MD02G1064400.v1.1
ERF Family

Probably involved in the RNA silencing pathway and required for the generation of small interfering RNAs (siRNAs)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0000505
Physical Location & Seq
Forward (+)
5198405 .. 5205734
7330 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1064400.v1.1.491

Sequence Viewer

Length: 3330 bp
ATGTATCATCCGCCTAATTACGGTCCCAGCAACTCCAAACGCAGTTACACAGAGATTGAGGGTGTTGATTTCGATGACCCCGTCTTCTCAAATCCCGACCTTTTCCCTCTCAGCTACTCTCACCGCCCCGCTACCGCCGCCATTGGCATTTCCCCACCACACCCAGCAACCGCGGCCACTCCCCGAGACGATTGCCCACAACCGCAACTGCACGTGCCTCTGCCTCCGCGGGTGCAGCAAAAACTGAACAGTATATGTGGGGAGAAACAGCAGCCGCTGCCGGACGCCGAGTTGAGAAGGAAGCTGGGATTGCTGGGGGAGGAGAAGGCACTGGAACTCCTTGGTGACATTAACAACATCAGCACCCTCAGTGGATGCATTTGGTTCTTGCTCCACCATGAAAAGTACCAATGTACCCCTCATCCTCCTTCTCCTTCCCCTTCCCCTTCCAAGTCTGGCCCTGCTGCTTCTCCGCTTCTTCACAGCCCGTCTCCTGCTTCTCAAATCACTCCTGGTCGTCAAGGGAGTACTAGTGGAGGACAGATGCAATTGCATACCTATTTCGCTGCAGCTCCTTCAAAATCTGTACGCGCATCTCTTTTTCAAGGCCCATCATCTGCTCCTATCACTCCTGTTCGTCAAGAGCAACCTGCAGATCGTGGAGGAAGAGAGAGGCCATTGTATTCCTCCTATCCCTCGGATTCTCCTTCGAAAGCTGCCCGTACTTCTCTCTTTCAAGGCCCGTCTTCTGCTTCTACTTCTAAGTCTTCTCTCACTCCGGTTCGACTCTTTCAAGACCAACCAAGACACGATGGGCAGGCACAATTAGAAGCTCTAGGCGAACTTGAATTCAGGAGACAGTTTCTGATATTAAATTACGCCGGAGGAATGAAGTTAGAAGATGTTCTAGCACCAGAGACTATTAGAAGTTGGAAAGATATGCCGATGCAGCAGTTTGAGAACACAATTTGGGAAACTTTGGGTAGGAAATATGCTCGCAAGGAAAACCGACAATGCACTTTGGATTGGGAGTCTGGAAAATCATACGTCTACCATTGTGAAGTCGCTGTGGATGGGAGCTACAATTTTAAGGGCCCCTATCTACACACCGCAAAAAGAACTCTCCTACAGAAAGTTTTAGGAGACGACAGCATTTTAGTGGTGAAATTTGCCGATGTGAAGGATACCAGGAAGCAGAGGGACCTTTATGCTGACTATAGCAAGGTCGCTAGAGAAGGGATTCTTGTTGGTTTGCGCCGATATTGTTTCTTTGTGTTCAAAGATGGTGGCAATAAAGAAAAGAAAAAAAGCCCGACTTCATCACCTGTGAAGTGTTACTTTATCCGTGTGGACTCTAGTGCCGCGATTGACAGGGGAGAAGATTATAAAATATCTAATAAAACGATTCATGAAGCAAGATGCATTTTCATGCATCCACATACTGTGTCCACCGTGAGCAACTACATGATTAGGTTTTCACTCATTTTGTCTAAGACAGAGAGCTTAGAAGTAAAAGATTGGTCTCTTGTGAGGGTTGATGACATTGATGATGAGTATTGTCTGGATGCATACGATAATCGTATCTATAGAGACGGCAAGCCTCTTATACATTCAGATGGAACTGGATTCATATCTGAGGATTTGGCGTCACTTTGTCCGAAAGAATTACTGAAAAAAGAGTTCAGCAATGAATACTTTGAGCCTTTGCTCATGCAGATCCGACTTTTCTACAAGGGCCGTGCTGTGAAGGGAACCTTTCTGGTCAATAAAAAACTACCACAAAAAACGATCCAGATTCGACCATCAATGATTAAAGTTGAGACAGATCGACAGATGCTGGATGGTCAAACAGTGAATTCGTTGGAGATAGTTGGAGTAAGTACAAAGCCTAGGAATACAAAATTTTCGAGGAATTTAATAGCCCTTCTATGCCATGGAGGGGTGCCGAAAGATTATTTCATGGAGTTACTGATGAAAGATCTGGAAAATACTCATGGTGTTTTCTGCAATAGGCGTGCAGCAGCCAAACTTGCCTTTAGCTACGGCGGGATGGATGATGACTACAACACGTTTAAGATGATCTCTTCTGATATTCCTCTAGAAGAATCATATTTGCAACATCGTTTGTCATTCCTGAAGAAAGAGGAAAATATCAGTCTTAAAAGAGGGAAAATTTCTAGTCCTCAGAGTTACATGTTGATGGGGACTACTGATCCAACAGGGATTCTGGAAAAGGATCAAGTTTGCGTTCTTCTTGACAGCGGGCCAATTCAAGGAGAGGTGCTAGTGTACCGGCATCCAGGTTTACATTTTGGTGATGTTCATGTTTTGAAGGCCACCTACGTGGATGAGTTGAAAGAATTTGTTGGAAATAGTGGCTTTGCTATATTCTTCTCTCGCAAAGGTTCGCGTTCAGTAGCTGATGAAATGGGTGGTGGAGATTTTGATGGCGATCTATATTGGGTCTCAAGAAACCCTCGGCTTTTAGAATGTTTCAAACAAGGTGAACCTTGGATCGATACTTCATCAACGCCCAAAGCTGCCACCACCTTACCACCAAGTGCGCTTTTGCCCGATCATATAGAGGATGCGCTCTTTAAACAATTCTTGACAACTAGGTTTGAACCGAGTTTTGCGATGGGTATGGCTTCTGACTACTGGTTGGCTTATATGGATGAATTTTTGACCCTGGGAGATGGTCATGAGAAAACCCTTACGAAGGCAAAAATGCTGCGGTTGATTGATTTATACTACGATGCTTTGGATGCTCCAAAAAAATGTCGAAAGGTCGAAATCCCCAATGATTTGAAACCGCGGTCATTCCCTCATTACATGGATAAGCCCAACTCCTACAAATCAACTTCCATTTTGGGATCAATTTACGACGCAGTGGAGGAATATCAACCGGAAGACACTTCTGACAAAGTAGTGGAAAAACTTCCGTGTTTTGATGTTGAAGAACTCCCCGAGCCGTGCTTGAAGAAGTGGTACAAACTCTACGAGCAGTACAGGAGTGAAATGAACCGTGTCTTGCAATACGATGACAAAGAGGGCAAGAATCAAGCTGCTGATAAGATCATAAGACGGTATAAAGAGATTCTGTATGGCTGTGAGGACTTCGAGAAGAGCACGAGGCCCTTGGATGAAATATTCAACGAGGCACTCGCCATATATCGTTTCGCATATGACTACGCTTGGAAAGTAAACGATATAGGGAAATGTGGGCTTGCATGGAGAGTTGCAGGTTCAGCTCTCTGTAAGTATTACAAGACCAAACACGAGGACCGCACGTTTGAAGTCTCGTTCTCTGTTCTCAAGGATATCCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RdRP

Protein Analysis

1110

Amino Acids

125.67

Weight (kDa)

7.11

Isoelectric Point (pI)

46.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RdRP_helical PF26252 275 - 342 5.8e-06 RNA dependent RNA polymerase, helical domain
RdRP PF05183 362 - 964 4.5e-104 RNA dependent RNA polymerase
RdRP_head PF26253 1013 - 1095 1.1e-11 RdRP, head domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000505)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G19910 AT2G19910 AT2G19920 AT2G19920 AT2G19920 AT2G19920 AT2G19930 AT2G19930 AT2G19930
fragaria_vesca FvH4_1g05980 FvH4_1g05990 FvH4_1g05990 FvH4_1g09718
malus_domestica MD02G1064400.v1.1 MD15G1195300.v1.1 MD15G1195400.v1.1
prunus_persica Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1
