Rh2AG064900
ERF Family

Probably involved in the RNA silencing pathway and required for the generation of small interfering RNAs (siRNAs)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0000505
Physical Location & Seq
Forward (+)
5382529 .. 5394444
11916 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG064900.1

Sequence Viewer

Length: 3237 bp
ATGTCCGGCGTGCAGCTACTTGACTCGGTGCAGCAATCGCTGGATCGAATATGCCACCTTAAAAACCAACCACAGCCGAACGACGGCATCAGAAGAAAGCTTGCATTGCTGAGCGAGCAAGACGCCCTCTACGTGCTAAACCAAATTTACAACACAAAAAATCCCATCAGAACCCTCAATGGATACATCTCTTTCATGATCAAGGAACTAGAGAATGAATCTTCTTCGTCGTCTTCAAGAACAGTTTGGACCCCTCAGAGTCCCTCTGCTTCTTCTTCTTCTTCATTTACTAGTCCTGCACGGCCTGATCATTCTCAAGGTGAGACAATCCAGTGTGGTTCTCCTGTTAATCGACAGCCACTCTATTCTTCAGCTTCCCCGACTTCTTCTTATCAGAGCCATTCCTCTCCTACTATTACTGATGCATCATTTTATTCCAGTTCTCAGCCTCAGGATGAGACTCCCAACCCATCCCCGGGGACAGTGTGCAATGGACAATCACCCTATTCCTTCCCTTCTCTCTATCAAACCCTATCTGCTCCTTTTAATACTCTTAGACAACTCTATGAGTGGTTCCGAGGTGAGACTGTCACTCAATCCCCAGTTCTTTACCATCCTGGAGACCATGGACCAGCACACTTAGAAGCTCTGGGAGAACTTGATTTCAGAAAGCAATTTCTGATTTTGAGTTATGCTGGAGGAACGAAGTTAAAAGATGTAATAGAAGCGGAGGCTATCAGGAGCTGGAAAAGTTTGCCAATGGTAAAGTTCGAGACCACAGTTTGGCATGCTTTGGGTGAAAACAATGTTGGCAAGGCTGACCGACGATTGTATTCTGATTGGGATTCTCAAAGAACACACCAGTACTATTGTTATGTTTCGGTGGATGGGAGTTACAGTTTCAAGGGTCCCTTCCTAGACAAACCAAGAACTCTCCTGCAGAAACAGTTAGGAGATGACAATGTTTTGGAGGTGAAATTTACCCAATTGGAAGGTTCCAGTGGTACATATAGCAAGTTAATGCGAGAAGGCATTCTTGTTGGTTCGCGGCGATACGCTTTCTTTGTGTTCCGTGCTGGTAACAAAGGAAAGAATATGTGGAAAGACCCAACTTCATCACCTGTGAAATGTTACTTCGTTCGTGGGAGCTCTGATGCAGTGATAGACAGGGGTATAGGCTATAAGTTATCTGGCAAAACGATGCATGAAGGAAGATCTCTTTTCACGCATGCACACACTCGGCCTAGTTTGGCCAGCTACATGGATAGGTTATCTCTCATTTTGTCCAAGACAGTTACTTTAGAAGTAAATTGGTCACATGTCAAGGTGGAATTCATCAAAGACATAGACTGTCGCGATGAGTCTGGTAATCCCATCTATGTAGATGGAAAACCTCGCATACATACTGATGGCACAGGATTTATATCTGAGGATCTGGCTTTACTTTGTCCTGAGATAGCACGCAAAGGAGAGCAGATCAATAGTGAACACATCGAGGGACTTGATGATCCTGATGATGAACTTGAGGACAAAATTCTGGAATGGAAACAGCCAGGCACTAGAACTCGGGAACTGCCTTTGCTCATGCAGTTCCGACTTTTTTACGAAGGCCGTGCTATCAAAGGAACTTTTCTTGTCAATAAAGAGCTTCCACCAAAAACTTTGAAGATTCGATCATCAATGGTTAAAGTTGAGCCAGATCCAAAATTGTCAGATCCTCAAACAGTGAATTCACTGGAGATAGTAGGAACAAGTAAACTTAATAAGAGAACGTATTTGTCGAGGAACTTAATTGCACTTTTGTACCATGGAGGGGTGCCAAAAAAATACTTCATCGAGTTACTTATGAAAGCTTTAGAAGAGACTCGTGATGTTTTTAGCAATCCACGGGCAGCATTCAGAGTTGCCCTTAGATATGGCGAGATCGATGACAACAGTACTGTTGCGAAAATGATCTCCTGTGGTATTCCTCTTGAAGAACCATATGTGCAATATCGTCTTTCAACCCTTATGAACATGGAAAAGATCAATCTCAAAAAAGGGAAAATTTGTATTCCTGAATCATACTATTTGATGGGGACAGCTGATCCAACAGGAAAACTTGAAAAAGATGAAGTTTGCATTATCCTAGAAAATGGACAACTACTGTCGGGAGATTTTCTAGTGTACCGGAATCCTGGTTTACACTTCGGTGATATTCATGTCCTAAAGGCCAGATATGTCAAGGAACTAGAATCCGTTGTTGGCAATGCCAAGTACGGTATATTCTTCTCTAGTAAAGGTCCACGTTCCGTTGCTGATGAAATGGCTGGTGGAGATTTTGATGGTGATCTATACTGGATCTCAAGAAACCCTCAGCTATTAAAATCGTTTAAACCAAGTGAACCTTGGATTGGAAAGTCATCTGCCCAAAAAGTTCATGGTAAAAGACCAACCGAGCTTTCAAAGGATGATCTAGAAGATGAGCTCATCAAACTATTCTTAAAGACTAGGTTTGAACCAAGCTTCGCAGTGTATGAGGCAGCTCAAAACTGGATGGCTTTGATGGATAAATTTTTGACTCTAGGAGATACGAGTATTGATGTGAAAAACAAACTGAAGGATAAGATATTGCGATTGATTGATATTTACTATGAAGCTCTCGATGCACCAAAAAAAGGTTTTGAGGTTGTAGTTCCAAAAGAATTGAAGGTGCGAGAGTTCCCACATTTCATGGAGAAGAAAAACCAAGAATCCTACCATTCAAGTTCCATTTTGGGATCCATTTATGATAAGGTGGAGGAATATGAAACAGAAGACAATCTTAAAGAAATTCGGAAACTTCCTCTTTTTGAAGACGACGTTCCTGAGGACCGCTTGAAAGAATGGGAGAAACGATACAAAGAGTACCGGAAGAAAATGACCTCTGCCATGGGATATGAAGACAAAGAGAGCAAGAATCAGGCAGCCAATATCGTCATAAGAGAGTATAAAGAGATCCTATACGAGGCTGAGGAGTTTGAGGAAAGTAAAAGGGACAGACAACAAATATATAGCGACGCACTCGCTATTTATCATGTTTGTTATGACTATGCCAGGAGTGTGAGGACTGAAACTGGGGCACAAGATGTCCGGAAATGTGGCTTTGTTTGGAAAGTTGCAGGTGCAGCTCTATGCCAGCTTCATGAGGACCGGAGTTTGAAACCTGGTGAAAAGGTTTTCAGGATCTTACCCTCCGTTTTGAAGGACATTATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RdRP

Protein Analysis

1078

Amino Acids

122.68

Weight (kDa)

6.78

Isoelectric Point (pI)

40.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
4HB_RdRP3_N PF26249 6 - 69 6.2e-08 RdRP3/4/5, N-terminal four helical bundle domain
RdRP_helical PF26252 213 - 277 4.4e-08 RNA dependent RNA polymerase, helical domain
RdRP PF05183 300 - 926 3.1e-102 RNA dependent RNA polymerase
RdRP_head PF26253 959 - 1057 1.8e-12 RdRP, head domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000505)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G19910 AT2G19910 AT2G19920 AT2G19920 AT2G19920 AT2G19920 AT2G19930 AT2G19930 AT2G19930
fragaria_vesca FvH4_1g05980 FvH4_1g05990 FvH4_1g05990 FvH4_1g09718
malus_domestica MD02G1064400.v1.1 MD15G1195300.v1.1 MD15G1195400.v1.1
prunus_persica Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1
pyrus_communis pycom02g05070 pycom15g17460
rosa_chinensis RchiOBHm_Chr2g0091671 RchiOBHm_Chr2g0091681 RchiOBHm_Chr2g0091691 RchiOBHm_Chr2g0091701 RchiOBHm_Chr2g0091711 RchiOBHm_Chr2g0091721 RchiOBHm_Chr2g0091731 RchiOBHm_Chr5g0014401
rosa_laevigata RLG00000013772 RLG00000016242 RLG00000016244 RLG00000016245 RLG00000016246 RLG00000032105
rosa_multiflora Rmu_co8374041.1_g000001 Rmu_co8478121.1_g000001 Rmu_sc0000897.1_g000005 Rmu_sc0000897.1_g000009
