MD15G1195300.v1.1
ERF Family

Probably involved in the RNA silencing pathway and required for the generation of small interfering RNAs (siRNAs)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0000505
Physical Location & Seq
Forward (+)
15458928 .. 15460533
1606 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1195300.v1.1.491

Sequence Viewer

Length: 369 bp
ATGGATGAGTATCACTCTCAAGGCTGGTTTATCTTTAAATTAGTGAATTACCATAATCTTTGCTGGTTCACTTTGGATTGGGATTCTCGACAAGCATACGTCTACCATTGTGAAGTCGCTGTGGATGGGAGCTACAAATTCAAGGGCCCCTATCAACACACCACAAAAAGAACTCTCTTACAGAAAATTTTAGGAGATGACAGCACTTTAATGGTGAAATTTGCTGATGCGGTGAATGACAAAGATCCCTGGAAGAAAATGGGCCTTTATGTTAATTATAGCAAGGTCGCAAGAGAAGGGATTCTTGTTGGTTTGCGCCGATATTGTTTCTTTGGTATGCCTTTAATACCTTCTTATGGTAAATTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RdRP

Protein Analysis

123

Amino Acids

14.41

Weight (kDa)

8.93

Isoelectric Point (pI)

35.15

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000505)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G19910 AT2G19910 AT2G19920 AT2G19920 AT2G19920 AT2G19920 AT2G19930 AT2G19930 AT2G19930
fragaria_vesca FvH4_1g05980 FvH4_1g05990 FvH4_1g05990 FvH4_1g09718
malus_domestica MD02G1064400.v1.1 MD15G1195300.v1.1 MD15G1195400.v1.1
prunus_persica Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1
pyrus_communis pycom02g05070 pycom15g17460
rosa_chinensis RchiOBHm_Chr2g0091671 RchiOBHm_Chr2g0091681 RchiOBHm_Chr2g0091691 RchiOBHm_Chr2g0091701 RchiOBHm_Chr2g0091711 RchiOBHm_Chr2g0091721 RchiOBHm_Chr2g0091731 RchiOBHm_Chr5g0014401
rosa_laevigata RLG00000013772 RLG00000016242 RLG00000016244 RLG00000016245 RLG00000016246 RLG00000032105
rosa_multiflora Rmu_co8374041.1_g000001 Rmu_co8478121.1_g000001 Rmu_sc0000897.1_g000005 Rmu_sc0000897.1_g000009
rosa_roxburghii Rroxscaffold_1G00062180 Rroxscaffold_1G00062190 Rroxscaffold_1G00068800 Rroxscaffold_2G00149790 Rroxscaffold_2G00149800 Rroxscaffold_2G00149810
rosa_rugosa Rorug02G0019600 Rorug02G0019700 Rorug02G0019800 Rorug02G0019900 Rorug02G0019900 Rorug02G0020000 Rorug02G0020000 Rorug02G0020100 Rorug02G0020100 Rorug02G0020200 Rorug05G0017800
rosa_samantha Rh2AG064700 Rh2AG064800 Rh2AG064900 Rh2BG063800 Rh2BG063900 Rh2BG064000 Rh2BG064100 Rh2CG065500 Rh2CG065600 Rh2CG065700 Rh2DG063900 Rh2DG064000 Rh2DG064100 Rh2DG064200 Rh2DG381700 Rh5AG056700 Rh5AG056800 Rh5AG056900 Rh5AG112600 Rh5BG109300 Rh5CG063800 Rh5CG121100 Rh5DG053100 Rh5DG108300 Rh6CG100100
rosa_wichuraiana Rw2G005560 Rw2G005580 Rw2G005590 Rw5G005370 Rw5G009770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 102
AciI CCGC 1 cut(s) 230
AclWI GGATC 1 cut(s) 239
AcsI RAATTY 4 cut(s) 137, 186, 218, 362
AfiI CCNNNNNNNGG 1 cut(s) 356
AgsI TTSAA 1 cut(s) 142
AjnI CCWGG 1 cut(s) 248
AluBI AGCT 1 cut(s) 132
AluI AGCT 1 cut(s) 132
AlwI GGATC 1 cut(s) 239
AoxI GGCC 2 cut(s) 145, 262
ApaI GGGCCC 1 cut(s) 149
ApoI RAATTY 4 cut(s) 137, 186, 218, 362
Asp700I GAANNNNTTC 1 cut(s) 300
AspLEI GCGC 1 cut(s) 318
AspS9I GGNCC 3 cut(s) 145, 146, 262
AsuHPI GGTGA 2 cut(s) 226, 244
BaeGI GKGCMC 1 cut(s) 149
BanII GRGCYC 1 cut(s) 149
BccI CCATC 1 cut(s) 119
BciT130I CCWGG 1 cut(s) 250
Bme1390I CCNGG 1 cut(s) 250
BmgT120I GGNCC 3 cut(s) 145, 146, 262
BmiI GGNNCC 2 cut(s) 147, 148
BmrFI CCNGG 1 cut(s) 250
BmsI GCATC 1 cut(s) 217
BplI GAGNNNNNCTC 1 cut(s) 31
BsaBI GATNNNNATC 1 cut(s) 9
