Rh2AG064800
ERF Family

Probably involved in the RNA silencing pathway and required for the generation of small interfering RNAs (siRNAs)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0000505
Physical Location & Seq
Forward (+)
5350711 .. 5361680
10970 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG064800.1

Sequence Viewer

Length: 4239 bp
ATGATGAAGCAGAGTTGGGATGAGCAAGAAGAGGTGCCGCGTCTACCTCAGAAAGTGGAGGTATTGCTCCGGCAAATCTGTGAGGACAAAAAGCAACCTTGGCCGGACGCTGACGCCATAAGGCAGCTCGCGACTCAGAGCGAGGAGGCGGCAATGGAGACCCTGAAGAAAATCTCCACCAGCAAAAGCATCAGAACCACCCTCGGCCGTTACATCAAGTGGATGATCGGGCAATGTAGCTCTGCTTCTCCGTCGCCGTCCAAAACGGTGCGTTCCGCTCACGTTCAAAGCCCTGGTGCTACTTCACTCACTCCTCAGAGACTTCACTTCAGCCCTCCAGGTGAGGCAGTTAGTGACAGGTCACCTGTTGTTGGGCAATTACCTTATTGCTCTGGTTCTCCATCCAAAGCCGCGGGGACTTGTTCTTTTCAAAGATTGTCTGCTCATGCACAAGTTGATGAGAGGTCTCGAGGCGAGGCATTTAATCAGCTGTCACCTGTTATTGGTCAATCACCTTATTCCTCTGCTTCTCCTTTGAAAGCCGCAGACACTTCTTCTTATCAAAGACTCTCTGCTCCTGTACAACTGGATGAGAGATTTCAAGGTGATGCATTTAGTCATCGGTCACCTGTTGGACAACCACCTCATTCCTCTGCTTCTCCATCAAAAGCTTCAGGCACTACTTCTTATCAAAGATTGTCTCCTCTTGATGATACGTTTCAAGGCACTTCTTATCAAAGACTGTCTGCTCCTGTCCAACATGATGAAAGGTTTCAAGGTGATGCATTTAGTCATCGGTCATCTGTTGGGCAACCACCTTATTCCTCTGCTTCTCCATCAAAAGCTGCAGGCACTACTTCTTATCAAAGATTGTCTCCTCCTGTACAACTTGATGATACGTTTCAAGGTGAGGCATTTACTGTTCTAGAGCAACCAGGAAACATCAATAATGGACAATCACCTTATTATTCCTCTGGTTCTCCATTGAAAGTGGCACGCACTTGTTCTTGTCAAAGCCCAGCTGCGTTGGTTGCTCCTATACAACTTGATAAGAAGTTTCAAGGTGACCCATTTACTGACAGGTCACTTGTTCGACAATTACCTTGTTCCTCTGGTTCTCCATTAAAAGCCACAGGTTCTTATGAAAGATTGTCTACTCCTGCACAAGCTGATGAGCGGTTTCATGGTGAGGCATTTAGTGCCCGGTCAGCTGTTGGACAATTGCCTTGTTCCTCTGTTTCTCCATTAAAATCCGCAGTCACTTCTTCTTATCAAAGATTGTCTACTCCTGTACAGCTTGATGAGAGGTTTCAAGTTGAGGCATTTAATGACCGGTCACCTGTTATGGGACAATCACCTTACTCTGGTTCTCCATTGAAAGCTGCAAGCACTTCTTATCAAAGACCGACTGCTCCTCTACAACTTGATGAGAGGGTTCAAGGTGGGGTATTTGCTGATCAGACACCTGTTCAAGGACAATCACCATATGCCTCTTGTTCTCCATCAAAAGCCTTCTGCATTTCTCCTTATCAGAGAATGTCTGCTGCTGTTGTTCCTGGACAACTTCATGAAAGGTTTCAAGGTGAGGGTATCACTAGGTCCTCAGCTATAGACAAACCTGGAGATCATGGAGAAGCACACTTAGTAGCTCTGGGTGAACTAGAATTCCGTAAACAATTTCTGATTTTAAGTTATGCTGGAGGAACGAAGTTGAGAGATCTTATAGCGGCAGAGGACATTAGGGATTGGAAATATTTGCCAATGGTAATGTTTGAGAGCAAAATTTGGGAGTATCTAGGTCGAAAATGCATCAGCCTCGAAGACCGTCGATTGAATTTTGATTGGGATTCTGGACGCACACACGTCTATCATTGTTACGTCAACGTGGATGGGAGTTACATATTTAAGGGCCCATATTTATATAAAATAAGAAGAACGCTCCTACAGAAAAAATTAGGAGATGACAATGTTTTAATGGTGAAGTTTGCCAAAGAAGAGGTGCAGAGAGGTCAAAGTATTGCCTGCCATCATAATTATTCCATGTATATGAAGGTTGCAAGAGAAGGGATTCTTGTTGGTTTGCGGCGATATCGTTTCTTTGTGTTCAAAGATGGTGGTAATGAGGAGAAGAAAAAAAACCCGACTTCATCACCTGTGAAGTGTTACTTTGTTCGAGTGACCTCTGATGCAGCCATAGACAAAGGTGTCAGTTATATATTATCTAACAGAACGATGTATGAAGCCAGATGTCTTTTCATGCATGCACATACTCTGGCTAGGATCGATAACTACATGGTTAGGTTCTCACTCATTTTGTCCAAGACAACGAGCTTAAAAGTAGATTGGTCAGTTGTCAAGGTTGAAATCATCGGTGAAGAGTACTGTCTCGATGAATCTGGTAATCGTATCCATAGAGATGAAAAACCCCTTATACATACAGATGGAACTGGATTCATATCCGAGGATTTGGCTTTAATTTGTCCAAAGATAGAAGCCAAAGGACAATGTATAAGTGATGAACACATGAAGGGACTTCCTGATCCTGATGAACTTGAGGATGATGATATGGGAAAGAAAAGGCCAGTTCTTAGAACAGAAGAACCGCCTTTGCTCACACAGTGCCGACTTTTTCACAATGGCAGTGCTTCTAAAGGAACGTTTCTTGTCAATAAAAAGCTCCATCCGAACACAATCCAGATTCGACCATCCATGCTCAAAGTTAAGACAGATCCAACGATTTCCAATAATCAAACAATAAACTCATTGGAAATAGTTGGCACAAGCACTCCTCCTAGGAATACGCACTTATCGAGGATTTTAATTGCACTTCTAATGCATGGAGGGGTACCGGAAGAATTTTTCATGCAGATACTTAACCAAGCTTTGGAAGATGCTCATGGTGTTTTCTGCAAGACACGTGCAGCACTCAAAGTTGCTGAGAAGTATGGTGAGATTGATGACAATTTCTTGGGGAAATTAATGATCTCATTTGGAATACCTCTAGAAGAATCATACTTGCGGTATCGTCTCTCAGTCCTAATGAAAGAGGAAAACAACAGTCTTAGAAAAGGGAAACTTTATATTCCTGATTCCTATAATTTGATGGGGACAGCTGATCCAACAGGGACTTTAAAAAAAGATGAAGTCTGTGTTATCCTGAAGGATGGACAGCTGTCGGGGAAGGTACTAGTCTACAGATATCCGGGTTTACACTTTGGGGATATTCATGTTCTAAAGGCGACCTATGTGAAGGAGTTAGAATCTTTTGTTGGAAATGCCAAATACGGTATATTCTTCTCTTGCAAAGGTCCACGTTCCATTGCTGATGAAATGGGTGGTGGAGATTTTGATGGTGATCTATACTGGGTCTCAAGAAACCCTCAGCTATTGGAGTGGTACAAACCAAGTGAACCTTGGATTGAAGCTTCAATATCAATCCAAAAAGTTCCCAGTAGGAGTCCATCTGACTATTCGCCTGAAGATCTAGAAGATCTTCTGTTTAGATCATTCTTGACAACGAGGTTTGAACCAAGTTTCGCTATGAGTGAGGCAGCTGATAATTGGCTAGCCTGGATGGATCGTTTCTTGACGTTGGGAGAGAGTGACATTGACGAAAAGAATAATATGAAGAAGAAAATACTGCAACTAATTAACTTATACTACACAGCTCTAGATGCACCGAAGAAAGGTGTAAAGGTTGTAGTCCCAAAAGAATTGAAGGCCCCATGTTTCCCCCATTACATGGGAAAGAACAATTCTTACCCTTCAACTTCCGTTCTGGGATTAATTTATAACAGGGTGGATGAGCATCAAACAGAAGACCGTTCTCTCAAAGAAGTCGAGAAACTTCCTGCTTTTGATGTTAATGAAGTCCCTGATGGCTGCTTGGACAAATGGGAGGAACTACACCGACAGTACAGGAGTGAGATGGCCTCTGCTATGGACGATGCTGATAAACAGAGCAAAAATCAGTCTGCCGATGTGGTCATAAGGAAATATAAAAAGATTCTTTACGAGGCTAATGACTTTGAGGAGAGTAGAAGGCCAATCGATGAGATATATAACGAGGCACAAGCTATATATCATGTGTGTTATGACCATGCGAGGAGGATAGGAAGTGTGAAGGCGTGTTCCTTTGCATGGAGAGTTGCAGGTAAAGCTCTGTGCCAGCTTCACGCCAAGAACACGAATGAAAGGTGTTGTACGATAGTATCCTCTGTTTTGAAGGAGATTTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RdRP

Protein Analysis

1412

Amino Acids

157.96

Weight (kDa)

8.03

Isoelectric Point (pI)

48.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RdRP_helical PF26252 547 - 611 4.2e-07 RNA dependent RNA polymerase, helical domain
RdRP PF05183 633 - 1267 3.7e-99 RNA dependent RNA polymerase
RdRP_head PF26253 1296 - 1396 8.5e-14 RdRP, head domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000505)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G19910 AT2G19910 AT2G19920 AT2G19920 AT2G19920 AT2G19920 AT2G19930 AT2G19930 AT2G19930
