Rh5DG108300
ERF Family

Probably involved in the RNA silencing pathway and required for the generation of small interfering RNAs (siRNAs)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0000505
Physical Location & Seq
Reverse (-)
9840584 .. 9851074
10491 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG108300.1

Sequence Viewer

Length: 3423 bp
ATGTTCGGCGTGCAGCTACTCGACTCCGTGCAGCAATCGCTGGATCGAATATGTCACCTTAAGAAGCAACCGCAGCCGGACGACGGCATCAGAAGAAAGCTGGCATTGCTGGGCGAGCAAGACGCCCTCTACGTGCTGGACCAAATTTACAACAGCAAAACTGAGATCAGAACCCTCAATGGATTCATTGCTTTCATGATCAGGAAACTAGAGAATGAATCTTCTTCGTCTCCTTCTTCTAGAACAGTTTGGACCCCTCAGAGTCCCTCTGCTTCTTCTTCTTCTTCTTCTGTTACTGGCCCTGCACAGCTTTATCACAGCCCTGATCATTCTCAAGTTGTAGACCAGTCTGAAGAGAGTGGACTCTGTTCCTCTGTTTTTCAGCGGCTATATTTGAGCTCTCAAGGTGAGACAATTCCTTGCGGTTCTCCTGGTGTAGAGCAACATCAAGACTATAATCGACAGCCACCCTATTCTTCAGCTTCTCCTTCTGAAGCTTTCCAGACTTCTTATTATCAGAGCCATTCTTCTCCTACTAGTGAGACTCCCAACCCATCCCCGATGACAGTGTGCAATGGCCAATCACCTTATTCCTCCTCTTCTCCTTATCAAATCCTGTCTGCTCCTTCTAATACTCATAGACAACTCTATGAGAGGTTCCAAGGTGTCCATGTTTCTCCTTTTCAAACCCTATCTGCCTCTCCTGTTACTCCTGTTCAACTTTATAAGAGCTTTCAAGATGAGACTGTCACTCACTCCCCAGTTCTATACCATCCTGGTGACCATGGACCAGCACACTTAGAAGCTCTGGGAGAACTTGAGTTCAGAAGACAATTTCTAATATTAAGTTATGCTGGAGGAATGAAGTTAAAAGATGTAATAGAAGTGGAGGCTATTAGGCGCTGGAGAAATTTGCCAATGGTAAACTTCGAGACCAAAGTTTGGCATGCTTTGGGTGAAAACAATGTTGGCAAGGCTGACCGACGATTGTATTCTGATTGGGATTCTCAAAGAACACACCACTACTATTGTTATGTTTCCGTGGATGGGAGTTGCAAGTTCAAGGGTCCCTTCCTAGACAAACCAAGAACTCTCCTACAGAAATGGTTAGAAGATGACAATGTTTTAGAGGTGAAATTTACCCAAGGGGAAGGTTCCAGTATTCTTTTTGATAATAATTATGGTACATATAGCAAGTTAATGCGAGAAGGCATTCTTGTCGGTTTGCGGCGATATGGTCACTTTGTGCACAGTGTTGGTGGTAACAAAGGAAAGAATAAGTTGAAAGACCCAACTTCATCACCTGTGAAATGTTACTTTGTTCGTATGAGCTCTGATGCAGCGATAGACATGGGTAAATTTTATAAGCTAGCTGGCAAAACGATGCATGAAGGAAGATCTCTTTTGACGCACGTACACACTGGGCCTAGTTTGGCTAATTACATGGCTAGGTTATCTCTTATTTTGTCCAAGACAGTTACTTTAGAAGTGAATTGGTCACTTGTCAAGGTTGAGTTCATCAATGAAGAATTCTGTCTCGATGAGTCTGGTAATCCCATCTATGTAGATGGAAAAGCTTGTATACATACTGATGGCACGGGATTTATATCTGCGGATCTGGCTTTACTTTGTCCTGAGATAGCACACAAAGGAGAGCTTATCAATAATGAACACATCGAGGGACTTGATGATCCTGATGATGAACTTGAGGACAACATTCTGGAATGGAAACAGCCAGGCACTAGAACTCAGGAACTGCCTTTGCTCATGCAGTTCCGACTTTTTTACAAAGGCTATGCTATCAAAGGAACTTTTCTTGTCAATAAACTGCTTCCACCAAACACATTGCAGATTCGACCGTCAATGGTCAAAGTTGAGCCAGATCCAAAACTGTCAGATCCTCAAACAGTGAATTCACTGGAGATAGTAGGAACAAGTAAACTTACTAAGAGAACAAATTTGTCGAGGAACTTGATTGCACTTTTATACCATGGAGGGGTGCCAAAAGAATACTTCATCGAGTTACTTCTGAAAGCTTTAGAAGAGACTCGTGACGTTTATAGCAATAAACGTGCAGCATTCAGAGTTGCCCTTAGATATGGCGAGATCGACGACAATAGTAATGTTGCGAAAATGATCTCCTGTGGTATTCCTCTTGAAGAACCATATTTGCAATATCGTCTTTCAGTCCTTATGAACATGGAAAAGATCAATCTTAAAAAAGGGAAAATTTGTATTCCTGAATCATACTATTTGATGGGGACAGCTGATCCAACAGGAAAACTTGAAAAAGATCAAGTTTGCATTATCCTAAAAGATGGACAACTGTCGGGAATGGTTCTAGTGTACCGGAATCCTGGTGTACACTTCGGTGATATTCATGTCCTAAAAGCCACGTATGTCAAGGAACTAGAATCTGTTGTTGGAAATGCCAAGTACGGTATATTCTTCTCTTGTAAAGGTCCACGTTCCGTTGCGGATGAAATGGGTGGTGGAGATTTTGATGGTGATCTATACTGGATCTCAAGAAACCCTCAGCTGTTGGAATCGTTTAAACCAAGTGAACCTTGGATTGAAAAGTCATCTACCCAAAAAGTTCATGGTAAAAGACCAACCGAGCTTTCAAATGTTGATCTAGAAGATGAGCTCATCAAACTATTCTTGAAGACTAGGTTTGAACCAAGCTTCGCAATGAGTGAGGCAGCTCAACACTGGATGGCTTGGATGGACAAATTTTTGAGTTTAGCAGATACGAGTATTGATGAGAAAAATGAACTGAAGGCAAAGATACTGCAATTGATTGATATTTACTATGAAGCTCTCGATGCACCCAAGAAAGGTTTTAAGGTTGTAGTTCCAAAAGAATTGAAGGTGAAGACGTTTCCACATTTCATGGAGAAGAAACCCCTAGATTCCTACCATTCAATTTCCATTTTGGGATCAATTTATGATAAGGTGGATGAATATGAAACAGAAGACAGATCTCTCAAAGAAATTCAGAAACTTCCTCTGTTTGAAGTTGACGTTCCTGAGGGTAGCTTGAAAGAATGGAAGAAACTATACCAAGATTACCGGAATGAGATGACCTCTGCCATGGGAGCCGAAGATAAACAGGGCCAGAATCAGGCAGCCAATATCGTCATAAGAAAGTATAAAGAGATCCTATACAAGGCTGAGGAGTTTGAGCAAAGTGATATGCGGCACAGACAACAAATATATAATGACGCACTCGCTATTTATCACGTTTGTTATGACTATGCCAAGAGTGTTAGGACTGAAACGGGTGCACAAGATGTCAAGAAATGTGGCTTTGCTTGGAAAGTTGCAGGTGCAGCTCTATGCCAGCTTCACGAAGACTGGAGTTTGAAACCTGGTGAAAAGGTTTTCAGAGTCTTACCCACTGTTTTGAAGGACATTATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RdRP

Protein Analysis

1140

Amino Acids

129.06

Weight (kDa)

6.23

Isoelectric Point (pI)

45.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
4HB_RdRP3_N PF26249 6 - 69 2.8e-07 RdRP3/4/5, N-terminal four helical bundle domain
RdRP_helical PF26252 266 - 330 4.4e-06 RNA dependent RNA polymerase, helical domain
RdRP PF05183 353 - 986 3.4e-99 RNA dependent RNA polymerase
RdRP_head PF26253 1014 - 1118 3.6e-09 RdRP, head domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000505)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G19910 AT2G19910 AT2G19920 AT2G19920 AT2G19920 AT2G19920 AT2G19930 AT2G19930 AT2G19930
fragaria_vesca FvH4_1g05980 FvH4_1g05990 FvH4_1g05990 FvH4_1g09718
malus_domestica MD02G1064400.v1.1 MD15G1195300.v1.1 MD15G1195400.v1.1
prunus_persica Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1
pyrus_communis pycom02g05070 pycom15g17460
rosa_chinensis RchiOBHm_Chr2g0091671 RchiOBHm_Chr2g0091681 RchiOBHm_Chr2g0091691 RchiOBHm_Chr2g0091701 RchiOBHm_Chr2g0091711 RchiOBHm_Chr2g0091721 RchiOBHm_Chr2g0091731 RchiOBHm_Chr5g0014401
