Rroxscaffold_2G00149810
ERF Family

Probably involved in the RNA silencing pathway and required for the generation of small interfering RNAs (siRNAs)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000505
Physical Location & Seq
Reverse (-)
87332407 .. 87342272
9866 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00149810.1

Sequence Viewer

Length: 948 bp
ATGCCAGATATGCTATATTCTTCTCTTGCAAGGGACCACGTAATGTTGCTGATGAAATTGGTGGTGGAGATTTTGATGGTGATCTGTATTGGGTCTCAAGAAACCCTCAGCTATTGGAATGGTACAAACCAAGTGAACCTTGGAACGAAGGATCAAGATCAACTCAAAAGGTTGCCAGTAATAACTGAGCTTTCCACTGAAGATCTTGACGCTGATATGTTTAGATTGTTCTTGAAAAATAGGTTTCAACCAAGTTTTGTTATGAGTGAGGCAGCTAATAACTGGCTTGCTTGGATGGACTGGTTCTTGACTTTGGGTGATAGTAATGAGAAGAAGGACGTAATGGACAAGATACTGAGGTTGGTTGATATATACTACATCGCTCTAGATGCAGGGAAGAAAGGTGTAAAGGTTGAAGTTCCAAAAGATTTGAAGGTGTCACTTTTCCCTCATTACATGGACAAGAAAAAAGAAGAAGAAACTTACATTTCATCTTCGATTTTGGGAGAAATTTATGATGAAGTTGATAAATGTCAAGCAAAAATAATTTTTCTCAAAGAAATCAAGAAACTTCCTATTTTTGATATTAATCAAGTCCCCGAACTCTGCTTGGCCAAATGGAAGAAACGTTATGGACGGTACAGGACTGAAATGAGCTCAGCCATGGATGAGGATGGAGATCAGGCTGCCATTGTCATAAGAAAGTATAAAAAGAAGCTGTATAAGGCTGATGACTTTGAGCACAGTTCAAGGCCGATGGATGAAATACTGGACGAGGCACAAGCTATATATCATGTATGTTATGACCATGCTATGGATAAAAGAGATGTGAAAAAATGTTCCTTTGCATGGAGAGTTGCAGGTAAAGCTCTGTGCGAGCTTCACGCTAGGAAAAAGGATGAGAAGTGGTTAAACACTGCCCCTTCTGTTTTGAAAGATCTTTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RdRP

Protein Analysis

315

Amino Acids

36.69

Weight (kDa)

6.35

Isoelectric Point (pI)

