Rh2CG065600
ERF Family

Probably involved in the RNA silencing pathway and required for the generation of small interfering RNAs (siRNAs)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0000505
Physical Location & Seq
Forward (+)
5453987 .. 5464874
10888 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG065600.1

Sequence Viewer

Length: 4068 bp
ATGATGAAGCAGAGTTGGGATGAGCAAGAAGAGGTGCCGCGTCTGCCTCAGAAAGTGGAGTTATTGCTCCGGCAAATCTGTGAGGACAAAAAGCAACCTTGGCCGGACGCCGACCCCATAAGGCAGCTCGCGACTCAGAGCGAGGAGTCGGCAATGGAGACCCTGAAGAAAATCTCCTCCACCAGCAAAAGCATCAGAACCCTCGGCGGTTATATAAAGTGGATGATCGGGCACTGTAGCTCTGCTTCTCCGTCGCCGTCCAAAACGGTGCGTTCCGCTCACGTTCAAAGCCCTGGTGCTACTTCACTCACTCCTCAGAGACTTCACTTCAGCCCTCCAGGTGAGGCAGTTAGTGACAGGTCACCTGTTGTTGGGCAATTACCTTATTGCTCTGGTTCTCCATCGAAAGCCGCGGGGACTTGTTCTTGTCAAAGATTGTCTGCTCATGCACAAGTTGATGAGAGGTCTCGAGGCGAGGCATTTAATCAGCTGTCACCTGTTATTGGTCAATCACCTTATTCCTCTGGTTCTCCTTTGAAAGCCGCAGACACTTCTTCTTATCAAAGACCGTCTGCTCCTGTACAACTGGATGAGAGATTTCAAGGTGATGTATTTAGTCATCGGTCATCTGTTGGACAACCACCTTATTCCTCTGCTTCTCCATCAAAAGCAGCAGGCACTACTTCTTATCAAAGATTGTCTCCTCCTGTACAACTTGATGATACGTTTCAAGGTGAGGCATTTACTGTTCTAGAGCAACCAGGAAACATCAATAATGGACAATCACCTTATTATTCCTCTGGTTCTCCATTGAAAGTGGCACGCATTTGTTCTTGTCAAAGCCCATCTGCGTTGGTTGCTCCTATACAACTTGATAAGAAGTTTCAAGGTGACCCATTTACTGACAGGTCACTTGTTCAACAATTACCTTGTTCCTCTGGTTCTCCATTAAAAGCCACAGGTTCTTATGAAAGATTGTCTACTCCTGCCCAAGCTGATGAGCGGTTTCCAGGTGAGGCATTTAGTGCCCAGTCAGCTGTTGGACAATTACCTTGTTCCTCTGTTTCTCCATTAAAATCCGCAGTCAGTTCTTCTTATCAAAGATTGTCTACTCCTGTACAGCTTGATGAGAGATTTCAAGTTGAGGCATTTAATGACCGGTCACCTGTTATTGGACAATCACCTTATTCCTCTGGTTCTCCGTTGAAAGCCGCAAGCACTTCTTATCAAAGACTGACTGCTTCTCTACAACTTGATGAGAGGTTTCAAGGTGGGGTATTTGCTGATCAGACACCTGTTCAAGGACAATCACCATTTGCCTCTTGTTCTGCATCAAAAGCCGTCTGCATTTCTCCTTATCAGAGAATGTCTGCTGATGTTGTTCCTGTACAACTTCATGAAAGGTTTCAAGGTGAGGGTATCACTAGGTCCTCAGTTATAGACAAACCTGGAGATCATGGAGAAGCACACTTAGTAGCTCTGGGTGAACTAGAATTCCGTAAACAATTTCTGATTTTAAGTTATGCTGGAGGAACGAAGTTGAGAGATCTTATAGCGGCAGAGGACATTAGGCATTGGAAATATTTGCCCATGGTAACGTTTGAGAGCAAAATTTGGGAGTATCTAGGTCGAAAATGCATCAGCCTCGAAGACCGTCGATTGAATTTTGATTGGGATTCTGGACGCACACACGTCTATCATTGTTACGTCAATGTGGATGGGAGTTACAGATTTAAGGGCCCATATTTATATAAAATAAGAAGAACGCTCCTACAGAAAAAATTAGGAGATGACAATGTTTTAATGGTGAAGTTTGCCGAAGAAGAGGTGCAGAGAGGTCAAAGTATTGCTTGCCATCATAATTATTCTATGTATATGAAGGTTGCAAGAGAAGGAATTCTTGTTGGTTTGCGGCGATATCGTTTCTTTGTGTTCAAAGATGGTGGTAATGAGGAGAAGAAAAAAAACCCAACTTCCTCACCTGTGAAGTGTTACTTTGTTCGAGTGGCCTCTGATGCAGCCATAGACAAAGATGTCAGTTATATATTATCTAACAGAACGATGTATGAAGCCAGATGTCTTTTCATGCATGCACATACTCTGGCTAGTATTGATAACTCCATGGCTAGGTTCTCACTCATTTTGTCCAAGACAACGAGCTTAAAAGTAGATTGGTCAGTTGTCAAAGTTGAAATCATCGATGAAGAATACTGTCACGATGAATCTGGTAATCGTATCCATAGAGATGAAAAACCACTTATACATACAGATGGAACTGGATTCATATCCGAGGATTTGGCTTTAATTTGTCCAAAGATAGAAGCCAAAGGACAATGTATAAGTGATGAACACATGAAGGGACTTCCTGATCCTGGTGAACTTGAGGATAACGATATGGGAAAGAAAAGGCCAGTTCTTAGAACTGAAGAACCGCCTTTGCTCACACAGTGCCGACTTTTTCACAGTGGCAGTGCTTCTAAAGGAACGTTTCTTGTCAATAAAAAGCTCCATCCGAACACAATCCAGATTCGACCATCTATGCTCAAAGTTAAGACAGATCCAATGATTTCCAATAATCAAACAATAAACTCATTGGAAATAGTTGGCACAAGTACTCCTCCTAGGAATACGCACTTATCGAGGATTTTAATTGCACTTCTAATGCATGGAGGGGTACCAGAAGAATTTTTCATGCAGATACTTAACGAAGCTTTGGAAGATGCTCATGGTGTTTTCTGCAAGACACGTGCAGCACTCAAAGTTGCTGAGAAGTATGGTGAGATCGATGACAATTTCTTGGGGAAATTAATGATCTCATTTGGAATACCTCTAGAAGAATCATACTTGCAGTATCGTCTCTCAGTCCTAATGAAAGAGGAAAACAACAGTCTTAGAAAAGGGAAACTTTATATTCCTGATTCATATAATTTGATGGGGACAGCTGATCCAACAGGGACTTTAAAGAAGGATGAAGTCTGTGTTATCCTGAAGGATGGACAACTGTCAGGGAAGGTACTAGTGTACAGATATCCGGGTTTACACTTTGGGGATATTCATGTTCTAAAGGCGACCTATGTGAAGGAGTTAGAGTCTTTTGTTGGAAATGCCAAATACGGTATATTCTTCTCTTGCAAAGGTCCACGTTCCATTGCTGATGAAATGGGTGGTGGAGATTTTGATGGTGATCTATACTGGGTCTCAAGAAATCCTCAGCTATTGGAGTGGTACAAACCAAGTGAACCTTGGATTGAAGCTTCAATATCAACCCAAAAAGTTCCCAGTAGGAGTCCATCTGACTATTCGCCTGAAGATCTAGAAGATCTTCTGTTTAGATCATTCTTGACAACTAGGTTTGAACCAAGTTTCGCTGCGAGTGAGGCAGCTGCTAATTGGCTAGCCTGGATGGATCGATTCTTGACTTTGGGAGAGAGTGACATTGACGAAAAGAATAATACGAAGGAGAAGATGCTGCAACTAATTGACTTATACTACACAGCTCTAGATGCACCAAAGAAAGGTGTAAAGGTTGTGGTCCCAAAAGAATTGAAGGTCCCATGTTTCCCTCATTACATGGGAAAGAACAATTCTTACCCTTCAACTTCCATTTTGGGATTAATTTATGACAGGGTAGATGAGCATCAAACAGAAGACCGTTCTCTCAAAGAAGTCGAGAAACTTCCTGCTTTTGATGTTAATGAAGTCCCTGATGCCTGCTTCGACAAATGGGAGGAACTACACCGACAGTACAGGAGTGAGATGGCCGCTGCTATGGACGATACTGATAAACAGAGCAGAAATCAGTCTGCTGATGTGGTCATTAGGAAATATAAAAAGATTCTGTACGAGGCTAATGACTTTGAGGAGAGTAGAAGGCCGATCGATGAGATATATAACGAGGCACAAGCTATATATCAATTGTGTTATGACCATGCGAGGAGGGTAGGAAGTGTGAAGGCGTGTTCCTTTGCATGGAGAGTTGCAGGTAAAGCTCTGTGCCAGCTTCACGCCAAGAACACGAATGAAAGGTGTTGTACAATAGTATCCTCTGTTTTGAAGGAGATTTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RdRP

Protein Analysis

1355

Amino Acids

151.53

Weight (kDa)

6.71

Isoelectric Point (pI)

49.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RdRP_helical PF26252 490 - 554 2.1e-07 RNA dependent RNA polymerase, helical domain
RdRP PF05183 577 - 1210 1.4e-100 RNA dependent RNA polymerase
RdRP_head PF26253 1233 - 1339 6.3e-13 RdRP, head domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000505)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G19910 AT2G19910 AT2G19920 AT2G19920 AT2G19920 AT2G19920 AT2G19930 AT2G19930 AT2G19930
fragaria_vesca FvH4_1g05980 FvH4_1g05990 FvH4_1g05990 FvH4_1g09718
malus_domestica MD02G1064400.v1.1 MD15G1195300.v1.1 MD15G1195400.v1.1