rosa_roxburghii Rroxscaffold_1G00062180 Rroxscaffold_1G00062190 Rroxscaffold_1G00068800 Rroxscaffold_2G00149790 Rroxscaffold_2G00149800 Rroxscaffold_2G00149810
rosa_rugosa Rorug02G0019600 Rorug02G0019700 Rorug02G0019800 Rorug02G0019900 Rorug02G0019900 Rorug02G0020000 Rorug02G0020000 Rorug02G0020100 Rorug02G0020100 Rorug02G0020200 Rorug05G0017800
rosa_samantha Rh2AG064700 Rh2AG064800 Rh2AG064900 Rh2BG063800 Rh2BG063900 Rh2BG064000 Rh2BG064100 Rh2CG065500 Rh2CG065600 Rh2CG065700 Rh2DG063900 Rh2DG064000 Rh2DG064100 Rh2DG064200 Rh2DG381700 Rh5AG056700 Rh5AG056800 Rh5AG056900 Rh5AG112600 Rh5BG109300 Rh5CG063800 Rh5CG121100 Rh5DG053100 Rh5DG108300 Rh6CG100100
rosa_wichuraiana Rw2G005560 Rw2G005580 Rw2G005590 Rw5G005370 Rw5G009770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 3131
Acc36I ACCTGC 1 cut(s) 3131
AccB1I GGYRCC 1 cut(s) 1816
AccB7I CCANNNNNTGG 1 cut(s) 783
AccII CGCG 2 cut(s) 1048, 1356
AccIII TCCGGA 1 cut(s) 3111
AciI CCGC 3 cut(s) 728, 1048, 2854
AcoI YGGCCR 1 cut(s) 1251
AcsI RAATTY 8 cut(s) 144, 977, 1331, 1533, 1729, 2046, 2552, 2811
AcuI CTGAAG 2 cut(s) 354, 2618
AcyI GRCGYC 1 cut(s) 123
AfaI GTAC 7 cut(s) 866, 1006, 1805, 1939, 2168, 2258, 2888
AfiI CCNNNNNNNGG 8 cut(s) 83, 475, 476, 783, 1813, 2393, 2657, 2986
AflIII ACRYGT 1 cut(s) 1318
AhlI ACTAGT 1 cut(s) 290
AjnI CCWGG 5 cut(s) 616, 1552, 2176, 3074, 3184
AjuI GAANNNNNNNTTGG 8 cut(s) 792, 824, 2226, 2258, 2376, 2408, 2425, 2457
AleI CACNNNNGTG 1 cut(s) 2190
Alw21I GWGCWC 2 cut(s) 1151, 2469
Alw26I GTCTC 6 cut(s) 317, 452, 578, 615, 767, 1856
AlwNI CAGNNNCTG 1 cut(s) 744
Ama87I CYCGRG 2 cut(s) 475, 1566
Aor13HI TCCGGA 1 cut(s) 3111
AoxI GGCC 5 cut(s) 302, 1241, 1251, 1609, 2211
ApeKI GCWGC 6 cut(s) 13, 31, 1892, 2522, 2945, 3146
ApoI RAATTY 8 cut(s) 144, 977, 1331, 1533, 1729, 2046, 2552, 2811
Asp700I GAANNNNTTC 1 cut(s) 1032
AspS9I GGNCC 6 cut(s) 249, 629, 908, 2282, 2851, 3169
AsuC2I CCSGG 2 cut(s) 476, 477
AsuHPI GGTGA 9 cut(s) 332, 492, 593, 809, 985, 1110, 2204, 2339, 3200
AvaI CYCGRG 2 cut(s) 475, 1566
AvaII GGWCC 6 cut(s) 249, 629, 908, 2282, 2851, 3169
AxyI CCTNAGG 2 cut(s) 450, 2847
BaeGI GKGCMC 1 cut(s) 3103
BaeI ACNNNNGTAYC 2 cut(s) 1787, 1820
BalI TGGCCA 1 cut(s) 1253
BamHI GGATCC 1 cut(s) 2759
BanI GGYRCC 1 cut(s) 1816
BanII GRGCYC 2 cut(s) 1151, 2469
BauI CACGAG 1 cut(s) 1866
BbsI GAAGAC 4 cut(s) 225, 2802, 2841, 2928
Bbv12I GWGCWC 2 cut(s) 1151, 2469
BbvCI CCTCAGC 2 cut(s) 2355, 2991
BbvI GCAGC 6 cut(s) 25, 43, 1904, 2534, 2957, 3158