fragaria_vesca FvH4_1g05980 FvH4_1g05990 FvH4_1g05990 FvH4_1g09718
malus_domestica MD02G1064400.v1.1 MD15G1195300.v1.1 MD15G1195400.v1.1
prunus_persica Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1
pyrus_communis pycom02g05070 pycom15g17460
rosa_chinensis RchiOBHm_Chr2g0091671 RchiOBHm_Chr2g0091681 RchiOBHm_Chr2g0091691 RchiOBHm_Chr2g0091701 RchiOBHm_Chr2g0091711 RchiOBHm_Chr2g0091721 RchiOBHm_Chr2g0091731 RchiOBHm_Chr5g0014401
rosa_laevigata RLG00000013772 RLG00000016242 RLG00000016244 RLG00000016245 RLG00000016246 RLG00000032105
rosa_multiflora Rmu_co8374041.1_g000001 Rmu_co8478121.1_g000001 Rmu_sc0000897.1_g000005 Rmu_sc0000897.1_g000009
rosa_roxburghii Rroxscaffold_1G00062180 Rroxscaffold_1G00062190 Rroxscaffold_1G00068800 Rroxscaffold_2G00149790 Rroxscaffold_2G00149800 Rroxscaffold_2G00149810
rosa_rugosa Rorug02G0019600 Rorug02G0019700 Rorug02G0019800 Rorug02G0019900 Rorug02G0019900 Rorug02G0020000 Rorug02G0020000 Rorug02G0020100 Rorug02G0020100 Rorug02G0020200 Rorug05G0017800
rosa_samantha Rh2AG064700 Rh2AG064800 Rh2AG064900 Rh2BG063800 Rh2BG063900 Rh2BG064000 Rh2BG064100 Rh2CG065500 Rh2CG065600 Rh2CG065700 Rh2DG063900 Rh2DG064000 Rh2DG064100 Rh2DG064200 Rh2DG381700 Rh5AG056700 Rh5AG056800 Rh5AG056900 Rh5AG112600 Rh5BG109300 Rh5CG063800 Rh5CG121100 Rh5DG053100 Rh5DG108300 Rh6CG100100
rosa_wichuraiana Rw2G005560 Rw2G005580 Rw2G005590 Rw5G005370 Rw5G009770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3792
AasI GACNNNNNNGTC 1 cut(s) 2204
Acc36I ACCTGC 1 cut(s) 4142
Acc65I GGTACC 1 cut(s) 2845
AccB1I GGYRCC 2 cut(s) 34, 2845
AccB7I CCANNNNNTGG 2 cut(s) 2884, 3742
AccBSI CCGCTC 2 cut(s) 278, 1177
AccI GTMKAC 4 cut(s) 43, 1154, 1283, 3192
AccII CGCG 3 cut(s) 40, 131, 413
AclI AACGTT 1 cut(s) 2657
AclWI GGATC 5 cut(s) 2288, 2534, 2723, 3110, 3585
AcoI YGGCCR 2 cut(s) 101, 205
AcsI RAATTY 4 cut(s) 1664, 1782, 1834, 2855
AcuI CTGAAG 5 cut(s) 185, 313, 657, 3179, 3498
AcvI CACGTG 1 cut(s) 2918
AcyI GRCGYC 1 cut(s) 114
AdeI CACNNNGTG 1 cut(s) 2619
AfaI GTAC 9 cut(s) 582, 885, 1293, 2381, 2847, 3186, 3398, 3917, 4204
AfiI CCNNNNNNNGG 9 cut(s) 371, 503, 1346, 1364, 1472, 2884, 3686, 3742, 4140
AflIII ACRYGT 2 cut(s) 1861, 2915
AgeI ACCGGT 1 cut(s) 1332
AhlI ACTAGT 1 cut(s) 3187
AjiI CACGTC 1 cut(s) 1864
AjnI CCWGG 6 cut(s) 292, 337, 934, 1555, 1618, 3569
AjuI GAANNNNNNNTTGG 2 cut(s) 3252, 3284
AloI GAACNNNNNNTCC 2 cut(s) 1650, 1682
Alw26I GTCTC 8 cut(s) 152, 313, 471, 705, 879, 2390, 3032, 3373
AlwI GGATC 5 cut(s) 2288, 2534, 2723, 3110, 3585
Ama87I CYCGRG 1 cut(s) 468
AoxI GGCC 7 cut(s) 101, 205, 1909, 2579, 3720, 3930, 4043
