Rh5BG109300
ERF Family

Probably involved in the RNA silencing pathway and required for the generation of small interfering RNAs (siRNAs)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0000505
Physical Location & Seq
Reverse (-)
10220920 .. 10231127
10208 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG109300.1

Sequence Viewer

Length: 3306 bp
ATGTTCGGCTTCCAGCTACTCGACTCCGTGCAGCAATCGCTGGATCGAATATGCCAACTTAAGAAGCAACCGCAGCCGGACGACGGCATCAGAAGAAAGCTGGCATTGCTGAGCGAGCAAGACGCCCTCTACGTGCTGGACCAAATTTACAACAGCAAAACTGAGATCAGAACCCTCAATGGATTCATTGCTTTCATGATCAGGAAACTAGAGAATGAATCTTCTTCGTCTCCTTCTTCTAGAACAGTTTGGACCCCTCAGAGTCCCTCTGCTTCTTCTTCTTCTGTTACTAGTCCTGCACAGCTTTATCACAGCCCTGATCATTCTCAAGTTGTAGACCAGTCTGAAGAGAGTGGACTCTGTTCCTCTGTTTTTCAGCGGCTATATTTGAGCTCTCAAGGTGAGACAATTCCTTGTGGTTCTCCTGGTGTAGAGCAACATCAAGACTATAATCGACAGCCACTCTATTCTTCAGCTTCTCCTTCTGAAGCTTTCCAGACTTCTTATTATCAGAGCCATTCTTCTCCTACTAGTACTGATGCACCATTTTATTCCAGTTCTCAGCCTCAGGGTGAGACTCCCAACCCATCCCCGATGACAGTGTGCAATGGCCAATCACCTTATTCCTCCCCTTCTCCTTATCAAATCCTGTCTGCTCCTTCTAATACTCATAGACAACTCTATGAGAGGTTCCAAGGTGAGACTGTCACTCACTCCCCAGTTCTATACCATCCTGGTGACCATGGACCAGCACACTTAGAAGCTCTGGGAGAACTCGAGTTCAGAAGACAATTTCTAATATTAAGTTATGCTGGAGGAACGAAGTTAAAAGATGTAATAGAAGTGGAGGCTATTAGGCGCTGGAAAAATTTGCCAATGGTAAACTTCGAGACCAAAGTTTGGCATGCTTTGGGTGAAAACAATGTTGGCAAGGCTGACCGACGATTGGGTCCCTTCCTAGACAAACCAAGAACTCTCCTACAGAAATGGTTAGACGATGACAATGTTTTAGAGGTGAAATTTACCCAAGGGGAAGGTTCCAGTAGTCTTTTTGATAATAATTATGGTACATATAGCAAGTTAATGCGAGAAGGCATTCTTGTTGGTTTGCGGCGATATGGTCACTTTGTGTACAGTGTTGGTGGTAACAAAGGAAAGAATAAGTTGAAAGACCCAACTTCATCACCTGTGAAATGTTACTTTGTTCGTATGAGCTCTGATGCAGCGATAGACATGGGTAAATTCTATAAGTTAGCTGGCAAAACGATGCATGAAGGTAGATCTCTTTTGACGCACGTACACACTGGGCCTAGTTTGGCTAATTACATGGCTAGGTTATCTCTCATTTTGTCCAAGACAGTTACTTTAGAAGTGAATTGGTCACTTGTCAAGGTTGAGTTCATCAATGAAGAATACTGTCTCGATGAGTCTGGTAATCCCATCTATGTAGATGGAAAAGCTCGTATACATACTGATGGCACGGGATTTATATCTGAGGATCTGGCTTTACTTTGTCCTGAGATAGCACACAAAGGAGAGCTTATCAATAATGAACACATCGAGGGACTTGATGATCCTGATGATGAACTTGAGGACAACATTCTGGAATGGAAACAGCCAGGCGCTAGAACTCAGGAACTGCCTTTGCTCATGCAGTTCCGACTTTTTTACAAAGGCTATGCTATCAAAGGAACTTTTCTTGTCAATAAACTGCTTCCACCAAACACATTGCAGATTCGACCGTCAATGGTCAAAGTTGAGCCAGATCCAAAATTGTCAGATCCTCAAACAGTGAATTCACTGGAGATAGTAGGAACAAGTAAACTTACTAAGAGAACAACTTTGTCGAGGAACTTGATTGCACTTTTATACCATGGAGGGGTGCCAAAAGAATACTTCATCGAGTTACTTATGAAAGCTTTAGAAGAGACTCGTGATGTTTATAGCAATAAACGTGCAGCATTCAGAGTTGCCCTTAGATATGGCGAGATCGACGACAATAGTAATGTTGCGAAAATGATCTCCTGTGGTATTCCTCTTGAAGAACCATATGTGCAATATCGTCTTTCAGCCCTTATGAACATGGAAAAGATCAATCTTAAAAAAGGGAAAATTTGTATTCCTGAATCATACTATTTGATGGGGACGGCTGATCCAACGGGAAAACTTGAAAAAGATGAAGTTTGCATTATCCTAAAAGATGGACAACTGTCGGGAAAGGTTCTAGTGTACCGGAATCCTGGTGTACACTTCGGTGATATTCATGTCCTAAAAGCCACGTATGTCAAGGAACTAGAGTCTGTTGTTGGAAATGCCAAGTACGGTATATTCTTCTCTTGTAAAGGTCCACGTTCTGTTGCGGATGAAATGGGTGGTGGAGATTTTGATGGTGATCTATACTGGATCTCAAGAAACCCTCAGCTGTTGGAATTGTTTAAACCAAGTGAACCTTGGATTGAAAAGTCATCTACCCAAAAAGTTCATGGTAAAAGACCAACCGAGCTTTCAAATGTTGATCTAGAAGATGAGCTCATCAAACTATTCTTGAAGACTAGGTTTGAACCAAGCTTCGCAATGAGTGAGGCAGCTCAACACTGGATGGTTTGGATGGATAAATTTTTGAGTTTAGGAGATACGAGTATTGATGAGAAAAACGAACTGAAGGCAAAGATACTTCAATTGATTGATATTTACTATGAAGCTCTCGACGCACCCAAAAAAGGTTTTAAGGTTGTAGTTCCAAAAGAATTGAAGGTGAAAAAGTTCCCACATTTCATGGAGAAGAAACCCCTAGATTCCTACCATTCAATTTCCATTTTGGGATCAATTTATGATAAGGTGGATGAATATGAAACAGAAGACAGATCTCTCAAAGAAATTCAGAAACTTCCTCTTTTTGAAGTTGACGTTCCTGAGGGTAGCTTGAAAGAATGGAAGAAACTATACCAAGATTACCGGAATGAGATGACCTCTGCCATGGGAGCCGAAGATAAACAGGGCCAGAATCAGGCAGCCAATATTGTCATAAGAAAGTATAAAGAGATCCTATACAAGGCTGAGGAGTTTGAGCAAAGTGATATGCGGCACAGACAACAAATATATAATGGCGCACTCGCTATTTATCACGTTTGTTATGACTATGCCAAGAGTGTTAGGACTGAAACTGGTGCACAAGATGTCAAGAAATGTGGCTTTGCTTGGAAAGTTGCAGGTGCAGCTCTATGCCAGCTTCACGAAGACTGGAGTTTGAAACCTGGTGAAAAGGTTTTCAGAGTCTTACCCTCTGTTTTGAAGGACATTATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RdRP

Protein Analysis

1101

Amino Acids

124.55

Weight (kDa)

6.19

Isoelectric Point (pI)

45.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
4HB_RdRP3_N PF26249 6 - 69 2.3e-07 RdRP3/4/5, N-terminal four helical bundle domain
RdRP_helical PF26252 252 - 316 3.4e-07 RNA dependent RNA polymerase, helical domain
RdRP PF05183 317 - 947 8e-100 RNA dependent RNA polymerase
RdRP_head PF26253 975 - 1079 4.2e-09 RdRP, head domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000505)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G19910 AT2G19910 AT2G19920 AT2G19920 AT2G19920 AT2G19920 AT2G19930 AT2G19930 AT2G19930
fragaria_vesca FvH4_1g05980 FvH4_1g05990 FvH4_1g05990 FvH4_1g09718
malus_domestica MD02G1064400.v1.1 MD15G1195300.v1.1 MD15G1195400.v1.1
prunus_persica Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1
pyrus_communis pycom02g05070 pycom15g17460
rosa_chinensis RchiOBHm_Chr2g0091671 RchiOBHm_Chr2g0091681 RchiOBHm_Chr2g0091691 RchiOBHm_Chr2g0091701 RchiOBHm_Chr2g0091711 RchiOBHm_Chr2g0091721 RchiOBHm_Chr2g0091731 RchiOBHm_Chr5g0014401
rosa_laevigata RLG00000013772 RLG00000016242 RLG00000016244 RLG00000016245 RLG00000016246 RLG00000032105
