Rw2G005580
ERF Family

Probably involved in the RNA silencing pathway and required for the generation of small interfering RNAs (siRNAs)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000505
Physical Location & Seq
Reverse (-)
5253623 .. 5268863
15241 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G005580.1

Sequence Viewer

Length: 4065 bp
ATGATGAAGCAGAGTTGGGATGAGCAAGAAGAGGTGCCGCGTCTACCTCAGAAAGTGGAGGTATTGCTCCGGCAAATCTGTGAGGACAAAAAGCAACCTTGGCCGGACGCTGACGCCATAAGGCAGCTCGCGGCTCAGAGCGAGGAGGCGGCAATGGAGACCCTGAAGAAAATCTCCACCAGCAAAAGCATCAGAACCACCCTCGGCCGTTACATCAAGTGGATGATCGGGCAATGTAGCTCTGCTTCTCCGTCGCCGTCCAAAACGGTGCGTTCCGCTCACGTTCAAAGCCCTGGTGCTACTTCACTCACTCCTCAGAGACTTCACTTCAGCCCTCCAGGTGAGGCAGTTAGTGACAGGTCACCTGTTGTTGGGCAATTACCTTATTGCTCTGGTTCTCCATCCAAAGCCGCGGGGACTTGTTCTTATCAAAGATTGTCTGCTCATGCACAGGTTGATGAGAGGTCTCGAGGCGAGGCATTTAATCAGCTGTCACCTGTTATTGGTCAATCACCTTATTCCTCTGGTTCTCCTTTGAAAGCCGCAGACACTTCTTCTTATCAAAGACTGTCTGCTCCTGTACAACTGGATGAGAGATTTCAAGGTGATGCATTTAGTCATTGGTCATCTGTTGGACAACCACCTTATTCCTCTGCTTCTCCATCAAAAGCTGCAGGCACTACTTCTTACCAAAGATTGTCTCCTCCTGTACAACTTGATGATACGTTTCAAGGGGAGGCATTTACTGTTCTAGAGCAACCAGGAAACATCAATAATGGACAATCACCTTATTATTCCTCTGGTTCTCCATTGAAAGTGGCACGCACTTGTTCGTGTCAAAGACCATCTGCGTTGGTTGCTCCTATACAACTTGATAAGAAGTTTCAAGGTGACGCATTTACTGACAGGTCACTTGTTCAACAATTACCTTGTTCCTCTGGTTCTCCATTAAAAGCCACTGGTTCTTATGAAAGATTGTCTACTCCTGCACAAGCTGATGAGCGGTTTCATGGTGAGGCATTTAGTGCCCGGTCAGCTGTTGGACAATTGCCTTGTTCCTCTGTTTCTCCATTAAAATCTGCAGTCACTTCTTCTTATCAAAGATTGTCTACTCCTGTACAGCTTGATGAGAGGTTTCAAGTTGAGGCATTTAATGACCGGTCACCTGTTATTGGACAATCACCTTACTCTGGTTCTCCATTGAAAGCCGCAAGCACTTCTTATCAAAGACTGACTGCTCCTCTACAACTTGATGAGAGGGTTCAAGGTGGGGTATTTGCTGATCAGACACCTGTTCAAGGACAATCACCATATGCCTCTTGTTCTCCATCAAAAGCCTTCTGCATTTCTCCTTATCAGAGAATGTCTGCTGATGTTGTTCCTGTACAACTTCATGAAAGGTTTCAAGGTGAGGGTATCACTGGGTCCTCAGCTATAGACAAACCTGGAGATCATGGAGAAGCACACTTAGTAGCTCTGGGTGAACTAGAATTCCGTAAACAATTTCTGATTTTAAGTTATGCTGGAGGAACGAAGTTGAGAGATCTTATAGCGGCAGAGGACATTAGGGATTGGAAATATTTGCCAATGGTAATGTTTGAGAGCAAAATTTGGGAGTATCTAGGTCGAAAATGCATCAGCCTCGAAGACCGTCGATTGAATTTTGATTGGGATTCTGGACGCACACACGTCTATCATTGTTACGTCAACGTGGATGGGAGTTACATATTTAAGGGCCCATATTTATATAAAATAAGAAGAACGCTCCTACAGAAAAAATTAGGAGATGACAATGTTTTAATGGTGAAGTTTGCCAAAGAAGAGGTGCAGAGAGGTCAAAGTATTGCCTGCCATCATAATTATTCTATGTATATGAAGGTTGCAAGAGAAGGGATTCTTGTTGGTTTGCGGCGATATCGTTTCTTTGTGTTCAAAGATGGTGGTAATGAGGAGAAGAAAAAAAACCCAACTTCATCACCTGTGAAGTGTTACTTTGTTCGAGTGACCTCTGATGCAGCCATAGACAAAGGTGTCAGTTATATATTATCTGACAGAACGATGTATGAAGCCAGATGTCTTTTCATGCATGCACATACTCTGGCTAGTATTGATAACTACATGGTTAGGTTCTCACTCATTTTGTCCAAGACAACGAGCTTAAAAGTAGATTGGTCAGTTGTCAAGGTTGAAATCATCGGTGAAGAATACTGTCTCGATGAATCTGGTAATCGTATCCATAGAGATGAAAAACCACTTATACATACAGATGGAACTGGATTCATATCCGAGGATTTGGCTTTAATTTGTCCAAAGATAGAAGCCAAAGGACAATGTATAAGTGATGAACACATGAAGGGACTTCCTGATCCTGATGAACTTGAGGATAATGATATGGGAAAGAAAAGGCCAGTTCTTAGAACAGAAGAACCGCCTTTGCTCATACAGTGCCGACTTTTTCACAATGGCAGTGCTTCTAAAGGAACGTTTCTTGTCAATAAAAAGCTCCATCCGAACACAATCCAGATTCGACCATCCATGCTCAAAGTTAAGACAGATCCAACAATTTCCAATAATCAAACAATAAACTCATTGGAAATAGTTGGCACAAGCACTCCTCCTAGGAATACGCACTTATCGAGGATTTTAATTGCACTTCTAATGCATGGAGGGGTACCGGAAGAATTTTTCATGCAGATACTTAACCAAGCTTTGGAAGATGCTCATGGTGTTTTCTGCAAGACACGTGCAGCACTCAAAGTTGCTGAGAAGTATGGTGAGATTGATGACAATTTCTTGGGGAAATTGATGATCTCATTTGGAATACCTCTAGAAGAATCATACTTGCGGTATCGTCTCTCAGTCCTAATGAAAGAGGAAAACAACAGTCTTAGAAAAGGGAAACTTTATATTCCTGATTCCTATAATTTGATGGGGACAGCTGATCCAACAGGGACTTTAAAAAAAGATGAAGTCTGTGTTATCCTGAAGGATGGACAACTGTCAGGGAAGGTACTAGTCTACAGATATCCGGGTTTACACTTTGGGGATATTCATGTTCTAAAGGCGACCTATGTGAAGGAGTTAGAATCTTTTGTTGGAAATGCCAAATACGGTATATTCTTCTCTTGCAAAGGTCCACGTTCCATTGCTGATGAAATGGGTGGTGGAGATTTTGATGGTGATCTATACTGGGTCTCAAGAAACCCTCAGCTATTGGAGTGGTACAAACCAAGTGAACCTTGGATTGAAGCTTCAATACCAACCCAAAAAGTTCCCAGTAGGAGTCCATCTGACTATTCGCCTGAAGATCTAGAAGATCTTCTGTTTAGATCATTCTTGACAACGAGGTTTGAACCAAGTTTCGCTATGAGTGAGGCAGCTGATAATTGGCTAGCCTGGATGGATCGTTTCTTGACGTTGGGAGAGAGTGACATTGACGAAAAGAATAATATGAAGATGAAGATACTGCAACTAATTAACTTATACTACACAGCTCTAGATGCACCGAAGAAAGGTGTAAAGGTTGTAGTCCCAAAAGAATTGAAGGCCCCATGTTTCCCCCATTACATGGGAAAGAACAATTCTTACCCTTCAACTTCCATTCTGGGATTAATTTATAACAGGGTGGATGAGGATCAAACAGAAGACCGTTCTCTCAAAGAAGTCGAGAAACTTCCTGCTTTTGATGTTAATGAAGTCCCTGATGCCTGCTTGGACAAATGGGAGGAACTACACCGACAGTACAGGAGTGAGATGGCCTCTGCTATGGACGATGCTGATAAACAGAGCAAAAATCAGTCTGCCGATGTGGTCATAAGGAAATATAAAAAGATTCTTTACGAGGCTAATGACTTTGAGGAGAGTAGAAGGCCAATCGATGAGATATATAACGAGGCACAAGCTATATATCATGTGTGTTATGACCATGCGAGGAGGATAGGAAGTGTGAAGGCGTGTTCCTTTGCATGGAGAGTTGCAGGTAAAGCTCTGTGCCAGCTTCACGCCAAGAACACGAATGAAAGGTGTTGTACGATAGTATCCTCTGTTTTGAAGGAGATTTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RdRP

Protein Analysis

1354

Amino Acids

151.64

Weight (kDa)

7.13

Isoelectric Point (pI)

47.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RdRP_helical PF26252 489 - 553 4e-07 RNA dependent RNA polymerase, helical domain
RdRP PF05183 575 - 1209 5.4e-100 RNA dependent RNA polymerase
RdRP_head PF26253 1228 - 1338 4.2e-14 RdRP, head domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000505)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G19910 AT2G19910 AT2G19920 AT2G19920 AT2G19920 AT2G19920 AT2G19930 AT2G19930 AT2G19930
fragaria_vesca FvH4_1g05980 FvH4_1g05990 FvH4_1g05990 FvH4_1g09718
