Rh2DG064000
ERF Family

Probably involved in the RNA silencing pathway and required for the generation of small interfering RNAs (siRNAs)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0000505
Physical Location & Seq
Forward (+)
4779720 .. 4782787
3068 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG064000.1

Sequence Viewer

Length: 846 bp
ATGCGTGTGCAGGAAAGCACTGGTCTTAGAAAAGGGAGACTTCATATTCCTGACTCTTATGTGTTGATGGGGACAGCTGATCCAACAGGGATTCTGGAAAGAGATGAAGTCTGCGTTATCCTTAAGGACGGACAAATGTCGGGGAAGGGACCACGTAATGTTGCTGATGAAATTGGTGGTGGAGATTTTGATGGTGATCTTTACTGGGTCTCAAGAAACCCTCAGCTATTGGAATGGTACAAACCAAGTGAACCTTGGAACGAAGGATCAATACCAACTCAAAAGGTTGCCAGTAATAGACCAACTGAGCTTTCCACTGAAGATCTTGAAGCTGATATGTTTAGATTGTTCTTGAAAAATAGGTTTCAACCAAGTTTTGCTATGAGTGAGATAGCTAATAACTGGCTAGCTTGGATAGATTGGTTCTTGACTTTGGGAGATAGTAATGAGAAGAAGGACGTAATGGACAAGATACTGAGGTTGGTTGATAAATACTACATCGCTCTAGATGCAGCGAAGAAAGGTGTAAAGGTTGAAGTTCCAAAAGATTTGAAGGTGTCACTTTTCCCTCATTACATGGACAAGAAAAAAGAAGAAACTTACATTTCATCTTCGATTTTGGGAGAAATTTATGATGAAGTTGATAAATGTCAAGCAAAAATAATTCTTCTCAAAGAAATCAAGAAACTTCCTATTTTTGATATTAATCAAGTCCCCGAACTCTGCTTGGCCAAATGGAAGAAACGTTATGGACGGTACAGGACTGAAATGAGCTCGGCCATGGATGAGGATGGAGATCAGGCTGCCATTGTCATAAGAAAGTATAAAAAGAAGCTGTATAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RdRP

Protein Analysis

281

Amino Acids

32.39

Weight (kDa)

6.78

Isoelectric Point (pI)

