pycom02g05070
ERF Family

Probably involved in the RNA silencing pathway and required for the generation of small interfering RNAs (siRNAs)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0000505
Physical Location & Seq
Forward (+)
3332760 .. 3337648
4889 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g05070.2

Sequence Viewer

Length: 2172 bp
ATGGTGAAATTTGCCGATGCGAAAGATCCCTGGAAGCAGAGGGACCTTTATGCTGACTATAGCAAGGTCGCTAGAGAAGGGATTCTTGTTGGTTTGCGCCGATATCGTTTCTTTGTGTTCAAAGATGGTGGCAATAAAGAAAAGACAAAAAGCTCGACTTCATCACCTGTGAAGTGTTACTTTATCCGTGTGGACTCTAGTGCCGCGATTGACAGGGGAGAAGATTATAAAATATCTAATAAAACGATTCACGAAGCAAGATGCAATTTCATGCATCCACATACTGTGTCCACTGTGAGCAACTACATGATTAGGTTTTCACTTATTTTGTCTAAGACAGAGAGCTTAGAAGTAAAAGATTGGTCTCTTGTGAGGGTTGACGACATTGATGATGAGTATTGTCTGGATGCATACGGTAATCATATCTATACAGACGGCAAGCCTCTTATACATTCAGATGGAACTGGATTCATATCAGAGGATTTGGCGTCACTTTGTCCGAAAGAATTACTGAAAAAAGAGTTCAGCAATGAATACTTTGAGCCTTTGCTCATGCAGTCCCGACTTTTCTACAAGGGCCGTGCTGTGAAGGGAACCTTTCTAATCAATAAAAAGCTACCACAAAAAACGATCCAGATTCGACCATCAATGATTAAAGTTGAGACAGATCGAAAGATGCTGGATGGTCAAACAGTGAATTCGTTGGAGATAGTTGGAGTAAGTACAAAGCCTAGGAATACAAAATTTTCGAGGAGTTTAATTTCCCTTCTATGCCATGGAGGGGTGCCGAAAGATTATTTCATGGAGTTACTGATGAAAGATCTGGAAAATACTCATGGTGTTTTCTGCAATAGGCGTGCAGCAGCCAAAATTGCCTTTAGCTACGGCGGGATGGATGATGACTACAACACGTTTAAGATGATCTCTTGTGATATTCCTCTAGAAGAATCATATTTGCAACATCGTTTGTCATTCCTGATGAAAGAGGAAAATACCAGTCTTAAAAAAGGGAAAATTTCTAGTCCTCAGAGTTACATGTTGATGGGGACTACTGATCCAACAGGGATTCTGGAAAAGGATCAAGTTTGCGTTCTTCTTGACAGCGGGCCAATTCAAGGAGAGGTCCTGGTGTACCGGCATCCAGGTTTACATTTTGGTGATGTTCATGTTTTGAAGGCCACCTACGTGGAGGAGTTGAAAGAATTTGTCGGAAATAGTGGCTTTGCTATATTCTTCTCTCGCAAAGGTTCGCGTTCAGTAGCTGATGAAATGGGTGGTGGAGATTTTGATGGCGATCTATATTGGGTCTCAAGAAACCCACGGCTTTTAGAATGTTTCAAACAAAGTGAACCTTGGATCGATACTTCATCAACGCCCAGAGCTGCCACCACCTTACCACCAAGTGCGCTTTTGCCCGATCATATAGAGGATGTGCTCTTTAAACAATTCTTGACAACTAGGTTTGAACCGAGTTATGCGATGAGTACGGCTTCTGACAACTGGTTGGCTTATATGGATGAATATTTGACCCTGGGAGATGGTCATGAGAAAACCCTTACGAAGGCAAAAATGCTGCGGTTGATTGATTTATACTACGATGCTTTGGATGCTCCAAAAAAATGTCGAACGGTCAAAATCCCCAATGATTTGAAACCGCGGTCATTCCCCCATTACATGGGTAAGCTCAACTCGTACAAATCAACTTCCATTTTGGGATCAATTTATGACGCGTTGGAGGAATATCAACCGGAAGACACTTCTGACGAAGTAGTGGAAAAACTTCCTTATTTCGATGTTGAAGACCTCCCCGAGCAGTGTCTGAAGAAGTGGTACGAACTCTACGAGCAGTACAGGAGTGAAATGACCTCCGTCTTGCAATACGATGATAAAGAGGGCAAGAATAAGGCTGCCGATAACCTCATAAGACGGTATAAAGAGATTCTGTATGGTTGTGAGGACTTAGAGAAGAGCACAAGGCCATTGGATGAAATATTCAACGAGGCACTCGCCATATATCGTTTCGCGTATGACTACGCTTGGAAAGCAAACGATGTAAGGAAATGTGGGTTTGCATGGAAAGTTGCAGGTTCAGCTCTCTGTAAGTATTACAAGACCAAACACGAGGACCGCACGTTTGAAGTCTCGTTCTCTGTTCTCAAGGACATCCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RdRP

