Rmu_sc0000897.1_g000009
ERF Family

Probably involved in the RNA silencing pathway and required for the generation of small interfering RNAs (siRNAs)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000897.1
OG0000505
Physical Location & Seq
Reverse (-)
40468 .. 50773
10306 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000897.1_g000009.1.cds

Sequence Viewer

Length: 3639 bp
atgatgaagcagagttgggatgaacaagaagaggtgccgcgtctacctcagaaagtggaggtattgctccggcaaatctgtgaggacaaaaagcaaccttggccggacgctgacgccataaggcagctcgcggctcagagcgaggaggcggcaatggagaccctgaagaaaatctccaccagcaaaagcatcagaaccaccctcggccgttacatcaagtggatgatcgggcaatgtagctctgcttctccgtcgccgtccaaaacggtgcgttccgctcacgttcaaagccctggtgctacttcactcactcctcagagacttcacttcagccctccaggtgaggcagttagtgacaggtcacctgttgttgggcaattaccttattgctctggttctccatccaaagccgcggggacttgttcttatcaaagattgtctgctcatgcacaggttgatgagaggtctcgaggcgaggcatttaatcagctgtcacctgttattggtcaatcaccttattcctctggttctcctttgaaagccgcagacacttcttcttatcaaagactgtctgctcctgtacaactggatgagagatttcaaggtgatgcatttagtcattggtcatctgttggacaaccaccttattcctctgcttctccatcaaaagctgcaggcactacttcttaccaaagattgtctcctcctgtacaacttgatgatacgtttcaaggggaggcatttactgttctagagcaaccaggaaacatcaataatggacaatcaccttattattcctctggttctccattgaaagtggcacgcacttgttcgtgtcaaagaccatctgcgttggttgctcctatacaacttgataagaagtttcaaggtgacgcatttactgacaggtcacttgttcaacaattaccttgttcctctggttctccattaaaagccactggttcttatgaaagattgtctactcctgcacaagctgatgagcggtttcatggtgaggcatttagtgcccggtcagctgttggacaattgccttgttcctctgtttctccattaaaatctgcagtcacttcttcttatcaaagattgtctactcctgtacaacttgatgagaggtttcaagttgaggcatttaatgaccggtcacctgttattggacaatcaccttactctggttctccattgaaagccgcaagcacttcttatcaaagactgactgctcctctacaacttgatgagagggttcaaggtggggtatttgctgatcagacacctgttcaaggacaatcaccatatgcctcttgttctccatcaaaagccttctgcatttctccttatcagagaatgtctgctgatgttgttcctgtacaacttcatgaaaggtttcaaggtgagggtatcactgggtcctcagctatagacaaacctggagatcatggagaagcacacttagtagctctgggtgaactagaattccgtaaacaatttctgattttaagttatgctggaggaacgaagttgagagatcttatagcggcagaggacattagggattggaaatatttgccaatggttgcaagagaagggattcttgttggtttgcggcgatatcgtttctttgtgttcaaagatggtggtaatgaggagaagaaaaaaaacccaacttcatcacctgtgaagtgttactttgttcgagtgacctctgatgcagccatagacaaaggtgtcagttatatattatctgacagaacgatgtatgaagccagatgtcttttcatgcatgcacatactctggctagtattgataactacatggttaggttctcactcattttgtccaagacaacgagcttaaaagtagattggtcagttgtcaaggttgaaatcatcggtgaagaatactgtctcgatgaatctggtaatcgtatccatagagatgaaaaaccacttatacatacagatggaactggattcatatccgaggatttggctttaatttgtccaaagatagaagccaaaggacaatgtataagtgatgaacacatgaagggacttcctgatcctgatgaacttgaggataatgatatgggaaagaaaaggccagttcttagaacagaagaaccgcctttgctcacacagtgccgactttttcacaatggcagtgcttctaaaggaacgtttcttgtcaataaaaagctccatccgaacacaatccagattcgaccatccatgctcaaagttaagacagatccaacaatttccaataatcaaacaataaactcattggaaatagttggcacaagcactcctcctaggaatacgcacttatcgaggattttaattgcacttctaatgcatggaggggtaccggaagaatttttcatgcagatacttaaccaagctttggaagatgctcatggtgttttctgcaagacacgtgcagcactcaaagttgctgagaagtatggtgagattgatgacaatttcttggggaaattgatgatctcatttggaatacctctagaagaatcatacttgcggaaggatggacaactgtcggggaaggtactagtctacagatatccgggtttacactttggggatattcatgttctaaaggcgacctatgtgaaggagttagaatcttttgttggaaatgccaaatacggtatattcttctcttgcaaaggtccacgttccattgctgatgaaatgggcggtggagattttgatggtgatctatactgggtctcaagaaaccctcagctattggagtggtacaaaccaagtgaaccttggattgaagcttcaataccaacccaaaaagttcccagtaggagtccatctgactattcgcctgaagatctagaagatcttctgtttagatcattcttgacaacgaggtttgaaccaagtttcgctatgagtgaggcagctgataattggctagcctggatggatcgtttcttgacgttgggagagagtgacattgacgaaaagaataatatgaagatgaagatactgcaactaattaacttatactacacagctctagatgcaccgaagaaaggtgtaaaggttgtagtcccaaaagaattgaaggccccatgtttcccccattacatgggaaagaacaattcttacccttcaacttccattctgggattaatttataacagggtggatgaggatcaaacagaagaccgttctctcaaagaagtcgagaaacttcctgcttttgatgttaatgaagtccctgatgcctgcttggacaaatgggaggaactacaccgacagtacaggagtgagatggcctctgctatggacgatgctgataaacagagcaaaaatcagtctgccgatgtggtcataaggaaatatagaaagattctttacgaggctaatgactttgaggagagtagaaggccaatcgatgagatatataacgaggcacaagctatatatcatgtgtgttatgaccatgcgaggaggataggaagtgtgaaggcgtgttcctttgcatggagagttgcaggtaaagctctgtgccagcttcacgccaagaacacgaatgaaaggtgttgtacgatagtatcctctgttttgaaggaaattttgtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RdRP

Protein Analysis

1212

Amino Acids

134.91

Weight (kDa)

6.45

Isoelectric Point (pI)

48.6

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000505)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G19910 AT2G19910 AT2G19920 AT2G19920 AT2G19920 AT2G19920 AT2G19930 AT2G19930 AT2G19930
fragaria_vesca FvH4_1g05980 FvH4_1g05990 FvH4_1g05990 FvH4_1g09718
malus_domestica MD02G1064400.v1.1 MD15G1195300.v1.1 MD15G1195400.v1.1
prunus_persica Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1
pyrus_communis pycom02g05070 pycom15g17460
rosa_chinensis RchiOBHm_Chr2g0091671 RchiOBHm_Chr2g0091681 RchiOBHm_Chr2g0091691 RchiOBHm_Chr2g0091701 RchiOBHm_Chr2g0091711 RchiOBHm_Chr2g0091721 RchiOBHm_Chr2g0091731 RchiOBHm_Chr5g0014401
rosa_laevigata RLG00000013772 RLG00000016242 RLG00000016244 RLG00000016245 RLG00000016246 RLG00000032105
rosa_multiflora Rmu_co8374041.1_g000001 Rmu_co8478121.1_g000001 Rmu_sc0000897.1_g000005 Rmu_sc0000897.1_g000009
