Rw5G009770
ERF Family

Probably involved in the RNA silencing pathway and required for the generation of small interfering RNAs (siRNAs)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000505
Physical Location & Seq
Reverse (-)
10569403 .. 10581177
11775 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G009770.1

Sequence Viewer

Length: 3069 bp
ATGGCTGCTGTGGAAAACTTTTATTGTCAGTTTCTTATTAGTTCACTTCCTTCTGAGCTTCACTCAATAGTTTCTGACCACCACACTCAACCCAAAGCAGCAGTGCCCTTCAACCAAGCAGCCATGTTCGGCGTGCAGCTACTCGACTCCGTGCAGCAATCGCTGGATCGAATATGTCACCTTAAGAAGCAACCGCAGCCGGACGACGGCATCAGAAGAAAGCTGGCATTGCTGAGTGAGCAAGACGCCCTCTACGTGCTGGACCAAATTTACAACAGCAAAACTGAGATCAGAACCCTCAATGGATTCATTGCTTTCATGATCAGGAAACTAGAGAATGAATCTTCTTCGTCTCCTTCTTCTAGAACAGTTTGGACCCCTCAGAATGAGACTGTCACTCACTCCCCAGTTCTATACCATCCTGGTGACCATGGACCAGCACACTTAGAAGCTCTGGGAGAACTTGAGTTCAGAAGACAATTTCTAATATTAAGTTATGCTGGAGGAATGAAGTTAAAAGATGTAATAGAAGTGGAGGCTATTAGGCGCTGGAGAAATTTGCCAATGGTAAACTTCGAGACCAAAGTTTGGCATGCTTTGGGTGAAAACAATGTTGGCAAGGCTGACCGACGATTGTATTCTGATTGGGATTCTCAAAGAACACACCACTACTATTGTTATGTTTCCGTGGATGGGAGTTGCAAGTTCAAGGGTCCCTTCCTAGACAAACCAAGAACTCTCCTACAGAAATGGTTAGAAGATGACAATGTTTTAGAGGTGAAATTTACCCAAGGGGAAGGTTCCAGTATTCTTTTTTATAATAATTATGGTACATATAGCAAGTTAATGCGAGAAGGCATTCTTGTCGGTTTGCGGCGATATGGTCACTTTGTGCACAGTGTTGGTGGTAACAAAGGAAAGAATAAGTTGAAAGACCCAACTTCATCACCTGTGAAATGTTACTTTGTTCGTATGAGCTCTGATGCAGCGATAGACATGGCTAAATTTTATAAGCTAGCTGGCAAAACGATGCATGAAGGAAGATCTCTTTTGACGCACGTACACACTGGGCCTAGTTTGGCTAATTACATGGCTAGGTTATCTCTCATTTTGTCCAAGACAGTTACTTTAGAAGTGAATTGGTCACTTGTCAAGGTTGAGTTCATCAATGAAGAATACTGTCTCGATGAGTCTGGTAATCCCATCTATGTAGATGGAAAAGCTTGTATACATACTGATGGCACGGGATTTATATCTGCGGATCTGGCTTTAATTTGTCCTGAGATAGCACACAAAGGAGAGCTTATCAATAATGAACACATCGAGGGACTTGATGATCCTGATGATGAACTTGAGGACAACATTCTGGAATGGAAACAGCCAGGCACTAGAACTCAGGAACTGCCTTTGCTCATGCAGTTCCGACTTTTTTACAAAGGCTATGCTATCAAAGGAACTTTTCTTGTCAATAAACTGCTTCCACCAAACACATTGCAGATTCGACCGTCAATGGTCAAAGTTGAGCCAGATCCAAAACTGTCAGATCCTCAAACAGTGAATTCACTGGAGATAGTAGGAACAAGTAAACTTACTAAGAGAACAACTTTGTCGAGGAACTTGATTGCACTTTTATACCATGGAGGGGTGCCAAAAGAATACTTCATCGAGTTACTTCTGAAAGCTTTAGAAGAGACTCGTGACGTTTATAGCAATAAACGTGCAGCATTCAGTGTTGCCCTTAGATATGGCGAGATCGATGACAATAGTAATGTTGCGAAAATGATCTCCTGTGGTATTCCTCTTGAAGAACCATATGTGCAATATCGTCTTTCAGTCCTTATGAACATGGAAAAGATCAATCTTAAAAAAGGGAAAATTTGTATTCCTGAATCATACTATTTGATGGGGACAGCTGATCCAACAGGAAAACTTGAAAAAGATCAAGTTTGCATTATCCTAAAAGATGGACAACTGTCGGGAATGGTTCTAGTGTACCGGAATCCTGGTGTACACTTCGGTGATATTCATGTCCTAAAAGCCACGTATGTCAAGGAACTAGAATCTGTTGTTGGAAATGCCAAGTACGGTATATTCTTCTCTTGTAAAGGTCCACGTTCCGTTGCGGATGAAATGGGTGGTGGAGATTTTGATGGTGATCTATACTGGATCTCAAGAAACCCTCAGCTGTTGGAATCGTTTAAACCAAGTGAACCTTGGATTGAAAAGTCATCTACCCAAAAAGTTCATGGTAAAAGACCAACCGAGCTTTCAAATGTTGATCTAGAAGATGAGCTCATCAAACTATTCTTGAAGACTAGGTTTGAACCAAGCTTCGCAATGAGTGAGGCAGCTCAACACTGGATGGCTTGGATGGACAAATTTTTGAGTTTAGCAGATACGAGTATTGATGAGAAAAACGAACTGAAGGCAAAGATACTGCAATTGATTGATATTTACTATGAAGCTCTCGATGCACCCAAGAAAGGTTTTAAGGTTGTAGTTCCAAAAGAATTGAAGGTGAAGACGTTTCCACATTTCATGGAGAAGAAACCCCTAGATTCCTACCATTCAATTTCCATTTTGGGATCAATTTATGATAAGGTGGATGAATATGAAACAGAAGACAGATCTCTCAAAGAAATTCAGAAACTTCCTCTGTTTGAAGTTGACGTTCCTGAGGGTAGCTTGAAAGAATGGAAGAAACTATACCAAGATTACCGGAATGAGATGACCTCTGCCATGGGAGCCGAAGATAAACAGGGCCAGAATCAGGCAGCCAATATCGTCATAAGAAAGTATAAAGAGATCCTATACAAGGCTGAGGAGTTTGAGCAAAGTGATATGCGGCACAGACAACAAATATATAATGACGCACTCGCTATTTATCACGTTTGTTATGACTATGCCAAGAGTGTTAGGACTGAAACGGGTGCACAAGATGTCAAGAAATGTGGCTTTGCTTGGAAAGTTGCAGGTGCAGCTCTATGCCAGCTTCACGAAGACTGGAGTTTGAAACCTGGTGAAAAGGTTTTCAGAGTCTTACCCACTGTTTTGAAGGACATTATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RdRP

Protein Analysis

1022

Amino Acids

116.37

Weight (kDa)

6.39

Isoelectric Point (pI)

39.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
4HB_RdRP3_N PF26249 47 - 110 2.3e-07 RdRP3/4/5, N-terminal four helical bundle domain
RdRP_helical PF26252 148 - 212 3.9e-06 RNA dependent RNA polymerase, helical domain
RdRP PF05183 235 - 868 3.9e-99 RNA dependent RNA polymerase
RdRP_head PF26253 896 - 1000 3.5e-09 RdRP, head domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000505)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G19910 AT2G19910 AT2G19920 AT2G19920 AT2G19920 AT2G19920 AT2G19930 AT2G19930 AT2G19930
fragaria_vesca FvH4_1g05980 FvH4_1g05990 FvH4_1g05990 FvH4_1g09718
malus_domestica MD02G1064400.v1.1 MD15G1195300.v1.1 MD15G1195400.v1.1
prunus_persica Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1
pyrus_communis pycom02g05070 pycom15g17460
rosa_chinensis RchiOBHm_Chr2g0091671 RchiOBHm_Chr2g0091681 RchiOBHm_Chr2g0091691 RchiOBHm_Chr2g0091701 RchiOBHm_Chr2g0091711 RchiOBHm_Chr2g0091721 RchiOBHm_Chr2g0091731 RchiOBHm_Chr5g0014401
