I ATGCAT 1 cut(s) 1155
EcoT38I GRGCYC 2 cut(s) 806, 1076
ErhI CCWWGG 4 cut(s) 71, 520, 1224, 1679
FaeI CATG 9 cut(s) 75, 131, 524, 773, 1133, 1157, 1189, 1423, 1727
FaqI GGGAC 3 cut(s) 904, 1017, 1440
FatI CATG 9 cut(s) 71, 127, 520, 769, 1129, 1153, 1185, 1419, 1723
FbaI TGATCA 2 cut(s) 247, 460
FblI GTMKAC 1 cut(s) 682
Fnu4HI GCNGC 7 cut(s) 344, 540, 977, 1083, 1510, 1562, 1807
FokI GGATG 2 cut(s) 752, 1109
FriOI GRGCYC 2 cut(s) 806, 1076
Fsp4HI GCNGC 7 cut(s) 344, 540, 977, 1083, 1510, 1562, 1807
FspBI CTAG 5 cut(s) 503, 906, 1191, 1680, 1718
GlaI GCGC 3 cut(s) 238, 980, 1810
GluI GCNGC 7 cut(s) 344, 540, 977, 1083, 1510, 1562, 1807
GsuI CTGGAG 1 cut(s) 432
HaeII RGCGCY 2 cut(s) 982, 1812
HaeIII GGCC 4 cut(s) 76, 104, 519, 804
HapII CCGG 5 cut(s) 26, 77, 84, 91, 1433
HgaI GACGC 2 cut(s) 89, 756
HhaI GCGC 3 cut(s) 239, 981, 1811
Hin1I GRCGYC 2 cut(s) 81, 1141
Hin1II CATG 9 cut(s) 75, 131, 524, 773, 1133, 1157, 1189, 1423, 1727
Hin6I GCGC 3 cut(s) 237, 979, 1809
HinP1I GCGC 3 cut(s) 237, 979, 1809
HincII GTYRAC 1 cut(s) 1273
HindII GTYRAC 1 cut(s) 1273
HindIII AAGCTT 1 cut(s) 1686
HinfI GANTC 6 cut(s) 48, 668, 892, 1286, 1347, 1585
HpaII CCGG 5 cut(s) 26, 77, 84, 91, 1433
HphI GGTGA 3 cut(s) 366, 488, 1035
Hpy166II GTNNAC 5 cut(s) 125, 683, 1177, 1273, 1733
Hpy188III TCNNGA 9 cut(s) 503, 554, 580, 1130, 1433, 1439, 1484, 1520, 1795
Hpy8I GTNNAC 5 cut(s) 125, 683, 1177, 1273, 1733
HpyAV CCTTC 5 cut(s) 881, 937, 1127, 1517, 1606
HpyCH4III ACNGT 9 cut(s) 363, 613, 723, 846, 1277, 1298, 1506, 1593, 1642
HpyCH4IV ACGT 5 cut(s) 121, 935, 1037, 1141, 1802
HpyF10VI GCNNNNNNNGC 5 cut(s) 73, 236, 320, 536, 978
HpyF3I CTNAG 6 cut(s) 57, 326, 894, 1212, 1356, 1620
HpySE526I ACGT 5 cut(s) 121, 935, 1037, 1141, 1802
Hsp92I GRCGYC 2 cut(s) 81, 1141
Hsp92II CATG 9 cut(s) 75, 131, 524, 773, 1133, 1157, 1189, 1423, 1727
HspAI GCGC 3 cut(s) 237, 979, 1809
Kpn2I TCCGGA 1 cut(s) 1432
Ksp22I TGATCA 2 cut(s) 247, 460
Kzo9I GATC 8 cut(s) 195, 247, 331, 460, 499, 576, 1435, 1471
LmnI GCTCC 4 cut(s) 45, 308, 451, 1678
Lsp1109I GCAGC 4 cut(s) 355, 551, 1094, 1818