rosa_multiflora Rmu_co8374041.1_g000001 Rmu_co8478121.1_g000001 Rmu_sc0000897.1_g000005 Rmu_sc0000897.1_g000009
rosa_roxburghii Rroxscaffold_1G00062180 Rroxscaffold_1G00062190 Rroxscaffold_1G00068800 Rroxscaffold_2G00149790 Rroxscaffold_2G00149800 Rroxscaffold_2G00149810
rosa_rugosa Rorug02G0019600 Rorug02G0019700 Rorug02G0019800 Rorug02G0019900 Rorug02G0019900 Rorug02G0020000 Rorug02G0020000 Rorug02G0020100 Rorug02G0020100 Rorug02G0020200 Rorug05G0017800
rosa_samantha Rh2AG064700 Rh2AG064800 Rh2AG064900 Rh2BG063800 Rh2BG063900 Rh2BG064000 Rh2BG064100 Rh2CG065500 Rh2CG065600 Rh2CG065700 Rh2DG063900 Rh2DG064000 Rh2DG064100 Rh2DG064200 Rh2DG381700 Rh5AG056700 Rh5AG056800 Rh5AG056900 Rh5AG112600 Rh5BG109300 Rh5CG063800 Rh5CG121100 Rh5DG053100 Rh5DG108300 Rh6CG100100
rosa_wichuraiana Rw2G005560 Rw2G005580 Rw2G005590 Rw5G005370 Rw5G009770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 3200
AasI GACNNNNNNGTC 1 cut(s) 948
Acc36I ACCTGC 1 cut(s) 3200
AccB1I GGYRCC 1 cut(s) 1882
AccB7I CCANNNNNTGG 1 cut(s) 900
AccI GTMKAC 2 cut(s) 336, 1465
AciI CCGC 5 cut(s) 71, 379, 1111, 2360, 3082
AclWI GGATC 9 cut(s) 51, 1506, 1568, 1760, 1775, 2147, 2411, 2830, 3037
AcoI YGGCCR 1 cut(s) 610
AcsI RAATTY 8 cut(s) 144, 868, 1019, 1241, 1795, 2112, 2615, 2877
AcuI CTGAAG 4 cut(s) 366, 456, 507, 2681
AcyI GRCGYC 1 cut(s) 123
AdeI CACNNNGTG 1 cut(s) 1129
AfaI GTAC 7 cut(s) 535, 1069, 1133, 1299, 2231, 2247, 2321
AfiI CCNNNNNNNGG 7 cut(s) 83, 900, 946, 1879, 2720, 3007, 3052
AflII CTTAAG 1 cut(s) 59
AhlI ACTAGT 2 cut(s) 290, 530
AjnI CCWGG 5 cut(s) 424, 733, 1618, 2239, 3253
AjuI GAANNNNNNNTTGG 4 cut(s) 909, 941, 2488, 2520
AleI CACNNNNGTG 1 cut(s) 2253
Alw21I GWGCWC 4 cut(s) 395, 1217, 2532, 3172
Alw26I GTCTC 7 cut(s) 234, 398, 569, 695, 884, 1424, 1922
Alw44I GTGCAC 1 cut(s) 3168
AlwI GGATC 9 cut(s) 51, 1506, 1568, 1760, 1775, 2147, 2411, 2830, 3037
AlwNI CAGNNNCTG 1 cut(s) 1639
Ama87I CYCGRG 1 cut(s) 776
AoxI GGCC 3 cut(s) 610, 1307, 2998
ApaLI GTGCAC 1 cut(s) 3168
ApeKI GCWGC 7 cut(s) 31, 73, 1223, 1958, 2585, 3011, 3215
ApoI RAATTY 8 cut(s) 144, 868, 1019, 1241, 1795, 2112, 2615, 2877
Asp700I GAANNNNTTC 2 cut(s) 1095, 2762
AspLEI GCGC 3 cut(s) 861, 1625, 3110
AspS9I GGNCC 7 cut(s) 139, 252, 746, 950, 1307, 2345, 2998
AvaI CYCGRG 1 cut(s) 776
AvaII GGWCC 5 cut(s) 139, 252, 746, 950, 2345
AxyI CCTNAGG 2 cut(s) 567, 2913
BaeGI GKGCMC 1 cut(s) 3172
BalI TGGCCA 1 cut(s) 612
BanI GGYRCC 1 cut(s) 1882
