RLG00000016242
ERF Family

Probably involved in the RNA silencing pathway and required for the generation of small interfering RNAs (siRNAs)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0000505
Physical Location & Seq
Forward (+)
5021068 .. 5031618
10551 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000016242

Sequence Viewer

Length: 3000 bp
ATGGAAGAAGTGAGATTACCTCCGCCGGTGGAGGAGTTGCTCCGTAGAATCTGTACTGAGAAAAAGCTACCATGGCCGGACTCCGGGGCCCGGCGGAAGCTGGCCTCTATCAGCCAGGAACGTGCACATGAGGTCCTGGTTAAAGTCTTCAACAAACAAAATATCATAAGTCTCGGCGGTTTCATCAACTACTTGATCCATAACCTCCCTGATTCTCCTCCTCCTTCGACTGCTGTCCGCGCTTATTATGATCAAAGGCCCTCTGCTTGTCCAGTTACTCCACCTTGTTCTCAACTTCAGTACAGCTCCGCAGTTGCAGGTCAGGCATTCAGTGATCGGACCGAGCAGCCTGTGATTAATGGACGGTATGGACCATCACCATATTCTTCTGGTTGTAAAGCCATCAGCTCTTTCTCGTATCCAACAATACCGCTGGCTTGTAACATTGCTCCAGTGCAACTTGATCATAGGTTTCAAGGTGAGGTTGTAACTAAGTCGCCAGATCTAGAGAAATATGGAGGCCATGGACAAGCACACTTGGCAGCTCTTGGTACTCTTGAATTCAGAAAACAATTTCTGATCTTGAGCTACATTAGAGAGGAAAAGTTAAAGAATGTTATAGTTGCAGAGGATATTAAGCATTTGAAAGATTTACCAATGGCAGAGTTTGAGTCCAAAGTTTGGCTACTCTTCGGACATAAATATATCAGCAATGGAGACCGACAGTTGAATTTGGATTGGGATGGCAGACGCGCACATTTCTATGAGTGCCATGTTACCAGAGATGGGAGTTACACATTCAAGGGCCTCTATCTACAAAATGAAAGAAGAACTCTCCTACAGAAACTATGTCAAGATGACAATGTTTTGATTGTGAAGTTTGACGAAGAAGGGACACTGACAGGTTCCAGGACTTCTTATGATAATGACTATTCTACGTATATGAAGGTTGAGAGACAAGGGATTTTTGTTGGCTTGCGGCCCTATCATTTCTTTGGTTTTAAAGATGGTGGTAAAAGAGATAAAATCAGAAGTCCCACATCATCACCCGTAAAATGTTATTTTGTTCGTGTGAGCTCTGATGCGGTAATAGACAGGGATGTAGGTTATTGGTTATCTAACAGAACAATTCATGAAGGAAGACGTCTAATTATGCATGTTGATACTCTGCCAAGAGTGGACAAGGTCATGGCTAGGTTGCAACTCATTTTTTCTAAGACAACGACCTTGGAAGTAGATTGGTCACTTGTGAAGGTTGAAGAAATTGTTGACGTCAACTGTTGCATATGGATGAATCTGATAGAAGCCAAAAGAAAGTTTATGAGTGAGGAACACATGGAGGGACTTCCGGATCCTGATGAACTTGAGATTGAAATGAAAAAATCAGATCTCCGAACTCAAGAACCGCCATTGCTCATACAGTGCCGCCTATTTCATGAAGGCAATGCTTATAACGGAACTTTGCTTGTCAATAAACGGCTTCCCCCGAAAACACTGCAGATTCGACCGTCAATGCATAAAGTTGAGAAGGACCCTAAGATTTCAGATAATGAAACCATCAATTCTTTGGAAATAGTTTGCACAAGTGCTCCTCCTAGGAAAGCTTATTTATCAAGGATTATAATTGCACTTCTAGTGCGTGGAGGTGTACCGAGAGACTTCTTTATGAAGATACTTGACAATGCTTTAGAAGATGCTCATCGAGTTATCGGGAAGACACGTGCAGCACTCAAAGCAATTTCGTATTTTTTATGTTCCACATTTTTTGTGGATTTTGTGCCTACAATTGCTGAGAAGTATGGTGAGATTGATGACAATTTCTTGGGAGCAACAATGATCAGACTCGGTATTCCTCTAGAAGAATCATATTTGCAATATCGTCTGTCACACTTAATGCGTGTGCAGGAAAGCGCTGGTCTTAGAAAAGGGAGACTTCATATTCCTGACTCTTATGTGTTGATGGGGACAGCTGATCCAACAGGGATTCTGGAAAGAGATGAAGTCTGCGTTATCCTTAAGGACGGACAAATGTCGGGGAAGGTACTTGTGTACCGATATCCTGGTTTACACTTCGGGGATATTAAAGTTTTGAAGGCCACCTATGTGAAGGAGTTAGAATCCATTGTGGGAGATGCCAGATATGCTATATTCTTCTCTTGCAAGGGACCACGTAATGTTGCTGACGAAATTGGTGGTGGAGATTTTGATGGTGATCTTTACTGGGTCTCAAGAAATCCTCAGCTTTCCACTGAAGATCTTGAAGCTGATATGTTTAGATTGTTCTTGAAAAATAGGTTTCAACCAAGTTTTGCTATGAGTGAGGCAGCTAATAACTGGCTAGCTTGGATGGATTGGTTCTTGACCTTGGGAGATAGTAATGAGAAGAAGGACGTAATGGACAAGATACTGAAGTTGGTTGATATATATTACATAGCTTTAGATGCAGCCAAGAAGGGTGTAAAGGTTGAAGTTCCAAAAGATTTGAAGGTGTCACTTTTCCCTCATTACATGGACAAGAAAAAAGAAGAAGAAACTTACATTTCATCTTCGATTTTGGGAGAAATTTATGATGAAGTTGATAAATGTCAAGCAAAAATAATTTTTCTCAGAGAAATCAAGAAACTTCCTACTTTTGATATTAATCAAGTCCCCGAACTCTGCTTGGCCAAATGGAAGAAACGTTATGGACGGTACAGGACTGAAATGAGCTCGGCCATGGATGAGGATGGAGATCAGGCTGCCATTGTCATAAGAAAGTATAAAAAGAAGCTGTATAAGGCTGATGACTTTGAGCACAGTTCAAGGCCGATGGATGAAATACTGGACGAGGCACACGCTATATATCATGTATGTTATGACCATGCTATGTATAAAAGAGATGTGAAAAAATGTTCTTTTGCATGGAGAGTTGCAGGTAAAGCTCTGTGCGAGCTTCACGCTAGGAAAAAGGATGAGAAGTGGTTAAACATTGCCCCTTCTGTTTTGAAAGAGCTTTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RdRP

Protein Analysis

1000

Amino Acids

114.53

Weight (kDa)

8.16

Isoelectric Point (pI)

44.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
4HB_RdRP3_N PF26249 5 - 67 1.9e-08 RdRP3/4/5, N-terminal four helical bundle domain
RdRP_helical PF26252 179 - 245 2.9e-09 RNA dependent RNA polymerase, helical domain
RdRP PF05183 266 - 859 1.3e-88 RNA dependent RNA polymerase
RdRP_head PF26253 911 - 984 1.3e-11 RdRP, head domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000505)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G19910 AT2G19910 AT2G19920 AT2G19920 AT2G19920 AT2G19920 AT2G19930 AT2G19930 AT2G19930
fragaria_vesca FvH4_1g05980 FvH4_1g05990 FvH4_1g05990 FvH4_1g09718
malus_domestica MD02G1064400.v1.1 MD15G1195300.v1.1 MD15G1195400.v1.1
prunus_persica Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1
pyrus_communis pycom02g05070 pycom15g17460
rosa_chinensis RchiOBHm_Chr2g0091671 RchiOBHm_Chr2g0091681 RchiOBHm_Chr2g0091691 RchiOBHm_Chr2g0091701 RchiOBHm_Chr2g0091711 RchiOBHm_Chr2g0091721 RchiOBHm_Chr2g0091731 RchiOBHm_Chr5g0014401
rosa_laevigata RLG00000013772 RLG00000016242 RLG00000016244 RLG00000016245 RLG00000016246 RLG00000032105
rosa_multiflora Rmu_co8374041.1_g000001 Rmu_co8478121.1_g000001 Rmu_sc0000897.1_g000005 Rmu_sc0000897.1_g000009
rosa_roxburghii Rroxscaffold_1G00062180 Rroxscaffold_1G00062190 Rroxscaffold_1G00068800 Rroxscaffold_2G00149790 Rroxscaffold_2G00149800 Rroxscaffold_2G00149810
