MD15G1195400.v1.1
ERF Family

Probably involved in the RNA silencing pathway and required for the generation of small interfering RNAs (siRNAs)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0000505
Physical Location & Seq
Forward (+)
15466809 .. 15475523
8715 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1195400.v1.1.491

Sequence Viewer

Length: 3285 bp
ATGGATTCTGCTTCTGTGAAAACGTTCCTGCTCGATGTTAGAGCTTCTGCGAATTCGTTGTTGTCGCAGTACGAGCCTCTGGTTCTGGTTTTTGCTGCTCTCTTTGCTCTCCTTGTCTCTTCCGCGCTTCAATGGACTATCAATGGCCTGCAGGAGAAGGGACTCAAAGCCACACTCCTAGGGTTATTCATGAGCTCCATCAAGTTGGTTCCTGGCGTCAAGAGTTACATCGAAGCTGAGAAGCAAAAGATGGCCATGGCGGATTCCACCGTCTCAATAAAAAACCCATCTTTCTCTCCTCCTCCGTCATATATGGCCGCCGCCGGCGGTCTAAATTTGCAACCGCAACCATACATTTTCCTGCCGCAGTCGGTGGAGCAGCTGCTGCGTCGAATTTGCGAAGAAAAAAAGCGTCCCTGGCCAGAAGACGACGTTTTGCGGAGGCTGAGTTTTCTGACGGAGAAGCAGGCGCTCGAGCTCCTCCGTCAGATCGCCCGCGCCAAAGAAGTTAAGACTCTCAACGGCTACATCATCTGGCTCTGTCAGACCCAGTATTCCTCCTCCTCTCCTTCGCAATCTTCCCTTGCTTCTCAATTCCAAGGTGTGACTCTCAATCATCGCTGCTCCTCAGCTGTTGAGCAACCTGTGGCTATAGGCAATGCAGAAGCAGAGCTGGAAGCTTTGGGAGAACTTGAATTCAGAAAACAGTTTCTGATTCTAAATTACTCTGGAGGGTATGGTTTATCTCTCTGTTTGTTTACCGGAATGCAGTTGGGAAGTGTTATACCGGCGGAGACTATTAGGAGTTGGAAAGATCTGCCAATGCATATATTTGAGACCAGAGTTTGGGAAGCTTTGGGTCGCAATTATATTGGCAAGGAAGATCGGCGTTTGACTTTTGAATGGGATTCTGGAAAAACACACCTCTATCATTGTTATGTTTCTTTGGATGGGAGTTGCAAATTCAAGGGTCCATTTCTAAACAAAGAAAGAACTCTCCTACAGAAAGTTTTAGGAGACGACAATGTTTTAATGATGAAATTTGCTGATGAGGGGAATGGGACAGGTCCCTCAAATTCTTACAATAATAAGTATGTTACATATCGCAAGGTTGCAAGAGAAGGGATTCTTGTAGGGTTGCGACGATTTTGTTTCTTTGTGTTCAAAGATGGTGGTAAAGAGGAAAAGAAAAAAAACCCAACTTCATCACCCGTGAAGTGTTATTTTGTCCGTATGGACTCTGATGCTTCAATTGACAGGGATGTATGGTATATGTTAACTAACAAAACAATTTATGAAGCAAGATCTCTTTTCATGCATGCGCATACTGTGTCTAGTGTGCCCACCTACATGGCTAGGTTTTCGCTCATTCTGTCAAAGACAATCAGATTTCCAGTAGATCTGTCAGCTGTGAATGTTGAGTTTGTTGATGAGGAATATTGTAGGGATGAATCTGGTAACCTTATTTACAGAGATGACAAACCACTTATACATACAGATGGCACTGGATTTATATCTGAGGATTTGGCTTTACTTTGTCCGAAGAACATATCCAATGGAAAACGGATCAGTAATCAAAAAATCGAGAGACTTCCGAATCATAATGAACTTGATGGCAACGGTCTGGAAAAGAAACAGCCAGGTTCTAGAATCGGGGAACCGCCTTTGCTCATACAGTTCCGACTGTTTTACAAAGGCTGTGCTGTCAAGGGAACATTTCTTGTCAACAAAATGCTGCCACCGAAAACAGTCCAGATTCGACCTCAAATGGTTAAAGTTCAGATAGATCCAGAACTTTCAGATAATGAAACAGTGAATGCATTAGAGATAGTTGGAACAAGTATTCCACCTGGGAAGTCATATTTATCGAGGAATTTAATTGCGCTTCTATTCCATGGAGGGGTGCCAAAAGAATATTTCTTGGAAATACTTGAGAGAGCTTTGGAAGATGCTCATAGTGTTTTCTGCAATGCGCGTACCGCACTCAAAGTTGCCGTTAACAATGGTGAGATTGATGACAATTCCAATACGGCAAGAATGATCCTATGCGGTATTCCCTTAGACGAATCATATTTGCAATATCGTCTATCGATATTAATAAAAGAGGAAATGAAAGGTCTTAGAAGAGGGAAACTGTATGTTCCCGATTGCTATTACTTGATGGGGACAGCTGATCCAACCGGGATTCTAGAGAAAGACGAAGTTTGCATTATCCTTGACAGCGGACAAATTTCGGGCAAGGTATTAGTGTATCGGAATCCAGGTTTGCACTTCGGGGATATTCATGTTTTGAAGGCCACTTATGTGAAGGAGTTAGAAGATGTTGTTGGAAATGCCAAGTATGCTATATTTTTCTCTCGCAAAGGTCCGCGTTCTGTAGCTGATGAAATAAGTGGTGGAGATTTTGATGGTGATCTCTACTGGGTTTCAAGAAACCCTCAGCTACTGGAGTATTTCAAACCCAGTGAACCTTGGGTTCCGGCTTCTTCAGCTCCTAAAGCTGCCAGTGTCAAACCAGCTAAGCTTTCGAAAGAAGACCTAGAAGAGCAGCTCATAAATCTGTTTTTGACAACTAGATTTAAACCAAGTTATGCAATGAGTGAGGCAGCTGATAGCTGGCTGGCTATGATGGATCGCTACTTGACCCGAGGAGATAGTAGCATTGATGAGAACTTGGTGAAGAACATACTGCGTTTGGTTGATATATACTATGATGCGCTAGATGCGCCAAAGAAGGGTGGAAAGGTCGAAGTTCCCAAAGACTTGAAGGTGTCGAAGTTTCCTCATTACATGGAAAAGAAAAATTCATACCGTTCAACTTCGATTTTAGGATCGATTTATGACAGGGTGAACGAGTACCAACCAGACGACCTCTCAACCAAAGAAGTTAAGAAACTTCCCATTTTCGACATTGAAGTCCCTGAGGAGTGCTTGATCAAATGGAGGGAACAATACAAACAGTACAGGCGTGACATGACCTCGGCATTGCGAGATGATGATCGAGAGGGGAAGAATCAGGCTGCCGGGGAGGTCATAACAAAGTATAGAGAGATTCTGTACGGTGGTGATGATTTCGACAAGAGCACAAGGCCGATGGGTGAAATATTTGACGAGGCGATCGCGATATATAATATCTGTTACGACTATGCCAGGAGTGCAGGTGTAAGTAAATGCGTGTTTGCATGGAAAGTTGCAGGGTCAGCTCTCATCAAACTTCACTTGATCAAACAAGGTGATGCATGCCCTTTTCTGGTGTCACCCGCTGTCTTGAAGGATATTCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RdRP

Protein Analysis

1095

Amino Acids

123.31

Weight (kDa)

7.68

Isoelectric Point (pI)

45.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
4HB_RdRP3_N PF26249 120 - 183 1.7e-08 RdRP3/4/5, N-terminal four helical bundle domain
RdRP PF05183 321 - 950 8.2e-103 RNA dependent RNA polymerase
RdRP_head PF26253 999 - 1074 4.8e-09 RdRP, head domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000505)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G19910 AT2G19910 AT2G19920 AT2G19920 AT2G19920 AT2G19920 AT2G19930 AT2G19930 AT2G19930
fragaria_vesca FvH4_1g05980 FvH4_1g05990 FvH4_1g05990 FvH4_1g09718
malus_domestica MD02G1064400.v1.1 MD15G1195300.v1.1 MD15G1195400.v1.1
prunus_persica Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1
pyrus_communis pycom02g05070 pycom15g17460
rosa_chinensis RchiOBHm_Chr2g0091671 RchiOBHm_Chr2g0091681 RchiOBHm_Chr2g0091691 RchiOBHm_Chr2g0091701 RchiOBHm_Chr2g0091711 RchiOBHm_Chr2g0091721 RchiOBHm_Chr2g0091731 RchiOBHm_Chr5g0014401
rosa_laevigata RLG00000013772 RLG00000016242 RLG00000016244 RLG00000016245 RLG00000016246 RLG00000032105
rosa_multiflora Rmu_co8374041.1_g000001 Rmu_co8478121.1_g000001 Rmu_sc0000897.1_g000005 Rmu_sc0000897.1_g000009
rosa_roxburghii Rroxscaffold_1G00062180 Rroxscaffold_1G00062190 Rroxscaffold_1G00068800 Rroxscaffold_2G00149790 Rroxscaffold_2G00149800 Rroxscaffold_2G00149810