Protein Analysis

724

Amino Acids

83.05

Weight (kDa)

6.18

Isoelectric Point (pI)

41.81

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000505)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G19910 AT2G19910 AT2G19920 AT2G19920 AT2G19920 AT2G19920 AT2G19930 AT2G19930 AT2G19930
fragaria_vesca FvH4_1g05980 FvH4_1g05990 FvH4_1g05990 FvH4_1g09718
malus_domestica MD02G1064400.v1.1 MD15G1195300.v1.1 MD15G1195400.v1.1
prunus_persica Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1
pyrus_communis pycom02g05070 pycom15g17460
rosa_chinensis RchiOBHm_Chr2g0091671 RchiOBHm_Chr2g0091681 RchiOBHm_Chr2g0091691 RchiOBHm_Chr2g0091701 RchiOBHm_Chr2g0091711 RchiOBHm_Chr2g0091721 RchiOBHm_Chr2g0091731 RchiOBHm_Chr5g0014401
rosa_laevigata RLG00000013772 RLG00000016242 RLG00000016244 RLG00000016245 RLG00000016246 RLG00000032105
rosa_multiflora Rmu_co8374041.1_g000001 Rmu_co8478121.1_g000001 Rmu_sc0000897.1_g000005 Rmu_sc0000897.1_g000009
rosa_roxburghii Rroxscaffold_1G00062180 Rroxscaffold_1G00062190 Rroxscaffold_1G00068800 Rroxscaffold_2G00149790 Rroxscaffold_2G00149800 Rroxscaffold_2G00149810
rosa_rugosa Rorug02G0019600 Rorug02G0019700 Rorug02G0019800 Rorug02G0019900 Rorug02G0019900 Rorug02G0020000 Rorug02G0020000 Rorug02G0020100 Rorug02G0020100 Rorug02G0020200 Rorug05G0017800
rosa_samantha Rh2AG064700 Rh2AG064800 Rh2AG064900 Rh2BG063800 Rh2BG063900 Rh2BG064000 Rh2BG064100 Rh2CG065500 Rh2CG065600 Rh2CG065700 Rh2DG063900 Rh2DG064000 Rh2DG064100 Rh2DG064200 Rh2DG381700 Rh5AG056700 Rh5AG056800 Rh5AG056900 Rh5AG112600 Rh5BG109300 Rh5CG063800 Rh5CG121100 Rh5DG053100 Rh5DG108300 Rh6CG100100
rosa_wichuraiana Rw2G005560 Rw2G005580 Rw2G005590 Rw5G005370 Rw5G009770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 228
Acc36I ACCTGC 1 cut(s) 2075
AccB1I GGYRCC 1 cut(s) 784
AccB7I CCANNNNNTGG 1 cut(s) 1675
AccII CGCG 5 cut(s) 206, 1252, 1657, 1730, 2024
AciI CCGC 7 cut(s) 204, 888, 1104, 1576, 1655, 1657, 2128
AclWI GGATC 6 cut(s) 20, 625, 1049, 1086, 1364, 1723
AcsI RAATTY 5 cut(s) 8, 697, 743, 1014, 1202