36.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RdRP PF05183 47 - 214 7.7e-28 RNA dependent RNA polymerase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000505)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G19910 AT2G19910 AT2G19920 AT2G19920 AT2G19920 AT2G19920 AT2G19930 AT2G19930 AT2G19930
fragaria_vesca FvH4_1g05980 FvH4_1g05990 FvH4_1g05990 FvH4_1g09718
malus_domestica MD02G1064400.v1.1 MD15G1195300.v1.1 MD15G1195400.v1.1
prunus_persica Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1
pyrus_communis pycom02g05070 pycom15g17460
rosa_chinensis RchiOBHm_Chr2g0091671 RchiOBHm_Chr2g0091681 RchiOBHm_Chr2g0091691 RchiOBHm_Chr2g0091701 RchiOBHm_Chr2g0091711 RchiOBHm_Chr2g0091721 RchiOBHm_Chr2g0091731 RchiOBHm_Chr5g0014401
rosa_laevigata RLG00000013772 RLG00000016242 RLG00000016244 RLG00000016245 RLG00000016246 RLG00000032105
rosa_multiflora Rmu_co8374041.1_g000001 Rmu_co8478121.1_g000001 Rmu_sc0000897.1_g000005 Rmu_sc0000897.1_g000009
rosa_roxburghii Rroxscaffold_1G00062180 Rroxscaffold_1G00062190 Rroxscaffold_1G00068800 Rroxscaffold_2G00149790 Rroxscaffold_2G00149800 Rroxscaffold_2G00149810
rosa_rugosa Rorug02G0019600 Rorug02G0019700 Rorug02G0019800 Rorug02G0019900 Rorug02G0019900 Rorug02G0020000 Rorug02G0020000 Rorug02G0020100 Rorug02G0020100 Rorug02G0020200 Rorug05G0017800
rosa_samantha Rh2AG064700 Rh2AG064800 Rh2AG064900 Rh2BG063800 Rh2BG063900 Rh2BG064000 Rh2BG064100 Rh2CG065500 Rh2CG065600 Rh2CG065700 Rh2DG063900 Rh2DG064000 Rh2DG064100 Rh2DG064200 Rh2DG381700 Rh5AG056700 Rh5AG056800 Rh5AG056900 Rh5AG112600 Rh5BG109300 Rh5CG063800 Rh5CG121100 Rh5DG053100 Rh5DG108300 Rh6CG100100
rosa_wichuraiana Rw2G005560 Rw2G005580 Rw2G005590 Rw5G005370 Rw5G009770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclI AACGTT 1 cut(s) 745
AclWI GGATC 2 cut(s) 74, 274
AcoI YGGCCR 2 cut(s) 729, 777
AcsI RAATTY 1 cut(s) 627
AcuI CTGAAG 1 cut(s) 339
AfaI GTAC 2 cut(s) 239, 758
AflII CTTAAG 1 cut(s) 122
AgsI TTSAA 5 cut(s) 329, 355, 368, 536, 553
AjuI GAANNNNNNNTTGG 2 cut(s) 295, 327
AluBI AGCT 8 cut(s) 77, 226, 310, 332, 395, 410, 774, 835
AluI AGCT 8 cut(s) 77, 226, 310, 332, 395, 410, 774, 835
Alw21I GWGCWC 1 cut(s) 776
Alw26I GTCTC 2 cut(s) 31, 214
AlwI GGATC 2 cut(s) 74, 274
AoxI GGCC 2 cut(s) 729, 777
ApeKI GCWGC 2 cut(s) 512, 803
ApoI RAATTY 1 cut(s) 627
AseI ATTAAT 1 cut(s) 705
AspS9I GGNCC 1 cut(s) 149
AsuHPI GGTGA 1 cut(s) 206
AsuNHI GCTAGC 1 cut(s) 406
AvaII GGWCC 1 cut(s) 149
BaeI ACNNNNGTAYC 2 cut(s) 464, 497
BalI TGGCCA 1 cut(s) 731
BanII GRGCYC 1 cut(s) 776
BarI GAAGNNNNNNTAC 2 cut(s) 255, 287
Bbv12I GWGCWC 1 cut(s) 776
BbvCI CCTCAGC 1 cut(s) 222
BbvI GCAGC 2 cut(s) 524, 790
BccI CCATC 3 cut(s) 61, 185, 785
BcoDI GTCTC 2 cut(s) 31, 214
BfaI CTAG 2 cut(s) 407, 506
BfrI CTTAAG 1 cut(s) 122
BglII AGATCT 1 cut(s) 322
BisI GCNGC 2 cut(s) 513, 804
BlsI GCNGC 2 cut(s) 514, 805
Bme18I GGWCC 1 cut(s) 149
BmgT120I GGNCC 1 cut(s) 149