rosa_roxburghii Rroxscaffold_1G00062180 Rroxscaffold_1G00062190 Rroxscaffold_1G00068800 Rroxscaffold_2G00149790 Rroxscaffold_2G00149800 Rroxscaffold_2G00149810
rosa_rugosa Rorug02G0019600 Rorug02G0019700 Rorug02G0019800 Rorug02G0019900 Rorug02G0019900 Rorug02G0020000 Rorug02G0020000 Rorug02G0020100 Rorug02G0020100 Rorug02G0020200 Rorug05G0017800
rosa_samantha Rh2AG064700 Rh2AG064800 Rh2AG064900 Rh2BG063800 Rh2BG063900 Rh2BG064000 Rh2BG064100 Rh2CG065500 Rh2CG065600 Rh2CG065700 Rh2DG063900 Rh2DG064000 Rh2DG064100 Rh2DG064200 Rh2DG381700 Rh5AG056700 Rh5AG056800 Rh5AG056900 Rh5AG112600 Rh5BG109300 Rh5CG063800 Rh5CG121100 Rh5DG053100 Rh5DG108300 Rh6CG100100
rosa_wichuraiana Rw2G005560 Rw2G005580 Rw2G005590 Rw5G005370 Rw5G009770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3192
AasI GACNNNNNNGTC 1 cut(s) 1742
Acc36I ACCTGC 1 cut(s) 3542
Acc65I GGTACC 1 cut(s) 2383
AccB1I GGYRCC 2 cut(s) 34, 2383
AccB7I CCANNNNNTGG 2 cut(s) 2422, 3142
AccBSI CCGCTC 2 cut(s) 278, 1003
AccI GTMKAC 4 cut(s) 43, 980, 1109, 2592
AccII CGCG 3 cut(s) 40, 131, 413
AclI AACGTT 1 cut(s) 2195
AclWI GGATC 4 cut(s) 2072, 2261, 2985, 3216
AcoI YGGCCR 2 cut(s) 101, 205
AcsI RAATTY 3 cut(s) 1490, 2393, 3630
AcuI CTGAAG 3 cut(s) 185, 313, 2898
AcvI CACGTG 1 cut(s) 2456
AcyI GRCGYC 1 cut(s) 114
AdeI CACNNNGTG 1 cut(s) 2157
AfaI GTAC 9 cut(s) 582, 711, 1119, 1386, 2385, 2586, 2798, 3317, 3604
AfiI CCNNNNNNNGG 9 cut(s) 371, 503, 1172, 1190, 1298, 2422, 3086, 3142, 3540
AflIII ACRYGT 1 cut(s) 2453
AgeI ACCGGT 1 cut(s) 1158
AhlI ACTAGT 1 cut(s) 2587
AjnI CCWGG 5 cut(s) 292, 337, 760, 1444, 2969
AjuI GAANNNNNNNTTGG 2 cut(s) 2652, 2684
AloI GAACNNNNNNTCC 2 cut(s) 1476, 1508
Alw26I GTCTC 6 cut(s) 152, 313, 471, 705, 1928, 2773
AlwI GGATC 4 cut(s) 2072, 2261, 2985, 3216
Ama87I CYCGRG 1 cut(s) 468
AoxI GGCC 6 cut(s) 101, 205, 2117, 3120, 3330, 3443
ApeKI GCWGC 5 cut(s) 124, 671, 1727, 2459, 2951
ApoI RAATTY 3 cut(s) 1490, 2393, 3630
AseI ATTAAT 1 cut(s) 3185
AsiGI ACCGGT 1 cut(s) 1158
Asp700I GAANNNNTTC 3 cut(s) 1401, 1605, 2892
Asp718I GGTACC 1 cut(s) 2383
AspA2I CCTAGG 1 cut(s) 2330
AspS9I GGNCC 3 cut(s) 1425, 2708, 3121
AsuC2I CCSGG 2 cut(s) 1030, 2604
AsuNHI GCTAGC 1 cut(s) 2965
AvaI CYCGRG 1 cut(s) 468
AvaII GGWCC 2 cut(s) 1425, 2708
AvrII CCTAGG 1 cut(s) 2330
BaeGI GKGCMC 1 cut(s) 1030
BaeI ACNNNNGTAYC 2 cut(s) 3594, 3627
BanI GGYRCC 2 cut(s) 34, 2383
BarI GAAGNNNNNNTAC 4 cut(s) 229, 261, 2814, 2846
BbrPI CACGTG 1 cut(s) 2456
BbsI GAAGAC 1 cut(s) 3225
BbvCI CCTCAGC 2 cut(s) 1429, 2781
