FvH4_1g05980
ERF Family

Probably involved in the RNA silencing pathway and required for the generation of small interfering RNAs (siRNAs)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0000505
Physical Location & Seq
Forward (+)
3184295 .. 3194521
10227 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g05980.t1

Sequence Viewer

Length: 3678 bp
ATGAAGCGTGTTTTGGATGAGCAGCAAGAGCCGCGTCTACCTCCGTCTGTGGAGTTAAAGCTCCGGCAAATCTGTGAGAGCAAAATGCAACCTTGGCCGGATGATGACGTCATACGGCAGCTGGCGGCTCTGAGTGAGGAGTCGGCAAATGAGACCCTGGAGAGAATCTCAACCAGTAAAAACATCAGAACCCTCGGCGGTTATATCAAGTGGATGATCCGGCAGCAATGTACGGCTGCTTCTTCTCCGTCGCCGTCCAAAACGGTGCGTTCTGCTCATGTTCAAAGCCCTGGTGCTAGTTCAATTGTTCCTCGGAGACTTCAGTTTGGTACTCCAGGTGAGGCGTTTGGTGTTGTAGAGCAACCCGGAAGCTGTAATGGAAGCTGTGTTGCTCATGGACAAGTTGATGAGAGGTTTCGAGGTGAGGTGTATGGTGTTGTGGAGCAACCCGGAAGCTGTAATGGAAGCTGTGTTGCTCATGTACAAGTTGATGAGAGGTCTCGAGGTGAGGCAGTTAGTGACAGGTCACCTTACTGCTATGGTTCTCCATTGAAAGCAACAGGGAGTTGTTCTTATCAAAGATTGTCTACTCATGCACAAGTTCATGAGAGGTCACAAGGTGAGGCATTTAATCACCTGCCACCTGTTAGAGGTCAACCACCTTATTCATCGGGTTCTCCGATGAAACTTGCAGGCACTTCTTCTCAAAGACTGTCTGGTCCTGGCCAACTTGATGATGGGTTTCAAGGTGAGGCATTTACTGTTCTAGAGCAACCCGGAAGCAACAACAATACACAATCTCCTTATTCTCCAGTGAAAGTGGCACACACTTGTTCTTGTCAAAGCCCATCTGCTTCTGTTGCTCGTGTACAACTTGAGAAGAGGTTTCAGGGTGGGGTATTTACTGATCAGACACCTGTTCAAGGACTATCAGCATATGCTGGTTCTCCATCAAAAGCCGTCTGCATTTCTCCATATCAGAGACCGTCATCTGATGTTATTCCTGTACAACTTCATCCGAGGTTTCAATGTGAGGGTATCACTAGGTCCTCAGTTATAGACAAACCTGAAGACCATGGAGAAGCACACTTGGCAGCTCTGGGAGAACTTGAATATAGAAGACAATTTCTCATATTGAGTTACGCTGGAGGAATGAAGTTGAGGGATCTTATAGTGGCAGAGGCCATTAGGCATTGGAAAGATTTGCCAATGGTAATGTTTGAGAGCACAGTTTGGGAACATCTAGGTCGAAAATGCATCAGCACTGAAGACCGTCGATTGTATTTTGATTGGGATTCTGGAAGCACACATGTCTATCATTGTTATGTCAACGTGGATGGGAGTTACAGATTCAAGGGCCCATATCTATATAAGATAAGAAGGACGCTCCTACAGAAAGAATTAGGAGATGATAATGTTCTAATGGTGAAGTTTGCTAAAGAAGAGGTGCAGAGAGGTCAAAGTGGTGCTTGCCACAATTATCCTACGTATATGAAGGTTGCAAGAGAAGGGATTCTTGTTGGTTTGAGGCGATATCGTTTCTTTGTGTTCAAAGATGGTGGTAATGAGGAAAAAAAGAAAAACCCAACCTCATCACCTGTGAAATGTTACTTTGTTCGAGTGGCCTCTGATGCAGCCATAGACAAAGGTGTCTATTATAGATTATCTCATATGACGATGTATGAAGCCAGATGTCTTTTCATGCATGCACATACTCTGGCTAGTATCGATAACTACATGGCTAGGTTTTCACTCATTTTGTCCAAGACAATGAGCTTAAAAGCAGATTGGTCACTTGTCAAGGTTGAGATAATCGATGAAGAATACTGTCTTGATGAATCTGGTAATTGTATCTATAGAGATGAAAAACCTCTTATACATACAGATGGAACTGGATTCATATCTGAGGATTTGGCTTTACTTTGTCCAAAGATAGAAGCCAAAGGACAGTGGATAAGCGATGAACACATGAAGGGACTTCCTGATCCTGATGAACTTGATGATACGGGAAAGAACCGGGCAGGTCTTAGAACTGAAGAGCCGCCTTTGCTCATACAGTGCCGACTTTTTCACAATGGCAGTGCTTCTAAAGGAACGCTCCTTGTCAATAAGAAGCTTCAACCAAACAGAATCCAGATTCGACCATCAATGCTTAAAGTTAAGACAGATCCAGTGATTTCCAATAATCAAACACTGAACTCTTTGGAAATAGTTGGCACAAGTACTCCTCCTAGGAATACTCATTTGTCAAGGATACTAATTGCACTTCTAGTGCATGGAGGGGTGCCAGAGGAATTTTTCATGCAGATACTCAACAAGGCTTTGGAAGATTCTCATGGTGTTTTCTGCAAGAAACGTGCAGCACTTAAAGTTGCTGAGAAGTATGGTGAGATGGATGACAATTTCTTGGGAAGATTAATGATCTCATTTGGGATACCTCTAGAAGAGTCATACTTGCGGTATCGTCTGTCAGTCCTCATGAAAGAGGAAAACAACAGTCTCAGGAAAGGGAGACTTTATATTCCTGATTCGTATAATTTGATGGGGACAGCTGATCCAACAGGGACTTTAAAAGAAGATGAAGTCTGCGTTATCCTGAAGGATGGACAACTGTCTGGGAAGGTACTAGTTTACAGGTATCCAGGTTTACACATTGGGGATATTCATGTTCTAAAGGCCACCTATGTAAAGGAGATAGAATATATTGTTGGAAATGCCAAATACGGTATATTCTTCTCTTGCAAAGGTCCACGTTCCATTGCTGATGAAATGGGTGGTGGAGATTTTGATGGTGATCTCTACTGGGTCTCAAGAAACCCTCAGCTATTGGAGTGGTACAAACCAAGTGAACCTTGGATTGAAGCTTCAATATCAACTAAAAAAAATCCCAGTAGGTGTCCATCTGACCATTCGCCTGAAGATCTAGAGGATGTTCTGTTCAGGTCATTCCTGACAACTAGGTTTGAACCAAGTTTTGCTATGAGTGAGGCTGCTGATAACTGGCTGGCTTGGATGGATCACTTCTTGACTCTAGGAGATAGTAACATTCATGAAAAGAATAATGTGAAGGAAAAGATACTGCAGCTGGTTGACTTGTACTACACAGCACTAGATGCTGCAAAGAAAGGCGTAAAGGTTGACGTCCCAAAAGAATTGAAGGTCTCATGTTTCCCCCATTACATGGGGAAGAACAGGTCTTACCCTTCAACTTCCATTTTGGGATCAATTTATAACAGGGTGGATGAGCATCAAACAGAGGACCGTTCTCTCAAAGAAGTCGAGAAACTTCCTGCTTTTGATGAAAATGAAGTCCCTGATGCCTGCTTGGACAAATGGGCGGAACTACATAGACAGTATAGGAAGGAGATGGCCTCAGCTTTGGACGACGATGATAAACAAAGCAAAAATCAGTCTGCCGATGTGGTCATAAGGAAATATAGAAAGATTCTATACGAGGCAAATGACTTTGAGGAGAGTAGAAGGCCGATTGAGGAGATATATAACGAGGCACATGCTATATATCATGTGTGTTATGACCATGCGAAGAGGGTAGGCAATGTGAAGGTGTGTTCCTTTGCGTGGAGAGTCGCAGGTAAAGCTCTTTGCCAGCTTCACGCTAGGAACAACAACGAAAGGTTTTGTACAATAGTATCCTCTGTTTTGATGGAGATTTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RdRP

Protein Analysis

1226

Amino Acids

138.02

Weight (kDa)

6.77

Isoelectric Point (pI)

46.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
4HB_RdRP3_N PF26249 10 - 83 2.3e-08 RdRP3/4/5, N-terminal four helical bundle domain
RdRP_helical PF26252 363 - 427 3.8e-07 RNA dependent RNA polymerase, helical domain
RdRP PF05183 450 - 1080 1.8e-102 RNA dependent RNA polymerase
RdRP_head PF26253 1101 - 1211 1.4e-13 RdRP, head domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000505)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G19910 AT2G19910 AT2G19920 AT2G19920 AT2G19920 AT2G19920 AT2G19930 AT2G19930 AT2G19930
fragaria_vesca FvH4_1g05980 FvH4_1g05990 FvH4_1g05990 FvH4_1g09718
malus_domestica MD02G1064400.v1.1 MD15G1195300.v1.1 MD15G1195400.v1.1