rosa_laevigata RLG00000013772 RLG00000016242 RLG00000016244 RLG00000016245 RLG00000016246 RLG00000032105
rosa_multiflora Rmu_co8374041.1_g000001 Rmu_co8478121.1_g000001 Rmu_sc0000897.1_g000005 Rmu_sc0000897.1_g000009
rosa_roxburghii Rroxscaffold_1G00062180 Rroxscaffold_1G00062190 Rroxscaffold_1G00068800 Rroxscaffold_2G00149790 Rroxscaffold_2G00149800 Rroxscaffold_2G00149810
rosa_rugosa Rorug02G0019600 Rorug02G0019700 Rorug02G0019800 Rorug02G0019900 Rorug02G0019900 Rorug02G0020000 Rorug02G0020000 Rorug02G0020100 Rorug02G0020100 Rorug02G0020200 Rorug05G0017800
rosa_samantha Rh2AG064700 Rh2AG064800 Rh2AG064900 Rh2BG063800 Rh2BG063900 Rh2BG064000 Rh2BG064100 Rh2CG065500 Rh2CG065600 Rh2CG065700 Rh2DG063900 Rh2DG064000 Rh2DG064100 Rh2DG064200 Rh2DG381700 Rh5AG056700 Rh5AG056800 Rh5AG056900 Rh5AG112600 Rh5BG109300 Rh5CG063800 Rh5CG121100 Rh5DG053100 Rh5DG108300 Rh6CG100100
rosa_wichuraiana Rw2G005560 Rw2G005580 Rw2G005590 Rw5G005370 Rw5G009770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 726, 1365
AarI CACCTGC 1 cut(s) 3317
Acc36I ACCTGC 1 cut(s) 3317
AccB1I GGYRCC 1 cut(s) 1999
AccB7I CCANNNNNTGG 1 cut(s) 942
AccI GTMKAC 2 cut(s) 342, 1582
AciI CCGC 7 cut(s) 71, 385, 423, 1228, 1613, 2477, 3199
AclWI GGATC 9 cut(s) 51, 1623, 1685, 1877, 1892, 2264, 2528, 2947, 3154
AcoI YGGCCR 1 cut(s) 577
AcuI CTGAAG 4 cut(s) 372, 462, 513, 2798
AcyI GRCGYC 1 cut(s) 123
AdeI CACNNNGTG 1 cut(s) 1246
AfaI GTAC 5 cut(s) 1186, 1416, 2348, 2364, 2438
AfiI CCNNNNNNNGG 7 cut(s) 83, 942, 1047, 1996, 2837, 3124, 3169
AflII CTTAAG 1 cut(s) 59
AhlI ACTAGT 1 cut(s) 536
AjnI CCWGG 5 cut(s) 430, 775, 1735, 2356, 3370
AjuI GAANNNNNNNTTGG 6 cut(s) 951, 983, 2406, 2438, 2605, 2637
AleI CACNNNNGTG 1 cut(s) 2370
Alw21I GWGCWC 5 cut(s) 401, 1251, 1334, 2649, 3289
Alw26I GTCTC 7 cut(s) 234, 404, 536, 737, 926, 1541, 2039
Alw44I GTGCAC 2 cut(s) 1247, 3285
AlwI GGATC 9 cut(s) 51, 1623, 1685, 1877, 1892, 2264, 2528, 2947, 3154
AlwNI CAGNNNCTG 1 cut(s) 1756
AoxI GGCC 4 cut(s) 298, 577, 1424, 3115
ApaLI GTGCAC 2 cut(s) 1247, 3285
ApeKI GCWGC 8 cut(s) 13, 31, 73, 1340, 2075, 2702, 3128, 3332
Asp700I GAANNNNTTC 1 cut(s) 1212
AspLEI GCGC 1 cut(s) 903
AspS9I GGNCC 8 cut(s) 139, 252, 299, 788, 1067, 1424, 2462, 3115
AsuNHI GCTAGC 1 cut(s) 1369
AvaII GGWCC 5 cut(s) 139, 252, 788, 1067, 2462
AxyI CCTNAGG 1 cut(s) 3030
BaeGI GKGCMC 2 cut(s) 1251, 3289
BalI TGGCCA 1 cut(s) 579
BanI GGYRCC 1 cut(s) 1999
