Rroxscaffold_1G00062180
ERF Family

Probably involved in the RNA silencing pathway and required for the generation of small interfering RNAs (siRNAs)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000505
Physical Location & Seq
Forward (+)
84211226 .. 84219392
8167 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00062180.1

Sequence Viewer

Length: 2535 bp
ATGTTCGGCGTGCAGCTACTCGACTCCGTGCAGCAATCGCTGGATCGAATATGTCACCTTAAGAAGCAACCGCAGCCGGACGACGGCATCAGAAGAAAGCTGGCATTGCTGAGCGAGCAAGACGCCCTCTACGTGCTGGACCAAATTTACAACAGCAAAAATGAGATCAGAACTCTCAATGGATTCATTGCTTTCATGATCAGGAAACTAGAGAATGAATCTTCTTCGTCTCCTTCTTCTAGAACAGTGTGGACCCCTCAGAGTCCCTCTGCTTCTTCTTCTTCTGTTACTAGTCCTGCACAGCTTTATCACAGCCCTGATCATTCTCAAGGTTTGGATTTTTTACTTTCTTTGTCAAGTTGCAAGCAATTTCCGTCTCAATTTGAGTTTGAGGGTGCGGTGGTGTTTGCAGTTGTAGACCAGTCTGAAGAGAGTGGACTCTGTTCCTCTGTTTTTCAGCGGCTATATTTGAGCTCTCAAGGTGAGACAATTCCTTGTGGTTCTCCTGGTGTAGAGCAACCTCAAGACTATAATCGACAGCCACTCTATTCTTCAGCTTCTCCTTCTGAAGCTTTCCAGACTTCTTATTATCAGAGCCATTCTTCTCCTACTAGTACTGATGCACCATTTTATTCCAGTTCTCAGCCTCAGGGTGAGATTCCCAACCCATCCCCGATGACAGTGTGCAATGGCCAATCACCTTATTCCTCCCCTTCTCCTTATCCAATCCTGTCTGCTCCTTCTAATACTCATAGACAACTCTATGAGAGCAATGATGGTGTCCATATTTCTCCTTTTCAAACCGTATCTGCCTCTCCTGTTACTCCTGTTCAACTTTATAAGAGCTTTCAAGGTGAGACTGTCACTCACTCCCCAGTTCTTTACCATCCTGGTGATCATGGACCAGCACACTTGGAAGCTCTGGGAGAACTCGAGTTCAGAAGACAATTTCTAATATTAAGTTATGCTGGAGGAACGAAGTTAAAAGATGTAATAGAAGTGGAGGCTATTAGGGGCTGGAAAAATTTGCCAATGGTAAACTTCGAGACCAAAGTTTGGCATGCTTTGGGTGAAAACAATGTTGGCAAGGCTGACCGACGATTGACATCAGATAGCAAGTTAATGCGAGAAGGCATTCTTGTTGGTTTGCGGCGATATGGTCACTTTGTGTACAGTGTTGGTGGTAACAAAGGAAAGAATAAGTTGAAAGACCCAACTTCATCATCTGTGAAATGTTACTTTGTTCGTATGAGCTCTGATGCAGCGATAGACATGGGTAAATTCTTTAAGTTAGCTGGCAAAACGATGCATGAAGGAAGATCTCTTTTGACGCACGTACACACTGGGCCTAGTTTGGCTAATTACATGGCTAGGTTATCTCTCATTTTGTCCAAGACAGTTACTTTAGAAGTGAATTGGTCACTTGTCAAGGTTGAGTTCATCAATGAAGAATACTGTCTCGATGAGTCTGATAATCCCATCTATGTAGATGGAAAAGCTCGTATACATACTGATGGCACGGGATTTATATCTGAGGATCTGGCTTTACTTTGCCCTGAGATAGCACACAAAGGAGAGCTTATCAATGATGAACACATCGAGGGACTTGATGATCCTGATGATGAGCTTGAGGACAACATTCTGGAATGGAAACAGCCAGGCACTAGAACTCAGGAACTGGAATGCAATTCTTCTAATTTATCTGAAGCAATACCATCTAAAAGGTTTGTGATTGATGGAATGCTTCGATTTCTATTTCAAAATTTGCAGCTTCCACCAAACACATTGCAGATTCGACCATCAATGGTCAAAGTTGTGCCAGATCCAAAATTGTCTGATCCTCAAACAGTGAATTCACTGGAGATAGTAGGAACAAGTAAACTTACTAAGAGAACAACTTTGTCGAGGAACCTGATTGCACTTTTATACCATGGAGGGGTGCCAAAAGAATACTTCATCGAGTTACTTATGAAAGCTTTAGAGGAGACTCGTGATGTTTATAGCAATAAACGTGCAGCATTCAGAGTTGCCCTTAGATATGGCGAGATCGACGACAATAGTAATGTTGCGAAAATGATCTCCTGTGGTATTCCTCTTGAAGAACCATATGTGCAATATCTCCCCCCAGCCCCCGATTCGTTCTCTCTCTTTTCCCTCGGACCCGCCGGTCTTCTTCGACCCGACCCGGCTGGAAAACGCCCCTCCGGCCTCCTCCGACGCCGACCCGCCTCCACCGCCATCGGACCAGGCCGCCCGTGCTCTCTATGGACCTCCCTTGCACCGCCGCTGCCCCCGCACACGGATCGAAGCCACCCCGCCCTCCGCCGACCGACCCGGCCGGAAAACGAGTTTTCCGGCGTTGCACGGGCCGATTCTTCCCACCATGGTGATCTCCTCCTCTCGCCGCCGATCATCCCCATGCCCTCCTTGCTTGACGATTTTGAGTGTGGAGCGAAAGATCAAGGATTGAAGCCAACGGTTCGGTTGAATCGACCAAATCGGACGGCCACGATTAATCCAAACTTCAAGGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RdRP

Protein Analysis

844

Amino Acids

93.4

Weight (kDa)

6.18

Isoelectric Point (pI)

60.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
4HB_RdRP3_N PF26249 6 - 69 9.5e-08 RdRP3/4/5, N-terminal four helical bundle domain
RdRP_helical PF26252 304 - 369 3.9e-07 RNA dependent RNA polymerase, helical domain
RdRP PF05183 367 - 519 1.2e-11 RNA dependent RNA polymerase
RdRP PF05183 592 - 705 4.5e-15 RNA dependent RNA polymerase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000505)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G19910 AT2G19910 AT2G19920 AT2G19920 AT2G19920 AT2G19920 AT2G19930 AT2G19930 AT2G19930
fragaria_vesca FvH4_1g05980 FvH4_1g05990 FvH4_1g05990 FvH4_1g09718
malus_domestica MD02G1064400.v1.1 MD15G1195300.v1.1 MD15G1195400.v1.1
prunus_persica Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1
pyrus_communis pycom02g05070 pycom15g17460
rosa_chinensis RchiOBHm_Chr2g0091671 RchiOBHm_Chr2g0091681 RchiOBHm_Chr2g0091691 RchiOBHm_Chr2g0091701 RchiOBHm_Chr2g0091711 RchiOBHm_Chr2g0091721 RchiOBHm_Chr2g0091731 RchiOBHm_Chr5g0014401
rosa_laevigata RLG00000013772 RLG00000016242 RLG00000016244 RLG00000016245 RLG00000016246 RLG00000032105
rosa_multiflora Rmu_co8374041.1_g000001 Rmu_co8478121.1_g000001 Rmu_sc0000897.1_g000005 Rmu_sc0000897.1_g000009
rosa_roxburghii Rroxscaffold_1G00062180 Rroxscaffold_1G00062190 Rroxscaffold_1G00068800 Rroxscaffold_2G00149790 Rroxscaffold_2G00149800 Rroxscaffold_2G00149810
rosa_rugosa Rorug02G0019600 Rorug02G0019700 Rorug02G0019800 Rorug02G0019900 Rorug02G0019900 Rorug02G0020000 Rorug02G0020000 Rorug02G0020100 Rorug02G0020100 Rorug02G0020200 Rorug05G0017800
