RLG00000016244
ERF Family

Probably involved in the RNA silencing pathway and required for the generation of small interfering RNAs (siRNAs)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0000505
Physical Location & Seq
Forward (+)
5061769 .. 5071880
10112 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000016244

Sequence Viewer

Length: 3258 bp
ATGTCCGTCGTGCAGCTACTTGACTCGGTGCAGCAATCGCTGGATCGAATATGCCACCTTAAAAACCAACCACAGCCGAACGACGGCATCAGAAGAAAGCTTGCATTGCTGAGCGAGCAAGACGCCCTCTACGTGCTAAACCAAATTTACAACAGCAGAACTCCCATCAGAACCCTCAATGGATTCATCTCCTTCATGATCAAGGAACTAGAGAATGAATCTTCTTCGTCATCTTCAAGAACAGTTTGGACCCCTCAGAGTCCCACTGCTTCTTCTTCTTCTTCATCATTTACTAGTCCTGCACGGCCTGATCATTCTCAAGGTGAGACAATCCAGTGTGGTTCTCCTGTTAGTCGACAGCCACTCTATTCTTCAGCTTCTCCTTCTCAAGCTTCCCCGACTTCTTCTTATCAGAGCCATTCCTCTCCTACTATTACTGATGCATCATTTTATTCCAGTTCTCAGCCTCAGGATGAGACTCCCAACCCATCCCCGGGGACAGTGTGCAATGGACAACCACCCTATTCCTTCCCTTCTCTCTATCAAATCCTATCTGCTCCTTTTAATACTCTTAGACAACTCTATGAGTGGTTCCAAGGTGAGACTGTCACTCATTCCCCAGTTCTTTACCATCCTGGAGACCATGGACCAGCACACTTAGAAGCTCTGGGAGAACTCGATTTCAGAAAGCAATTTCTGATTTTGAGTTATTCTGGAGGAACGAAGTTAAAAGATGTAATAGAAGCGGAGGCTATTAGGAGCTGGAAAAGTTTGCCAATGGTAAAGTTCGAGACCACAGTTTGGCATGCTTTGGGTGAAAAAAATGTTGGCAAGGCTGACCGACGATCGTATTCTGATTGGGATTCTCAAAGAACACACCAGTACTATTGTTATGTTTCCGTGGATGGGAGTTACAAGTTCAAGGGTCCCTTCCTAGTCAAACCAAGAACTCTCCTACAGAAACAGTTAGGAGATGACAATGTTTTGGAGGTGAAATTTACCCAAGGGGAAGGTTCCAGTGGTACATATAGCAAGTTAATGCGAGAAGGCATTCTTGTTGGTTTGCGGCGATACGCTTTCTTTGTGTTCCGTGCTGGTAACAAAGGAAAGAATATGTGGAAAGACCCAACTTCATCACCTGTGAAATGTTACTTTGTTCGTGGGAGCTCTGATGCAGTGATAGACAAGGGTATAGGCTATAAGTTATCTGGCAAAACGATGCATGAAGGAAGATCTCTTTTCACGCATGCACACACTGGGCCTAGTTTGGCTAGCTACATGGATAGGTTATCTCTCATTTTGTCCAAGACAGTTACTTTAGAAGTAAATTGGTCACATGTCAAGGTGGAATTCATCAAAGACAAAGACTGTCGCGATGAGTCTGGTAATCCCATCTATGTAGATGGAAAACCTCGTATACATACTGATGGCACAGGATTTATATCTGAGGATCTGGCTTTACTTTGTCCTGAGATAGCACGCAAAGGAGAGCAGATCAATAATGAACACATCGAGGGACTTGATGATCCTGATGATGAACTTGAGGACAAAATTCTGGAATGGAAACATCCAGGCACTAGAACTCGGGAACTGCCTTTGCTCATGCAGTTCCGACTTTTTTACAAAGGCCGTGCTATCAAAGGAACTTTTCTTGTCAATAAAGAGCTTCCACCAAAAACTTTGAAGATTCGATCATCAATGGTTAAAGTTGAGCCAGATCCAAAATTGTCAGATCCTCAAACAGTGAATTCACTGGAGATAGTAGGAACAAGTAAAGTTAATAAGAGAACGTATTTGTCGAGGAACTTGATTGCACTTTTATACCATGGAGGGGTGCCAAAAGAATACTTCATCGAGTTACTTATGAAAGCTTTAAAAGAGACTCGTGATGTTTTTAGCAATACAAGCGCGGCATTCAGAGTTGCCCTAAGATGTGGCGAGATCGATGACAATAGTACTGTTGCGAAAATGATCTCCTGTGGTATTCCACTTGAAGAACCATATGTGCAATATCGTCTTTCAACCTTTATGAACATGGAAAAGATCAATCTCAAAAAAGGGAAAATTTGTATTCCTGAATCATACTATTTGATGGGGACAGCTGATCCAACAGGAAAACTTGAAAAGGATGAAGTTTGCATTATCCTAGAAAATGGACAACTACTGTCGGGAGATTTTCTAGTGTACCGGAATCCTGGTTTACACTTTGGTGATATTCATGTCCTAAAGGCCAGATATGTCAAGGAACTAGAATCTGTTGTTGGCAATGCCAAGTACGGTATATTCTTCTCTAGCAAAGGTCCACGTTCCGTTGCTGATGAAATGGCTGGTGGAGACTTTGATGGTGATCTATATTGGATCTCAAGAAACCCTCAGCTATTAAAATCGTTTAAACCAAGTGAACCTTGGATTGGAAAGTCAGCTGCCCAAAAAGTTCATGGTAAAAGACCAACCGAGCTTTCAAAGGATGATCTGGAAGATGAGCTCATCAAACTATTCTTGAAGACTAGGTTTGAACCAAGCTTCGCAGTGTATGAGGCAGCTCAAAACTGGATGGCTTTGATGGATAAATTTTTGACTCTAGGAGATATGAGTATTGATGTGAAAAACAAGCTGAAGGACAAGATACTGCGATTAATTGATATTTACTATGAAGCTCTCGATGCACCAAAAAAAGGTTTTGAGGTTGTAGTTCCAAAAGAATTGAAGGTGCGAGAGTTCCCACATTTCATGGAGAAGAAAAACCAAGAATCCTACCATTCAAGTTCCATTTTGGGATCCATTTATGATAAGGTGGAGGAATTTGAAACAGAAGACAGATCTCTCACAGAAATTCGGAAACTTACTCTTTTTGAAGACGACGTTCCTGAGGACCGCTTGAAAGCATGGGAGAAACGATACGAAGAGTACCGGAAGAAGATGACCTCTGCCATGGGATATGAAGATAAAGAGAGCAAGAATCAGGCAGCCAATATCGTCATAAGAGAGTATAAAGAGATCCTATACGAGGCTGAGGAGTTTGAGGAAAGTAAAAGGGACAGACAACAAATATATAACGACGCACTCGCTATTTATCACGTTTGTTATGACTATGCCAGGAGTGTGAGGACTGAAACTGGGGCACAAGATGTCCGGAAATGTGGCTTTGTCTGGAAAGTTGCAGGCGCAGCTCTATGCCAGCTTCATGAGGACCGGAGTTTGAAACCTGGTGAAAAGGTTTTCAGGATCTTACCCTCTGTTTTGAAGGATATTATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RdRP

Protein Analysis

1086

Amino Acids

123.15

Weight (kDa)

7.11

Isoelectric Point (pI)

41.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
4HB_RdRP3_N PF26249 6 - 69 5.7e-09 RdRP3/4/5, N-terminal four helical bundle domain
RdRP_helical PF26252 219 - 283 8.3e-08 RNA dependent RNA polymerase, helical domain
RdRP PF05183 307 - 932 1.3e-101 RNA dependent RNA polymerase
RdRP_head PF26253 969 - 1064 2.4e-12 RdRP, head domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000505)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G19910 AT2G19910 AT2G19920 AT2G19920 AT2G19920 AT2G19920 AT2G19930 AT2G19930 AT2G19930
fragaria_vesca FvH4_1g05980 FvH4_1g05990 FvH4_1g05990 FvH4_1g09718
malus_domestica MD02G1064400.v1.1 MD15G1195300.v1.1 MD15G1195400.v1.1
prunus_persica Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1
pyrus_communis pycom02g05070 pycom15g17460
rosa_chinensis RchiOBHm_Chr2g0091671 RchiOBHm_Chr2g0091681 RchiOBHm_Chr2g0091691 RchiOBHm_Chr2g0091701 RchiOBHm_Chr2g0091711 RchiOBHm_Chr2g0091721 RchiOBHm_Chr2g0091731 RchiOBHm_Chr5g0014401
rosa_laevigata RLG00000013772 RLG00000016242 RLG00000016244 RLG00000016245 RLG00000016246 RLG00000032105
rosa_multiflora Rmu_co8374041.1_g000001 Rmu_co8478121.1_g000001 Rmu_sc0000897.1_g000005 Rmu_sc0000897.1_g000009