rosa_rugosa Rorug02G0019600 Rorug02G0019700 Rorug02G0019800 Rorug02G0019900 Rorug02G0019900 Rorug02G0020000 Rorug02G0020000 Rorug02G0020100 Rorug02G0020100 Rorug02G0020200 Rorug05G0017800
rosa_samantha Rh2AG064700 Rh2AG064800 Rh2AG064900 Rh2BG063800 Rh2BG063900 Rh2BG064000 Rh2BG064100 Rh2CG065500 Rh2CG065600 Rh2CG065700 Rh2DG063900 Rh2DG064000 Rh2DG064100 Rh2DG064200 Rh2DG381700 Rh5AG056700 Rh5AG056800 Rh5AG056900 Rh5AG112600 Rh5BG109300 Rh5CG063800 Rh5CG121100 Rh5DG053100 Rh5DG108300 Rh6CG100100
rosa_wichuraiana Rw2G005560 Rw2G005580 Rw2G005590 Rw5G005370 Rw5G009770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 3149
AasI GACNNNNNNGTC 1 cut(s) 2915
Acc16I TGCGCA 1 cut(s) 1323
Acc36I ACCTGC 1 cut(s) 3149
AccB1I GGYRCC 1 cut(s) 1903
AccB7I CCANNNNNTGG 1 cut(s) 846
AccII CGCG 5 cut(s) 125, 498, 1975, 2371, 3122
AclI AACGTT 1 cut(s) 23
AclWI GGATC 6 cut(s) 1574, 1781, 2035, 2168, 2640, 2839
AcoI YGGCCR 3 cut(s) 252, 315, 419
AcuI CTGAAG 1 cut(s) 2472
AcyI GRCGYC 1 cut(s) 216
AfaI GTAC 5 cut(s) 71, 1978, 2858, 2963, 3059
AfiI CCNNNNNNNGG 4 cut(s) 846, 1900, 2735, 3250
AjnI CCWGG 6 cut(s) 211, 416, 1639, 1849, 2260, 3149
AjuI GAANNNNNNNTTGG 2 cut(s) 2310, 2342
AleI CACNNNNGTG 1 cut(s) 2303
AloI GAACNNNNNNTCC 2 cut(s) 1828, 1860
Alw21I GWGCWC 3 cut(s) 197, 480, 3086
Alw26I GTCTC 6 cut(s) 121, 277, 788, 830, 1011, 1582
AlwI GGATC 6 cut(s) 1574, 1781, 2035, 2168, 2640, 2839
AlwNI CAGNNNCTG 3 cut(s) 385, 712, 2446
Ama87I CYCGRG 2 cut(s) 473, 2646
AoxI GGCC 6 cut(s) 145, 252, 315, 419, 2295, 3089
AseI ATTAAT 1 cut(s) 2096
AsiSI GCGATCGC 1 cut(s) 3120
Asp700I GAANNNNTTC 2 cut(s) 23, 1125
AspA2I CCTAGG 1 cut(s) 178
AspLEI GCGC 8 cut(s) 127, 472, 500, 1324, 1885, 1975, 2719, 2728
AspS9I GGNCC 3 cut(s) 971, 1067, 2366
AsuC2I CCSGG 2 cut(s) 2182, 3025
AsuHPI GGTGA 9 cut(s) 1200, 2018, 2423, 2689, 2860, 3077, 3110, 3245, 3249
AsuII TTCGAA 1 cut(s) 2528
AvaI CYCGRG 2 cut(s) 473, 2646
AvaII GGWCC 3 cut(s) 971, 1067, 2366
AvrII CCTAGG 1 cut(s) 178
AxyI CCTNAGG 1 cut(s) 2922
BaeGI GKGCMC 1 cut(s) 1344
BalI TGGCCA 2 cut(s) 254, 421
BanI GGYRCC 1 cut(s) 1903
BanII GRGCYC 2 cut(s) 197, 480
BbsI GAAGAC 2 cut(s) 432, 2541
Bbv12I GWGCWC 3 cut(s) 197, 480, 3086
BbvCI CCTCAGC 2 cut(s) 628, 2439
BceAI ACGGC 3 cut(s) 538, 1979, 2046
BciT130I CCWGG 6 cut(s) 213, 418, 1641, 1851, 2262, 3151
BclI TGATCA 2 cut(s) 2934, 3222
BcnI CCSGG 2 cut(s) 2182, 3025
BcoDI GTCTC 6 cut(s) 121, 277, 788, 830, 1011, 1582
BfaI CTAG 8 cut(s) 179, 1335, 1356, 1647, 2189, 2540, 2574, 2720
BfmI CTRYAG 4 cut(s) 149, 651, 1001, 2376