rosa_samantha Rh2AG064700 Rh2AG064800 Rh2AG064900 Rh2BG063800 Rh2BG063900 Rh2BG064000 Rh2BG064100 Rh2CG065500 Rh2CG065600 Rh2CG065700 Rh2DG063900 Rh2DG064000 Rh2DG064100 Rh2DG064200 Rh2DG381700 Rh5AG056700 Rh5AG056800 Rh5AG056900 Rh5AG112600 Rh5BG109300 Rh5CG063800 Rh5CG121100 Rh5DG053100 Rh5DG108300 Rh6CG100100
rosa_wichuraiana Rw2G005560 Rw2G005580 Rw2G005590 Rw5G005370 Rw5G009770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 840
AasI GACNNNNNNGTC 1 cut(s) 2166
AccB1I GGYRCC 1 cut(s) 1939
AccB7I CCANNNNNTGG 1 cut(s) 1056
AccI GTMKAC 2 cut(s) 417, 1504
AclWI GGATC 6 cut(s) 51, 1545, 1607, 1817, 1832, 2310
AcoI YGGCCR 3 cut(s) 691, 2336, 2505
AcsI RAATTY 5 cut(s) 144, 1024, 1280, 1762, 1852
AcuI CTGAAG 4 cut(s) 447, 537, 588, 1725
AcyI GRCGYC 2 cut(s) 123, 2218
AdeI CACNNNGTG 1 cut(s) 1168
AfaI GTAC 3 cut(s) 616, 1172, 1338
AfiI CCNNNNNNNGG 4 cut(s) 83, 1056, 1936, 2299
AflII CTTAAG 1 cut(s) 59
AgsI TTSAA 9 cut(s) 800, 833, 851, 1207, 1760, 2099, 2470, 2488, 2527
AhlI ACTAGT 2 cut(s) 290, 611
AjnI CCWGG 4 cut(s) 505, 889, 1657, 2245
AjuI GAANNNNNNNTTGG 2 cut(s) 1065, 1097
AleI CACNNNNGTG 1 cut(s) 2385
Alw21I GWGCWC 3 cut(s) 476, 1256, 2261
Alw26I GTCTC 7 cut(s) 234, 381, 479, 851, 1040, 1463, 1979
AlwI GGATC 6 cut(s) 51, 1545, 1607, 1817, 1832, 2310
AlwNI CAGNNNCTG 1 cut(s) 1678
Ama87I CYCGRG 1 cut(s) 932
AoxI GGCC 7 cut(s) 691, 1346, 2206, 2248, 2336, 2367, 2505
ApeKI GCWGC 7 cut(s) 13, 31, 73, 1262, 1768, 2015, 2287
ApoI RAATTY 5 cut(s) 144, 1024, 1280, 1762, 1852
AseI ATTAAT 1 cut(s) 2514
Asp700I GAANNNNTTC 1 cut(s) 1134
AspS9I GGNCC 8 cut(s) 139, 252, 902, 1346, 2159, 2243, 2268, 2367
AsuC2I CCSGG 2 cut(s) 2186, 2335
AsuHPI GGTGA 8 cut(s) 47, 494, 665, 690, 866, 905, 1082, 2399
AvaI CYCGRG 1 cut(s) 932
AvaII GGWCC 6 cut(s) 139, 252, 902, 2159, 2243, 2268
AxyI CCTNAGG 1 cut(s) 648
BalI TGGCCA 1 cut(s) 693
BanI GGYRCC 1 cut(s) 1939
BanII GRGCYC 2 cut(s) 476, 1256
BauI CACGAG 1 cut(s) 1989
BbsI GAAGAC 2 cut(s) 949, 2162
Bbv12I GWGCWC 3 cut(s) 476, 1256, 2261
BbvI GCAGC 7 cut(s) 25, 43, 85, 1274, 1780, 2027, 2274
BceAI ACGGC 2 cut(s) 100, 2520
BcgI CGANNNNNNTGC 2 cut(s) 2285, 2319
BciT130I CCWGG 4 cut(s) 507, 891, 1659, 2247
BclI TGATCA 3 cut(s) 198, 319, 895
BcnI CCSGG 2 cut(s) 2186, 2335
BcoDI GTCTC 7 cut(s) 234, 381, 479, 851, 1040, 1463, 1979
BcuI ACTAGT 2 cut(s) 290, 611
BfaI CTAG 7 cut(s) 209, 240, 291, 612, 1350, 1371, 1665