prunus_persica Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1
pyrus_communis pycom02g05070 pycom15g17460
rosa_chinensis RchiOBHm_Chr2g0091671 RchiOBHm_Chr2g0091681 RchiOBHm_Chr2g0091691 RchiOBHm_Chr2g0091701 RchiOBHm_Chr2g0091711 RchiOBHm_Chr2g0091721 RchiOBHm_Chr2g0091731 RchiOBHm_Chr5g0014401
rosa_laevigata RLG00000013772 RLG00000016242 RLG00000016244 RLG00000016245 RLG00000016246 RLG00000032105
rosa_multiflora Rmu_co8374041.1_g000001 Rmu_co8478121.1_g000001 Rmu_sc0000897.1_g000005 Rmu_sc0000897.1_g000009
rosa_roxburghii Rroxscaffold_1G00062180 Rroxscaffold_1G00062190 Rroxscaffold_1G00068800 Rroxscaffold_2G00149790 Rroxscaffold_2G00149800 Rroxscaffold_2G00149810
rosa_rugosa Rorug02G0019600 Rorug02G0019700 Rorug02G0019800 Rorug02G0019900 Rorug02G0019900 Rorug02G0020000 Rorug02G0020000 Rorug02G0020100 Rorug02G0020100 Rorug02G0020200 Rorug05G0017800
rosa_samantha Rh2AG064700 Rh2AG064800 Rh2AG064900 Rh2BG063800 Rh2BG063900 Rh2BG064000 Rh2BG064100 Rh2CG065500 Rh2CG065600 Rh2CG065700 Rh2DG063900 Rh2DG064000 Rh2DG064100 Rh2DG064200 Rh2DG381700 Rh5AG056700 Rh5AG056800 Rh5AG056900 Rh5AG112600 Rh5BG109300 Rh5CG063800 Rh5CG121100 Rh5DG053100 Rh5DG108300 Rh6CG100100
rosa_wichuraiana Rw2G005560 Rw2G005580 Rw2G005590 Rw5G005370 Rw5G009770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3231
AarI CACCTGC 1 cut(s) 645
AasI GACNNNNNNGTC 2 cut(s) 717, 1649
AatII GACGTC 2 cut(s) 111, 3144
Acc36I ACCTGC 3 cut(s) 645, 2012, 3581
AccB1I GGYRCC 1 cut(s) 2284
AccB7I CCANNNNNTGG 1 cut(s) 3181
AccI GTMKAC 2 cut(s) 37, 587
AccII CGCG 1 cut(s) 34
AciI CCGC 6 cut(s) 32, 125, 198, 2042, 2458, 3338
AclWI GGATC 7 cut(s) 211, 1173, 1979, 2162, 2549, 3024, 3229
AcoI YGGCCR 2 cut(s) 95, 724
AcsI RAATTY 1 cut(s) 2294
AcuI CTGAAG 6 cut(s) 305, 1089, 1287, 2055, 2618, 2937
AcyI GRCGYC 2 cut(s) 108, 3141
AdeI CACNNNGTG 1 cut(s) 620
AfiI CCNNNNNNNGG 4 cut(s) 650, 923, 3181, 3579
AflIII ACRYGT 1 cut(s) 1309
AhlI ACTAGT 1 cut(s) 2626
AjnI CCWGG 5 cut(s) 156, 289, 334, 721, 2641
AjuI GAANNNNNNNTTGG 3 cut(s) 28, 2691, 2723
AloI GAACNNNNNNTCC 2 cut(s) 2921, 2953
Alw21I GWGCWC 1 cut(s) 1229
Alw26I GTCTC 8 cut(s) 146, 310, 504, 976, 2504, 2506, 2812, 3166
AlwI GGATC 7 cut(s) 211, 1173, 1979, 2162, 2549, 3024, 3229
Ama87I CYCGRG 1 cut(s) 501
AoxI GGCC 8 cut(s) 95, 724, 1182, 1357, 1623, 2676, 3369, 3482
ApaI GGGCCC 1 cut(s) 1361
ApoI RAATTY 1 cut(s) 2294
AseI ATTAAT 1 cut(s) 2417
Asp700I GAANNNNTTC 1 cut(s) 1512
AspA2I CCTAGG 1 cut(s) 2231
AspS9I GGNCC 6 cut(s) 719, 1047, 1357, 1358, 2747, 3259
