Rh5AG112600
ERF Family

Probably involved in the RNA silencing pathway and required for the generation of small interfering RNAs (siRNAs)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0000505
Physical Location & Seq
Reverse (-)
10371477 .. 10381620
10144 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG112600.1

Sequence Viewer

Length: 2565 bp
ATGTTCGGCGTGCAGCTACTCGACTCCGTGCAGCAATCGCTGGATCGAATATGTCACCTTAAGAAGCAACCGCAGCCGGACGACGGCATCAGAAGAAAGCTGGCATTGCTGAGTGAGCAAGACGCCCTCTACTATTCTGATTGGGATTCTCAAAGAACACACCACTACTATTGTTATGTTTCCGTGGATGGGAGTTGCAAGTTCAAGGGTCCCTTCCTAGACAAACCAAGAACTCTCCTACAGAAATGGTTAGAAGATGACAATGTTTTAGAGGTGAAATTTACCCAAGGGGAAGGTTCCAGTATTCTTTTTGATAATAATTATGGTACATATAGCAAGTTAATGCGAGAAGGCATTCTTGTCGGTTTGCGGCGATATGGTCACTTTGTGCACAGTGTTGGTGGTAACAAAGGAAAGAATAAGTTGAAAGACCCAACTTCATCACCTGTGAAATGTTACTTTGTTCGTATGAGCTCTGATGCAGCGATAGACATGGGTAAATTTTATAAGCTAGCTGGCAAAACGATGCATGAAGGAAGATCTCTTTTGACGCACGTACACACTGGGCCTAGTTTGGCTAATTACATGGCTAGGTTATCTCTCATTTTGTCCAAGACAGTTACTTTAGAAGTGAATTGGTCACTTGTCAAGGTTGAGTTCATCAATGAAGAATACTGTCTCGATGAGTCTGGTAATCCCATCTATGTAGATGGAAAAGCTTGTATACATACTGATGGCACGGGATTTATATCTGCGGATCTGGCTTTACTTTGTCCTGAGATAGCACACAAAGGAGAGCTTATCAATAATGAACACATCGAGGGACTTGATGATCCTGATGATGAACTTGAGGACAACATTCTGGAATGGAAACAGCCAGGCACTAGAACTCAGGAACTGCCTTTGCTCATGCAGTTCCGACTTTTTTACAAAGGCTATGCTATCAAAGGAACTTTTCTTGTCAATAAACTGCTTCCACCAAACACATTGCAGATTCGACCGTCAATGGTCAAAGTTGAGCCAGATCCAAAACTGTCAGATCCTCAAACAGTGAATTCACTGGAGATAGTAGGAACAAGTAAACTTACTAAGAGAACAACTTTGTCGAGGAACTTGATTGCACTTTTATACCATGGAGGGGTGCCAAAAGAATACTTCATCGAGTTACTTCTGAAAGCTTTAGAAGAGACTCGTGACGTTTATAGCAATAAACGTGCAGCATTCAGAGTTGCCCTTAGATATGGCGAGATCGACGACAATAGTAATGTTGCGAAAATGATCTCCTGTGGTATTCCTCTTGAAGAACCATATGTGCAATATCGTCTTTCAGTCCTGATGAACATGGAAAAGATCAATCTTAAAAAAGGGAAAATTTGTATTCCTGAATCATACTATTTGATGGGGACAGCTGATCCAACAGGAAAACTTGAAAAAGATCAAGTTTGCATTATCCTAAAAGATGGACAACTGTCGGGAATGGTTCTAGTGTACCGGAATCCTGGTGTACACTTCGGTGATATTCATGTCCTAAAAGCCACGTATGTCAAGGAACTAGAATCTGTTGTTGGAAATGCCAAGTACGGTATATTCTTCTCTTGTAAAGGTCCACGTTCCGTTGCGGATGAAATGGGTGGTGGAGATTTTGATGGTGATCTATACTGGATCTCAAGAAACCCTCAGCTGTTGGAATCGTTTAAACCAAGTGAACCTTGGATTGAAAAGTCATCTACCCAAAAAGTTCATGGTAAAAGACCAACCGAGCTTTCAAATGTTGATCTAGAAGATGAGCTCATCAAACTATTCTTGAAGACTAGGTTTGAACCAAGCTTCGCAATGAGTGAGGCAGCTCAACACTGGATGGCTTGGATGGACAAATTTTTGAGTTTAGCAGATACGAGTATTGATGAGAAAAACGAACTGAAGGCAAAGATACTGCAATTGATTGATATTTACTATGAAGCTCTCGATGCACCCAAGAAAGGTTTTAAGGTTGTAGTTCCAAAAGAATTGAAGGTGAAGACGTTTCCACATTTCATGGAGAAGAAACCCCTAGATTCCTACCATTCAATTTCCATTTTGGGATCAATTTATGATAAGGTGGATGAATATGAAACAGAAGACAGATCTCTCAAAGAAATTCAGAAACTTCCTCTGTTTGAAGTTGACGTTCCTGAGGGTAGCTTGAAAGAATGGAAGAAACTATACCAAGATTACCGGAATGAGATGACCTCTGCCATGGGAGCCGAAGATAAACAGGGCCAGAATCAGGCAGCCAATATCGTCATAAGAAAGTATAAAGAGATCCTATACAAGGCTGAGGAGTTTGAGCAAAGTGATATGCGGCACAGACAACAAATATATAATGACGCACTCGCTATTTATCACGTTTGTTATGACTATGCCAAGAGTGTTAGGACTGAAACGGGTGCACAAGATGTCAAGAAATGTGGCTTTGCTTGGAAAGTTGCAGGTGCAGCTCTATGCCAGCTTCACGAAGACTGGAGTTTGAAACCTGGTGAAAAGGTTTTCAGAGTCTTACCCACTGTTTTGAAGGACATTATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RdRP

Protein Analysis

854

Amino Acids

97.34

Weight (kDa)

6.28

Isoelectric Point (pI)

37.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RdRP PF05183 67 - 700 1.5e-99 RNA dependent RNA polymerase
RdRP_head PF26253 728 - 832 2.7e-09 RdRP, head domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000505)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G19910 AT2G19910 AT2G19920 AT2G19920 AT2G19920 AT2G19920 AT2G19930 AT2G19930 AT2G19930
fragaria_vesca FvH4_1g05980 FvH4_1g05990 FvH4_1g05990 FvH4_1g09718
malus_domestica MD02G1064400.v1.1 MD15G1195300.v1.1 MD15G1195400.v1.1
prunus_persica Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1
pyrus_communis pycom02g05070 pycom15g17460
rosa_chinensis RchiOBHm_Chr2g0091671 RchiOBHm_Chr2g0091681 RchiOBHm_Chr2g0091691 RchiOBHm_Chr2g0091701 RchiOBHm_Chr2g0091711 RchiOBHm_Chr2g0091721 RchiOBHm_Chr2g0091731 RchiOBHm_Chr5g0014401
rosa_laevigata RLG00000013772 RLG00000016242 RLG00000016244 RLG00000016245 RLG00000016246 RLG00000032105
rosa_multiflora Rmu_co8374041.1_g000001 Rmu_co8478121.1_g000001 Rmu_sc0000897.1_g000005 Rmu_sc0000897.1_g000009