36.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RdRP PF05183 80 - 175 3.3e-12 RNA dependent RNA polymerase
RdRP_head PF26253 227 - 302 1.3e-12 RdRP, head domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000505)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G19910 AT2G19910 AT2G19920 AT2G19920 AT2G19920 AT2G19920 AT2G19930 AT2G19930 AT2G19930
fragaria_vesca FvH4_1g05980 FvH4_1g05990 FvH4_1g05990 FvH4_1g09718
malus_domestica MD02G1064400.v1.1 MD15G1195300.v1.1 MD15G1195400.v1.1
prunus_persica Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1
pyrus_communis pycom02g05070 pycom15g17460
rosa_chinensis RchiOBHm_Chr2g0091671 RchiOBHm_Chr2g0091681 RchiOBHm_Chr2g0091691 RchiOBHm_Chr2g0091701 RchiOBHm_Chr2g0091711 RchiOBHm_Chr2g0091721 RchiOBHm_Chr2g0091731 RchiOBHm_Chr5g0014401
rosa_laevigata RLG00000013772 RLG00000016242 RLG00000016244 RLG00000016245 RLG00000016246 RLG00000032105
rosa_multiflora Rmu_co8374041.1_g000001 Rmu_co8478121.1_g000001 Rmu_sc0000897.1_g000005 Rmu_sc0000897.1_g000009
rosa_roxburghii Rroxscaffold_1G00062180 Rroxscaffold_1G00062190 Rroxscaffold_1G00068800 Rroxscaffold_2G00149790 Rroxscaffold_2G00149800 Rroxscaffold_2G00149810
rosa_rugosa Rorug02G0019600 Rorug02G0019700 Rorug02G0019800 Rorug02G0019900 Rorug02G0019900 Rorug02G0020000 Rorug02G0020000 Rorug02G0020100 Rorug02G0020100 Rorug02G0020200 Rorug05G0017800
rosa_samantha Rh2AG064700 Rh2AG064800 Rh2AG064900 Rh2BG063800 Rh2BG063900 Rh2BG064000 Rh2BG064100 Rh2CG065500 Rh2CG065600 Rh2CG065700 Rh2DG063900 Rh2DG064000 Rh2DG064100 Rh2DG064200 Rh2DG381700 Rh5AG056700 Rh5AG056800 Rh5AG056900 Rh5AG112600 Rh5BG109300 Rh5CG063800 Rh5CG121100 Rh5DG053100 Rh5DG108300 Rh6CG100100
rosa_wichuraiana Rw2G005560 Rw2G005580 Rw2G005590 Rw5G005370 Rw5G009770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 851
AccB7I CCANNNNNTGG 1 cut(s) 814
AclI AACGTT 1 cut(s) 628
AclWI GGATC 1 cut(s) 159
AcoI YGGCCR 1 cut(s) 612
AcsI RAATTY 1 cut(s) 510
AcuI CTGAAG 1 cut(s) 219
AfaI GTAC 2 cut(s) 124, 641
AfiI CCNNNNNNNGG 2 cut(s) 814, 849
AgsI TTSAA 6 cut(s) 235, 248, 416, 433, 750, 934
AluBI AGCT 8 cut(s) 111, 190, 275, 657, 718, 785, 869, 880
AluI AGCT 8 cut(s) 111, 190, 275, 657, 718, 785, 869, 880
Alw21I GWGCWC 2 cut(s) 659, 744
Alw26I GTCTC 1 cut(s) 99
AlwI GGATC 1 cut(s) 159
AoxI GGCC 2 cut(s) 612, 752
ApeKI GCWGC 2 cut(s) 272, 686
ApoI RAATTY 1 cut(s) 510
AseI ATTAAT 1 cut(s) 588
AspS9I GGNCC 1 cut(s) 34
AsuHPI GGTGA 2 cut(s) 91, 329
AvaII GGWCC 1 cut(s) 34
BaeI ACNNNNGTAYC 2 cut(s) 344, 377
BalI TGGCCA 1 cut(s) 614
BanII GRGCYC 1 cut(s) 659
Bbv12I GWGCWC 2 cut(s) 659, 744
BbvCI CCTCAGC 1 cut(s) 107
BbvI GCAGC 2 cut(s) 284, 673
BccI CCATC 4 cut(s) 70, 289, 668, 751
BcoDI GTCTC 1 cut(s) 99
BfaI CTAG 2 cut(s) 386, 888
BfuAI ACCTGC 1 cut(s) 851
BglII AGATCT 2 cut(s) 202, 937
BisI GCNGC 2 cut(s) 273, 687
BlpI GCTNAGC 1 cut(s) 658
BlsI GCNGC 2 cut(s) 274, 688
Bme18I GGWCC 1 cut(s) 34