rosa_roxburghii Rroxscaffold_1G00062180 Rroxscaffold_1G00062190 Rroxscaffold_1G00068800 Rroxscaffold_2G00149790 Rroxscaffold_2G00149800 Rroxscaffold_2G00149810
rosa_rugosa Rorug02G0019600 Rorug02G0019700 Rorug02G0019800 Rorug02G0019900 Rorug02G0019900 Rorug02G0020000 Rorug02G0020000 Rorug02G0020100 Rorug02G0020100 Rorug02G0020200 Rorug05G0017800
rosa_samantha Rh2AG064700 Rh2AG064800 Rh2AG064900 Rh2BG063800 Rh2BG063900 Rh2BG064000 Rh2BG064100 Rh2CG065500 Rh2CG065600 Rh2CG065700 Rh2DG063900 Rh2DG064000 Rh2DG064100 Rh2DG064200 Rh2DG381700 Rh5AG056700 Rh5AG056800 Rh5AG056900 Rh5AG112600 Rh5BG109300 Rh5CG063800 Rh5CG121100 Rh5DG053100 Rh5DG108300 Rh6CG100100
rosa_wichuraiana Rw2G005560 Rw2G005580 Rw2G005590 Rw5G005370 Rw5G009770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1834
AccB7I CCANNNNNTGG 1 cut(s) 801
AccI GTMKAC 2 cut(s) 355, 1417
AccII CGCG 2 cut(s) 1374, 1910
AccIII TCCGGA 1 cut(s) 3132
AciI CCGC 4 cut(s) 746, 1066, 1910, 2875
AcsI RAATTY 9 cut(s) 144, 995, 1349, 1551, 1747, 2064, 2570, 2801, 2832
AcuI CTGAAG 2 cut(s) 357, 2636
AcyI GRCGYC 1 cut(s) 123
AfaI GTAC 6 cut(s) 884, 1024, 1957, 2186, 2276, 2909
AfiI CCNNNNNNNGG 9 cut(s) 83, 493, 494, 801, 906, 1831, 2411, 2675, 3007
AflIII ACRYGT 1 cut(s) 1336
AhlI ACTAGT 1 cut(s) 293
AjnI CCWGG 5 cut(s) 634, 1570, 2194, 3095, 3205
AjuI GAANNNNNNNTTGG 8 cut(s) 810, 842, 2244, 2276, 2394, 2426, 2443, 2475
AleI CACNNNNGTG 1 cut(s) 2208
Alw21I GWGCWC 2 cut(s) 1169, 2487
Alw26I GTCTC 7 cut(s) 320, 470, 596, 633, 785, 1874, 2328
Ama87I CYCGRG 2 cut(s) 493, 1584
Aor13HI TCCGGA 1 cut(s) 3132
AoxI GGCC 4 cut(s) 305, 1259, 1627, 2229
ApeKI GCWGC 6 cut(s) 13, 31, 2423, 2540, 2966, 3167
ApoI RAATTY 9 cut(s) 144, 995, 1349, 1551, 1747, 2064, 2570, 2801, 2832
AseI ATTAAT 1 cut(s) 2636
Asp700I GAANNNNTTC 1 cut(s) 1050
AspLEI GCGC 2 cut(s) 1910, 3167
AspS9I GGNCC 7 cut(s) 249, 647, 926, 1259, 2300, 2872, 3190
AsuC2I CCSGG 2 cut(s) 494, 495
AsuHPI GGTGA 8 cut(s) 335, 611, 827, 1003, 1128, 2222, 2357, 3221
AsuNHI GCTAGC 1 cut(s) 1271
AvaI CYCGRG 2 cut(s) 493, 1584
AvaII GGWCC 6 cut(s) 249, 647, 926, 2300, 2872, 3190
AxyI CCTNAGG 2 cut(s) 468, 2868
BaeGI GKGCMC 1 cut(s) 3124
BamHI GGATCC 1 cut(s) 2777
BanI GGYRCC 1 cut(s) 1834
BanII GRGCYC 2 cut(s) 1169, 2487
BauI CACGAG 1 cut(s) 1884
BbsI GAAGAC 3 cut(s) 2510, 2820, 2862
Bbv12I GWGCWC 2 cut(s) 1169, 2487
BbvCI CCTCAGC 2 cut(s) 2373, 3012
BbvI GCAGC 6 cut(s) 25, 43, 2410, 2552, 2978, 3179
BceAI ACGGC 3 cut(s) 100, 320, 1614