rosa_roxburghii Rroxscaffold_1G00062180 Rroxscaffold_1G00062190 Rroxscaffold_1G00068800 Rroxscaffold_2G00149790 Rroxscaffold_2G00149800 Rroxscaffold_2G00149810
rosa_rugosa Rorug02G0019600 Rorug02G0019700 Rorug02G0019800 Rorug02G0019900 Rorug02G0019900 Rorug02G0020000 Rorug02G0020000 Rorug02G0020100 Rorug02G0020100 Rorug02G0020200 Rorug05G0017800
rosa_samantha Rh2AG064700 Rh2AG064800 Rh2AG064900 Rh2BG063800 Rh2BG063900 Rh2BG064000 Rh2BG064100 Rh2CG065500 Rh2CG065600 Rh2CG065700 Rh2DG063900 Rh2DG064000 Rh2DG064100 Rh2DG064200 Rh2DG381700 Rh5AG056700 Rh5AG056800 Rh5AG056900 Rh5AG112600 Rh5BG109300 Rh5CG063800 Rh5CG121100 Rh5DG053100 Rh5DG108300 Rh6CG100100
rosa_wichuraiana Rw2G005560 Rw2G005580 Rw2G005590 Rw5G005370 Rw5G009770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 507
AarI CACCTGC 1 cut(s) 2459
Acc36I ACCTGC 1 cut(s) 2459
AccB1I GGYRCC 1 cut(s) 1141
AccI GTMKAC 1 cut(s) 724
AciI CCGC 5 cut(s) 71, 370, 755, 1619, 2341
AclWI GGATC 9 cut(s) 51, 765, 827, 1019, 1034, 1406, 1670, 2089, 2296
AcsI RAATTY 6 cut(s) 278, 500, 1054, 1371, 1874, 2136
AcuI CTGAAG 1 cut(s) 1940
AcyI GRCGYC 1 cut(s) 123
AdeI CACNNNGTG 1 cut(s) 388
AfaI GTAC 5 cut(s) 328, 558, 1490, 1506, 1580
AfiI CCNNNNNNNGG 6 cut(s) 83, 189, 1138, 1979, 2266, 2311
AflII CTTAAG 1 cut(s) 59
AjnI CCWGG 3 cut(s) 877, 1498, 2512
AjuI GAANNNNNNNTTGG 4 cut(s) 1548, 1580, 1747, 1779
AleI CACNNNNGTG 1 cut(s) 1512
Alw21I GWGCWC 4 cut(s) 393, 476, 1791, 2431
Alw26I GTCTC 2 cut(s) 683, 1181
Alw44I GTGCAC 2 cut(s) 389, 2427
AlwI GGATC 9 cut(s) 51, 765, 827, 1019, 1034, 1406, 1670, 2089, 2296
AlwNI CAGNNNCTG 1 cut(s) 898
AoxI GGCC 2 cut(s) 566, 2257
ApaLI GTGCAC 2 cut(s) 389, 2427
ApeKI GCWGC 8 cut(s) 13, 31, 73, 482, 1217, 1844, 2270, 2474
ApoI RAATTY 6 cut(s) 278, 500, 1054, 1371, 1874, 2136
Asp700I GAANNNNTTC 1 cut(s) 354
AspS9I GGNCC 4 cut(s) 209, 566, 1604, 2257
AsuHPI GGTGA 7 cut(s) 47, 286, 435, 1526, 1661, 2026, 2528
AsuNHI GCTAGC 1 cut(s) 511
AvaII GGWCC 2 cut(s) 209, 1604
AxyI CCTNAGG 1 cut(s) 2172
BaeGI GKGCMC 2 cut(s) 393, 2431
BanI GGYRCC 1 cut(s) 1141
BanII GRGCYC 2 cut(s) 476, 1791
BarI GAAGNNNNNNTAC 2 cut(s) 2186, 2218
BauI CACGAG 1 cut(s) 1191
