Prupe.7G221200_v2.0.a1
ERF Family

Probably involved in the RNA silencing pathway and required for the generation of small interfering RNAs (siRNAs)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp07
OG0000505
Physical Location & Seq
Reverse (-)
19773819 .. 19783659
9841 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.7G221200.3

Sequence Viewer

Length: 3240 bp
ATGCATAATCACAGCAATTTGAACGTGAAACGCAGTCACCCACAGATGGAAAATGCCTCCAATCATTTATCTTCATCTATCAACCATTTTCACAGCAGCATGATCGAGCCCCAGACGGCCATGCCCGACGTTTGTCTACAGCCACAGCCACAACAGCAACCGCACATTGCTCTGCCTCCGTCGGTGGAGGATTTGCTGAACCAAATTTGTAACGGCAGAAAACAACCCCAGCCAGACCAAAACGTCAGACTGAGGCTGAGTTTTCTGAGCGAGGAGAAGGCCCTCCAACTCCTCCGTGAAATCGCCGAAGCGAAAACTATCAAAACTCTTAGTGGCTTAATCATTTGGATGATCCGTAATAAACTACAATATCAATGTGCCTCTCCTTCTCCCTCTCCTTCAAAGTCCGCCCCTGCTGCTTCTCTTCTACAAACCCAATTGTCTGCTTCTCCCATTACGCCTCCTGTTCATAAAGTAAGAATGGAAGTTACAGAGCAACCTGGAGACCATGGAGGGTTGAGGCCACCTTATTCCTATTCCTCTGCCTCACCTTCAAAAGTTCTCCGTGTTTATCCGTATCAAGGCCAATCATCTGCTCCTCCTTCAACTATTACTCCTGTTCGACTTTTTAAGGACCCTCTGCCTCTACCTCAACCTTTTCCTCAAGGTGAGACTTCCACTAATTCCTCAGTAGACCAACCTGGAGACAATGGGCAGGCACAATTAGAAGCTCTGGGCGAACTTGAGTTCAGGAGACAATTCCTAATATTGAATTACGCTGGAGGAAATAAGTTAGAAAAAGTTCTAGAGCCCGAGACTATTAGGAGTTGGAAAGATTTGCCGATGCAACTGTTTGAGACGACAGTTTGGGAAGCTTTGGGTCGGAACTATATCGGCACGCGACACCCGACTTTTGATTGGGATTCTGGAAAAACATACGTCTATCATTGTGAAGTCTCTGTGGATGGGAGCTACAAATTCAAGGGACCCTGTCTAAACAACACAAAAAGAACTCTCCTGCAGAAAGTTTTGGGAGATGACAATGTTTTAATGGTGAAATTTTCCGATGTGGTGACTGAGAGAGTTCCAACTGCCATTAAAGATAATAATTATGCCAATTATAGCAAGGTCGCAAGAGAAGGGATTCTTGTGGGTTTGCGTCGATATCAGTTCTTTGTGTTCAAAGATGGTGGCAATAAAGAAAAGAAGAAAAACCCAACTTCATCACCTGTGAAGTGTTACTTTATTTGTGTGGGCTCTAATGCCGCGATTGACAGGAGTGAAGATTATAAGTTCTCTAACAGAAAAATTCACGAAACAAGATGCATTTTTATGCATGCACATACTGTGTCTAGTGTGTCAAACTACATGGCTAGGTTTTCACTCATTTTGTCAAAGACAGAGAGCTTAGAAGTAGATTGGTCACTTGTAAAGGTTGAGGACATCGATGATGAGTATTGTCTAGATGAATCTGGTAATCGTATCGATAGAGATGGAAAGCCTCTTATACATACAGATGGAACTGGATTCATATCTGAGGATTTGGCTTTACTCTGTCCGAAGGATTTACTCAAAAGAGACTATATCAGTAAGGAATACATTGAGCCTTTGCTCTTGCAGTTCCGACTTTTTTACAAAGGCCGTGCTGTGAAGGGAACCTTTCTGATCAATAAAACGCTGCCACCAAAAACAATCCAGATTCGACCATCAATGGTTAAAGTTGAGACAGATCCAATGATTTCAGATGATCAAACAGTGAATTCGTTGGAGATAGTGACAGTAAGCAAATCACATAGGAATACATTTTTTTCGAGGCATTTAATTGCACTTCTATGTCATGGAGGGGTGCCGAAAGAGTACTTCAGGGAGTTACTTATGAAAGATCTGGAAGATACTCGTGGTGTTTTCTGCAGTAGGCGTGCAGCATTCAAAGTTGCCTATAACCACGGTGAGATCGACGATGATTACAATTCGGTAAAAATGATTCTTTCTGGTATTCCTCTTGAAGAATCATATTTGCAATATCGTCTATCAATTCTAAAGAAAGAGGAAAATAAGAGTCTTCAAAAAGGCAAAATTTGTAGTCCTCAGAGCTATATGTTGATGGGGACAGCTGATCCAACAGGGATTCTGGAAAGAGATGAAGTTTGCATTATCCTTGACAGCGGACAAATGTCGGGACAGGTACTAGTGTACCGGCATCCAGGTTTGCACTTTGGTGATATTCACCTTTTAAAGGCCAGATACGTGAAGGAGTTAGAATATGTTGTCGGCAATGCCAAATATGCTATATTCTTCTCTTGCAAAGGTCCACGTTCTGTAGCTGATGAAATGGGTGGTGGAGATTTTGACGGTGATCTCTATTGGGTCTCAAGAAACCCTCAGCTTTTGGAATGTTTTAAACCGAGTGAACCTTGGATCGAAGCTTCATCATCAACTCCAAAAGTTGCGAGTACTAGACCCAGTGAGCTCTTGCCTAATCATATAGAGGATGCGCTTATTAAACTATTCTTGAAAACTAGGTTTGAACCCAGTTTTGCAATGAGTGAGGCATCTGATAGCTGGCTGGCTATGATGGATCGGTTGTTAATTCTAGGAGATAGTAGTAATAGTGAGAAAACCCATGTGAAGGCAAACATGCTGCGGTTGGTTGATTTATACTACGAAGCTCTAGATGCACCAAAAAAAGGTGGAAAGGTGGTAGTTCCTGGAGAATTGAAGTCGAATCTGTTCCCTCATTACATGGAAAGGGTAAACTCATACAAATCAACGTCCATTTTGGGATTAATTTATGACACAGTGAACGCCTATCAAGCAGAAGATGCTTCTATCAAAGAAGTCAAGAAACTTCCTATGTTCGATGTCGAAGTCCCTGAGGAGTGCTTGAAGAAATGGAGGGAACACTACCAGCATTACAGGAGTGAAATGAGCTCGGCCATGCAGGACGATGATCGAGACAGCAAGAATAATGCTGCCGATAAGGTCTTGAGAAAGTATAAAGAGATTCTGTATGGTGGTGCTGAGGACCTTGAGAATAGTACAAGGCCGTTGCATGAAATATTCGACGAGGCGCTCGCAATATATCGGGTGACTTATGACCATGCTATAAGTCAAGGAGCTGTTGGTAAATGCTGCTTTGCATGGAAAGTTGCAGGTTCAGCCCTCTGCAAGTATTACATGAATAAACAAGGGGCCCGAACTATTGAAGCCTCATTCTCTGTTTTGAAGGACTTAGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RdRP

Protein Analysis

1080

Amino Acids

122.0

Weight (kDa)

7.15

Isoelectric Point (pI)

52.0

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000505)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G19910 AT2G19910 AT2G19920 AT2G19920 AT2G19920 AT2G19920 AT2G19930 AT2G19930 AT2G19930
fragaria_vesca FvH4_1g05980 FvH4_1g05990 FvH4_1g05990 FvH4_1g09718
malus_domestica MD02G1064400.v1.1 MD15G1195300.v1.1 MD15G1195400.v1.1
prunus_persica Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1
pyrus_communis pycom02g05070 pycom15g17460
rosa_chinensis RchiOBHm_Chr2g0091671 RchiOBHm_Chr2g0091681 RchiOBHm_Chr2g0091691 RchiOBHm_Chr2g0091701 RchiOBHm_Chr2g0091711 RchiOBHm_Chr2g0091721 RchiOBHm_Chr2g0091731 RchiOBHm_Chr5g0014401
rosa_laevigata RLG00000013772 RLG00000016242 RLG00000016244 RLG00000016245 RLG00000016246 RLG00000032105
rosa_multiflora Rmu_co8374041.1_g000001 Rmu_co8478121.1_g000001 Rmu_sc0000897.1_g000005 Rmu_sc0000897.1_g000009
rosa_roxburghii Rroxscaffold_1G00062180 Rroxscaffold_1G00062190 Rroxscaffold_1G00068800 Rroxscaffold_2G00149790 Rroxscaffold_2G00149800 Rroxscaffold_2G00149810
rosa_rugosa Rorug02G0019600 Rorug02G0019700 Rorug02G0019800 Rorug02G0019900 Rorug02G0019900 Rorug02G0020000 Rorug02G0020000 Rorug02G0020100 Rorug02G0020100 Rorug02G0020200 Rorug05G0017800
rosa_samantha Rh2AG064700 Rh2AG064800 Rh2AG064900 Rh2BG063800 Rh2BG063900 Rh2BG064000 Rh2BG064100 Rh2CG065500 Rh2CG065600 Rh2CG065700 Rh2DG063900 Rh2DG064000 Rh2DG064100 Rh2DG064200 Rh2DG381700 Rh5AG056700 Rh5AG056800 Rh5AG056900 Rh5AG112600 Rh5BG109300 Rh5CG063800 Rh5CG121100 Rh5DG053100 Rh5DG108300 Rh6CG100100
rosa_wichuraiana Rw2G005560 Rw2G005580 Rw2G005590 Rw5G005370 Rw5G009770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1290
AasI GACNNNNNNGTC 1 cut(s) 242
Acc36I ACCTGC 1 cut(s) 3143
AccB1I GGYRCC 1 cut(s) 1846
AccI GTMKAC 2 cut(s) 136, 693
AccII CGCG 2 cut(s) 901, 1268
AciI CCGC 5 cut(s) 161, 408, 1266, 2166, 2644
AclWI GGATC 5 cut(s) 346, 1724, 2111, 2426, 2586
AcoI YGGCCR 2 cut(s) 117, 2934
AcsI RAATTY 6 cut(s) 204, 977, 1058, 1308, 1759, 2076
AcuI CTGAAG 1 cut(s) 1846
AfaI GTAC 5 cut(s) 1859, 2187, 2195, 2455, 3040
AfiI CCNNNNNNNGG 5 cut(s) 46, 581, 2236, 2629, 2687
AhlI ACTAGT 1 cut(s) 2188
AjnI CCWGG 4 cut(s) 499, 700, 2203, 2707
AleI CACNNNNGTG 1 cut(s) 2217
Alw21I GWGCWC 2 cut(s) 2472, 2933
AlwI GGATC 5 cut(s) 346, 1724, 2111, 2426, 2586
Ama87I CYCGRG 1 cut(s) 812
AoxI GGCC 9 cut(s) 117, 279, 521, 583, 1639, 2238, 2934, 3044, 3192
ApaI GGGCCC 1 cut(s) 3196
ApeKI GCWGC 7 cut(s) 96, 416, 1678, 1922, 2641, 2972, 3132
ApoI RAATTY 6 cut(s) 204, 977, 1058, 1308, 1759, 2076
AseI ATTAAT 1 cut(s) 2786
Asp700I GAANNNNTTC 3 cut(s) 801, 1143, 2729
AspLEI GCGC 2 cut(s) 2497, 3073
AspS9I GGNCC 7 cut(s) 280, 634, 986, 2309, 3025, 3192, 3193
AvaI CYCGRG 1 cut(s) 812
AvaII GGWCC 4 cut(s) 634, 986, 2309, 3025
AxyI CCTNAGG 1 cut(s) 2874
BaeGI GKGCMC 1 cut(s) 3196
BaeI ACNNNNGTAYC 4 cut(s) 2177, 2177, 2210, 2210
BanI GGYRCC 1 cut(s) 1846
BanII GRGCYC 6 cut(s) 111, 813, 1259, 2472, 2933, 3196
BauI CACGAG 1 cut(s) 1896
BbsI GAAGAC 1 cut(s) 2054
Bbv12I GWGCWC 2 cut(s) 2472, 2933
BbvCI CCTCAGC 2 cut(s) 2382, 3021
BbvI GCAGC 7 cut(s) 108, 403, 1665, 1934, 2628, 2959, 3119
BccI CCATC 8 cut(s) 40, 959, 1181, 1487, 1511, 1714, 2098, 2569
BceAI ACGGC 4 cut(s) 132, 229, 1626, 3031