BfoI RGCGCY 1 cut(s) 473
BfuAI ACCTGC 1 cut(s) 3149
BglII AGATCT 3 cut(s) 814, 1304, 1399
BlnI CCTAGG 1 cut(s) 178
BlpI GCTNAGC 1 cut(s) 2520
Bme1390I CCNGG 8 cut(s) 213, 418, 1641, 1851, 2182, 2262, 3025, 3151
Bme18I GGWCC 3 cut(s) 971, 1067, 2366
BmeT110I CYCGRG 2 cut(s) 473, 2646
BmgT120I GGNCC 3 cut(s) 971, 1067, 2366
BmiI GGNNCC 6 cut(s) 210, 972, 1069, 1659, 1905, 2478
BmrFI CCNGG 8 cut(s) 213, 418, 1641, 1851, 2182, 2262, 3025, 3151
BmrI ACTGGG 3 cut(s) 544, 2431, 2457
BmsI GCATC 5 cut(s) 1234, 1939, 2704, 2713, 3226
BmuI ACTGGG 3 cut(s) 544, 2431, 2457
BpiI GAAGAC 2 cut(s) 432, 2541
BpmI CTGGAG 2 cut(s) 750, 2468
Bpu10I CCTNAGC 2 cut(s) 628, 2439
Bpu1102I GCTNAGC 1 cut(s) 2520
Bpu14I TTCGAA 1 cut(s) 2528
BpuEI CTTGAG 1 cut(s) 1952
BpuMI CCSGG 2 cut(s) 2182, 3025
Bsa29I ATCGAT 2 cut(s) 2090, 2834
BsaBI GATNNNNATC 3 cut(s) 1513, 2412, 2997
BsaHI GRCGYC 1 cut(s) 216
BsaI GGTCTC 1 cut(s) 830
BsaWI WCCGGW 1 cut(s) 761
BsaXI ACNNNNNCTCC 4 cut(s) 433, 463, 3145, 3175
Bsc4I CCNNNNNNNGG 4 cut(s) 846, 1900, 2735, 3250
Bse118I RCCGGY 2 cut(s) 323, 787
Bse1I ACTGG 7 cut(s) 550, 1394, 1510, 2426, 2451, 2463, 2505
Bse21I CCTNAGG 1 cut(s) 2922
Bse3DI GCAATG 4 cut(s) 664, 1975, 2601, 2984
Bse8I GATNNNNATC 3 cut(s) 1513, 2412, 2997
BseBI CCWGG 6 cut(s) 213, 418, 1641, 1851, 2262, 3151
BseCI ATCGAT 2 cut(s) 2090, 2834
BseGI GGATG 3 cut(s) 955, 1267, 1453
BseJI GATNNNNATC 3 cut(s) 1513, 2412, 2997
BseLI CCNNNNNNNGG 4 cut(s) 846, 1900, 2735, 3250
BseMI GCAATG 4 cut(s) 664, 1975, 2601, 2984
BseMII CTCAG 6 cut(s) 228, 437, 642, 1509, 2453, 2913
BseNI ACTGG 7 cut(s) 550, 1394, 1510, 2426, 2451, 2463, 2505
BseRI GAGGAG 8 cut(s) 288, 291, 470, 550, 553, 616, 2664, 2939
BseSI GKGCMC 1 cut(s) 1344
BsgI GTGCAG 1 cut(s) 3177
Bsh1236I CGCG 5 cut(s) 125, 498, 1975, 2371, 3122
Bsh1285I CGRYCG 1 cut(s) 3120
BshFI GGCC 6 cut(s) 147, 254, 317, 421, 2297, 3091
BshNI GGYRCC 1 cut(s) 1903
BshVI ATCGAT 2 cut(s) 2090, 2834
BsiEI CGRYCG 1 cut(s) 3120
BsiHKAI GWGCWC 3 cut(s) 197, 480, 3086
BsiHKCI CYCGRG 2 cut(s) 473, 2646
BsiSI CCGG 6 cut(s) 324, 762, 788, 2181, 2480, 3024
BslFI GGGAC 6 cut(s) 174, 399, 1053, 1075, 2179, 2903
BslI CCNNNNNNNGG 4 cut(s) 846, 1900, 2735, 3250
BsmAI GTCTC 6 cut(s) 121, 277, 788, 830, 1011, 1582
BsmBI CGTCTC 2 cut(s) 277, 1011
BsmFI GGGAC 6 cut(s) 174, 399, 1053, 1075, 2179, 2903
BsmI GAATGC 2 cut(s) 771, 1822
BsnI GGCC 6 cut(s) 147, 254, 317, 421, 2297, 3091
Bso31I GGTCTC 1 cut(s) 830
BsoBI CYCGRG 2 cut(s) 473, 2646
Bsp119I TTCGAA 1 cut(s) 2528
Bsp1286I GDGCHC 4 cut(s) 197, 480, 1344, 3086