BbvI GCAGC 5 cut(s) 136, 658, 1739, 2471, 2963
BceAI ACGGC 2 cut(s) 192, 241
BciT130I CCWGG 5 cut(s) 294, 339, 762, 1446, 2971
BciVI GTATCC 2 cut(s) 1955, 3622
BclI TGATCA 1 cut(s) 1282
BcnI CCSGG 2 cut(s) 1030, 2604
BcoDI GTCTC 6 cut(s) 152, 313, 471, 705, 1928, 2773
BcuI ACTAGT 1 cut(s) 2587
BfaI CTAG 9 cut(s) 752, 1487, 1815, 2331, 2540, 2588, 2885, 2966, 3071
BfmI CTRYAG 4 cut(s) 672, 1080, 1434, 2593
BfuAI ACCTGC 1 cut(s) 3542
BfuI GTATCC 2 cut(s) 1955, 3622
BglII AGATCT 3 cut(s) 1543, 2881, 2890
BlnI CCTAGG 1 cut(s) 2330
Bme1390I CCNGG 7 cut(s) 294, 339, 762, 1030, 1446, 2604, 2971
Bme18I GGWCC 2 cut(s) 1425, 2708
BmeT110I CYCGRG 1 cut(s) 468
BmgT120I GGNCC 3 cut(s) 1425, 2708, 3121
BmiI GGNNCC 4 cut(s) 36, 1426, 2385, 3123
BmrFI CCNGG 7 cut(s) 294, 339, 762, 1030, 1446, 2604, 2971
BmrI ACTGGG 3 cut(s) 1431, 2773, 2844
BmsI GCATC 7 cut(s) 198, 598, 1714, 2419, 3064, 3268, 3337
BmtI GCTAGC 1 cut(s) 2969
BmuI ACTGGG 3 cut(s) 1431, 2773, 2844
BoxI GACNNNNGTC 1 cut(s) 2572
BpiI GAAGAC 1 cut(s) 3225
BplI GAGNNNNNCTC 2 cut(s) 3317, 3349
BpmI CTGGAG 3 cut(s) 321, 1467, 1545
Bpu10I CCTNAGC 2 cut(s) 1429, 2781
BpuEI CTTGAG 2 cut(s) 2111, 2755
BpuMI CCSGG 2 cut(s) 1030, 2604
Bsa29I ATCGAT 1 cut(s) 3450
BsaAI YACGTR 1 cut(s) 2456
BsaBI GATNNNNATC 3 cut(s) 1993, 2754, 3207
BsaHI GRCGYC 1 cut(s) 114
BsaI GGTCTC 3 cut(s) 152, 471, 2773
BsaJI CCNNGG 7 cut(s) 98, 202, 292, 411, 1998, 2330, 2813
BsaWI WCCGGW 2 cut(s) 1158, 2386
BsaXI ACNNNNNCTCC 2 cut(s) 2308, 2338
Bsc4I CCNNNNNNNGG 9 cut(s) 371, 503, 1172, 1190, 1298, 2422, 3086, 3142, 3540
Bse118I RCCGGY 1 cut(s) 1158
Bse1I ACTGG 7 cut(s) 591, 964, 1426, 1990, 2120, 2768, 2850
Bse3DI GCAATG 3 cut(s) 159, 239, 2718
Bse8I GATNNNNATC 3 cut(s) 1993, 2754, 3207
BseBI CCWGG 5 cut(s) 294, 339, 762, 1446, 2971
BseCI ATCGAT 1 cut(s) 3450
BseDI CCNNGG 7 cut(s) 98, 202, 292, 411, 1998, 2330, 2813
BseGI GGATG 9 cut(s) 25, 228, 401, 595, 2218, 2243, 2569, 2979, 3208
BseJI GATNNNNATC 3 cut(s) 1993, 2754, 3207
BseLI CCNNNNNNNGG 9 cut(s) 371, 503, 1172, 1190, 1298, 2422, 3086, 3142, 3540
BseMI GCAATG 3 cut(s) 159, 239, 2718
BseMII CTCAG 6 cut(s) 62, 149, 329, 1443, 2466, 2795
BseNI ACTGG 7 cut(s) 591, 964, 1426, 1990, 2120, 2768, 2850
BseRI GAGGAG 8 cut(s) 158, 303, 693, 1230, 1676, 2316, 3446, 3520
BseSI GKGCMC 1 cut(s) 1030
BseX3I CGGCCG 1 cut(s) 205
BseXI GCAGC 5 cut(s) 136, 658, 1739, 2471, 2963
BsgI GTGCAG 2 cut(s) 972, 2478
Bsh1236I CGCG 3 cut(s) 40, 131, 413
Bsh1285I CGRYCG 1 cut(s) 208
BshFI GGCC 6 cut(s) 103, 207, 2119, 3122, 3332, 3445