malus_domestica MD02G1064400.v1.1 MD15G1195300.v1.1 MD15G1195400.v1.1
prunus_persica Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1
pyrus_communis pycom02g05070 pycom15g17460
rosa_chinensis RchiOBHm_Chr2g0091671 RchiOBHm_Chr2g0091681 RchiOBHm_Chr2g0091691 RchiOBHm_Chr2g0091701 RchiOBHm_Chr2g0091711 RchiOBHm_Chr2g0091721 RchiOBHm_Chr2g0091731 RchiOBHm_Chr5g0014401
rosa_laevigata RLG00000013772 RLG00000016242 RLG00000016244 RLG00000016245 RLG00000016246 RLG00000032105
rosa_multiflora Rmu_co8374041.1_g000001 Rmu_co8478121.1_g000001 Rmu_sc0000897.1_g000005 Rmu_sc0000897.1_g000009
rosa_roxburghii Rroxscaffold_1G00062180 Rroxscaffold_1G00062190 Rroxscaffold_1G00068800 Rroxscaffold_2G00149790 Rroxscaffold_2G00149800 Rroxscaffold_2G00149810
rosa_rugosa Rorug02G0019600 Rorug02G0019700 Rorug02G0019800 Rorug02G0019900 Rorug02G0019900 Rorug02G0020000 Rorug02G0020000 Rorug02G0020100 Rorug02G0020100 Rorug02G0020200 Rorug05G0017800
rosa_samantha Rh2AG064700 Rh2AG064800 Rh2AG064900 Rh2BG063800 Rh2BG063900 Rh2BG064000 Rh2BG064100 Rh2CG065500 Rh2CG065600 Rh2CG065700 Rh2DG063900 Rh2DG064000 Rh2DG064100 Rh2DG064200 Rh2DG381700 Rh5AG056700 Rh5AG056800 Rh5AG056900 Rh5AG112600 Rh5BG109300 Rh5CG063800 Rh5CG121100 Rh5DG053100 Rh5DG108300 Rh6CG100100
rosa_wichuraiana Rw2G005560 Rw2G005580 Rw2G005590 Rw5G005370 Rw5G009770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3618
AasI GACNNNNNNGTC 1 cut(s) 2030
Acc36I ACCTGC 1 cut(s) 3968
Acc65I GGTACC 1 cut(s) 2671
AccB1I GGYRCC 2 cut(s) 34, 2671
AccB7I CCANNNNNTGG 2 cut(s) 2710, 3568
AccBSI CCGCTC 2 cut(s) 278, 1003
AccI GTMKAC 4 cut(s) 43, 980, 1109, 3018
AccII CGCG 3 cut(s) 40, 131, 413
AclI AACGTT 1 cut(s) 2483
AclWI GGATC 5 cut(s) 2360, 2549, 2936, 3411, 3642
AcoI YGGCCR 2 cut(s) 101, 205
AcsI RAATTY 4 cut(s) 1490, 1608, 1660, 2681
AcuI CTGAAG 4 cut(s) 185, 313, 3005, 3324
AcvI CACGTG 1 cut(s) 2744
AcyI GRCGYC 1 cut(s) 114
AfaI GTAC 9 cut(s) 582, 711, 1119, 1386, 2673, 3012, 3224, 3743, 4030
AfiI CCNNNNNNNGG 9 cut(s) 371, 503, 1172, 1190, 1298, 2710, 3512, 3568, 3966
AflIII ACRYGT 2 cut(s) 1687, 2741
AgeI ACCGGT 1 cut(s) 1158
AhlI ACTAGT 1 cut(s) 3013
AjiI CACGTC 1 cut(s) 1690
AjnI CCWGG 5 cut(s) 292, 337, 760, 1444, 3395
AjuI GAANNNNNNNTTGG 2 cut(s) 3078, 3110
AloI GAACNNNNNNTCC 2 cut(s) 1476, 1508
Alw26I GTCTC 7 cut(s) 152, 313, 471, 705, 2216, 2858, 3199
AlwI GGATC 5 cut(s) 2360, 2549, 2936, 3411, 3642
Ama87I CYCGRG 1 cut(s) 468
AoxI GGCC 7 cut(s) 101, 205, 1735, 2405, 3546, 3756, 3869
ApaI GGGCCC 1 cut(s) 1739
ApeKI GCWGC 5 cut(s) 124, 671, 2015, 2747, 3377