BceAI ACGGC 3 cut(s) 100, 317, 1596
BcgI CGANNNNNNTGC 2 cut(s) 1595, 1629
BciT130I CCWGG 5 cut(s) 618, 1554, 2178, 3076, 3186
BciVI GTATCC 1 cut(s) 176
BclI TGATCA 2 cut(s) 198, 307
BcnI CCSGG 2 cut(s) 476, 477
BcoDI GTCTC 6 cut(s) 317, 452, 578, 615, 767, 1856
BcuI ACTAGT 1 cut(s) 290
BfmI CTRYAG 1 cut(s) 938
BfuAI ACCTGC 1 cut(s) 3131
BfuI GTATCC 1 cut(s) 176
BglII AGATCT 1 cut(s) 1214
BisI GCNGC 7 cut(s) 14, 32, 1049, 1893, 2523, 2946, 3147
BlpI GCTNAGC 1 cut(s) 110
BlsI GCNGC 7 cut(s) 15, 33, 1050, 1894, 2524, 2947, 3148
BmcAI AGTACT 2 cut(s) 866, 1939
Bme1390I CCNGG 7 cut(s) 476, 477, 618, 1554, 2178, 3076, 3186
Bme18I GGWCC 6 cut(s) 249, 629, 908, 2282, 2851, 3169
BmeT110I CYCGRG 2 cut(s) 475, 1566
BmgT120I GGNCC 6 cut(s) 249, 629, 908, 2282, 2851, 3169
BmiI GGNNCC 7 cut(s) 251, 575, 909, 910, 997, 1818, 2761
BmrFI CCNGG 7 cut(s) 476, 477, 618, 1554, 2178, 3076, 3186
BmrI ACTGGG 2 cut(s) 596, 3105
BmsI GCATC 6 cut(s) 96, 412, 434, 1144, 1191, 2635
BmuI ACTGGG 2 cut(s) 596, 3105
BpiI GAAGAC 4 cut(s) 225, 2802, 2841, 2928
BpmI CTGGAG 3 cut(s) 639, 717, 1757
Bpu10I CCTNAGC 2 cut(s) 2355, 2991
Bpu1102I GCTNAGC 1 cut(s) 110
BpuEI CTTGAG 3 cut(s) 300, 1544, 2329
BpuMI CCSGG 2 cut(s) 476, 477
Bsa29I ATCGAT 1 cut(s) 1926
BsaAI YACGTR 1 cut(s) 133
BsaBI GATNNNNATC 2 cut(s) 1423, 2328
BsaHI GRCGYC 1 cut(s) 123
BsaI GGTCTC 2 cut(s) 615, 767
BsaJI CCNNGG 9 cut(s) 474, 475, 476, 577, 625, 1807, 1886, 2387, 2910
BsaWI WCCGGW 4 cut(s) 2169, 2889, 3111, 3171
BsaXI ACNNNNNCTCC 2 cut(s) 883, 913
Bsc4I CCNNNNNNNGG 8 cut(s) 83, 475, 476, 783, 1813, 2393, 2657, 2986
Bse1I ACTGG 9 cut(s) 331, 438, 602, 862, 999, 1740, 2342, 2537, 3100
Bse21I CCTNAGG 2 cut(s) 450, 2847
Bse3DI GCAATG 3 cut(s) 104, 496, 2254
Bse8I GATNNNNATC 2 cut(s) 1423, 2328
BseAI TCCGGA 1 cut(s) 3111
BseBI CCWGG 5 cut(s) 618, 1554, 2178, 3076, 3186
BseCI ATCGAT 1 cut(s) 1926
BseDI CCNNGG 9 cut(s) 474, 475, 476, 577, 625, 1807, 1886, 2387, 2910
BseGI GGATG 6 cut(s) 460, 470, 613, 892, 2455, 2541
BseJI GATNNNNATC 2 cut(s) 1423, 2328
BseLI CCNNNNNNNGG 8 cut(s) 83, 475, 476, 783, 1813, 2393, 2657, 2986
BseMI GCAATG 3 cut(s) 104, 496, 2254
BseMII CTCAG 9 cut(s) 101, 269, 458, 464, 1419, 1443, 2369, 2838, 2982
BseNI ACTGG 9 cut(s) 331, 438, 602, 862, 999, 1740, 2342, 2537, 3100
BseRI GAGGAG 1 cut(s) 3008
BseSI GKGCMC 1 cut(s) 3103
BseXI GCAGC 6 cut(s) 25, 43, 1904, 2534, 2957, 3158
BsgI GTGCAG 4 cut(s) 32, 50, 282, 3165
Bsh1236I CGCG 2 cut(s) 1048, 1356
