Probably involved in the RNA silencing pathway and required for the generation of small interfering RNAs (siRNAs)
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 232 |
| AccII | CGCG | 1 cut(s) | 308 |
| AccIII | TCCGGA | 1 cut(s) | 1530 |
| AciI | CCGC | 2 cut(s) | 308, 1273 |
| AclWI | GGATC | 8 cut(s) | 110, 125, 497, 764, 1170, 1183, 1390, 1631 |
| AcsI | RAATTY | 5 cut(s) | 145, 462, 968, 1199, 1230 |
| AcuI | CTGAAG | 1 cut(s) | 1034 |
| AfaI | GTAC | 4 cut(s) | 355, 584, 674, 1307 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 229, 809, 1073, 1405 |
| AjnI | CCWGG | 3 cut(s) | 592, 1493, 1603 |
| AjuI | GAANNNNNNNTTGG | 6 cut(s) | 642, 674, 792, 824, 841, 873 |
| AleI | CACNNNNGTG | 1 cut(s) | 606 |
| Alw21I | GWGCWC | 1 cut(s) | 885 |
| Alw26I | GTCTC | 2 cut(s) | 272, 726 |
| AlwI | GGATC | 8 cut(s) | 110, 125, 497, 764, 1170, 1183, 1390, 1631 |
| Aor13HI | TCCGGA | 1 cut(s) | 1530 |
| AoxI | GGCC | 2 cut(s) | 25, 627 |
| ApeKI | GCWGC | 4 cut(s) | 821, 938, 1364, 1565 |
| ApoI | RAATTY | 5 cut(s) | 145, 462, 968, 1199, 1230 |
| AseI | ATTAAT | 1 cut(s) | 1034 |
| AspLEI | GCGC | 2 cut(s) | 308, 1565 |
| AspS9I | GGNCC | 3 cut(s) | 698, 1270, 1588 |
| AsuHPI | GGTGA | 3 cut(s) | 620, 755, 1619 |
| AvaII | GGWCC | 3 cut(s) | 698, 1270, 1588 |
| AxyI | CCTNAGG | 1 cut(s) | 1266 |
| BaeGI | GKGCMC | 1 cut(s) | 1522 |
| BamHI | GGATCC | 1 cut(s) | 1175 |
| BanI | GGYRCC | 1 cut(s) | 232 |
| BanII | GRGCYC | 1 cut(s) | 885 |
| BauI | CACGAG | 1 cut(s) | 282 |
| BbsI | GAAGAC | 3 cut(s) | 908, 1218, 1260 |
| Bbv12I | GWGCWC | 1 cut(s) | 885 |
| BbvCI | CCTCAGC | 2 cut(s) | 771, 1410 |
| BbvI | GCAGC | 4 cut(s) | 808, 950, 1376, 1577 |
| BccI | CCATC | 4 cut(s) | 484, 734, 946, 955 |
| BceAI | ACGGC | 1 cut(s) | 12 |
| BciT130I | CCWGG | 3 cut(s) | 594, 1495, 1605 |
| BcoDI | GTCTC | 2 cut(s) | 272, 726 |
| BfaI | CTAG | 6 cut(s) | 545, 578, 647, 690, 906, 980 |
| BglII | AGATCT | 1 cut(s) | 1217 |
| BisI | GCNGC | 5 cut(s) | 309, 822, 939, 1365, 1566 |
| BlsI | GCNGC | 5 cut(s) | 310, 823, 940, 1366, 1567 |
| BmcAI | AGTACT | 1 cut(s) | 355 |
| Bme1390I | CCNGG | 3 cut(s) | 594, 1495, 1605 |
| Bme18I | GGWCC | 3 cut(s) | 698, 1270, 1588 |
| BmgT120I | GGNCC | 3 cut(s) | 698, 1270, 1588 |
| BmiI | GGNNCC | 2 cut(s) | 234, 1177 |
| BmrFI | CCNGG | 3 cut(s) | 594, 1495, 1605 |
| BmrI | ACTGGG | 1 cut(s) | 1524 |
| BmsI | GCATC | 1 cut(s) | 1051 |
| BmuI | ACTGGG | 1 cut(s) | 1524 |
| BpiI | GAAGAC | 3 cut(s) | 908, 1218, 1260 |
| BpmI | CTGGAG | 1 cut(s) | 173 |
| Bpu10I | CCTNAGC | 2 cut(s) | 771, 1410 |
| BpuEI | CTTGAG | 1 cut(s) | 745 |
| Bsa29I | ATCGAT | 1 cut(s) | 342 |
| BsaBI | GATNNNNATC | 1 cut(s) | 744 |