BmiI GGNNCC 1 cut(s) 150
BmrI ACTGGG 1 cut(s) 214
BmsI GCATC 1 cut(s) 499
BmtI GCTAGC 1 cut(s) 410
BmuI ACTGGG 1 cut(s) 214
BoxI GACNNNNGTC 1 cut(s) 136
Bpu10I CCTNAGC 1 cut(s) 222
BpuEI CTTGAG 1 cut(s) 196
BsaAI YACGTR 1 cut(s) 155
BsaBI GATNNNNATC 3 cut(s) 195, 705, 795
BsaI GGTCTC 1 cut(s) 214
BsaJI CCNNGG 2 cut(s) 254, 780
Bse1I ACTGG 4 cut(s) 25, 209, 291, 407
Bse8I GATNNNNATC 3 cut(s) 195, 705, 795
BseDI CCNNGG 2 cut(s) 254, 780
BseGI GGATG 2 cut(s) 790, 796
BseJI GATNNNNATC 3 cut(s) 195, 705, 795
BseMII CTCAG 3 cut(s) 236, 297, 467
BseNI ACTGG 4 cut(s) 25, 209, 291, 407
BseXI GCAGC 2 cut(s) 524, 790
BsgI GTGCAG 1 cut(s) 29
BshFI GGCC 2 cut(s) 731, 779
BsiHKAI GWGCWC 1 cut(s) 776
BslFI GGGAC 3 cut(s) 85, 162, 698
BsmAI GTCTC 2 cut(s) 31, 214
BsmFI GGGAC 3 cut(s) 85, 162, 698
BsnI GGCC 2 cut(s) 731, 779
Bso31I GGTCTC 1 cut(s) 214
Bsp1286I GDGCHC 1 cut(s) 776
Bsp143I GATC 5 cut(s) 79, 196, 266, 322, 796
Bsp19I CCATGG 1 cut(s) 780
BspANI GGCC 2 cut(s) 731, 779
BspCNI CTCAG 3 cut(s) 235, 298, 468
BspLI GGNNCC 1 cut(s) 150
BspOI GCTAGC 1 cut(s) 410
BspPI GGATC 2 cut(s) 74, 274
BspTI CTTAAG 1 cut(s) 122
BspTNI GGTCTC 1 cut(s) 214
BsrI ACTGG 4 cut(s) 25, 209, 291, 407
BssECI CCNNGG 2 cut(s) 254, 780
BssMI GATC 5 cut(s) 79, 196, 266, 322, 796
BssT1I CCWWGG 2 cut(s) 254, 780
Bst4CI ACNGT 1 cut(s) 756
BstAFI CTTAAG 1 cut(s) 122
BstBAI YACGTR 1 cut(s) 155
BstC8I GCNNGC 1 cut(s) 408
BstDEI CTNAG 4 cut(s) 26, 222, 306, 476
BstDSI CCRYGG 1 cut(s) 780
BstF5I GGATG 2 cut(s) 790, 796
BstKTI GATC 5 cut(s) 82, 199, 269, 325, 799
BstMAI GTCTC 2 cut(s) 31, 214
BstMBI GATC 5 cut(s) 79, 196, 266, 322, 796
BstMWI GCNNNNNNNGC 1 cut(s) 509
BstPAI GACNNNNGTC 1 cut(s) 136
BstV1I GCAGC 2 cut(s) 524, 790
BstX2I RGATCY 1 cut(s) 322
BstYI RGATCY 1 cut(s) 322
BsuRI GGCC 2 cut(s) 731, 779
BtgI CCRYGG 1 cut(s) 780
BtgZI GCGATG 1 cut(s) 484
BtsCI GGATG 2 cut(s) 790, 796
BtsIMutI CAGTG 2 cut(s) 18, 315
Cac8I GCNNGC 1 cut(s) 408
Cfr13I GGNCC 1 cut(s) 149
Csp6I GTAC 2 cut(s) 238, 757
CviAII CATG 2 cut(s) 577, 781
CviQI GTAC 2 cut(s) 238, 757
DdeI CTNAG 4 cut(s) 26, 222, 306, 476
DpnI GATC 5 cut(s) 81, 198, 268, 324, 798
DpnII GATC 5 cut(s) 79, 196, 266, 322, 796
EaeI YGGCCR 2 cut(s) 729, 777
Ecl136II GAGCTC 1 cut(s) 774
Eco130I CCWWGG 2 cut(s) 254, 780
Eco24I GRGCYC 1 cut(s) 776
Eco31I GGTCTC 1 cut(s) 214
Eco47I GGWCC 1 cut(s) 149
Eco53kI GAGCTC 1 cut(s) 774
Eco57I CTGAAG 1 cut(s) 339
EcoICRI GAGCTC 1 cut(s) 774
EcoT14I CCWWGG 2 cut(s) 254, 780
EcoT38I GRGCYC 1 cut(s) 776
ErhI CCWWGG 2 cut(s) 254, 780
FaeI CATG 2 cut(s) 580, 784
FalI AAGNNNNNCTT 4 cut(s) 24, 56, 238, 270
FaqI GGGAC 3 cut(s) 85, 162, 698
FatI CATG 2 cut(s) 576, 780
Fnu4HI GCNGC 2 cut(s) 513, 804
FokI GGATG 2 cut(s) 797, 803
FriOI GRGCYC 1 cut(s) 776
Fsp4HI GCNGC 2 cut(s) 513, 804
FspBI CTAG 2 cut(s) 407, 506
GluI GCNGC 2 cut(s) 513, 804
HaeIII GGCC 2 cut(s) 731, 779
Hin1II CATG 2 cut(s) 580, 784
HinfI GANTC 2 cut(s) 53, 91