pyrus_communis pycom02g05070 pycom15g17460
rosa_chinensis RchiOBHm_Chr2g0091671 RchiOBHm_Chr2g0091681 RchiOBHm_Chr2g0091691 RchiOBHm_Chr2g0091701 RchiOBHm_Chr2g0091711 RchiOBHm_Chr2g0091721 RchiOBHm_Chr2g0091731 RchiOBHm_Chr5g0014401
rosa_laevigata RLG00000013772 RLG00000016242 RLG00000016244 RLG00000016245 RLG00000016246 RLG00000032105
rosa_multiflora Rmu_co8374041.1_g000001 Rmu_co8478121.1_g000001 Rmu_sc0000897.1_g000005 Rmu_sc0000897.1_g000009
rosa_roxburghii Rroxscaffold_1G00062180 Rroxscaffold_1G00062190 Rroxscaffold_1G00068800 Rroxscaffold_2G00149790 Rroxscaffold_2G00149800 Rroxscaffold_2G00149810
rosa_rugosa Rorug02G0019600 Rorug02G0019700 Rorug02G0019800 Rorug02G0019900 Rorug02G0019900 Rorug02G0020000 Rorug02G0020000 Rorug02G0020100 Rorug02G0020100 Rorug02G0020200 Rorug05G0017800
rosa_samantha Rh2AG064700 Rh2AG064800 Rh2AG064900 Rh2BG063800 Rh2BG063900 Rh2BG064000 Rh2BG064100 Rh2CG065500 Rh2CG065600 Rh2CG065700 Rh2DG063900 Rh2DG064000 Rh2DG064100 Rh2DG064200 Rh2DG381700 Rh5AG056700 Rh5AG056800 Rh5AG056900 Rh5AG112600 Rh5BG109300 Rh5CG063800 Rh5CG121100 Rh5DG053100 Rh5DG108300 Rh6CG100100
rosa_wichuraiana Rw2G005560 Rw2G005580 Rw2G005590 Rw5G005370 Rw5G009770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1386
Acc36I ACCTGC 2 cut(s) 658, 3233
AccB1I GGYRCC 1 cut(s) 1942
AccI GTMKAC 1 cut(s) 1050
AccII CGCG 6 cut(s) 173, 229, 591, 1364, 2410, 2815
AclWI GGATC 6 cut(s) 1711, 1783, 2207, 2244, 2522, 2881
AcoI YGGCCR 1 cut(s) 174
AcsI RAATTY 8 cut(s) 848, 1166, 1855, 1901, 1912, 2172, 2360, 2678
AcuI CTGAAG 1 cut(s) 2156
AcvI CACGTG 1 cut(s) 214
AcyI GRCGYC 2 cut(s) 285, 1646
AdeI CACNNNGTG 1 cut(s) 2561
AfaI GTAC 9 cut(s) 407, 415, 529, 588, 724, 1882, 2291, 2990, 3008
AfiI CCNNNNNNNGG 4 cut(s) 20, 1939, 2273, 2719
AflIII ACRYGT 2 cut(s) 2067, 2193
AhlI ACTAGT 1 cut(s) 530
AjnI CCWGG 4 cut(s) 511, 1187, 2299, 2688
AleI CACNNNNGTG 1 cut(s) 2342
Alw21I GWGCWC 1 cut(s) 3131
AlwI GGATC 6 cut(s) 1711, 1783, 2207, 2244, 2522, 2881
AlwNI CAGNNNCTG 4 cut(s) 277, 865, 1837, 2420
Ama87I CYCGRG 2 cut(s) 183, 2966
ApaI GGGCCC 1 cut(s) 1097
ApoI RAATTY 8 cut(s) 848, 1166, 1855, 1901, 1912, 2172, 2360, 2678
ArsI GACNNNNNNTTYG 2 cut(s) 3275, 3307
Asp700I GAANNNNTTC 3 cut(s) 903, 1239, 2937