AcuI CTGAAG 1 cut(s) 1841
AcyI GRCGYC 1 cut(s) 488
AdeI CACNNNGTG 1 cut(s) 1403
AfaI GTAC 6 cut(s) 724, 1133, 1486, 1694, 1832, 1850
AfiI CCNNNNNNNGG 4 cut(s) 781, 1115, 1561, 1675
AflIII ACRYGT 3 cut(s) 909, 1035, 1728
AjnI CCWGG 4 cut(s) 29, 1125, 1141, 1530
AleI CACNNNNGTG 1 cut(s) 1184
AluBI AGCT 8 cut(s) 153, 345, 616, 882, 1262, 1382, 1684, 2093
AluI AGCT 8 cut(s) 153, 345, 616, 882, 1262, 1382, 1684, 2093
Alw21I GWGCWC 2 cut(s) 1437, 1973
Alw26I GTCTC 4 cut(s) 369, 656, 1312, 2146
AlwI GGATC 6 cut(s) 20, 625, 1049, 1086, 1364, 1723
AlwNI CAGNNNCTG 2 cut(s) 1262, 1819
Ama87I CYCGRG 1 cut(s) 1808
AoxI GGCC 4 cut(s) 577, 1106, 1176, 1976
ApeKI GCWGC 5 cut(s) 860, 863, 1382, 1573, 1907
ApoI RAATTY 5 cut(s) 8, 697, 743, 1014, 1202
ArsI GACNNNNNNTTYG 2 cut(s) 2117, 2149
Asp700I GAANNNNTTC 2 cut(s) 81, 1779
AspA2I CCTAGG 1 cut(s) 731
AspLEI GCGC 2 cut(s) 99, 1408
AspS9I GGNCC 5 cut(s) 43, 577, 1106, 1123, 2125
AsuHPI GGTGA 3 cut(s) 16, 156, 1169
AvaI CYCGRG 1 cut(s) 1808
AvaII GGWCC 3 cut(s) 43, 1123, 2125
AvrII CCTAGG 1 cut(s) 731
BanI GGYRCC 1 cut(s) 784
BarI GAAGNNNNNNTAC 4 cut(s) 1166, 1198, 1814, 1846
BauI CACGAG 1 cut(s) 2120
BbsI GAAGAC 2 cut(s) 1758, 1806
Bbv12I GWGCWC 2 cut(s) 1437, 1973
BbvI GCAGC 5 cut(s) 872, 875, 1369, 1560, 1894
BccI CCATC 8 cut(s) 119, 452, 652, 677, 886, 1036, 1283, 1532
BceAI ACGGC 5 cut(s) 451, 564, 901, 1337, 1503
BciT130I CCWGG 4 cut(s) 31, 1127, 1143, 1532
BcoDI GTCTC 4 cut(s) 369, 656, 1312, 2146
BfaI CTAG 6 cut(s) 72, 198, 732, 941, 1020, 1458
BfmI CTRYAG 1 cut(s) 58
BfuAI ACCTGC 1 cut(s) 2075
BglII AGATCT 1 cut(s) 820
BisI GCNGC 6 cut(s) 204, 861, 864, 1383, 1574, 1908
BlnI CCTAGG 1 cut(s) 731
BlsI GCNGC 6 cut(s) 205, 862, 865, 1384, 1575, 1909
Bme1390I CCNGG 4 cut(s) 31, 1127, 1143, 1532
Bme18I GGWCC 3 cut(s) 43, 1123, 2125
BmeT110I CYCGRG 1 cut(s) 1808
BmgT120I GGNCC 5 cut(s) 43, 577, 1106, 1123, 2125
BmiI GGNNCC 3 cut(s) 44, 595, 786
BmrFI CCNGG 4 cut(s) 31, 1127, 1143, 1532
BmsI GCATC 8 cut(s) 7, 251, 283, 397, 666, 1147, 1588, 1597
BoxI GACNNNNGTC 1 cut(s) 1868
BpiI GAAGAC 2 cut(s) 1758, 1806
BpuEI CTTGAG 2 cut(s) 1294, 2141
Bsa29I ATCGAT 1 cut(s) 1359
BsaAI YACGTR 1 cut(s) 1186
BsaBI GATNNNNATC 2 cut(s) 472, 1293
BsaHI GRCGYC 1 cut(s) 488
BsaI GGTCTC 2 cut(s) 369, 1312