rosa_laevigata RLG00000013772 RLG00000016242 RLG00000016244 RLG00000016245 RLG00000016246 RLG00000032105
rosa_multiflora Rmu_co8374041.1_g000001 Rmu_co8478121.1_g000001 Rmu_sc0000897.1_g000005 Rmu_sc0000897.1_g000009
rosa_roxburghii Rroxscaffold_1G00062180 Rroxscaffold_1G00062190 Rroxscaffold_1G00068800 Rroxscaffold_2G00149790 Rroxscaffold_2G00149800 Rroxscaffold_2G00149810
rosa_rugosa Rorug02G0019600 Rorug02G0019700 Rorug02G0019800 Rorug02G0019900 Rorug02G0019900 Rorug02G0020000 Rorug02G0020000 Rorug02G0020100 Rorug02G0020100 Rorug02G0020200 Rorug05G0017800
rosa_samantha Rh2AG064700 Rh2AG064800 Rh2AG064900 Rh2BG063800 Rh2BG063900 Rh2BG064000 Rh2BG064100 Rh2CG065500 Rh2CG065600 Rh2CG065700 Rh2DG063900 Rh2DG064000 Rh2DG064100 Rh2DG064200 Rh2DG381700 Rh5AG056700 Rh5AG056800 Rh5AG056900 Rh5AG112600 Rh5BG109300 Rh5CG063800 Rh5CG121100 Rh5DG053100 Rh5DG108300 Rh6CG100100
rosa_wichuraiana Rw2G005560 Rw2G005580 Rw2G005590 Rw5G005370 Rw5G009770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 819, 1011
AarI CACCTGC 1 cut(s) 2963
Acc36I ACCTGC 1 cut(s) 2963
AccB1I GGYRCC 1 cut(s) 1645
AccB7I CCANNNNNTGG 1 cut(s) 588
AccI GTMKAC 1 cut(s) 1228
AciI CCGC 5 cut(s) 194, 874, 1259, 2123, 2845
AclWI GGATC 9 cut(s) 174, 1269, 1331, 1523, 1538, 1910, 2174, 2593, 2800
AcsI RAATTY 8 cut(s) 267, 556, 782, 1004, 1558, 1875, 2378, 2640
AcuI CTGAAG 1 cut(s) 2444
AcyI GRCGYC 1 cut(s) 246
AdeI CACNNNGTG 1 cut(s) 892
AfaI GTAC 5 cut(s) 832, 1062, 1994, 2010, 2084
AfiI CCNNNNNNNGG 7 cut(s) 206, 588, 693, 1642, 2483, 2770, 2815
AflII CTTAAG 1 cut(s) 182
AjnI CCWGG 4 cut(s) 421, 1381, 2002, 3016
AjuI GAANNNNNNNTTGG 6 cut(s) 597, 629, 2052, 2084, 2251, 2283
AleI CACNNNNGTG 1 cut(s) 2016
Alw21I GWGCWC 4 cut(s) 897, 980, 2295, 2935
Alw26I GTCTC 5 cut(s) 357, 383, 572, 1187, 1685
Alw44I GTGCAC 2 cut(s) 893, 2931
AlwI GGATC 9 cut(s) 174, 1269, 1331, 1523, 1538, 1910, 2174, 2593, 2800
AlwNI CAGNNNCTG 1 cut(s) 1402
AoxI GGCC 2 cut(s) 1070, 2761
ApaLI GTGCAC 2 cut(s) 893, 2931
ApoI RAATTY 8 cut(s) 267, 556, 782, 1004, 1558, 1875, 2378, 2640
Asp700I GAANNNNTTC 1 cut(s) 858
AspLEI GCGC 1 cut(s) 549
AspS9I GGNCC 7 cut(s) 262, 375, 434, 713, 1070, 2108, 2761
AsuHPI GGTGA 9 cut(s) 170, 437, 614, 790, 939, 2030, 2165, 2530, 3032
AsuNHI GCTAGC 1 cut(s) 1015
AvaII GGWCC 5 cut(s) 262, 375, 434, 713, 2108
AxyI CCTNAGG 1 cut(s) 2676