BshFI GGCC 5 cut(s) 304, 1243, 1253, 1611, 2213
BshNI GGYRCC 1 cut(s) 1816
BshVI ATCGAT 1 cut(s) 1926
BsiHKAI GWGCWC 2 cut(s) 1151, 2469
BsiHKCI CYCGRG 2 cut(s) 475, 1566
BsiSI CCGG 6 cut(s) 6, 476, 2170, 2890, 3112, 3172
BslFI GGGAC 6 cut(s) 246, 493, 894, 1512, 2092, 3029
BslI CCNNNNNNNGG 8 cut(s) 83, 475, 476, 783, 1813, 2393, 2657, 2986
BsmAI GTCTC 6 cut(s) 317, 452, 578, 615, 767, 1856
BsmFI GGGAC 6 cut(s) 246, 493, 894, 1512, 2092, 3029
BsmI GAATGC 2 cut(s) 1032, 1895
BsnI GGCC 5 cut(s) 304, 1243, 1253, 1611, 2213
Bso31I GGTCTC 2 cut(s) 615, 767
BsoBI CYCGRG 2 cut(s) 475, 1566
Bsp1286I GDGCHC 3 cut(s) 1151, 2469, 3103
Bsp13I TCCGGA 1 cut(s) 3111
Bsp1720I GCTNAGC 1 cut(s) 110
Bsp19I CCATGG 3 cut(s) 625, 1807, 2910
Bsp68I TCGCGA 1 cut(s) 1356
BspACI CCGC 3 cut(s) 728, 1048, 2854
BspANI GGCC 5 cut(s) 304, 1243, 1253, 1611, 2213
BspCNI CTCAG 9 cut(s) 102, 268, 457, 463, 1420, 1444, 2368, 2839, 2983
BspDI ATCGAT 1 cut(s) 1926
BspEI TCCGGA 1 cut(s) 3111
BspFNI CGCG 2 cut(s) 1048, 1356
BspHI TCATGA 2 cut(s) 195, 3163
BspLI GGNNCC 7 cut(s) 251, 575, 909, 910, 997, 1818, 2761
BspMAI CTGCAG 1 cut(s) 942
BspMI ACCTGC 1 cut(s) 3131
BspT107I GGYRCC 1 cut(s) 1816
BspTNI GGTCTC 2 cut(s) 615, 767
BsrDI GCAATG 3 cut(s) 104, 496, 2254
BsrI ACTGG 9 cut(s) 331, 438, 602, 862, 999, 1740, 2342, 2537, 3100
BssECI CCNNGG 9 cut(s) 474, 475, 476, 577, 625, 1807, 1886, 2387, 2910
BssNI GRCGYC 1 cut(s) 123
BssSI CACGAG 1 cut(s) 1866
BssT1I CCWWGG 4 cut(s) 625, 1807, 2387, 2910
Bst2BI CACGAG 1 cut(s) 1866
Bst2UI CCWGG 5 cut(s) 618, 1554, 2178, 3076, 3186
Bst6I CTCTTC 1 cut(s) 1854
BstACI GRCGYC 1 cut(s) 123
BstBAI YACGTR 1 cut(s) 133
BstC8I GCNNGC 8 cut(s) 11, 102, 116, 789, 1230, 1255, 1462, 3158
BstDSI CCRYGG 4 cut(s) 625, 1807, 1886, 2910
BstENI CCTNNNNNAGG 1 cut(s) 2984
BstF5I GGATG 6 cut(s) 460, 470, 613, 892, 2455, 2541
BstFNI CGCG 2 cut(s) 1048, 1356
BstMAI GTCTC 6 cut(s) 317, 452, 578, 615, 767, 1856
BstMWI GCNNNNNNNGC 5 cut(s) 37, 106, 115, 2645, 3146
BstNI CCWGG 5 cut(s) 618, 1554, 2178, 3076, 3186
BstNSI RCATGY 3 cut(s) 791, 1232, 1322
BstSCI CCNGG 7 cut(s) 474, 475, 616, 1552, 2176, 3074, 3184
BstSFI CTRYAG 1 cut(s) 938
BstSLI GKGCMC 1 cut(s) 3103
BstUI CGCG 2 cut(s) 1048, 1356
BstV1I GCAGC 6 cut(s) 25, 43, 1904, 2534, 2957, 3158
BstV2I GAAGAC 4 cut(s) 225, 2802, 2841, 2928
BstX2I RGATCY 8 cut(s) 1214, 1432, 1699, 1714, 2340, 2759, 2976, 3204
BstXI CCANNNNNNTGG 1 cut(s) 1261
BstYI RGATCY 8 cut(s) 1214, 1432, 1699, 1714, 2340, 2759, 2976, 3204