BanII GRGCYC 3 cut(s) 401, 1334, 2649
BarI GAAGNNNNNNTAC 2 cut(s) 3044, 3076
BauI CACGAG 1 cut(s) 2049
BbsI GAAGAC 5 cut(s) 835, 2672, 2882, 2982, 3360
Bbv12I GWGCWC 5 cut(s) 401, 1251, 1334, 2649, 3289
BbvCI CCTCAGC 2 cut(s) 2535, 3174
BbvI GCAGC 8 cut(s) 25, 43, 85, 1352, 2087, 2714, 3140, 3344
BceAI ACGGC 1 cut(s) 100
BcgI CGANNNNNNTGC 2 cut(s) 1201, 1235
BciT130I CCWGG 5 cut(s) 432, 777, 1737, 2358, 3372
BclI TGATCA 2 cut(s) 198, 325
BcoDI GTCTC 7 cut(s) 234, 404, 536, 737, 926, 1541, 2039
BcuI ACTAGT 1 cut(s) 536
BfmI CTRYAG 1 cut(s) 1097
BfoI RGCGCY 1 cut(s) 904
BfrI CTTAAG 1 cut(s) 59
BfuAI ACCTGC 1 cut(s) 3317
BglII AGATCT 2 cut(s) 1397, 2981
Bme1390I CCNGG 5 cut(s) 432, 777, 1737, 2358, 3372
Bme18I GGWCC 5 cut(s) 139, 252, 788, 1067, 2462
BmgT120I GGNCC 8 cut(s) 139, 252, 299, 788, 1067, 1424, 2462, 3115
BmiI GGNNCC 7 cut(s) 254, 659, 1068, 1069, 1156, 2001, 3100
BmrFI CCNGG 5 cut(s) 432, 777, 1737, 2358, 3372
BmrI ACTGGG 2 cut(s) 755, 1431
BmsI GCATC 4 cut(s) 96, 1327, 1374, 2815
BmtI GCTAGC 1 cut(s) 1373
BmuI ACTGGG 2 cut(s) 755, 1431
BoxI GACNNNNGTC 1 cut(s) 2326
BpiI GAAGAC 5 cut(s) 835, 2672, 2882, 2982, 3360
BplI GAGNNNNNCTC 2 cut(s) 3071, 3103
BpmI CTGGAG 4 cut(s) 876, 925, 1940, 3379
Bpu10I CCTNAGC 2 cut(s) 2535, 3174
BpuEI CTTGAG 5 cut(s) 318, 387, 839, 1727, 2509
BsaAI YACGTR 3 cut(s) 133, 1414, 2397
BsaBI GATNNNNATC 2 cut(s) 1606, 2508
BsaHI GRCGYC 1 cut(s) 123
BsaI GGTCTC 1 cut(s) 926
BsaJI CCNNGG 7 cut(s) 661, 784, 1041, 1144, 1990, 2567, 3093
BsaWI WCCGGW 2 cut(s) 2349, 3072
Bsc4I CCNNNNNNNGG 7 cut(s) 83, 942, 1047, 1996, 2837, 3124, 3169
Bse1I ACTGG 9 cut(s) 301, 346, 761, 1158, 1426, 1923, 2522, 2717, 3362
Bse21I CCTNAGG 1 cut(s) 3030
Bse3DI GCAATG 5 cut(s) 104, 186, 580, 1844, 2697
Bse8I GATNNNNATC 2 cut(s) 1606, 2508
BseBI CCWGG 5 cut(s) 432, 777, 1737, 2358, 3372
BseDI CCNNGG 7 cut(s) 661, 784, 1041, 1144, 1990, 2567, 3093
BseGI GGATG 7 cut(s) 554, 772, 1051, 2485, 2721, 2730, 2965
BseJI GATNNNNATC 2 cut(s) 1606, 2508
BseLI CCNNNNNNNGG 7 cut(s) 83, 942, 1047, 1996, 2837, 3124, 3169
BseMI GCAATG 5 cut(s) 104, 186, 580, 1844, 2697
BseMII CTCAG 7 cut(s) 153, 272, 1626, 1763, 2549, 3021, 3165
BseNI ACTGG 9 cut(s) 301, 346, 761, 1158, 1426, 1923, 2522, 2717, 3362
BseRI GAGGAG 2 cut(s) 586, 3191
BseSI GKGCMC 2 cut(s) 1251, 3289
BseXI GCAGC 8 cut(s) 25, 43, 85, 1352, 2087, 2714, 3140, 3344
BseYI CCCAGC 1 cut(s) 109
BsgI GTGCAG 5 cut(s) 32, 50, 288, 2094, 3351