AspA2I CCTAGG 1 cut(s) 1889
AspLEI GCGC 4 cut(s) 593, 1257, 2566, 2593
AsuHPI GGTGA 6 cut(s) 113, 356, 1174, 1314, 2327, 2516
AsuII TTCGAA 1 cut(s) 710
AvaI CYCGRG 2 cut(s) 183, 2966
AvaII GGWCC 3 cut(s) 23, 1201, 3283
AvrII CCTAGG 1 cut(s) 1889
BaeGI GKGCMC 1 cut(s) 1097
BaeI ACNNNNGTAYC 2 cut(s) 397, 430
BanI GGYRCC 1 cut(s) 1942
BanII GRGCYC 1 cut(s) 1097
BarI GAAGNNNNNNTAC 4 cut(s) 2324, 2356, 2972, 3004
BauI CACGAG 2 cut(s) 3130, 3278
BbrPI CACGTG 1 cut(s) 214
BbsI GAAGAC 4 cut(s) 76, 738, 759, 2916
Bbv12I GWGCWC 1 cut(s) 3131
BceAI ACGGC 4 cut(s) 1609, 1722, 2059, 2956
BcgI CGANNNNNNTGC 2 cut(s) 174, 208
BciT130I CCWGG 4 cut(s) 513, 1189, 2301, 2690
BciVI GTATCC 1 cut(s) 1177
BcuI ACTAGT 1 cut(s) 530
BfmI CTRYAG 5 cut(s) 567, 651, 1127, 1216, 1585
BfuAI ACCTGC 2 cut(s) 658, 3233
BfuI GTATCC 1 cut(s) 1177
BglII AGATCT 1 cut(s) 1978
BlnI CCTAGG 1 cut(s) 1889
BmcAI AGTACT 1 cut(s) 529
Bme1390I CCNGG 4 cut(s) 513, 1189, 2301, 2690
Bme18I GGWCC 3 cut(s) 23, 1201, 3283
BmeT110I CYCGRG 2 cut(s) 183, 2966
BmiI GGNNCC 6 cut(s) 25, 1095, 1096, 1202, 1753, 1944
BmrFI CCNGG 4 cut(s) 513, 1189, 2301, 2690
BpiI GAAGAC 4 cut(s) 76, 738, 759, 2916
Bpu14I TTCGAA 1 cut(s) 710
BpuEI CTTGAG 2 cut(s) 2452, 3299
Bsa29I ATCGAT 1 cut(s) 2517
BsaAI YACGTR 2 cut(s) 214, 2344
BsaBI GATNNNNATC 2 cut(s) 1630, 2451
BsaHI GRCGYC 2 cut(s) 285, 1646
BsaI GGTCTC 2 cut(s) 1527, 2470
BsaWI WCCGGW 2 cut(s) 778, 2905
BsaXI ACNNNNNCTCC 4 cut(s) 321, 351, 517, 547
Bsc4I CCNNNNNNNGG 4 cut(s) 20, 1939, 2273, 2719
Bse118I RCCGGY 1 cut(s) 2292
Bse1I ACTGG 3 cut(s) 336, 1627, 2663
Bse3DI GCAATG 1 cut(s) 1693
Bse8I GATNNNNATC 2 cut(s) 1630, 2451
BseBI CCWGG 4 cut(s) 513, 1189, 2301, 2690
BseCI ATCGAT 1 cut(s) 2517
BseJI GATNNNNATC 2 cut(s) 1630, 2451
BseLI CCNNNNNNNGG 4 cut(s) 20, 1939, 2273, 2719
BseMI GCAATG 1 cut(s) 1693
BseMII CTCAG 4 cut(s) 124, 382, 1626, 2198
BseNI ACTGG 3 cut(s) 336, 1627, 2663
BseRI GAGGAG 1 cut(s) 335
BseSI GKGCMC 1 cut(s) 1097
BseYI CCCAGC 4 cut(s) 26, 163, 304, 313
BsgI GTGCAG 3 cut(s) 194, 254, 2037
Bsh1236I CGCG 6 cut(s) 173, 229, 591, 1364, 2410, 2815
BshNI GGYRCC 1 cut(s) 1942
BshVI ATCGAT 1 cut(s) 2517
BsiHKAI GWGCWC 1 cut(s) 3131
BsiHKCI CYCGRG 2 cut(s) 183, 2966
BsiSI CCGG 5 cut(s) 281, 779, 882, 2293, 2906