rosa_rugosa Rorug02G0019600 Rorug02G0019700 Rorug02G0019800 Rorug02G0019900 Rorug02G0019900 Rorug02G0020000 Rorug02G0020000 Rorug02G0020100 Rorug02G0020100 Rorug02G0020200 Rorug05G0017800
rosa_samantha Rh2AG064700 Rh2AG064800 Rh2AG064900 Rh2BG063800 Rh2BG063900 Rh2BG064000 Rh2BG064100 Rh2CG065500 Rh2CG065600 Rh2CG065700 Rh2DG063900 Rh2DG064000 Rh2DG064100 Rh2DG064200 Rh2DG381700 Rh5AG056700 Rh5AG056800 Rh5AG056900 Rh5AG112600 Rh5BG109300 Rh5CG063800 Rh5CG121100 Rh5DG053100 Rh5DG108300 Rh6CG100100
rosa_wichuraiana Rw2G005560 Rw2G005580 Rw2G005590 Rw5G005370 Rw5G009770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1452, 1622
AatII GACGTC 2 cut(s) 1147, 1275
Acc36I ACCTGC 2 cut(s) 308, 2903
AccB7I CCANNNNNTGG 1 cut(s) 681
AccII CGCG 2 cut(s) 240, 753
AccIII TCCGGA 1 cut(s) 1348
AclI AACGTT 1 cut(s) 2680
AclWI GGATC 4 cut(s) 190, 1346, 1359, 1967
AcoI YGGCCR 3 cut(s) 74, 2664, 2712
AcsI RAATTY 3 cut(s) 560, 730, 2562
AcuI CTGAAG 3 cut(s) 281, 2271, 2429
AcvI CACGTG 1 cut(s) 1721
AcyI GRCGYC 2 cut(s) 1144, 1272
AfaI GTAC 7 cut(s) 55, 302, 553, 1650, 2043, 2051, 2693
AfeI AGCGCT 1 cut(s) 1912
AfiI CCNNNNNNNGG 4 cut(s) 83, 90, 681, 786
AflII CTTAAG 1 cut(s) 2015
AflIII ACRYGT 1 cut(s) 1718
AjnI CCWGG 4 cut(s) 114, 135, 908, 2059
AleI CACNNNNGTG 1 cut(s) 2102
Alw21I GWGCWC 5 cut(s) 127, 1079, 1591, 2711, 2796
Alw26I GTCTC 6 cut(s) 176, 711, 949, 1650, 1924, 2230
Alw44I GTGCAC 1 cut(s) 123
AlwI GGATC 4 cut(s) 190, 1346, 1359, 1967
Aor13HI TCCGGA 1 cut(s) 1348
Aor51HI AGCGCT 1 cut(s) 1912
ApaI GGGCCC 1 cut(s) 91
ApaLI GTGCAC 1 cut(s) 123
ApeKI GCWGC 6 cut(s) 346, 542, 1724, 2324, 2444, 2738
ApoI RAATTY 3 cut(s) 560, 730, 2562
AseI ATTAAT 2 cut(s) 357, 2640
AspA2I CCTAGG 1 cut(s) 1595
AspLEI GCGC 3 cut(s) 242, 755, 1913
AsuC2I CCSGG 2 cut(s) 85, 91
AsuHPI GGTGA 5 cut(s) 369, 491, 1038, 1814, 2222
AsuNHI GCTAGC 1 cut(s) 2338
AvaII GGWCC 5 cut(s) 133, 339, 371, 1531, 2165
AvrII CCTAGG 1 cut(s) 1595
BaeGI GKGCMC 2 cut(s) 91, 127
BaeI ACNNNNGTAYC 6 cut(s) 2033, 2033, 2066, 2066, 2396, 2429
BalI TGGCCA 1 cut(s) 2666
BamHI GGATCC 1 cut(s) 1351
BanII GRGCYC 3 cut(s) 91, 1079, 2711
BarI GAAGNNNNNNTAC 1 cut(s) 32
BbrPI CACGTG 1 cut(s) 1721
BbsI GAAGAC 3 cut(s) 139, 1147, 1721
Bbv12I GWGCWC 5 cut(s) 127, 1079, 1591, 2711, 2796
BbvCI CCTCAGC 1 cut(s) 2238
BbvI GCAGC 6 cut(s) 358, 554, 1736, 2336, 2456, 2725
BceAI ACGGC 1 cut(s) 1493
BcgI CGANNNNNNTGC 2 cut(s) 1477, 1511
BciT130I CCWGG 4 cut(s) 116, 137, 910, 2061
BciVI GTATCC 1 cut(s) 429