prunus_persica Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1
pyrus_communis pycom02g05070 pycom15g17460
rosa_chinensis RchiOBHm_Chr2g0091671 RchiOBHm_Chr2g0091681 RchiOBHm_Chr2g0091691 RchiOBHm_Chr2g0091701 RchiOBHm_Chr2g0091711 RchiOBHm_Chr2g0091721 RchiOBHm_Chr2g0091731 RchiOBHm_Chr5g0014401
rosa_laevigata RLG00000013772 RLG00000016242 RLG00000016244 RLG00000016245 RLG00000016246 RLG00000032105
rosa_multiflora Rmu_co8374041.1_g000001 Rmu_co8478121.1_g000001 Rmu_sc0000897.1_g000005 Rmu_sc0000897.1_g000009
rosa_roxburghii Rroxscaffold_1G00062180 Rroxscaffold_1G00062190 Rroxscaffold_1G00068800 Rroxscaffold_2G00149790 Rroxscaffold_2G00149800 Rroxscaffold_2G00149810
rosa_rugosa Rorug02G0019600 Rorug02G0019700 Rorug02G0019800 Rorug02G0019900 Rorug02G0019900 Rorug02G0020000 Rorug02G0020000 Rorug02G0020100 Rorug02G0020100 Rorug02G0020200 Rorug05G0017800
rosa_samantha Rh2AG064700 Rh2AG064800 Rh2AG064900 Rh2BG063800 Rh2BG063900 Rh2BG064000 Rh2BG064100 Rh2CG065500 Rh2CG065600 Rh2CG065700 Rh2DG063900 Rh2DG064000 Rh2DG064100 Rh2DG064200 Rh2DG381700 Rh5AG056700 Rh5AG056800 Rh5AG056900 Rh5AG112600 Rh5BG109300 Rh5CG063800 Rh5CG121100 Rh5DG053100 Rh5DG108300 Rh6CG100100
rosa_wichuraiana Rw2G005560 Rw2G005580 Rw2G005590 Rw5G005370 Rw5G009770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2033
Acc36I ACCTGC 1 cut(s) 3971
Acc65I GGTACC 1 cut(s) 2674
AccB1I GGYRCC 2 cut(s) 34, 2674
AccBSI CCGCTC 2 cut(s) 278, 1003
AccI GTMKAC 2 cut(s) 980, 1109
AccII CGCG 3 cut(s) 40, 131, 413
AclI AACGTT 2 cut(s) 1598, 2486
AclWI GGATC 4 cut(s) 2363, 2552, 2939, 3414
AcoI YGGCCR 2 cut(s) 101, 3759
AcsI RAATTY 5 cut(s) 1493, 1611, 1663, 1896, 2684
AcuI CTGAAG 5 cut(s) 185, 313, 2445, 3008, 3327
AcvI CACGTG 1 cut(s) 2747
AcyI GRCGYC 1 cut(s) 108
AdeI CACNNNGTG 1 cut(s) 2448
AfiI CCNNNNNNNGG 8 cut(s) 371, 503, 1172, 1301, 2127, 2372, 3515, 3969
AflIII ACRYGT 2 cut(s) 1690, 2744
AgeI ACCGGT 1 cut(s) 1158
AhlI ACTAGT 1 cut(s) 3016
AjiI CACGTC 1 cut(s) 1693
AjnI CCWGG 7 cut(s) 292, 337, 760, 1009, 1447, 2371, 3398
AloI GAACNNNNNNTCC 2 cut(s) 1479, 1511
Alw26I GTCTC 6 cut(s) 152, 313, 471, 705, 2861, 3202
AlwI GGATC 4 cut(s) 2363, 2552, 2939, 3414
Ama87I CYCGRG 1 cut(s) 468
AoxI GGCC 6 cut(s) 101, 1738, 2007, 2408, 3759, 3872
ApaI GGGCCC 1 cut(s) 1742
ApeKI GCWGC 9 cut(s) 124, 671, 2018, 2750, 3368, 3380, 3383, 3469, 3764
ApoI RAATTY 5 cut(s) 1493, 1611, 1663, 1896, 2684
AseI ATTAAT 2 cut(s) 2807, 3614
AsiGI ACCGGT 1 cut(s) 1158
Asp700I GAANNNNTTC 3 cut(s) 1404, 1896, 3321
Asp718I GGTACC 1 cut(s) 2674
AspA2I CCTAGG 1 cut(s) 2621