BslFI GGGAC 3 cut(s) 9, 1214, 2218
BslI CCNNNNNNNGG 4 cut(s) 20, 1939, 2273, 2719
BsmBI CGTCTC 4 cut(s) 180, 495, 1137, 1584
BsmFI GGGAC 3 cut(s) 9, 1214, 2218
Bso31I GGTCTC 2 cut(s) 1527, 2470
BsoBI CYCGRG 2 cut(s) 183, 2966
Bsp119I TTCGAA 1 cut(s) 710
Bsp120I GGGCCC 1 cut(s) 1093
Bsp1286I GDGCHC 2 cut(s) 1097, 3131
Bsp19I CCATGG 1 cut(s) 1933
BspCNI CTCAG 4 cut(s) 123, 381, 1627, 2197
BspDI ATCGAT 1 cut(s) 2517
BspFNI CGCG 6 cut(s) 173, 229, 591, 1364, 2410, 2815
BspHI TCATGA 2 cut(s) 1408, 2701
BspLI GGNNCC 6 cut(s) 25, 1095, 1096, 1202, 1753, 1944
BspMAI CTGCAG 2 cut(s) 571, 655
BspMI ACCTGC 2 cut(s) 658, 3233
BspPI GGATC 6 cut(s) 1711, 1783, 2207, 2244, 2522, 2881
BspQI GCTCTTC 1 cut(s) 3119
BspT104I TTCGAA 1 cut(s) 710
BspT107I GGYRCC 1 cut(s) 1942
BspTNI GGTCTC 2 cut(s) 1527, 2470
BsrDI GCAATG 1 cut(s) 1693
BsrFI RCCGGY 1 cut(s) 2292
BsrI ACTGG 3 cut(s) 336, 1627, 2663
BssAI RCCGGY 1 cut(s) 2292
BssNI GRCGYC 2 cut(s) 285, 1646
BssSI CACGAG 2 cut(s) 3130, 3278
BssT1I CCWWGG 5 cut(s) 340, 1889, 1933, 2510, 3138
Bst2BI CACGAG 2 cut(s) 3130, 3278
Bst2UI CCWGG 4 cut(s) 513, 1189, 2301, 2690
Bst4CI ACNGT 8 cut(s) 23, 251, 861, 1444, 1453, 1852, 3026, 3087
Bst6I CTCTTC 3 cut(s) 661, 2089, 3119
BstACI GRCGYC 2 cut(s) 285, 1646
BstAPI GCANNNNNTGC 1 cut(s) 277
BstBAI YACGTR 2 cut(s) 214, 2344
BstBI TTCGAA 1 cut(s) 710
BstC8I GCNNGC 6 cut(s) 819, 997, 1598, 2016, 2264, 3228
BstDEI CTNAG 7 cut(s) 110, 368, 762, 1491, 1504, 1635, 2184
BstDSI CCRYGG 4 cut(s) 171, 227, 1933, 2813
BstENI CCTNNNNNAGG 1 cut(s) 2717
BstFNI CGCG 6 cut(s) 173, 229, 591, 1364, 2410, 2815
BstHHI GCGC 4 cut(s) 593, 1257, 2566, 2593
BstMWI GCNNNNNNNGC 9 cut(s) 137, 173, 235, 277, 310, 949, 2030, 2765, 3248
BstNI CCWGG 4 cut(s) 513, 1189, 2301, 2690
BstNSI RCATGY 1 cut(s) 2197
BstSCI CCNGG 4 cut(s) 511, 1187, 2299, 2688
BstSFI CTRYAG 5 cut(s) 567, 651, 1127, 1216, 1585
BstSLI GKGCMC 1 cut(s) 1097
BstUI CGCG 6 cut(s) 173, 229, 591, 1364, 2410, 2815
BstV2I GAAGAC 4 cut(s) 76, 738, 759, 2916
BstX2I RGATCY 2 cut(s) 1716, 1978
BstXI CCANNNNNNTGG 1 cut(s) 2344
BstYI RGATCY 2 cut(s) 1716, 1978
Bsu15I ATCGAT 1 cut(s) 2517
BsuI GTATCC 1 cut(s) 1177
BsuTUI ATCGAT 1 cut(s) 2517
BtgI CCRYGG 4 cut(s) 171, 227, 1933, 2813
BtgZI GCGATG 1 cut(s) 2651
BtsI GCAGTG 1 cut(s) 2895