BclI TGATCA 3 cut(s) 250, 463, 1836
BcnI CCSGG 2 cut(s) 85, 91
BcoDI GTCTC 6 cut(s) 176, 711, 949, 1650, 1924, 2230
BfaI CTAG 7 cut(s) 506, 1194, 1596, 1634, 1856, 2339, 2940
BfmI CTRYAG 2 cut(s) 839, 1496
BfoI RGCGCY 1 cut(s) 1914
BfrI CTTAAG 1 cut(s) 2015
BfuAI ACCTGC 2 cut(s) 308, 2903
BfuI GTATCC 1 cut(s) 429
BglII AGATCT 3 cut(s) 502, 1387, 2254
BisI GCNGC 8 cut(s) 347, 543, 980, 1426, 1725, 2325, 2445, 2739
BlnI CCTAGG 1 cut(s) 1595
BlsI GCNGC 8 cut(s) 348, 544, 981, 1427, 1726, 2326, 2446, 2740
Bme1390I CCNGG 6 cut(s) 85, 91, 116, 137, 910, 2061
Bme18I GGWCC 5 cut(s) 133, 339, 371, 1531, 2165
BmiI GGNNCC 6 cut(s) 88, 89, 907, 1353, 1533, 2166
BmrFI CCNGG 6 cut(s) 85, 91, 116, 137, 910, 2061
BmrI ACTGGG 1 cut(s) 2230
BmsI GCATC 4 cut(s) 1072, 1684, 2122, 2431
BmtI GCTAGC 1 cut(s) 2342
BmuI ACTGGG 1 cut(s) 2230
BoxI GACNNNNGTC 2 cut(s) 233, 2029
BpiI GAAGAC 3 cut(s) 139, 1147, 1721
BplI GAGNNNNNCTC 1 cut(s) 36
BpmI CTGGAG 1 cut(s) 435
Bpu10I CCTNAGC 1 cut(s) 2238
BpuEI CTTGAG 4 cut(s) 604, 1383, 1385, 2212
BpuMI CCSGG 2 cut(s) 85, 91
BsaAI YACGTR 3 cut(s) 939, 1721, 2171
BsaBI GATNNNNATC 4 cut(s) 1698, 2211, 2640, 2730
BsaHI GRCGYC 2 cut(s) 1144, 1272
BsaI GGTCTC 2 cut(s) 711, 2230
BsaJI CCNNGG 7 cut(s) 71, 84, 523, 1227, 1595, 2364, 2715
BsaWI WCCGGW 1 cut(s) 1348
BsaXI ACNNNNNCTCC 2 cut(s) 1573, 1603
Bsc4I CCNNNNNNNGG 4 cut(s) 83, 90, 681, 786
Bse118I RCCGGY 1 cut(s) 25
Bse1I ACTGG 5 cut(s) 272, 452, 2225, 2339, 2826
Bse3DI GCAATG 5 cut(s) 444, 718, 1409, 1450, 2967
Bse8I GATNNNNATC 4 cut(s) 1698, 2211, 2640, 2730
BseAI TCCGGA 1 cut(s) 1348
BseBI CCWGG 4 cut(s) 116, 137, 910, 2061
BseDI CCNNGG 7 cut(s) 71, 84, 523, 1227, 1595, 2364, 2715
BseGI GGATG 8 cut(s) 748, 1105, 1296, 2352, 2725, 2731, 2818, 2956
BseJI GATNNNNATC 4 cut(s) 1698, 2211, 2640, 2730
BseLI CCNNNNNNNGG 4 cut(s) 83, 90, 681, 786
BseMI GCAATG 5 cut(s) 444, 718, 1409, 1450, 2967
BseMII CTCAG 4 cut(s) 48, 1782, 2252, 2620
BseNI ACTGG 5 cut(s) 272, 452, 2225, 2339, 2826
BseRI GAGGAG 4 cut(s) 47, 207, 210, 1581
BseSI GKGCMC 2 cut(s) 91, 127
BseXI GCAGC 6 cut(s) 358, 554, 1736, 2336, 2456, 2725
BsgI GTGCAG 2 cut(s) 1743, 1922
Bsh1236I CGCG 2 cut(s) 240, 753
Bsh1285I CGRYCG 1 cut(s) 1508
BsiEI CGRYCG 1 cut(s) 1508
BsiHKAI GWGCWC 5 cut(s) 127, 1079, 1591, 2711, 2796
BsiSI CCGG 5 cut(s) 26, 77, 84, 91, 1349
BslFI GGGAC 6 cut(s) 907, 1020, 1356, 1978, 2178, 2633
BslI CCNNNNNNNGG 4 cut(s) 83, 90, 681, 786
BsmAI GTCTC 6 cut(s) 176, 711, 949, 1650, 1924, 2230