Bsh1285I CGRYCG 1 cut(s) 1859
BshFI GGCC 4 cut(s) 300, 579, 1426, 3117
BshNI GGYRCC 1 cut(s) 1999
BsiEI CGRYCG 1 cut(s) 1859
BsiHKAI GWGCWC 5 cut(s) 401, 1251, 1334, 2649, 3289
BsiSI CCGG 3 cut(s) 77, 2350, 3073
BslFI GGGAC 4 cut(s) 249, 1053, 1695, 2275
BslI CCNNNNNNNGG 7 cut(s) 83, 942, 1047, 1996, 2837, 3124, 3169
BsmAI GTCTC 7 cut(s) 234, 404, 536, 737, 926, 1541, 2039
BsmBI CGTCTC 1 cut(s) 234
BsmFI GGGAC 4 cut(s) 249, 1053, 1695, 2275
BsmI GAATGC 2 cut(s) 1212, 2078
BsnI GGCC 4 cut(s) 300, 579, 1426, 3117
Bso31I GGTCTC 1 cut(s) 926
Bsp1286I GDGCHC 5 cut(s) 401, 1251, 1334, 2649, 3289
Bsp1407I TGTACA 1 cut(s) 2362
Bsp19I CCATGG 3 cut(s) 784, 1990, 3093
BspACI CCGC 7 cut(s) 71, 385, 423, 1228, 1613, 2477, 3199
BspANI GGCC 4 cut(s) 300, 579, 1426, 3117
BspCNI CTCAG 7 cut(s) 154, 271, 1627, 1762, 2548, 3022, 3166
BspHI TCATGA 1 cut(s) 195
BspLI GGNNCC 7 cut(s) 254, 659, 1068, 1069, 1156, 2001, 3100
BspMI ACCTGC 1 cut(s) 3317
BspOI GCTAGC 1 cut(s) 1373
BspPI GGATC 9 cut(s) 51, 1623, 1685, 1877, 1892, 2264, 2528, 2947, 3154
BspT107I GGYRCC 1 cut(s) 1999
BspTI CTTAAG 1 cut(s) 59
BspTNI GGTCTC 1 cut(s) 926
BsrDI GCAATG 5 cut(s) 104, 186, 580, 1844, 2697
BsrGI TGTACA 1 cut(s) 2362
BsrI ACTGG 9 cut(s) 301, 346, 761, 1158, 1426, 1923, 2522, 2717, 3362
BssECI CCNNGG 7 cut(s) 661, 784, 1041, 1144, 1990, 2567, 3093
BssNAI GTATAC 1 cut(s) 1583
BssNI GRCGYC 1 cut(s) 123
BssSI CACGAG 1 cut(s) 2049
BssT1I CCWWGG 6 cut(s) 661, 784, 1144, 1990, 2567, 3093
Bst1107I GTATAC 1 cut(s) 1583
Bst2BI CACGAG 1 cut(s) 2049
Bst2UI CCWGG 5 cut(s) 432, 777, 1737, 2358, 3372
Bst6I CTCTTC 3 cut(s) 348, 604, 2037
BstACI GRCGYC 1 cut(s) 123
BstAFI CTTAAG 1 cut(s) 59
BstAUI TGTACA 1 cut(s) 2362
BstBAI YACGTR 3 cut(s) 133, 1414, 2397
BstC8I GCNNGC 7 cut(s) 11, 102, 116, 948, 1371, 1375, 3344
BstDSI CCRYGG 4 cut(s) 784, 1041, 1990, 3093
BstEII GGTNACC 1 cut(s) 779
BstENI CCTNNNNNAGG 1 cut(s) 3167
BstF5I GGATG 7 cut(s) 554, 772, 1051, 2485, 2721, 2730, 2965
BstH2I RGCGCY 1 cut(s) 904
BstHHI GCGC 1 cut(s) 903
BstMAI GTCTC 7 cut(s) 234, 404, 536, 737, 926, 1541, 2039
BstMCI CGRYCG 1 cut(s) 1859
BstMWI GCNNNNNNNGC 8 cut(s) 37, 73, 106, 115, 1619, 2825, 3098, 3332
BstNI CCWGG 5 cut(s) 432, 777, 1737, 2358, 3372
BstNSI RCATGY 1 cut(s) 950
BstPAI GACNNNNGTC 1 cut(s) 2326
BstPI GGTNACC 1 cut(s) 779
BstSCI CCNGG 5 cut(s) 430, 775, 1735, 2356, 3370
BstSFI CTRYAG 1 cut(s) 1097
BstSLI GKGCMC 2 cut(s) 1251, 3289
BstV1I GCAGC 8 cut(s) 25, 43, 85, 1352, 2087, 2714, 3140, 3344