BciT130I CCWGG 5 cut(s) 636, 1572, 2196, 3097, 3207
BclI TGATCA 2 cut(s) 198, 310
BcnI CCSGG 2 cut(s) 494, 495
BcoDI GTCTC 7 cut(s) 320, 470, 596, 633, 785, 1874, 2328
BcuI ACTAGT 1 cut(s) 293
BfmI CTRYAG 1 cut(s) 956
BglII AGATCT 2 cut(s) 1232, 2819
BisI GCNGC 8 cut(s) 14, 32, 1067, 1911, 2424, 2541, 2967, 3168
BlpI GCTNAGC 1 cut(s) 110
BlsI GCNGC 8 cut(s) 15, 33, 1068, 1912, 2425, 2542, 2968, 3169
BmcAI AGTACT 2 cut(s) 884, 1957
Bme1390I CCNGG 7 cut(s) 494, 495, 636, 1572, 2196, 3097, 3207
Bme18I GGWCC 6 cut(s) 249, 647, 926, 2300, 2872, 3190
BmeT110I CYCGRG 2 cut(s) 493, 1584
BmgT120I GGNCC 7 cut(s) 249, 647, 926, 1259, 2300, 2872, 3190
BmiI GGNNCC 7 cut(s) 251, 593, 927, 928, 1015, 1836, 2779
BmrFI CCNGG 7 cut(s) 494, 495, 636, 1572, 2196, 3097, 3207
BmrI ACTGGG 3 cut(s) 614, 1266, 3126
BmsI GCATC 6 cut(s) 96, 430, 452, 1162, 1209, 2653
BmtI GCTAGC 1 cut(s) 1275
BmuI ACTGGG 3 cut(s) 614, 1266, 3126
BpiI GAAGAC 3 cut(s) 2510, 2820, 2862
BpmI CTGGAG 3 cut(s) 657, 735, 1775
Bpu10I CCTNAGC 2 cut(s) 2373, 3012
Bpu1102I GCTNAGC 1 cut(s) 110
BpuEI CTTGAG 4 cut(s) 303, 372, 1562, 2347
BpuMI CCSGG 2 cut(s) 494, 495
Bsa29I ATCGAT 1 cut(s) 1944
BsaAI YACGTR 1 cut(s) 133
BsaBI GATNNNNATC 2 cut(s) 1441, 2346
BsaHI GRCGYC 1 cut(s) 123
BsaI GGTCTC 2 cut(s) 633, 785
BsaWI WCCGGW 4 cut(s) 2187, 2910, 3132, 3192
Bsc4I CCNNNNNNNGG 9 cut(s) 83, 493, 494, 801, 906, 1831, 2411, 2675, 3007
Bse1I ACTGG 9 cut(s) 334, 456, 620, 880, 1017, 1261, 1758, 2555, 3121
Bse21I CCTNAGG 2 cut(s) 468, 2868
Bse3DI GCAATG 3 cut(s) 104, 514, 2272
Bse8I GATNNNNATC 2 cut(s) 1441, 2346
BseAI TCCGGA 1 cut(s) 3132
BseBI CCWGG 5 cut(s) 636, 1572, 2196, 3097, 3207
BseCI ATCGAT 1 cut(s) 1944
BseGI GGATG 8 cut(s) 478, 488, 631, 910, 1567, 2134, 2473, 2559
BseJI GATNNNNATC 2 cut(s) 1441, 2346
BseLI CCNNNNNNNGG 9 cut(s) 83, 493, 494, 801, 906, 1831, 2411, 2675, 3007
BseMI GCAATG 3 cut(s) 104, 514, 2272
BseMII CTCAG 9 cut(s) 101, 269, 476, 482, 1437, 1461, 2387, 2859, 3003
BseNI ACTGG 9 cut(s) 334, 456, 620, 880, 1017, 1261, 1758, 2555, 3121
BseRI GAGGAG 1 cut(s) 3029
BseSI GKGCMC 1 cut(s) 3124
BseXI GCAGC 6 cut(s) 25, 43, 2410, 2552, 2978, 3179
BsgI GTGCAG 3 cut(s) 32, 50, 285
Bsh1236I CGCG 2 cut(s) 1374, 1910
Bsh1285I CGRYCG 1 cut(s) 848
BshFI GGCC 4 cut(s) 307, 1261, 1629, 2231
BshNI GGYRCC 1 cut(s) 1834
BshVI ATCGAT 1 cut(s) 1944
BsiEI CGRYCG 1 cut(s) 848
BsiHKAI GWGCWC 2 cut(s) 1169, 2487
BsiHKCI CYCGRG 2 cut(s) 493, 1584
BsiSI CCGG 5 cut(s) 494, 2188, 2911, 3133, 3193