BsaJI CCNNGG 8 cut(s) 29, 731, 775, 1319, 1352, 1530, 1531, 1655
BsaWI WCCGGW 1 cut(s) 1747
Bsc4I CCNNNNNNNGG 4 cut(s) 781, 1115, 1561, 1675
Bse118I RCCGGY 1 cut(s) 1134
Bse1I ACTGG 3 cut(s) 469, 996, 1505
Bse3DI GCAATG 1 cut(s) 535
Bse8I GATNNNNATC 2 cut(s) 472, 1293
BseBI CCWGG 4 cut(s) 31, 1127, 1143, 1532
BseCI ATCGAT 1 cut(s) 1359
BseDI CCNNGG 8 cut(s) 29, 731, 775, 1319, 1352, 1530, 1531, 1655
BseJI GATNNNNATC 2 cut(s) 472, 1293
BseLI CCNNNNNNNGG 4 cut(s) 781, 1115, 1561, 1675
BseMI GCAATG 1 cut(s) 535
BseMII CTCAG 1 cut(s) 1040
BseNI ACTGG 3 cut(s) 469, 996, 1505
BseRI GAGGAG 2 cut(s) 766, 1205
BseXI GCAGC 5 cut(s) 872, 875, 1369, 1560, 1894
BsgI GTGCAG 1 cut(s) 879
Bsh1236I CGCG 5 cut(s) 206, 1252, 1657, 1730, 2024
BshFI GGCC 4 cut(s) 579, 1108, 1178, 1978
BshNI GGYRCC 1 cut(s) 784
BshVI ATCGAT 1 cut(s) 1359
BsiHKAI GWGCWC 2 cut(s) 1437, 1973
BsiHKCI CYCGRG 1 cut(s) 1808
BsiSI CCGG 2 cut(s) 1135, 1748
BslFI GGGAC 3 cut(s) 56, 544, 1060
BslI CCNNNNNNNGG 4 cut(s) 781, 1115, 1561, 1675
BsmAI GTCTC 4 cut(s) 369, 656, 1312, 2146
BsmFI GGGAC 3 cut(s) 56, 544, 1060
BsnI GGCC 4 cut(s) 579, 1108, 1178, 1978
Bso31I GGTCTC 2 cut(s) 369, 1312
BsoBI CYCGRG 1 cut(s) 1808
Bsp1286I GDGCHC 2 cut(s) 1437, 1973
Bsp19I CCATGG 1 cut(s) 775
BspACI CCGC 7 cut(s) 204, 888, 1104, 1576, 1655, 1657, 2128
BspANI GGCC 4 cut(s) 579, 1108, 1178, 1978
BspCNI CTCAG 1 cut(s) 1039
BspDI ATCGAT 1 cut(s) 1359
BspFNI CGCG 5 cut(s) 206, 1252, 1657, 1730, 2024
BspHI TCATGA 1 cut(s) 1543
BspLI GGNNCC 3 cut(s) 44, 595, 786
BspMI ACCTGC 1 cut(s) 2075
BspPI GGATC 6 cut(s) 20, 625, 1049, 1086, 1364, 1723
BspQI GCTCTTC 1 cut(s) 1961
BspT107I GGYRCC 1 cut(s) 784
BspTNI GGTCTC 2 cut(s) 369, 1312
BsrDI GCAATG 1 cut(s) 535
BsrFI RCCGGY 1 cut(s) 1134
BsrI ACTGG 3 cut(s) 469, 996, 1505
BssAI RCCGGY 1 cut(s) 1134
BssECI CCNNGG 8 cut(s) 29, 731, 775, 1319, 1352, 1530, 1531, 1655
BssNI GRCGYC 1 cut(s) 488
BssSI CACGAG 1 cut(s) 2120
BssT1I CCWWGG 3 cut(s) 731, 775, 1352
Bst2BI CACGAG 1 cut(s) 2120
Bst2UI CCWGG 4 cut(s) 31, 1127, 1143, 1532
Bst4CI ACNGT 6 cut(s) 286, 295, 416, 694, 1630, 1929
Bst6I CTCTTC 1 cut(s) 1961
BstACI GRCGYC 1 cut(s) 488
BstBAI YACGTR 1 cut(s) 1186
BstC8I GCNNGC 3 cut(s) 440, 858, 1106
BstDEI CTNAG 4 cut(s) 333, 346, 1026, 1960
BstDSI CCRYGG 3 cut(s) 775, 1319, 1655
BstENI CCTNNNNNAGG 1 cut(s) 1559