HphI GGTGA 1 cut(s) 206
Hpy166II GTNNAC 1 cut(s) 251
Hpy188III TCNNGA 8 cut(s) 50, 95, 213, 326, 352, 427, 506, 682
Hpy8I GTNNAC 1 cut(s) 251
HpyAV CCTTC 4 cut(s) 139, 257, 448, 547
HpyCH4III ACNGT 1 cut(s) 756
HpyCH4IV ACGT 3 cut(s) 154, 459, 745
HpyCH4V TGCA 2 cut(s) 10, 512
HpyF10VI GCNNNNNNNGC 1 cut(s) 509
HpyF3I CTNAG 4 cut(s) 26, 222, 306, 476
HpySE526I ACGT 3 cut(s) 154, 459, 745
Hsp92II CATG 2 cut(s) 580, 784
Kzo9I GATC 5 cut(s) 79, 196, 266, 322, 796
LpnPI CCDG 9 cut(s) 6, 63, 72, 80, 190, 304, 388, 745, 785
Lsp1109I GCAGC 2 cut(s) 524, 790
LweI GCATC 1 cut(s) 499
MaeI CTAG 2 cut(s) 407, 506
MaeII ACGT 3 cut(s) 154, 459, 745
MaeIII GTNAC 1 cut(s) 558
MalI GATC 5 cut(s) 81, 198, 268, 324, 798
MboI GATC 5 cut(s) 79, 196, 266, 322, 796
MboII GAAGA 7 cut(s) 332, 463, 529, 603, 605, 659, 751
MflI RGATCY 1 cut(s) 322
MhlI GDGCHC 1 cut(s) 776
MlsI TGGCCA 1 cut(s) 731
MluCI AATT 3 cut(s) 171, 627, 663
MluNI TGGCCA 1 cut(s) 731
MlyI GAGTC 1 cut(s) 47
MmeI TCCRAC 1 cut(s) 107
MnlI CCTC 4 cut(s) 231, 471, 579, 781
Mox20I TGGCCA 1 cut(s) 731
MscI TGGCCA 1 cut(s) 731
MseI TTAA 2 cut(s) 123, 705
Msp20I TGGCCA 1 cut(s) 731
MspA1I CMGCKG 1 cut(s) 77
MspCI CTTAAG 1 cut(s) 122
MwoI GCNNNNNNNGC 1 cut(s) 509
NcoI CCATGG 1 cut(s) 780
NdeII GATC 5 cut(s) 79, 196, 266, 322, 796
NheI GCTAGC 1 cut(s) 406
NlaIII CATG 2 cut(s) 580, 784
NlaIV GGNNCC 1 cut(s) 150
NmeAIII GCCGAG 1 cut(s) 755
NmuCI GTSAC 1 cut(s) 558
PfeI GAWTC 1 cut(s) 91
PkrI GCNGC 2 cut(s) 514, 805
PleI GAGTC 1 cut(s) 47
PpsI GAGTC 1 cut(s) 47
Ppu21I YACGTR 1 cut(s) 155
PshAI GACNNNNGTC 1 cut(s) 136
PshBI ATTAAT 1 cut(s) 705
Psp124BI GAGCTC 1 cut(s) 776
Psp1406I AACGTT 1 cut(s) 745
PspN4I GGNNCC 1 cut(s) 150
PspPI GGNCC 1 cut(s) 149
PsuI RGATCY 1 cut(s) 322
PvuII CAGCTG 1 cut(s) 77
RsaI GTAC 2 cut(s) 239, 758
RsaNI GTAC 2 cut(s) 238, 757
SacI GAGCTC 1 cut(s) 776
SaqAI TTAA 2 cut(s) 123, 705
SatI GCNGC 2 cut(s) 513, 804
Sau3AI GATC 5 cut(s) 79, 196, 266, 322, 796
Sau96I GGNCC 1 cut(s) 149
SchI GAGTC 1 cut(s) 47
SduI GDGCHC 1 cut(s) 776
SfaNI GCATC 1 cut(s) 499
SinI GGWCC 1 cut(s) 149
SmlI CTYRAG 2 cut(s) 122, 211
SmoI CTYRAG 2 cut(s) 122, 211
Sse9I AATT 3 cut(s) 171, 627, 663
SspMI CTAG 2 cut(s) 407, 506
SstI GAGCTC 1 cut(s) 776
StyI CCWWGG 2 cut(s) 254, 780
TaaI ACNGT 1 cut(s) 756
TaiI ACGT 3 cut(s) 157, 462, 748
TaqI TCGA 1 cut(s) 614
TasI AATT 3 cut(s) 171, 627, 663
TfiI GAWTC 1 cut(s) 91
Tru1I TTAA 2 cut(s) 123, 705
Tru9I TTAA 2 cut(s) 123, 705
TscAI CASTG 2 cut(s) 25, 322
TseFI GTSAC 1 cut(s) 558
TseI GCWGC 2 cut(s) 512, 803
Tsp45I GTSAC 1 cut(s) 558
TspDTI ATGAA 5 cut(s) 32, 120, 183, 597, 651
TspGWI ACGGA 1 cut(s) 144
TspRI CASTG 2 cut(s) 25, 322
Vha464I CTTAAG 1 cut(s) 122
VpaK11BI GGWCC 1 cut(s) 149
VspI ATTAAT 1 cut(s) 705
XapI RAATTY 1 cut(s) 627
XbaI TCTAGA 1 cut(s) 505
XcmI CCANNNNNNNNNTGG 1 cut(s) 252
XspI CTAG 2 cut(s) 407, 506
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.