Bsp1720I GCTNAGC 1 cut(s) 2520
Bsp19I CCATGG 2 cut(s) 255, 1894
Bsp68I TCGCGA 1 cut(s) 3122
BspANI GGCC 6 cut(s) 147, 254, 317, 421, 2297, 3091
BspCNI CTCAG 6 cut(s) 229, 438, 641, 1510, 2452, 2914
BspDI ATCGAT 2 cut(s) 2090, 2834
BspFNI CGCG 5 cut(s) 125, 498, 1975, 2371, 3122
BspHI TCATGA 1 cut(s) 189
BspLI GGNNCC 6 cut(s) 210, 972, 1069, 1659, 1905, 2478
BspMAI CTGCAG 1 cut(s) 153
BspMI ACCTGC 1 cut(s) 3149
BspPI GGATC 6 cut(s) 1574, 1781, 2035, 2168, 2640, 2839
BspQI GCTCTTC 1 cut(s) 2538
BspT104I TTCGAA 1 cut(s) 2528
BspT107I GGYRCC 1 cut(s) 1903
BspTNI GGTCTC 1 cut(s) 830
BsrDI GCAATG 4 cut(s) 664, 1975, 2601, 2984
BsrFI RCCGGY 2 cut(s) 323, 787
BsrI ACTGG 7 cut(s) 550, 1394, 1510, 2426, 2451, 2463, 2505
BssAI RCCGGY 2 cut(s) 323, 787
BssNI GRCGYC 1 cut(s) 216
BssT1I CCWWGG 5 cut(s) 178, 255, 598, 1894, 2471
Bst2UI CCWGG 6 cut(s) 213, 418, 1641, 1851, 2262, 3151
Bst6I CTCTTC 3 cut(s) 124, 2119, 2538
BstACI GRCGYC 1 cut(s) 216
BstAPI GCANNNNNTGC 1 cut(s) 385
BstBI TTCGAA 1 cut(s) 2528
BstC8I GCNNGC 8 cut(s) 149, 325, 468, 496, 1320, 2618, 2622, 3241
BstDEI CTNAG 9 cut(s) 237, 446, 628, 1518, 2059, 2120, 2439, 2520, 2922
BstDSI CCRYGG 2 cut(s) 255, 1894
BstEII GGTNACC 1 cut(s) 1457
BstF5I GGATG 3 cut(s) 955, 1267, 1453
BstFNI CGCG 5 cut(s) 125, 498, 1975, 2371, 3122
BstH2I RGCGCY 1 cut(s) 473
BstHHI GCGC 8 cut(s) 127, 472, 500, 1324, 1885, 1975, 2719, 2728
BstMAI GTCTC 6 cut(s) 121, 277, 788, 830, 1011, 1582
BstMCI CGRYCG 1 cut(s) 3120
BstNI CCWGG 6 cut(s) 213, 418, 1641, 1851, 2262, 3151
BstNSI RCATGY 2 cut(s) 1322, 3243
BstPI GGTNACC 1 cut(s) 1457
BstSCI CCNGG 8 cut(s) 211, 416, 1639, 1849, 2180, 2260, 3023, 3149
BstSFI CTRYAG 4 cut(s) 149, 651, 1001, 2376
BstSLI GKGCMC 1 cut(s) 1344
BstUI CGCG 5 cut(s) 125, 498, 1975, 2371, 3122
BstV2I GAAGAC 2 cut(s) 432, 2541
BstX2I RGATCY 4 cut(s) 814, 1304, 1399, 1786
BstXI CCANNNNNNTGG 2 cut(s) 205, 1351
BstYI RGATCY 4 cut(s) 814, 1304, 1399, 1786
Bsu15I ATCGAT 2 cut(s) 2090, 2834
Bsu36I CCTNAGG 1 cut(s) 2922
BsuRI GGCC 6 cut(s) 147, 254, 317, 421, 2297, 3091
BsuTUI ATCGAT 2 cut(s) 2090, 2834
BtgI CCRYGG 2 cut(s) 255, 1894
BtgZI GCGATG 1 cut(s) 602
BtsCI GGATG 3 cut(s) 955, 1267, 1453
BtsIMutI CAGTG 4 cut(s) 1503, 1818, 2470, 2512
BtuMI TCGCGA 1 cut(s) 3122
BveI ACCTGC 1 cut(s) 3149
Cac8I GCNNGC 8 cut(s) 149, 325, 468, 496, 1320, 2618, 2622, 3241
CaiI CAGNNNCTG 3 cut(s) 385, 712, 2446
CciI TCATGA 1 cut(s) 189
CfoI GCGC 8 cut(s) 127, 472, 500, 1324, 1885, 1975, 2719, 2728
Cfr10I RCCGGY 2 cut(s) 323, 787
Cfr13I GGNCC 3 cut(s) 971, 1067, 2366
ClaI ATCGAT 2 cut(s) 2090, 2834