BstFNI CGCG 5 cut(s) 206, 1252, 1657, 1730, 2024
BstHHI GCGC 2 cut(s) 99, 1408
BstMAI GTCTC 4 cut(s) 369, 656, 1312, 2146
BstMWI GCNNNNNNNGC 4 cut(s) 872, 1607, 2042, 2090
BstNI CCWGG 4 cut(s) 31, 1127, 1143, 1532
BstNSI RCATGY 1 cut(s) 1039
BstPAI GACNNNNGTC 1 cut(s) 1868
BstSCI CCNGG 4 cut(s) 29, 1125, 1141, 1530
BstSFI CTRYAG 1 cut(s) 58
BstUI CGCG 5 cut(s) 206, 1252, 1657, 1730, 2024
BstV1I GCAGC 5 cut(s) 872, 875, 1369, 1560, 1894
BstV2I GAAGAC 2 cut(s) 1758, 1806
BstX2I RGATCY 2 cut(s) 25, 820
BstXI CCANNNNNNTGG 1 cut(s) 1186
BstYI RGATCY 2 cut(s) 25, 820
Bsu15I ATCGAT 1 cut(s) 1359
BsuRI GGCC 4 cut(s) 579, 1108, 1178, 1978
BsuTUI ATCGAT 1 cut(s) 1359
BtgI CCRYGG 3 cut(s) 775, 1319, 1655
BtgZI GCGATG 1 cut(s) 1493
BtsI GCAGTG 1 cut(s) 1820
BtsIMutI CAGTG 3 cut(s) 291, 699, 1820
BveI ACCTGC 1 cut(s) 2075
Cac8I GCNNGC 3 cut(s) 440, 858, 1106
CaiI CAGNNNCTG 2 cut(s) 1262, 1819
CciI TCATGA 1 cut(s) 1543
CfoI GCGC 2 cut(s) 99, 1408
Cfr10I RCCGGY 1 cut(s) 1134
Cfr13I GGNCC 5 cut(s) 43, 577, 1106, 1123, 2125
Cfr42I CCGCGG 1 cut(s) 1658
ClaI ATCGAT 1 cut(s) 1359
CseI GACGC 2 cut(s) 477, 1736
Csp6I GTAC 6 cut(s) 723, 1132, 1485, 1693, 1831, 1849
CspCI CAANNNNNGTGG 2 cut(s) 109, 144
CviQI GTAC 6 cut(s) 723, 1132, 1485, 1693, 1831, 1849
DdeI CTNAG 4 cut(s) 333, 346, 1026, 1960
DraI TTTAAA 1 cut(s) 1441
DraIII CACNNNGTG 1 cut(s) 1403
Eam1104I CTCTTC 1 cut(s) 1961
EarI CTCTTC 1 cut(s) 1961
Eco130I CCWWGG 3 cut(s) 731, 775, 1352
Eco31I GGTCTC 2 cut(s) 369, 1312
Eco32I GATATC 1 cut(s) 104
Eco47I GGWCC 3 cut(s) 43, 1123, 2125
Eco57I CTGAAG 1 cut(s) 1841
Eco88I CYCGRG 1 cut(s) 1808
EcoNI CCTNNNNNAGG 1 cut(s) 1559
EcoO109I RGGNCCY 2 cut(s) 43, 1123
EcoRI GAATTC 1 cut(s) 697
EcoRII CCWGG 4 cut(s) 29, 1125, 1141, 1530
EcoRV GATATC 1 cut(s) 104
EcoT14I CCWWGG 3 cut(s) 731, 775, 1352
EcoT22I ATGCAT 2 cut(s) 276, 412
ErhI CCWWGG 3 cut(s) 731, 775, 1352
FaqI GGGAC 3 cut(s) 56, 544, 1060
FauI CCCGC 2 cut(s) 881, 1097
Fnu4HI GCNGC 6 cut(s) 204, 861, 864, 1383, 1574, 1908
Fsp4HI GCNGC 6 cut(s) 204, 861, 864, 1383, 1574, 1908
FspBI CTAG 6 cut(s) 72, 198, 732, 941, 1020, 1458
GlaI GCGC 2 cut(s) 98, 1407
GluI GCNGC 6 cut(s) 204, 861, 864, 1383, 1574, 1908
HaeIII GGCC 4 cut(s) 579, 1108, 1178, 1978
HapII CCGG 2 cut(s) 1135, 1748
HgaI GACGC 2 cut(s) 477, 1736
HhaI GCGC 2 cut(s) 99, 1408