BcgI CGANNNNNNTGC 2 cut(s) 85, 119
BciT130I CCWGG 4 cut(s) 501, 702, 2205, 2709
BclI TGATCA 2 cut(s) 1665, 1747
BcuI ACTAGT 1 cut(s) 2188
BfaI CTAG 9 cut(s) 806, 1353, 1374, 1463, 2189, 2457, 2520, 2594, 2672
BfmI CTRYAG 4 cut(s) 137, 1019, 1909, 2319
BfoI RGCGCY 1 cut(s) 3074
BfuAI ACCTGC 1 cut(s) 3143
BglII AGATCT 1 cut(s) 1882
BisI GCNGC 8 cut(s) 97, 417, 1266, 1679, 1923, 2642, 2973, 3133
BlsI GCNGC 8 cut(s) 98, 418, 1267, 1680, 1924, 2643, 2974, 3134
BmcAI AGTACT 2 cut(s) 1859, 2455
Bme1390I CCNGG 4 cut(s) 501, 702, 2205, 2709
Bme18I GGWCC 4 cut(s) 634, 986, 2309, 3025
BmeT110I CYCGRG 1 cut(s) 812
BmgT120I GGNCC 7 cut(s) 280, 634, 986, 2309, 3025, 3192, 3193
BmiI GGNNCC 7 cut(s) 636, 987, 988, 1657, 1848, 3193, 3194
BmrFI CCNGG 4 cut(s) 501, 702, 2205, 2709
BmrI ACTGGG 2 cut(s) 2457, 2526
BmsI GCATC 7 cut(s) 834, 1313, 2209, 2482, 2561, 2665, 2812
BmuI ACTGGG 2 cut(s) 2457, 2526
BoxI GACNNNNGTC 2 cut(s) 132, 2173
BpiI GAAGAC 1 cut(s) 2054
BpmI CTGGAG 4 cut(s) 522, 723, 801, 2730
Bpu10I CCTNAGC 2 cut(s) 2382, 3021
BpuEI CTTGAG 5 cut(s) 648, 764, 2356, 3007, 3050
Bsa29I ATCGAT 2 cut(s) 1446, 1485
BsaAI YACGTR 1 cut(s) 2248
BsaBI GATNNNNATC 1 cut(s) 1531
BsaI GGTCTC 2 cut(s) 498, 2374
BsaJI CCNNGG 3 cut(s) 508, 1945, 2414
BsaXI ACNNNNNCTCC 2 cut(s) 598, 628
Bsc4I CCNNNNNNNGG 5 cut(s) 46, 581, 2236, 2629, 2687
Bse118I RCCGGY 1 cut(s) 2196
Bse1I ACTGG 3 cut(s) 1528, 2463, 2532
Bse21I CCTNAGG 1 cut(s) 2874
Bse3DI GCAATG 3 cut(s) 165, 2281, 2547
Bse8I GATNNNNATC 1 cut(s) 1531
BseBI CCWGG 4 cut(s) 501, 702, 2205, 2709
BseCI ATCGAT 2 cut(s) 1446, 1485
BseDI CCNNGG 3 cut(s) 508, 1945, 2414
BseGI GGATG 4 cut(s) 354, 970, 2200, 2497
BseJI GATNNNNATC 1 cut(s) 1531
BseLI CCNNNNNNNGG 5 cut(s) 46, 581, 2236, 2629, 2687
BseMI GCAATG 3 cut(s) 165, 2281, 2547
BseNI ACTGG 3 cut(s) 1528, 2463, 2532
BseRI GAGGAG 4 cut(s) 281, 287, 588, 2891
BseSI GKGCMC 1 cut(s) 3196
BseXI GCAGC 7 cut(s) 108, 403, 1665, 1934, 2628, 2959, 3119
BseYI CCCAGC 1 cut(s) 228
BsgI GTGCAG 1 cut(s) 1941
Bsh1236I CGCG 2 cut(s) 901, 1268
BshFI GGCC 9 cut(s) 119, 281, 523, 585, 1641, 2240, 2936, 3046, 3194
BshNI GGYRCC 1 cut(s) 1846
BshVI ATCGAT 2 cut(s) 1446, 1485
BsiHKAI GWGCWC 2 cut(s) 2472, 2933
BsiHKCI CYCGRG 1 cut(s) 812
BsiSI CCGG 1 cut(s) 2197
BslFI GGGAC 4 cut(s) 999, 2122, 2193, 2855