BanII GRGCYC 3 cut(s) 395, 1217, 2532
BarI GAAGNNNNNNTAC 2 cut(s) 2927, 2959
BauI CACGAG 1 cut(s) 1932
BbsI GAAGAC 4 cut(s) 793, 2555, 2865, 3243
Bbv12I GWGCWC 4 cut(s) 395, 1217, 2532, 3172
BbvCI CCTCAGC 2 cut(s) 2418, 3057
BbvI GCAGC 7 cut(s) 43, 85, 1235, 1970, 2597, 3023, 3227
BceAI ACGGC 2 cut(s) 100, 2163
BciT130I CCWGG 5 cut(s) 426, 735, 1620, 2241, 3255
BclI TGATCA 2 cut(s) 198, 319
BcoDI GTCTC 7 cut(s) 234, 398, 569, 695, 884, 1424, 1922
BcuI ACTAGT 2 cut(s) 290, 530
BfmI CTRYAG 1 cut(s) 980
BfoI RGCGCY 2 cut(s) 862, 1626
BfrI CTTAAG 1 cut(s) 59
BfuAI ACCTGC 1 cut(s) 3200
BglII AGATCT 2 cut(s) 1280, 2864
BlpI GCTNAGC 1 cut(s) 110
BmcAI AGTACT 1 cut(s) 535
Bme1390I CCNGG 5 cut(s) 426, 735, 1620, 2241, 3255
Bme18I GGWCC 5 cut(s) 139, 252, 746, 950, 2345
BmeT110I CYCGRG 1 cut(s) 776
BmgT120I GGNCC 7 cut(s) 139, 252, 746, 950, 1307, 2345, 2998
BmiI GGNNCC 7 cut(s) 254, 692, 951, 952, 1039, 1884, 2983
BmrFI CCNGG 5 cut(s) 426, 735, 1620, 2241, 3255
BmrI ACTGGG 2 cut(s) 713, 1314
BmsI GCATC 4 cut(s) 96, 529, 1210, 1257
BmuI ACTGGG 2 cut(s) 713, 1314
BoxI GACNNNNGTC 1 cut(s) 2209
BpiI GAAGAC 4 cut(s) 793, 2555, 2865, 3243
BplI GAGNNNNNCTC 2 cut(s) 2954, 2986
BpmI CTGGAG 3 cut(s) 834, 1823, 3262
Bpu10I CCTNAGC 2 cut(s) 2418, 3057
Bpu1102I GCTNAGC 1 cut(s) 110
BpuEI CTTGAG 4 cut(s) 312, 381, 1610, 2392
BsaAI YACGTR 3 cut(s) 133, 1297, 2280
BsaBI GATNNNNATC 2 cut(s) 1489, 2391
BsaHI GRCGYC 1 cut(s) 123
BsaI GGTCTC 1 cut(s) 884
BsaJI CCNNGG 6 cut(s) 694, 742, 1027, 1873, 2450, 2976
BsaWI WCCGGW 2 cut(s) 2232, 2955
Bsc4I CCNNNNNNNGG 7 cut(s) 83, 900, 946, 1879, 2720, 3007, 3052
Bse21I CCTNAGG 2 cut(s) 567, 2913
Bse3DI GCAATG 5 cut(s) 104, 186, 613, 1727, 2580
Bse8I GATNNNNATC 2 cut(s) 1489, 2391
BseBI CCWGG 5 cut(s) 426, 735, 1620, 2241, 3255
BseDI CCNNGG 6 cut(s) 694, 742, 1027, 1873, 2450, 2976
BseGI GGATG 6 cut(s) 587, 730, 2368, 2604, 2613, 2848
BseJI GATNNNNATC 2 cut(s) 1489, 2391
BseLI CCNNNNNNNGG 7 cut(s) 83, 900, 946, 1879, 2720, 3007, 3052
BseMI GCAATG 5 cut(s) 104, 186, 613, 1727, 2580
BseRI GAGGAG 1 cut(s) 3074
BseSI GKGCMC 1 cut(s) 3172
BseXI GCAGC 7 cut(s) 43, 85, 1235, 1970, 2597, 3023, 3227
BsgI GTGCAG 4 cut(s) 50, 282, 1977, 3234
Bsh1285I CGRYCG 1 cut(s) 1742
BshFI GGCC 3 cut(s) 612, 1309, 3000
BshNI GGYRCC 1 cut(s) 1882
BsiEI CGRYCG 1 cut(s) 1742
BsiHKAI GWGCWC 4 cut(s) 395, 1217, 2532, 3172
BsiHKCI CYCGRG 1 cut(s) 776
BsiSI CCGG 3 cut(s) 77, 2233, 2956