BaeGI GKGCMC 3 cut(s) 108, 897, 2935
BanI GGYRCC 1 cut(s) 1645
BanII GRGCYC 2 cut(s) 980, 2295
BarI GAAGNNNNNNTAC 2 cut(s) 2690, 2722
BauI CACGAG 1 cut(s) 1695
BbsI GAAGAC 5 cut(s) 481, 2318, 2528, 2628, 3006
Bbv12I GWGCWC 4 cut(s) 897, 980, 2295, 2935
BbvCI CCTCAGC 2 cut(s) 2181, 2820
BceAI ACGGC 1 cut(s) 223
BcgI CGANNNNNNTGC 2 cut(s) 847, 881
BciT130I CCWGG 4 cut(s) 423, 1383, 2004, 3018
BclI TGATCA 1 cut(s) 321
BcoDI GTCTC 5 cut(s) 357, 383, 572, 1187, 1685
BfmI CTRYAG 1 cut(s) 743
BfoI RGCGCY 1 cut(s) 550
BfrI CTTAAG 1 cut(s) 182
BfuAI ACCTGC 1 cut(s) 2963
BglII AGATCT 2 cut(s) 1043, 2627
Bme1390I CCNGG 4 cut(s) 423, 1383, 2004, 3018
Bme18I GGWCC 5 cut(s) 262, 375, 434, 713, 2108
BmgT120I GGNCC 7 cut(s) 262, 375, 434, 713, 1070, 2108, 2761
BmiI GGNNCC 6 cut(s) 377, 714, 715, 802, 1647, 2746
BmrFI CCNGG 4 cut(s) 423, 1383, 2004, 3018
BmrI ACTGGG 2 cut(s) 401, 1077
BmsI GCATC 4 cut(s) 219, 973, 1020, 2461
BmtI GCTAGC 1 cut(s) 1019
BmuI ACTGGG 2 cut(s) 401, 1077
BoxI GACNNNNGTC 1 cut(s) 1972
BpiI GAAGAC 5 cut(s) 481, 2318, 2528, 2628, 3006
BplI GAGNNNNNCTC 4 cut(s) 47, 79, 2717, 2749
BpmI CTGGAG 4 cut(s) 522, 571, 1586, 3025
Bpu10I CCTNAGC 2 cut(s) 2181, 2820
BpuEI CTTGAG 3 cut(s) 485, 1373, 2155
Bsa29I ATCGAT 1 cut(s) 1755
BsaAI YACGTR 3 cut(s) 256, 1060, 2043
BsaBI GATNNNNATC 2 cut(s) 1252, 2154
BsaHI GRCGYC 1 cut(s) 246
BsaI GGTCTC 1 cut(s) 572
BsaJI CCNNGG 6 cut(s) 430, 687, 790, 1636, 2213, 2739
BsaWI WCCGGW 2 cut(s) 1995, 2718
Bsc4I CCNNNNNNNGG 7 cut(s) 206, 588, 693, 1642, 2483, 2770, 2815
Bse1I ACTGG 7 cut(s) 407, 804, 1072, 1569, 2168, 2363, 3008
Bse21I CCTNAGG 1 cut(s) 2676
Bse3DI GCAATG 4 cut(s) 227, 309, 1490, 2343
Bse8I GATNNNNATC 2 cut(s) 1252, 2154
BseBI CCWGG 4 cut(s) 423, 1383, 2004, 3018
BseCI ATCGAT 1 cut(s) 1755
BseDI CCNNGG 6 cut(s) 430, 687, 790, 1636, 2213, 2739
BseGI GGATG 6 cut(s) 418, 697, 2131, 2367, 2376, 2611
BseJI GATNNNNATC 2 cut(s) 1252, 2154
BseLI CCNNNNNNNGG 7 cut(s) 206, 588, 693, 1642, 2483, 2770, 2815
BseMI GCAATG 4 cut(s) 227, 309, 1490, 2343
BseMII CTCAG 9 cut(s) 45, 224, 276, 395, 1272, 1409, 2195, 2667, 2811
BseNI ACTGG 7 cut(s) 407, 804, 1072, 1569, 2168, 2363, 3008
BseRI GAGGAG 1 cut(s) 2837
BseSI GKGCMC 3 cut(s) 108, 897, 2935
BsgI GTGCAG 4 cut(s) 155, 173, 1740, 2997
Bsh1285I CGRYCG 1 cut(s) 1505