BslI CCNNNNNNNGG 5 cut(s) 46, 581, 2236, 2629, 2687
BsmBI CGTCTC 1 cut(s) 851
BsmFI GGGAC 4 cut(s) 999, 2122, 2193, 2855
BsmI GAATGC 1 cut(s) 1925
BsnI GGCC 9 cut(s) 119, 281, 523, 585, 1641, 2240, 2936, 3046, 3194
Bso31I GGTCTC 2 cut(s) 498, 2374
BsoBI CYCGRG 1 cut(s) 812
Bsp120I GGGCCC 1 cut(s) 3192
Bsp1286I GDGCHC 6 cut(s) 111, 813, 1259, 2472, 2933, 3196
Bsp19I CCATGG 1 cut(s) 508
BspACI CCGC 5 cut(s) 161, 408, 1266, 2166, 2644
BspANI GGCC 9 cut(s) 119, 281, 523, 585, 1641, 2240, 2936, 3046, 3194
BspDI ATCGAT 2 cut(s) 1446, 1485
BspFNI CGCG 2 cut(s) 901, 1268
BspLI GGNNCC 7 cut(s) 636, 987, 988, 1657, 1848, 3193, 3194
BspMAI CTGCAG 2 cut(s) 1023, 1913
BspMI ACCTGC 1 cut(s) 3143
BspPI GGATC 5 cut(s) 346, 1724, 2111, 2426, 2586
BspT107I GGYRCC 1 cut(s) 1846
BspTNI GGTCTC 2 cut(s) 498, 2374
BsrDI GCAATG 3 cut(s) 165, 2281, 2547
BsrFI RCCGGY 1 cut(s) 2196
BsrI ACTGG 3 cut(s) 1528, 2463, 2532
BssAI RCCGGY 1 cut(s) 2196
BssECI CCNNGG 3 cut(s) 508, 1945, 2414
BssSI CACGAG 1 cut(s) 1896
BssT1I CCWWGG 2 cut(s) 508, 2414
Bst2BI CACGAG 1 cut(s) 1896
Bst2UI CCWGG 4 cut(s) 501, 702, 2205, 2709
Bst4CI ACNGT 8 cut(s) 852, 865, 1348, 1756, 1780, 1949, 2354, 2800
Bst6I CTCTTC 1 cut(s) 429
BstAPI GCANNNNNTGC 1 cut(s) 2822
BstBAI YACGTR 1 cut(s) 2248
BstC8I GCNNGC 7 cut(s) 717, 899, 1338, 1920, 2564, 2568, 3075
BstDSI CCRYGG 2 cut(s) 508, 1945
BstENI CCTNNNNNAGG 1 cut(s) 2234
BstF5I GGATG 4 cut(s) 354, 970, 2200, 2497
BstFNI CGCG 2 cut(s) 901, 1268
BstH2I RGCGCY 1 cut(s) 3074
BstHHI GCGC 2 cut(s) 2497, 3073
BstMWI GCNNNNNNNGC 7 cut(s) 154, 416, 2285, 2675, 2813, 2822, 3158
BstNI CCWGG 4 cut(s) 501, 702, 2205, 2709
BstNSI RCATGY 2 cut(s) 1340, 2641
BstPAI GACNNNNGTC 2 cut(s) 132, 2173
BstSCI CCNGG 4 cut(s) 499, 700, 2203, 2707
BstSFI CTRYAG 4 cut(s) 137, 1019, 1909, 2319
BstSLI GKGCMC 1 cut(s) 3196
BstUI CGCG 2 cut(s) 901, 1268
BstV1I GCAGC 7 cut(s) 108, 403, 1665, 1934, 2628, 2959, 3119
BstV2I GAAGAC 1 cut(s) 2054
BstX2I RGATCY 2 cut(s) 1729, 1882
BstYI RGATCY 2 cut(s) 1729, 1882
Bsu15I ATCGAT 2 cut(s) 1446, 1485
Bsu36I CCTNAGG 1 cut(s) 2874
BsuRI GGCC 9 cut(s) 119, 281, 523, 585, 1641, 2240, 2936, 3046, 3194
BsuTUI ATCGAT 2 cut(s) 1446, 1485
BtgI CCRYGG 2 cut(s) 508, 1945
BtsCI GGATG 4 cut(s) 354, 970, 2200, 2497
BtsIMutI CAGTG 3 cut(s) 1761, 2470, 2805
BveI ACCTGC 1 cut(s) 3143
Cac8I GCNNGC 7 cut(s) 717, 899, 1338, 1920, 2564, 2568, 3075
