Rh2AG064700
ERF Family

Probably involved in the RNA silencing pathway and required for the generation of small interfering RNAs (siRNAs)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0000505
Physical Location & Seq
Forward (+)
5339622 .. 5340316
695 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG064700.1

Sequence Viewer

Length: 468 bp
ATGGAAGAAGTGAGATTACCTCCGCCGGTGGAGGAGTTGCTCCGTAGAATCTGTACTGAGAAAAAGCTACCATGGCCGGACGCCGGGGTCCGGCGGGAGCTGGCCTCTATCAGCCAGGAACGTGCACATGAGGTCCTGGTTAAAGTCTTCAACAAACAAAATATCATAACTCTCGGCGGTTTCATCAAGCACTTGATCCGTAACCTCCCTGCTTCTCCTCATCCTCCTCCGACAGCTGTCTGCGCTTATTATGATCAAAGCCCCTCTGCTTGTCCAGTTACGCCACCTTGTTCTCAACTTCAGTACAGCTCCGCAGTTGCAGGTCAGGCATTCAGTGATCGGACCGAGCAGCCTGTGATTAATGGACGGTCTGGACCATCACCATATTCTTCTGGTTGTAAAGCCATCAGCTCTTTCTCGTATCCAACAATACCGCTGGCTTGTAACATTGCTCTGGTTTGGTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RdRP

Protein Analysis

155

Amino Acids

16.97

Weight (kDa)

8.57

Isoelectric Point (pI)