BslFI GGGAC 4 cut(s) 249, 936, 1578, 2158
BslI CCNNNNNNNGG 7 cut(s) 83, 900, 946, 1879, 2720, 3007, 3052
BsmAI GTCTC 7 cut(s) 234, 398, 569, 695, 884, 1424, 1922
BsmBI CGTCTC 1 cut(s) 234
BsmFI GGGAC 4 cut(s) 249, 936, 1578, 2158
BsmI GAATGC 2 cut(s) 1095, 1961
BsnI GGCC 3 cut(s) 612, 1309, 3000
Bso31I GGTCTC 1 cut(s) 884
BsoBI CYCGRG 1 cut(s) 776
Bsp1286I GDGCHC 4 cut(s) 395, 1217, 2532, 3172
Bsp1407I TGTACA 2 cut(s) 1131, 2245
Bsp1720I GCTNAGC 1 cut(s) 110
Bsp19I CCATGG 3 cut(s) 742, 1873, 2976
BspACI CCGC 5 cut(s) 71, 379, 1111, 2360, 3082
BspANI GGCC 3 cut(s) 612, 1309, 3000
BspHI TCATGA 1 cut(s) 195
BspLI GGNNCC 7 cut(s) 254, 692, 951, 952, 1039, 1884, 2983
BspMI ACCTGC 1 cut(s) 3200
BspPI GGATC 9 cut(s) 51, 1506, 1568, 1760, 1775, 2147, 2411, 2830, 3037
BspT107I GGYRCC 1 cut(s) 1882
BspTI CTTAAG 1 cut(s) 59
BspTNI GGTCTC 1 cut(s) 884
BsrDI GCAATG 5 cut(s) 104, 186, 613, 1727, 2580
BsrGI TGTACA 2 cut(s) 1131, 2245
BssECI CCNNGG 6 cut(s) 694, 742, 1027, 1873, 2450, 2976
BssNAI GTATAC 1 cut(s) 1466
BssNI GRCGYC 1 cut(s) 123
BssSI CACGAG 1 cut(s) 1932
BssT1I CCWWGG 6 cut(s) 694, 742, 1027, 1873, 2450, 2976
Bst1107I GTATAC 1 cut(s) 1466
Bst2BI CACGAG 1 cut(s) 1932
Bst2UI CCWGG 5 cut(s) 426, 735, 1620, 2241, 3255
Bst6I CTCTTC 2 cut(s) 342, 1920
BstACI GRCGYC 1 cut(s) 123
BstAFI CTTAAG 1 cut(s) 59
BstAUI TGTACA 2 cut(s) 1131, 2245
BstBAI YACGTR 3 cut(s) 133, 1297, 2280
BstC8I GCNNGC 5 cut(s) 102, 116, 906, 1258, 3227
BstDSI CCRYGG 3 cut(s) 742, 1873, 2976
BstEII GGTNACC 1 cut(s) 737
BstENI CCTNNNNNAGG 1 cut(s) 3050
BstF5I GGATG 6 cut(s) 587, 730, 2368, 2604, 2613, 2848
BstH2I RGCGCY 2 cut(s) 862, 1626
BstHHI GCGC 3 cut(s) 861, 1625, 3110
BstMAI GTCTC 7 cut(s) 234, 398, 569, 695, 884, 1424, 1922
BstMCI CGRYCG 1 cut(s) 1742
BstMWI GCNNNNNNNGC 7 cut(s) 37, 73, 106, 115, 2708, 2981, 3215
BstNI CCWGG 5 cut(s) 426, 735, 1620, 2241, 3255
BstNSI RCATGY 1 cut(s) 908
BstPAI GACNNNNGTC 1 cut(s) 2209
BstPI GGTNACC 1 cut(s) 737
BstSCI CCNGG 5 cut(s) 424, 733, 1618, 2239, 3253
BstSFI CTRYAG 1 cut(s) 980
BstSLI GKGCMC 1 cut(s) 3172
BstV1I GCAGC 7 cut(s) 43, 85, 1235, 1970, 2597, 3023, 3227
BstV2I GAAGAC 4 cut(s) 793, 2555, 2865, 3243
BstX2I RGATCY 7 cut(s) 1280, 1498, 1765, 1780, 2403, 2864, 3042
BstYI RGATCY 7 cut(s) 1280, 1498, 1765, 1780, 2403, 2864, 3042
BstZ17I GTATAC 1 cut(s) 1466
Bsu36I CCTNAGG 2 cut(s) 567, 2913
BsuRI GGCC 3 cut(s) 612, 1309, 3000
BtgI CCRYGG 3 cut(s) 742, 1873, 2976
BtsCI GGATG 6 cut(s) 587, 730, 2368, 2604, 2613, 2848