CfoI GCGC 2 cut(s) 2497, 3073
Cfr10I RCCGGY 1 cut(s) 2196
Cfr13I GGNCC 7 cut(s) 280, 634, 986, 2309, 3025, 3192, 3193
ClaI ATCGAT 2 cut(s) 1446, 1485
CseI GACGC 1 cut(s) 1148
Csp6I GTAC 5 cut(s) 1858, 2186, 2194, 2454, 3039
CspCI CAANNNNNGTGG 2 cut(s) 1171, 1206
CviQI GTAC 5 cut(s) 1858, 2186, 2194, 2454, 3039
DraI TTTAAA 2 cut(s) 2235, 2401
DrdI GACNNNNNNGTC 1 cut(s) 242
DseDI GACNNNNNNGTC 1 cut(s) 242
EaeI YGGCCR 2 cut(s) 117, 2934
Eam1104I CTCTTC 1 cut(s) 429
EarI CTCTTC 1 cut(s) 429
EciI GGCGGA 1 cut(s) 397
Ecl136II GAGCTC 2 cut(s) 2470, 2931
Eco130I CCWWGG 2 cut(s) 508, 2414
Eco24I GRGCYC 6 cut(s) 111, 813, 1259, 2472, 2933, 3196
Eco31I GGTCTC 2 cut(s) 498, 2374
Eco32I GATATC 1 cut(s) 1166
Eco47I GGWCC 4 cut(s) 634, 986, 2309, 3025
Eco53kI GAGCTC 2 cut(s) 2470, 2931
Eco57I CTGAAG 1 cut(s) 1846
Eco81I CCTNAGG 1 cut(s) 2874
Eco88I CYCGRG 1 cut(s) 812
EcoICRI GAGCTC 2 cut(s) 2470, 2931
EcoNI CCTNNNNNAGG 1 cut(s) 2234
EcoO109I RGGNCCY 5 cut(s) 280, 634, 986, 3025, 3192
EcoRI GAATTC 1 cut(s) 1759
EcoRII CCWGG 4 cut(s) 499, 700, 2203, 2707
EcoRV GATATC 1 cut(s) 1166
EcoT14I CCWWGG 2 cut(s) 508, 2414
EcoT22I ATGCAT 3 cut(s) 6, 1328, 1338
EcoT38I GRGCYC 6 cut(s) 111, 813, 1259, 2472, 2933, 3196
ErhI CCWWGG 2 cut(s) 508, 2414
Esp3I CGTCTC 1 cut(s) 851
FalI AAGNNNNNCTT 6 cut(s) 1131, 1163, 1226, 1258, 1643, 1675
FaqI GGGAC 4 cut(s) 999, 2122, 2193, 2855
FbaI TGATCA 2 cut(s) 1665, 1747
FblI GTMKAC 2 cut(s) 136, 693
Fnu4HI GCNGC 8 cut(s) 97, 417, 1266, 1679, 1923, 2642, 2973, 3133
FokI GGATG 4 cut(s) 361, 977, 2187, 2504
FriOI GRGCYC 6 cut(s) 111, 813, 1259, 2472, 2933, 3196
Fsp4HI GCNGC 8 cut(s) 97, 417, 1266, 1679, 1923, 2642, 2973, 3133
FspBI CTAG 9 cut(s) 806, 1353, 1374, 1463, 2189, 2457, 2520, 2594, 2672
GlaI GCGC 2 cut(s) 2496, 3072
GluI GCNGC 8 cut(s) 97, 417, 1266, 1679, 1923, 2642, 2973, 3133
GsaI CCCAGC 1 cut(s) 232
GsuI CTGGAG 4 cut(s) 522, 723, 801, 2730
HaeII RGCGCY 1 cut(s) 3074
HaeIII GGCC 9 cut(s) 119, 281, 523, 585, 1641, 2240, 2936, 3046, 3194
HapII CCGG 1 cut(s) 2197
HgaI GACGC 1 cut(s) 1148
HhaI GCGC 2 cut(s) 2497, 3073
Hin6I GCGC 2 cut(s) 2495, 3071
HinP1I GCGC 2 cut(s) 2495, 3071
HindIII AAGCTT 2 cut(s) 873, 2424
HpaII CCGG 1 cut(s) 2197
Hpy166II GTNNAC 7 cut(s) 137, 694, 2194, 2312, 2411, 2755, 2803
Hpy8I GTNNAC 7 cut(s) 137, 694, 2194, 2312, 2411, 2755, 2803