ApaI GGGCCC 1 cut(s) 1913
ApeKI GCWGC 9 cut(s) 124, 845, 1022, 1382, 1544, 2189, 2921, 3551, 3882
ApoI RAATTY 4 cut(s) 1664, 1782, 1834, 2855
AseI ATTAAT 2 cut(s) 2978, 3785
AsiGI ACCGGT 1 cut(s) 1332
Asp700I GAANNNNTTC 4 cut(s) 771, 1575, 2067, 3492
Asp718I GGTACC 1 cut(s) 2845
AspA2I CCTAGG 1 cut(s) 2792
AspS9I GGNCC 5 cut(s) 1599, 1909, 1910, 3308, 3721
AsuC2I CCSGG 2 cut(s) 1204, 3204
AsuNHI GCTAGC 1 cut(s) 3565
AvaI CYCGRG 1 cut(s) 468
AvaII GGWCC 2 cut(s) 1599, 3308
AvrII CCTAGG 1 cut(s) 2792
BaeGI GKGCMC 2 cut(s) 1204, 1913
BaeI ACNNNNGTAYC 2 cut(s) 4194, 4227
BanI GGYRCC 2 cut(s) 34, 2845
BanII GRGCYC 1 cut(s) 1913
BarI GAAGNNNNNNTAC 2 cut(s) 229, 261
BbrPI CACGTG 1 cut(s) 2918
BbsI GAAGAC 2 cut(s) 1827, 3825
BbvCI CCTCAGC 2 cut(s) 1603, 3381
BbvI GCAGC 9 cut(s) 136, 832, 1009, 1369, 1531, 2201, 2933, 3563, 3869
BceAI ACGGC 2 cut(s) 192, 241
BcgI CGANNNNNNTGC 2 cut(s) 1798, 1832
BciT130I CCWGG 6 cut(s) 294, 339, 936, 1557, 1620, 3571
BciVI GTATCC 2 cut(s) 2417, 4222
BclI TGATCA 1 cut(s) 1456
BcnI CCSGG 2 cut(s) 1204, 3204
BcoDI GTCTC 8 cut(s) 152, 313, 471, 705, 879, 2390, 3032, 3373
BcuI ACTAGT 1 cut(s) 3187
BfmI CTRYAG 4 cut(s) 846, 1608, 1943, 3193
BfuAI ACCTGC 1 cut(s) 4142
BfuI GTATCC 2 cut(s) 2417, 4222
BglII AGATCT 3 cut(s) 1717, 3481, 3490
BlnI CCTAGG 1 cut(s) 2792
BmcAI AGTACT 1 cut(s) 2381
Bme1390I CCNGG 8 cut(s) 294, 339, 936, 1204, 1557, 1620, 3204, 3571
Bme18I GGWCC 2 cut(s) 1599, 3308
BmeT110I CYCGRG 1 cut(s) 468
BmgBI CACGTC 1 cut(s) 1864
BmgT120I GGNCC 5 cut(s) 1599, 1909, 1910, 3308, 3721
BmiI GGNNCC 4 cut(s) 36, 1911, 2847, 3723
BmrFI CCNGG 8 cut(s) 294, 339, 936, 1204, 1557, 1620, 3204, 3571
BmrI ACTGGG 2 cut(s) 3373, 3444
BmsI GCATC 9 cut(s) 198, 598, 772, 1818, 2176, 2881, 3664, 3817, 3937
BmtI GCTAGC 1 cut(s) 3569
BmuI ACTGGG 2 cut(s) 3373, 3444
BoxI GACNNNNGTC 1 cut(s) 3172
BpiI GAAGAC 2 cut(s) 1827, 3825
BplI GAGNNNNNCTC 2 cut(s) 3917, 3949
BpmI CTGGAG 3 cut(s) 321, 1641, 1719
Bpu10I CCTNAGC 2 cut(s) 1603, 3381
BpuEI CTTGAG 2 cut(s) 2573, 3355
BpuMI CCSGG 2 cut(s) 1204, 3204
Bsa29I ATCGAT 2 cut(s) 2283, 4050
BsaAI YACGTR 1 cut(s) 2918
BsaBI GATNNNNATC 3 cut(s) 2455, 3354, 3807
BsaHI GRCGYC 1 cut(s) 114
BsaI GGTCTC 3 cut(s) 152, 471, 3373
BsaJI CCNNGG 7 cut(s) 98, 202, 292, 411, 2460, 2792, 3413
BsaWI WCCGGW 2 cut(s) 1332, 2848
BsaXI ACNNNNNCTCC 3 cut(s) 2770, 2800, 4220
Bsc4I CCNNNNNNNGG 9 cut(s) 371, 503, 1346, 1364, 1472, 2884, 3686, 3742, 4140
Bse118I RCCGGY 1 cut(s) 1332
Bse1I ACTGG 5 cut(s) 591, 2452, 2582, 3368, 3450
Bse3DI GCAATG 3 cut(s) 159, 239, 3318
Bse8I GATNNNNATC 3 cut(s) 2455, 3354, 3807