Bsu15I ATCGAT 1 cut(s) 1926
Bsu36I CCTNAGG 2 cut(s) 450, 2847
BsuI GTATCC 1 cut(s) 176
BsuRI GGCC 5 cut(s) 304, 1243, 1253, 1611, 2213
BsuTUI ATCGAT 1 cut(s) 1926
BtgI CCRYGG 4 cut(s) 625, 1807, 1886, 2910
BtgZI GCGATG 1 cut(s) 1371
BtsCI GGATG 6 cut(s) 460, 470, 613, 892, 2455, 2541
BtsI GCAGTG 2 cut(s) 1164, 2517
BtsIMutI CAGTG 7 cut(s) 338, 489, 1006, 1164, 1731, 1733, 2517
BtuMI TCGCGA 1 cut(s) 1356
BveI ACCTGC 1 cut(s) 3131
Cac8I GCNNGC 8 cut(s) 11, 102, 116, 789, 1230, 1255, 1462, 3158
CaiI CAGNNNCTG 1 cut(s) 744
CciI TCATGA 2 cut(s) 195, 3163
Cfr13I GGNCC 6 cut(s) 249, 629, 908, 2282, 2851, 3169
Cfr9I CCCGGG 1 cut(s) 475
ClaI ATCGAT 1 cut(s) 1926
CseI GACGC 2 cut(s) 131, 3047
CsiI ACCWGGT 1 cut(s) 3184
Csp6I GTAC 7 cut(s) 865, 1005, 1804, 1938, 2167, 2257, 2887
CviQI GTAC 7 cut(s) 865, 1005, 1804, 1938, 2167, 2257, 2887
DraI TTTAAA 1 cut(s) 2374
EaeI YGGCCR 1 cut(s) 1251
Eam1104I CTCTTC 1 cut(s) 1854
EarI CTCTTC 1 cut(s) 1854
Ecl136II GAGCTC 2 cut(s) 1149, 2467
Eco130I CCWWGG 4 cut(s) 625, 1807, 2387, 2910
Eco24I GRGCYC 2 cut(s) 1151, 2469
Eco31I GGTCTC 2 cut(s) 615, 767
Eco47I GGWCC 6 cut(s) 249, 629, 908, 2282, 2851, 3169
Eco53kI GAGCTC 2 cut(s) 1149, 2467
Eco57I CTGAAG 2 cut(s) 354, 2618
Eco81I CCTNAGG 2 cut(s) 450, 2847
Eco88I CYCGRG 2 cut(s) 475, 1566
EcoICRI GAGCTC 2 cut(s) 1149, 2467
EcoNI CCTNNNNNAGG 1 cut(s) 2984
EcoO109I RGGNCCY 1 cut(s) 908
EcoRI GAATTC 2 cut(s) 1331, 1729
EcoRII CCWGG 5 cut(s) 616, 1552, 2176, 3074, 3184
EcoT14I CCWWGG 4 cut(s) 625, 1807, 2387, 2910
EcoT22I ATGCAT 2 cut(s) 427, 1206
EcoT38I GRGCYC 2 cut(s) 1151, 2469
ErhI CCWWGG 4 cut(s) 625, 1807, 2387, 2910
FalI AAGNNNNNCTT 8 cut(s) 896, 928, 1020, 1052, 2371, 2403, 2787, 2819
FaqI GGGAC 6 cut(s) 246, 493, 894, 1512, 2092, 3029
FauNDI CATATG 1 cut(s) 1984
FbaI TGATCA 2 cut(s) 198, 307
Fnu4HI GCNGC 7 cut(s) 14, 32, 1049, 1893, 2523, 2946, 3147
FokI GGATG 6 cut(s) 457, 467, 600, 899, 2462, 2548
FriOI GRGCYC 2 cut(s) 1151, 2469
Fsp4HI GCNGC 7 cut(s) 14, 32, 1049, 1893, 2523, 2946, 3147
GluI GCNGC 7 cut(s) 14, 32, 1049, 1893, 2523, 2946, 3147
GsuI CTGGAG 3 cut(s) 639, 717, 1757
HaeIII GGCC 5 cut(s) 304, 1243, 1253, 1611, 2213
HapII CCGG 6 cut(s) 6, 476, 2170, 2890, 3112, 3172
HgaI GACGC 2 cut(s) 131, 3047
Hin1I GRCGYC 1 cut(s) 123
HindIII AAGCTT 3 cut(s) 98, 1851, 2503
HpaII CCGG 6 cut(s) 6, 476, 2170, 2890, 3112, 3172
HphI GGTGA 9 cut(s) 332, 492, 593, 809, 985, 1110, 2204, 2339, 3200
Hpy166II GTNNAC 6 cut(s) 1487, 1757, 2167, 2183, 2285, 2384