Hpy99I CGWCG 5 cut(s) 131, 184, 1164, 1961, 3068
HpyCH4III ACNGT 8 cut(s) 852, 865, 1348, 1756, 1780, 1949, 2354, 2800
HpyCH4IV ACGT 7 cut(s) 24, 129, 243, 939, 2247, 2314, 2771
HpyF10VI GCNNNNNNNGC 7 cut(s) 154, 416, 2285, 2675, 2813, 2822, 3158
HpySE526I ACGT 7 cut(s) 24, 129, 243, 939, 2247, 2314, 2771
HspAI GCGC 2 cut(s) 2495, 3071
KflI GGGWCCC 1 cut(s) 986
Ksp22I TGATCA 2 cut(s) 1665, 1747
LmnI GCTCC 3 cut(s) 601, 969, 3116
Lsp1109I GCAGC 7 cut(s) 108, 403, 1665, 1934, 2628, 2959, 3119
LweI GCATC 7 cut(s) 834, 1313, 2209, 2482, 2561, 2665, 2812
MaeI CTAG 9 cut(s) 806, 1353, 1374, 1463, 2189, 2457, 2520, 2594, 2672
MaeII ACGT 7 cut(s) 24, 129, 243, 939, 2247, 2314, 2771
MaeIII GTNAC 9 cut(s) 35, 209, 487, 1072, 1238, 1422, 1774, 1869, 3088
MfeI CAATTG 1 cut(s) 437
MflI RGATCY 2 cut(s) 1729, 1882
MhlI GDGCHC 6 cut(s) 111, 813, 1259, 2472, 2933, 3196
MlyI GAGTC 1 cut(s) 2068
MmeI TCCRAC 7 cut(s) 310, 809, 863, 1112, 1648, 1746, 2144
Mph1103I ATGCAT 3 cut(s) 6, 1328, 1338
MroXI GAANNNNTTC 3 cut(s) 801, 1143, 2729
MslI CAYNNNNRTG 5 cut(s) 347, 1331, 1515, 1831, 2217
MspA1I CMGCKG 2 cut(s) 2114, 2166
MspI CCGG 1 cut(s) 2197
MspR9I CCNGG 4 cut(s) 501, 702, 2205, 2709
MunI CAATTG 1 cut(s) 437
Mva1269I GAATGC 1 cut(s) 1925
MvaI CCWGG 4 cut(s) 501, 702, 2205, 2709
MvnI CGCG 2 cut(s) 901, 1268
MwoI GCNNNNNNNGC 7 cut(s) 154, 416, 2285, 2675, 2813, 2822, 3158
NcoI CCATGG 1 cut(s) 508
NlaIV GGNNCC 7 cut(s) 636, 987, 988, 1657, 1848, 3193, 3194
NmeAIII GCCGAG 1 cut(s) 2912
NmuCI GTSAC 5 cut(s) 35, 1072, 1422, 1774, 3088
NsiI ATGCAT 3 cut(s) 6, 1328, 1338
NspI RCATGY 2 cut(s) 1340, 2641
OliI CACNNNNGTG 1 cut(s) 2217
PaeI GCATGC 1 cut(s) 1340
PcsI WCGNNNNNNNCGW 2 cut(s) 905, 1953
PctI GAATGC 1 cut(s) 1925
PdmI GAANNNNTTC 3 cut(s) 801, 1143, 2729
PflFI GACNNNGTC 1 cut(s) 990
PfoI TCCNGGA 1 cut(s) 2707
PkrI GCNGC 8 cut(s) 98, 418, 1267, 1680, 1924, 2643, 2974, 3134
PleI GAGTC 1 cut(s) 2067
PpsI GAGTC 1 cut(s) 2067
Ppu21I YACGTR 1 cut(s) 2248
PpuMI RGGWCCY 3 cut(s) 634, 986, 3025
PshAI GACNNNNGTC 2 cut(s) 132, 2173
PshBI ATTAAT 1 cut(s) 2786
PsiI TTATAA 1 cut(s) 1290
Psp124BI GAGCTC 2 cut(s) 2472, 2933
Psp5II RGGWCCY 3 cut(s) 634, 986, 3025
Psp6I CCWGG 4 cut(s) 499, 700, 2203, 2707
PspFI CCCAGC 1 cut(s) 228
PspGI CCWGG 4 cut(s) 499, 700, 2203, 2707