BshNI GGYRCC 2 cut(s) 34, 2383
BshTI ACCGGT 1 cut(s) 1158
BshVI ATCGAT 1 cut(s) 3450
BsiEI CGRYCG 1 cut(s) 208
BsiHKCI CYCGRG 1 cut(s) 468
BsiSI CCGG 6 cut(s) 70, 104, 1030, 1159, 2387, 2603
BslFI GGGAC 4 cut(s) 430, 2082, 3089, 3257
BslI CCNNNNNNNGG 9 cut(s) 371, 503, 1172, 1190, 1298, 2422, 3086, 3142, 3540
BsmAI GTCTC 6 cut(s) 152, 313, 471, 705, 1928, 2773
BsmFI GGGAC 4 cut(s) 430, 2082, 3089, 3257
BsnI GGCC 6 cut(s) 103, 207, 2119, 3122, 3332, 3445
Bso31I GGTCTC 3 cut(s) 152, 471, 2773
BsoBI CYCGRG 1 cut(s) 468
Bsp1286I GDGCHC 1 cut(s) 1030
Bsp1407I TGTACA 4 cut(s) 580, 709, 1117, 1384
BspANI GGCC 6 cut(s) 103, 207, 2119, 3122, 3332, 3445
BspCNI CTCAG 6 cut(s) 61, 148, 328, 1442, 2467, 2794
BspDI ATCGAT 1 cut(s) 3450
BspFNI CGCG 3 cut(s) 40, 131, 413
BspHI TCATGA 1 cut(s) 1393
BspLI GGNNCC 4 cut(s) 36, 1426, 2385, 3123
BspMAI CTGCAG 2 cut(s) 676, 1084
BspMI ACCTGC 1 cut(s) 3542
BspOI GCTAGC 1 cut(s) 2969
BspPI GGATC 4 cut(s) 2072, 2261, 2985, 3216
BspT107I GGYRCC 2 cut(s) 34, 2383
BspTNI GGTCTC 3 cut(s) 152, 471, 2773
BsrBI CCGCTC 2 cut(s) 278, 1003
BsrDI GCAATG 3 cut(s) 159, 239, 2718
BsrFI RCCGGY 1 cut(s) 1158
BsrGI TGTACA 4 cut(s) 580, 709, 1117, 1384
BsrI ACTGG 7 cut(s) 591, 964, 1426, 1990, 2120, 2768, 2850
BssAI RCCGGY 1 cut(s) 1158
BssECI CCNNGG 7 cut(s) 98, 202, 292, 411, 1998, 2330, 2813
BssNI GRCGYC 1 cut(s) 114
BssT1I CCWWGG 3 cut(s) 98, 2330, 2813
Bst2UI CCWGG 5 cut(s) 294, 339, 762, 1446, 2971
Bst4CI ACNGT 9 cut(s) 268, 570, 748, 1922, 2157, 2574, 2687, 3224, 3315
Bst6I CTCTTC 1 cut(s) 24
BstACI GRCGYC 1 cut(s) 114
BstAPI GCANNNNNTGC 1 cut(s) 1025
BstAUI TGTACA 4 cut(s) 580, 709, 1117, 1384
BstBAI YACGTR 1 cut(s) 2456
BstC8I GCNNGC 8 cut(s) 129, 676, 823, 1213, 1800, 2967, 3283, 3569
BstDEI CTNAG 8 cut(s) 48, 135, 315, 1429, 1468, 2126, 2475, 2781
BstDSI CCRYGG 1 cut(s) 411
BstEII GGTNACC 2 cut(s) 360, 1161
BstENI CCTNNNNNAGG 1 cut(s) 1296
BstF5I GGATG 9 cut(s) 25, 228, 401, 595, 2218, 2243, 2569, 2979, 3208
BstFNI CGCG 3 cut(s) 40, 131, 413
BstMAI GTCTC 6 cut(s) 152, 313, 471, 705, 1928, 2773
BstMCI CGRYCG 1 cut(s) 208
BstMWI GCNNNNNNNGC 6 cut(s) 100, 857, 1025, 1034, 3074, 3557
BstNI CCWGG 5 cut(s) 294, 339, 762, 1446, 2971
BstNSI RCATGY 1 cut(s) 1802
BstPAI GACNNNNGTC 1 cut(s) 2572
BstPI GGTNACC 2 cut(s) 360, 1161
BstSCI CCNGG 7 cut(s) 292, 337, 760, 1028, 1444, 2602, 2969
BstSFI CTRYAG 4 cut(s) 672, 1080, 1434, 2593
BstSLI GKGCMC 1 cut(s) 1030
BstUI CGCG 3 cut(s) 40, 131, 413
BstV1I GCAGC 5 cut(s) 136, 658, 1739, 2471, 2963
BstV2I GAAGAC 1 cut(s) 3225