BsmFI GGGAC 6 cut(s) 907, 1020, 1356, 1978, 2178, 2633
BsmI GAATGC 1 cut(s) 326
Bso31I GGTCTC 2 cut(s) 711, 2230
Bsp120I GGGCCC 1 cut(s) 87
Bsp1286I GDGCHC 6 cut(s) 91, 127, 1079, 1591, 2711, 2796
Bsp13I TCCGGA 1 cut(s) 1348
Bsp19I CCATGG 3 cut(s) 71, 523, 2715
BspCNI CTCAG 4 cut(s) 49, 1783, 2251, 2619
BspEI TCCGGA 1 cut(s) 1348
BspFNI CGCG 2 cut(s) 240, 753
BspHI TCATGA 2 cut(s) 1132, 1435
BspLI GGNNCC 6 cut(s) 88, 89, 907, 1353, 1533, 2166
BspMAI CTGCAG 1 cut(s) 1500
BspMI ACCTGC 2 cut(s) 308, 2903
BspOI GCTAGC 1 cut(s) 2342
BspPI GGATC 4 cut(s) 190, 1346, 1359, 1967
BspTI CTTAAG 1 cut(s) 2015
BspTNI GGTCTC 2 cut(s) 711, 2230
BsrDI GCAATG 5 cut(s) 444, 718, 1409, 1450, 2967
BsrFI RCCGGY 1 cut(s) 25
BsrI ACTGG 5 cut(s) 272, 452, 2225, 2339, 2826
BssAI RCCGGY 1 cut(s) 25
BssECI CCNNGG 7 cut(s) 71, 84, 523, 1227, 1595, 2364, 2715
BssNI GRCGYC 2 cut(s) 1144, 1272
BssT1I CCWWGG 6 cut(s) 71, 523, 1227, 1595, 2364, 2715
Bst2UI CCWGG 4 cut(s) 116, 137, 910, 2061
Bst4CI ACNGT 7 cut(s) 366, 726, 1280, 1422, 1509, 2691, 2798
Bst6I CTCTTC 1 cut(s) 695
BstACI GRCGYC 2 cut(s) 1144, 1272
BstAFI CTTAAG 1 cut(s) 2015
BstBAI YACGTR 3 cut(s) 939, 1721, 2171
BstC8I GCNNGC 5 cut(s) 102, 435, 977, 2340, 2930
BstDEI CTNAG 8 cut(s) 57, 492, 1215, 1536, 1791, 1919, 2238, 2606
BstDSI CCRYGG 3 cut(s) 71, 523, 2715
BstF5I GGATG 8 cut(s) 748, 1105, 1296, 2352, 2725, 2731, 2818, 2956
BstFNI CGCG 2 cut(s) 240, 753
BstH2I RGCGCY 1 cut(s) 1914
BstHHI GCGC 3 cut(s) 242, 755, 1913
BstMAI GTCTC 6 cut(s) 176, 711, 949, 1650, 1924, 2230
BstMCI CGRYCG 1 cut(s) 1508
BstMWI GCNNNNNNNGC 8 cut(s) 73, 239, 323, 539, 1733, 2141, 2441, 2918
BstNI CCWGG 4 cut(s) 116, 137, 910, 2061
BstNSI RCATGY 1 cut(s) 1160
BstPAI GACNNNNGTC 2 cut(s) 233, 2029
BstSCI CCNGG 6 cut(s) 83, 89, 114, 135, 908, 2059
BstSFI CTRYAG 2 cut(s) 839, 1496
BstSLI GKGCMC 2 cut(s) 91, 127
BstSNI TACGTA 1 cut(s) 939
BstUI CGCG 2 cut(s) 240, 753
BstV1I GCAGC 6 cut(s) 358, 554, 1736, 2336, 2456, 2725
BstV2I GAAGAC 3 cut(s) 139, 1147, 1721
BstX2I RGATCY 4 cut(s) 502, 1351, 1387, 2254
BstYI RGATCY 4 cut(s) 502, 1351, 1387, 2254
BsuI GTATCC 1 cut(s) 429
BtgI CCRYGG 3 cut(s) 71, 523, 2715
BtsCI GGATG 8 cut(s) 748, 1105, 1296, 2352, 2725, 2731, 2818, 2956
BtsI GCAGTG 1 cut(s) 1493
BtsIMutI CAGTG 6 cut(s) 337, 459, 896, 1427, 1493, 2247
BveI ACCTGC 2 cut(s) 308, 2903
Cac8I GCNNGC 5 cut(s) 102, 435, 977, 2340, 2930
CciI TCATGA 2 cut(s) 1132, 1435
CfoI GCGC 3 cut(s) 242, 755, 1913