| BsaJI | CCNNGG | 3 cut(s) | 223, 803, 1329 |
| BsaWI | WCCGGW | 4 cut(s) | 585, 1308, 1530, 1590 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 229, 809, 1073, 1405 |
| Bse1I | ACTGG | 3 cut(s) | 156, 953, 1519 |
| Bse21I | CCTNAGG | 1 cut(s) | 1266 |
| Bse3DI | GCAATG | 1 cut(s) | 670 |
| Bse8I | GATNNNNATC | 1 cut(s) | 744 |
| BseAI | TCCGGA | 1 cut(s) | 1530 |
| BseBI | CCWGG | 3 cut(s) | 594, 1495, 1605 |
| BseCI | ATCGAT | 1 cut(s) | 342 |
| BseDI | CCNNGG | 3 cut(s) | 223, 803, 1329 |
| BseGI | GGATG | 3 cut(s) | 532, 871, 957 |
| BseJI | GATNNNNATC | 1 cut(s) | 744 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 229, 809, 1073, 1405 |
| BseMI | GCAATG | 1 cut(s) | 670 |
| BseMII | CTCAG | 3 cut(s) | 785, 1257, 1401 |
| BseNI | ACTGG | 3 cut(s) | 156, 953, 1519 |
| BseRI | GAGGAG | 1 cut(s) | 1427 |
| BseSI | GKGCMC | 1 cut(s) | 1522 |
| BseXI | GCAGC | 4 cut(s) | 808, 950, 1376, 1577 |
| Bsh1236I | CGCG | 1 cut(s) | 308 |
| BshFI | GGCC | 2 cut(s) | 27, 629 |
| BshNI | GGYRCC | 1 cut(s) | 232 |
| BshVI | ATCGAT | 1 cut(s) | 342 |
| BsiHKAI | GWGCWC | 1 cut(s) | 885 |
| BsiSI | CCGG | 4 cut(s) | 586, 1309, 1531, 1591 |
| BslFI | GGGAC | 2 cut(s) | 508, 1448 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 229, 809, 1073, 1405 |
| BsmAI | GTCTC | 2 cut(s) | 272, 726 |
| BsmFI | GGGAC | 2 cut(s) | 508, 1448 |
| BsmI | GAATGC | 1 cut(s) | 311 |
| BsnI | GGCC | 2 cut(s) | 27, 629 |
| Bsp1286I | GDGCHC | 2 cut(s) | 885, 1522 |
| Bsp13I | TCCGGA | 1 cut(s) | 1530 |
| Bsp19I | CCATGG | 2 cut(s) | 223, 1329 |
| BspACI | CCGC | 2 cut(s) | 308, 1273 |
| BspANI | GGCC | 2 cut(s) | 27, 629 |
| BspCNI | CTCAG | 3 cut(s) | 784, 1258, 1402 |
| BspDI | ATCGAT | 1 cut(s) | 342 |
| BspEI | TCCGGA | 1 cut(s) | 1530 |
| BspFNI | CGCG | 1 cut(s) | 308 |
| BspHI | TCATGA | 1 cut(s) | 1582 |
| BspLI | GGNNCC | 2 cut(s) | 234, 1177 |
| BspPI | GGATC | 8 cut(s) | 110, 125, 497, 764, 1170, 1183, 1390, 1631 |
| BspT107I | GGYRCC | 1 cut(s) | 232 |
| BsrDI | GCAATG | 1 cut(s) | 670 |
| BsrI | ACTGG | 3 cut(s) | 156, 953, 1519 |
| BssECI | CCNNGG | 3 cut(s) | 223, 803, 1329 |
| BssSI | CACGAG | 1 cut(s) | 282 |
| BssT1I | CCWWGG | 3 cut(s) | 223, 803, 1329 |
| Bst2BI | CACGAG | 1 cut(s) | 282 |
| Bst2UI | CCWGG | 3 cut(s) | 594, 1495, 1605 |
| Bst4CI | ACNGT | 4 cut(s) | 142, 358, 564, 677 |
| Bst6I | CTCTTC | 1 cut(s) | 1296 |
| BstC8I | GCNNGC | 2 cut(s) | 1561, 1577 |
| BstDEI | CTNAG | 4 cut(s) | 326, 771, 1266, 1410 |
| BstDSI | CCRYGG | 2 cut(s) | 223, 1329 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 1403 |
| BstF5I | GGATG | 3 cut(s) | 532, 871, 957 |
| BstFNI | CGCG | 1 cut(s) | 308 |
| BstHHI | GCGC | 2 cut(s) | 308, 1565 |