ApoI RAATTY 4 cut(s) 1490, 1608, 1660, 2681
AseI ATTAAT 1 cut(s) 3611
AsiGI ACCGGT 1 cut(s) 1158
Asp700I GAANNNNTTC 3 cut(s) 1401, 1893, 3318
Asp718I GGTACC 1 cut(s) 2671
AspA2I CCTAGG 1 cut(s) 2618
AspS9I GGNCC 5 cut(s) 1425, 1735, 1736, 3134, 3547
AsuC2I CCSGG 2 cut(s) 1030, 3030
AsuNHI GCTAGC 1 cut(s) 3391
AvaI CYCGRG 1 cut(s) 468
AvaII GGWCC 2 cut(s) 1425, 3134
AvrII CCTAGG 1 cut(s) 2618
BaeGI GKGCMC 2 cut(s) 1030, 1739
BaeI ACNNNNGTAYC 2 cut(s) 4020, 4053
BanI GGYRCC 2 cut(s) 34, 2671
BanII GRGCYC 1 cut(s) 1739
BarI GAAGNNNNNNTAC 4 cut(s) 229, 261, 3240, 3272
BbrPI CACGTG 1 cut(s) 2744
BbsI GAAGAC 2 cut(s) 1653, 3651
BbvCI CCTCAGC 2 cut(s) 1429, 3207
BbvI GCAGC 5 cut(s) 136, 658, 2027, 2759, 3389
BceAI ACGGC 2 cut(s) 192, 241
BcgI CGANNNNNNTGC 2 cut(s) 1624, 1658
BciT130I CCWGG 5 cut(s) 294, 339, 762, 1446, 3397
BciVI GTATCC 2 cut(s) 2243, 4048
BclI TGATCA 1 cut(s) 1282
BcnI CCSGG 2 cut(s) 1030, 3030
BcoDI GTCTC 7 cut(s) 152, 313, 471, 705, 2216, 2858, 3199
BcuI ACTAGT 1 cut(s) 3013
BfmI CTRYAG 5 cut(s) 672, 1080, 1434, 1769, 3019
BfuAI ACCTGC 1 cut(s) 3968
BfuI GTATCC 2 cut(s) 2243, 4048
BglII AGATCT 3 cut(s) 1543, 3307, 3316
BlnI CCTAGG 1 cut(s) 2618
Bme1390I CCNGG 7 cut(s) 294, 339, 762, 1030, 1446, 3030, 3397
Bme18I GGWCC 2 cut(s) 1425, 3134
BmeT110I CYCGRG 1 cut(s) 468
BmgBI CACGTC 1 cut(s) 1690
BmgT120I GGNCC 5 cut(s) 1425, 1735, 1736, 3134, 3547
BmiI GGNNCC 5 cut(s) 36, 1426, 1737, 2673, 3549
BmrFI CCNGG 7 cut(s) 294, 339, 762, 1030, 1446, 3030, 3397
BmrI ACTGGG 3 cut(s) 1431, 3199, 3270
BmsI GCATC 8 cut(s) 198, 598, 1644, 2002, 2707, 3490, 3694, 3763
BmtI GCTAGC 1 cut(s) 3395
BmuI ACTGGG 3 cut(s) 1431, 3199, 3270
BoxI GACNNNNGTC 1 cut(s) 2998
BpiI GAAGAC 2 cut(s) 1653, 3651
BplI GAGNNNNNCTC 2 cut(s) 3743, 3775
BpmI CTGGAG 3 cut(s) 321, 1467, 1545
Bpu10I CCTNAGC 2 cut(s) 1429, 3207
BpuEI CTTGAG 2 cut(s) 2399, 3181
BpuMI CCSGG 2 cut(s) 1030, 3030
Bsa29I ATCGAT 1 cut(s) 3876
BsaAI YACGTR 1 cut(s) 2744
BsaBI GATNNNNATC 3 cut(s) 2281, 3180, 3633
BsaHI GRCGYC 1 cut(s) 114
BsaI GGTCTC 3 cut(s) 152, 471, 3199
BsaJI CCNNGG 7 cut(s) 98, 202, 292, 411, 2286, 2618, 3239
BsaWI WCCGGW 2 cut(s) 1158, 2674
BsaXI ACNNNNNCTCC 3 cut(s) 2596, 2626, 4046
Bsc4I CCNNNNNNNGG 9 cut(s) 371, 503, 1172, 1190, 1298, 2710, 3512, 3568, 3966
Bse118I RCCGGY 1 cut(s) 1158
Bse1I ACTGG 7 cut(s) 591, 964, 1426, 2278, 2408, 3194, 3276
Bse3DI GCAATG 3 cut(s) 159, 239, 3144
Bse8I GATNNNNATC 3 cut(s) 2281, 3180, 3633
BseBI CCWGG 5 cut(s) 294, 339, 762, 1446, 3397
BseCI ATCGAT 1 cut(s) 3876