Cfr10I RCCGGY 1 cut(s) 25
CpoI CGGWCCG 1 cut(s) 339
CseI GACGC 1 cut(s) 759
Csp6I GTAC 7 cut(s) 54, 301, 552, 1649, 2042, 2050, 2692
CspCI CAANNNNNGTGG 2 cut(s) 1747, 1782
CspI CGGWCCG 1 cut(s) 339
CviQI GTAC 7 cut(s) 54, 301, 552, 1649, 2042, 2050, 2692
DdeI CTNAG 8 cut(s) 57, 492, 1215, 1536, 1791, 1919, 2238, 2606
DraI TTTAAA 1 cut(s) 1003
EaeI YGGCCR 3 cut(s) 74, 2664, 2712
Eam1104I CTCTTC 1 cut(s) 695
EarI CTCTTC 1 cut(s) 695
EciI GGCGGA 2 cut(s) 12, 109
Ecl136II GAGCTC 2 cut(s) 1077, 2709
Eco105I TACGTA 1 cut(s) 939
Eco130I CCWWGG 6 cut(s) 71, 523, 1227, 1595, 2364, 2715
Eco24I GRGCYC 3 cut(s) 91, 1079, 2711
Eco31I GGTCTC 2 cut(s) 711, 2230
Eco32I GATATC 1 cut(s) 2057
Eco47I GGWCC 5 cut(s) 133, 339, 371, 1531, 2165
Eco47III AGCGCT 1 cut(s) 1912
Eco53kI GAGCTC 2 cut(s) 1077, 2709
Eco57I CTGAAG 3 cut(s) 281, 2271, 2429
Eco72I CACGTG 1 cut(s) 1721
EcoICRI GAGCTC 2 cut(s) 1077, 2709
EcoO109I RGGNCCY 5 cut(s) 87, 133, 258, 805, 1531
EcoRI GAATTC 1 cut(s) 560
EcoRII CCWGG 4 cut(s) 114, 135, 908, 2059
EcoRV GATATC 1 cut(s) 2057
EcoT14I CCWWGG 6 cut(s) 71, 523, 1227, 1595, 2364, 2715
EcoT22I ATGCAT 2 cut(s) 1158, 1518
EcoT38I GRGCYC 3 cut(s) 91, 1079, 2711
ErhI CCWWGG 6 cut(s) 71, 523, 1227, 1595, 2364, 2715
FalI AAGNNNNNCTT 2 cut(s) 1917, 1949
FaqI GGGAC 6 cut(s) 907, 1020, 1356, 1978, 2178, 2633
FauNDI CATATG 1 cut(s) 1286
FbaI TGATCA 3 cut(s) 250, 463, 1836
Fnu4HI GCNGC 8 cut(s) 347, 543, 980, 1426, 1725, 2325, 2445, 2739
FokI GGATG 8 cut(s) 755, 1112, 1303, 2359, 2732, 2738, 2825, 2963
FriOI GRGCYC 3 cut(s) 91, 1079, 2711
Fsp4HI GCNGC 8 cut(s) 347, 543, 980, 1426, 1725, 2325, 2445, 2739
FspBI CTAG 7 cut(s) 506, 1194, 1596, 1634, 1856, 2339, 2940
GlaI GCGC 3 cut(s) 241, 754, 1912
GluI GCNGC 8 cut(s) 347, 543, 980, 1426, 1725, 2325, 2445, 2739
GsuI CTGGAG 1 cut(s) 435
HaeII RGCGCY 1 cut(s) 1914
HapII CCGG 5 cut(s) 26, 77, 84, 91, 1349
HgaI GACGC 1 cut(s) 759
HhaI GCGC 3 cut(s) 242, 755, 1913
Hin1I GRCGYC 2 cut(s) 1144, 1272
Hin6I GCGC 3 cut(s) 240, 753, 1911
HinP1I GCGC 3 cut(s) 240, 753, 1911
HincII GTYRAC 2 cut(s) 1270, 1276
HindII GTYRAC 2 cut(s) 1270, 1276
HindIII AAGCTT 1 cut(s) 1602
HpaII CCGG 5 cut(s) 26, 77, 84, 91, 1349
HphI GGTGA 5 cut(s) 369, 491, 1038, 1814, 2222
Hpy166II GTNNAC 7 cut(s) 125, 1180, 1270, 1276, 1649, 2050, 2066
Hpy8I GTNNAC 7 cut(s) 125, 1180, 1270, 1276, 1649, 2050, 2066
HpyCH4III ACNGT 7 cut(s) 366, 726, 1280, 1422, 1509, 2691, 2798
HpyCH4IV ACGT 8 cut(s) 121, 938, 1144, 1272, 1720, 2170, 2391, 2680