LweI GCATC 2 cut(s) 1069, 1768
MaeI CTAG 5 cut(s) 503, 906, 1191, 1680, 1718
MaeII ACGT 5 cut(s) 121, 935, 1037, 1141, 1802
MaeIII GTNAC 8 cut(s) 200, 271, 437, 484, 772, 788, 1239, 1292
MalI GATC 8 cut(s) 197, 249, 333, 462, 501, 578, 1437, 1473
MboI GATC 8 cut(s) 195, 247, 331, 460, 499, 576, 1435, 1471
MflI RGATCY 3 cut(s) 499, 1435, 1471
MhlI GDGCHC 4 cut(s) 127, 806, 1076, 1675
MluCI AATT 8 cut(s) 557, 569, 727, 1125, 1146, 1260, 1645, 1707
MlyI GAGTC 2 cut(s) 677, 886
MmeI TCCRAC 2 cut(s) 248, 443
Mph1103I ATGCAT 1 cut(s) 1155
MroI TCCGGA 1 cut(s) 1432
MroXI GAANNNNTTC 1 cut(s) 1742
MseI TTAA 5 cut(s) 141, 354, 605, 633, 999
MslI CAYNNNNRTG 3 cut(s) 759, 1335, 1728
MspA1I CMGCKG 2 cut(s) 230, 430
MspI CCGG 5 cut(s) 26, 77, 84, 91, 1433
MspR9I CCNGG 3 cut(s) 85, 116, 137
MvaI CCWGG 2 cut(s) 116, 137
MwoI GCNNNNNNNGC 5 cut(s) 73, 236, 320, 536, 978
NciI CCSGG 1 cut(s) 85
NcoI CCATGG 2 cut(s) 71, 520
NdeII GATC 8 cut(s) 195, 247, 331, 460, 499, 576, 1435, 1471
NlaIII CATG 9 cut(s) 75, 131, 524, 773, 1133, 1157, 1189, 1423, 1727
NlaIV GGNNCC 5 cut(s) 89, 804, 805, 1437, 1617
NmeAIII GCCGAG 1 cut(s) 153
NmuCI GTSAC 1 cut(s) 1239
NsiI ATGCAT 1 cut(s) 1155
NspI RCATGY 1 cut(s) 1157
PagI TCATGA 1 cut(s) 1129
PdmI GAANNNNTTC 1 cut(s) 1742
PfeI GAWTC 4 cut(s) 48, 1286, 1347, 1585
PflFI GACNNNGTC 1 cut(s) 1181
PkrI GCNGC 7 cut(s) 345, 541, 978, 1084, 1511, 1563, 1808
PleI GAGTC 2 cut(s) 676, 886
PpsI GAGTC 2 cut(s) 676, 886
Ppu21I YACGTR 1 cut(s) 936
PpuMI RGGWCCY 2 cut(s) 133, 1615
PshBI ATTAAT 1 cut(s) 354
PsiI TTATAA 3 cut(s) 1305, 1536, 1706
Psp124BI GAGCTC 1 cut(s) 1076
Psp5II RGGWCCY 2 cut(s) 133, 1615
Psp6I CCWGG 2 cut(s) 114, 135
PspGI CCWGG 2 cut(s) 114, 135
PspN4I GGNNCC 5 cut(s) 89, 804, 805, 1437, 1617
PspOMI GGGCCC 1 cut(s) 802
PspPI GGNCC 6 cut(s) 88, 133, 368, 802, 803, 1615
PspPPI RGGWCCY 2 cut(s) 133, 1615
PstI CTGCAG 1 cut(s) 1584
PstNI CAGNNNCTG 1 cut(s) 844
PsuI RGATCY 3 cut(s) 499, 1435, 1471
PsyI GACNNNGTC 1 cut(s) 1181
PvuII CAGCTG 1 cut(s) 230
RsaI GTAC 5 cut(s) 55, 191, 299, 550, 1734
RsaNI GTAC 5 cut(s) 54, 190, 298, 549, 1733