BtsIMutI CAGTG 4 cut(s) 329, 376, 1857, 2895
BveI ACCTGC 2 cut(s) 658, 3233
Cac8I GCNNGC 6 cut(s) 819, 997, 1598, 2016, 2264, 3228
CaiI CAGNNNCTG 4 cut(s) 277, 865, 1837, 2420
CciI TCATGA 2 cut(s) 1408, 2701
CfoI GCGC 4 cut(s) 593, 1257, 2566, 2593
Cfr10I RCCGGY 1 cut(s) 2292
Cfr42I CCGCGG 3 cut(s) 174, 230, 2816
ClaI ATCGAT 1 cut(s) 2517
CseI GACGC 3 cut(s) 293, 1635, 2894
Csp6I GTAC 9 cut(s) 406, 414, 528, 587, 723, 1881, 2290, 2989, 3007
CspCI CAANNNNNGTGG 2 cut(s) 1267, 1302
CviQI GTAC 9 cut(s) 406, 414, 528, 587, 723, 1881, 2290, 2989, 3007
DdeI CTNAG 7 cut(s) 110, 368, 762, 1491, 1504, 1635, 2184
DraI TTTAAA 1 cut(s) 2599
DraIII CACNNNGTG 1 cut(s) 2561
EaeI YGGCCR 1 cut(s) 174
Eam1104I CTCTTC 3 cut(s) 661, 2089, 3119
EarI CTCTTC 3 cut(s) 661, 2089, 3119
Eco130I CCWWGG 5 cut(s) 340, 1889, 1933, 2510, 3138
Eco24I GRGCYC 1 cut(s) 1097
Eco31I GGTCTC 2 cut(s) 1527, 2470
Eco32I GATATC 1 cut(s) 3322
Eco47I GGWCC 3 cut(s) 23, 1201, 3283
Eco57I CTGAAG 1 cut(s) 2156
Eco72I CACGTG 1 cut(s) 214
Eco88I CYCGRG 2 cut(s) 183, 2966
EcoNI CCTNNNNNAGG 1 cut(s) 2717
EcoO109I RGGNCCY 4 cut(s) 1093, 1094, 1201, 3135
EcoRI GAATTC 2 cut(s) 848, 1855
EcoRII CCWGG 4 cut(s) 511, 1187, 2299, 2688
EcoRV GATATC 1 cut(s) 3322
EcoT14I CCWWGG 5 cut(s) 340, 1889, 1933, 2510, 3138
EcoT22I ATGCAT 4 cut(s) 380, 1424, 1434, 1570
EcoT38I GRGCYC 1 cut(s) 1097
ErhI CCWWGG 5 cut(s) 340, 1889, 1933, 2510, 3138
Esp3I CGTCTC 4 cut(s) 180, 495, 1137, 1584
FalI AAGNNNNNCTT 8 cut(s) 1227, 1259, 1300, 1332, 1322, 1354, 1739, 1771
FaqI GGGAC 3 cut(s) 9, 1214, 2218
FauI CCCGC 4 cut(s) 136, 222, 2039, 2255
FauNDI CATATG 1 cut(s) 3184
FblI GTMKAC 1 cut(s) 1050
FriOI GRGCYC 1 cut(s) 1097
GlaI GCGC 4 cut(s) 592, 1256, 2565, 2592
GsaI CCCAGC 4 cut(s) 30, 167, 308, 317
HapII CCGG 5 cut(s) 281, 779, 882, 2293, 2906
HgaI GACGC 3 cut(s) 293, 1635, 2894
HhaI GCGC 4 cut(s) 593, 1257, 2566, 2593
Hin1I GRCGYC 2 cut(s) 285, 1646
Hin6I GCGC 4 cut(s) 591, 1255, 2564, 2591
HinP1I GCGC 4 cut(s) 591, 1255, 2564, 2591
HpaII CCGG 5 cut(s) 281, 779, 882, 2293, 2906
HphI GGTGA 6 cut(s) 113, 356, 1174, 1314, 2327, 2516
Hpy166II GTNNAC 7 cut(s) 1051, 1351, 1449, 2290, 2306, 2507, 3205
Hpy8I GTNNAC 7 cut(s) 1051, 1351, 1449, 2290, 2306, 2507, 3205
Hpy99I CGWCG 1 cut(s) 2888