HpyF10VI GCNNNNNNNGC 8 cut(s) 73, 239, 323, 539, 1733, 2141, 2441, 2918
HpyF3I CTNAG 8 cut(s) 57, 492, 1215, 1536, 1791, 1919, 2238, 2606
HpySE526I ACGT 8 cut(s) 121, 938, 1144, 1272, 1720, 2170, 2391, 2680
Hsp92I GRCGYC 2 cut(s) 1144, 1272
HspAI GCGC 3 cut(s) 240, 753, 1911
Kpn2I TCCGGA 1 cut(s) 1348
Ksp22I TGATCA 3 cut(s) 250, 463, 1836
LmnI GCTCC 5 cut(s) 45, 311, 454, 1594, 1826
Lsp1109I GCAGC 6 cut(s) 358, 554, 1736, 2336, 2456, 2725
LweI GCATC 4 cut(s) 1072, 1684, 2122, 2431
MaeI CTAG 7 cut(s) 506, 1194, 1596, 1634, 1856, 2339, 2940
MaeII ACGT 8 cut(s) 121, 938, 1144, 1272, 1720, 2170, 2391, 2680
MaeIII GTNAC 8 cut(s) 274, 440, 487, 775, 791, 1242, 1884, 2490
MfeI CAATTG 1 cut(s) 1785
MflI RGATCY 4 cut(s) 502, 1351, 1387, 2254
MhlI GDGCHC 6 cut(s) 91, 127, 1079, 1591, 2711, 2796
MlsI TGGCCA 1 cut(s) 2666
MluNI TGGCCA 1 cut(s) 2666
MlyI GAGTC 4 cut(s) 74, 680, 1836, 1940
MmeI TCCRAC 2 cut(s) 446, 2000
Mox20I TGGCCA 1 cut(s) 2666
Mph1103I ATGCAT 2 cut(s) 1158, 1518
MroI TCCGGA 1 cut(s) 1348
MscI TGGCCA 1 cut(s) 2666
MslI CAYNNNNRTG 3 cut(s) 762, 1289, 2102
Msp20I TGGCCA 1 cut(s) 2666
MspA1I CMGCKG 2 cut(s) 433, 1970
MspCI CTTAAG 1 cut(s) 2015
MspI CCGG 5 cut(s) 26, 77, 84, 91, 1349
MspR9I CCNGG 6 cut(s) 85, 91, 116, 137, 910, 2061
MunI CAATTG 1 cut(s) 1785
Mva1269I GAATGC 1 cut(s) 326
MvaI CCWGG 4 cut(s) 116, 137, 910, 2061
MvnI CGCG 2 cut(s) 240, 753
MwoI GCNNNNNNNGC 8 cut(s) 73, 239, 323, 539, 1733, 2141, 2441, 2918
NciI CCSGG 2 cut(s) 85, 91
NcoI CCATGG 3 cut(s) 71, 523, 2715
NdeI CATATG 1 cut(s) 1286
NheI GCTAGC 1 cut(s) 2338
NlaIV GGNNCC 6 cut(s) 88, 89, 907, 1353, 1533, 2166
NmeAIII GCCGAG 2 cut(s) 153, 2690
NmuCI GTSAC 3 cut(s) 1242, 1884, 2490
NsiI ATGCAT 2 cut(s) 1158, 1518
NspI RCATGY 1 cut(s) 1160
OliI CACNNNNGTG 1 cut(s) 2102
PagI TCATGA 2 cut(s) 1132, 1435
PctI GAATGC 1 cut(s) 326
PfeI GAWTC 7 cut(s) 48, 212, 1294, 1501, 1862, 1984, 2117
PflFI GACNNNGTC 1 cut(s) 1184
PflMI CCANNNNNTGG 1 cut(s) 681
PfoI TCCNGGA 1 cut(s) 908
PkrI GCNGC 8 cut(s) 348, 544, 981, 1427, 1726, 2326, 2446, 2740
PleI GAGTC 4 cut(s) 74, 679, 1836, 1940
PmaCI CACGTG 1 cut(s) 1721
PmlI CACGTG 1 cut(s) 1721
PpsI GAGTC 4 cut(s) 74, 679, 1836, 1940
Ppu21I YACGTR 3 cut(s) 939, 1721, 2171
PpuMI RGGWCCY 2 cut(s) 133, 1531
PshAI GACNNNNGTC 2 cut(s) 233, 2029
PshBI ATTAAT 2 cut(s) 357, 2640
PsiI TTATAA 2 cut(s) 1452, 1622
Psp124BI GAGCTC 2 cut(s) 1079, 2711
Psp1406I AACGTT 1 cut(s) 2680