BseDI CCNNGG 7 cut(s) 98, 202, 292, 411, 2286, 2618, 3239
BseJI GATNNNNATC 3 cut(s) 2281, 3180, 3633
BseLI CCNNNNNNNGG 9 cut(s) 371, 503, 1172, 1190, 1298, 2710, 3512, 3568, 3966
BseMI GCAATG 3 cut(s) 159, 239, 3144
BseMII CTCAG 7 cut(s) 62, 149, 329, 1443, 2754, 2871, 3221
BseNI ACTGG 7 cut(s) 591, 964, 1426, 2278, 2408, 3194, 3276
BseRI GAGGAG 8 cut(s) 158, 303, 693, 1230, 1964, 2604, 3872, 3946
BseSI GKGCMC 2 cut(s) 1030, 1739
BseX3I CGGCCG 1 cut(s) 205
BseXI GCAGC 5 cut(s) 136, 658, 2027, 2759, 3389
BsgI GTGCAG 3 cut(s) 972, 1847, 2766
Bsh1236I CGCG 3 cut(s) 40, 131, 413
Bsh1285I CGRYCG 1 cut(s) 208
BshFI GGCC 7 cut(s) 103, 207, 1737, 2407, 3548, 3758, 3871
BshNI GGYRCC 2 cut(s) 34, 2671
BshTI ACCGGT 1 cut(s) 1158
BshVI ATCGAT 1 cut(s) 3876
BsiEI CGRYCG 1 cut(s) 208
BsiHKCI CYCGRG 1 cut(s) 468
BsiSI CCGG 6 cut(s) 70, 104, 1030, 1159, 2675, 3029
BslFI GGGAC 6 cut(s) 430, 2370, 2947, 2965, 3515, 3683
BslI CCNNNNNNNGG 9 cut(s) 371, 503, 1172, 1190, 1298, 2710, 3512, 3568, 3966
BsmAI GTCTC 7 cut(s) 152, 313, 471, 705, 2216, 2858, 3199
BsmBI CGTCTC 1 cut(s) 2858
BsmFI GGGAC 6 cut(s) 430, 2370, 2947, 2965, 3515, 3683
BsnI GGCC 7 cut(s) 103, 207, 1737, 2407, 3548, 3758, 3871
Bso31I GGTCTC 3 cut(s) 152, 471, 3199
BsoBI CYCGRG 1 cut(s) 468
Bsp120I GGGCCC 1 cut(s) 1735
Bsp1286I GDGCHC 2 cut(s) 1030, 1739
Bsp1407I TGTACA 4 cut(s) 580, 709, 1117, 1384
BspANI GGCC 7 cut(s) 103, 207, 1737, 2407, 3548, 3758, 3871
BspCNI CTCAG 7 cut(s) 61, 148, 328, 1442, 2755, 2870, 3220
BspDI ATCGAT 1 cut(s) 3876
BspFNI CGCG 3 cut(s) 40, 131, 413
BspHI TCATGA 1 cut(s) 1393
BspLI GGNNCC 5 cut(s) 36, 1426, 1737, 2673, 3549
BspMAI CTGCAG 2 cut(s) 676, 1084
BspMI ACCTGC 1 cut(s) 3968
BspOI GCTAGC 1 cut(s) 3395
BspPI GGATC 5 cut(s) 2360, 2549, 2936, 3411, 3642
BspT107I GGYRCC 2 cut(s) 34, 2671
BspTNI GGTCTC 3 cut(s) 152, 471, 3199
BsrBI CCGCTC 2 cut(s) 278, 1003
BsrDI GCAATG 3 cut(s) 159, 239, 3144
BsrFI RCCGGY 1 cut(s) 1158
BsrGI TGTACA 4 cut(s) 580, 709, 1117, 1384
BsrI ACTGG 7 cut(s) 591, 964, 1426, 2278, 2408, 3194, 3276
BssAI RCCGGY 1 cut(s) 1158
BssECI CCNNGG 7 cut(s) 98, 202, 292, 411, 2286, 2618, 3239
BssNI GRCGYC 1 cut(s) 114
BssT1I CCWWGG 3 cut(s) 98, 2618, 3239
Bst2UI CCWGG 5 cut(s) 294, 339, 762, 1446, 3397
Bst6I CTCTTC 2 cut(s) 24, 1815
BstACI GRCGYC 1 cut(s) 114
BstAPI GCANNNNNTGC 1 cut(s) 1025
BstAUI TGTACA 4 cut(s) 580, 709, 1117, 1384
BstBAI YACGTR 1 cut(s) 2744
BstC8I GCNNGC 9 cut(s) 129, 676, 823, 1213, 1849, 2088, 3393, 3709, 3995
BstDSI CCRYGG 1 cut(s) 411
BstEII GGTNACC 2 cut(s) 360, 1161