BfrI CTTAAG 1 cut(s) 59
BglI GCCNNNNNGGC 1 cut(s) 2205
BglII AGATCT 1 cut(s) 1319
BlpI GCTNAGC 1 cut(s) 110
BmcAI AGTACT 1 cut(s) 616
Bme1390I CCNGG 6 cut(s) 507, 891, 1659, 2186, 2247, 2335
Bme18I GGWCC 6 cut(s) 139, 252, 902, 2159, 2243, 2268
BmeT110I CYCGRG 1 cut(s) 932
BmgT120I GGNCC 8 cut(s) 139, 252, 902, 1346, 2159, 2243, 2268, 2367
BmiI GGNNCC 4 cut(s) 254, 1910, 1941, 2161
BmrFI CCNGG 6 cut(s) 507, 891, 1659, 2186, 2247, 2335
BmrI ACTGGG 2 cut(s) 869, 1353
BmsI GCATC 4 cut(s) 96, 610, 1249, 1296
BmuI ACTGGG 2 cut(s) 869, 1353
BpiI GAAGAC 2 cut(s) 949, 2162
BpmI CTGGAG 2 cut(s) 990, 1880
Bpu1102I GCTNAGC 1 cut(s) 110
BpuEI CTTGAG 4 cut(s) 312, 462, 507, 1649
BpuMI CCSGG 2 cut(s) 2186, 2335
BsaAI YACGTR 2 cut(s) 133, 1336
BsaBI GATNNNNATC 2 cut(s) 1105, 1528
BsaHI GRCGYC 2 cut(s) 123, 2218
BsaI GGTCTC 1 cut(s) 1040
BsaJI CCNNGG 3 cut(s) 1930, 2155, 2383
Bsc4I CCNNNNNNNGG 4 cut(s) 83, 1056, 1936, 2299
Bse118I RCCGGY 1 cut(s) 2165
Bse1I ACTGG 6 cut(s) 421, 636, 875, 1348, 1683, 1863
Bse21I CCTNAGG 1 cut(s) 648
Bse3DI GCAATG 5 cut(s) 104, 186, 694, 778, 1784
Bse8I GATNNNNATC 2 cut(s) 1105, 1528
BseBI CCWGG 4 cut(s) 507, 891, 1659, 2247
BseDI CCNNGG 3 cut(s) 1930, 2155, 2383
BseGI GGATG 3 cut(s) 668, 886, 2412
BseJI GATNNNNATC 2 cut(s) 1105, 1528
BseLI CCNNNNNNNGG 4 cut(s) 83, 1056, 1936, 2299
BseMI GCAATG 5 cut(s) 104, 186, 694, 778, 1784
BseMII CTCAG 7 cut(s) 101, 272, 656, 662, 1524, 1548, 1685
BseNI ACTGG 6 cut(s) 421, 636, 875, 1348, 1683, 1863
BseRI GAGGAG 4 cut(s) 1997, 2201, 2384, 2387
BseX3I CGGCCG 1 cut(s) 2336
BseXI GCAGC 7 cut(s) 25, 43, 85, 1274, 1780, 2027, 2274
BseYI CCCAGC 1 cut(s) 2125
BsgI GTGCAG 4 cut(s) 32, 50, 282, 2034
Bsh1285I CGRYCG 2 cut(s) 2330, 2339
BshFI GGCC 7 cut(s) 693, 1348, 2208, 2250, 2338, 2369, 2507
BshNI GGYRCC 1 cut(s) 1939
BsiEI CGRYCG 2 cut(s) 2330, 2339
BsiHKAI GWGCWC 3 cut(s) 476, 1256, 2261
BsiHKCI CYCGRG 1 cut(s) 932
BsiSI CCGG 7 cut(s) 77, 2166, 2186, 2205, 2335, 2339, 2355
BslFI GGGAC 2 cut(s) 249, 1617
BslI CCNNNNNNNGG 4 cut(s) 83, 1056, 1936, 2299
BsmAI GTCTC 7 cut(s) 234, 381, 479, 851, 1040, 1463, 1979
BsmBI CGTCTC 2 cut(s) 234, 381
BsmFI GGGAC 2 cut(s) 249, 1617
BsmI GAATGC 4 cut(s) 1134, 1688, 1746, 2018
BsnI GGCC 7 cut(s) 693, 1348, 2208, 2250, 2338, 2369, 2507
Bso31I GGTCTC 1 cut(s) 1040