BtsIMutI CAGTG 6 cut(s) 606, 1141, 1302, 1797, 1799, 2593
BveI ACCTGC 1 cut(s) 3200
Cac8I GCNNGC 5 cut(s) 102, 116, 906, 1258, 3227
CaiI CAGNNNCTG 1 cut(s) 1639
CciI TCATGA 1 cut(s) 195
CfoI GCGC 3 cut(s) 861, 1625, 3110
Cfr13I GGNCC 7 cut(s) 139, 252, 746, 950, 1307, 2345, 2998
CseI GACGC 3 cut(s) 131, 1300, 2717
CsiI ACCWGGT 1 cut(s) 3253
Csp6I GTAC 7 cut(s) 534, 1068, 1132, 1298, 2230, 2246, 2320
CspCI CAANNNNNGTGG 2 cut(s) 3169, 3204
CviQI GTAC 7 cut(s) 534, 1068, 1132, 1298, 2230, 2246, 2320
DraI TTTAAA 1 cut(s) 2437
DraIII CACNNNGTG 1 cut(s) 1129
DrdI GACNNNNNNGTC 1 cut(s) 948
DseDI GACNNNNNNGTC 1 cut(s) 948
EaeI YGGCCR 1 cut(s) 610
Eam1104I CTCTTC 2 cut(s) 342, 1920
EarI CTCTTC 2 cut(s) 342, 1920
Ecl136II GAGCTC 3 cut(s) 393, 1215, 2530
Eco130I CCWWGG 6 cut(s) 694, 742, 1027, 1873, 2450, 2976
Eco24I GRGCYC 3 cut(s) 395, 1217, 2532
Eco31I GGTCTC 1 cut(s) 884
Eco47I GGWCC 5 cut(s) 139, 252, 746, 950, 2345
Eco53kI GAGCTC 3 cut(s) 393, 1215, 2530
Eco57I CTGAAG 4 cut(s) 366, 456, 507, 2681
Eco81I CCTNAGG 2 cut(s) 567, 2913
Eco88I CYCGRG 1 cut(s) 776
Eco91I GGTNACC 1 cut(s) 737
EcoICRI GAGCTC 3 cut(s) 393, 1215, 2530
EcoNI CCTNNNNNAGG 1 cut(s) 3050
EcoO109I RGGNCCY 1 cut(s) 950
EcoO65I GGTNACC 1 cut(s) 737
EcoRI GAATTC 1 cut(s) 1795
EcoRII CCWGG 5 cut(s) 424, 733, 1618, 2239, 3253
EcoT14I CCWWGG 6 cut(s) 694, 742, 1027, 1873, 2450, 2976
EcoT22I ATGCAT 1 cut(s) 1272
EcoT38I GRGCYC 3 cut(s) 395, 1217, 2532
ErhI CCWWGG 6 cut(s) 694, 742, 1027, 1873, 2450, 2976
Esp3I CGTCTC 1 cut(s) 234
FalI AAGNNNNNCTT 6 cut(s) 1083, 1115, 1524, 1556, 2434, 2466
FaqI GGGAC 4 cut(s) 249, 936, 1578, 2158
FauNDI CATATG 1 cut(s) 2050
FbaI TGATCA 2 cut(s) 198, 319
FblI GTMKAC 2 cut(s) 336, 1465
FokI GGATG 6 cut(s) 574, 717, 2375, 2611, 2620, 2855
FriOI GRGCYC 3 cut(s) 395, 1217, 2532
GlaI GCGC 3 cut(s) 860, 1624, 3109
GsuI CTGGAG 3 cut(s) 834, 1823, 3262
HaeII RGCGCY 2 cut(s) 862, 1626
HaeIII GGCC 3 cut(s) 612, 1309, 3000
HapII CCGG 3 cut(s) 77, 2233, 2956
HgaI GACGC 3 cut(s) 131, 1300, 2717
HhaI GCGC 3 cut(s) 861, 1625, 3110
Hin1I GRCGYC 1 cut(s) 123
Hin6I GCGC 3 cut(s) 859, 1623, 3108
HinP1I GCGC 3 cut(s) 859, 1623, 3108
HincII GTYRAC 1 cut(s) 2905
HindII GTYRAC 1 cut(s) 2905
HindIII AAGCTT 3 cut(s) 489, 1917, 2566
HpaII CCGG 3 cut(s) 77, 2233, 2956
Hpy99I CGWCG 4 cut(s) 86, 945, 1997, 2711
HpyCH4IV ACGT 7 cut(s) 132, 1296, 1954, 2279, 2350, 2907, 3126
HpyF10VI GCNNNNNNNGC 7 cut(s) 37, 73, 106, 115, 2708, 2981, 3215