BstENI CCTNNNNNAGG 1 cut(s) 1296
BstFNI CGCG 3 cut(s) 40, 131, 413
BstMAI GTCTC 7 cut(s) 152, 313, 471, 705, 2216, 2858, 3199
BstMCI CGRYCG 1 cut(s) 208
BstMWI GCNNNNNNNGC 6 cut(s) 100, 857, 1025, 1034, 3500, 3983
BstNI CCWGG 5 cut(s) 294, 339, 762, 1446, 3397
BstNSI RCATGY 1 cut(s) 2090
BstPAI GACNNNNGTC 1 cut(s) 2998
BstPI GGTNACC 2 cut(s) 360, 1161
BstSCI CCNGG 7 cut(s) 292, 337, 760, 1028, 1444, 3028, 3395
BstSFI CTRYAG 5 cut(s) 672, 1080, 1434, 1769, 3019
BstSLI GKGCMC 2 cut(s) 1030, 1739
BstUI CGCG 3 cut(s) 40, 131, 413
BstV1I GCAGC 5 cut(s) 136, 658, 2027, 2759, 3389
BstV2I GAAGAC 2 cut(s) 1653, 3651
BstX2I RGATCY 4 cut(s) 1543, 2554, 3307, 3316
BstYI RGATCY 4 cut(s) 1543, 2554, 3307, 3316
BstZI CGGCCG 1 cut(s) 205
Bsu15I ATCGAT 1 cut(s) 3876
BsuI GTATCC 2 cut(s) 2243, 4048
BsuRI GGCC 7 cut(s) 103, 207, 1737, 2407, 3548, 3758, 3871
BsuTUI ATCGAT 1 cut(s) 3876
BtgI CCRYGG 1 cut(s) 411
BtrI CACGTC 1 cut(s) 1690
BtsI GCAGTG 1 cut(s) 2473
BtsIMutI CAGTG 4 cut(s) 957, 1419, 2450, 2473
BveI ACCTGC 1 cut(s) 3968
Cac8I GCNNGC 9 cut(s) 129, 676, 823, 1213, 1849, 2088, 3393, 3709, 3995
CciI TCATGA 1 cut(s) 1393
Cfr10I RCCGGY 1 cut(s) 1158
Cfr13I GGNCC 5 cut(s) 1425, 1735, 1736, 3134, 3547
Cfr42I CCGCGG 1 cut(s) 414
ClaI ATCGAT 1 cut(s) 3876
CseI GACGC 5 cut(s) 29, 116, 122, 902, 1689
Csp6I GTAC 9 cut(s) 581, 710, 1118, 1385, 2672, 3011, 3223, 3742, 4029
CspAI ACCGGT 1 cut(s) 1158
CspCI CAANNNNNGTGG 2 cut(s) 1921, 1956
CviQI GTAC 9 cut(s) 581, 710, 1118, 1385, 2672, 3011, 3223, 3742, 4029
DraI TTTAAA 1 cut(s) 2958
DrdI GACNNNNNNGTC 1 cut(s) 2030
DseDI GACNNNNNNGTC 1 cut(s) 2030
EaeI YGGCCR 2 cut(s) 101, 205
EagI CGGCCG 1 cut(s) 205
Eam1104I CTCTTC 2 cut(s) 24, 1815
EarI CTCTTC 2 cut(s) 24, 1815
EclXI CGGCCG 1 cut(s) 205
Eco130I CCWWGG 3 cut(s) 98, 2618, 3239
Eco24I GRGCYC 1 cut(s) 1739
Eco31I GGTCTC 3 cut(s) 152, 471, 3199
Eco32I GATATC 2 cut(s) 1916, 3026
Eco47I GGWCC 2 cut(s) 1425, 3134
Eco52I CGGCCG 1 cut(s) 205
Eco57I CTGAAG 4 cut(s) 185, 313, 3005, 3324
Eco72I CACGTG 1 cut(s) 2744
Eco88I CYCGRG 1 cut(s) 468
Eco91I GGTNACC 2 cut(s) 360, 1161
EcoNI CCTNNNNNAGG 1 cut(s) 1296
EcoO109I RGGNCCY 3 cut(s) 1425, 1735, 3547
EcoO65I GGTNACC 2 cut(s) 360, 1161
EcoRI GAATTC 1 cut(s) 1490
EcoRII CCWGG 5 cut(s) 292, 337, 760, 1444, 3395
EcoRV GATATC 2 cut(s) 1916, 3026
EcoT14I CCWWGG 3 cut(s) 98, 2618, 3239
EcoT22I ATGCAT 4 cut(s) 613, 1637, 2088, 2664
EcoT38I GRGCYC 1 cut(s) 1739
ErhI CCWWGG 3 cut(s) 98, 2618, 3239
Esp3I CGTCTC 1 cut(s) 2858
FaqI GGGAC 6 cut(s) 430, 2370, 2947, 2965, 3515, 3683
FauI CCCGC 1 cut(s) 406