Psp5II RGGWCCY 2 cut(s) 133, 1531
Psp6I CCWGG 4 cut(s) 114, 135, 908, 2059
PspCI CACGTG 1 cut(s) 1721
PspGI CCWGG 4 cut(s) 114, 135, 908, 2059
PspN4I GGNNCC 6 cut(s) 88, 89, 907, 1353, 1533, 2166
PspOMI GGGCCC 1 cut(s) 87
PspPPI RGGWCCY 2 cut(s) 133, 1531
PstI CTGCAG 1 cut(s) 1500
PsuI RGATCY 4 cut(s) 502, 1351, 1387, 2254
PsyI GACNNNGTC 1 cut(s) 1184
PvuII CAGCTG 1 cut(s) 1970
RsaI GTAC 7 cut(s) 55, 302, 553, 1650, 2043, 2051, 2693
RsaNI GTAC 7 cut(s) 54, 301, 552, 1649, 2042, 2050, 2692
RseI CAYNNNNRTG 3 cut(s) 762, 1289, 2102
Rsr2I CGGWCCG 1 cut(s) 339
RsrII CGGWCCG 1 cut(s) 339
SacI GAGCTC 2 cut(s) 1079, 2711
SatI GCNGC 8 cut(s) 347, 543, 980, 1426, 1725, 2325, 2445, 2739
SchI GAGTC 4 cut(s) 74, 680, 1836, 1940
ScrFI CCNGG 6 cut(s) 85, 91, 116, 137, 910, 2061
SduI GDGCHC 6 cut(s) 91, 127, 1079, 1591, 2711, 2796
SfaNI GCATC 4 cut(s) 1072, 1684, 2122, 2431
SfcI CTRYAG 2 cut(s) 839, 1496
SgrAI CRCCGGYG 1 cut(s) 25
SinI GGWCC 5 cut(s) 133, 339, 371, 1531, 2165
SmiMI CAYNNNNRTG 3 cut(s) 762, 1289, 2102
SmlI CTYRAG 5 cut(s) 583, 1364, 1398, 2015, 2227
SmoI CTYRAG 5 cut(s) 583, 1364, 1398, 2015, 2227
SnaBI TACGTA 1 cut(s) 939
SspMI CTAG 7 cut(s) 506, 1194, 1596, 1634, 1856, 2339, 2940
SstI GAGCTC 2 cut(s) 1079, 2711
StyD4I CCNGG 6 cut(s) 83, 89, 114, 135, 908, 2059
StyI CCWWGG 6 cut(s) 71, 523, 1227, 1595, 2364, 2715
TaaI ACNGT 7 cut(s) 366, 726, 1280, 1422, 1509, 2691, 2798
TaiI ACGT 8 cut(s) 124, 941, 1147, 1275, 1723, 2173, 2394, 2683
TaqI TCGA 4 cut(s) 227, 1504, 1702, 2549
TaqII GACCGA 2 cut(s) 356, 735
TatI WGTACW 2 cut(s) 53, 300
TauI GCSGC 2 cut(s) 982, 1428
TfiI GAWTC 7 cut(s) 48, 212, 1294, 1501, 1862, 1984, 2117
TscAI CASTG 6 cut(s) 337, 459, 903, 1427, 1500, 2254
TseFI GTSAC 3 cut(s) 1242, 1884, 2490
TseI GCWGC 6 cut(s) 346, 542, 1724, 2324, 2444, 2738
Tsp45I GTSAC 3 cut(s) 1242, 1884, 2490
TspGWI ACGGA 3 cut(s) 32, 1470, 2037
TspRI CASTG 6 cut(s) 337, 459, 903, 1427, 1500, 2254
Tth111I GACNNNGTC 1 cut(s) 1184
Van91I CCANNNNNTGG 1 cut(s) 681
Vha464I CTTAAG 1 cut(s) 2015
VneI GTGCAC 1 cut(s) 123
VpaK11BI GGWCC 5 cut(s) 133, 339, 371, 1531, 2165
VspI ATTAAT 2 cut(s) 357, 2640
XapI RAATTY 3 cut(s) 560, 730, 2562
XbaI TCTAGA 2 cut(s) 505, 1855
XceI RCATGY 1 cut(s) 1160
XcmI CCANNNNNNNNNTGG 2 cut(s) 1564, 1765
XmaJI CCTAGG 1 cut(s) 1595
XspI CTAG 7 cut(s) 506, 1194, 1596, 1634, 1856, 2339, 2940
ZraI GACGTC 2 cut(s) 1145, 1273
Zsp2I ATGCAT 2 cut(s) 1158, 1518
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.