BstX2I RGATCY 4 cut(s) 1543, 2266, 2881, 2890
BstYI RGATCY 4 cut(s) 1543, 2266, 2881, 2890
BstZI CGGCCG 1 cut(s) 205
Bsu15I ATCGAT 1 cut(s) 3450
BsuI GTATCC 2 cut(s) 1955, 3622
BsuRI GGCC 6 cut(s) 103, 207, 2119, 3122, 3332, 3445
BsuTUI ATCGAT 1 cut(s) 3450
BtgI CCRYGG 1 cut(s) 411
BtsCI GGATG 9 cut(s) 25, 228, 401, 595, 2218, 2243, 2569, 2979, 3208
BtsI GCAGTG 1 cut(s) 2185
BtsIMutI CAGTG 4 cut(s) 957, 1419, 2162, 2185
BveI ACCTGC 1 cut(s) 3542
Cac8I GCNNGC 8 cut(s) 129, 676, 823, 1213, 1800, 2967, 3283, 3569
CciI TCATGA 1 cut(s) 1393
Cfr10I RCCGGY 1 cut(s) 1158
Cfr13I GGNCC 3 cut(s) 1425, 2708, 3121
Cfr42I CCGCGG 1 cut(s) 414
ClaI ATCGAT 1 cut(s) 3450
CseI GACGC 4 cut(s) 29, 116, 122, 902
Csp6I GTAC 9 cut(s) 581, 710, 1118, 1385, 2384, 2585, 2797, 3316, 3603
CspAI ACCGGT 1 cut(s) 1158
CspCI CAANNNNNGTGG 2 cut(s) 1633, 1668
CviQI GTAC 9 cut(s) 581, 710, 1118, 1385, 2384, 2585, 2797, 3316, 3603
DdeI CTNAG 8 cut(s) 48, 135, 315, 1429, 1468, 2126, 2475, 2781
DraIII CACNNNGTG 1 cut(s) 2157
DrdI GACNNNNNNGTC 1 cut(s) 1742
DseDI GACNNNNNNGTC 1 cut(s) 1742
EaeI YGGCCR 2 cut(s) 101, 205
EagI CGGCCG 1 cut(s) 205
Eam1104I CTCTTC 1 cut(s) 24
EarI CTCTTC 1 cut(s) 24
EclXI CGGCCG 1 cut(s) 205
Eco130I CCWWGG 3 cut(s) 98, 2330, 2813
Eco31I GGTCTC 3 cut(s) 152, 471, 2773
Eco32I GATATC 2 cut(s) 1628, 2600
Eco47I GGWCC 2 cut(s) 1425, 2708
Eco52I CGGCCG 1 cut(s) 205
Eco57I CTGAAG 3 cut(s) 185, 313, 2898
Eco72I CACGTG 1 cut(s) 2456
Eco88I CYCGRG 1 cut(s) 468
Eco91I GGTNACC 2 cut(s) 360, 1161
EcoNI CCTNNNNNAGG 1 cut(s) 1296
EcoO109I RGGNCCY 2 cut(s) 1425, 3121
EcoO65I GGTNACC 2 cut(s) 360, 1161
EcoRI GAATTC 1 cut(s) 1490
EcoRII CCWGG 5 cut(s) 292, 337, 760, 1444, 2969
EcoRV GATATC 2 cut(s) 1628, 2600
EcoT14I CCWWGG 3 cut(s) 98, 2330, 2813
EcoT22I ATGCAT 3 cut(s) 613, 1800, 2376
ErhI CCWWGG 3 cut(s) 98, 2330, 2813
FalI AAGNNNNNCTT 8 cut(s) 1204, 1236, 1593, 1625, 1688, 1720, 2797, 2829
FaqI GGGAC 4 cut(s) 430, 2082, 3089, 3257
FauI CCCGC 1 cut(s) 406
FauNDI CATATG 1 cut(s) 1312
FbaI TGATCA 1 cut(s) 1282
FblI GTMKAC 4 cut(s) 43, 980, 1109, 2592
FokI GGATG 9 cut(s) 32, 235, 388, 602, 2205, 2230, 2576, 2986, 3215
FspBI CTAG 9 cut(s) 752, 1487, 1815, 2331, 2540, 2588, 2885, 2966, 3071
GsuI CTGGAG 3 cut(s) 321, 1467, 1545
HaeIII GGCC 6 cut(s) 103, 207, 2119, 3122, 3332, 3445
HapII CCGG 6 cut(s) 70, 104, 1030, 1159, 2387, 2603
HgaI GACGC 4 cut(s) 29, 116, 122, 902
Hin1I GRCGYC 1 cut(s) 114
HindIII AAGCTT 2 cut(s) 2418, 2823
HinfI GANTC 8 cut(s) 1606, 1931, 1989, 2236, 2546, 2660, 2857, 3406