BbsI GAAGAC 4 cut(s) 1814, 2024, 2124, 2502
Bbv12I GWGCWC 4 cut(s) 393, 476, 1791, 2431
BbvCI CCTCAGC 2 cut(s) 1677, 2316
BbvI GCAGC 8 cut(s) 25, 43, 85, 494, 1229, 1856, 2282, 2486
BccI CCATC 9 cut(s) 182, 704, 707, 728, 1393, 1454, 1640, 1852, 1861
BceAI ACGGC 1 cut(s) 100
BcgI CGANNNNNNTGC 2 cut(s) 343, 377
BciT130I CCWGG 3 cut(s) 879, 1500, 2514
BcoDI GTCTC 2 cut(s) 683, 1181
BfmI CTRYAG 1 cut(s) 239
BfrI CTTAAG 1 cut(s) 59
BfuAI ACCTGC 1 cut(s) 2459
BglII AGATCT 2 cut(s) 539, 2123
Bme1390I CCNGG 3 cut(s) 879, 1500, 2514
Bme18I GGWCC 2 cut(s) 209, 1604
BmgT120I GGNCC 4 cut(s) 209, 566, 1604, 2257
BmiI GGNNCC 5 cut(s) 210, 211, 298, 1143, 2242
BmrFI CCNGG 3 cut(s) 879, 1500, 2514
BmrI ACTGGG 1 cut(s) 573
BmsI GCATC 4 cut(s) 96, 469, 516, 1957
BmtI GCTAGC 1 cut(s) 515
BmuI ACTGGG 1 cut(s) 573
BoxI GACNNNNGTC 1 cut(s) 1468
BpiI GAAGAC 4 cut(s) 1814, 2024, 2124, 2502
BplI GAGNNNNNCTC 2 cut(s) 2213, 2245
BpmI CTGGAG 2 cut(s) 1082, 2521
Bpu10I CCTNAGC 2 cut(s) 1677, 2316
BpuEI CTTGAG 2 cut(s) 869, 1651
BsaAI YACGTR 2 cut(s) 556, 1539
BsaBI GATNNNNATC 2 cut(s) 748, 1650
BsaHI GRCGYC 1 cut(s) 123
BsaJI CCNNGG 5 cut(s) 183, 286, 1132, 1709, 2235
BsaWI WCCGGW 2 cut(s) 1491, 2214
Bsc4I CCNNNNNNNGG 6 cut(s) 83, 189, 1138, 1979, 2266, 2311
Bse1I ACTGG 6 cut(s) 300, 568, 1065, 1664, 1859, 2504
Bse21I CCTNAGG 1 cut(s) 2172
Bse3DI GCAATG 3 cut(s) 104, 986, 1839
Bse8I GATNNNNATC 2 cut(s) 748, 1650
BseBI CCWGG 3 cut(s) 879, 1500, 2514
BseDI CCNNGG 5 cut(s) 183, 286, 1132, 1709, 2235
BseGI GGATG 5 cut(s) 193, 1627, 1863, 1872, 2107
BseJI GATNNNNATC 2 cut(s) 748, 1650
BseLI CCNNNNNNNGG 6 cut(s) 83, 189, 1138, 1979, 2266, 2311
BseMI GCAATG 3 cut(s) 104, 986, 1839
BseMII CTCAG 6 cut(s) 101, 768, 905, 1691, 2163, 2307
BseNI ACTGG 6 cut(s) 300, 568, 1065, 1664, 1859, 2504
BseRI GAGGAG 1 cut(s) 2333
BseSI GKGCMC 2 cut(s) 393, 2431
BseXI GCAGC 8 cut(s) 25, 43, 85, 494, 1229, 1856, 2282, 2486
BsgI GTGCAG 4 cut(s) 32, 50, 1236, 2493
Bsh1285I CGRYCG 1 cut(s) 1001
BshFI GGCC 2 cut(s) 568, 2259
BshNI GGYRCC 1 cut(s) 1141
BsiEI CGRYCG 1 cut(s) 1001
BsiHKAI GWGCWC 4 cut(s) 393, 476, 1791, 2431