| BstMAI | GTCTC | 2 cut(s) | 272, 726 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 303, 1061, 1565 |
| BstNI | CCWGG | 3 cut(s) | 594, 1495, 1605 |
| BstSCI | CCNGG | 3 cut(s) | 592, 1493, 1603 |
| BstSLI | GKGCMC | 1 cut(s) | 1522 |
| BstUI | CGCG | 1 cut(s) | 308 |
| BstV1I | GCAGC | 4 cut(s) | 808, 950, 1376, 1577 |
| BstV2I | GAAGAC | 3 cut(s) | 908, 1218, 1260 |
| BstX2I | RGATCY | 7 cut(s) | 115, 130, 756, 1175, 1217, 1395, 1623 |
| BstYI | RGATCY | 7 cut(s) | 115, 130, 756, 1175, 1217, 1395, 1623 |
| Bsu15I | ATCGAT | 1 cut(s) | 342 |
| Bsu36I | CCTNAGG | 1 cut(s) | 1266 |
| BsuRI | GGCC | 2 cut(s) | 27, 629 |
| BsuTUI | ATCGAT | 1 cut(s) | 342 |
| BtgI | CCRYGG | 2 cut(s) | 223, 1329 |
| BtsCI | GGATG | 3 cut(s) | 532, 871, 957 |
| BtsI | GCAGTG | 1 cut(s) | 933 |
| BtsIMutI | CAGTG | 3 cut(s) | 147, 149, 933 |
| Cac8I | GCNNGC | 2 cut(s) | 1561, 1577 |
| CciI | TCATGA | 1 cut(s) | 1582 |
| CfoI | GCGC | 2 cut(s) | 308, 1565 |
| Cfr13I | GGNCC | 3 cut(s) | 698, 1270, 1588 |
| ClaI | ATCGAT | 1 cut(s) | 342 |
| CseI | GACGC | 1 cut(s) | 1466 |
| CsiI | ACCWGGT | 1 cut(s) | 1603 |
| Csp6I | GTAC | 4 cut(s) | 354, 583, 673, 1306 |
| CviAII | CATG | 8 cut(s) | 224, 433, 617, 836, 1129, 1284, 1330, 1583 |
| CviQI | GTAC | 4 cut(s) | 354, 583, 673, 1306 |
| DdeI | CTNAG | 4 cut(s) | 326, 771, 1266, 1410 |
| DraI | TTTAAA | 2 cut(s) | 273, 790 |
| Eam1104I | CTCTTC | 1 cut(s) | 1296 |
| EarI | CTCTTC | 1 cut(s) | 1296 |
| Ecl136II | GAGCTC | 1 cut(s) | 883 |
| Eco130I | CCWWGG | 3 cut(s) | 223, 803, 1329 |
| Eco24I | GRGCYC | 1 cut(s) | 885 |
| Eco47I | GGWCC | 3 cut(s) | 698, 1270, 1588 |
| Eco53kI | GAGCTC | 1 cut(s) | 883 |
| Eco57I | CTGAAG | 1 cut(s) | 1034 |
| Eco81I | CCTNAGG | 1 cut(s) | 1266 |
| EcoICRI | GAGCTC | 1 cut(s) | 883 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 1403 |
| EcoRI | GAATTC | 1 cut(s) | 145 |
| EcoRII | CCWGG | 3 cut(s) | 592, 1493, 1603 |
| EcoT14I | CCWWGG | 3 cut(s) | 223, 803, 1329 |
| EcoT38I | GRGCYC | 1 cut(s) | 885 |
| ErhI | CCWWGG | 3 cut(s) | 223, 803, 1329 |
| FaeI | CATG | 8 cut(s) | 227, 436, 620, 839, 1132, 1287, 1333, 1586 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 787, 819 |
| FaqI | GGGAC | 2 cut(s) | 508, 1448 |
| FatI | CATG | 8 cut(s) | 223, 432, 616, 835, 1128, 1283, 1329, 1582 |
| FauNDI | CATATG | 1 cut(s) | 400 |
| Fnu4HI | GCNGC | 5 cut(s) | 309, 822, 939, 1365, 1566 |
| FokI | GGATG | 3 cut(s) | 539, 878, 964 |
| FriOI | GRGCYC | 1 cut(s) | 885 |
| Fsp4HI | GCNGC | 5 cut(s) | 309, 822, 939, 1365, 1566 |
| FspBI | CTAG | 6 cut(s) | 545, 578, 647, 690, 906, 980 |
| GlaI | GCGC | 2 cut(s) | 307, 1564 |
| GluI | GCNGC | 5 cut(s) | 309, 822, 939, 1365, 1566 |