BseBI CCWGG 6 cut(s) 294, 339, 936, 1557, 1620, 3571
BseCI ATCGAT 2 cut(s) 2283, 4050
BseDI CCNNGG 7 cut(s) 98, 202, 292, 411, 2460, 2792, 3413
BseJI GATNNNNATC 3 cut(s) 2455, 3354, 3807
BseLI CCNNNNNNNGG 9 cut(s) 371, 503, 1346, 1364, 1472, 2884, 3686, 3742, 4140
BseMI GCAATG 3 cut(s) 159, 239, 3318
BseMII CTCAG 7 cut(s) 62, 149, 329, 1617, 2928, 3045, 3395
BseNI ACTGG 5 cut(s) 591, 2452, 2582, 3368, 3450
BseRI GAGGAG 9 cut(s) 158, 303, 693, 867, 1404, 2138, 2778, 4046, 4120
BseSI GKGCMC 2 cut(s) 1204, 1913
BseX3I CGGCCG 1 cut(s) 205
BseXI GCAGC 9 cut(s) 136, 832, 1009, 1369, 1531, 2201, 2933, 3563, 3869
BseYI CCCAGC 1 cut(s) 1018
BsgI GTGCAG 3 cut(s) 1146, 2021, 2940
Bsh1236I CGCG 3 cut(s) 40, 131, 413
Bsh1285I CGRYCG 1 cut(s) 208
BshFI GGCC 7 cut(s) 103, 207, 1911, 2581, 3722, 3932, 4045
BshNI GGYRCC 2 cut(s) 34, 2845
BshTI ACCGGT 1 cut(s) 1332
BshVI ATCGAT 2 cut(s) 2283, 4050
BsiEI CGRYCG 1 cut(s) 208
BsiHKCI CYCGRG 1 cut(s) 468
BsiSI CCGG 6 cut(s) 70, 104, 1204, 1333, 2849, 3203
BslFI GGGAC 7 cut(s) 430, 1362, 2544, 3121, 3139, 3689, 3857
BslI CCNNNNNNNGG 9 cut(s) 371, 503, 1346, 1364, 1472, 2884, 3686, 3742, 4140
BsmAI GTCTC 8 cut(s) 152, 313, 471, 705, 879, 2390, 3032, 3373
BsmBI CGTCTC 1 cut(s) 3032
BsmFI GGGAC 7 cut(s) 430, 1362, 2544, 3121, 3139, 3689, 3857
BsnI GGCC 7 cut(s) 103, 207, 1911, 2581, 3722, 3932, 4045
Bso31I GGTCTC 3 cut(s) 152, 471, 3373
BsoBI CYCGRG 1 cut(s) 468
Bsp120I GGGCCC 1 cut(s) 1909
Bsp1286I GDGCHC 2 cut(s) 1204, 1913
Bsp1407I TGTACA 3 cut(s) 580, 883, 1291
Bsp68I TCGCGA 1 cut(s) 131
BspANI GGCC 7 cut(s) 103, 207, 1911, 2581, 3722, 3932, 4045
BspCNI CTCAG 7 cut(s) 61, 148, 328, 1616, 2929, 3044, 3394
BspDI ATCGAT 2 cut(s) 2283, 4050
BspFNI CGCG 3 cut(s) 40, 131, 413
BspHI TCATGA 1 cut(s) 1567
BspLI GGNNCC 4 cut(s) 36, 1911, 2847, 3723
BspMAI CTGCAG 1 cut(s) 850
BspMI ACCTGC 1 cut(s) 4142
BspOI GCTAGC 1 cut(s) 3569
BspPI GGATC 5 cut(s) 2288, 2534, 2723, 3110, 3585
BspT107I GGYRCC 2 cut(s) 34, 2845
BspTNI GGTCTC 3 cut(s) 152, 471, 3373
BsrBI CCGCTC 2 cut(s) 278, 1177
BsrDI GCAATG 3 cut(s) 159, 239, 3318
BsrFI RCCGGY 1 cut(s) 1332
BsrGI TGTACA 3 cut(s) 580, 883, 1291
BsrI ACTGG 5 cut(s) 591, 2452, 2582, 3368, 3450
BssAI RCCGGY 1 cut(s) 1332
BssECI CCNNGG 7 cut(s) 98, 202, 292, 411, 2460, 2792, 3413
BssNI GRCGYC 1 cut(s) 114
BssT1I CCWWGG 3 cut(s) 98, 2792, 3413
Bst2UI CCWGG 6 cut(s) 294, 339, 936, 1557, 1620, 3571
Bst6I CTCTTC 3 cut(s) 24, 1989, 2370
BstACI GRCGYC 1 cut(s) 114
BstAPI GCANNNNNTGC 1 cut(s) 1199
BstAUI TGTACA 3 cut(s) 580, 883, 1291
BstBAI YACGTR 1 cut(s) 2918
BstC8I GCNNGC 8 cut(s) 129, 850, 997, 1387, 2023, 2262, 3567, 4169