BslFI GGGAC 6 cut(s) 246, 511, 912, 1530, 2110, 3050
BslI CCNNNNNNNGG 9 cut(s) 83, 493, 494, 801, 906, 1831, 2411, 2675, 3007
BsmAI GTCTC 7 cut(s) 320, 470, 596, 633, 785, 1874, 2328
BsmFI GGGAC 6 cut(s) 246, 511, 912, 1530, 2110, 3050
BsmI GAATGC 2 cut(s) 1050, 1913
BsnI GGCC 4 cut(s) 307, 1261, 1629, 2231
Bso31I GGTCTC 2 cut(s) 633, 785
BsoBI CYCGRG 2 cut(s) 493, 1584
Bsp1286I GDGCHC 3 cut(s) 1169, 2487, 3124
Bsp13I TCCGGA 1 cut(s) 3132
Bsp1720I GCTNAGC 1 cut(s) 110
Bsp19I CCATGG 3 cut(s) 643, 1825, 2931
Bsp68I TCGCGA 1 cut(s) 1374
BspACI CCGC 4 cut(s) 746, 1066, 1910, 2875
BspANI GGCC 4 cut(s) 307, 1261, 1629, 2231
BspCNI CTCAG 9 cut(s) 102, 268, 475, 481, 1438, 1462, 2386, 2860, 3004
BspDI ATCGAT 1 cut(s) 1944
BspEI TCCGGA 1 cut(s) 3132
BspFNI CGCG 2 cut(s) 1374, 1910
BspHI TCATGA 2 cut(s) 195, 3184
BspLI GGNNCC 7 cut(s) 251, 593, 927, 928, 1015, 1836, 2779
BspOI GCTAGC 1 cut(s) 1275
BspT107I GGYRCC 1 cut(s) 1834
BspTNI GGTCTC 2 cut(s) 633, 785
BsrDI GCAATG 3 cut(s) 104, 514, 2272
BsrI ACTGG 9 cut(s) 334, 456, 620, 880, 1017, 1261, 1758, 2555, 3121
BssNAI GTATAC 1 cut(s) 1418
BssNI GRCGYC 1 cut(s) 123
BssSI CACGAG 1 cut(s) 1884
BssT1I CCWWGG 6 cut(s) 595, 643, 1003, 1825, 2405, 2931
Bst1107I GTATAC 1 cut(s) 1418
Bst2BI CACGAG 1 cut(s) 1884
Bst2UI CCWGG 5 cut(s) 636, 1572, 2196, 3097, 3207
Bst6I CTCTTC 1 cut(s) 2898
BstACI GRCGYC 1 cut(s) 123
BstBAI YACGTR 1 cut(s) 133
BstC8I GCNNGC 8 cut(s) 102, 116, 807, 1248, 1273, 1480, 3163, 3179
BstDSI CCRYGG 4 cut(s) 643, 900, 1825, 2931
BstENI CCTNNNNNAGG 1 cut(s) 3005
BstF5I GGATG 8 cut(s) 478, 488, 631, 910, 1567, 2134, 2473, 2559
BstFNI CGCG 2 cut(s) 1374, 1910
BstHHI GCGC 2 cut(s) 1910, 3167
BstMAI GTCTC 7 cut(s) 320, 470, 596, 633, 785, 1874, 2328
BstMCI CGRYCG 1 cut(s) 848
BstMWI GCNNNNNNNGC 6 cut(s) 37, 106, 115, 1905, 2663, 3167
BstNI CCWGG 5 cut(s) 636, 1572, 2196, 3097, 3207
BstNSI RCATGY 3 cut(s) 809, 1250, 1340
BstSCI CCNGG 7 cut(s) 492, 493, 634, 1570, 2194, 3095, 3205
BstSFI CTRYAG 1 cut(s) 956
BstSLI GKGCMC 1 cut(s) 3124
BstUI CGCG 2 cut(s) 1374, 1910
BstV1I GCAGC 6 cut(s) 25, 43, 2410, 2552, 2978, 3179
BstV2I GAAGAC 3 cut(s) 2510, 2820, 2862
BstX2I RGATCY 9 cut(s) 1232, 1450, 1717, 1732, 2358, 2777, 2819, 2997, 3225
BstYI RGATCY 9 cut(s) 1232, 1450, 1717, 1732, 2358, 2777, 2819, 2997, 3225
BstZ17I GTATAC 1 cut(s) 1418
Bsu15I ATCGAT 1 cut(s) 1944
Bsu36I CCTNAGG 2 cut(s) 468, 2868
BsuRI GGCC 4 cut(s) 307, 1261, 1629, 2231
BsuTUI ATCGAT 1 cut(s) 1944