HpyCH4III ACNGT 8 cut(s) 23, 251, 861, 1444, 1453, 1852, 3026, 3087
HpyCH4IV ACGT 5 cut(s) 213, 1047, 2069, 2343, 3290
HpyF10VI GCNNNNNNNGC 9 cut(s) 137, 173, 235, 277, 310, 949, 2030, 2765, 3248
HpyF3I CTNAG 7 cut(s) 110, 368, 762, 1491, 1504, 1635, 2184
HpySE526I ACGT 5 cut(s) 213, 1047, 2069, 2343, 3290
Hsp92I GRCGYC 2 cut(s) 285, 1646
HspAI GCGC 4 cut(s) 591, 1255, 2564, 2591
KspI CCGCGG 3 cut(s) 174, 230, 2816
LguI GCTCTTC 1 cut(s) 3119
LmnI GCTCC 5 cut(s) 396, 577, 625, 1077, 2773
MaeII ACGT 5 cut(s) 213, 1047, 2069, 2343, 3290
MaeIII GTNAC 6 cut(s) 44, 344, 1334, 1647, 1965, 2189
MfeI CAATTG 1 cut(s) 548
MflI RGATCY 2 cut(s) 1716, 1978
MhlI GDGCHC 2 cut(s) 1097, 3131
MlyI GAGTC 3 cut(s) 780, 1040, 1346
MmeI TCCRAC 6 cut(s) 911, 1744, 1842, 1852, 2240, 2347
Mph1103I ATGCAT 4 cut(s) 380, 1424, 1434, 1570
MroXI GAANNNNTTC 3 cut(s) 903, 1239, 2937
MseI TTAA 8 cut(s) 351, 872, 1089, 1812, 1916, 2073, 2160, 2598
MslI CAYNNNNRTG 6 cut(s) 1157, 1427, 1614, 2198, 2313, 2342
MspA1I CMGCKG 5 cut(s) 173, 229, 277, 2262, 2815
MspI CCGG 5 cut(s) 281, 779, 882, 2293, 2906
MspR9I CCNGG 4 cut(s) 513, 1189, 2301, 2690
MunI CAATTG 1 cut(s) 548
MvaI CCWGG 4 cut(s) 513, 1189, 2301, 2690
MvnI CGCG 6 cut(s) 173, 229, 591, 1364, 2410, 2815
MwoI GCNNNNNNNGC 9 cut(s) 137, 173, 235, 277, 310, 949, 2030, 2765, 3248
NcoI CCATGG 1 cut(s) 1933
NdeI CATATG 1 cut(s) 3184
NlaIV GGNNCC 6 cut(s) 25, 1095, 1096, 1202, 1753, 1944
NmeAIII GCCGAG 2 cut(s) 313, 2458
NmuCI GTSAC 2 cut(s) 344, 1647
NsiI ATGCAT 4 cut(s) 380, 1424, 1434, 1570
NspI RCATGY 1 cut(s) 2197
NspV TTCGAA 1 cut(s) 710
OliI CACNNNNGTG 1 cut(s) 2342
PagI TCATGA 2 cut(s) 1408, 2701
PasI CCCWGGG 1 cut(s) 2689
PciI ACATGT 1 cut(s) 2193
PciSI GCTCTTC 1 cut(s) 3119
PcsI WCGNNNNNNNCGW 1 cut(s) 78
PdmI GAANNNNTTC 3 cut(s) 903, 1239, 2937
PfeI GAWTC 9 cut(s) 701, 1240, 1405, 1626, 1795, 2105, 2224, 3058, 3097
PflFI GACNNNGTC 1 cut(s) 80
PleI GAGTC 3 cut(s) 780, 1039, 1346
PmaCI CACGTG 1 cut(s) 214
PmlI CACGTG 1 cut(s) 214
PpsI GAGTC 3 cut(s) 780, 1039, 1346
Ppu21I YACGTR 2 cut(s) 214, 2344
PpuMI RGGWCCY 1 cut(s) 1201
PscI ACATGT 1 cut(s) 2193
PsiI TTATAA 1 cut(s) 1386
Psp5II RGGWCCY 1 cut(s) 1201
Psp6I CCWGG 4 cut(s) 511, 1187, 2299, 2688
PspCI CACGTG 1 cut(s) 214