BshFI GGCC 2 cut(s) 1072, 2763
BshNI GGYRCC 1 cut(s) 1645
BshVI ATCGAT 1 cut(s) 1755
BsiEI CGRYCG 1 cut(s) 1505
BsiHKAI GWGCWC 4 cut(s) 897, 980, 2295, 2935
BsiSI CCGG 3 cut(s) 200, 1996, 2719
BslFI GGGAC 3 cut(s) 699, 1341, 1921
BslI CCNNNNNNNGG 7 cut(s) 206, 588, 693, 1642, 2483, 2770, 2815
BsmAI GTCTC 5 cut(s) 357, 383, 572, 1187, 1685
BsmBI CGTCTC 1 cut(s) 357
BsmFI GGGAC 3 cut(s) 699, 1341, 1921
BsmI GAATGC 2 cut(s) 858, 1724
BsnI GGCC 2 cut(s) 1072, 2763
Bso31I GGTCTC 1 cut(s) 572
Bsp1286I GDGCHC 5 cut(s) 108, 897, 980, 2295, 2935
Bsp1407I TGTACA 1 cut(s) 2008
Bsp19I CCATGG 3 cut(s) 430, 1636, 2739
BspACI CCGC 5 cut(s) 194, 874, 1259, 2123, 2845
BspANI GGCC 2 cut(s) 1072, 2763
BspCNI CTCAG 9 cut(s) 46, 225, 277, 394, 1273, 1408, 2194, 2668, 2812
BspDI ATCGAT 1 cut(s) 1755
BspHI TCATGA 1 cut(s) 318
BspLI GGNNCC 6 cut(s) 377, 714, 715, 802, 1647, 2746
BspMI ACCTGC 1 cut(s) 2963
BspOI GCTAGC 1 cut(s) 1019
BspPI GGATC 9 cut(s) 174, 1269, 1331, 1523, 1538, 1910, 2174, 2593, 2800
BspT107I GGYRCC 1 cut(s) 1645
BspTI CTTAAG 1 cut(s) 182
BspTNI GGTCTC 1 cut(s) 572
BsrDI GCAATG 4 cut(s) 227, 309, 1490, 2343
BsrGI TGTACA 1 cut(s) 2008
BsrI ACTGG 7 cut(s) 407, 804, 1072, 1569, 2168, 2363, 3008
BssECI CCNNGG 6 cut(s) 430, 687, 790, 1636, 2213, 2739
BssNAI GTATAC 1 cut(s) 1229
BssNI GRCGYC 1 cut(s) 246
BssSI CACGAG 1 cut(s) 1695
BssT1I CCWWGG 5 cut(s) 430, 790, 1636, 2213, 2739
Bst1107I GTATAC 1 cut(s) 1229
Bst2BI CACGAG 1 cut(s) 1695
Bst2UI CCWGG 4 cut(s) 423, 1383, 2004, 3018
Bst6I CTCTTC 1 cut(s) 1683
BstACI GRCGYC 1 cut(s) 246
BstAFI CTTAAG 1 cut(s) 182
BstAUI TGTACA 1 cut(s) 2008
BstBAI YACGTR 3 cut(s) 256, 1060, 2043
BstC8I GCNNGC 6 cut(s) 134, 225, 594, 1017, 1021, 2990
BstDSI CCRYGG 4 cut(s) 430, 687, 1636, 2739
BstEII GGTNACC 1 cut(s) 425
BstENI CCTNNNNNAGG 1 cut(s) 2813
BstF5I GGATG 6 cut(s) 418, 697, 2131, 2367, 2376, 2611
BstH2I RGCGCY 1 cut(s) 550
BstHHI GCGC 1 cut(s) 549
BstMAI GTCTC 5 cut(s) 357, 383, 572, 1187, 1685
BstMCI CGRYCG 1 cut(s) 1505
BstMWI GCNNNNNNNGC 8 cut(s) 160, 196, 229, 238, 1265, 2471, 2744, 2978
BstNI CCWGG 4 cut(s) 423, 1383, 2004, 3018
BstNSI RCATGY 1 cut(s) 596
BstPAI GACNNNNGTC 1 cut(s) 1972
BstPI GGTNACC 1 cut(s) 425
BstSCI CCNGG 4 cut(s) 421, 1381, 2002, 3016
BstSFI CTRYAG 1 cut(s) 743
BstSLI GKGCMC 3 cut(s) 108, 897, 2935