Hin1I GRCGYC 1 cut(s) 488
Hin6I GCGC 2 cut(s) 97, 1406
HinP1I GCGC 2 cut(s) 97, 1406
HincII GTYRAC 1 cut(s) 379
HindII GTYRAC 1 cut(s) 379
HinfI GANTC 8 cut(s) 82, 194, 247, 468, 637, 947, 1066, 1939
HpaII CCGG 2 cut(s) 1135, 1748
HphI GGTGA 3 cut(s) 16, 156, 1169
Hpy166II GTNNAC 6 cut(s) 193, 291, 379, 1132, 1148, 1349
Hpy188I TCNGA 8 cut(s) 457, 478, 501, 1029, 1211, 1495, 1762, 1821
Hpy8I GTNNAC 6 cut(s) 193, 291, 379, 1132, 1148, 1349
HpyAV CCTTC 5 cut(s) 71, 583, 776, 1168, 1555
HpyCH4III ACNGT 6 cut(s) 286, 295, 416, 694, 1630, 1929
HpyCH4IV ACGT 3 cut(s) 911, 1185, 2132
HpyF10VI GCNNNNNNNGC 4 cut(s) 872, 1607, 2042, 2090
HpyF3I CTNAG 4 cut(s) 333, 346, 1026, 1960
HpySE526I ACGT 3 cut(s) 911, 1185, 2132
Hsp92I GRCGYC 1 cut(s) 488
HspAI GCGC 2 cut(s) 97, 1406
KspI CCGCGG 1 cut(s) 1658
LguI GCTCTTC 1 cut(s) 1961
LmnI GCTCC 1 cut(s) 1615
Lsp1109I GCAGC 5 cut(s) 872, 875, 1369, 1560, 1894
LweI GCATC 8 cut(s) 7, 251, 283, 397, 666, 1147, 1588, 1597
MaeI CTAG 6 cut(s) 72, 198, 732, 941, 1020, 1458
MaeII ACGT 3 cut(s) 911, 1185, 2132
MaeIII GTNAC 4 cut(s) 176, 489, 807, 1031
MboII GAAGA 8 cut(s) 233, 956, 1085, 1225, 1763, 1811, 1834, 1978
MflI RGATCY 2 cut(s) 25, 820
MhlI GDGCHC 2 cut(s) 1437, 1973
MluI ACGCGT 1 cut(s) 1728
MlyI GAGTC 1 cut(s) 188
MmeI TCCRAC 5 cut(s) 684, 694, 1082, 1189, 1713
Mph1103I ATGCAT 2 cut(s) 276, 412
MroXI GAANNNNTTC 2 cut(s) 81, 1779
MseI TTAA 5 cut(s) 654, 758, 915, 1002, 1440
MslI CAYNNNNRTG 4 cut(s) 456, 1040, 1155, 1184
MspA1I CMGCKG 2 cut(s) 1104, 1657
MspI CCGG 2 cut(s) 1135, 1748
MspR9I CCNGG 4 cut(s) 31, 1127, 1143, 1532
MvaI CCWGG 4 cut(s) 31, 1127, 1143, 1532
MvnI CGCG 5 cut(s) 206, 1252, 1657, 1730, 2024
MwoI GCNNNNNNNGC 4 cut(s) 872, 1607, 2042, 2090
NcoI CCATGG 1 cut(s) 775
NlaIV GGNNCC 3 cut(s) 44, 595, 786
NmuCI GTSAC 1 cut(s) 489
NsiI ATGCAT 2 cut(s) 276, 412
NspI RCATGY 1 cut(s) 1039
OliI CACNNNNGTG 1 cut(s) 1184
PagI TCATGA 1 cut(s) 1543
PasI CCCWGGG 1 cut(s) 1531
PciI ACATGT 1 cut(s) 1035
PciSI GCTCTTC 1 cut(s) 1961
PcsI WCGNNNNNNNCGW 1 cut(s) 1839
PdmI GAANNNNTTC 2 cut(s) 81, 1779
PfeI GAWTC 7 cut(s) 82, 247, 468, 637, 947, 1066, 1939
PflMI CCANNNNNTGG 1 cut(s) 1675
PkrI GCNGC 6 cut(s) 205, 862, 865, 1384, 1575, 1909
PleI GAGTC 1 cut(s) 188
PpsI GAGTC 1 cut(s) 188
Ppu21I YACGTR 1 cut(s) 1186