BstV2I GAAGAC 5 cut(s) 835, 2672, 2882, 2982, 3360
BstX2I RGATCY 7 cut(s) 1397, 1615, 1882, 1897, 2520, 2981, 3159
BstYI RGATCY 7 cut(s) 1397, 1615, 1882, 1897, 2520, 2981, 3159
BstZ17I GTATAC 1 cut(s) 1583
Bsu36I CCTNAGG 1 cut(s) 3030
BsuRI GGCC 4 cut(s) 300, 579, 1426, 3117
BtgI CCRYGG 4 cut(s) 784, 1041, 1990, 3093
BtsCI GGATG 7 cut(s) 554, 772, 1051, 2485, 2721, 2730, 2965
BtsIMutI CAGTG 7 cut(s) 573, 1258, 1419, 1914, 1916, 2710, 3399
BveI ACCTGC 1 cut(s) 3317
Cac8I GCNNGC 7 cut(s) 11, 102, 116, 948, 1371, 1375, 3344
CaiI CAGNNNCTG 1 cut(s) 1756
CciI TCATGA 1 cut(s) 195
CfoI GCGC 1 cut(s) 903
Cfr13I GGNCC 8 cut(s) 139, 252, 299, 788, 1067, 1424, 2462, 3115
CseI GACGC 3 cut(s) 131, 1417, 3233
CsiI ACCWGGT 1 cut(s) 3370
Csp6I GTAC 5 cut(s) 1185, 1415, 2347, 2363, 2437
CspCI CAANNNNNGTGG 4 cut(s) 1010, 1045, 3286, 3321
CviQI GTAC 5 cut(s) 1185, 1415, 2347, 2363, 2437
DraI TTTAAA 1 cut(s) 2554
DraIII CACNNNGTG 1 cut(s) 1246
EaeI YGGCCR 1 cut(s) 577
Eam1104I CTCTTC 3 cut(s) 348, 604, 2037
EarI CTCTTC 3 cut(s) 348, 604, 2037
Ecl136II GAGCTC 3 cut(s) 399, 1332, 2647
Eco130I CCWWGG 6 cut(s) 661, 784, 1144, 1990, 2567, 3093
Eco24I GRGCYC 3 cut(s) 401, 1334, 2649
Eco31I GGTCTC 1 cut(s) 926
Eco47I GGWCC 5 cut(s) 139, 252, 788, 1067, 2462
Eco53kI GAGCTC 3 cut(s) 399, 1332, 2647
Eco57I CTGAAG 4 cut(s) 372, 462, 513, 2798
Eco81I CCTNAGG 1 cut(s) 3030
Eco91I GGTNACC 1 cut(s) 779
EcoICRI GAGCTC 3 cut(s) 399, 1332, 2647
EcoNI CCTNNNNNAGG 1 cut(s) 3167
EcoO109I RGGNCCY 1 cut(s) 1067
EcoO65I GGTNACC 1 cut(s) 779
EcoRI GAATTC 2 cut(s) 1529, 1912
EcoRII CCWGG 5 cut(s) 430, 775, 1735, 2356, 3370
EcoT14I CCWWGG 6 cut(s) 661, 784, 1144, 1990, 2567, 3093
EcoT22I ATGCAT 1 cut(s) 1389
EcoT38I GRGCYC 3 cut(s) 401, 1334, 2649
ErhI CCWWGG 6 cut(s) 661, 784, 1144, 1990, 2567, 3093
Esp3I CGTCTC 1 cut(s) 234
FalI AAGNNNNNCTT 8 cut(s) 1055, 1087, 1200, 1232, 1641, 1673, 2551, 2583
FaqI GGGAC 4 cut(s) 249, 1053, 1695, 2275
FbaI TGATCA 2 cut(s) 198, 325
FblI GTMKAC 2 cut(s) 342, 1582
FokI GGATG 7 cut(s) 541, 759, 1058, 2492, 2728, 2737, 2972
FriOI GRGCYC 3 cut(s) 401, 1334, 2649
GlaI GCGC 1 cut(s) 902
GsaI CCCAGC 1 cut(s) 113
GsuI CTGGAG 4 cut(s) 876, 925, 1940, 3379
HaeII RGCGCY 1 cut(s) 904
HaeIII GGCC 4 cut(s) 300, 579, 1426, 3117
HapII CCGG 3 cut(s) 77, 2350, 3073
HgaI GACGC 3 cut(s) 131, 1417, 3233
HhaI GCGC 1 cut(s) 903
Hin1I GRCGYC 1 cut(s) 123
Hin6I GCGC 1 cut(s) 901
HinP1I GCGC 1 cut(s) 901