PspFI CCCAGC 4 cut(s) 26, 163, 304, 313
PspGI CCWGG 4 cut(s) 511, 1187, 2299, 2688
PspN4I GGNNCC 6 cut(s) 25, 1095, 1096, 1202, 1753, 1944
PspOMI GGGCCC 1 cut(s) 1093
PspPPI RGGWCCY 1 cut(s) 1201
PstI CTGCAG 2 cut(s) 571, 655
PstNI CAGNNNCTG 4 cut(s) 277, 865, 1837, 2420
PsuI RGATCY 2 cut(s) 1716, 1978
PsyI GACNNNGTC 1 cut(s) 80
RsaI GTAC 9 cut(s) 407, 415, 529, 588, 724, 1882, 2291, 2990, 3008
RsaNI GTAC 9 cut(s) 406, 414, 528, 587, 723, 1881, 2290, 2989, 3007
RseI CAYNNNNRTG 6 cut(s) 1157, 1427, 1614, 2198, 2313, 2342
SacII CCGCGG 3 cut(s) 174, 230, 2816
SapI GCTCTTC 1 cut(s) 3119
SaqAI TTAA 8 cut(s) 351, 872, 1089, 1812, 1916, 2073, 2160, 2598
ScaI AGTACT 1 cut(s) 529
SchI GAGTC 3 cut(s) 780, 1040, 1346
ScrFI CCNGG 4 cut(s) 513, 1189, 2301, 2690
SduI GDGCHC 2 cut(s) 1097, 3131
SfcI CTRYAG 5 cut(s) 567, 651, 1127, 1216, 1585
Sfr303I CCGCGG 3 cut(s) 174, 230, 2816
SfuI TTCGAA 1 cut(s) 710
SgrBI CCGCGG 3 cut(s) 174, 230, 2816
SinI GGWCC 3 cut(s) 23, 1201, 3283
SmiMI CAYNNNNRTG 6 cut(s) 1157, 1427, 1614, 2198, 2313, 2342
SmlI CTYRAG 2 cut(s) 2467, 3314
SmoI CTYRAG 2 cut(s) 2467, 3314
SpeI ACTAGT 1 cut(s) 530
SspI AATATT 1 cut(s) 3150
StyD4I CCNGG 4 cut(s) 511, 1187, 2299, 2688
StyI CCWWGG 5 cut(s) 340, 1889, 1933, 2510, 3138
TaaI ACNGT 8 cut(s) 23, 251, 861, 1444, 1453, 1852, 3026, 3087
TaiI ACGT 5 cut(s) 216, 1050, 2072, 2346, 3293
TatI WGTACW 3 cut(s) 527, 1880, 3006
TauI GCSGC 4 cut(s) 140, 176, 277, 1364
TfiI GAWTC 9 cut(s) 701, 1240, 1405, 1626, 1795, 2105, 2224, 3058, 3097
Tru1I TTAA 8 cut(s) 351, 872, 1089, 1812, 1916, 2073, 2160, 2598
Tru9I TTAA 8 cut(s) 351, 872, 1089, 1812, 1916, 2073, 2160, 2598
TscAI CASTG 4 cut(s) 336, 376, 1857, 2895
TseFI GTSAC 2 cut(s) 344, 1647
Tsp45I GTSAC 2 cut(s) 344, 1647
TspGWI ACGGA 2 cut(s) 1334, 2931
TspRI CASTG 4 cut(s) 336, 376, 1857, 2895
Tth111I GACNNNGTC 1 cut(s) 80
VpaK11BI GGWCC 3 cut(s) 23, 1201, 3283
XagI CCTNNNNNAGG 1 cut(s) 2717
XapI RAATTY 8 cut(s) 848, 1166, 1855, 1901, 1912, 2172, 2360, 2678
XbaI TCTAGA 1 cut(s) 2098
XceI RCATGY 1 cut(s) 2197
XmaJI CCTAGG 1 cut(s) 1889
XmiI GTMKAC 1 cut(s) 1050
XmnI GAANNNNTTC 3 cut(s) 903, 1239, 2937
ZrmI AGTACT 1 cut(s) 529
Zsp2I ATGCAT 4 cut(s) 380, 1424, 1434, 1570
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.