AspS9I GGNCC 6 cut(s) 1428, 1738, 1739, 3137, 3532, 3550
AsuC2I CCSGG 1 cut(s) 3033
AsuNHI GCTAGC 1 cut(s) 3394
AvaI CYCGRG 1 cut(s) 468
AvaII GGWCC 4 cut(s) 1428, 3137, 3532, 3550
AvrII CCTAGG 1 cut(s) 2621
BaeGI GKGCMC 3 cut(s) 234, 1030, 1742
BaeI ACNNNNGTAYC 4 cut(s) 3005, 3038, 4023, 4056
BanI GGYRCC 2 cut(s) 34, 2674
BanII GRGCYC 1 cut(s) 1742
BarI GAAGNNNNNNTAC 2 cut(s) 229, 261
BbrPI CACGTG 1 cut(s) 2747
BbsI GAAGAC 2 cut(s) 1656, 3654
BbvCI CCTCAGC 1 cut(s) 3210
BbvI GCAGC 9 cut(s) 136, 683, 2030, 2762, 3355, 3370, 3392, 3456, 3751
BceAI ACGGC 2 cut(s) 241, 1325
BcgI CGANNNNNNTGC 2 cut(s) 1627, 1661
BciT130I CCWGG 7 cut(s) 294, 339, 762, 1011, 1449, 2373, 3400
BciVI GTATCC 2 cut(s) 2246, 4051
BclI TGATCA 1 cut(s) 1285
BcnI CCSGG 1 cut(s) 3033
BcoDI GTCTC 6 cut(s) 152, 313, 471, 705, 2861, 3202
BcuI ACTAGT 1 cut(s) 3016
BfmI CTRYAG 2 cut(s) 235, 1772
BfuAI ACCTGC 1 cut(s) 3971
BfuI GTATCC 2 cut(s) 2246, 4051
BglII AGATCT 3 cut(s) 1546, 3310, 3319
BlnI CCTAGG 1 cut(s) 2621
BmcAI AGTACT 1 cut(s) 2614
Bme1390I CCNGG 8 cut(s) 294, 339, 762, 1011, 1449, 2373, 3033, 3400
Bme18I GGWCC 4 cut(s) 1428, 3137, 3532, 3550
BmeT110I CYCGRG 1 cut(s) 468
BmgBI CACGTC 1 cut(s) 1693
BmgT120I GGNCC 6 cut(s) 1428, 1738, 1739, 3137, 3532, 3550
BmiI GGNNCC 5 cut(s) 36, 1740, 2676, 3534, 3552
BmrFI CCNGG 8 cut(s) 294, 339, 762, 1011, 1449, 2373, 3033, 3400
BmrI ACTGGG 3 cut(s) 1024, 3202, 3273
BmsI GCATC 9 cut(s) 201, 1340, 1647, 2005, 2710, 3456, 3493, 3646, 3697
BmtI GCTAGC 1 cut(s) 3398
BmuI ACTGGG 3 cut(s) 1024, 3202, 3273
BoxI GACNNNNGTC 1 cut(s) 3001
BpiI GAAGAC 2 cut(s) 1656, 3654
BpmI CTGGAG 3 cut(s) 321, 1470, 1548
Bpu10I CCTNAGC 1 cut(s) 3210
BpuEI CTTGAG 2 cut(s) 2402, 3184
BpuMI CCSGG 1 cut(s) 3033
Bsa29I ATCGAT 4 cut(s) 2199, 2784, 3409, 3879
BsaAI YACGTR 1 cut(s) 2747
BsaBI GATNNNNATC 3 cut(s) 2284, 3183, 3636
BsaHI GRCGYC 1 cut(s) 108
BsaI GGTCTC 3 cut(s) 152, 471, 3202
BsaJI CCNNGG 9 cut(s) 98, 202, 292, 411, 1590, 2121, 2289, 2621, 3242
BsaWI WCCGGW 1 cut(s) 1158
BsaXI ACNNNNNCTCC 5 cut(s) 2599, 2629, 3074, 3104, 4049
Bsc4I CCNNNNNNNGG 8 cut(s) 371, 503, 1172, 1301, 2127, 2372, 3515, 3969
Bse118I RCCGGY 1 cut(s) 1158
Bse1I ACTGG 6 cut(s) 591, 1030, 2281, 2411, 3197, 3279
Bse3DI GCAATG 2 cut(s) 159, 3147
Bse8I GATNNNNATC 3 cut(s) 2284, 3183, 3636
BseBI CCWGG 7 cut(s) 294, 339, 762, 1011, 1449, 2373, 3400
BseCI ATCGAT 4 cut(s) 2199, 2784, 3409, 3879
BseDI CCNNGG 9 cut(s) 98, 202, 292, 411, 1590, 2121, 2289, 2621, 3242
BseGI GGATG 8 cut(s) 25, 228, 595, 1723, 2509, 2974, 2998, 3408
BseJI GATNNNNATC 3 cut(s) 2284, 3183, 3636