Hpy8I GTNNAC 6 cut(s) 1487, 1757, 2167, 2183, 2285, 2384
Hpy99I CGWCG 5 cut(s) 86, 232, 828, 2843, 3041
HpyCH4IV ACGT 4 cut(s) 132, 1772, 2287, 2841
HpyF10VI GCNNNNNNNGC 5 cut(s) 37, 106, 115, 2645, 3146
HpySE526I ACGT 4 cut(s) 132, 1772, 2287, 2841
Hsp92I GRCGYC 1 cut(s) 123
KflI GGGWCCC 1 cut(s) 908
Kpn2I TCCGGA 1 cut(s) 3111
Ksp22I TGATCA 2 cut(s) 198, 307
LmnI GCTCC 3 cut(s) 544, 741, 1146
Lsp1109I GCAGC 6 cut(s) 25, 43, 1904, 2534, 2957, 3158
LweI GCATC 6 cut(s) 96, 412, 434, 1144, 1191, 2635
MabI ACCWGGT 1 cut(s) 3184
MaeII ACGT 4 cut(s) 132, 1772, 2287, 2841
MaeIII GTNAC 7 cut(s) 589, 893, 1079, 1130, 1294, 1314, 1839
MfeI CAATTG 1 cut(s) 986
MflI RGATCY 8 cut(s) 1214, 1432, 1699, 1714, 2340, 2759, 2976, 3204
MhlI GDGCHC 3 cut(s) 1151, 2469, 3103
MlsI TGGCCA 1 cut(s) 1253
MluNI TGGCCA 1 cut(s) 1253
MlyI GAGTC 6 cut(s) 17, 268, 454, 1370, 1858, 2554
MmeI TCCRAC 2 cut(s) 1618, 2114
Mox20I TGGCCA 1 cut(s) 1253
Mph1103I ATGCAT 2 cut(s) 427, 1206
MroI TCCGGA 1 cut(s) 3111
MroXI GAANNNNTTC 1 cut(s) 1032
MscI TGGCCA 1 cut(s) 1253
MslI CAYNNNNRTG 2 cut(s) 1407, 2190
Msp20I TGGCCA 1 cut(s) 1253
MspA1I CMGCKG 1 cut(s) 2084
MspI CCGG 6 cut(s) 6, 476, 2170, 2890, 3112, 3172
MspR9I CCNGG 7 cut(s) 476, 477, 618, 1554, 2178, 3076, 3186
MssI GTTTAAAC 1 cut(s) 2374
MunI CAATTG 1 cut(s) 986
Mva1269I GAATGC 2 cut(s) 1032, 1895
MvaI CCWGG 5 cut(s) 618, 1554, 2178, 3076, 3186
MvnI CGCG 2 cut(s) 1048, 1356
MwoI GCNNNNNNNGC 5 cut(s) 37, 106, 115, 2645, 3146
NciI CCSGG 2 cut(s) 476, 477
NcoI CCATGG 3 cut(s) 625, 1807, 2910
NdeI CATATG 1 cut(s) 1984
NlaIV GGNNCC 7 cut(s) 251, 575, 909, 910, 997, 1818, 2761
NmeAIII GCCGAG 1 cut(s) 1219
NmuCI GTSAC 2 cut(s) 589, 1314
NruI TCGCGA 1 cut(s) 1356
NsiI ATGCAT 2 cut(s) 427, 1206
NspI RCATGY 3 cut(s) 791, 1232, 1322
OliI CACNNNNGTG 1 cut(s) 2190
PaeI GCATGC 2 cut(s) 791, 1232
PagI TCATGA 2 cut(s) 195, 3163
PaqCI CACCTGC 1 cut(s) 3131
PciI ACATGT 1 cut(s) 1318
PcsI WCGNNNNNNNCGW 2 cut(s) 129, 1778
PctI GAATGC 2 cut(s) 1032, 1895
PdmI GAANNNNTTC 1 cut(s) 1032
PfeI GAWTC 8 cut(s) 218, 845, 1669, 2060, 2173, 2234, 2732, 2938
PflMI CCANNNNNTGG 1 cut(s) 783
PfoI TCCNGGA 1 cut(s) 616
PkrI GCNGC 7 cut(s) 15, 33, 1050, 1894, 2524, 2947, 3148
PleI GAGTC 6 cut(s) 17, 267, 454, 1369, 1858, 2554
PmeI GTTTAAAC 1 cut(s) 2374
PpsI GAGTC 6 cut(s) 17, 267, 454, 1369, 1858, 2554
Ppu21I YACGTR 1 cut(s) 133
PpuMI RGGWCCY 1 cut(s) 908
PscI ACATGT 1 cut(s) 1318
Psp124BI GAGCTC 2 cut(s) 1151, 2469