HpySE526I ACGT 7 cut(s) 132, 1296, 1954, 2279, 2350, 2907, 3126
Hsp92I GRCGYC 1 cut(s) 123
HspAI GCGC 3 cut(s) 859, 1623, 3108
KflI GGGWCCC 1 cut(s) 950
Ksp22I TGATCA 2 cut(s) 198, 319
LmnI GCTCC 2 cut(s) 661, 2981
Lsp1109I GCAGC 7 cut(s) 43, 85, 1235, 1970, 2597, 3023, 3227
LweI GCATC 4 cut(s) 96, 529, 1210, 1257
MabI ACCWGGT 1 cut(s) 3253
MaeII ACGT 7 cut(s) 132, 1296, 1954, 2279, 2350, 2907, 3126
MaeIII GTNAC 9 cut(s) 286, 706, 737, 1121, 1145, 1196, 1360, 1380, 1905
MfeI CAATTG 1 cut(s) 2678
MflI RGATCY 7 cut(s) 1280, 1498, 1765, 1780, 2403, 2864, 3042
MhlI GDGCHC 4 cut(s) 395, 1217, 2532, 3172
MlsI TGGCCA 1 cut(s) 612
MluNI TGGCCA 1 cut(s) 612
MlyI GAGTC 8 cut(s) 17, 271, 351, 571, 1436, 1924, 2306, 3282
MmeI TCCRAC 4 cut(s) 1684, 2180, 2287, 2406
Mox20I TGGCCA 1 cut(s) 612
Mph1103I ATGCAT 1 cut(s) 1272
MroXI GAANNNNTTC 2 cut(s) 1095, 2762
MscI TGGCCA 1 cut(s) 612
MseI TTAA 7 cut(s) 60, 803, 827, 1082, 2100, 2436, 2727
MslI CAYNNNNRTG 3 cut(s) 735, 1473, 2253
Msp20I TGGCCA 1 cut(s) 612
MspA1I CMGCKG 2 cut(s) 379, 2422
MspCI CTTAAG 1 cut(s) 59
MspI CCGG 3 cut(s) 77, 2233, 2956
MspR9I CCNGG 5 cut(s) 426, 735, 1620, 2241, 3255
MssI GTTTAAAC 1 cut(s) 2437
MunI CAATTG 1 cut(s) 2678
Mva1269I GAATGC 2 cut(s) 1095, 1961
MvaI CCWGG 5 cut(s) 426, 735, 1620, 2241, 3255
MwoI GCNNNNNNNGC 7 cut(s) 37, 73, 106, 115, 2708, 2981, 3215
NcoI CCATGG 3 cut(s) 742, 1873, 2976
NdeI CATATG 1 cut(s) 2050
NlaIV GGNNCC 7 cut(s) 254, 692, 951, 952, 1039, 1884, 2983
NmuCI GTSAC 4 cut(s) 706, 737, 1121, 1380
NsiI ATGCAT 1 cut(s) 1272
NspI RCATGY 1 cut(s) 908
OliI CACNNNNGTG 1 cut(s) 2253
PaeI GCATGC 1 cut(s) 908
PaeR7I CTCGAG 1 cut(s) 776
PagI TCATGA 1 cut(s) 195
PaqCI CACCTGC 1 cut(s) 3200
PcsI WCGNNNNNNNCGW 1 cut(s) 129
PctI GAATGC 2 cut(s) 1095, 1961
PdmI GAANNNNTTC 2 cut(s) 1095, 2762
PfeI GAWTC 7 cut(s) 183, 218, 1735, 2126, 2236, 2795, 3004
PflMI CCANNNNNTGG 1 cut(s) 900
PleI GAGTC 8 cut(s) 17, 270, 351, 571, 1435, 1924, 2305, 3281
PmeI GTTTAAAC 1 cut(s) 2437
PpsI GAGTC 8 cut(s) 17, 270, 351, 571, 1435, 1924, 2305, 3281
Ppu21I YACGTR 3 cut(s) 133, 1297, 2280
PpuMI RGGWCCY 1 cut(s) 950
PshAI GACNNNNGTC 1 cut(s) 2209
Psp124BI GAGCTC 3 cut(s) 395, 1217, 2532
Psp5II RGGWCCY 1 cut(s) 950
Psp6I CCWGG 5 cut(s) 424, 733, 1618, 2239, 3253
PspEI GGTNACC 1 cut(s) 737
PspGI CCWGG 5 cut(s) 424, 733, 1618, 2239, 3253
PspN4I GGNNCC 7 cut(s) 254, 692, 951, 952, 1039, 1884, 2983
PspPI GGNCC 7 cut(s) 139, 252, 746, 950, 1307, 2345, 2998