HincII GTYRAC 1 cut(s) 3022
HindII GTYRAC 1 cut(s) 3022
HindIII AAGCTT 4 cut(s) 495, 1575, 2034, 2683
HpaII CCGG 3 cut(s) 77, 2350, 3073
Hpy99I CGWCG 3 cut(s) 86, 987, 2114
HpyCH4IV ACGT 9 cut(s) 132, 1413, 2055, 2071, 2396, 2467, 2879, 3024, 3243
HpyF10VI GCNNNNNNNGC 8 cut(s) 37, 73, 106, 115, 1619, 2825, 3098, 3332
HpySE526I ACGT 9 cut(s) 132, 1413, 2055, 2071, 2396, 2467, 2879, 3024, 3243
Hsp92I GRCGYC 1 cut(s) 123
HspAI GCGC 1 cut(s) 901
KflI GGGWCCC 1 cut(s) 1067
Ksp22I TGATCA 2 cut(s) 198, 325
LmnI GCTCC 2 cut(s) 628, 3098
Lsp1109I GCAGC 8 cut(s) 25, 43, 85, 1352, 2087, 2714, 3140, 3344
LweI GCATC 4 cut(s) 96, 1327, 1374, 2815
MabI ACCWGGT 1 cut(s) 3370
MaeII ACGT 9 cut(s) 132, 1413, 2055, 2071, 2396, 2467, 2879, 3024, 3243
MfeI CAATTG 1 cut(s) 2795
MflI RGATCY 7 cut(s) 1397, 1615, 1882, 1897, 2520, 2981, 3159
MhlI GDGCHC 5 cut(s) 401, 1251, 1334, 2649, 3289
MlsI TGGCCA 1 cut(s) 579
MluNI TGGCCA 1 cut(s) 579
MlyI GAGTC 7 cut(s) 17, 271, 357, 538, 1553, 2041, 3399
MmeI TCCRAC 4 cut(s) 1801, 2297, 2404, 2523
Mox20I TGGCCA 1 cut(s) 579
Mph1103I ATGCAT 1 cut(s) 1389
MroXI GAANNNNTTC 1 cut(s) 1212
MscI TGGCCA 1 cut(s) 579
MseI TTAA 7 cut(s) 60, 845, 869, 1199, 2217, 2553, 2844
MslI CAYNNNNRTG 3 cut(s) 777, 1590, 2370
Msp20I TGGCCA 1 cut(s) 579
MspA1I CMGCKG 3 cut(s) 385, 2267, 2539
MspCI CTTAAG 1 cut(s) 59
MspI CCGG 3 cut(s) 77, 2350, 3073
MspR9I CCNGG 5 cut(s) 432, 777, 1737, 2358, 3372
MssI GTTTAAAC 1 cut(s) 2554
MunI CAATTG 1 cut(s) 2795
Mva1269I GAATGC 2 cut(s) 1212, 2078
MvaI CCWGG 5 cut(s) 432, 777, 1737, 2358, 3372
MwoI GCNNNNNNNGC 8 cut(s) 37, 73, 106, 115, 1619, 2825, 3098, 3332
NcoI CCATGG 3 cut(s) 784, 1990, 3093
NheI GCTAGC 1 cut(s) 1369
NlaIV GGNNCC 7 cut(s) 254, 659, 1068, 1069, 1156, 2001, 3100
NmuCI GTSAC 6 cut(s) 53, 748, 779, 1238, 1497, 2051
NsiI ATGCAT 1 cut(s) 1389
NspI RCATGY 1 cut(s) 950
OliI CACNNNNGTG 1 cut(s) 2370
PaeI GCATGC 1 cut(s) 950
PagI TCATGA 1 cut(s) 195
PaqCI CACCTGC 1 cut(s) 3317
PcsI WCGNNNNNNNCGW 1 cut(s) 129
PctI GAATGC 2 cut(s) 1212, 2078
PdmI GAANNNNTTC 1 cut(s) 1212
PflMI CCANNNNNTGG 1 cut(s) 942
PleI GAGTC 7 cut(s) 17, 270, 357, 538, 1552, 2041, 3398
PmeI GTTTAAAC 1 cut(s) 2554
PpsI GAGTC 7 cut(s) 17, 270, 357, 538, 1552, 2041, 3398
Ppu21I YACGTR 3 cut(s) 133, 1414, 2397
PpuMI RGGWCCY 1 cut(s) 1067
PshAI GACNNNNGTC 1 cut(s) 2326
PsiI TTATAA 2 cut(s) 726, 1365
Psp124BI GAGCTC 3 cut(s) 401, 1334, 2649
Psp5II RGGWCCY 1 cut(s) 1067