CseI GACGC 3 cut(s) 205, 377, 401
Csp6I GTAC 5 cut(s) 70, 1977, 2857, 2962, 3058
CspCI CAANNNNNGTGG 2 cut(s) 1153, 1188
CviQI GTAC 5 cut(s) 70, 1977, 2857, 2962, 3058
DdeI CTNAG 9 cut(s) 237, 446, 628, 1518, 2059, 2120, 2439, 2520, 2922
DraI TTTAAA 1 cut(s) 2581
DrdI GACNNNNNNGTC 1 cut(s) 2915
DseDI GACNNNNNNGTC 1 cut(s) 2915
EaeI YGGCCR 3 cut(s) 252, 315, 419
Eam1104I CTCTTC 3 cut(s) 124, 2119, 2538
EarI CTCTTC 3 cut(s) 124, 2119, 2538
EciI GGCGGA 2 cut(s) 275, 806
Ecl136II GAGCTC 2 cut(s) 195, 478
Eco130I CCWWGG 5 cut(s) 178, 255, 598, 1894, 2471
Eco24I GRGCYC 2 cut(s) 197, 480
Eco31I GGTCTC 1 cut(s) 830
Eco47I GGWCC 3 cut(s) 971, 1067, 2366
Eco53kI GAGCTC 2 cut(s) 195, 478
Eco57I CTGAAG 1 cut(s) 2472
Eco81I CCTNAGG 1 cut(s) 2922
Eco88I CYCGRG 2 cut(s) 473, 2646
Eco91I GGTNACC 1 cut(s) 1457
EcoICRI GAGCTC 2 cut(s) 195, 478
EcoO109I RGGNCCY 1 cut(s) 1067
EcoO65I GGTNACC 1 cut(s) 1457
EcoRI GAATTC 2 cut(s) 52, 695
EcoRII CCWGG 6 cut(s) 211, 416, 1639, 1849, 2260, 3149
EcoT14I CCWWGG 5 cut(s) 178, 255, 598, 1894, 2471
EcoT22I ATGCAT 4 cut(s) 828, 1320, 1822, 3241
EcoT38I GRGCYC 2 cut(s) 197, 480
ErhI CCWWGG 5 cut(s) 178, 255, 598, 1894, 2471
Esp3I CGTCTC 2 cut(s) 277, 1011
FalI AAGNNNNNCTT 2 cut(s) 1113, 1145
FaqI GGGAC 6 cut(s) 174, 399, 1053, 1075, 2179, 2903
FauI CCCGC 2 cut(s) 503, 3268
FbaI TGATCA 2 cut(s) 2934, 3222
FokI GGATG 3 cut(s) 962, 1274, 1460
FriOI GRGCYC 2 cut(s) 197, 480
FspAI RTGCGCAY 1 cut(s) 1323
FspBI CTAG 8 cut(s) 179, 1335, 1356, 1647, 2189, 2540, 2574, 2720
FspI TGCGCA 1 cut(s) 1323
GlaI GCGC 8 cut(s) 126, 471, 499, 1323, 1884, 1974, 2718, 2727
GsuI CTGGAG 2 cut(s) 750, 2468
HaeII RGCGCY 1 cut(s) 473
HaeIII GGCC 6 cut(s) 147, 254, 317, 421, 2297, 3091
HapII CCGG 6 cut(s) 324, 762, 788, 2181, 2480, 3024
HgaI GACGC 3 cut(s) 205, 377, 401
HhaI GCGC 8 cut(s) 127, 472, 500, 1324, 1885, 1975, 2719, 2728
Hin1I GRCGYC 1 cut(s) 216
Hin6I GCGC 8 cut(s) 125, 470, 498, 1322, 1883, 1973, 2717, 2726
HinP1I GCGC 8 cut(s) 125, 470, 498, 1322, 1883, 1973, 2717, 2726
HincII GTYRAC 3 cut(s) 1278, 1726, 1999
HindII GTYRAC 3 cut(s) 1278, 1726, 1999
HindIII AAGCTT 3 cut(s) 678, 852, 2522
HpaI GTTAAC 2 cut(s) 1278, 1999
HpaII CCGG 6 cut(s) 324, 762, 788, 2181, 2480, 3024
HphI GGTGA 9 cut(s) 1200, 2018, 2423, 2689, 2860, 3077, 3110, 3245, 3249
Hpy166II GTNNAC 6 cut(s) 759, 1278, 1726, 1999, 2468, 2851
Hpy8I GTNNAC 6 cut(s) 759, 1278, 1726, 1999, 2468, 2851
Hpy99I CGWCG 3 cut(s) 393, 434, 1146
HpyAV CCTTC 8 cut(s) 151, 579, 1115, 2287, 2302, 2728, 2761, 3265
HpyCH4IV ACGT 2 cut(s) 23, 432