BstDSI CCRYGG 1 cut(s) 411
BstEII GGTNACC 4 cut(s) 360, 624, 1064, 1335
BstENI CCTNNNNNAGG 1 cut(s) 1470
BstFNI CGCG 3 cut(s) 40, 131, 413
BstMAI GTCTC 8 cut(s) 152, 313, 471, 705, 879, 2390, 3032, 3373
BstMCI CGRYCG 1 cut(s) 208
BstMWI GCNNNNNNNGC 6 cut(s) 100, 1031, 1199, 1208, 3674, 4157
BstNI CCWGG 6 cut(s) 294, 339, 936, 1557, 1620, 3571
BstNSI RCATGY 1 cut(s) 2264
BstPAI GACNNNNGTC 1 cut(s) 3172
BstPI GGTNACC 4 cut(s) 360, 624, 1064, 1335
BstSCI CCNGG 8 cut(s) 292, 337, 934, 1202, 1555, 1618, 3202, 3569
BstSFI CTRYAG 4 cut(s) 846, 1608, 1943, 3193
BstSLI GKGCMC 2 cut(s) 1204, 1913
BstUI CGCG 3 cut(s) 40, 131, 413
BstV1I GCAGC 9 cut(s) 136, 832, 1009, 1369, 1531, 2201, 2933, 3563, 3869
BstV2I GAAGAC 2 cut(s) 1827, 3825
BstX2I RGATCY 4 cut(s) 1717, 2728, 3481, 3490
BstYI RGATCY 4 cut(s) 1717, 2728, 3481, 3490
BstZI CGGCCG 1 cut(s) 205
Bsu15I ATCGAT 2 cut(s) 2283, 4050
BsuI GTATCC 2 cut(s) 2417, 4222
BsuRI GGCC 7 cut(s) 103, 207, 1911, 2581, 3722, 3932, 4045
BsuTUI ATCGAT 2 cut(s) 2283, 4050
BtgI CCRYGG 1 cut(s) 411
BtrI CACGTC 1 cut(s) 1864
BtsI GCAGTG 1 cut(s) 2647
BtsIMutI CAGTG 2 cut(s) 2624, 2647
BtuMI TCGCGA 1 cut(s) 131
BveI ACCTGC 1 cut(s) 4142
Cac8I GCNNGC 8 cut(s) 129, 850, 997, 1387, 2023, 2262, 3567, 4169
CciI TCATGA 1 cut(s) 1567
Cfr10I RCCGGY 1 cut(s) 1332
Cfr13I GGNCC 5 cut(s) 1599, 1909, 1910, 3308, 3721
Cfr42I CCGCGG 1 cut(s) 414
ClaI ATCGAT 2 cut(s) 2283, 4050
CseI GACGC 4 cut(s) 29, 116, 122, 1863
Csp6I GTAC 9 cut(s) 581, 884, 1292, 2380, 2846, 3185, 3397, 3916, 4203
CspAI ACCGGT 1 cut(s) 1332
CspCI CAANNNNNGTGG 2 cut(s) 2095, 2130
CviQI GTAC 9 cut(s) 581, 884, 1292, 2380, 2846, 3185, 3397, 3916, 4203
DraI TTTAAA 1 cut(s) 3132
DraIII CACNNNGTG 1 cut(s) 2619
DrdI GACNNNNNNGTC 1 cut(s) 2204
DseDI GACNNNNNNGTC 1 cut(s) 2204
EaeI YGGCCR 2 cut(s) 101, 205
EagI CGGCCG 1 cut(s) 205
Eam1104I CTCTTC 3 cut(s) 24, 1989, 2370
EarI CTCTTC 3 cut(s) 24, 1989, 2370
EclXI CGGCCG 1 cut(s) 205
Eco130I CCWWGG 3 cut(s) 98, 2792, 3413
Eco24I GRGCYC 1 cut(s) 1913
Eco31I GGTCTC 3 cut(s) 152, 471, 3373
Eco32I GATATC 2 cut(s) 2090, 3200
Eco47I GGWCC 2 cut(s) 1599, 3308
Eco52I CGGCCG 1 cut(s) 205
Eco57I CTGAAG 5 cut(s) 185, 313, 657, 3179, 3498
Eco72I CACGTG 1 cut(s) 2918
Eco88I CYCGRG 1 cut(s) 468
Eco91I GGTNACC 4 cut(s) 360, 624, 1064, 1335
EcoNI CCTNNNNNAGG 1 cut(s) 1470
EcoO109I RGGNCCY 3 cut(s) 1599, 1909, 3721
EcoO65I GGTNACC 4 cut(s) 360, 624, 1064, 1335
EcoRI GAATTC 1 cut(s) 1664
EcoRII CCWGG 6 cut(s) 292, 337, 934, 1555, 1618, 3569
EcoRV GATATC 2 cut(s) 2090, 3200
EcoT14I CCWWGG 3 cut(s) 98, 2792, 3413