BtgI CCRYGG 4 cut(s) 643, 900, 1825, 2931
BtgZI GCGATG 1 cut(s) 1389
BtsCI GGATG 8 cut(s) 478, 488, 631, 910, 1567, 2134, 2473, 2559
BtsI GCAGTG 3 cut(s) 264, 1182, 2535
BtsIMutI CAGTG 9 cut(s) 264, 341, 507, 1024, 1182, 1254, 1749, 1751, 2535
BtuMI TCGCGA 1 cut(s) 1374
Cac8I GCNNGC 8 cut(s) 102, 116, 807, 1248, 1273, 1480, 3163, 3179
CciI TCATGA 2 cut(s) 195, 3184
CfoI GCGC 2 cut(s) 1910, 3167
Cfr13I GGNCC 7 cut(s) 249, 647, 926, 1259, 2300, 2872, 3190
Cfr9I CCCGGG 1 cut(s) 493
ClaI ATCGAT 1 cut(s) 1944
CseI GACGC 2 cut(s) 131, 3068
CsiI ACCWGGT 1 cut(s) 3205
Csp6I GTAC 6 cut(s) 883, 1023, 1956, 2185, 2275, 2908
CviQI GTAC 6 cut(s) 883, 1023, 1956, 2185, 2275, 2908
DraI TTTAAA 2 cut(s) 1875, 2392
Eam1104I CTCTTC 1 cut(s) 2898
EarI CTCTTC 1 cut(s) 2898
Ecl136II GAGCTC 2 cut(s) 1167, 2485
Eco130I CCWWGG 6 cut(s) 595, 643, 1003, 1825, 2405, 2931
Eco24I GRGCYC 2 cut(s) 1169, 2487
Eco31I GGTCTC 2 cut(s) 633, 785
Eco47I GGWCC 6 cut(s) 249, 647, 926, 2300, 2872, 3190
Eco53kI GAGCTC 2 cut(s) 1167, 2485
Eco57I CTGAAG 2 cut(s) 357, 2636
Eco81I CCTNAGG 2 cut(s) 468, 2868
Eco88I CYCGRG 2 cut(s) 493, 1584
EcoICRI GAGCTC 2 cut(s) 1167, 2485
EcoNI CCTNNNNNAGG 1 cut(s) 3005
EcoO109I RGGNCCY 1 cut(s) 926
EcoRI GAATTC 2 cut(s) 1349, 1747
EcoRII CCWGG 5 cut(s) 634, 1570, 2194, 3095, 3205
EcoT14I CCWWGG 6 cut(s) 595, 643, 1003, 1825, 2405, 2931
EcoT22I ATGCAT 2 cut(s) 445, 1224
EcoT38I GRGCYC 2 cut(s) 1169, 2487
ErhI CCWWGG 6 cut(s) 595, 643, 1003, 1825, 2405, 2931
FalI AAGNNNNNCTT 6 cut(s) 914, 946, 1038, 1070, 2389, 2421
FaqI GGGAC 6 cut(s) 246, 511, 912, 1530, 2110, 3050
FauNDI CATATG 1 cut(s) 2002
FbaI TGATCA 2 cut(s) 198, 310
FblI GTMKAC 2 cut(s) 355, 1417
Fnu4HI GCNGC 8 cut(s) 14, 32, 1067, 1911, 2424, 2541, 2967, 3168
FokI GGATG 8 cut(s) 475, 485, 618, 917, 1554, 2141, 2480, 2566
FriOI GRGCYC 2 cut(s) 1169, 2487
Fsp4HI GCNGC 8 cut(s) 14, 32, 1067, 1911, 2424, 2541, 2967, 3168
GlaI GCGC 2 cut(s) 1909, 3166
GluI GCNGC 8 cut(s) 14, 32, 1067, 1911, 2424, 2541, 2967, 3168
GsuI CTGGAG 3 cut(s) 657, 735, 1775
HaeIII GGCC 4 cut(s) 307, 1261, 1629, 2231
HapII CCGG 5 cut(s) 494, 2188, 2911, 3133, 3193
HgaI GACGC 2 cut(s) 131, 3068
HhaI GCGC 2 cut(s) 1910, 3167
Hin1I GRCGYC 1 cut(s) 123
Hin6I GCGC 2 cut(s) 1908, 3165
HinP1I GCGC 2 cut(s) 1908, 3165
HincII GTYRAC 1 cut(s) 356
HindII GTYRAC 1 cut(s) 356
HindIII AAGCTT 4 cut(s) 98, 390, 1869, 2521
HpaII CCGG 5 cut(s) 494, 2188, 2911, 3133, 3193
HphI GGTGA 8 cut(s) 335, 611, 827, 1003, 1128, 2222, 2357, 3221