Psp5II RGGWCCY 1 cut(s) 908
Psp6I CCWGG 5 cut(s) 616, 1552, 2176, 3074, 3184
PspGI CCWGG 5 cut(s) 616, 1552, 2176, 3074, 3184
PspN4I GGNNCC 7 cut(s) 251, 575, 909, 910, 997, 1818, 2761
PspPI GGNCC 6 cut(s) 249, 629, 908, 2282, 2851, 3169
PspPPI RGGWCCY 1 cut(s) 908
PstI CTGCAG 1 cut(s) 942
PstNI CAGNNNCTG 1 cut(s) 744
PsuI RGATCY 8 cut(s) 1214, 1432, 1699, 1714, 2340, 2759, 2976, 3204
PvuII CAGCTG 1 cut(s) 2084
RruI TCGCGA 1 cut(s) 1356
RsaI GTAC 7 cut(s) 866, 1006, 1805, 1939, 2168, 2258, 2888
RsaNI GTAC 7 cut(s) 865, 1005, 1804, 1938, 2167, 2257, 2887
RseI CAYNNNNRTG 2 cut(s) 1407, 2190
SacI GAGCTC 2 cut(s) 1151, 2469
SatI GCNGC 7 cut(s) 14, 32, 1049, 1893, 2523, 2946, 3147
Sau96I GGNCC 6 cut(s) 249, 629, 908, 2282, 2851, 3169
ScaI AGTACT 2 cut(s) 866, 1939
SchI GAGTC 6 cut(s) 17, 268, 454, 1370, 1858, 2554
ScrFI CCNGG 7 cut(s) 476, 477, 618, 1554, 2178, 3076, 3186
SduI GDGCHC 3 cut(s) 1151, 2469, 3103
SexAI ACCWGGT 1 cut(s) 3184
SfaNI GCATC 6 cut(s) 96, 412, 434, 1144, 1191, 2635
SfcI CTRYAG 1 cut(s) 938
SinI GGWCC 6 cut(s) 249, 629, 908, 2282, 2851, 3169
SmaI CCCGGG 1 cut(s) 477
SmiMI CAYNNNNRTG 2 cut(s) 1407, 2190
SmlI CTYRAG 3 cut(s) 315, 1523, 2344
SmoI CTYRAG 3 cut(s) 315, 1523, 2344
SpeI ACTAGT 1 cut(s) 290
SphI GCATGC 2 cut(s) 791, 1232
SsiI CCGC 3 cut(s) 728, 1048, 2854
SstI GAGCTC 2 cut(s) 1151, 2469
StyD4I CCNGG 7 cut(s) 474, 475, 616, 1552, 2176, 3074, 3184
StyI CCWWGG 4 cut(s) 625, 1807, 2387, 2910
TaiI ACGT 4 cut(s) 135, 1775, 2290, 2844
TaqI TCGA 9 cut(s) 46, 352, 771, 1494, 1672, 1781, 1836, 1926, 2643
TaqII GACCGA 1 cut(s) 837
TatI WGTACW 2 cut(s) 864, 1937
TauI GCSGC 1 cut(s) 1051
TfiI GAWTC 8 cut(s) 218, 845, 1669, 2060, 2173, 2234, 2732, 2938
TscAI CASTG 7 cut(s) 338, 489, 1006, 1164, 1731, 1740, 2517
TseFI GTSAC 2 cut(s) 589, 1314
TseI GCWGC 6 cut(s) 13, 31, 1892, 2522, 2945, 3146
Tsp45I GTSAC 2 cut(s) 589, 1314
TspGWI ACGGA 4 cut(s) 1061, 2227, 2281, 3205
TspMI CCCGGG 1 cut(s) 475
TspRI CASTG 7 cut(s) 338, 489, 1006, 1164, 1731, 1740, 2517
Van91I CCANNNNNTGG 1 cut(s) 783
VpaK11BI GGWCC 6 cut(s) 249, 629, 908, 2282, 2851, 3169
XagI CCTNNNNNAGG 1 cut(s) 2984
XapI RAATTY 8 cut(s) 144, 977, 1331, 1533, 1729, 2046, 2552, 2811
XbaI TCTAGA 1 cut(s) 2455
XceI RCATGY 3 cut(s) 791, 1232, 1322
XcmI CCANNNNNNNNNTGG 2 cut(s) 2385, 2417
XmaI CCCGGG 1 cut(s) 475
XmnI GAANNNNTTC 1 cut(s) 1032
ZrmI AGTACT 2 cut(s) 866, 1939
Zsp2I ATGCAT 2 cut(s) 427, 1206
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.