85.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
4HB_RdRP3_N PF26249 4 - 67 8.1e-10 RdRP3/4/5, N-terminal four helical bundle domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000505)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G19910 AT2G19910 AT2G19920 AT2G19920 AT2G19920 AT2G19920 AT2G19930 AT2G19930 AT2G19930
fragaria_vesca FvH4_1g05980 FvH4_1g05990 FvH4_1g05990 FvH4_1g09718
malus_domestica MD02G1064400.v1.1 MD15G1195300.v1.1 MD15G1195400.v1.1
prunus_persica Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1
pyrus_communis pycom02g05070 pycom15g17460
rosa_chinensis RchiOBHm_Chr2g0091671 RchiOBHm_Chr2g0091681 RchiOBHm_Chr2g0091691 RchiOBHm_Chr2g0091701 RchiOBHm_Chr2g0091711 RchiOBHm_Chr2g0091721 RchiOBHm_Chr2g0091731 RchiOBHm_Chr5g0014401
rosa_laevigata RLG00000013772 RLG00000016242 RLG00000016244 RLG00000016245 RLG00000016246 RLG00000032105
rosa_multiflora Rmu_co8374041.1_g000001 Rmu_co8478121.1_g000001 Rmu_sc0000897.1_g000005 Rmu_sc0000897.1_g000009
rosa_roxburghii Rroxscaffold_1G00062180 Rroxscaffold_1G00062190 Rroxscaffold_1G00068800 Rroxscaffold_2G00149790 Rroxscaffold_2G00149800 Rroxscaffold_2G00149810
rosa_rugosa Rorug02G0019600 Rorug02G0019700 Rorug02G0019800 Rorug02G0019900 Rorug02G0019900 Rorug02G0020000 Rorug02G0020000 Rorug02G0020100 Rorug02G0020100 Rorug02G0020200 Rorug05G0017800
rosa_samantha Rh2AG064700 Rh2AG064800 Rh2AG064900 Rh2BG063800 Rh2BG063900 Rh2BG064000 Rh2BG064100 Rh2CG065500 Rh2CG065600 Rh2CG065700 Rh2DG063900 Rh2DG064000 Rh2DG064100 Rh2DG064200 Rh2DG381700 Rh5AG056700 Rh5AG056800 Rh5AG056900 Rh5AG112600 Rh5BG109300 Rh5CG063800 Rh5CG121100 Rh5DG053100 Rh5DG108300 Rh6CG100100
rosa_wichuraiana Rw2G005560 Rw2G005580 Rw2G005590 Rw5G005370 Rw5G009770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 86
Acc36I ACCTGC 1 cut(s) 311
AciI CCGC 5 cut(s) 23, 94, 177, 312, 434
AclWI GGATC 1 cut(s) 190
AcoI YGGCCR 1 cut(s) 74
AcuI CTGAAG 1 cut(s) 284
AcyI GRCGYC 1 cut(s) 81
AfaI GTAC 2 cut(s) 55, 305
AfiI CCNNNNNNNGG 2 cut(s) 83, 90
AgsI TTSAA 1 cut(s) 151
AjnI CCWGG 2 cut(s) 114, 135
AluBI AGCT 5 cut(s) 67, 100, 236, 309, 411
AluI AGCT 5 cut(s) 67, 100, 236, 309, 411
Alw21I GWGCWC 1 cut(s) 127
Alw44I GTGCAC 1 cut(s) 123
AlwI GGATC 1 cut(s) 190
AoxI GGCC 2 cut(s) 74, 102
ApaLI GTGCAC 1 cut(s) 123
ApeKI GCWGC 1 cut(s) 349
AseI ATTAAT 1 cut(s) 360
AspLEI GCGC 1 cut(s) 245
AspS9I GGNCC 4 cut(s) 88, 133, 342, 374
AsuC2I CCSGG 1 cut(s) 85
AsuHPI GGTGA 1 cut(s) 372
AvaII GGWCC 4 cut(s) 88, 133, 342, 374
BaeGI GKGCMC 1 cut(s) 127
BarI GAAGNNNNNNTAC 1 cut(s) 32
BbsI GAAGAC 1 cut(s) 139
Bbv12I GWGCWC 1 cut(s) 127
BbvI GCAGC 1 cut(s) 361
BccI CCATC 2 cut(s) 385, 413
BciT130I CCWGG 2 cut(s) 116, 137
BciVI GTATCC 1 cut(s) 432
BclI TGATCA 1 cut(s) 253
BcnI CCSGG 1 cut(s) 85
BfuAI ACCTGC 1 cut(s) 311
BfuI GTATCC 1 cut(s) 432
BisI GCNGC 1 cut(s) 350
BlsI GCNGC 1 cut(s) 351