Hpy166II GTNNAC 6 cut(s) 356, 1418, 2185, 2201, 2303, 2402
Hpy8I GTNNAC 6 cut(s) 356, 1418, 2185, 2201, 2303, 2402
Hpy99I CGWCG 5 cut(s) 11, 86, 846, 2864, 3062
HpyCH4IV ACGT 5 cut(s) 132, 1790, 2305, 2862, 3078
HpyF10VI GCNNNNNNNGC 6 cut(s) 37, 106, 115, 1905, 2663, 3167
HpySE526I ACGT 5 cut(s) 132, 1790, 2305, 2862, 3078
Hsp92I GRCGYC 1 cut(s) 123
HspAI GCGC 2 cut(s) 1908, 3165
KflI GGGWCCC 1 cut(s) 926
Kpn2I TCCGGA 1 cut(s) 3132
Ksp22I TGATCA 2 cut(s) 198, 310
LmnI GCTCC 3 cut(s) 562, 759, 1164
Lsp1109I GCAGC 6 cut(s) 25, 43, 2410, 2552, 2978, 3179
LweI GCATC 6 cut(s) 96, 430, 452, 1162, 1209, 2653
MabI ACCWGGT 1 cut(s) 3205
MaeII ACGT 5 cut(s) 132, 1790, 2305, 2862, 3078
MaeIII GTNAC 7 cut(s) 607, 911, 1097, 1148, 1312, 1332, 1857
MflI RGATCY 9 cut(s) 1232, 1450, 1717, 1732, 2358, 2777, 2819, 2997, 3225
MhlI GDGCHC 3 cut(s) 1169, 2487, 3124
MlyI GAGTC 6 cut(s) 17, 268, 472, 1388, 1876, 2572
MmeI TCCRAC 2 cut(s) 1636, 2132
Mph1103I ATGCAT 2 cut(s) 445, 1224
MroI TCCGGA 1 cut(s) 3132
MroXI GAANNNNTTC 1 cut(s) 1050
MslI CAYNNNNRTG 2 cut(s) 1425, 2208
MspA1I CMGCKG 2 cut(s) 2102, 2423
MspI CCGG 5 cut(s) 494, 2188, 2911, 3133, 3193
MspR9I CCNGG 7 cut(s) 494, 495, 636, 1572, 2196, 3097, 3207
MssI GTTTAAAC 1 cut(s) 2392
Mva1269I GAATGC 2 cut(s) 1050, 1913
MvaI CCWGG 5 cut(s) 636, 1572, 2196, 3097, 3207
MvnI CGCG 2 cut(s) 1374, 1910
MwoI GCNNNNNNNGC 6 cut(s) 37, 106, 115, 1905, 2663, 3167
NciI CCSGG 2 cut(s) 494, 495
NcoI CCATGG 3 cut(s) 643, 1825, 2931
NdeI CATATG 1 cut(s) 2002
NheI GCTAGC 1 cut(s) 1271
NlaIV GGNNCC 7 cut(s) 251, 593, 927, 928, 1015, 1836, 2779
NmuCI GTSAC 2 cut(s) 607, 1332
NruI TCGCGA 1 cut(s) 1374
NsiI ATGCAT 2 cut(s) 445, 1224
NspI RCATGY 3 cut(s) 809, 1250, 1340
OliI CACNNNNGTG 1 cut(s) 2208
PaeI GCATGC 2 cut(s) 809, 1250
PagI TCATGA 2 cut(s) 195, 3184
PciI ACATGT 1 cut(s) 1336
PcsI WCGNNNNNNNCGW 2 cut(s) 129, 1796
PctI GAATGC 2 cut(s) 1050, 1913
PdmI GAANNNNTTC 1 cut(s) 1050
PfeI GAWTC 9 cut(s) 183, 218, 863, 1687, 2078, 2191, 2252, 2750, 2959
PflMI CCANNNNNTGG 1 cut(s) 801
PfoI TCCNGGA 1 cut(s) 634
PkrI GCNGC 8 cut(s) 15, 33, 1068, 1912, 2425, 2542, 2968, 3169
Ple19I CGATCG 1 cut(s) 848
PleI GAGTC 6 cut(s) 17, 267, 472, 1387, 1876, 2572
PmeI GTTTAAAC 1 cut(s) 2392
PpsI GAGTC 6 cut(s) 17, 267, 472, 1387, 1876, 2572
Ppu21I YACGTR 1 cut(s) 133
PpuMI RGGWCCY 1 cut(s) 926
PscI ACATGT 1 cut(s) 1336
PshBI ATTAAT 1 cut(s) 2636
Psp124BI GAGCTC 2 cut(s) 1169, 2487
Psp5II RGGWCCY 1 cut(s) 926