BseLI CCNNNNNNNGG 8 cut(s) 371, 503, 1172, 1301, 2127, 2372, 3515, 3969
BseMI GCAATG 2 cut(s) 159, 3147
BseMII CTCAG 7 cut(s) 62, 149, 329, 1446, 2757, 2874, 3224
BseNI ACTGG 6 cut(s) 591, 1030, 2281, 2411, 3197, 3279
BseRI GAGGAG 8 cut(s) 158, 166, 303, 693, 1967, 2607, 3875, 3949
BseSI GKGCMC 3 cut(s) 234, 1030, 1742
BseXI GCAGC 9 cut(s) 136, 683, 2030, 2762, 3355, 3370, 3392, 3456, 3751
BsgI GTGCAG 2 cut(s) 1850, 2769
Bsh1236I CGCG 3 cut(s) 40, 131, 413
Bsh1285I CGRYCG 1 cut(s) 3879
BshFI GGCC 6 cut(s) 103, 1740, 2009, 2410, 3761, 3874
BshNI GGYRCC 2 cut(s) 34, 2674
BshTI ACCGGT 1 cut(s) 1158
BshVI ATCGAT 4 cut(s) 2199, 2784, 3409, 3879
BsiEI CGRYCG 1 cut(s) 3879
BsiHKCI CYCGRG 1 cut(s) 468
BsiSI CCGG 4 cut(s) 70, 104, 1159, 3032
BslFI GGGAC 7 cut(s) 430, 2373, 2950, 2968, 3518, 3536, 3686
BslI CCNNNNNNNGG 8 cut(s) 371, 503, 1172, 1301, 2127, 2372, 3515, 3969
BsmAI GTCTC 6 cut(s) 152, 313, 471, 705, 2861, 3202
BsmBI CGTCTC 1 cut(s) 2861
BsmFI GGGAC 7 cut(s) 430, 2373, 2950, 2968, 3518, 3536, 3686
BsnI GGCC 6 cut(s) 103, 1740, 2009, 2410, 3761, 3874
Bso31I GGTCTC 3 cut(s) 152, 471, 3202
BsoBI CYCGRG 1 cut(s) 468
Bsp120I GGGCCC 1 cut(s) 1738
Bsp1286I GDGCHC 3 cut(s) 234, 1030, 1742
Bsp1407I TGTACA 6 cut(s) 580, 709, 1117, 1387, 3021, 4031
Bsp19I CCATGG 2 cut(s) 1590, 2121
Bsp68I TCGCGA 1 cut(s) 131
BspANI GGCC 6 cut(s) 103, 1740, 2009, 2410, 3761, 3874
BspCNI CTCAG 7 cut(s) 61, 148, 328, 1445, 2758, 2873, 3223
BspDI ATCGAT 4 cut(s) 2199, 2784, 3409, 3879
BspFNI CGCG 3 cut(s) 40, 131, 413
BspHI TCATGA 1 cut(s) 1396
BspLI GGNNCC 5 cut(s) 36, 1740, 2676, 3534, 3552
BspMI ACCTGC 1 cut(s) 3971
BspOI GCTAGC 1 cut(s) 3398
BspPI GGATC 4 cut(s) 2363, 2552, 2939, 3414
BspT107I GGYRCC 2 cut(s) 34, 2674
BspTNI GGTCTC 3 cut(s) 152, 471, 3202
BsrBI CCGCTC 2 cut(s) 278, 1003
BsrDI GCAATG 2 cut(s) 159, 3147
BsrFI RCCGGY 1 cut(s) 1158
BsrGI TGTACA 6 cut(s) 580, 709, 1117, 1387, 3021, 4031
BsrI ACTGG 6 cut(s) 591, 1030, 2281, 2411, 3197, 3279
BssAI RCCGGY 1 cut(s) 1158
BssECI CCNNGG 9 cut(s) 98, 202, 292, 411, 1590, 2121, 2289, 2621, 3242
BssNI GRCGYC 1 cut(s) 108
BssT1I CCWWGG 5 cut(s) 98, 1590, 2121, 2621, 3242
Bst2UI CCWGG 7 cut(s) 294, 339, 762, 1011, 1449, 2373, 3400
Bst6I CTCTTC 2 cut(s) 24, 1818
BstACI GRCGYC 1 cut(s) 108
BstAPI GCANNNNNTGC 1 cut(s) 1025
BstAUI TGTACA 6 cut(s) 580, 709, 1117, 1387, 3021, 4031
BstBAI YACGTR 1 cut(s) 2747
BstC8I GCNNGC 9 cut(s) 129, 676, 823, 1216, 1852, 2091, 3396, 3712, 3998
BstDSI CCRYGG 3 cut(s) 411, 1590, 2121
BstEII GGTNACC 3 cut(s) 360, 890, 1161
BstENI CCTNNNNNAGG 1 cut(s) 1299
