Probably involved in the RNA silencing pathway and required for the generation of small interfering RNAs (siRNAs)
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc65I | GGTACC | 1 cut(s) | 598 |
| AccB1I | GGYRCC | 1 cut(s) | 598 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 637 |
| AccI | GTMKAC | 1 cut(s) | 840 |
| AciI | CCGC | 3 cut(s) | 772, 984, 1326 |
| AclI | AACGTT | 1 cut(s) | 410 |
| AclWI | GGATC | 5 cut(s) | 24, 37, 269, 476, 1217 |
| AcoI | YGGCCR | 1 cut(s) | 1323 |
| AcsI | RAATTY | 2 cut(s) | 347, 608 |
| AcuI | CTGAAG | 3 cut(s) | 351, 827, 1146 |
| AcvI | CACGTG | 1 cut(s) | 671 |
| AfaI | GTAC | 6 cut(s) | 14, 538, 600, 834, 1046, 1310 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 26, 637 |
| AflIII | ACRYGT | 1 cut(s) | 668 |
| AgsI | TTSAA | 3 cut(s) | 98, 1070, 1175 |
| AhlI | ACTAGT | 1 cut(s) | 835 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 900, 932 |
| AluBI | AGCT | 7 cut(s) | 66, 206, 388, 430, 635, 1033, 1073 |
| AluI | AGCT | 7 cut(s) | 66, 206, 388, 430, 635, 1033, 1073 |
| Alw26I | GTCTC | 3 cut(s) | 125, 785, 1021 |
| AlwI | GGATC | 5 cut(s) | 24, 37, 269, 476, 1217 |
| AoxI | GGCC | 2 cut(s) | 314, 1323 |
| ApeKI | GCWGC | 2 cut(s) | 674, 1328 |
| ApoI | RAATTY | 2 cut(s) | 347, 608 |
| AseI | ATTAAT | 2 cut(s) | 731, 1257 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1140 |
| Asp718I | GGTACC | 1 cut(s) | 598 |
| AspA2I | CCTAGG | 1 cut(s) | 545 |
| AspS9I | GGNCC | 1 cut(s) | 956 |
| AsuC2I | CCSGG | 2 cut(s) | 34, 852 |
| AsuHPI | GGTGA | 3 cut(s) | 713, 1013, 1232 |
| AvaII | GGWCC | 1 cut(s) | 956 |
| AvrII | CCTAGG | 1 cut(s) | 545 |
| BamHI | GGATCC | 1 cut(s) | 29 |
| BanI | GGYRCC | 1 cut(s) | 598 |
| BbrPI | CACGTG | 1 cut(s) | 671 |
| BbsI | GAAGAC | 1 cut(s) | 1209 |
| BbvCI | CCTCAGC | 1 cut(s) | 1029 |
| BbvI | GCAGC | 2 cut(s) | 686, 1315 |
| BccI | CCATC | 8 cut(s) | 24, 170, 441, 466, 806, 992, 1117, 1315 |
| BciVI | GTATCC | 1 cut(s) | 152 |
| BcnI | CCSGG | 2 cut(s) | 34, 852 |
| BcoDI | GTCTC | 3 cut(s) | 125, 785, 1021 |
| BcuI | ACTAGT | 1 cut(s) | 835 |
| BfaI | CTAG | 4 cut(s) | 546, 836, 1133, 1167 |
| BfmI | CTRYAG | 1 cut(s) | 1403 |
| BfuI | GTATCC | 1 cut(s) | 152 |
| BglII | AGATCT | 2 cut(s) | 1129, 1138 |
| BisI | GCNGC | 3 cut(s) | 675, 1326, 1329 |
| BlnI | CCTAGG | 1 cut(s) | 545 |
| BlsI | GCNGC | 3 cut(s) | 676, 1327, 1330 |
| BmcAI | AGTACT | 1 cut(s) | 538 |