AsuC2I CCSGG 4 cut(s) 366, 450, 777, 2018
AvaI CYCGRG 1 cut(s) 501
AvaII GGWCC 4 cut(s) 719, 1047, 2747, 3259
AvrII CCTAGG 1 cut(s) 2231
BaeGI GKGCMC 1 cut(s) 1361
BaeI ACNNNNGTAYC 2 cut(s) 3633, 3666
BalI TGGCCA 1 cut(s) 726
BanI GGYRCC 1 cut(s) 2284
BanII GRGCYC 1 cut(s) 1361
BarI GAAGNNNNNNTAC 2 cut(s) 223, 255
BauI CACGAG 1 cut(s) 864
BbsI GAAGAC 3 cut(s) 1077, 1126, 1275
Bbv12I GWGCWC 1 cut(s) 1229
BbvCI CCTCAGC 2 cut(s) 2820, 3373
BceAI ACGGC 4 cut(s) 131, 238, 249, 944
BcgI CGANNNNNNTGC 2 cut(s) 3494, 3528
BciT130I CCWGG 5 cut(s) 158, 291, 336, 723, 2643
BciVI GTATCC 4 cut(s) 2247, 2427, 2649, 3661
BclI TGATCA 1 cut(s) 907
BcnI CCSGG 4 cut(s) 366, 450, 777, 2018
BcoDI GTCTC 8 cut(s) 146, 310, 504, 976, 2504, 2506, 2812, 3166
BcuI ACTAGT 1 cut(s) 2626
BfmI CTRYAG 3 cut(s) 1391, 1855, 3080
BfuAI ACCTGC 3 cut(s) 645, 2012, 3581
BfuI GTATCC 4 cut(s) 2247, 2427, 2649, 3661
BglII AGATCT 1 cut(s) 2920
BlnI CCTAGG 1 cut(s) 2231
BmcAI AGTACT 1 cut(s) 2224
Bme1390I CCNGG 9 cut(s) 158, 291, 336, 366, 450, 723, 777, 2018, 2643
Bme18I GGWCC 4 cut(s) 719, 1047, 2747, 3259
BmeT110I CYCGRG 1 cut(s) 501
BmgT120I GGNCC 6 cut(s) 719, 1047, 1357, 1358, 2747, 3259
BmiI GGNNCC 2 cut(s) 1359, 2286
BmrFI CCNGG 9 cut(s) 158, 291, 336, 366, 450, 723, 777, 2018, 2643
BmrI ACTGGG 2 cut(s) 2812, 2883
BmsI GCATC 5 cut(s) 1266, 1621, 3103, 3256, 3307
BmuI ACTGGG 2 cut(s) 2812, 2883
BoxI GACNNNNGTC 1 cut(s) 2611
BpiI GAAGAC 3 cut(s) 1077, 1126, 1275
BplI GAGNNNNNCTC 4 cut(s) 152, 184, 3356, 3388
BpmI CTGGAG 4 cut(s) 179, 318, 795, 1167
Bpu10I CCTNAGC 2 cut(s) 2820, 3373
BpuEI CTTGAG 2 cut(s) 896, 2794
BpuMI CCSGG 4 cut(s) 366, 450, 777, 2018
Bsa29I ATCGAT 2 cut(s) 1728, 1815
BsaAI YACGTR 1 cut(s) 1488
BsaBI GATNNNNATC 3 cut(s) 1900, 2793, 3246
BsaHI GRCGYC 2 cut(s) 108, 3141
BsaI GGTCTC 5 cut(s) 146, 504, 976, 2812, 3166
BsaJI CCNNGG 9 cut(s) 92, 156, 193, 289, 311, 1019, 1075, 2231, 2852
BsaXI ACNNNNNCTCC 5 cut(s) 784, 814, 2209, 2239, 3659
Bsc4I CCNNNNNNNGG 4 cut(s) 650, 923, 3181, 3579
Bse1I ACTGG 7 cut(s) 174, 812, 1897, 2171, 2807, 2889, 3005
Bse3DI GCAATG 3 cut(s) 233, 2757, 3562
Bse8I GATNNNNATC 3 cut(s) 1900, 2793, 3246
BseBI CCWGG 5 cut(s) 158, 291, 336, 723, 2643
BseCI ATCGAT 2 cut(s) 1728, 1815
BseDI CCNNGG 9 cut(s) 92, 156, 193, 289, 311, 1019, 1075, 2231, 2852
BseJI GATNNNNATC 3 cut(s) 1900, 2793, 3246
BseLI CCNNNNNNNGG 4 cut(s) 650, 923, 3181, 3579
BseMI GCAATG 3 cut(s) 233, 2757, 3562