BstF5I GGATG 8 cut(s) 25, 228, 595, 1723, 2509, 2974, 2998, 3408
BstFNI CGCG 3 cut(s) 40, 131, 413
BstMAI GTCTC 6 cut(s) 152, 313, 471, 705, 2861, 3202
BstMCI CGRYCG 1 cut(s) 3879
BstNI CCWGG 7 cut(s) 294, 339, 762, 1011, 1449, 2373, 3400
BstNSI RCATGY 1 cut(s) 2093
BstPAI GACNNNNGTC 1 cut(s) 3001
BstPI GGTNACC 3 cut(s) 360, 890, 1161
BstSCI CCNGG 8 cut(s) 292, 337, 760, 1009, 1447, 2371, 3031, 3398
BstSFI CTRYAG 2 cut(s) 235, 1772
BstSLI GKGCMC 3 cut(s) 234, 1030, 1742
BstUI CGCG 3 cut(s) 40, 131, 413
BstV1I GCAGC 9 cut(s) 136, 683, 2030, 2762, 3355, 3370, 3392, 3456, 3751
BstV2I GAAGAC 2 cut(s) 1656, 3654
BstX2I RGATCY 4 cut(s) 1546, 2557, 3310, 3319
BstYI RGATCY 4 cut(s) 1546, 2557, 3310, 3319
Bsu15I ATCGAT 4 cut(s) 2199, 2784, 3409, 3879
BsuI GTATCC 2 cut(s) 2246, 4051
BsuRI GGCC 6 cut(s) 103, 1740, 2009, 2410, 3761, 3874
BsuTUI ATCGAT 4 cut(s) 2199, 2784, 3409, 3879
BtgI CCRYGG 3 cut(s) 411, 1590, 2121
BtrI CACGTC 1 cut(s) 1693
BtsCI GGATG 8 cut(s) 25, 228, 595, 1723, 2509, 2974, 2998, 3408
BtsI GCAGTG 1 cut(s) 2476
BtsIMutI CAGTG 4 cut(s) 232, 2453, 2470, 2476
BtuMI TCGCGA 1 cut(s) 131
BveI ACCTGC 1 cut(s) 3971
Cac8I GCNNGC 9 cut(s) 129, 676, 823, 1216, 1852, 2091, 3396, 3712, 3998
CciI TCATGA 1 cut(s) 1396
Cfr10I RCCGGY 1 cut(s) 1158
Cfr13I GGNCC 6 cut(s) 1428, 1738, 1739, 3137, 3532, 3550
Cfr42I CCGCGG 1 cut(s) 414
ClaI ATCGAT 4 cut(s) 2199, 2784, 3409, 3879
CseI GACGC 3 cut(s) 29, 116, 1692
CspAI ACCGGT 1 cut(s) 1158
CspCI CAANNNNNGTGG 2 cut(s) 1924, 1959
DraI TTTAAA 1 cut(s) 2961
DraIII CACNNNGTG 1 cut(s) 2448
DrdI GACNNNNNNGTC 1 cut(s) 2033
DseDI GACNNNNNNGTC 1 cut(s) 2033
EaeI YGGCCR 2 cut(s) 101, 3759
Eam1104I CTCTTC 2 cut(s) 24, 1818
EarI CTCTTC 2 cut(s) 24, 1818
Eco130I CCWWGG 5 cut(s) 98, 1590, 2121, 2621, 3242
Eco24I GRGCYC 1 cut(s) 1742
Eco31I GGTCTC 3 cut(s) 152, 471, 3202
Eco32I GATATC 2 cut(s) 1919, 3029
Eco47I GGWCC 4 cut(s) 1428, 3137, 3532, 3550
Eco57I CTGAAG 5 cut(s) 185, 313, 2445, 3008, 3327
Eco72I CACGTG 1 cut(s) 2747
Eco88I CYCGRG 1 cut(s) 468
Eco91I GGTNACC 3 cut(s) 360, 890, 1161
EcoNI CCTNNNNNAGG 1 cut(s) 1299
EcoO109I RGGNCCY 3 cut(s) 1428, 1738, 3550
EcoO65I GGTNACC 3 cut(s) 360, 890, 1161
EcoRI GAATTC 2 cut(s) 1493, 1896
EcoRII CCWGG 7 cut(s) 292, 337, 760, 1009, 1447, 2371, 3398
EcoRV GATATC 2 cut(s) 1919, 3029
EcoT14I CCWWGG 5 cut(s) 98, 1590, 2121, 2621, 3242
EcoT22I ATGCAT 3 cut(s) 1640, 2091, 2667
EcoT38I GRGCYC 1 cut(s) 1742
ErhI CCWWGG 5 cut(s) 98, 1590, 2121, 2621, 3242
Esp3I CGTCTC 1 cut(s) 2861
FaqI GGGAC 7 cut(s) 430, 2373, 2950, 2968, 3518, 3536, 3686
FauI CCCGC 1 cut(s) 406