BsiSI CCGG 3 cut(s) 77, 1492, 2215
BslFI GGGAC 3 cut(s) 195, 837, 1417
BslI CCNNNNNNNGG 6 cut(s) 83, 189, 1138, 1979, 2266, 2311
BsmAI GTCTC 2 cut(s) 683, 1181
BsmFI GGGAC 3 cut(s) 195, 837, 1417
BsmI GAATGC 2 cut(s) 354, 1220
BsnI GGCC 2 cut(s) 568, 2259
Bsp1286I GDGCHC 4 cut(s) 393, 476, 1791, 2431
Bsp1407I TGTACA 1 cut(s) 1504
Bsp19I CCATGG 2 cut(s) 1132, 2235
BspACI CCGC 5 cut(s) 71, 370, 755, 1619, 2341
BspANI GGCC 2 cut(s) 568, 2259
BspCNI CTCAG 6 cut(s) 102, 769, 904, 1690, 2164, 2308
BspLI GGNNCC 5 cut(s) 210, 211, 298, 1143, 2242
BspMI ACCTGC 1 cut(s) 2459
BspOI GCTAGC 1 cut(s) 515
BspPI GGATC 9 cut(s) 51, 765, 827, 1019, 1034, 1406, 1670, 2089, 2296
BspT107I GGYRCC 1 cut(s) 1141
BspTI CTTAAG 1 cut(s) 59
BsrDI GCAATG 3 cut(s) 104, 986, 1839
BsrGI TGTACA 1 cut(s) 1504
BsrI ACTGG 6 cut(s) 300, 568, 1065, 1664, 1859, 2504
BssECI CCNNGG 5 cut(s) 183, 286, 1132, 1709, 2235
BssNAI GTATAC 1 cut(s) 725
BssNI GRCGYC 1 cut(s) 123
BssSI CACGAG 1 cut(s) 1191
BssT1I CCWWGG 4 cut(s) 286, 1132, 1709, 2235
Bst1107I GTATAC 1 cut(s) 725
Bst2BI CACGAG 1 cut(s) 1191
Bst2UI CCWGG 3 cut(s) 879, 1500, 2514
Bst4CI ACNGT 9 cut(s) 395, 619, 677, 1002, 1035, 1051, 1470, 1583, 2545
Bst6I CTCTTC 1 cut(s) 1179
BstACI GRCGYC 1 cut(s) 123
BstAFI CTTAAG 1 cut(s) 59
BstAUI TGTACA 1 cut(s) 1504
BstBAI YACGTR 2 cut(s) 556, 1539
BstC8I GCNNGC 5 cut(s) 11, 102, 513, 517, 2486
BstDEI CTNAG 8 cut(s) 110, 777, 891, 1089, 1235, 1677, 2172, 2316
BstDSI CCRYGG 3 cut(s) 183, 1132, 2235
BstENI CCTNNNNNAGG 1 cut(s) 2309
BstF5I GGATG 5 cut(s) 193, 1627, 1863, 1872, 2107
BstMAI GTCTC 2 cut(s) 683, 1181
BstMCI CGRYCG 1 cut(s) 1001
BstMWI GCNNNNNNNGC 8 cut(s) 37, 73, 106, 115, 761, 1967, 2240, 2474
BstNI CCWGG 3 cut(s) 879, 1500, 2514
BstPAI GACNNNNGTC 1 cut(s) 1468
BstSCI CCNGG 3 cut(s) 877, 1498, 2512
BstSFI CTRYAG 1 cut(s) 239
BstSLI GKGCMC 2 cut(s) 393, 2431
BstV1I GCAGC 8 cut(s) 25, 43, 85, 494, 1229, 1856, 2282, 2486
BstV2I GAAGAC 4 cut(s) 1814, 2024, 2124, 2502
BstX2I RGATCY 7 cut(s) 539, 757, 1024, 1039, 1662, 2123, 2301
BstYI RGATCY 7 cut(s) 539, 757, 1024, 1039, 1662, 2123, 2301