BsoBI CYCGRG 1 cut(s) 932
Bsp1286I GDGCHC 3 cut(s) 476, 1256, 2261
Bsp1407I TGTACA 1 cut(s) 1170
Bsp1720I GCTNAGC 1 cut(s) 110
Bsp19I CCATGG 2 cut(s) 1930, 2383
BspANI GGCC 7 cut(s) 693, 1348, 2208, 2250, 2338, 2369, 2507
BspCNI CTCAG 7 cut(s) 102, 271, 655, 661, 1525, 1549, 1684
BspHI TCATGA 1 cut(s) 195
BspLI GGNNCC 4 cut(s) 254, 1910, 1941, 2161
BspPI GGATC 6 cut(s) 51, 1545, 1607, 1817, 1832, 2310
BspT107I GGYRCC 1 cut(s) 1939
BspTI CTTAAG 1 cut(s) 59
BspTNI GGTCTC 1 cut(s) 1040
BsrDI GCAATG 5 cut(s) 104, 186, 694, 778, 1784
BsrFI RCCGGY 1 cut(s) 2165
BsrGI TGTACA 1 cut(s) 1170
BsrI ACTGG 6 cut(s) 421, 636, 875, 1348, 1683, 1863
BssAI RCCGGY 1 cut(s) 2165
BssECI CCNNGG 3 cut(s) 1930, 2155, 2383
BssNAI GTATAC 1 cut(s) 1505
BssNI GRCGYC 2 cut(s) 123, 2218
BssSI CACGAG 1 cut(s) 1989
BssT1I CCWWGG 2 cut(s) 1930, 2383
Bst1107I GTATAC 1 cut(s) 1505
Bst2BI CACGAG 1 cut(s) 1989
Bst2UI CCWGG 4 cut(s) 507, 891, 1659, 2247
Bst4CI ACNGT 9 cut(s) 247, 682, 805, 862, 1175, 1399, 1457, 1849, 2479
Bst6I CTCTTC 1 cut(s) 423
BstACI GRCGYC 2 cut(s) 123, 2218
BstAFI CTTAAG 1 cut(s) 59
BstAUI TGTACA 1 cut(s) 1170
BstBAI YACGTR 2 cut(s) 133, 1336
BstC8I GCNNGC 6 cut(s) 11, 102, 116, 365, 1062, 1297
BstDEI CTNAG 9 cut(s) 110, 258, 642, 648, 1533, 1557, 1671, 1887, 2033
BstDSI CCRYGG 2 cut(s) 1930, 2383
BstF5I GGATG 3 cut(s) 668, 886, 2412
BstMAI GTCTC 7 cut(s) 234, 381, 479, 851, 1040, 1463, 1979
BstMCI CGRYCG 2 cut(s) 2330, 2339
BstMWI GCNNNNNNNGC 9 cut(s) 37, 73, 106, 115, 2205, 2234, 2256, 2293, 2428
BstNI CCWGG 4 cut(s) 507, 891, 1659, 2247
BstNSI RCATGY 1 cut(s) 1064
BstSCI CCNGG 6 cut(s) 505, 889, 1657, 2184, 2245, 2333
BstV1I GCAGC 7 cut(s) 25, 43, 85, 1274, 1780, 2027, 2274
BstV2I GAAGAC 2 cut(s) 949, 2162
BstX2I RGATCY 3 cut(s) 1319, 1537, 1822
BstYI RGATCY 3 cut(s) 1319, 1537, 1822
BstZ17I GTATAC 1 cut(s) 1505
BstZI CGGCCG 1 cut(s) 2336
Bsu36I CCTNAGG 1 cut(s) 648
BsuRI GGCC 7 cut(s) 693, 1348, 2208, 2250, 2338, 2369, 2507
BtgI CCRYGG 2 cut(s) 1930, 2383
BtsCI GGATG 3 cut(s) 668, 886, 2412
BtsIMutI CAGTG 6 cut(s) 252, 687, 1180, 1341, 1854, 1856
Cac8I GCNNGC 6 cut(s) 11, 102, 116, 365, 1062, 1297
CaiI CAGNNNCTG 1 cut(s) 1678
CciI TCATGA 1 cut(s) 195
Cfr10I RCCGGY 1 cut(s) 2165
Cfr13I GGNCC 8 cut(s) 139, 252, 902, 1346, 2159, 2243, 2268, 2367
CseI GACGC 3 cut(s) 131, 1339, 2226