BstV2I GAAGAC 5 cut(s) 481, 2318, 2528, 2628, 3006
BstX2I RGATCY 7 cut(s) 1043, 1261, 1528, 1543, 2166, 2627, 2805
BstYI RGATCY 7 cut(s) 1043, 1261, 1528, 1543, 2166, 2627, 2805
BstZ17I GTATAC 1 cut(s) 1229
Bsu15I ATCGAT 1 cut(s) 1755
Bsu36I CCTNAGG 1 cut(s) 2676
BsuRI GGCC 2 cut(s) 1072, 2763
BsuTUI ATCGAT 1 cut(s) 1755
BtgI CCRYGG 4 cut(s) 430, 687, 1636, 2739
BtsCI GGATG 6 cut(s) 418, 697, 2131, 2367, 2376, 2611
BtsI GCAGTG 1 cut(s) 108
BtsIMutI CAGTG 8 cut(s) 108, 904, 1065, 1560, 1562, 1735, 2356, 3045
BveI ACCTGC 1 cut(s) 2963
Cac8I GCNNGC 6 cut(s) 134, 225, 594, 1017, 1021, 2990
CaiI CAGNNNCTG 1 cut(s) 1402
CciI TCATGA 1 cut(s) 318
CfoI GCGC 1 cut(s) 549
Cfr13I GGNCC 7 cut(s) 262, 375, 434, 713, 1070, 2108, 2761
ClaI ATCGAT 1 cut(s) 1755
CseI GACGC 3 cut(s) 254, 1063, 2879
CsiI ACCWGGT 1 cut(s) 3016
Csp6I GTAC 5 cut(s) 831, 1061, 1993, 2009, 2083
CspCI CAANNNNNGTGG 4 cut(s) 656, 691, 2932, 2967
CviQI GTAC 5 cut(s) 831, 1061, 1993, 2009, 2083
DraI TTTAAA 1 cut(s) 2200
DraIII CACNNNGTG 1 cut(s) 892
Eam1104I CTCTTC 1 cut(s) 1683
EarI CTCTTC 1 cut(s) 1683
Ecl136II GAGCTC 2 cut(s) 978, 2293
Eco130I CCWWGG 5 cut(s) 430, 790, 1636, 2213, 2739
Eco24I GRGCYC 2 cut(s) 980, 2295
Eco31I GGTCTC 1 cut(s) 572
Eco47I GGWCC 5 cut(s) 262, 375, 434, 713, 2108
Eco53kI GAGCTC 2 cut(s) 978, 2293
Eco57I CTGAAG 1 cut(s) 2444
Eco81I CCTNAGG 1 cut(s) 2676
Eco91I GGTNACC 1 cut(s) 425
EcoICRI GAGCTC 2 cut(s) 978, 2293
EcoNI CCTNNNNNAGG 1 cut(s) 2813
EcoO109I RGGNCCY 1 cut(s) 713
EcoO65I GGTNACC 1 cut(s) 425
EcoRI GAATTC 1 cut(s) 1558
EcoRII CCWGG 4 cut(s) 421, 1381, 2002, 3016
EcoT14I CCWWGG 5 cut(s) 430, 790, 1636, 2213, 2739
EcoT22I ATGCAT 1 cut(s) 1035
EcoT38I GRGCYC 2 cut(s) 980, 2295
ErhI CCWWGG 5 cut(s) 430, 790, 1636, 2213, 2739
Esp3I CGTCTC 1 cut(s) 357
FalI AAGNNNNNCTT 8 cut(s) 701, 733, 846, 878, 1287, 1319, 2197, 2229
FaqI GGGAC 3 cut(s) 699, 1341, 1921
FauNDI CATATG 1 cut(s) 1813
FbaI TGATCA 1 cut(s) 321
FblI GTMKAC 1 cut(s) 1228
FokI GGATG 6 cut(s) 405, 704, 2138, 2374, 2383, 2618
FriOI GRGCYC 2 cut(s) 980, 2295
GlaI GCGC 1 cut(s) 548
GsuI CTGGAG 4 cut(s) 522, 571, 1586, 3025
HaeII RGCGCY 1 cut(s) 550
HaeIII GGCC 2 cut(s) 1072, 2763
HapII CCGG 3 cut(s) 200, 1996, 2719
HgaI GACGC 3 cut(s) 254, 1063, 2879
HhaI GCGC 1 cut(s) 549
Hin1I GRCGYC 1 cut(s) 246