| Bme1390I | CCNGG | 2 cut(s) | 34, 852 |
| Bme18I | GGWCC | 1 cut(s) | 956 |
| BmgT120I | GGNCC | 1 cut(s) | 956 |
| BmiI | GGNNCC | 2 cut(s) | 31, 600 |
| BmrFI | CCNGG | 2 cut(s) | 34, 852 |
| BmrI | ACTGGG | 2 cut(s) | 1021, 1092 |
| BmsI | GCATC | 4 cut(s) | 385, 634, 1201, 1261 |
| BmuI | ACTGGG | 2 cut(s) | 1021, 1092 |
| BoxI | GACNNNNGTC | 1 cut(s) | 820 |
| BpiI | GAAGAC | 1 cut(s) | 1209 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1029 |
| BpuEI | CTTGAG | 1 cut(s) | 1003 |
| BpuMI | CCSGG | 2 cut(s) | 34, 852 |
| Bsa29I | ATCGAT | 2 cut(s) | 105, 708 |
| BsaAI | YACGTR | 1 cut(s) | 671 |
| BsaBI | GATNNNNATC | 3 cut(s) | 190, 1002, 1191 |
| BsaI | GGTCTC | 1 cut(s) | 1021 |
| BsaJI | CCNNGG | 5 cut(s) | 90, 195, 545, 1061, 1178 |
| BsaWI | WCCGGW | 1 cut(s) | 601 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 523, 553 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 26, 637 |
| Bse1I | ACTGG | 5 cut(s) | 187, 342, 522, 1016, 1098 |
| Bse3DI | GCAATG | 1 cut(s) | 966 |
| Bse8I | GATNNNNATC | 3 cut(s) | 190, 1002, 1191 |
| BseCI | ATCGAT | 2 cut(s) | 105, 708 |
| BseDI | CCNNGG | 5 cut(s) | 90, 195, 545, 1061, 1178 |
| BseGI | GGATG | 5 cut(s) | 16, 191, 433, 817, 1192 |
| BseJI | GATNNNNATC | 3 cut(s) | 190, 1002, 1191 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 26, 637 |
| BseMI | GCAATG | 1 cut(s) | 966 |
| BseMII | CTCAG | 3 cut(s) | 681, 798, 1043 |
| BseNI | ACTGG | 5 cut(s) | 187, 342, 522, 1016, 1098 |
| BseRI | GAGGAG | 1 cut(s) | 531 |
| BseXI | GCAGC | 2 cut(s) | 686, 1315 |
| BsgI | GTGCAG | 1 cut(s) | 693 |
| BshFI | GGCC | 2 cut(s) | 316, 1325 |
| BshNI | GGYRCC | 1 cut(s) | 598 |
| BshVI | ATCGAT | 2 cut(s) | 105, 708 |
| BsiSI | CCGG | 3 cut(s) | 33, 602, 851 |
| BslFI | GGGAC | 2 cut(s) | 279, 1250 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 26, 637 |
| BsmAI | GTCTC | 3 cut(s) | 125, 785, 1021 |
| BsmBI | CGTCTC | 1 cut(s) | 785 |
| BsmFI | GGGAC | 2 cut(s) | 279, 1250 |
| BsnI | GGCC | 2 cut(s) | 316, 1325 |
| Bso31I | GGTCTC | 1 cut(s) | 1021 |
| BspACI | CCGC | 3 cut(s) | 772, 984, 1326 |
| BspANI | GGCC | 2 cut(s) | 316, 1325 |
| BspCNI | CTCAG | 3 cut(s) | 682, 797, 1042 |
| BspDI | ATCGAT | 2 cut(s) | 105, 708 |
| BspLI | GGNNCC | 2 cut(s) | 31, 600 |
| BspPI | GGATC | 5 cut(s) | 24, 37, 269, 476, 1217 |
| BspT107I | GGYRCC | 1 cut(s) | 598 |
| BspTNI | GGTCTC | 1 cut(s) | 1021 |
| BsrDI | GCAATG | 1 cut(s) | 966 |