PpuMI RGGWCCY 2 cut(s) 43, 1123
PscI ACATGT 1 cut(s) 1035
PshAI GACNNNNGTC 1 cut(s) 1868
PsiI TTATAA 1 cut(s) 228
Psp5II RGGWCCY 2 cut(s) 43, 1123
Psp6I CCWGG 4 cut(s) 29, 1125, 1141, 1530
PspGI CCWGG 4 cut(s) 29, 1125, 1141, 1530
PspN4I GGNNCC 3 cut(s) 44, 595, 786
PspPI GGNCC 5 cut(s) 43, 577, 1106, 1123, 2125
PspPPI RGGWCCY 2 cut(s) 43, 1123
PstNI CAGNNNCTG 2 cut(s) 1262, 1819
PsuI RGATCY 2 cut(s) 25, 820
RsaI GTAC 6 cut(s) 724, 1133, 1486, 1694, 1832, 1850
RsaNI GTAC 6 cut(s) 723, 1132, 1485, 1693, 1831, 1849
RseI CAYNNNNRTG 4 cut(s) 456, 1040, 1155, 1184
SacII CCGCGG 1 cut(s) 1658
SapI GCTCTTC 1 cut(s) 1961
SaqAI TTAA 5 cut(s) 654, 758, 915, 1002, 1440
SatI GCNGC 6 cut(s) 204, 861, 864, 1383, 1574, 1908
Sau96I GGNCC 5 cut(s) 43, 577, 1106, 1123, 2125
SchI GAGTC 1 cut(s) 188
ScrFI CCNGG 4 cut(s) 31, 1127, 1143, 1532
SduI GDGCHC 2 cut(s) 1437, 1973
SfaNI GCATC 8 cut(s) 7, 251, 283, 397, 666, 1147, 1588, 1597
SfcI CTRYAG 1 cut(s) 58
Sfr303I CCGCGG 1 cut(s) 1658
SgrBI CCGCGG 1 cut(s) 1658
SinI GGWCC 3 cut(s) 43, 1123, 2125
SmiMI CAYNNNNRTG 4 cut(s) 456, 1040, 1155, 1184
SmlI CTYRAG 2 cut(s) 1309, 2156
SmoI CTYRAG 2 cut(s) 1309, 2156
SsiI CCGC 7 cut(s) 204, 888, 1104, 1576, 1655, 1657, 2128
SspI AATATT 2 cut(s) 1523, 1992
SspMI CTAG 6 cut(s) 72, 198, 732, 941, 1020, 1458
StyD4I CCNGG 4 cut(s) 29, 1125, 1141, 1530
StyI CCWWGG 3 cut(s) 731, 775, 1352
TaaI ACNGT 6 cut(s) 286, 295, 416, 694, 1630, 1929
TaiI ACGT 3 cut(s) 914, 1188, 2135
TaqI TCGA 7 cut(s) 155, 640, 670, 749, 1359, 1624, 1791
TatI WGTACW 2 cut(s) 722, 1848
TauI GCSGC 1 cut(s) 206
TfiI GAWTC 7 cut(s) 82, 247, 468, 637, 947, 1066, 1939
Tru1I TTAA 5 cut(s) 654, 758, 915, 1002, 1440
Tru9I TTAA 5 cut(s) 654, 758, 915, 1002, 1440
TscAI CASTG 3 cut(s) 298, 699, 1820
TseFI GTSAC 1 cut(s) 489
TseI GCWGC 5 cut(s) 860, 863, 1382, 1573, 1907
Tsp45I GTSAC 1 cut(s) 489
TspGWI ACGGA 2 cut(s) 176, 1858
TspRI CASTG 3 cut(s) 298, 699, 1820
Van91I CCANNNNNTGG 1 cut(s) 1675
VpaK11BI GGWCC 3 cut(s) 43, 1123, 2125
XagI CCTNNNNNAGG 1 cut(s) 1559
XapI RAATTY 5 cut(s) 8, 697, 743, 1014, 1202
XbaI TCTAGA 1 cut(s) 940
XceI RCATGY 1 cut(s) 1039
XmaJI CCTAGG 1 cut(s) 731
XmnI GAANNNNTTC 2 cut(s) 81, 1779
XspI CTAG 6 cut(s) 72, 198, 732, 941, 1020, 1458
Zsp2I ATGCAT 2 cut(s) 276, 412
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.