Csp6I GTAC 3 cut(s) 615, 1171, 1337
CviAII CATG 9 cut(s) 196, 899, 1061, 1273, 1310, 1366, 1931, 2384, 2419
CviQI GTAC 3 cut(s) 615, 1171, 1337
DdeI CTNAG 9 cut(s) 110, 258, 642, 648, 1533, 1557, 1671, 1887, 2033
DraIII CACNNNGTG 1 cut(s) 1168
DrdI GACNNNNNNGTC 1 cut(s) 2166
DseDI GACNNNNNNGTC 1 cut(s) 2166
EaeI YGGCCR 3 cut(s) 691, 2336, 2505
EagI CGGCCG 1 cut(s) 2336
Eam1104I CTCTTC 1 cut(s) 423
EarI CTCTTC 1 cut(s) 423
EciI GGCGGA 1 cut(s) 2312
Ecl136II GAGCTC 2 cut(s) 474, 1254
EclXI CGGCCG 1 cut(s) 2336
Eco130I CCWWGG 2 cut(s) 1930, 2383
Eco24I GRGCYC 2 cut(s) 476, 1256
Eco31I GGTCTC 1 cut(s) 1040
Eco47I GGWCC 6 cut(s) 139, 252, 902, 2159, 2243, 2268
Eco52I CGGCCG 1 cut(s) 2336
Eco53kI GAGCTC 2 cut(s) 474, 1254
Eco57I CTGAAG 4 cut(s) 447, 537, 588, 1725
Eco81I CCTNAGG 1 cut(s) 648
Eco88I CYCGRG 1 cut(s) 932
EcoICRI GAGCTC 2 cut(s) 474, 1254
EcoRI GAATTC 1 cut(s) 1852
EcoRII CCWGG 4 cut(s) 505, 889, 1657, 2245
EcoT14I CCWWGG 2 cut(s) 1930, 2383
EcoT22I ATGCAT 1 cut(s) 1311
EcoT38I GRGCYC 2 cut(s) 476, 1256
ErhI CCWWGG 2 cut(s) 1930, 2383
Esp3I CGTCTC 2 cut(s) 234, 381
FaeI CATG 9 cut(s) 199, 902, 1064, 1276, 1313, 1369, 1934, 2387, 2422
FalI AAGNNNNNCTT 4 cut(s) 1122, 1154, 1563, 1595
FaqI GGGAC 2 cut(s) 249, 1617
FatI CATG 9 cut(s) 195, 898, 1060, 1272, 1309, 1365, 1930, 2383, 2418
FauI CCCGC 4 cut(s) 2170, 2233, 2301, 2323
FauNDI CATATG 1 cut(s) 2107
FbaI TGATCA 3 cut(s) 198, 319, 895
FblI GTMKAC 2 cut(s) 417, 1504
FokI GGATG 3 cut(s) 655, 873, 2399
FriOI GRGCYC 2 cut(s) 476, 1256
FspBI CTAG 7 cut(s) 209, 240, 291, 612, 1350, 1371, 1665
GsaI CCCAGC 1 cut(s) 2129
GsuI CTGGAG 2 cut(s) 990, 1880
HaeIII GGCC 7 cut(s) 693, 1348, 2208, 2250, 2338, 2369, 2507
HapII CCGG 7 cut(s) 77, 2166, 2186, 2205, 2335, 2339, 2355
HgaI GACGC 3 cut(s) 131, 1339, 2226
Hin1I GRCGYC 2 cut(s) 123, 2218
Hin1II CATG 9 cut(s) 199, 902, 1064, 1276, 1313, 1369, 1934, 2387, 2422
HindIII AAGCTT 2 cut(s) 570, 1974
HpaII CCGG 7 cut(s) 77, 2166, 2186, 2205, 2335, 2339, 2355
HphI GGTGA 8 cut(s) 47, 494, 665, 690, 866, 905, 1082, 2399
Hpy166II GTNNAC 8 cut(s) 252, 418, 437, 1039, 1171, 1339, 1505, 1880
Hpy8I GTNNAC 8 cut(s) 252, 418, 437, 1039, 1171, 1339, 1505, 1880
Hpy99I CGWCG 4 cut(s) 86, 1101, 2054, 2220
HpyAV CCTTC 6 cut(s) 243, 573, 723, 750, 1124, 1307
HpyCH4III ACNGT 9 cut(s) 247, 682, 805, 862, 1175, 1399, 1457, 1849, 2479