BstZ17I GTATAC 1 cut(s) 725
Bsu36I CCTNAGG 1 cut(s) 2172
BsuRI GGCC 2 cut(s) 568, 2259
BtgI CCRYGG 3 cut(s) 183, 1132, 2235
BtsCI GGATG 5 cut(s) 193, 1627, 1863, 1872, 2107
BtsIMutI CAGTG 6 cut(s) 400, 561, 1056, 1058, 1852, 2541
BveI ACCTGC 1 cut(s) 2459
Cac8I GCNNGC 5 cut(s) 11, 102, 513, 517, 2486
CaiI CAGNNNCTG 1 cut(s) 898
Cfr13I GGNCC 4 cut(s) 209, 566, 1604, 2257
CseI GACGC 3 cut(s) 131, 559, 2375
CsiI ACCWGGT 1 cut(s) 2512
Csp6I GTAC 5 cut(s) 327, 557, 1489, 1505, 1579
CspCI CAANNNNNGTGG 4 cut(s) 152, 187, 2428, 2463
CviQI GTAC 5 cut(s) 327, 557, 1489, 1505, 1579
DdeI CTNAG 8 cut(s) 110, 777, 891, 1089, 1235, 1677, 2172, 2316
DraI TTTAAA 1 cut(s) 1696
DraIII CACNNNGTG 1 cut(s) 388
Eam1104I CTCTTC 1 cut(s) 1179
EarI CTCTTC 1 cut(s) 1179
Ecl136II GAGCTC 2 cut(s) 474, 1789
Eco130I CCWWGG 4 cut(s) 286, 1132, 1709, 2235
Eco24I GRGCYC 2 cut(s) 476, 1791
Eco47I GGWCC 2 cut(s) 209, 1604
Eco53kI GAGCTC 2 cut(s) 474, 1789
Eco57I CTGAAG 1 cut(s) 1940
Eco81I CCTNAGG 1 cut(s) 2172
EcoICRI GAGCTC 2 cut(s) 474, 1789
EcoNI CCTNNNNNAGG 1 cut(s) 2309
EcoO109I RGGNCCY 1 cut(s) 209
EcoRI GAATTC 1 cut(s) 1054
EcoRII CCWGG 3 cut(s) 877, 1498, 2512
EcoT14I CCWWGG 4 cut(s) 286, 1132, 1709, 2235
EcoT22I ATGCAT 1 cut(s) 531
EcoT38I GRGCYC 2 cut(s) 476, 1791
ErhI CCWWGG 4 cut(s) 286, 1132, 1709, 2235
FalI AAGNNNNNCTT 8 cut(s) 197, 229, 342, 374, 783, 815, 1693, 1725
FaqI GGGAC 3 cut(s) 195, 837, 1417
FauNDI CATATG 1 cut(s) 1309
FblI GTMKAC 1 cut(s) 724
FokI GGATG 5 cut(s) 200, 1634, 1870, 1879, 2114
FriOI GRGCYC 2 cut(s) 476, 1791
GsuI CTGGAG 2 cut(s) 1082, 2521
HaeIII GGCC 2 cut(s) 568, 2259
HapII CCGG 3 cut(s) 77, 1492, 2215
HgaI GACGC 3 cut(s) 131, 559, 2375
Hin1I GRCGYC 1 cut(s) 123
HincII GTYRAC 1 cut(s) 2164
HindII GTYRAC 1 cut(s) 2164
HindIII AAGCTT 3 cut(s) 717, 1176, 1825
HpaII CCGG 3 cut(s) 77, 1492, 2215
HphI GGTGA 7 cut(s) 47, 286, 435, 1526, 1661, 2026, 2528
Hpy188I TCNGA 9 cut(s) 92, 139, 478, 920, 1039, 1173, 1226, 2142, 2531
Hpy99I CGWCG 2 cut(s) 86, 1256
HpyAV CCTTC 7 cut(s) 223, 287, 344, 527, 1915, 2005, 2545