Bme1390I CCNGG 3 cut(s) 85, 116, 137
Bme18I GGWCC 4 cut(s) 88, 133, 342, 374
BmgT120I GGNCC 4 cut(s) 88, 133, 342, 374
BmiI GGNNCC 1 cut(s) 89
BmrFI CCNGG 3 cut(s) 85, 116, 137
BoxI GACNNNNGTC 1 cut(s) 236
BpiI GAAGAC 1 cut(s) 139
BplI GAGNNNNNCTC 3 cut(s) 36, 89, 121
BpuMI CCSGG 1 cut(s) 85
BsaHI GRCGYC 1 cut(s) 81
BsaJI CCNNGG 2 cut(s) 71, 84
Bsc4I CCNNNNNNNGG 2 cut(s) 83, 90
Bse118I RCCGGY 1 cut(s) 25
Bse1I ACTGG 1 cut(s) 275
Bse3DI GCAATG 1 cut(s) 447
BseBI CCWGG 2 cut(s) 116, 137
BseDI CCNNGG 2 cut(s) 71, 84
BseGI GGATG 1 cut(s) 220
BseLI CCNNNNNNNGG 2 cut(s) 83, 90
BseMI GCAATG 1 cut(s) 447
BseMII CTCAG 1 cut(s) 48
BseNI ACTGG 1 cut(s) 275
BseRI GAGGAG 3 cut(s) 47, 207, 216
BseSI GKGCMC 1 cut(s) 127
BseXI GCAGC 1 cut(s) 361
BshFI GGCC 2 cut(s) 76, 104
BsiHKAI GWGCWC 1 cut(s) 127
BsiSI CCGG 4 cut(s) 26, 77, 84, 91
BslI CCNNNNNNNGG 2 cut(s) 83, 90
BsmI GAATGC 1 cut(s) 329
BsnI GGCC 2 cut(s) 76, 104
Bsp1286I GDGCHC 1 cut(s) 127
Bsp143I GATC 3 cut(s) 195, 253, 337
Bsp19I CCATGG 1 cut(s) 71
BspACI CCGC 5 cut(s) 23, 94, 177, 312, 434
BspANI GGCC 2 cut(s) 76, 104
BspCNI CTCAG 1 cut(s) 49
BspLI GGNNCC 1 cut(s) 89
BspMI ACCTGC 1 cut(s) 311
BspPI GGATC 1 cut(s) 190
BsrDI GCAATG 1 cut(s) 447
BsrFI RCCGGY 1 cut(s) 25
BsrI ACTGG 1 cut(s) 275
BssAI RCCGGY 1 cut(s) 25
BssECI CCNNGG 2 cut(s) 71, 84
BssMI GATC 3 cut(s) 195, 253, 337
BssNI GRCGYC 1 cut(s) 81
BssT1I CCWWGG 1 cut(s) 71
Bst2UI CCWGG 2 cut(s) 116, 137
Bst4CI ACNGT 1 cut(s) 369
BstACI GRCGYC 1 cut(s) 81
BstC8I GCNNGC 2 cut(s) 102, 438
BstDEI CTNAG 1 cut(s) 57
BstDSI CCRYGG 1 cut(s) 71
BstF5I GGATG 1 cut(s) 220
BstHHI GCGC 1 cut(s) 245
BstKTI GATC 3 cut(s) 198, 256, 340
BstMBI GATC 3 cut(s) 195, 253, 337
BstMWI GCNNNNNNNGC 3 cut(s) 73, 242, 326
BstNI CCWGG 2 cut(s) 116, 137
BstPAI GACNNNNGTC 1 cut(s) 236
BstSCI CCNGG 3 cut(s) 83, 114, 135
BstSLI GKGCMC 1 cut(s) 127
BstV1I GCAGC 1 cut(s) 361
BstV2I GAAGAC 1 cut(s) 139
BsuI GTATCC 1 cut(s) 432
BsuRI GGCC 2 cut(s) 76, 104
BtgI CCRYGG 1 cut(s) 71
BtsCI GGATG 1 cut(s) 220
BtsIMutI CAGTG 1 cut(s) 340
BveI ACCTGC 1 cut(s) 311
Cac8I GCNNGC 2 cut(s) 102, 438
CfoI GCGC 1 cut(s) 245
Cfr10I RCCGGY 1 cut(s) 25
Cfr13I GGNCC 4 cut(s) 88, 133, 342, 374
CpoI CGGWCCG 1 cut(s) 342
CseI GACGC 1 cut(s) 89
Csp6I GTAC 2 cut(s) 54, 304
CspI CGGWCCG 1 cut(s) 342
CviAII CATG 2 cut(s) 72, 128
CviQI GTAC 2 cut(s) 54, 304
DdeI CTNAG 1 cut(s) 57
DpnI GATC 3 cut(s) 197, 255, 339
DpnII GATC 3 cut(s) 195, 253, 337
DrdI GACNNNNNNGTC 1 cut(s) 86
DseDI GACNNNNNNGTC 1 cut(s) 86
EaeI YGGCCR 1 cut(s) 74
EciI GGCGGA 1 cut(s) 12
Eco130I CCWWGG 1 cut(s) 71
Eco47I GGWCC 4 cut(s) 88, 133, 342, 374
Eco57I CTGAAG 1 cut(s) 284
EcoO109I RGGNCCY 1 cut(s) 133
EcoRII CCWGG 2 cut(s) 114, 135
EcoT14I CCWWGG 1 cut(s) 71
ErhI CCWWGG 1 cut(s) 71
FaeI CATG 2 cut(s) 75, 131
FaiI YATR 5 cut(s) 73, 129, 167, 252, 385
FatI CATG 2 cut(s) 71, 127
FauI CCCGC 1 cut(s) 87
FbaI TGATCA 1 cut(s) 253