EcoT22I ATGCAT 5 cut(s) 613, 787, 1811, 2262, 2838
EcoT38I GRGCYC 1 cut(s) 1913
ErhI CCWWGG 3 cut(s) 98, 2792, 3413
Esp3I CGTCTC 1 cut(s) 3032
FaqI GGGAC 7 cut(s) 430, 1362, 2544, 3121, 3139, 3689, 3857
FauI CCCGC 1 cut(s) 406
FauNDI CATATG 1 cut(s) 1486
FbaI TGATCA 1 cut(s) 1456
FblI GTMKAC 4 cut(s) 43, 1154, 1283, 3192
FriOI GRGCYC 1 cut(s) 1913
GsaI CCCAGC 1 cut(s) 1022
GsuI CTGGAG 3 cut(s) 321, 1641, 1719
HaeIII GGCC 7 cut(s) 103, 207, 1911, 2581, 3722, 3932, 4045
HapII CCGG 6 cut(s) 70, 104, 1204, 1333, 2849, 3203
HgaI GACGC 4 cut(s) 29, 116, 122, 1863
Hin1I GRCGYC 1 cut(s) 114
HincII GTYRAC 1 cut(s) 1882
HindII GTYRAC 1 cut(s) 1882
HindIII AAGCTT 3 cut(s) 669, 2880, 3423
HpaII CCGG 6 cut(s) 70, 104, 1204, 1333, 2849, 3203
Hpy99I CGWCG 2 cut(s) 256, 1830
HpyF10VI GCNNNNNNNGC 6 cut(s) 100, 1031, 1199, 1208, 3674, 4157
Hsp92I GRCGYC 1 cut(s) 114
KpnI GGTACC 1 cut(s) 2849
Ksp22I TGATCA 1 cut(s) 1456
KspI CCGCGG 1 cut(s) 414
LmnI GCTCC 7 cut(s) 72, 580, 754, 1039, 1417, 1944, 2682
Lsp1109I GCAGC 9 cut(s) 136, 832, 1009, 1369, 1531, 2201, 2933, 3563, 3869
LweI GCATC 9 cut(s) 198, 598, 772, 1818, 2176, 2881, 3664, 3817, 3937
MbiI CCGCTC 2 cut(s) 278, 1177
MfeI CAATTG 1 cut(s) 1220
MflI RGATCY 4 cut(s) 1717, 2728, 3481, 3490
MhlI GDGCHC 2 cut(s) 1204, 1913
MlyI GAGTC 3 cut(s) 127, 561, 3466
MmeI TCCRAC 6 cut(s) 613, 781, 1195, 2756, 3143, 3250
Mph1103I ATGCAT 5 cut(s) 613, 787, 1811, 2262, 2838
MroXI GAANNNNTTC 4 cut(s) 771, 1575, 2067, 3492
MslI CAYNNNNRTG 3 cut(s) 2045, 2415, 2439
MspA1I CMGCKG 7 cut(s) 413, 490, 1022, 1211, 3113, 3172, 3554
MspI CCGG 6 cut(s) 70, 104, 1204, 1333, 2849, 3203
MspR9I CCNGG 8 cut(s) 294, 339, 936, 1204, 1557, 1620, 3204, 3571
MunI CAATTG 1 cut(s) 1220
MvaI CCWGG 6 cut(s) 294, 339, 936, 1557, 1620, 3571
MvnI CGCG 3 cut(s) 40, 131, 413
MwoI GCNNNNNNNGC 6 cut(s) 100, 1031, 1199, 1208, 3674, 4157
NciI CCSGG 2 cut(s) 1204, 3204
NdeI CATATG 1 cut(s) 1486
NheI GCTAGC 1 cut(s) 3565
NlaIV GGNNCC 4 cut(s) 36, 1911, 2847, 3723
NmeAIII GCCGAG 1 cut(s) 183
NruI TCGCGA 1 cut(s) 131
NsiI ATGCAT 5 cut(s) 613, 787, 1811, 2262, 2838
NspI RCATGY 1 cut(s) 2264
PaeI GCATGC 1 cut(s) 2264
PaeR7I CTCGAG 1 cut(s) 468
PagI TCATGA 1 cut(s) 1567
PcsI WCGNNNNNNNCGW 1 cut(s) 2807
PdmI GAANNNNTTC 4 cut(s) 771, 1575, 2067, 3492
PfeI GAWTC 9 cut(s) 1847, 2068, 2393, 2451, 2698, 3008, 3089, 3260, 4006
PflMI CCANNNNNTGG 2 cut(s) 2884, 3742
PfoI TCCNGGA 1 cut(s) 1555
PinAI ACCGGT 1 cut(s) 1332
PleI GAGTC 3 cut(s) 127, 561, 3465
PmaCI CACGTG 1 cut(s) 2918
PmlI CACGTG 1 cut(s) 2918
PpsI GAGTC 3 cut(s) 127, 561, 3465
Ppu21I YACGTR 1 cut(s) 2918
PpuMI RGGWCCY 1 cut(s) 1599