RseI CAYNNNNRTG 3 cut(s) 759, 1335, 1728
SacI GAGCTC 1 cut(s) 1076
SaqAI TTAA 5 cut(s) 141, 354, 605, 633, 999
SatI GCNGC 7 cut(s) 344, 540, 977, 1083, 1510, 1562, 1807
Sau3AI GATC 8 cut(s) 195, 247, 331, 460, 499, 576, 1435, 1471
Sau96I GGNCC 6 cut(s) 88, 133, 368, 802, 803, 1615
ScaI AGTACT 1 cut(s) 191
SchI GAGTC 2 cut(s) 677, 886
ScrFI CCNGG 3 cut(s) 85, 116, 137
SduI GDGCHC 4 cut(s) 127, 806, 1076, 1675
SfaNI GCATC 2 cut(s) 1069, 1768
SfcI CTRYAG 2 cut(s) 836, 1580
SgrAI CRCCGGYG 1 cut(s) 25
SinI GGWCC 4 cut(s) 88, 133, 368, 1615
SmiMI CAYNNNNRTG 3 cut(s) 759, 1335, 1728
SmlI CTYRAG 3 cut(s) 580, 1448, 1482
SmoI CTYRAG 3 cut(s) 580, 1448, 1482
SnaBI TACGTA 1 cut(s) 936
Sse9I AATT 8 cut(s) 557, 569, 727, 1125, 1146, 1260, 1645, 1707
SsiI CCGC 8 cut(s) 23, 177, 306, 428, 976, 1490, 1510, 1561
SspMI CTAG 5 cut(s) 503, 906, 1191, 1680, 1718
SstI GAGCTC 1 cut(s) 1076
StyD4I CCNGG 3 cut(s) 83, 114, 135
StyI CCWWGG 4 cut(s) 71, 520, 1224, 1679
TaaI ACNGT 9 cut(s) 363, 613, 723, 846, 1277, 1298, 1506, 1593, 1642
TaiI ACGT 5 cut(s) 124, 938, 1040, 1144, 1805
TaqI TCGA 2 cut(s) 732, 1588
TaqII GACCGA 1 cut(s) 732
TasI AATT 8 cut(s) 557, 569, 727, 1125, 1146, 1260, 1645, 1707
TatI WGTACW 3 cut(s) 53, 189, 297
TauI GCSGC 3 cut(s) 979, 1512, 1564
TfiI GAWTC 4 cut(s) 48, 1286, 1347, 1585
Tru1I TTAA 5 cut(s) 141, 354, 605, 633, 999
Tru9I TTAA 5 cut(s) 141, 354, 605, 633, 999
TscAI CASTG 4 cut(s) 334, 456, 1511, 1584
TseFI GTSAC 1 cut(s) 1239
TseI GCWGC 4 cut(s) 343, 539, 1082, 1806
Tsp45I GTSAC 1 cut(s) 1239
TspGWI ACGGA 2 cut(s) 32, 188
TspRI CASTG 4 cut(s) 334, 456, 1511, 1584
Tth111I GACNNNGTC 1 cut(s) 1181
VneI GTGCAC 1 cut(s) 123
VpaK11BI GGWCC 4 cut(s) 88, 133, 368, 1615
VspI ATTAAT 1 cut(s) 354
XapI RAATTY 3 cut(s) 557, 727, 1125
XbaI TCTAGA 1 cut(s) 502
XceI RCATGY 1 cut(s) 1157
XmaJI CCTAGG 1 cut(s) 1679
XmiI GTMKAC 1 cut(s) 682
XmnI GAANNNNTTC 1 cut(s) 1742
XspI CTAG 5 cut(s) 503, 906, 1191, 1680, 1718
ZraI GACGTC 1 cut(s) 1142
ZrmI AGTACT 1 cut(s) 191
Zsp2I ATGCAT 1 cut(s) 1155
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.