| GsuI | CTGGAG | 1 cut(s) | 173 |
| HaeIII | GGCC | 2 cut(s) | 27, 629 |
| HapII | CCGG | 4 cut(s) | 586, 1309, 1531, 1591 |
| HgaI | GACGC | 1 cut(s) | 1466 |
| HhaI | GCGC | 2 cut(s) | 308, 1565 |
| Hin1II | CATG | 8 cut(s) | 227, 436, 620, 839, 1132, 1287, 1333, 1586 |
| Hin6I | GCGC | 2 cut(s) | 306, 1563 |
| HinP1I | GCGC | 2 cut(s) | 306, 1563 |
| HindIII | AAGCTT | 2 cut(s) | 267, 919 |
| HinfI | GANTC | 8 cut(s) | 85, 280, 476, 589, 650, 976, 1148, 1357 |
| HpaII | CCGG | 4 cut(s) | 586, 1309, 1531, 1591 |
| HphI | GGTGA | 3 cut(s) | 620, 755, 1619 |
| Hpy166II | GTNNAC | 4 cut(s) | 583, 599, 701, 800 |
| Hpy188I | TCNGA | 4 cut(s) | 11, 130, 317, 1236 |
| Hpy8I | GTNNAC | 4 cut(s) | 583, 599, 701, 800 |
| Hpy99I | CGWCG | 2 cut(s) | 1262, 1460 |
| HpyAV | CCTTC | 3 cut(s) | 1009, 1099, 1636 |
| HpyCH4III | ACNGT | 4 cut(s) | 142, 358, 564, 677 |
| HpyCH4IV | ACGT | 4 cut(s) | 188, 703, 1260, 1476 |
| HpyCH4V | TGCA | 6 cut(s) | 4, 212, 406, 537, 1064, 1559 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 303, 1061, 1565 |
| HpyF3I | CTNAG | 4 cut(s) | 326, 771, 1266, 1410 |
| HpySE526I | ACGT | 4 cut(s) | 188, 703, 1260, 1476 |
| Hsp92II | CATG | 8 cut(s) | 227, 436, 620, 839, 1132, 1287, 1333, 1586 |
| HspAI | GCGC | 2 cut(s) | 306, 1563 |
| Kpn2I | TCCGGA | 1 cut(s) | 1530 |
| Lsp1109I | GCAGC | 4 cut(s) | 808, 950, 1376, 1577 |
| LweI | GCATC | 1 cut(s) | 1051 |
| MabI | ACCWGGT | 1 cut(s) | 1603 |
| MaeI | CTAG | 6 cut(s) | 545, 578, 647, 690, 906, 980 |
| MaeII | ACGT | 4 cut(s) | 188, 703, 1260, 1476 |
| MaeIII | GTNAC | 1 cut(s) | 255 |
| MflI | RGATCY | 7 cut(s) | 115, 130, 756, 1175, 1217, 1395, 1623 |
| MhlI | GDGCHC | 2 cut(s) | 885, 1522 |
| MluCI | AATT | 8 cut(s) | 122, 145, 462, 968, 1035, 1100, 1199, 1230 |
| MlyI | GAGTC | 2 cut(s) | 274, 970 |
| MmeI | TCCRAC | 2 cut(s) | 34, 530 |
| MroI | TCCGGA | 1 cut(s) | 1530 |
| MseI | TTAA | 6 cut(s) | 102, 177, 272, 779, 789, 1034 |
| MslI | CAYNNNNRTG | 1 cut(s) | 606 |
| MspA1I | CMGCKG | 2 cut(s) | 500, 821 |
| MspI | CCGG | 4 cut(s) | 586, 1309, 1531, 1591 |
| MspR9I | CCNGG | 3 cut(s) | 594, 1495, 1605 |
| MssI | GTTTAAAC | 1 cut(s) | 790 |
| Mva1269I | GAATGC | 1 cut(s) | 311 |
| MvaI | CCWGG | 3 cut(s) | 594, 1495, 1605 |
| MvnI | CGCG | 1 cut(s) | 308 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 303, 1061, 1565 |
| NcoI | CCATGG | 2 cut(s) | 223, 1329 |
| NdeI | CATATG | 1 cut(s) | 400 |
| NlaIII | CATG | 8 cut(s) | 227, 436, 620, 839, 1132, 1287, 1333, 1586 |
| NlaIV | GGNNCC | 2 cut(s) | 234, 1177 |
| OliI | CACNNNNGTG | 1 cut(s) | 606 |
| PagI | TCATGA | 1 cut(s) | 1582 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 194 |