HpyCH4III ACNGT 9 cut(s) 395, 619, 677, 1002, 1035, 1051, 1470, 1583, 2545
HpyCH4IV ACGT 8 cut(s) 555, 1197, 1213, 1538, 1609, 2021, 2166, 2385
HpyF10VI GCNNNNNNNGC 8 cut(s) 37, 73, 106, 115, 761, 1967, 2240, 2474
HpyF3I CTNAG 8 cut(s) 110, 777, 891, 1089, 1235, 1677, 2172, 2316
HpySE526I ACGT 8 cut(s) 555, 1197, 1213, 1538, 1609, 2021, 2166, 2385
Hsp92I GRCGYC 1 cut(s) 123
KflI GGGWCCC 1 cut(s) 209
LmnI GCTCC 1 cut(s) 2240
Lsp1109I GCAGC 8 cut(s) 25, 43, 85, 494, 1229, 1856, 2282, 2486
LweI GCATC 4 cut(s) 96, 469, 516, 1957
MabI ACCWGGT 1 cut(s) 2512
MaeII ACGT 8 cut(s) 555, 1197, 1213, 1538, 1609, 2021, 2166, 2385
MaeIII GTNAC 8 cut(s) 53, 380, 404, 455, 619, 639, 1164, 1193
MfeI CAATTG 1 cut(s) 1937
MflI RGATCY 7 cut(s) 539, 757, 1024, 1039, 1662, 2123, 2301
MhlI GDGCHC 4 cut(s) 393, 476, 1791, 2431
MlyI GAGTC 4 cut(s) 17, 695, 1183, 2541
MmeI TCCRAC 4 cut(s) 943, 1439, 1546, 1665
Mph1103I ATGCAT 1 cut(s) 531
MroXI GAANNNNTTC 1 cut(s) 354
MseI TTAA 5 cut(s) 60, 341, 1359, 1695, 1986
MslI CAYNNNNRTG 2 cut(s) 732, 1512
MspA1I CMGCKG 2 cut(s) 1409, 1681
MspCI CTTAAG 1 cut(s) 59
MspI CCGG 3 cut(s) 77, 1492, 2215
MspR9I CCNGG 3 cut(s) 879, 1500, 2514
MssI GTTTAAAC 1 cut(s) 1696
MunI CAATTG 1 cut(s) 1937
Mva1269I GAATGC 2 cut(s) 354, 1220
MvaI CCWGG 3 cut(s) 879, 1500, 2514
MwoI GCNNNNNNNGC 8 cut(s) 37, 73, 106, 115, 761, 1967, 2240, 2474
NcoI CCATGG 2 cut(s) 1132, 2235
NdeI CATATG 1 cut(s) 1309
NheI GCTAGC 1 cut(s) 511
NlaIV GGNNCC 5 cut(s) 210, 211, 298, 1143, 2242
NmuCI GTSAC 4 cut(s) 53, 380, 639, 1193
NsiI ATGCAT 1 cut(s) 531
OliI CACNNNNGTG 1 cut(s) 1512
PaqCI CACCTGC 1 cut(s) 2459
PctI GAATGC 2 cut(s) 354, 1220
PdmI GAANNNNTTC 1 cut(s) 354
PfeI GAWTC 8 cut(s) 146, 994, 1385, 1495, 1556, 1688, 2054, 2263
PleI GAGTC 4 cut(s) 17, 694, 1183, 2540
PmeI GTTTAAAC 1 cut(s) 1696
PpsI GAGTC 4 cut(s) 17, 694, 1183, 2540
Ppu21I YACGTR 2 cut(s) 556, 1539
PpuMI RGGWCCY 1 cut(s) 209
PshAI GACNNNNGTC 1 cut(s) 1468
PsiI TTATAA 1 cut(s) 507
Psp124BI GAGCTC 2 cut(s) 476, 1791
Psp5II RGGWCCY 1 cut(s) 209
Psp6I CCWGG 3 cut(s) 877, 1498, 2512
PspGI CCWGG 3 cut(s) 877, 1498, 2512