Fnu4HI GCNGC 1 cut(s) 350
FokI GGATG 1 cut(s) 207
Fsp4HI GCNGC 1 cut(s) 350
GlaI GCGC 1 cut(s) 244
GluI GCNGC 1 cut(s) 350
HaeIII GGCC 2 cut(s) 76, 104
HapII CCGG 4 cut(s) 26, 77, 84, 91
HgaI GACGC 1 cut(s) 89
HhaI GCGC 1 cut(s) 245
Hin1I GRCGYC 1 cut(s) 81
Hin1II CATG 2 cut(s) 75, 131
Hin6I GCGC 1 cut(s) 243
HinP1I GCGC 1 cut(s) 243
HinfI GANTC 1 cut(s) 48
HpaII CCGG 4 cut(s) 26, 77, 84, 91
HphI GGTGA 1 cut(s) 372
Hpy166II GTNNAC 1 cut(s) 125
Hpy188I TCNGA 2 cut(s) 231, 342
Hpy188III TCNNGA 1 cut(s) 372
Hpy8I GTNNAC 1 cut(s) 125
HpyCH4III ACNGT 1 cut(s) 369
HpyCH4IV ACGT 1 cut(s) 121
HpyCH4V TGCA 2 cut(s) 125, 320
HpyF10VI GCNNNNNNNGC 3 cut(s) 73, 242, 326
HpyF3I CTNAG 1 cut(s) 57
HpySE526I ACGT 1 cut(s) 121
Hsp92I GRCGYC 1 cut(s) 81
Hsp92II CATG 2 cut(s) 75, 131
HspAI GCGC 1 cut(s) 243
Ksp22I TGATCA 1 cut(s) 253
Kzo9I GATC 3 cut(s) 195, 253, 337
LmnI GCTCC 3 cut(s) 45, 97, 314
Lsp1109I GCAGC 1 cut(s) 361
MaeII ACGT 1 cut(s) 121
MaeIII GTNAC 3 cut(s) 200, 277, 443
MalI GATC 3 cut(s) 197, 255, 339
MboI GATC 3 cut(s) 195, 253, 337
MboII GAAGA 3 cut(s) 17, 139, 381
MhlI GDGCHC 1 cut(s) 127
MmeI TCCRAC 2 cut(s) 254, 449
MnlI CCTC 9 cut(s) 25, 30, 115, 124, 215, 228, 234, 237, 274
MseI TTAA 2 cut(s) 141, 360
MspA1I CMGCKG 2 cut(s) 236, 436
MspI CCGG 4 cut(s) 26, 77, 84, 91
MspR9I CCNGG 3 cut(s) 85, 116, 137
Mva1269I GAATGC 1 cut(s) 329
MvaI CCWGG 2 cut(s) 116, 137
MwoI GCNNNNNNNGC 3 cut(s) 73, 242, 326
NciI CCSGG 1 cut(s) 85
NcoI CCATGG 1 cut(s) 71
NdeII GATC 3 cut(s) 195, 253, 337
NlaIII CATG 2 cut(s) 75, 131
NlaIV GGNNCC 1 cut(s) 89
NmeAIII GCCGAG 1 cut(s) 153
PctI GAATGC 1 cut(s) 329
PfeI GAWTC 1 cut(s) 48
PkrI GCNGC 1 cut(s) 351
PpuMI RGGWCCY 1 cut(s) 133
PshAI GACNNNNGTC 1 cut(s) 236
PshBI ATTAAT 1 cut(s) 360
Psp5II RGGWCCY 1 cut(s) 133
Psp6I CCWGG 2 cut(s) 114, 135
PspGI CCWGG 2 cut(s) 114, 135
PspN4I GGNNCC 1 cut(s) 89
PspPI GGNCC 4 cut(s) 88, 133, 342, 374
PspPPI RGGWCCY 1 cut(s) 133
PvuII CAGCTG 1 cut(s) 236
RsaI GTAC 2 cut(s) 55, 305
RsaNI GTAC 2 cut(s) 54, 304
Rsr2I CGGWCCG 1 cut(s) 342
RsrII CGGWCCG 1 cut(s) 342
SaqAI TTAA 2 cut(s) 141, 360
SatI GCNGC 1 cut(s) 350
Sau3AI GATC 3 cut(s) 195, 253, 337
Sau96I GGNCC 4 cut(s) 88, 133, 342, 374
ScrFI CCNGG 3 cut(s) 85, 116, 137
SduI GDGCHC 1 cut(s) 127
SgrAI CRCCGGYG 1 cut(s) 25
SinI GGWCC 4 cut(s) 88, 133, 342, 374
SsiI CCGC 5 cut(s) 23, 94, 177, 312, 434
StyD4I CCNGG 3 cut(s) 83, 114, 135
StyI CCWWGG 1 cut(s) 71
TaaI ACNGT 1 cut(s) 369
TaiI ACGT 1 cut(s) 124
TaqII GACCGA 1 cut(s) 359
TatI WGTACW 2 cut(s) 53, 303
TfiI GAWTC 1 cut(s) 48
Tru1I TTAA 2 cut(s) 141, 360
Tru9I TTAA 2 cut(s) 141, 360
TscAI CASTG 1 cut(s) 340
TseI GCWGC 1 cut(s) 349
TspDTI ATGAA 1 cut(s) 172
TspGWI ACGGA 2 cut(s) 32, 188
TspRI CASTG 1 cut(s) 340
VneI GTGCAC 1 cut(s) 123
VpaK11BI GGWCC 4 cut(s) 88, 133, 342, 374
VspI ATTAAT 1 cut(s) 360
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.