BseMII CTCAG 7 cut(s) 122, 1065, 1896, 2367, 2515, 2834, 3387
BseNI ACTGG 7 cut(s) 174, 812, 1897, 2171, 2807, 2889, 3005
BseRI GAGGAG 4 cut(s) 152, 2217, 3485, 3506
BseSI GKGCMC 1 cut(s) 1361
BsgI GTGCAG 2 cut(s) 1469, 2379
Bsh1236I CGCG 1 cut(s) 34
BshFI GGCC 8 cut(s) 97, 726, 1184, 1359, 1625, 2678, 3371, 3484
BshNI GGYRCC 1 cut(s) 2284
BshVI ATCGAT 2 cut(s) 1728, 1815
BsiHKAI GWGCWC 1 cut(s) 1229
BsiHKCI CYCGRG 1 cut(s) 501
BsiSI CCGG 7 cut(s) 64, 98, 220, 366, 450, 777, 2017
BslFI GGGAC 5 cut(s) 1989, 2560, 2578, 3128, 3296
BslI CCNNNNNNNGG 4 cut(s) 650, 923, 3181, 3579
BsmAI GTCTC 8 cut(s) 146, 310, 504, 976, 2504, 2506, 2812, 3166
BsmFI GGGAC 5 cut(s) 1989, 2560, 2578, 3128, 3296
BsnI GGCC 8 cut(s) 97, 726, 1184, 1359, 1625, 2678, 3371, 3484
Bso31I GGTCTC 5 cut(s) 146, 504, 976, 2812, 3166
BsoBI CYCGRG 1 cut(s) 501
Bsp120I GGGCCC 1 cut(s) 1357
Bsp1286I GDGCHC 2 cut(s) 1229, 1361
Bsp1407I TGTACA 4 cut(s) 481, 868, 1006, 3641
Bsp19I CCATGG 1 cut(s) 1075
BspACI CCGC 6 cut(s) 32, 125, 198, 2042, 2458, 3338
BspANI GGCC 8 cut(s) 97, 726, 1184, 1359, 1625, 2678, 3371, 3484
BspCNI CTCAG 7 cut(s) 123, 1064, 1897, 2368, 2514, 2833, 3386
BspDI ATCGAT 2 cut(s) 1728, 1815
BspFNI CGCG 1 cut(s) 34
BspHI TCATGA 3 cut(s) 604, 2478, 3049
BspLI GGNNCC 2 cut(s) 1359, 2286
BspMAI CTGCAG 1 cut(s) 3084
BspMI ACCTGC 3 cut(s) 645, 2012, 3581
BspPI GGATC 7 cut(s) 211, 1173, 1979, 2162, 2549, 3024, 3229
BspQI GCTCTTC 1 cut(s) 2031
BspT107I GGYRCC 1 cut(s) 2284
BspTNI GGTCTC 5 cut(s) 146, 504, 976, 2812, 3166
BsrDI GCAATG 3 cut(s) 233, 2757, 3562
BsrGI TGTACA 4 cut(s) 481, 868, 1006, 3641
BsrI ACTGG 7 cut(s) 174, 812, 1897, 2171, 2807, 2889, 3005
BssECI CCNNGG 9 cut(s) 92, 156, 193, 289, 311, 1019, 1075, 2231, 2852
BssNI GRCGYC 2 cut(s) 108, 3141
BssSI CACGAG 1 cut(s) 864
BssT1I CCWWGG 4 cut(s) 92, 1075, 2231, 2852
Bst2BI CACGAG 1 cut(s) 864
Bst2UI CCWGG 5 cut(s) 158, 291, 336, 723, 2643
Bst6I CTCTTC 5 cut(s) 875, 1437, 2031, 2439, 3539
BstACI GRCGYC 2 cut(s) 108, 3141
BstAPI GCANNNNNTGC 1 cut(s) 3113
BstAUI TGTACA 4 cut(s) 481, 868, 1006, 3641
BstBAI YACGTR 1 cut(s) 1488
BstC8I GCNNGC 7 cut(s) 123, 694, 1471, 1707, 3006, 3322, 3608
BstDEI CTNAG 8 cut(s) 131, 1051, 1905, 2027, 2376, 2501, 2820, 3373
BstDSI CCRYGG 1 cut(s) 1075
BstEII GGTNACC 1 cut(s) 525
BstENI CCTNNNNNAGG 2 cut(s) 648, 921
BstFNI CGCG 1 cut(s) 34
BstMAI GTCTC 8 cut(s) 146, 310, 504, 976, 2504, 2506, 2812, 3166
BstMWI GCNNNNNNNGC 9 cut(s) 28, 31, 94, 860, 1091, 1631, 2047, 3113, 3596