PspN4I GGNNCC 5 cut(s) 210, 211, 298, 1143, 2242
PspPI GGNCC 4 cut(s) 209, 566, 1604, 2257
PspPPI RGGWCCY 1 cut(s) 209
PstNI CAGNNNCTG 1 cut(s) 898
PsuI RGATCY 7 cut(s) 539, 757, 1024, 1039, 1662, 2123, 2301
PvuII CAGCTG 2 cut(s) 1409, 1681
RsaI GTAC 5 cut(s) 328, 558, 1490, 1506, 1580
RsaNI GTAC 5 cut(s) 327, 557, 1489, 1505, 1579
RseI CAYNNNNRTG 2 cut(s) 732, 1512
SacI GAGCTC 2 cut(s) 476, 1791
SaqAI TTAA 5 cut(s) 60, 341, 1359, 1695, 1986
Sau96I GGNCC 4 cut(s) 209, 566, 1604, 2257
SchI GAGTC 4 cut(s) 17, 695, 1183, 2541
ScrFI CCNGG 3 cut(s) 879, 1500, 2514
SduI GDGCHC 4 cut(s) 393, 476, 1791, 2431
SexAI ACCWGGT 1 cut(s) 2512
SfaNI GCATC 4 cut(s) 96, 469, 516, 1957
SfcI CTRYAG 1 cut(s) 239
SinI GGWCC 2 cut(s) 209, 1604
SmiMI CAYNNNNRTG 2 cut(s) 732, 1512
SmlI CTYRAG 3 cut(s) 59, 848, 1666
SmoI CTYRAG 3 cut(s) 59, 848, 1666
SsiI CCGC 5 cut(s) 71, 370, 755, 1619, 2341
SstI GAGCTC 2 cut(s) 476, 1791
StyD4I CCNGG 3 cut(s) 877, 1498, 2512
StyI CCWWGG 4 cut(s) 286, 1132, 1709, 2235
TaaI ACNGT 9 cut(s) 395, 619, 677, 1002, 1035, 1051, 1470, 1583, 2545
TaiI ACGT 8 cut(s) 558, 1200, 1216, 1541, 1612, 2024, 2169, 2388
TaqI TCGA 9 cut(s) 21, 46, 681, 819, 997, 1106, 1161, 1251, 1965
TatI WGTACW 1 cut(s) 1504
TauI GCSGC 2 cut(s) 373, 2344
TfiI GAWTC 8 cut(s) 146, 994, 1385, 1495, 1556, 1688, 2054, 2263
Tru1I TTAA 5 cut(s) 60, 341, 1359, 1695, 1986
Tru9I TTAA 5 cut(s) 60, 341, 1359, 1695, 1986
TscAI CASTG 6 cut(s) 400, 568, 1056, 1065, 1859, 2548
TseFI GTSAC 4 cut(s) 53, 380, 639, 1193
TseI GCWGC 8 cut(s) 13, 31, 73, 482, 1217, 1844, 2270, 2474
Tsp45I GTSAC 4 cut(s) 53, 380, 639, 1193
TspGWI ACGGA 3 cut(s) 16, 172, 1603
TspRI CASTG 6 cut(s) 400, 568, 1056, 1065, 1859, 2548
Vha464I CTTAAG 1 cut(s) 59
VneI GTGCAC 2 cut(s) 389, 2427
VpaK11BI GGWCC 2 cut(s) 209, 1604
XagI CCTNNNNNAGG 1 cut(s) 2309
XapI RAATTY 6 cut(s) 278, 500, 1054, 1371, 1874, 2136
XbaI TCTAGA 1 cut(s) 1777
XcmI CCANNNNNNNNNTGG 2 cut(s) 1707, 1739
XmiI GTMKAC 1 cut(s) 724
XmnI GAANNNNTTC 1 cut(s) 354
Zsp2I ATGCAT 1 cut(s) 531
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.