HpyCH4IV ACGT 3 cut(s) 132, 1335, 2011
HpyF10VI GCNNNNNNNGC 9 cut(s) 37, 73, 106, 115, 2205, 2234, 2256, 2293, 2428
HpyF3I CTNAG 9 cut(s) 110, 258, 642, 648, 1533, 1557, 1671, 1887, 2033
HpySE526I ACGT 3 cut(s) 132, 1335, 2011
Hsp92I GRCGYC 2 cut(s) 123, 2218
Hsp92II CATG 9 cut(s) 199, 902, 1064, 1276, 1313, 1369, 1934, 2387, 2422
Ksp22I TGATCA 3 cut(s) 198, 319, 895
LmnI GCTCC 2 cut(s) 742, 2450
Lsp1109I GCAGC 7 cut(s) 25, 43, 85, 1274, 1780, 2027, 2274
LweI GCATC 4 cut(s) 96, 610, 1249, 1296
MaeI CTAG 7 cut(s) 209, 240, 291, 612, 1350, 1371, 1665
MaeII ACGT 3 cut(s) 132, 1335, 2011
MflI RGATCY 3 cut(s) 1319, 1537, 1822
MhlI GDGCHC 3 cut(s) 476, 1256, 2261
MlsI TGGCCA 1 cut(s) 693
MluNI TGGCCA 1 cut(s) 693
MlyI GAGTC 5 cut(s) 17, 271, 432, 1475, 1981
MmeI TCCRAC 1 cut(s) 2239
Mox20I TGGCCA 1 cut(s) 693
Mph1103I ATGCAT 1 cut(s) 1311
MroXI GAANNNNTTC 1 cut(s) 1134
MscI TGGCCA 1 cut(s) 693
MseI TTAA 6 cut(s) 60, 959, 983, 1121, 1287, 2514
MslI CAYNNNNRTG 4 cut(s) 891, 1512, 2385, 2417
Msp20I TGGCCA 1 cut(s) 693
MspA1I CMGCKG 2 cut(s) 460, 2287
MspCI CTTAAG 1 cut(s) 59
MspI CCGG 7 cut(s) 77, 2166, 2186, 2205, 2335, 2339, 2355
MspR9I CCNGG 6 cut(s) 507, 891, 1659, 2186, 2247, 2335
Mva1269I GAATGC 4 cut(s) 1134, 1688, 1746, 2018
MvaI CCWGG 4 cut(s) 507, 891, 1659, 2247
MwoI GCNNNNNNNGC 9 cut(s) 37, 73, 106, 115, 2205, 2234, 2256, 2293, 2428
NciI CCSGG 2 cut(s) 2186, 2335
NcoI CCATGG 2 cut(s) 1930, 2383
NdeI CATATG 1 cut(s) 2107
NlaIII CATG 9 cut(s) 199, 902, 1064, 1276, 1313, 1369, 1934, 2387, 2422
NlaIV GGNNCC 4 cut(s) 254, 1910, 1941, 2161
NmuCI GTSAC 4 cut(s) 53, 862, 1160, 1419
NsiI ATGCAT 1 cut(s) 1311
NspI RCATGY 1 cut(s) 1064
OliI CACNNNNGTG 1 cut(s) 2385
PaeI GCATGC 1 cut(s) 1064
PaeR7I CTCGAG 1 cut(s) 932
PagI TCATGA 1 cut(s) 195
PcsI WCGNNNNNNNCGW 2 cut(s) 129, 2488
PctI GAATGC 4 cut(s) 1134, 1688, 1746, 2018
PdmI GAANNNNTTC 1 cut(s) 1134
PfeI GAWTC 7 cut(s) 183, 218, 658, 1792, 2135, 2372, 2488
PflMI CCANNNNNTGG 1 cut(s) 1056
PleI GAGTC 5 cut(s) 17, 270, 432, 1474, 1981
PpsI GAGTC 5 cut(s) 17, 270, 432, 1474, 1981
Ppu21I YACGTR 2 cut(s) 133, 1336
PshBI ATTAAT 1 cut(s) 2514
PsiI TTATAA 1 cut(s) 840
Psp124BI GAGCTC 2 cut(s) 476, 1256
Psp6I CCWGG 4 cut(s) 505, 889, 1657, 2245
PspFI CCCAGC 1 cut(s) 2125
PspGI CCWGG 4 cut(s) 505, 889, 1657, 2245