HpyF3I CTNAG 9 cut(s) 237, 446, 628, 1518, 2059, 2120, 2439, 2520, 2922
HpySE526I ACGT 2 cut(s) 23, 432
Hsp92I GRCGYC 1 cut(s) 216
HspAI GCGC 8 cut(s) 125, 470, 498, 1322, 1883, 1973, 2717, 2726
KroI GCCGGC 1 cut(s) 323
KroNI GCCGGC 1 cut(s) 325
Ksp22I TGATCA 2 cut(s) 2934, 3222
KspAI GTTAAC 2 cut(s) 1278, 1999
LguI GCTCTTC 1 cut(s) 2538
LmnI GCTCC 5 cut(s) 200, 376, 483, 629, 2497
LweI GCATC 5 cut(s) 1234, 1939, 2704, 2713, 3226
MaeI CTAG 8 cut(s) 179, 1335, 1356, 1647, 2189, 2540, 2574, 2720
MaeII ACGT 2 cut(s) 23, 432
MaeIII GTNAC 7 cut(s) 224, 604, 1096, 1457, 2969, 3137, 3255
MfeI CAATTG 1 cut(s) 1251
MflI RGATCY 4 cut(s) 814, 1304, 1399, 1786
MhlI GDGCHC 4 cut(s) 197, 480, 1344, 3086
MlsI TGGCCA 2 cut(s) 254, 421
MluNI TGGCCA 2 cut(s) 254, 421
MlyI GAGTC 4 cut(s) 156, 508, 601, 1232
MmeI TCCRAC 5 cut(s) 788, 1705, 1813, 2201, 2308
Mox20I TGGCCA 2 cut(s) 254, 421
Mph1103I ATGCAT 4 cut(s) 828, 1320, 1822, 3241
MreI CGCCGGCG 1 cut(s) 323
MroNI GCCGGC 1 cut(s) 323
MroXI GAANNNNTTC 2 cut(s) 23, 1125
MscI TGGCCA 2 cut(s) 254, 421
MseI TTAA 9 cut(s) 510, 1031, 1277, 1773, 1877, 1998, 2096, 2580, 2889
MslI CAYNNNNRTG 4 cut(s) 936, 1349, 1497, 2303
Msp20I TGGCCA 2 cut(s) 254, 421
MspA1I CMGCKG 7 cut(s) 382, 632, 1409, 2171, 2223, 2609, 3263
MspI CCGG 6 cut(s) 324, 762, 788, 2181, 2480, 3024
MspR9I CCNGG 8 cut(s) 213, 418, 1641, 1851, 2182, 2262, 3025, 3151
MunI CAATTG 1 cut(s) 1251
Mva1269I GAATGC 2 cut(s) 771, 1822
MvaI CCWGG 6 cut(s) 213, 418, 1641, 1851, 2262, 3151
MvnI CGCG 5 cut(s) 125, 498, 1975, 2371, 3122
NaeI GCCGGC 1 cut(s) 325
NciI CCSGG 2 cut(s) 2182, 3025
NcoI CCATGG 2 cut(s) 255, 1894
NgoMIV GCCGGC 1 cut(s) 323
NlaIV GGNNCC 6 cut(s) 210, 972, 1069, 1659, 1905, 2478
NmeAIII GCCGAG 1 cut(s) 2960
NmuCI GTSAC 3 cut(s) 604, 2969, 3255
NruI TCGCGA 1 cut(s) 3122
NsbI TGCGCA 1 cut(s) 1323
NsiI ATGCAT 4 cut(s) 828, 1320, 1822, 3241
NspI RCATGY 2 cut(s) 1322, 3243
NspV TTCGAA 1 cut(s) 2528
OliI CACNNNNGTG 1 cut(s) 2303
PaeI GCATGC 2 cut(s) 1322, 3243
PaeR7I CTCGAG 1 cut(s) 473
PagI TCATGA 1 cut(s) 189
PaqCI CACCTGC 1 cut(s) 3149
PciSI GCTCTTC 1 cut(s) 2538
PctI GAATGC 2 cut(s) 771, 1822
PdiI GCCGGC 1 cut(s) 325
PdmI GAANNNNTTC 2 cut(s) 23, 1125
PflMI CCANNNNNTGG 1 cut(s) 846
Ple19I CGATCG 1 cut(s) 3120
PleI GAGTC 4 cut(s) 156, 508, 601, 1232
PpsI GAGTC 4 cut(s) 156, 508, 601, 1232
PpuMI RGGWCCY 1 cut(s) 1067
PshBI ATTAAT 1 cut(s) 2096
Psp124BI GAGCTC 2 cut(s) 197, 480
Psp1406I AACGTT 1 cut(s) 23
Psp5II RGGWCCY 1 cut(s) 1067
Psp6I CCWGG 6 cut(s) 211, 416, 1639, 1849, 2260, 3149