Hin6I GCGC 1 cut(s) 547
HinP1I GCGC 1 cut(s) 547
HincII GTYRAC 1 cut(s) 2668
HindII GTYRAC 1 cut(s) 2668
HindIII AAGCTT 3 cut(s) 1221, 1680, 2329
HpaII CCGG 3 cut(s) 200, 1996, 2719
HphI GGTGA 9 cut(s) 170, 437, 614, 790, 939, 2030, 2165, 2530, 3032
Hpy99I CGWCG 2 cut(s) 209, 633
HpyCH4IV ACGT 9 cut(s) 255, 1059, 1701, 1717, 2042, 2113, 2525, 2670, 2889
HpyF10VI GCNNNNNNNGC 8 cut(s) 160, 196, 229, 238, 1265, 2471, 2744, 2978
HpySE526I ACGT 9 cut(s) 255, 1059, 1701, 1717, 2042, 2113, 2525, 2670, 2889
Hsp92I GRCGYC 1 cut(s) 246
HspAI GCGC 1 cut(s) 547
KflI GGGWCCC 1 cut(s) 713
Ksp22I TGATCA 1 cut(s) 321
LmnI GCTCC 1 cut(s) 2744
LweI GCATC 4 cut(s) 219, 973, 1020, 2461
MabI ACCWGGT 1 cut(s) 3016
MaeII ACGT 9 cut(s) 255, 1059, 1701, 1717, 2042, 2113, 2525, 2670, 2889
MfeI CAATTG 1 cut(s) 2441
MflI RGATCY 7 cut(s) 1043, 1261, 1528, 1543, 2166, 2627, 2805
MhlI GDGCHC 5 cut(s) 108, 897, 980, 2295, 2935
MlyI GAGTC 4 cut(s) 140, 1199, 1687, 3045
MmeI TCCRAC 4 cut(s) 1447, 1943, 2050, 2169
Mph1103I ATGCAT 1 cut(s) 1035
MroXI GAANNNNTTC 1 cut(s) 858
MseI TTAA 8 cut(s) 183, 491, 515, 845, 1271, 1863, 2199, 2490
MslI CAYNNNNRTG 3 cut(s) 423, 1236, 2016
MspA1I CMGCKG 2 cut(s) 1913, 2185
MspCI CTTAAG 1 cut(s) 182
MspI CCGG 3 cut(s) 200, 1996, 2719
MspR9I CCNGG 4 cut(s) 423, 1383, 2004, 3018
MssI GTTTAAAC 1 cut(s) 2200
MunI CAATTG 1 cut(s) 2441
Mva1269I GAATGC 2 cut(s) 858, 1724
MvaI CCWGG 4 cut(s) 423, 1383, 2004, 3018
MwoI GCNNNNNNNGC 8 cut(s) 160, 196, 229, 238, 1265, 2471, 2744, 2978
NcoI CCATGG 3 cut(s) 430, 1636, 2739
NdeI CATATG 1 cut(s) 1813
NheI GCTAGC 1 cut(s) 1015
NlaIV GGNNCC 6 cut(s) 377, 714, 715, 802, 1647, 2746
NmuCI GTSAC 6 cut(s) 176, 394, 425, 884, 1143, 1697
NsiI ATGCAT 1 cut(s) 1035
NspI RCATGY 1 cut(s) 596
OliI CACNNNNGTG 1 cut(s) 2016
PaeI GCATGC 1 cut(s) 596
PagI TCATGA 1 cut(s) 318
PaqCI CACCTGC 1 cut(s) 2963
PcsI WCGNNNNNNNCGW 1 cut(s) 252
PctI GAATGC 2 cut(s) 858, 1724
PdmI GAANNNNTTC 1 cut(s) 858
PflMI CCANNNNNTGG 1 cut(s) 588
PleI GAGTC 4 cut(s) 140, 1198, 1687, 3044
PmeI GTTTAAAC 1 cut(s) 2200
PpsI GAGTC 4 cut(s) 140, 1198, 1687, 3044
Ppu21I YACGTR 3 cut(s) 256, 1060, 2043
PpuMI RGGWCCY 1 cut(s) 713
PshAI GACNNNNGTC 1 cut(s) 1972
PsiI TTATAA 2 cut(s) 819, 1011
Psp124BI GAGCTC 2 cut(s) 980, 2295
Psp5II RGGWCCY 1 cut(s) 713