FbaI TGATCA 1 cut(s) 1285
FblI GTMKAC 2 cut(s) 980, 1109
FokI GGATG 8 cut(s) 32, 235, 602, 1730, 2496, 2981, 3005, 3415
FriOI GRGCYC 1 cut(s) 1742
GsuI CTGGAG 3 cut(s) 321, 1470, 1548
HaeIII GGCC 6 cut(s) 103, 1740, 2009, 2410, 3761, 3874
HapII CCGG 4 cut(s) 70, 104, 1159, 3032
HgaI GACGC 3 cut(s) 29, 116, 1692
Hin1I GRCGYC 1 cut(s) 108
HindIII AAGCTT 2 cut(s) 2709, 3252
HpaII CCGG 4 cut(s) 70, 104, 1159, 3032
Hpy166II GTNNAC 9 cut(s) 981, 1110, 1487, 1502, 2378, 3022, 3038, 3140, 3239
Hpy8I GTNNAC 9 cut(s) 981, 1110, 1487, 1502, 2378, 3022, 3038, 3140, 3239
Hpy99I CGWCG 2 cut(s) 256, 1659
HpyCH4IV ACGT 8 cut(s) 282, 725, 1598, 1692, 1707, 2486, 2746, 3142
HpySE526I ACGT 8 cut(s) 282, 725, 1598, 1692, 1707, 2486, 2746, 3142
Hsp92I GRCGYC 1 cut(s) 108
KpnI GGTACC 1 cut(s) 2678
Ksp22I TGATCA 1 cut(s) 1285
KspI CCGCGG 1 cut(s) 414
LmnI GCTCC 5 cut(s) 72, 580, 865, 1773, 2511
Lsp1109I GCAGC 9 cut(s) 136, 683, 2030, 2762, 3355, 3370, 3392, 3456, 3751
LweI GCATC 9 cut(s) 201, 1340, 1647, 2005, 2710, 3456, 3493, 3646, 3697
MaeII ACGT 8 cut(s) 282, 725, 1598, 1692, 1707, 2486, 2746, 3142
MbiI CCGCTC 2 cut(s) 278, 1003
MfeI CAATTG 1 cut(s) 3914
MflI RGATCY 4 cut(s) 1546, 2557, 3310, 3319
MhlI GDGCHC 3 cut(s) 234, 1030, 1742
MlyI GAGTC 4 cut(s) 127, 155, 3098, 3295
MmeI TCCRAC 4 cut(s) 613, 1021, 2972, 3079
Mph1103I ATGCAT 3 cut(s) 1640, 2091, 2667
MroXI GAANNNNTTC 3 cut(s) 1404, 1896, 3321
MslI CAYNNNNRTG 2 cut(s) 2244, 2268
MspA1I CMGCKG 6 cut(s) 413, 490, 1037, 2942, 3383, 3764
MspI CCGG 4 cut(s) 70, 104, 1159, 3032
MspR9I CCNGG 8 cut(s) 294, 339, 762, 1011, 1449, 2373, 3033, 3400
MunI CAATTG 1 cut(s) 3914
MvaI CCWGG 7 cut(s) 294, 339, 762, 1011, 1449, 2373, 3400
MvnI CGCG 3 cut(s) 40, 131, 413
NciI CCSGG 1 cut(s) 3033
NcoI CCATGG 2 cut(s) 1590, 2121
NheI GCTAGC 1 cut(s) 3394
NlaIV GGNNCC 5 cut(s) 36, 1740, 2676, 3534, 3552
NmeAIII GCCGAG 1 cut(s) 183
NmuCI GTSAC 8 cut(s) 353, 360, 492, 890, 909, 1161, 2213, 3431
NruI TCGCGA 1 cut(s) 131
NsiI ATGCAT 3 cut(s) 1640, 2091, 2667
NspI RCATGY 1 cut(s) 2093
PaeI GCATGC 1 cut(s) 2093
PaeR7I CTCGAG 1 cut(s) 468
PagI TCATGA 1 cut(s) 1396
PcsI WCGNNNNNNNCGW 1 cut(s) 2636
PdmI GAANNNNTTC 3 cut(s) 1404, 1896, 3321
PfeI GAWTC 8 cut(s) 1676, 2222, 2280, 2527, 2837, 2918, 3411, 3835
PinAI ACCGGT 1 cut(s) 1158
Ple19I CGATCG 1 cut(s) 3879
PleI GAGTC 4 cut(s) 127, 154, 3097, 3294
PmaCI CACGTG 1 cut(s) 2747
PmlI CACGTG 1 cut(s) 2747
PpsI GAGTC 4 cut(s) 127, 154, 3097, 3294
Ppu21I YACGTR 1 cut(s) 2747
PpuMI RGGWCCY 2 cut(s) 1428, 3550
PshAI GACNNNNGTC 1 cut(s) 3001
PshBI ATTAAT 2 cut(s) 2807, 3614