| BsrI | ACTGG | 5 cut(s) | 187, 342, 522, 1016, 1098 |
| BssECI | CCNNGG | 5 cut(s) | 90, 195, 545, 1061, 1178 |
| BssNAI | GTATAC | 1 cut(s) | 841 |
| BssT1I | CCWWGG | 4 cut(s) | 90, 545, 1061, 1178 |
| Bst1107I | GTATAC | 1 cut(s) | 841 |
| Bst4CI | ACNGT | 7 cut(s) | 119, 299, 358, 822, 935, 1208, 1308 |
| BstBAI | YACGTR | 1 cut(s) | 671 |
| BstC8I | GCNNGC | 1 cut(s) | 1276 |
| BstDEI | CTNAG | 4 cut(s) | 323, 690, 784, 1029 |
| BstF5I | GGATG | 5 cut(s) | 16, 191, 433, 817, 1192 |
| BstMAI | GTCTC | 3 cut(s) | 125, 785, 1021 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 820 |
| BstSCI | CCNGG | 2 cut(s) | 32, 850 |
| BstSFI | CTRYAG | 1 cut(s) | 1403 |
| BstV1I | GCAGC | 2 cut(s) | 686, 1315 |
| BstV2I | GAAGAC | 1 cut(s) | 1209 |
| BstX2I | RGATCY | 4 cut(s) | 29, 481, 1129, 1138 |
| BstYI | RGATCY | 4 cut(s) | 29, 481, 1129, 1138 |
| BstZ17I | GTATAC | 1 cut(s) | 841 |
| Bsu15I | ATCGAT | 2 cut(s) | 105, 708 |
| BsuI | GTATCC | 1 cut(s) | 152 |
| BsuRI | GGCC | 2 cut(s) | 316, 1325 |
| BsuTUI | ATCGAT | 2 cut(s) | 105, 708 |
| BtsCI | GGATG | 5 cut(s) | 16, 191, 433, 817, 1192 |
| BtsIMutI | CAGTG | 1 cut(s) | 515 |
| Cac8I | GCNNGC | 1 cut(s) | 1276 |
| Cfr13I | GGNCC | 1 cut(s) | 956 |
| ClaI | ATCGAT | 2 cut(s) | 105, 708 |
| Csp6I | GTAC | 6 cut(s) | 13, 537, 599, 833, 1045, 1309 |
| CviAII | CATG | 5 cut(s) | 259, 590, 616, 650, 875 |
| CviQI | GTAC | 6 cut(s) | 13, 537, 599, 833, 1045, 1309 |
| DdeI | CTNAG | 4 cut(s) | 323, 690, 784, 1029 |
| EaeI | YGGCCR | 1 cut(s) | 1323 |
| Eco130I | CCWWGG | 4 cut(s) | 90, 545, 1061, 1178 |
| Eco31I | GGTCTC | 1 cut(s) | 1021 |
| Eco32I | GATATC | 1 cut(s) | 848 |
| Eco47I | GGWCC | 1 cut(s) | 956 |
| Eco57I | CTGAAG | 3 cut(s) | 351, 827, 1146 |
| Eco72I | CACGTG | 1 cut(s) | 671 |
| EcoRV | GATATC | 1 cut(s) | 848 |
| EcoT14I | CCWWGG | 4 cut(s) | 90, 545, 1061, 1178 |
| EcoT22I | ATGCAT | 1 cut(s) | 591 |
| ErhI | CCWWGG | 4 cut(s) | 90, 545, 1061, 1178 |
| Esp3I | CGTCTC | 1 cut(s) | 785 |
| FaeI | CATG | 5 cut(s) | 262, 593, 619, 653, 878 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1045, 1077 |
| FaqI | GGGAC | 2 cut(s) | 279, 1250 |
| FatI | CATG | 5 cut(s) | 258, 589, 615, 649, 874 |
| FblI | GTMKAC | 1 cut(s) | 840 |
| Fnu4HI | GCNGC | 3 cut(s) | 675, 1326, 1329 |
| FokI | GGATG | 5 cut(s) | 3, 178, 420, 824, 1199 |
| Fsp4HI | GCNGC | 3 cut(s) | 675, 1326, 1329 |
| FspBI | CTAG | 4 cut(s) | 546, 836, 1133, 1167 |