BsaJI CCNNGG 1 cut(s) 248
Bsc4I CCNNNNNNNGG 1 cut(s) 356
Bse8I GATNNNNATC 1 cut(s) 9
BseBI CCWGG 1 cut(s) 250
BseDI CCNNGG 1 cut(s) 248
BseGI GGATG 2 cut(s) 10, 130
BseJI GATNNNNATC 1 cut(s) 9
BseLI CCNNNNNNNGG 1 cut(s) 356
BseSI GKGCMC 1 cut(s) 149
BshFI GGCC 2 cut(s) 147, 264
BslI CCNNNNNNNGG 1 cut(s) 356
BsnI GGCC 2 cut(s) 147, 264
Bsp120I GGGCCC 1 cut(s) 145
Bsp1286I GDGCHC 1 cut(s) 149
Bsp143I GATC 1 cut(s) 244
BspACI CCGC 1 cut(s) 230
BspANI GGCC 2 cut(s) 147, 264
BspLI GGNNCC 2 cut(s) 147, 148
BspPI GGATC 1 cut(s) 239
BssECI CCNNGG 1 cut(s) 248
BssMI GATC 1 cut(s) 244
Bst2UI CCWGG 1 cut(s) 250
BstF5I GGATG 2 cut(s) 10, 130
BstHHI GCGC 1 cut(s) 318
BstKTI GATC 1 cut(s) 247
BstMBI GATC 1 cut(s) 244
BstNI CCWGG 1 cut(s) 250
BstSCI CCNGG 1 cut(s) 248
BstSLI GKGCMC 1 cut(s) 149
BstX2I RGATCY 1 cut(s) 244
BstYI RGATCY 1 cut(s) 244
BsuRI GGCC 2 cut(s) 147, 264
BtsCI GGATG 2 cut(s) 10, 130
CfoI GCGC 1 cut(s) 318
Cfr13I GGNCC 3 cut(s) 145, 146, 262
CviJI RGCY 4 cut(s) 24, 132, 147, 264
CviKI_1 RGCY 4 cut(s) 24, 132, 147, 264
DpnI GATC 1 cut(s) 246
DpnII GATC 1 cut(s) 244
DraI TTTAAA 1 cut(s) 37
Eco24I GRGCYC 1 cut(s) 149
EcoO109I RGGNCCY 2 cut(s) 145, 146
EcoRII CCWGG 1 cut(s) 248
EcoT38I GRGCYC 1 cut(s) 149
FaiI YATR 6 cut(s) 54, 97, 270, 279, 338, 357
FalI AAGNNNNNCTT 2 cut(s) 288, 320
FblI GTMKAC 1 cut(s) 102
FokI GGATG 2 cut(s) 17, 137
FriOI GRGCYC 1 cut(s) 149
GlaI GCGC 1 cut(s) 317
HaeIII GGCC 2 cut(s) 147, 264
HhaI GCGC 1 cut(s) 318
Hin6I GCGC 1 cut(s) 316
HinP1I GCGC 1 cut(s) 316
HinfI GANTC 2 cut(s) 83, 301
HphI GGTGA 2 cut(s) 226, 244
Hpy166II GTNNAC 2 cut(s) 69, 103
Hpy188III TCNNGA 1 cut(s) 87
Hpy8I GTNNAC 2 cut(s) 69, 103
HpyAV CCTTC 2 cut(s) 290, 360
HpyCH4IV ACGT 1 cut(s) 99
HpySE526I ACGT 1 cut(s) 99
HspAI GCGC 1 cut(s) 316
Kzo9I GATC 1 cut(s) 244
LmnI GCTCC 1 cut(s) 129
LpnPI CCDG 4 cut(s) 10, 49, 235, 262
LweI GCATC 1 cut(s) 217
MaeII ACGT 1 cut(s) 99
MalI GATC 1 cut(s) 246
MboI GATC 1 cut(s) 244
MboII GAAGA 1 cut(s) 265
MflI RGATCY 1 cut(s) 244
MhlI GDGCHC 1 cut(s) 149
MluCI AATT 7 cut(s) 38, 46, 137, 186, 218, 274, 362
MroXI GAANNNNTTC 1 cut(s) 300
MseI TTAA 4 cut(s) 36, 209, 273, 344
MslI CAYNNNNRTG 1 cut(s) 209
MspR9I CCNGG 1 cut(s) 250
MvaI CCWGG 1 cut(s) 250
NdeII GATC 1 cut(s) 244
NlaIV GGNNCC 2 cut(s) 147, 148
PdmI GAANNNNTTC 1 cut(s) 300
PfeI GAWTC 2 cut(s) 83, 301
Psp6I CCWGG 1 cut(s) 248
PspGI CCWGG 1 cut(s) 248
PspN4I GGNNCC 2 cut(s) 147, 148
PspOMI GGGCCC 1 cut(s) 145
PspPI GGNCC 3 cut(s) 145, 146, 262
PsuI RGATCY 1 cut(s) 244
RseI CAYNNNNRTG 1 cut(s) 209
SaqAI TTAA 4 cut(s) 36, 209, 273, 344
Sau3AI GATC 1 cut(s) 244
Sau96I GGNCC 3 cut(s) 145, 146, 262
ScrFI CCNGG 1 cut(s) 250
SduI GDGCHC 1 cut(s) 149
SetI ASST 4 cut(s) 102, 134, 288, 352
SfaNI GCATC 1 cut(s) 217
SmiMI CAYNNNNRTG 1 cut(s) 209
SmlI CTYRAG 1 cut(s) 18
SmoI CTYRAG 1 cut(s) 18
Sse9I AATT 7 cut(s) 38, 46, 137, 186, 218, 274, 362
SsiI CCGC 1 cut(s) 230
StyD4I CCNGG 1 cut(s) 248
TaiI ACGT 1 cut(s) 102
TaqI TCGA 1 cut(s) 88
TasI AATT 7 cut(s) 38, 46, 137, 186, 218, 274, 362
TfiI GAWTC 2 cut(s) 83, 301
Tru1I TTAA 4 cut(s) 36, 209, 273, 344
Tru9I TTAA 4 cut(s) 36, 209, 273, 344
XapI RAATTY 4 cut(s) 137, 186, 218, 362
XmiI GTMKAC 1 cut(s) 102
XmnI GAANNNNTTC 1 cut(s) 300
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.