Rroxscaffold_2G00149790
ERF Family

Probably involved in the RNA silencing pathway and required for the generation of small interfering RNAs (siRNAs)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000505
Physical Location & Seq
Reverse (-)
87281362 .. 87291776
10415 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00149790.1

Sequence Viewer

Length: 1869 bp
ATGGTAAAGTTCGAGACCACAGTTTGGCATGCTTTGGGTGAAAACAATGCTGGCAAGGCTGACCGACGATTGTATTCTGATTGGGATTCTCAAAGAACACACCAGTACTATTGTTATGTTTCCGTGGATGGGAGTTACAAGTTCAAGGGTCCCTGCCTAGACAAACCAAGAACTCTCCTACAGAAACAGTTAGGAGATGACAATGTTTTGGAGGTGAAATTTACCCAAGGGGAAGGTTCCAGTGGTACATATAGCAAGTTAATGCGAGAAGGCATTCTTGTTGGTTTGCGGCGATATGCTTTCTTTGTGTTCCGTGCTGGTAACAAAGGAAAGAATATGTGGAAAGACCCAACTTCATCACCTGTGAAATGTTATTTTGTTCGTGGGAGCTCTGATGCAGTGATAGACAGGGGTATAGGCTATAAGTTATCTGGCAAAACGATGCATGAAGGAAGATCTCTTTTCACGCATGCACACACTCGGCCTAGTTTGGCCAGCTACATGGATAGGTTATCTCTCATTTTGTCCAAGACAGTTACTTTAGAAGTAAATTGGTCACATGTCAAGGTGGAATTCATCAAAGACAAAGACTGTCGCGATGAGTCTGGTAATCCCATCTATGTAGATGGAAAACCTCGCATACATACTGATGGCACAGGATTTATATCTGAGGATCTGGCTTTACTTTGTCCTGAGATAGCACGCAAAGGAGAGCAGATCAATAACGAACACATCGAGGGACTTGATGATCCTGATGATGAACTTGAGGACAAAATTCTGGAATGGAAACAGCCAGGCACTAGAACTCGGGAACTGCCTTTGCTCATGCAGTTCCGACTTTTTTACGAAGGCCGTGCTATCAAAGGAACTTTTCTTGTCAATAAAGAGCTTCCTCCAAAAACTTTGAAGATTCGACCATCAATGGTTAAAGTTGAGCCAGATCCAAAATTGTCAGATCCTCAAACAGTGAATTCACTGGAGATAGTAGGAACAAGTAAACTTAATAAGAGAACGTATTTGTCGAGGAACTTAATTGCACTTTTGTACCATGGAGGGGTGCCAAAAAAATACTTCATCGAGTTACTTGTGAAAGCTTTAGAAGAGACTCGTGATGTTTTTAGCAATCCACGCGCAGCATTCAGAGTTGCCCTTAGATATGGCGAGATCGATGACAACAGTACTGTTGCAAAAATGATCTCCTGTGGTATTCCTCTTGAAGAACCATATGTGCAATATCGTCTTTCAACCCTTATGAACATGGAAAAGATCAATCTCAAAAAAGGGAAAATTTGTATTCCTGAATCATACTATTTGATGGGGACAGCTGATCCAACAGGAAAACTTGAAAAAGATGAAGTTTGCATTATCCTAGAAAATGGACAACTACTGTCGGGAGATTTTCTAGTGTACCGGAATCCTGGTTTACACTTCGGTGATATTCATGTCCTAAAGGCCAGATATGTCAAGGAACTAGAATCTGTTGTTGGCAATGCCAAGTACGGTATATTCTTCTCTAGTAAAGGTCCACGTTCCGTTGCTGATGAAATGGCTGGTGGAGATTTTGATGGTGATCTATACTGGATCTCAAGAAACCCTCAGCTATTAAAATCGTTTAAACCAAGTGAACCTTGGATTGGAAAGTCATCTGCCCAAAAAGTTCATGGTAAAAGACCAACCGAGCTTTCAAAGGATGATCTAGAAGATGAGCTCATCAAACTATTCTTGAAGACTAGGTTTGAACCAAGCTTCGCAGTGTATGAGGCAGCTCAAAACTGGATGGCTTTGATGGATAAATTTTTGACTCTAGGAGATACGAGTATTGATGTGAAAAACAAACTGAAGGATAAGATCGATATTGCGATTGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RdRP

Protein Analysis

622

Amino Acids

70.59

Weight (kDa)

8.46

Isoelectric Point (pI)