| GluI | GCNGC | 3 cut(s) | 675, 1326, 1329 |
| HaeIII | GGCC | 2 cut(s) | 316, 1325 |
| HapII | CCGG | 3 cut(s) | 33, 602, 851 |
| Hin1II | CATG | 5 cut(s) | 262, 593, 619, 653, 878 |
| HindIII | AAGCTT | 2 cut(s) | 633, 1071 |
| HinfI | GANTC | 8 cut(s) | 4, 128, 186, 451, 761, 908, 1105, 1399 |
| HpaII | CCGG | 3 cut(s) | 33, 602, 851 |
| HphI | GGTGA | 3 cut(s) | 713, 1013, 1232 |
| Hpy166II | GTNNAC | 4 cut(s) | 841, 857, 959, 1058 |
| Hpy188I | TCNGA | 4 cut(s) | 196, 438, 787, 1114 |
| Hpy8I | GTNNAC | 4 cut(s) | 841, 857, 959, 1058 |
| HpyAV | CCTTC | 4 cut(s) | 256, 802, 823, 892 |
| HpyCH4III | ACNGT | 7 cut(s) | 119, 299, 358, 822, 935, 1208, 1308 |
| HpyCH4IV | ACGT | 3 cut(s) | 410, 670, 961 |
| HpyCH4V | TGCA | 7 cut(s) | 345, 578, 589, 619, 663, 674, 951 |
| HpyF3I | CTNAG | 4 cut(s) | 323, 690, 784, 1029 |
| HpySE526I | ACGT | 3 cut(s) | 410, 670, 961 |
| Hsp92II | CATG | 5 cut(s) | 262, 593, 619, 653, 878 |
| KpnI | GGTACC | 1 cut(s) | 602 |
| LmnI | GCTCC | 1 cut(s) | 435 |
| Lsp1109I | GCAGC | 2 cut(s) | 686, 1315 |
| LweI | GCATC | 4 cut(s) | 385, 634, 1201, 1261 |
| MaeI | CTAG | 4 cut(s) | 546, 836, 1133, 1167 |
| MaeII | ACGT | 3 cut(s) | 410, 670, 961 |
| MboII | GAAGA | 9 cut(s) | 122, 344, 617, 653, 934, 1133, 1139, 1148, 1214 |
| MflI | RGATCY | 4 cut(s) | 29, 481, 1129, 1138 |
| MluCI | AATT | 7 cut(s) | 210, 318, 347, 573, 608, 715, 728 |
| MlyI | GAGTC | 1 cut(s) | 1114 |
| MmeI | TCCRAC | 2 cut(s) | 509, 898 |
| MnlI | CCTC | 7 cut(s) | 190, 552, 558, 587, 762, 1038, 1285 |
| Mph1103I | ATGCAT | 1 cut(s) | 591 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1140 |
| MseI | TTAA | 7 cut(s) | 68, 209, 474, 572, 627, 731, 1257 |
| MslI | CAYNNNNRTG | 2 cut(s) | 150, 174 |
| MspA1I | CMGCKG | 1 cut(s) | 1328 |
| MspI | CCGG | 3 cut(s) | 33, 602, 851 |
| MspR9I | CCNGG | 2 cut(s) | 34, 852 |
| NciI | CCSGG | 2 cut(s) | 34, 852 |
| NlaIII | CATG | 5 cut(s) | 262, 593, 619, 653, 878 |
| NlaIV | GGNNCC | 2 cut(s) | 31, 600 |
| NsiI | ATGCAT | 1 cut(s) | 591 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 560 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1140 |
| PfeI | GAWTC | 7 cut(s) | 4, 128, 186, 451, 761, 908, 1399 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 637 |
| PkrI | GCNGC | 3 cut(s) | 676, 1327, 1330 |
| PleI | GAGTC | 1 cut(s) | 1113 |
| PmaCI | CACGTG | 1 cut(s) | 671 |
| PmlI | CACGTG | 1 cut(s) | 671 |