PshAI GACNNNNGTC 1 cut(s) 3172
PshBI ATTAAT 2 cut(s) 2978, 3785
PsiI TTATAA 1 cut(s) 3792
Psp1406I AACGTT 1 cut(s) 2657
Psp5II RGGWCCY 1 cut(s) 1599
Psp6I CCWGG 6 cut(s) 292, 337, 934, 1555, 1618, 3569
PspCI CACGTG 1 cut(s) 2918
PspEI GGTNACC 4 cut(s) 360, 624, 1064, 1335
PspFI CCCAGC 1 cut(s) 1018
PspGI CCWGG 6 cut(s) 292, 337, 934, 1555, 1618, 3569
PspN4I GGNNCC 4 cut(s) 36, 1911, 2847, 3723
PspOMI GGGCCC 1 cut(s) 1909
PspPI GGNCC 5 cut(s) 1599, 1909, 1910, 3308, 3721
PspPPI RGGWCCY 1 cut(s) 1599
PsrI GAACNNNNNNTAC 2 cut(s) 3743, 3775
PstI CTGCAG 1 cut(s) 850
PsuI RGATCY 4 cut(s) 1717, 2728, 3481, 3490
PvuII CAGCTG 6 cut(s) 490, 1022, 1211, 3113, 3172, 3554
RruI TCGCGA 1 cut(s) 131
RsaI GTAC 9 cut(s) 582, 885, 1293, 2381, 2847, 3186, 3398, 3917, 4204
RsaNI GTAC 9 cut(s) 581, 884, 1292, 2380, 2846, 3185, 3397, 3916, 4203
RseI CAYNNNNRTG 3 cut(s) 2045, 2415, 2439
SacII CCGCGG 1 cut(s) 414
Sau96I GGNCC 5 cut(s) 1599, 1909, 1910, 3308, 3721
ScaI AGTACT 1 cut(s) 2381
SchI GAGTC 3 cut(s) 127, 561, 3466
ScrFI CCNGG 8 cut(s) 294, 339, 936, 1204, 1557, 1620, 3204, 3571
SduI GDGCHC 2 cut(s) 1204, 1913
SfaNI GCATC 9 cut(s) 198, 598, 772, 1818, 2176, 2881, 3664, 3817, 3937
SfcI CTRYAG 4 cut(s) 846, 1608, 1943, 3193
Sfr274I CTCGAG 1 cut(s) 468
Sfr303I CCGCGG 1 cut(s) 414
SgrBI CCGCGG 1 cut(s) 414
SinI GGWCC 2 cut(s) 1599, 3308
SlaI CTCGAG 1 cut(s) 468
SmiMI CAYNNNNRTG 3 cut(s) 2045, 2415, 2439
SmlI CTYRAG 3 cut(s) 468, 2552, 3370
SmoI CTYRAG 3 cut(s) 468, 2552, 3370
SpeI ACTAGT 1 cut(s) 3187
SphI GCATGC 1 cut(s) 2264
SspI AATATT 1 cut(s) 1754
StyD4I CCNGG 8 cut(s) 292, 337, 934, 1202, 1555, 1618, 3202, 3569
StyI CCWWGG 3 cut(s) 98, 2792, 3413
TaqII GACCGA 3 cut(s) 612, 786, 1420
TatI WGTACW 5 cut(s) 580, 883, 1291, 2379, 3915
TauI GCSGC 6 cut(s) 40, 152, 413, 545, 1730, 2086
TfiI GAWTC 9 cut(s) 1847, 2068, 2393, 2451, 2698, 3008, 3089, 3260, 4006
TscAI CASTG 2 cut(s) 2624, 2647
TseI GCWGC 9 cut(s) 124, 845, 1022, 1382, 1544, 2189, 2921, 3551, 3882
TspGWI ACGGA 3 cut(s) 240, 1658, 3763
TspRI CASTG 2 cut(s) 2624, 2647
Van91I CCANNNNNTGG 2 cut(s) 2884, 3742
VpaK11BI GGWCC 2 cut(s) 1599, 3308
VspI ATTAAT 2 cut(s) 2978, 3785
XagI CCTNNNNNAGG 1 cut(s) 1470
XapI RAATTY 4 cut(s) 1664, 1782, 1834, 2855
XbaI TCTAGA 4 cut(s) 925, 3001, 3484, 3670
XceI RCATGY 1 cut(s) 2264
XcmI CCANNNNNNNNNTGG 1 cut(s) 3411
XhoI CTCGAG 1 cut(s) 468
XmaJI CCTAGG 1 cut(s) 2792
XmiI GTMKAC 4 cut(s) 43, 1154, 1283, 3192
XmnI GAANNNNTTC 4 cut(s) 771, 1575, 2067, 3492
ZrmI AGTACT 1 cut(s) 2381
Zsp2I ATGCAT 5 cut(s) 613, 787, 1811, 2262, 2838
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.