Psp6I CCWGG 4 cut(s) 421, 1381, 2002, 3016
PspEI GGTNACC 1 cut(s) 425
PspGI CCWGG 4 cut(s) 421, 1381, 2002, 3016
PspN4I GGNNCC 6 cut(s) 377, 714, 715, 802, 1647, 2746
PspPI GGNCC 7 cut(s) 262, 375, 434, 713, 1070, 2108, 2761
PspPPI RGGWCCY 1 cut(s) 713
PstNI CAGNNNCTG 1 cut(s) 1402
PsuI RGATCY 7 cut(s) 1043, 1261, 1528, 1543, 2166, 2627, 2805
PvuII CAGCTG 2 cut(s) 1913, 2185
RsaI GTAC 5 cut(s) 832, 1062, 1994, 2010, 2084
RsaNI GTAC 5 cut(s) 831, 1061, 1993, 2009, 2083
RseI CAYNNNNRTG 3 cut(s) 423, 1236, 2016
SacI GAGCTC 2 cut(s) 980, 2295
SaqAI TTAA 8 cut(s) 183, 491, 515, 845, 1271, 1863, 2199, 2490
Sau96I GGNCC 7 cut(s) 262, 375, 434, 713, 1070, 2108, 2761
SchI GAGTC 4 cut(s) 140, 1199, 1687, 3045
ScrFI CCNGG 4 cut(s) 423, 1383, 2004, 3018
SduI GDGCHC 5 cut(s) 108, 897, 980, 2295, 2935
SexAI ACCWGGT 1 cut(s) 3016
SfaNI GCATC 4 cut(s) 219, 973, 1020, 2461
SfcI CTRYAG 1 cut(s) 743
SinI GGWCC 5 cut(s) 262, 375, 434, 713, 2108
SmiMI CAYNNNNRTG 3 cut(s) 423, 1236, 2016
SmlI CTYRAG 4 cut(s) 182, 464, 1352, 2170
SmoI CTYRAG 4 cut(s) 182, 464, 1352, 2170
SphI GCATGC 1 cut(s) 596
SsiI CCGC 5 cut(s) 194, 874, 1259, 2123, 2845
SspI AATATT 1 cut(s) 489
SstI GAGCTC 2 cut(s) 980, 2295
StyD4I CCNGG 4 cut(s) 421, 1381, 2002, 3016
StyI CCWWGG 5 cut(s) 430, 790, 1636, 2213, 2739
TaiI ACGT 9 cut(s) 258, 1062, 1704, 1720, 2045, 2116, 2528, 2673, 2892
TaqII GACCGA 1 cut(s) 642
TatI WGTACW 1 cut(s) 2008
TauI GCSGC 2 cut(s) 877, 2848
Tru1I TTAA 8 cut(s) 183, 491, 515, 845, 1271, 1863, 2199, 2490
Tru9I TTAA 8 cut(s) 183, 491, 515, 845, 1271, 1863, 2199, 2490
TscAI CASTG 8 cut(s) 108, 904, 1072, 1560, 1569, 1735, 2363, 3052
TseFI GTSAC 6 cut(s) 176, 394, 425, 884, 1143, 1697
Tsp45I GTSAC 6 cut(s) 176, 394, 425, 884, 1143, 1697
TspGWI ACGGA 3 cut(s) 139, 676, 2107
TspRI CASTG 8 cut(s) 108, 904, 1072, 1560, 1569, 1735, 2363, 3052
Van91I CCANNNNNTGG 1 cut(s) 588
Vha464I CTTAAG 1 cut(s) 182
VneI GTGCAC 2 cut(s) 893, 2931
VpaK11BI GGWCC 5 cut(s) 262, 375, 434, 713, 2108
XagI CCTNNNNNAGG 1 cut(s) 2813
XapI RAATTY 8 cut(s) 267, 556, 782, 1004, 1558, 1875, 2378, 2640
XbaI TCTAGA 2 cut(s) 362, 2281
XceI RCATGY 1 cut(s) 596
XcmI CCANNNNNNNNNTGG 2 cut(s) 2211, 2243
XmiI GTMKAC 1 cut(s) 1228
XmnI GAANNNNTTC 1 cut(s) 858
Zsp2I ATGCAT 1 cut(s) 1035
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.