| PpsI | GAGTC | 1 cut(s) | 1113 |
| Ppu21I | YACGTR | 1 cut(s) | 671 |
| PshAI | GACNNNNGTC | 1 cut(s) | 820 |
| PshBI | ATTAAT | 2 cut(s) | 731, 1257 |
| Psp1406I | AACGTT | 1 cut(s) | 410 |
| PspCI | CACGTG | 1 cut(s) | 671 |
| PspN4I | GGNNCC | 2 cut(s) | 31, 600 |
| PspPI | GGNCC | 1 cut(s) | 956 |
| PsuI | RGATCY | 4 cut(s) | 29, 481, 1129, 1138 |
| RsaI | GTAC | 6 cut(s) | 14, 538, 600, 834, 1046, 1310 |
| RsaNI | GTAC | 6 cut(s) | 13, 537, 599, 833, 1045, 1309 |
| RseI | CAYNNNNRTG | 2 cut(s) | 150, 174 |
| SaqAI | TTAA | 7 cut(s) | 68, 209, 474, 572, 627, 731, 1257 |
| SatI | GCNGC | 3 cut(s) | 675, 1326, 1329 |
| Sau96I | GGNCC | 1 cut(s) | 956 |
| ScaI | AGTACT | 1 cut(s) | 538 |
| SchI | GAGTC | 1 cut(s) | 1114 |
| ScrFI | CCNGG | 2 cut(s) | 34, 852 |
| SfaNI | GCATC | 4 cut(s) | 385, 634, 1201, 1261 |
| SfcI | CTRYAG | 1 cut(s) | 1403 |
| SinI | GGWCC | 1 cut(s) | 956 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 150, 174 |
| SmlI | CTYRAG | 1 cut(s) | 1018 |
| SmoI | CTYRAG | 1 cut(s) | 1018 |
| SpeI | ACTAGT | 1 cut(s) | 835 |
| Sse9I | AATT | 7 cut(s) | 210, 318, 347, 573, 608, 715, 728 |
| SsiI | CCGC | 3 cut(s) | 772, 984, 1326 |
| SspMI | CTAG | 4 cut(s) | 546, 836, 1133, 1167 |
| StyD4I | CCNGG | 2 cut(s) | 32, 850 |
| StyI | CCWWGG | 4 cut(s) | 90, 545, 1061, 1178 |
| TaaI | ACNGT | 7 cut(s) | 119, 299, 358, 822, 935, 1208, 1308 |
| TaiI | ACGT | 3 cut(s) | 413, 673, 964 |
| TaqI | TCGA | 6 cut(s) | 105, 123, 454, 563, 708, 1233 |
| TasI | AATT | 7 cut(s) | 210, 318, 347, 573, 608, 715, 728 |
| TatI | WGTACW | 2 cut(s) | 536, 1308 |
| TauI | GCSGC | 1 cut(s) | 1328 |
| TfiI | GAWTC | 7 cut(s) | 4, 128, 186, 451, 761, 908, 1399 |
| Tru1I | TTAA | 7 cut(s) | 68, 209, 474, 572, 627, 731, 1257 |
| Tru9I | TTAA | 7 cut(s) | 68, 209, 474, 572, 627, 731, 1257 |
| TscAI | CASTG | 1 cut(s) | 522 |
| TseI | GCWGC | 2 cut(s) | 674, 1328 |
| TspRI | CASTG | 1 cut(s) | 522 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 637 |
| VpaK11BI | GGWCC | 1 cut(s) | 956 |
| VspI | ATTAAT | 2 cut(s) | 731, 1257 |
| XapI | RAATTY | 2 cut(s) | 347, 608 |
| XbaI | TCTAGA | 1 cut(s) | 1132 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1059 |
| XmaJI | CCTAGG | 1 cut(s) | 545 |
| XmiI | GTMKAC | 1 cut(s) | 840 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1140 |
| XspI | CTAG | 4 cut(s) | 546, 836, 1133, 1167 |
| ZrmI | AGTACT | 1 cut(s) | 538 |
| Zsp2I | ATGCAT | 1 cut(s) | 591 |