Psp6I CCWGG 5 cut(s) 634, 1570, 2194, 3095, 3205
PspGI CCWGG 5 cut(s) 634, 1570, 2194, 3095, 3205
PspN4I GGNNCC 7 cut(s) 251, 593, 927, 928, 1015, 1836, 2779
PspPI GGNCC 7 cut(s) 249, 647, 926, 1259, 2300, 2872, 3190
PspPPI RGGWCCY 1 cut(s) 926
PsuI RGATCY 9 cut(s) 1232, 1450, 1717, 1732, 2358, 2777, 2819, 2997, 3225
PvuI CGATCG 1 cut(s) 848
PvuII CAGCTG 2 cut(s) 2102, 2423
RruI TCGCGA 1 cut(s) 1374
RsaI GTAC 6 cut(s) 884, 1024, 1957, 2186, 2276, 2909
RsaNI GTAC 6 cut(s) 883, 1023, 1956, 2185, 2275, 2908
RseI CAYNNNNRTG 2 cut(s) 1425, 2208
SacI GAGCTC 2 cut(s) 1169, 2487
SalI GTCGAC 1 cut(s) 354
SatI GCNGC 8 cut(s) 14, 32, 1067, 1911, 2424, 2541, 2967, 3168
Sau96I GGNCC 7 cut(s) 249, 647, 926, 1259, 2300, 2872, 3190
ScaI AGTACT 2 cut(s) 884, 1957
SchI GAGTC 6 cut(s) 17, 268, 472, 1388, 1876, 2572
ScrFI CCNGG 7 cut(s) 494, 495, 636, 1572, 2196, 3097, 3207
SduI GDGCHC 3 cut(s) 1169, 2487, 3124
SexAI ACCWGGT 1 cut(s) 3205
SfaNI GCATC 6 cut(s) 96, 430, 452, 1162, 1209, 2653
SfcI CTRYAG 1 cut(s) 956
SinI GGWCC 6 cut(s) 249, 647, 926, 2300, 2872, 3190
SmaI CCCGGG 1 cut(s) 495
SmiMI CAYNNNNRTG 2 cut(s) 1425, 2208
SmlI CTYRAG 4 cut(s) 318, 387, 1541, 2362
SmoI CTYRAG 4 cut(s) 318, 387, 1541, 2362
SpeI ACTAGT 1 cut(s) 293
SphI GCATGC 2 cut(s) 809, 1250
SsiI CCGC 4 cut(s) 746, 1066, 1910, 2875
SstI GAGCTC 2 cut(s) 1169, 2487
StyD4I CCNGG 7 cut(s) 492, 493, 634, 1570, 2194, 3095, 3205
StyI CCWWGG 6 cut(s) 595, 643, 1003, 1825, 2405, 2931
TaiI ACGT 5 cut(s) 135, 1793, 2308, 2865, 3081
TaqII GACCGA 1 cut(s) 855
TatI WGTACW 2 cut(s) 882, 1955
TauI GCSGC 2 cut(s) 1069, 1913
TfiI GAWTC 9 cut(s) 183, 218, 863, 1687, 2078, 2191, 2252, 2750, 2959
TscAI CASTG 9 cut(s) 271, 341, 507, 1024, 1182, 1261, 1749, 1758, 2535
TseFI GTSAC 2 cut(s) 607, 1332
TseI GCWGC 6 cut(s) 13, 31, 2423, 2540, 2966, 3167
Tsp45I GTSAC 2 cut(s) 607, 1332
TspGWI ACGGA 3 cut(s) 889, 1079, 2299
TspMI CCCGGG 1 cut(s) 493
TspRI CASTG 9 cut(s) 271, 341, 507, 1024, 1182, 1261, 1749, 1758, 2535
Van91I CCANNNNNTGG 1 cut(s) 801
VpaK11BI GGWCC 6 cut(s) 249, 647, 926, 2300, 2872, 3190
VspI ATTAAT 1 cut(s) 2636
XagI CCTNNNNNAGG 1 cut(s) 3005
XapI RAATTY 9 cut(s) 144, 995, 1349, 1551, 1747, 2064, 2570, 2801, 2832
XceI RCATGY 3 cut(s) 809, 1250, 1340
XcmI CCANNNNNNNNNTGG 2 cut(s) 2403, 2435
XmaI CCCGGG 1 cut(s) 493
XmiI GTMKAC 2 cut(s) 355, 1417
XmnI GAANNNNTTC 1 cut(s) 1050
ZrmI AGTACT 2 cut(s) 884, 1957
Zsp2I ATGCAT 2 cut(s) 445, 1224
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.