BstNI CCWGG 5 cut(s) 158, 291, 336, 723, 2643
BstNSI RCATGY 3 cut(s) 1313, 1709, 3515
BstPAI GACNNNNGTC 1 cut(s) 2611
BstPI GGTNACC 1 cut(s) 525
BstSCI CCNGG 9 cut(s) 156, 289, 334, 364, 448, 721, 775, 2016, 2641
BstSFI CTRYAG 3 cut(s) 1391, 1855, 3080
BstSLI GKGCMC 1 cut(s) 1361
BstSNI TACGTA 1 cut(s) 1488
BstUI CGCG 1 cut(s) 34
BstV2I GAAGAC 3 cut(s) 1077, 1126, 1275
BstX2I RGATCY 3 cut(s) 1165, 2167, 2920
BstYI RGATCY 3 cut(s) 1165, 2167, 2920
Bsu15I ATCGAT 2 cut(s) 1728, 1815
BsuI GTATCC 4 cut(s) 2247, 2427, 2649, 3661
BsuRI GGCC 8 cut(s) 97, 726, 1184, 1359, 1625, 2678, 3371, 3484
BsuTUI ATCGAT 2 cut(s) 1728, 1815
BtgI CCRYGG 1 cut(s) 1075
BtgZI GCGATG 1 cut(s) 1974
BtsI GCAGTG 1 cut(s) 2086
BtsIMutI CAGTG 7 cut(s) 819, 1263, 1955, 2063, 2086, 2178, 2192
BveI ACCTGC 3 cut(s) 645, 2012, 3581
Cac8I GCNNGC 7 cut(s) 123, 694, 1471, 1707, 3006, 3322, 3608
CciI TCATGA 3 cut(s) 604, 2478, 3049
Cfr13I GGNCC 6 cut(s) 719, 1047, 1357, 1358, 2747, 3259
ClaI ATCGAT 2 cut(s) 1728, 1815
CseI GACGC 2 cut(s) 23, 1393
CspCI CAANNNNNGTGG 2 cut(s) 1540, 1575
DdeI CTNAG 8 cut(s) 131, 1051, 1905, 2027, 2376, 2501, 2820, 3373
DraI TTTAAA 1 cut(s) 2571
DraIII CACNNNGTG 1 cut(s) 620
DrdI GACNNNNNNGTC 2 cut(s) 717, 1649
DseDI GACNNNNNNGTC 2 cut(s) 717, 1649
EaeI YGGCCR 2 cut(s) 95, 724
Eam1104I CTCTTC 5 cut(s) 875, 1437, 2031, 2439, 3539
EarI CTCTTC 5 cut(s) 875, 1437, 2031, 2439, 3539
EciI GGCGGA 1 cut(s) 3353
Eco105I TACGTA 1 cut(s) 1488
Eco130I CCWWGG 4 cut(s) 92, 1075, 2231, 2852
Eco24I GRGCYC 1 cut(s) 1361
Eco31I GGTCTC 5 cut(s) 146, 504, 976, 2812, 3166
Eco32I GATATC 1 cut(s) 1535
Eco47I GGWCC 4 cut(s) 719, 1047, 2747, 3259
Eco57I CTGAAG 6 cut(s) 305, 1089, 1287, 2055, 2618, 2937
Eco88I CYCGRG 1 cut(s) 501
Eco91I GGTNACC 1 cut(s) 525
EcoNI CCTNNNNNAGG 2 cut(s) 648, 921
EcoO109I RGGNCCY 2 cut(s) 1047, 1357
EcoO65I GGTNACC 1 cut(s) 525
EcoRII CCWGG 5 cut(s) 156, 289, 334, 721, 2641
EcoRV GATATC 1 cut(s) 1535
EcoT14I CCWWGG 4 cut(s) 92, 1075, 2231, 2852
EcoT22I ATGCAT 2 cut(s) 1259, 1707
EcoT38I GRGCYC 1 cut(s) 1361
ErhI CCWWGG 4 cut(s) 92, 1075, 2231, 2852
FalI AAGNNNNNCTT 8 cut(s) 1453, 1485, 1500, 1532, 2499, 2531, 2836, 2868
FaqI GGGAC 5 cut(s) 1989, 2560, 2578, 3128, 3296
FauNDI CATATG 2 cut(s) 937, 1671
FbaI TGATCA 1 cut(s) 907
FblI GTMKAC 2 cut(s) 37, 587
FriOI GRGCYC 1 cut(s) 1361
GsuI CTGGAG 4 cut(s) 179, 318, 795, 1167
HaeIII GGCC 8 cut(s) 97, 726, 1184, 1359, 1625, 2678, 3371, 3484