PspN4I GGNNCC 7 cut(s) 636, 987, 988, 1657, 1848, 3193, 3194
PspOMI GGGCCC 1 cut(s) 3192
PspPI GGNCC 7 cut(s) 280, 634, 986, 2309, 3025, 3192, 3193
PspPPI RGGWCCY 3 cut(s) 634, 986, 3025
PstI CTGCAG 2 cut(s) 1023, 1913
PsuI RGATCY 2 cut(s) 1729, 1882
PsyI GACNNNGTC 1 cut(s) 990
PvuII CAGCTG 1 cut(s) 2114
RsaI GTAC 5 cut(s) 1859, 2187, 2195, 2455, 3040
RsaNI GTAC 5 cut(s) 1858, 2186, 2194, 2454, 3039
RseI CAYNNNNRTG 5 cut(s) 347, 1331, 1515, 1831, 2217
SacI GAGCTC 2 cut(s) 2472, 2933
SatI GCNGC 8 cut(s) 97, 417, 1266, 1679, 1923, 2642, 2973, 3133
Sau96I GGNCC 7 cut(s) 280, 634, 986, 2309, 3025, 3192, 3193
ScaI AGTACT 2 cut(s) 1859, 2455
SchI GAGTC 1 cut(s) 2068
ScrFI CCNGG 4 cut(s) 501, 702, 2205, 2709
SduI GDGCHC 6 cut(s) 111, 813, 1259, 2472, 2933, 3196
SfaNI GCATC 7 cut(s) 834, 1313, 2209, 2482, 2561, 2665, 2812
SfcI CTRYAG 4 cut(s) 137, 1019, 1909, 2319
SinI GGWCC 4 cut(s) 634, 986, 2309, 3025
SmiMI CAYNNNNRTG 5 cut(s) 347, 1331, 1515, 1831, 2217
SmlI CTYRAG 5 cut(s) 663, 743, 2371, 2986, 3029
SmoI CTYRAG 5 cut(s) 663, 743, 2371, 2986, 3029
SpeI ACTAGT 1 cut(s) 2188
SphI GCATGC 1 cut(s) 1340
SsiI CCGC 5 cut(s) 161, 408, 1266, 2166, 2644
SspI AATATT 2 cut(s) 768, 3060
SspMI CTAG 9 cut(s) 806, 1353, 1374, 1463, 2189, 2457, 2520, 2594, 2672
SstI GAGCTC 2 cut(s) 2472, 2933
StyD4I CCNGG 4 cut(s) 499, 700, 2203, 2707
StyI CCWWGG 2 cut(s) 508, 2414
TaaI ACNGT 8 cut(s) 852, 865, 1348, 1756, 1780, 1949, 2354, 2800
TaiI ACGT 7 cut(s) 27, 132, 246, 942, 2250, 2317, 2774
TatI WGTACW 3 cut(s) 1857, 2453, 3038
TauI GCSGC 1 cut(s) 1268
TscAI CASTG 3 cut(s) 1761, 2470, 2805
TseFI GTSAC 5 cut(s) 35, 1072, 1422, 1774, 3088
TseI GCWGC 7 cut(s) 96, 416, 1678, 1922, 2641, 2972, 3132
Tsp45I GTSAC 5 cut(s) 35, 1072, 1422, 1774, 3088
TspGWI ACGGA 5 cut(s) 168, 284, 344, 554, 564
TspRI CASTG 3 cut(s) 1761, 2470, 2805
Tth111I GACNNNGTC 1 cut(s) 990
VpaK11BI GGWCC 4 cut(s) 634, 986, 2309, 3025
VspI ATTAAT 1 cut(s) 2786
XagI CCTNNNNNAGG 1 cut(s) 2234
XapI RAATTY 6 cut(s) 204, 977, 1058, 1308, 1759, 2076
XbaI TCTAGA 3 cut(s) 805, 1462, 2671
XceI RCATGY 2 cut(s) 1340, 2641
XmiI GTMKAC 2 cut(s) 136, 693
XmnI GAANNNNTTC 3 cut(s) 801, 1143, 2729
XspI CTAG 9 cut(s) 806, 1353, 1374, 1463, 2189, 2457, 2520, 2594, 2672
ZrmI AGTACT 2 cut(s) 1859, 2455
Zsp2I ATGCAT 3 cut(s) 6, 1328, 1338
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.