BmgT120I GGNCC 1 cut(s) 34
BmiI GGNNCC 1 cut(s) 35
BmsI GCATC 1 cut(s) 379
Bpu10I CCTNAGC 1 cut(s) 107
Bpu1102I GCTNAGC 1 cut(s) 658
BpuEI CTTGAG 1 cut(s) 81
BsaAI YACGTR 1 cut(s) 40
BsaBI GATNNNNATC 4 cut(s) 80, 156, 588, 678
BsaI GGTCTC 1 cut(s) 99
BsaJI CCNNGG 2 cut(s) 139, 663
Bsc4I CCNNNNNNNGG 2 cut(s) 814, 849
Bse1I ACTGG 4 cut(s) 176, 287, 305, 774
Bse8I GATNNNNATC 4 cut(s) 80, 156, 588, 678
BseDI CCNNGG 2 cut(s) 139, 663
BseGI GGATG 5 cut(s) 300, 673, 679, 766, 904
BseJI GATNNNNATC 4 cut(s) 80, 156, 588, 678
BseLI CCNNNNNNNGG 2 cut(s) 814, 849
BseMII CTCAG 4 cut(s) 121, 177, 347, 672
BseNI ACTGG 4 cut(s) 176, 287, 305, 774
BseXI GCAGC 2 cut(s) 284, 673
BshFI GGCC 2 cut(s) 614, 754
BsiHKAI GWGCWC 2 cut(s) 659, 744
BslFI GGGAC 2 cut(s) 47, 581
BslI CCNNNNNNNGG 2 cut(s) 814, 849
BsmAI GTCTC 1 cut(s) 99
BsmFI GGGAC 2 cut(s) 47, 581
BsnI GGCC 2 cut(s) 614, 754
Bso31I GGTCTC 1 cut(s) 99
Bsp1286I GDGCHC 2 cut(s) 659, 744
Bsp143I GATC 6 cut(s) 81, 151, 157, 202, 679, 937
Bsp1720I GCTNAGC 1 cut(s) 658
Bsp19I CCATGG 1 cut(s) 663
BspANI GGCC 2 cut(s) 614, 754
BspCNI CTCAG 4 cut(s) 120, 178, 348, 671
BspLI GGNNCC 1 cut(s) 35
BspMI ACCTGC 1 cut(s) 851
BspPI GGATC 1 cut(s) 159
BspTNI GGTCTC 1 cut(s) 99
BsrI ACTGG 4 cut(s) 176, 287, 305, 774
BssECI CCNNGG 2 cut(s) 139, 663
BssMI GATC 6 cut(s) 81, 151, 157, 202, 679, 937
BssT1I CCWWGG 2 cut(s) 139, 663
Bst4CI ACNGT 2 cut(s) 639, 746
BstBAI YACGTR 1 cut(s) 40
BstC8I GCNNGC 2 cut(s) 288, 878
BstDEI CTNAG 4 cut(s) 107, 186, 356, 658
BstDSI CCRYGG 1 cut(s) 663
BstF5I GGATG 5 cut(s) 300, 673, 679, 766, 904
BstKTI GATC 6 cut(s) 84, 154, 160, 205, 682, 940
BstMAI GTCTC 1 cut(s) 99
BstMBI GATC 6 cut(s) 81, 151, 157, 202, 679, 937
BstMWI GCNNNNNNNGC 3 cut(s) 10, 389, 866
BstV1I GCAGC 2 cut(s) 284, 673
BstX2I RGATCY 2 cut(s) 202, 937
BstYI RGATCY 2 cut(s) 202, 937
BsuRI GGCC 2 cut(s) 614, 754
BtgI CCRYGG 1 cut(s) 663
BtgZI GCGATG 1 cut(s) 364
BtsCI GGATG 5 cut(s) 300, 673, 679, 766, 904
BtsI GCAGTG 1 cut(s) 915
BtsIMutI CAGTG 2 cut(s) 195, 915
BveI ACCTGC 1 cut(s) 851
Cac8I GCNNGC 2 cut(s) 288, 878
Cfr13I GGNCC 1 cut(s) 34
CseI GACGC 1 cut(s) 218
Csp6I GTAC 2 cut(s) 123, 640
CviAII CATG 5 cut(s) 457, 664, 794, 809, 849
CviQI GTAC 2 cut(s) 123, 640
DdeI CTNAG 4 cut(s) 107, 186, 356, 658
DpnI GATC 6 cut(s) 83, 153, 159, 204, 681, 939
DpnII GATC 6 cut(s) 81, 151, 157, 202, 679, 937
EaeI YGGCCR 1 cut(s) 612
Ecl136II GAGCTC 1 cut(s) 657
Eco130I CCWWGG 2 cut(s) 139, 663
Eco24I GRGCYC 1 cut(s) 659
Eco31I GGTCTC 1 cut(s) 99
Eco47I GGWCC 1 cut(s) 34
Eco53kI GAGCTC 1 cut(s) 657
Eco57I CTGAAG 1 cut(s) 219
EcoICRI GAGCTC 1 cut(s) 657
EcoT14I CCWWGG 2 cut(s) 139, 663
EcoT38I GRGCYC 1 cut(s) 659
ErhI CCWWGG 2 cut(s) 139, 663
FaeI CATG 5 cut(s) 460, 667, 797, 812, 852
FalI AAGNNNNNCTT 2 cut(s) 123, 155
FaqI GGGAC 2 cut(s) 47, 581
FatI CATG 5 cut(s) 456, 663, 793, 808, 848
Fnu4HI GCNGC 2 cut(s) 273, 687
FokI GGATG 5 cut(s) 307, 680, 686, 773, 911