HapII CCGG 7 cut(s) 64, 98, 220, 366, 450, 777, 2017
HgaI GACGC 2 cut(s) 23, 1393
Hin1I GRCGYC 2 cut(s) 108, 3141
HincII GTYRAC 4 cut(s) 656, 1330, 3091, 3139
HindII GTYRAC 4 cut(s) 656, 1330, 3091, 3139
HindIII AAGCTT 2 cut(s) 2114, 2862
HpaII CCGG 7 cut(s) 64, 98, 220, 366, 450, 777, 2017
Hpy99I CGWCG 3 cut(s) 253, 1278, 3389
HpyCH4IV ACGT 6 cut(s) 108, 1332, 1487, 2356, 2752, 3141
HpyF10VI GCNNNNNNNGC 9 cut(s) 28, 31, 94, 860, 1091, 1631, 2047, 3113, 3596
HpyF3I CTNAG 8 cut(s) 131, 1051, 1905, 2027, 2376, 2501, 2820, 3373
HpySE526I ACGT 6 cut(s) 108, 1332, 1487, 2356, 2752, 3141
Hsp92I GRCGYC 2 cut(s) 108, 3141
Ksp22I TGATCA 1 cut(s) 907
LguI GCTCTTC 1 cut(s) 2031
LmnI GCTCC 4 cut(s) 66, 442, 1392, 2103
LweI GCATC 5 cut(s) 1266, 1621, 3103, 3256, 3307
MaeII ACGT 6 cut(s) 108, 1332, 1487, 2356, 2752, 3141
MaeIII GTNAC 8 cut(s) 518, 525, 612, 1139, 1343, 1607, 1791, 3041
MfeI CAATTG 1 cut(s) 303
MflI RGATCY 3 cut(s) 1165, 2167, 2920
MhlI GDGCHC 2 cut(s) 1229, 1361
MlsI TGGCCA 1 cut(s) 726
MluNI TGGCCA 1 cut(s) 726
MlyI GAGTC 4 cut(s) 149, 2456, 3022, 3594
MmeI TCCRAC 2 cut(s) 2582, 2689
Mox20I TGGCCA 1 cut(s) 726
Mph1103I ATGCAT 2 cut(s) 1259, 1707
MroXI GAANNNNTTC 1 cut(s) 1512
MscI TGGCCA 1 cut(s) 726
MseI TTAA 8 cut(s) 56, 630, 1778, 2154, 2160, 2367, 2417, 2570
MslI CAYNNNNRTG 2 cut(s) 1323, 1884
Msp20I TGGCCA 1 cut(s) 726
MspA1I CMGCKG 3 cut(s) 121, 2552, 3085
MspI CCGG 7 cut(s) 64, 98, 220, 366, 450, 777, 2017
MspR9I CCNGG 9 cut(s) 158, 291, 336, 366, 450, 723, 777, 2018, 2643
MunI CAATTG 1 cut(s) 303
MvaI CCWGG 5 cut(s) 158, 291, 336, 723, 2643
MvnI CGCG 1 cut(s) 34
MwoI GCNNNNNNNGC 9 cut(s) 28, 31, 94, 860, 1091, 1631, 2047, 3113, 3596
NciI CCSGG 4 cut(s) 366, 450, 777, 2018
NcoI CCATGG 1 cut(s) 1075
NdeI CATATG 2 cut(s) 937, 1671
NlaIV GGNNCC 2 cut(s) 1359, 2286
NmeAIII GCCGAG 1 cut(s) 174
NmuCI GTSAC 4 cut(s) 518, 525, 612, 1791
NsiI ATGCAT 2 cut(s) 1259, 1707
NspI RCATGY 3 cut(s) 1313, 1709, 3515
PaeI GCATGC 1 cut(s) 1709
PaeR7I CTCGAG 1 cut(s) 501
PagI TCATGA 3 cut(s) 604, 2478, 3049
PaqCI CACCTGC 1 cut(s) 645
PciI ACATGT 1 cut(s) 1309
PciSI GCTCTTC 1 cut(s) 2031
PcsI WCGNNNNNNNCGW 1 cut(s) 677
PdmI GAANNNNTTC 1 cut(s) 1512
PflMI CCANNNNNTGG 1 cut(s) 3181
PleI GAGTC 4 cut(s) 148, 2455, 3022, 3593
PpsI GAGTC 4 cut(s) 148, 2455, 3022, 3593
Ppu21I YACGTR 1 cut(s) 1488
PpuMI RGGWCCY 1 cut(s) 1047
PscI ACATGT 1 cut(s) 1309
PshAI GACNNNNGTC 1 cut(s) 2611
PshBI ATTAAT 1 cut(s) 2417