HpaII CCGG 6 cut(s) 70, 104, 1030, 1159, 2387, 2603
Hpy166II GTNNAC 9 cut(s) 44, 981, 1110, 1484, 1499, 2593, 2609, 2711, 2810
Hpy8I GTNNAC 9 cut(s) 44, 981, 1110, 1484, 1499, 2593, 2609, 2711, 2810
Hpy99I CGWCG 1 cut(s) 256
HpyCH4III ACNGT 9 cut(s) 268, 570, 748, 1922, 2157, 2574, 2687, 3224, 3315
HpyCH4IV ACGT 6 cut(s) 282, 725, 2195, 2455, 2713, 2990
HpyF10VI GCNNNNNNNGC 6 cut(s) 100, 857, 1025, 1034, 3074, 3557
HpyF3I CTNAG 8 cut(s) 48, 135, 315, 1429, 1468, 2126, 2475, 2781
HpySE526I ACGT 6 cut(s) 282, 725, 2195, 2455, 2713, 2990
Hsp92I GRCGYC 1 cut(s) 114
KpnI GGTACC 1 cut(s) 2387
Ksp22I TGATCA 1 cut(s) 1282
KspI CCGCGG 1 cut(s) 414
LmnI GCTCC 5 cut(s) 72, 580, 865, 1243, 2220
Lsp1109I GCAGC 5 cut(s) 136, 658, 1739, 2471, 2963
LweI GCATC 7 cut(s) 198, 598, 1714, 2419, 3064, 3268, 3337
MaeI CTAG 9 cut(s) 752, 1487, 1815, 2331, 2540, 2588, 2885, 2966, 3071
MaeII ACGT 6 cut(s) 282, 725, 2195, 2455, 2713, 2990
MbiI CCGCTC 2 cut(s) 278, 1003
MfeI CAATTG 1 cut(s) 1046
MflI RGATCY 4 cut(s) 1543, 2266, 2881, 2890
MhlI GDGCHC 1 cut(s) 1030
MlyI GAGTC 1 cut(s) 2866
MmeI TCCRAC 4 cut(s) 613, 1021, 2294, 2650
Mph1103I ATGCAT 3 cut(s) 613, 1800, 2376
MroXI GAANNNNTTC 3 cut(s) 1401, 1605, 2892
MslI CAYNNNNRTG 2 cut(s) 1953, 1977
MspA1I CMGCKG 4 cut(s) 413, 490, 1037, 2954
MspI CCGG 6 cut(s) 70, 104, 1030, 1159, 2387, 2603
MspR9I CCNGG 7 cut(s) 294, 339, 762, 1030, 1446, 2604, 2971
MunI CAATTG 1 cut(s) 1046
MvaI CCWGG 5 cut(s) 294, 339, 762, 1446, 2971
MvnI CGCG 3 cut(s) 40, 131, 413
MwoI GCNNNNNNNGC 6 cut(s) 100, 857, 1025, 1034, 3074, 3557
NciI CCSGG 2 cut(s) 1030, 2604
NdeI CATATG 1 cut(s) 1312
NheI GCTAGC 1 cut(s) 2965
NlaIV GGNNCC 4 cut(s) 36, 1426, 2385, 3123
NmeAIII GCCGAG 1 cut(s) 183
NmuCI GTSAC 9 cut(s) 353, 360, 492, 890, 909, 1084, 1161, 1714, 3002
NsiI ATGCAT 3 cut(s) 613, 1800, 2376
NspI RCATGY 1 cut(s) 1802
PaeI GCATGC 1 cut(s) 1802
PaeR7I CTCGAG 1 cut(s) 468
PagI TCATGA 1 cut(s) 1393
PcsI WCGNNNNNNNCGW 1 cut(s) 2345
PdmI GAANNNNTTC 3 cut(s) 1401, 1605, 2892
PfeI GAWTC 7 cut(s) 1606, 1931, 1989, 2236, 2546, 2660, 3406
PflMI CCANNNNNTGG 2 cut(s) 2422, 3142
PinAI ACCGGT 1 cut(s) 1158
PleI GAGTC 1 cut(s) 2865
PmaCI CACGTG 1 cut(s) 2456
PmlI CACGTG 1 cut(s) 2456
PpsI GAGTC 1 cut(s) 2865
Ppu21I YACGTR 1 cut(s) 2456
PpuMI RGGWCCY 1 cut(s) 1425
PshAI GACNNNNGTC 1 cut(s) 2572
PshBI ATTAAT 1 cut(s) 3185
PsiI TTATAA 1 cut(s) 3192
Psp1406I AACGTT 1 cut(s) 2195
Psp5II RGGWCCY 1 cut(s) 1425
Psp6I CCWGG 5 cut(s) 292, 337, 760, 1444, 2969
PspCI CACGTG 1 cut(s) 2456