PspN4I GGNNCC 4 cut(s) 254, 1910, 1941, 2161
PspPI GGNCC 8 cut(s) 139, 252, 902, 1346, 2159, 2243, 2268, 2367
PspXI VCTCGAGB 1 cut(s) 932
PstNI CAGNNNCTG 1 cut(s) 1678
PsuI RGATCY 3 cut(s) 1319, 1537, 1822
RsaI GTAC 3 cut(s) 616, 1172, 1338
RsaNI GTAC 3 cut(s) 615, 1171, 1337
RseI CAYNNNNRTG 4 cut(s) 891, 1512, 2385, 2417
SacI GAGCTC 2 cut(s) 476, 1256
SaqAI TTAA 6 cut(s) 60, 959, 983, 1121, 1287, 2514
Sau96I GGNCC 8 cut(s) 139, 252, 902, 1346, 2159, 2243, 2268, 2367
ScaI AGTACT 1 cut(s) 616
SchI GAGTC 5 cut(s) 17, 271, 432, 1475, 1981
ScrFI CCNGG 6 cut(s) 507, 891, 1659, 2186, 2247, 2335
SduI GDGCHC 3 cut(s) 476, 1256, 2261
SfaNI GCATC 4 cut(s) 96, 610, 1249, 1296
Sfr274I CTCGAG 1 cut(s) 932
SinI GGWCC 6 cut(s) 139, 252, 902, 2159, 2243, 2268
SlaI CTCGAG 1 cut(s) 932
SmiMI CAYNNNNRTG 4 cut(s) 891, 1512, 2385, 2417
SmlI CTYRAG 6 cut(s) 59, 327, 477, 522, 932, 1628
SmoI CTYRAG 6 cut(s) 59, 327, 477, 522, 932, 1628
SpeI ACTAGT 2 cut(s) 290, 611
SphI GCATGC 1 cut(s) 1064
SspI AATATT 1 cut(s) 957
SspMI CTAG 7 cut(s) 209, 240, 291, 612, 1350, 1371, 1665
SstI GAGCTC 2 cut(s) 476, 1256
StyD4I CCNGG 6 cut(s) 505, 889, 1657, 2184, 2245, 2333
StyI CCWWGG 2 cut(s) 1930, 2383
TaaI ACNGT 9 cut(s) 247, 682, 805, 862, 1175, 1399, 1457, 1849, 2479
TaiI ACGT 3 cut(s) 135, 1338, 2014
TaqII GACCGA 2 cut(s) 1110, 2344
TatI WGTACW 2 cut(s) 614, 1170
TauI GCSGC 5 cut(s) 463, 1153, 2253, 2287, 2407
TfiI GAWTC 7 cut(s) 183, 218, 658, 1792, 2135, 2372, 2488
Tru1I TTAA 6 cut(s) 60, 959, 983, 1121, 1287, 2514
Tru9I TTAA 6 cut(s) 60, 959, 983, 1121, 1287, 2514
TscAI CASTG 6 cut(s) 252, 687, 1180, 1348, 1854, 1863
TseFI GTSAC 4 cut(s) 53, 862, 1160, 1419
TseI GCWGC 7 cut(s) 13, 31, 73, 1262, 1768, 2015, 2287
Tsp45I GTSAC 4 cut(s) 53, 862, 1160, 1419
TspGWI ACGGA 3 cut(s) 16, 363, 2315
TspRI CASTG 6 cut(s) 252, 687, 1180, 1348, 1854, 1863
Van91I CCANNNNNTGG 1 cut(s) 1056
Vha464I CTTAAG 1 cut(s) 59
VpaK11BI GGWCC 6 cut(s) 139, 252, 902, 2159, 2243, 2268
VspI ATTAAT 1 cut(s) 2514
XapI RAATTY 5 cut(s) 144, 1024, 1280, 1762, 1852
XbaI TCTAGA 1 cut(s) 239
XceI RCATGY 1 cut(s) 1064
XhoI CTCGAG 1 cut(s) 932
XmiI GTMKAC 2 cut(s) 417, 1504
XmnI GAANNNNTTC 1 cut(s) 1134
XspI CTAG 7 cut(s) 209, 240, 291, 612, 1350, 1371, 1665
ZrmI AGTACT 1 cut(s) 616
Zsp2I ATGCAT 1 cut(s) 1311
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.