Psp6I CCWGG 5 cut(s) 430, 775, 1735, 2356, 3370
PspEI GGTNACC 1 cut(s) 779
PspFI CCCAGC 1 cut(s) 109
PspGI CCWGG 5 cut(s) 430, 775, 1735, 2356, 3370
PspN4I GGNNCC 7 cut(s) 254, 659, 1068, 1069, 1156, 2001, 3100
PspPI GGNCC 8 cut(s) 139, 252, 299, 788, 1067, 1424, 2462, 3115
PspPPI RGGWCCY 1 cut(s) 1067
PstNI CAGNNNCTG 1 cut(s) 1756
PsuI RGATCY 7 cut(s) 1397, 1615, 1882, 1897, 2520, 2981, 3159
PvuII CAGCTG 2 cut(s) 2267, 2539
RsaI GTAC 5 cut(s) 1186, 1416, 2348, 2364, 2438
RsaNI GTAC 5 cut(s) 1185, 1415, 2347, 2363, 2437
RseI CAYNNNNRTG 3 cut(s) 777, 1590, 2370
SacI GAGCTC 3 cut(s) 401, 1334, 2649
SaqAI TTAA 7 cut(s) 60, 845, 869, 1199, 2217, 2553, 2844
Sau96I GGNCC 8 cut(s) 139, 252, 299, 788, 1067, 1424, 2462, 3115
SchI GAGTC 7 cut(s) 17, 271, 357, 538, 1553, 2041, 3399
ScrFI CCNGG 5 cut(s) 432, 777, 1737, 2358, 3372
SduI GDGCHC 5 cut(s) 401, 1251, 1334, 2649, 3289
SexAI ACCWGGT 1 cut(s) 3370
SfaNI GCATC 4 cut(s) 96, 1327, 1374, 2815
SfcI CTRYAG 1 cut(s) 1097
SinI GGWCC 5 cut(s) 139, 252, 788, 1067, 2462
SmiMI CAYNNNNRTG 3 cut(s) 777, 1590, 2370
SmlI CTYRAG 6 cut(s) 59, 333, 402, 818, 1706, 2524
SmoI CTYRAG 6 cut(s) 59, 333, 402, 818, 1706, 2524
SpeI ACTAGT 1 cut(s) 536
SphI GCATGC 1 cut(s) 950
SsiI CCGC 7 cut(s) 71, 385, 423, 1228, 1613, 2477, 3199
SspI AATATT 1 cut(s) 843
SstI GAGCTC 3 cut(s) 401, 1334, 2649
StyD4I CCNGG 5 cut(s) 430, 775, 1735, 2356, 3370
StyI CCWWGG 6 cut(s) 661, 784, 1144, 1990, 2567, 3093
TaiI ACGT 9 cut(s) 135, 1416, 2058, 2074, 2399, 2470, 2882, 3027, 3246
TaqII GACCGA 1 cut(s) 996
TatI WGTACW 1 cut(s) 2362
TauI GCSGC 3 cut(s) 388, 1231, 3202
Tru1I TTAA 7 cut(s) 60, 845, 869, 1199, 2217, 2553, 2844
Tru9I TTAA 7 cut(s) 60, 845, 869, 1199, 2217, 2553, 2844
TscAI CASTG 7 cut(s) 573, 1258, 1426, 1914, 1923, 2717, 3406
TseFI GTSAC 6 cut(s) 53, 748, 779, 1238, 1497, 2051
TseI GCWGC 8 cut(s) 13, 31, 73, 1340, 2075, 2702, 3128, 3332
Tsp45I GTSAC 6 cut(s) 53, 748, 779, 1238, 1497, 2051
TspGWI ACGGA 3 cut(s) 16, 1030, 2461
TspRI CASTG 7 cut(s) 573, 1258, 1426, 1914, 1923, 2717, 3406
Van91I CCANNNNNTGG 1 cut(s) 942
Vha464I CTTAAG 1 cut(s) 59
VneI GTGCAC 2 cut(s) 1247, 3285
VpaK11BI GGWCC 5 cut(s) 139, 252, 788, 1067, 2462
XagI CCTNNNNNAGG 1 cut(s) 3167
XbaI TCTAGA 2 cut(s) 239, 2635
XceI RCATGY 1 cut(s) 950
XcmI CCANNNNNNNNNTGG 2 cut(s) 2565, 2597
XmiI GTMKAC 2 cut(s) 342, 1582
XmnI GAANNNNTTC 1 cut(s) 1212
Zsp2I ATGCAT 1 cut(s) 1389
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.