PspEI GGTNACC 1 cut(s) 1457
PspGI CCWGG 6 cut(s) 211, 416, 1639, 1849, 2260, 3149
PspN4I GGNNCC 6 cut(s) 210, 972, 1069, 1659, 1905, 2478
PspPI GGNCC 3 cut(s) 971, 1067, 2366
PspPPI RGGWCCY 1 cut(s) 1067
PspXI VCTCGAGB 1 cut(s) 473
PstI CTGCAG 1 cut(s) 153
PstNI CAGNNNCTG 3 cut(s) 385, 712, 2446
PsuI RGATCY 4 cut(s) 814, 1304, 1399, 1786
PvuI CGATCG 1 cut(s) 3120
PvuII CAGCTG 5 cut(s) 382, 632, 1409, 2171, 2609
RgaI GCGATCGC 1 cut(s) 3120
RruI TCGCGA 1 cut(s) 3122
RsaI GTAC 5 cut(s) 71, 1978, 2858, 2963, 3059
RsaNI GTAC 5 cut(s) 70, 1977, 2857, 2962, 3058
RseI CAYNNNNRTG 4 cut(s) 936, 1349, 1497, 2303
SacI GAGCTC 2 cut(s) 197, 480
SapI GCTCTTC 1 cut(s) 2538
SaqAI TTAA 9 cut(s) 510, 1031, 1277, 1773, 1877, 1998, 2096, 2580, 2889
Sau96I GGNCC 3 cut(s) 971, 1067, 2366
SbfI CCTGCAGG 1 cut(s) 153
SchI GAGTC 4 cut(s) 156, 508, 601, 1232
ScrFI CCNGG 8 cut(s) 213, 418, 1641, 1851, 2182, 2262, 3025, 3151
SdaI CCTGCAGG 1 cut(s) 153
SduI GDGCHC 4 cut(s) 197, 480, 1344, 3086
SfaAI GCGATCGC 1 cut(s) 3120
SfaNI GCATC 5 cut(s) 1234, 1939, 2704, 2713, 3226
SfcI CTRYAG 4 cut(s) 149, 651, 1001, 2376
Sfr274I CTCGAG 1 cut(s) 473
SfuI TTCGAA 1 cut(s) 2528
SgfI GCGATCGC 1 cut(s) 3120
SgrAI CRCCGGYG 1 cut(s) 323
SinI GGWCC 3 cut(s) 971, 1067, 2366
SlaI CTCGAG 1 cut(s) 473
SmiMI CAYNNNNRTG 4 cut(s) 936, 1349, 1497, 2303
SmlI CTYRAG 2 cut(s) 473, 1931
SmoI CTYRAG 2 cut(s) 473, 1931
SphI GCATGC 2 cut(s) 1322, 3243
Sse8387I CCTGCAGG 1 cut(s) 153
SspI AATATT 3 cut(s) 1439, 1916, 3105
SspMI CTAG 8 cut(s) 179, 1335, 1356, 1647, 2189, 2540, 2574, 2720
SstI GAGCTC 2 cut(s) 197, 480
StyD4I CCNGG 8 cut(s) 211, 416, 1639, 1849, 2180, 2260, 3023, 3149
StyI CCWWGG 5 cut(s) 178, 255, 598, 1894, 2471
TaiI ACGT 2 cut(s) 26, 435
TatI WGTACW 1 cut(s) 2961
TauI GCSGC 3 cut(s) 320, 323, 367
Tru1I TTAA 9 cut(s) 510, 1031, 1277, 1773, 1877, 1998, 2096, 2580, 2889
Tru9I TTAA 9 cut(s) 510, 1031, 1277, 1773, 1877, 1998, 2096, 2580, 2889
TscAI CASTG 4 cut(s) 1510, 1818, 2470, 2512
TseFI GTSAC 3 cut(s) 604, 2969, 3255
Tsp45I GTSAC 3 cut(s) 604, 2969, 3255
TspGWI ACGGA 5 cut(s) 294, 473, 473, 1220, 1579
TspRI CASTG 4 cut(s) 1510, 1818, 2470, 2512
Van91I CCANNNNNTGG 1 cut(s) 846
VpaK11BI GGWCC 3 cut(s) 971, 1067, 2366
VspI ATTAAT 1 cut(s) 2096
XbaI TCTAGA 2 cut(s) 1646, 2188
XceI RCATGY 2 cut(s) 1322, 3243
XhoI CTCGAG 1 cut(s) 473
XmaJI CCTAGG 1 cut(s) 178
XmnI GAANNNNTTC 2 cut(s) 23, 1125
XspI CTAG 8 cut(s) 179, 1335, 1356, 1647, 2189, 2540, 2574, 2720
Zsp2I ATGCAT 4 cut(s) 828, 1320, 1822, 3241
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.