FauNDI CATATG 1 cut(s) 1312
FbaI TGATCA 1 cut(s) 1282
FblI GTMKAC 4 cut(s) 43, 980, 1109, 3018
FriOI GRGCYC 1 cut(s) 1739
GsuI CTGGAG 3 cut(s) 321, 1467, 1545
HaeIII GGCC 7 cut(s) 103, 207, 1737, 2407, 3548, 3758, 3871
HapII CCGG 6 cut(s) 70, 104, 1030, 1159, 2675, 3029
HgaI GACGC 5 cut(s) 29, 116, 122, 902, 1689
Hin1I GRCGYC 1 cut(s) 114
HincII GTYRAC 1 cut(s) 1708
HindII GTYRAC 1 cut(s) 1708
HindIII AAGCTT 2 cut(s) 2706, 3249
HpaII CCGG 6 cut(s) 70, 104, 1030, 1159, 2675, 3029
Hpy99I CGWCG 2 cut(s) 256, 1656
HpyCH4IV ACGT 9 cut(s) 282, 725, 1689, 1704, 1710, 2483, 2743, 3139, 3416
HpyF10VI GCNNNNNNNGC 6 cut(s) 100, 857, 1025, 1034, 3500, 3983
HpySE526I ACGT 9 cut(s) 282, 725, 1689, 1704, 1710, 2483, 2743, 3139, 3416
Hsp92I GRCGYC 1 cut(s) 114
KpnI GGTACC 1 cut(s) 2675
Ksp22I TGATCA 1 cut(s) 1282
KspI CCGCGG 1 cut(s) 414
LmnI GCTCC 6 cut(s) 72, 580, 865, 1243, 1770, 2508
Lsp1109I GCAGC 5 cut(s) 136, 658, 2027, 2759, 3389
LweI GCATC 8 cut(s) 198, 598, 1644, 2002, 2707, 3490, 3694, 3763
MaeII ACGT 9 cut(s) 282, 725, 1689, 1704, 1710, 2483, 2743, 3139, 3416
MbiI CCGCTC 2 cut(s) 278, 1003
MfeI CAATTG 1 cut(s) 1046
MflI RGATCY 4 cut(s) 1543, 2554, 3307, 3316
MhlI GDGCHC 2 cut(s) 1030, 1739
MlyI GAGTC 1 cut(s) 3292
MmeI TCCRAC 5 cut(s) 613, 1021, 2582, 2969, 3076
Mph1103I ATGCAT 4 cut(s) 613, 1637, 2088, 2664
MroXI GAANNNNTTC 3 cut(s) 1401, 1893, 3318
MslI CAYNNNNRTG 2 cut(s) 2241, 2265
MspA1I CMGCKG 5 cut(s) 413, 490, 1037, 2939, 3380
MspI CCGG 6 cut(s) 70, 104, 1030, 1159, 2675, 3029
MspR9I CCNGG 7 cut(s) 294, 339, 762, 1030, 1446, 3030, 3397
MunI CAATTG 1 cut(s) 1046
MvaI CCWGG 5 cut(s) 294, 339, 762, 1446, 3397
MvnI CGCG 3 cut(s) 40, 131, 413
MwoI GCNNNNNNNGC 6 cut(s) 100, 857, 1025, 1034, 3500, 3983
NciI CCSGG 2 cut(s) 1030, 3030
NdeI CATATG 1 cut(s) 1312
NheI GCTAGC 1 cut(s) 3391
NlaIV GGNNCC 5 cut(s) 36, 1426, 1737, 2673, 3549
NmeAIII GCCGAG 1 cut(s) 183
NmuCI GTSAC 9 cut(s) 353, 360, 492, 890, 909, 1084, 1161, 2002, 3428
NsiI ATGCAT 4 cut(s) 613, 1637, 2088, 2664
NspI RCATGY 1 cut(s) 2090
PaeI GCATGC 1 cut(s) 2090
PaeR7I CTCGAG 1 cut(s) 468
PagI TCATGA 1 cut(s) 1393
PcsI WCGNNNNNNNCGW 1 cut(s) 2633
PdmI GAANNNNTTC 3 cut(s) 1401, 1893, 3318
PfeI GAWTC 9 cut(s) 1673, 1894, 2219, 2277, 2524, 2834, 2915, 3086, 3832
PflMI CCANNNNNTGG 2 cut(s) 2710, 3568
PinAI ACCGGT 1 cut(s) 1158
PleI GAGTC 1 cut(s) 3291
PmaCI CACGTG 1 cut(s) 2744
PmlI CACGTG 1 cut(s) 2744
PpsI GAGTC 1 cut(s) 3291
Ppu21I YACGTR 1 cut(s) 2744
PpuMI RGGWCCY 1 cut(s) 1425
PshAI GACNNNNGTC 1 cut(s) 2998
PshBI ATTAAT 1 cut(s) 3611