| PctI | GAATGC | 1 cut(s) | 311 |
| PfeI | GAWTC | 6 cut(s) | 85, 476, 589, 650, 1148, 1357 |
| PkrI | GCNGC | 5 cut(s) | 310, 823, 940, 1366, 1567 |
| PleI | GAGTC | 2 cut(s) | 274, 970 |
| PmeI | GTTTAAAC | 1 cut(s) | 790 |
| PpsI | GAGTC | 2 cut(s) | 274, 970 |
| PshBI | ATTAAT | 1 cut(s) | 1034 |
| Psp124BI | GAGCTC | 1 cut(s) | 885 |
| Psp6I | CCWGG | 3 cut(s) | 592, 1493, 1603 |
| PspGI | CCWGG | 3 cut(s) | 592, 1493, 1603 |
| PspN4I | GGNNCC | 2 cut(s) | 234, 1177 |
| PspPI | GGNCC | 3 cut(s) | 698, 1270, 1588 |
| PsuI | RGATCY | 7 cut(s) | 115, 130, 756, 1175, 1217, 1395, 1623 |
| PvuII | CAGCTG | 2 cut(s) | 500, 821 |
| RsaI | GTAC | 4 cut(s) | 355, 584, 674, 1307 |
| RsaNI | GTAC | 4 cut(s) | 354, 583, 673, 1306 |
| RseI | CAYNNNNRTG | 1 cut(s) | 606 |
| SacI | GAGCTC | 1 cut(s) | 885 |
| SaqAI | TTAA | 6 cut(s) | 102, 177, 272, 779, 789, 1034 |
| SatI | GCNGC | 5 cut(s) | 309, 822, 939, 1365, 1566 |
| Sau96I | GGNCC | 3 cut(s) | 698, 1270, 1588 |
| ScaI | AGTACT | 1 cut(s) | 355 |
| SchI | GAGTC | 2 cut(s) | 274, 970 |
| ScrFI | CCNGG | 3 cut(s) | 594, 1495, 1605 |
| SduI | GDGCHC | 2 cut(s) | 885, 1522 |
| SexAI | ACCWGGT | 1 cut(s) | 1603 |
| SfaNI | GCATC | 1 cut(s) | 1051 |
| SinI | GGWCC | 3 cut(s) | 698, 1270, 1588 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 606 |
| SmlI | CTYRAG | 1 cut(s) | 760 |
| SmoI | CTYRAG | 1 cut(s) | 760 |
| Sse9I | AATT | 8 cut(s) | 122, 145, 462, 968, 1035, 1100, 1199, 1230 |
| SsiI | CCGC | 2 cut(s) | 308, 1273 |
| SspMI | CTAG | 6 cut(s) | 545, 578, 647, 690, 906, 980 |
| SstI | GAGCTC | 1 cut(s) | 885 |
| StyD4I | CCNGG | 3 cut(s) | 592, 1493, 1603 |
| StyI | CCWWGG | 3 cut(s) | 223, 803, 1329 |
| TaaI | ACNGT | 4 cut(s) | 142, 358, 564, 677 |
| TaiI | ACGT | 4 cut(s) | 191, 706, 1263, 1479 |
| TaqI | TCGA | 5 cut(s) | 88, 197, 252, 342, 1059 |
| TasI | AATT | 8 cut(s) | 122, 145, 462, 968, 1035, 1100, 1199, 1230 |
| TatI | WGTACW | 1 cut(s) | 353 |
| TauI | GCSGC | 1 cut(s) | 311 |
| TfiI | GAWTC | 6 cut(s) | 85, 476, 589, 650, 1148, 1357 |
| Tru1I | TTAA | 6 cut(s) | 102, 177, 272, 779, 789, 1034 |
| Tru9I | TTAA | 6 cut(s) | 102, 177, 272, 779, 789, 1034 |
| TscAI | CASTG | 3 cut(s) | 147, 156, 933 |
| TseI | GCWGC | 4 cut(s) | 821, 938, 1364, 1565 |
| TspGWI | ACGGA | 1 cut(s) | 697 |
| TspRI | CASTG | 3 cut(s) | 147, 156, 933 |
| VpaK11BI | GGWCC | 3 cut(s) | 698, 1270, 1588 |
| VspI | ATTAAT | 1 cut(s) | 1034 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 1403 |
| XapI | RAATTY | 5 cut(s) | 145, 462, 968, 1199, 1230 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 801, 833 |
| XspI | CTAG | 6 cut(s) | 545, 578, 647, 690, 906, 980 |
| ZrmI | AGTACT | 1 cut(s) | 355 |