PsiI TTATAA 1 cut(s) 3231
Psp5II RGGWCCY 1 cut(s) 1047
Psp6I CCWGG 5 cut(s) 156, 289, 334, 721, 2641
PspEI GGTNACC 1 cut(s) 525
PspGI CCWGG 5 cut(s) 156, 289, 334, 721, 2641
PspN4I GGNNCC 2 cut(s) 1359, 2286
PspOMI GGGCCC 1 cut(s) 1357
PspPI GGNCC 6 cut(s) 719, 1047, 1357, 1358, 2747, 3259
PspPPI RGGWCCY 1 cut(s) 1047
PsrI GAACNNNNNNTAC 2 cut(s) 3182, 3214
PstI CTGCAG 1 cut(s) 3084
PsuI RGATCY 3 cut(s) 1165, 2167, 2920
PvuII CAGCTG 3 cut(s) 121, 2552, 3085
RseI CAYNNNNRTG 2 cut(s) 1323, 1884
SapI GCTCTTC 1 cut(s) 2031
SaqAI TTAA 8 cut(s) 56, 630, 1778, 2154, 2160, 2367, 2417, 2570
Sau96I GGNCC 6 cut(s) 719, 1047, 1357, 1358, 2747, 3259
ScaI AGTACT 1 cut(s) 2224
SchI GAGTC 4 cut(s) 149, 2456, 3022, 3594
ScrFI CCNGG 9 cut(s) 158, 291, 336, 366, 450, 723, 777, 2018, 2643
SduI GDGCHC 2 cut(s) 1229, 1361
SfaNI GCATC 5 cut(s) 1266, 1621, 3103, 3256, 3307
SfcI CTRYAG 3 cut(s) 1391, 1855, 3080
Sfr274I CTCGAG 1 cut(s) 501
SinI GGWCC 4 cut(s) 719, 1047, 2747, 3259
SlaI CTCGAG 1 cut(s) 501
SmiMI CAYNNNNRTG 2 cut(s) 1323, 1884
SmlI CTYRAG 3 cut(s) 501, 875, 2809
SmoI CTYRAG 3 cut(s) 501, 875, 2809
SnaBI TACGTA 1 cut(s) 1488
SpeI ACTAGT 1 cut(s) 2626
SphI GCATGC 1 cut(s) 1709
SsiI CCGC 6 cut(s) 32, 125, 198, 2042, 2458, 3338
StyD4I CCNGG 9 cut(s) 156, 289, 334, 364, 448, 721, 775, 2016, 2641
StyI CCWWGG 4 cut(s) 92, 1075, 2231, 2852
TaiI ACGT 6 cut(s) 111, 1335, 1490, 2359, 2755, 3144
TaqI TCGA 9 cut(s) 418, 502, 1249, 1276, 1618, 1728, 1815, 2140, 3279
TatI WGTACW 6 cut(s) 481, 868, 1006, 2222, 3096, 3641
TauI GCSGC 3 cut(s) 34, 128, 2044
Tru1I TTAA 8 cut(s) 56, 630, 1778, 2154, 2160, 2367, 2417, 2570
Tru9I TTAA 8 cut(s) 56, 630, 1778, 2154, 2160, 2367, 2417, 2570
TscAI CASTG 7 cut(s) 819, 1270, 1955, 2063, 2086, 2178, 2199
TseFI GTSAC 4 cut(s) 518, 525, 612, 1791
Tsp45I GTSAC 4 cut(s) 518, 525, 612, 1791
TspGWI ACGGA 2 cut(s) 33, 237
TspRI CASTG 7 cut(s) 819, 1270, 1955, 2063, 2086, 2178, 2199
Van91I CCANNNNNTGG 1 cut(s) 3181
VpaK11BI GGWCC 4 cut(s) 719, 1047, 2747, 3259
VspI ATTAAT 1 cut(s) 2417
XagI CCTNNNNNAGG 2 cut(s) 648, 921
XapI RAATTY 1 cut(s) 2294
XbaI TCTAGA 3 cut(s) 766, 2440, 2923
XceI RCATGY 3 cut(s) 1313, 1709, 3515
XcmI CCANNNNNNNNNTGG 2 cut(s) 734, 2850
XhoI CTCGAG 1 cut(s) 501
XmaJI CCTAGG 1 cut(s) 2231
XmiI GTMKAC 2 cut(s) 37, 587
XmnI GAANNNNTTC 1 cut(s) 1512
ZraI GACGTC 2 cut(s) 109, 3142
ZrmI AGTACT 1 cut(s) 2224
Zsp2I ATGCAT 2 cut(s) 1259, 1707
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.