PsiI TTATAA 1 cut(s) 3618
Psp1406I AACGTT 1 cut(s) 2483
Psp5II RGGWCCY 1 cut(s) 1425
Psp6I CCWGG 5 cut(s) 292, 337, 760, 1444, 3395
PspCI CACGTG 1 cut(s) 2744
PspEI GGTNACC 2 cut(s) 360, 1161
PspGI CCWGG 5 cut(s) 292, 337, 760, 1444, 3395
PspN4I GGNNCC 5 cut(s) 36, 1426, 1737, 2673, 3549
PspOMI GGGCCC 1 cut(s) 1735
PspPI GGNCC 5 cut(s) 1425, 1735, 1736, 3134, 3547
PspPPI RGGWCCY 1 cut(s) 1425
PsrI GAACNNNNNNTAC 2 cut(s) 3569, 3601
PstI CTGCAG 2 cut(s) 676, 1084
PsuI RGATCY 4 cut(s) 1543, 2554, 3307, 3316
PvuII CAGCTG 4 cut(s) 490, 1037, 2939, 3380
RsaI GTAC 9 cut(s) 582, 711, 1119, 1386, 2673, 3012, 3224, 3743, 4030
RsaNI GTAC 9 cut(s) 581, 710, 1118, 1385, 2672, 3011, 3223, 3742, 4029
RseI CAYNNNNRTG 2 cut(s) 2241, 2265
SacII CCGCGG 1 cut(s) 414
Sau96I GGNCC 5 cut(s) 1425, 1735, 1736, 3134, 3547
SchI GAGTC 1 cut(s) 3292
ScrFI CCNGG 7 cut(s) 294, 339, 762, 1030, 1446, 3030, 3397
SduI GDGCHC 2 cut(s) 1030, 1739
SfaNI GCATC 8 cut(s) 198, 598, 1644, 2002, 2707, 3490, 3694, 3763
SfcI CTRYAG 5 cut(s) 672, 1080, 1434, 1769, 3019
Sfr274I CTCGAG 1 cut(s) 468
Sfr303I CCGCGG 1 cut(s) 414
SgrBI CCGCGG 1 cut(s) 414
SinI GGWCC 2 cut(s) 1425, 3134
SlaI CTCGAG 1 cut(s) 468
SmiMI CAYNNNNRTG 2 cut(s) 2241, 2265
SmlI CTYRAG 3 cut(s) 468, 2378, 3196
SmoI CTYRAG 3 cut(s) 468, 2378, 3196
SpeI ACTAGT 1 cut(s) 3013
SphI GCATGC 1 cut(s) 2090
SspI AATATT 1 cut(s) 1580
StyD4I CCNGG 7 cut(s) 292, 337, 760, 1028, 1444, 3028, 3395
StyI CCWWGG 3 cut(s) 98, 2618, 3239
TaiI ACGT 9 cut(s) 285, 728, 1692, 1707, 1713, 2486, 2746, 3142, 3419
TatI WGTACW 5 cut(s) 580, 709, 1117, 1384, 3741
TauI GCSGC 8 cut(s) 40, 134, 152, 413, 545, 1211, 1556, 1912
TfiI GAWTC 9 cut(s) 1673, 1894, 2219, 2277, 2524, 2834, 2915, 3086, 3832
TscAI CASTG 4 cut(s) 964, 1426, 2450, 2473
TseFI GTSAC 9 cut(s) 353, 360, 492, 890, 909, 1084, 1161, 2002, 3428
TseI GCWGC 5 cut(s) 124, 671, 2015, 2747, 3377
Tsp45I GTSAC 9 cut(s) 353, 360, 492, 890, 909, 1084, 1161, 2002, 3428
TspGWI ACGGA 2 cut(s) 240, 1484
TspRI CASTG 4 cut(s) 964, 1426, 2450, 2473
Van91I CCANNNNNTGG 2 cut(s) 2710, 3568
VpaK11BI GGWCC 2 cut(s) 1425, 3134
VspI ATTAAT 1 cut(s) 3611
XagI CCTNNNNNAGG 1 cut(s) 1296
XapI RAATTY 4 cut(s) 1490, 1608, 1660, 2681
XbaI TCTAGA 4 cut(s) 751, 2827, 3310, 3496
XceI RCATGY 1 cut(s) 2090
XcmI CCANNNNNNNNNTGG 1 cut(s) 3237
XhoI CTCGAG 1 cut(s) 468
XmaJI CCTAGG 1 cut(s) 2618
XmiI GTMKAC 4 cut(s) 43, 980, 1109, 3018
XmnI GAANNNNTTC 3 cut(s) 1401, 1893, 3318
Zsp2I ATGCAT 4 cut(s) 613, 1637, 2088, 2664
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.