PspPPI RGGWCCY 1 cut(s) 950
PspXI VCTCGAGB 1 cut(s) 776
PstNI CAGNNNCTG 1 cut(s) 1639
PsuI RGATCY 7 cut(s) 1280, 1498, 1765, 1780, 2403, 2864, 3042
PvuII CAGCTG 1 cut(s) 2422
RsaI GTAC 7 cut(s) 535, 1069, 1133, 1299, 2231, 2247, 2321
RsaNI GTAC 7 cut(s) 534, 1068, 1132, 1298, 2230, 2246, 2320
RseI CAYNNNNRTG 3 cut(s) 735, 1473, 2253
SacI GAGCTC 3 cut(s) 395, 1217, 2532
SaqAI TTAA 7 cut(s) 60, 803, 827, 1082, 2100, 2436, 2727
Sau96I GGNCC 7 cut(s) 139, 252, 746, 950, 1307, 2345, 2998
ScaI AGTACT 1 cut(s) 535
SchI GAGTC 8 cut(s) 17, 271, 351, 571, 1436, 1924, 2306, 3282
ScrFI CCNGG 5 cut(s) 426, 735, 1620, 2241, 3255
SduI GDGCHC 4 cut(s) 395, 1217, 2532, 3172
SexAI ACCWGGT 1 cut(s) 3253
SfaNI GCATC 4 cut(s) 96, 529, 1210, 1257
SfcI CTRYAG 1 cut(s) 980
Sfr274I CTCGAG 1 cut(s) 776
SinI GGWCC 5 cut(s) 139, 252, 746, 950, 2345
SlaI CTCGAG 1 cut(s) 776
SmiMI CAYNNNNRTG 3 cut(s) 735, 1473, 2253
SmlI CTYRAG 6 cut(s) 59, 327, 396, 776, 1589, 2407
SmoI CTYRAG 6 cut(s) 59, 327, 396, 776, 1589, 2407
SpeI ACTAGT 2 cut(s) 290, 530
SphI GCATGC 1 cut(s) 908
SsiI CCGC 5 cut(s) 71, 379, 1111, 2360, 3082
SspI AATATT 2 cut(s) 801, 3019
SstI GAGCTC 3 cut(s) 395, 1217, 2532
StyD4I CCNGG 5 cut(s) 424, 733, 1618, 2239, 3253
StyI CCWWGG 6 cut(s) 694, 742, 1027, 1873, 2450, 2976
TaiI ACGT 7 cut(s) 135, 1299, 1957, 2282, 2353, 2910, 3129
TaqII GACCGA 1 cut(s) 954
TatI WGTACW 3 cut(s) 533, 1131, 2245
TauI GCSGC 3 cut(s) 382, 1114, 3085
TfiI GAWTC 7 cut(s) 183, 218, 1735, 2126, 2236, 2795, 3004
Tru1I TTAA 7 cut(s) 60, 803, 827, 1082, 2100, 2436, 2727
Tru9I TTAA 7 cut(s) 60, 803, 827, 1082, 2100, 2436, 2727
TscAI CASTG 6 cut(s) 606, 1141, 1309, 1797, 1806, 2600
TseFI GTSAC 4 cut(s) 706, 737, 1121, 1380
TseI GCWGC 7 cut(s) 31, 73, 1223, 1958, 2585, 3011, 3215
Tsp45I GTSAC 4 cut(s) 706, 737, 1121, 1380
TspGWI ACGGA 1 cut(s) 16
TspRI CASTG 6 cut(s) 606, 1141, 1309, 1797, 1806, 2600
Van91I CCANNNNNTGG 1 cut(s) 900
Vha464I CTTAAG 1 cut(s) 59
VneI GTGCAC 1 cut(s) 3168
VpaK11BI GGWCC 5 cut(s) 139, 252, 746, 950, 2345
XagI CCTNNNNNAGG 1 cut(s) 3050
XapI RAATTY 8 cut(s) 144, 868, 1019, 1241, 1795, 2112, 2615, 2877
XbaI TCTAGA 2 cut(s) 239, 2518
XceI RCATGY 1 cut(s) 908
XcmI CCANNNNNNNNNTGG 2 cut(s) 2448, 2480
XhoI CTCGAG 1 cut(s) 776
XmiI GTMKAC 2 cut(s) 336, 1465
XmnI GAANNNNTTC 2 cut(s) 1095, 2762
ZrmI AGTACT 1 cut(s) 535
Zsp2I ATGCAT 1 cut(s) 1272
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.