Psp1406I AACGTT 2 cut(s) 1598, 2486
Psp5II RGGWCCY 2 cut(s) 1428, 3550
Psp6I CCWGG 7 cut(s) 292, 337, 760, 1009, 1447, 2371, 3398
PspCI CACGTG 1 cut(s) 2747
PspEI GGTNACC 3 cut(s) 360, 890, 1161
PspGI CCWGG 7 cut(s) 292, 337, 760, 1009, 1447, 2371, 3398
PspN4I GGNNCC 5 cut(s) 36, 1740, 2676, 3534, 3552
PspOMI GGGCCC 1 cut(s) 1738
PspPI GGNCC 6 cut(s) 1428, 1738, 1739, 3137, 3532, 3550
PspPPI RGGWCCY 2 cut(s) 1428, 3550
PsrI GAACNNNNNNTAC 2 cut(s) 3572, 3604
PsuI RGATCY 4 cut(s) 1546, 2557, 3310, 3319
PvuI CGATCG 1 cut(s) 3879
PvuII CAGCTG 4 cut(s) 490, 1037, 2942, 3383
RruI TCGCGA 1 cut(s) 131
RseI CAYNNNNRTG 2 cut(s) 2244, 2268
SacII CCGCGG 1 cut(s) 414
Sau96I GGNCC 6 cut(s) 1428, 1738, 1739, 3137, 3532, 3550
ScaI AGTACT 1 cut(s) 2614
SchI GAGTC 4 cut(s) 127, 155, 3098, 3295
ScrFI CCNGG 8 cut(s) 294, 339, 762, 1011, 1449, 2373, 3033, 3400
SduI GDGCHC 3 cut(s) 234, 1030, 1742
SfaNI GCATC 9 cut(s) 201, 1340, 1647, 2005, 2710, 3456, 3493, 3646, 3697
SfcI CTRYAG 2 cut(s) 235, 1772
Sfr274I CTCGAG 1 cut(s) 468
Sfr303I CCGCGG 1 cut(s) 414
SgrBI CCGCGG 1 cut(s) 414
SinI GGWCC 4 cut(s) 1428, 3137, 3532, 3550
SlaI CTCGAG 1 cut(s) 468
SmiMI CAYNNNNRTG 2 cut(s) 2244, 2268
SmlI CTYRAG 3 cut(s) 468, 2381, 3199
SmoI CTYRAG 3 cut(s) 468, 2381, 3199
SpeI ACTAGT 1 cut(s) 3016
SphI GCATGC 1 cut(s) 2093
SspI AATATT 1 cut(s) 1583
StyD4I CCNGG 8 cut(s) 292, 337, 760, 1009, 1447, 2371, 3031, 3398
StyI CCWWGG 5 cut(s) 98, 1590, 2121, 2621, 3242
TaiI ACGT 8 cut(s) 285, 728, 1601, 1695, 1710, 2489, 2749, 3145
TaqII GACCGA 1 cut(s) 612
TatI WGTACW 8 cut(s) 580, 709, 1117, 1387, 2612, 3021, 3744, 4031
TauI GCSGC 7 cut(s) 40, 413, 545, 1214, 1559, 1915, 3764
TfiI GAWTC 8 cut(s) 1676, 2222, 2280, 2527, 2837, 2918, 3411, 3835
TscAI CASTG 4 cut(s) 239, 2453, 2470, 2476
TseFI GTSAC 8 cut(s) 353, 360, 492, 890, 909, 1161, 2213, 3431
TseI GCWGC 9 cut(s) 124, 671, 2018, 2750, 3368, 3380, 3383, 3469, 3764
Tsp45I GTSAC 8 cut(s) 353, 360, 492, 890, 909, 1161, 2213, 3431
TspGWI ACGGA 3 cut(s) 240, 1191, 1487
TspRI CASTG 4 cut(s) 239, 2453, 2470, 2476
VpaK11BI GGWCC 4 cut(s) 1428, 3137, 3532, 3550
VspI ATTAAT 2 cut(s) 2807, 3614
XagI CCTNNNNNAGG 1 cut(s) 1299
XapI RAATTY 5 cut(s) 1493, 1611, 1663, 1896, 2684
XbaI TCTAGA 4 cut(s) 751, 2830, 3313, 3499
XceI RCATGY 1 cut(s) 2093
XcmI CCANNNNNNNNNTGG 2 cut(s) 1037, 3240
XhoI CTCGAG 1 cut(s) 468
XmaJI CCTAGG 1 cut(s) 2621
XmiI GTMKAC 2 cut(s) 980, 1109
XmnI GAANNNNTTC 3 cut(s) 1404, 1896, 3321
ZrmI AGTACT 1 cut(s) 2614
Zsp2I ATGCAT 3 cut(s) 1640, 2091, 2667
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.