PspEI GGTNACC 2 cut(s) 360, 1161
PspGI CCWGG 5 cut(s) 292, 337, 760, 1444, 2969
PspN4I GGNNCC 4 cut(s) 36, 1426, 2385, 3123
PspPI GGNCC 3 cut(s) 1425, 2708, 3121
PspPPI RGGWCCY 1 cut(s) 1425
PsrI GAACNNNNNNTAC 2 cut(s) 3143, 3175
PstI CTGCAG 2 cut(s) 676, 1084
PsuI RGATCY 4 cut(s) 1543, 2266, 2881, 2890
PvuII CAGCTG 3 cut(s) 490, 1037, 2954
RsaI GTAC 9 cut(s) 582, 711, 1119, 1386, 2385, 2586, 2798, 3317, 3604
RsaNI GTAC 9 cut(s) 581, 710, 1118, 1385, 2384, 2585, 2797, 3316, 3603
RseI CAYNNNNRTG 2 cut(s) 1953, 1977
SacII CCGCGG 1 cut(s) 414
Sau96I GGNCC 3 cut(s) 1425, 2708, 3121
SchI GAGTC 1 cut(s) 2866
ScrFI CCNGG 7 cut(s) 294, 339, 762, 1030, 1446, 2604, 2971
SduI GDGCHC 1 cut(s) 1030
SfaNI GCATC 7 cut(s) 198, 598, 1714, 2419, 3064, 3268, 3337
SfcI CTRYAG 4 cut(s) 672, 1080, 1434, 2593
Sfr274I CTCGAG 1 cut(s) 468
Sfr303I CCGCGG 1 cut(s) 414
SgrBI CCGCGG 1 cut(s) 414
SinI GGWCC 2 cut(s) 1425, 2708
SlaI CTCGAG 1 cut(s) 468
SmiMI CAYNNNNRTG 2 cut(s) 1953, 1977
SmlI CTYRAG 3 cut(s) 468, 2090, 2770
SmoI CTYRAG 3 cut(s) 468, 2090, 2770
SpeI ACTAGT 1 cut(s) 2587
SphI GCATGC 1 cut(s) 1802
SspI AATATT 1 cut(s) 1580
SspMI CTAG 9 cut(s) 752, 1487, 1815, 2331, 2540, 2588, 2885, 2966, 3071
StyD4I CCNGG 7 cut(s) 292, 337, 760, 1028, 1444, 2602, 2969
StyI CCWWGG 3 cut(s) 98, 2330, 2813
TaaI ACNGT 9 cut(s) 268, 570, 748, 1922, 2157, 2574, 2687, 3224, 3315
TaiI ACGT 6 cut(s) 285, 728, 2198, 2458, 2716, 2993
TaqI TCGA 7 cut(s) 469, 1711, 1926, 2239, 2348, 3240, 3450
TatI WGTACW 5 cut(s) 580, 709, 1117, 1384, 3315
TauI GCSGC 8 cut(s) 40, 134, 152, 413, 545, 1211, 1556, 1624
TfiI GAWTC 7 cut(s) 1606, 1931, 1989, 2236, 2546, 2660, 3406
TscAI CASTG 4 cut(s) 964, 1426, 2162, 2185
TseFI GTSAC 9 cut(s) 353, 360, 492, 890, 909, 1084, 1161, 1714, 3002
TseI GCWGC 5 cut(s) 124, 671, 1727, 2459, 2951
Tsp45I GTSAC 9 cut(s) 353, 360, 492, 890, 909, 1084, 1161, 1714, 3002
TspGWI ACGGA 2 cut(s) 240, 1484
TspRI CASTG 4 cut(s) 964, 1426, 2162, 2185
Van91I CCANNNNNTGG 2 cut(s) 2422, 3142
VpaK11BI GGWCC 2 cut(s) 1425, 2708
VspI ATTAAT 1 cut(s) 3185
XagI CCTNNNNNAGG 1 cut(s) 1296
XapI RAATTY 3 cut(s) 1490, 2393, 3630
XbaI TCTAGA 4 cut(s) 751, 2539, 2884, 3070
XceI RCATGY 1 cut(s) 1802
XcmI CCANNNNNNNNNTGG 1 cut(s) 2811
XhoI CTCGAG 1 cut(s) 468
XmaJI CCTAGG 1 cut(s) 2330
XmiI GTMKAC 4 cut(s) 43, 980, 1109, 2592
XmnI GAANNNNTTC 3 cut(s) 1401, 1605, 2892
XspI CTAG 9 cut(s) 752, 1487, 1815, 2331, 2540, 2588, 2885, 2966, 3071
Zsp2I ATGCAT 3 cut(s) 613, 1800, 2376
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.