FriOI GRGCYC 1 cut(s) 659
Fsp4HI GCNGC 2 cut(s) 273, 687
FspBI CTAG 2 cut(s) 386, 888
GluI GCNGC 2 cut(s) 273, 687
HaeIII GGCC 2 cut(s) 614, 754
HgaI GACGC 1 cut(s) 218
Hin1II CATG 5 cut(s) 460, 667, 797, 812, 852
HphI GGTGA 2 cut(s) 91, 329
Hpy166II GTNNAC 1 cut(s) 136
Hpy188III TCNNGA 7 cut(s) 98, 155, 206, 232, 307, 386, 565
Hpy8I GTNNAC 1 cut(s) 136
HpyAV CCTTC 4 cut(s) 142, 328, 427, 933
HpyCH4III ACNGT 2 cut(s) 639, 746
HpyCH4IV ACGT 3 cut(s) 39, 339, 628
HpyCH4V TGCA 4 cut(s) 29, 392, 848, 860
HpyF10VI GCNNNNNNNGC 3 cut(s) 10, 389, 866
HpyF3I CTNAG 4 cut(s) 107, 186, 356, 658
HpySE526I ACGT 3 cut(s) 39, 339, 628
Hsp92II CATG 5 cut(s) 460, 667, 797, 812, 852
Kzo9I GATC 6 cut(s) 81, 151, 157, 202, 679, 937
LpnPI CCDG 9 cut(s) 18, 189, 268, 286, 378, 628, 668, 755, 846
Lsp1109I GCAGC 2 cut(s) 284, 673
LweI GCATC 1 cut(s) 379
MaeI CTAG 2 cut(s) 386, 888
MaeII ACGT 3 cut(s) 39, 339, 628
MaeIII GTNAC 1 cut(s) 438
MalI GATC 6 cut(s) 83, 153, 159, 204, 681, 939
MboI GATC 6 cut(s) 81, 151, 157, 202, 679, 937
MboII GAAGA 8 cut(s) 12, 212, 343, 409, 485, 486, 488, 634
MflI RGATCY 2 cut(s) 202, 937
MhlI GDGCHC 2 cut(s) 659, 744
MlsI TGGCCA 1 cut(s) 614
MluCI AATT 3 cut(s) 56, 510, 546
MluNI TGGCCA 1 cut(s) 614
MnlI CCTC 6 cut(s) 116, 262, 351, 459, 664, 769
Mox20I TGGCCA 1 cut(s) 614
MscI TGGCCA 1 cut(s) 614
MseI TTAA 2 cut(s) 588, 911
Msp20I TGGCCA 1 cut(s) 614
MwoI GCNNNNNNNGC 3 cut(s) 10, 389, 866
NcoI CCATGG 1 cut(s) 663
NdeII GATC 6 cut(s) 81, 151, 157, 202, 679, 937
NlaIII CATG 5 cut(s) 460, 667, 797, 812, 852
NlaIV GGNNCC 1 cut(s) 35
NmuCI GTSAC 1 cut(s) 438
PflMI CCANNNNNTGG 1 cut(s) 814
PkrI GCNGC 2 cut(s) 274, 688
Ppu21I YACGTR 1 cut(s) 40
PshBI ATTAAT 1 cut(s) 588
Psp124BI GAGCTC 1 cut(s) 659
Psp1406I AACGTT 1 cut(s) 628
PspN4I GGNNCC 1 cut(s) 35
PspPI GGNCC 1 cut(s) 34
PsuI RGATCY 2 cut(s) 202, 937
RsaI GTAC 2 cut(s) 124, 641
RsaNI GTAC 2 cut(s) 123, 640
SacI GAGCTC 1 cut(s) 659
SaqAI TTAA 2 cut(s) 588, 911
SatI GCNGC 2 cut(s) 273, 687
Sau3AI GATC 6 cut(s) 81, 151, 157, 202, 679, 937
Sau96I GGNCC 1 cut(s) 34
SduI GDGCHC 2 cut(s) 659, 744
SfaNI GCATC 1 cut(s) 379
SinI GGWCC 1 cut(s) 34
SmlI CTYRAG 1 cut(s) 96
SmoI CTYRAG 1 cut(s) 96
Sse9I AATT 3 cut(s) 56, 510, 546
SspMI CTAG 2 cut(s) 386, 888
SstI GAGCTC 1 cut(s) 659
StyI CCWWGG 2 cut(s) 139, 663
TaaI ACNGT 2 cut(s) 639, 746
TaiI ACGT 3 cut(s) 42, 342, 631
TaqI TCGA 1 cut(s) 497
TasI AATT 3 cut(s) 56, 510, 546
Tru1I TTAA 2 cut(s) 588, 911
Tru9I TTAA 2 cut(s) 588, 911
TscAI CASTG 2 cut(s) 202, 922
TseFI GTSAC 1 cut(s) 438
TseI GCWGC 2 cut(s) 272, 686
Tsp45I GTSAC 1 cut(s) 438
TspDTI ATGAA 4 cut(s) 68, 480, 534, 777
TspRI CASTG 2 cut(s) 202, 922
Van91I CCANNNNNTGG 1 cut(s) 814
VpaK11BI GGWCC 1 cut(s) 34
VspI ATTAAT 1 cut(s) 588
XapI RAATTY 1 cut(s) 510
XbaI TCTAGA 1 cut(s) 385
XcmI CCANNNNNNNNNTGG 1 cut(s) 137
XspI CTAG 2 cut(s) 386, 888
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.