25.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RdRP PF05183 47 - 598 1.1e-86 RNA dependent RNA polymerase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000505)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G19910 AT2G19910 AT2G19920 AT2G19920 AT2G19920 AT2G19920 AT2G19930 AT2G19930 AT2G19930
fragaria_vesca FvH4_1g05980 FvH4_1g05990 FvH4_1g05990 FvH4_1g09718
malus_domestica MD02G1064400.v1.1 MD15G1195300.v1.1 MD15G1195400.v1.1
prunus_persica Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1
pyrus_communis pycom02g05070 pycom15g17460
rosa_chinensis RchiOBHm_Chr2g0091671 RchiOBHm_Chr2g0091681 RchiOBHm_Chr2g0091691 RchiOBHm_Chr2g0091701 RchiOBHm_Chr2g0091711 RchiOBHm_Chr2g0091721 RchiOBHm_Chr2g0091731 RchiOBHm_Chr5g0014401
rosa_laevigata RLG00000013772 RLG00000016242 RLG00000016244 RLG00000016245 RLG00000016246 RLG00000032105
rosa_multiflora Rmu_co8374041.1_g000001 Rmu_co8478121.1_g000001 Rmu_sc0000897.1_g000005 Rmu_sc0000897.1_g000009
rosa_roxburghii Rroxscaffold_1G00062180 Rroxscaffold_1G00062190 Rroxscaffold_1G00068800 Rroxscaffold_2G00149790 Rroxscaffold_2G00149800 Rroxscaffold_2G00149810
rosa_rugosa Rorug02G0019600 Rorug02G0019700 Rorug02G0019800 Rorug02G0019900 Rorug02G0019900 Rorug02G0020000 Rorug02G0020000 Rorug02G0020100 Rorug02G0020100 Rorug02G0020200 Rorug05G0017800
rosa_samantha Rh2AG064700 Rh2AG064800 Rh2AG064900 Rh2BG063800 Rh2BG063900 Rh2BG064000 Rh2BG064100 Rh2CG065500 Rh2CG065600 Rh2CG065700 Rh2DG063900 Rh2DG064000 Rh2DG064100 Rh2DG064200 Rh2DG381700 Rh5AG056700 Rh5AG056800 Rh5AG056900 Rh5AG112600 Rh5BG109300 Rh5CG063800 Rh5CG121100 Rh5DG053100 Rh5DG108300 Rh6CG100100
rosa_wichuraiana Rw2G005560 Rw2G005580 Rw2G005590 Rw5G005370 Rw5G009770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1057
AccB7I CCANNNNNTGG 1 cut(s) 24
AccII CGCG 2 cut(s) 597, 1131
AciI CCGC 1 cut(s) 289
AclWI GGATC 6 cut(s) 681, 743, 935, 950, 1322, 1589
AcoI YGGCCR 1 cut(s) 492
AcsI RAATTY 6 cut(s) 218, 572, 774, 970, 1287, 1793
AcuI CTGAAG 1 cut(s) 1859
AfaI GTAC 6 cut(s) 107, 247, 1046, 1180, 1409, 1499
AfiI CCNNNNNNNGG 4 cut(s) 24, 129, 1054, 1634
AflIII ACRYGT 1 cut(s) 559
AgsI TTSAA 8 cut(s) 145, 907, 1217, 1245, 1346, 1686, 1726, 1739
AjnI CCWGG 2 cut(s) 793, 1417
AjuI GAANNNNNNNTTGG 6 cut(s) 1467, 1499, 1617, 1649, 1666, 1698
AleI CACNNNNGTG 1 cut(s) 1431
Alw21I GWGCWC 2 cut(s) 392, 1710
Alw26I GTCTC 2 cut(s) 8, 1097
AlwI GGATC 6 cut(s) 681, 743, 935, 950, 1322, 1589
Ama87I CYCGRG 1 cut(s) 807
AoxI GGCC 4 cut(s) 482, 492, 850, 1452
ApeKI GCWGC 2 cut(s) 1133, 1763
ApoI RAATTY 6 cut(s) 218, 572, 774, 970, 1287, 1793
Asp700I GAANNNNTTC 1 cut(s) 273
AspLEI GCGC 1 cut(s) 1133
AspS9I GGNCC 2 cut(s) 149, 1523
AsuHPI GGTGA 5 cut(s) 50, 226, 351, 1445, 1580
AvaI CYCGRG 1 cut(s) 807
AvaII GGWCC 2 cut(s) 149, 1523
BaeI ACNNNNGTAYC 2 cut(s) 1028, 1061
BalI TGGCCA 1 cut(s) 494
BanI GGYRCC 1 cut(s) 1057
BanII GRGCYC 2 cut(s) 392, 1710
BauI CACGAG 1 cut(s) 1107
BbsI GAAGAC 1 cut(s) 1733
Bbv12I GWGCWC 2 cut(s) 392, 1710
BbvCI CCTCAGC 1 cut(s) 1596
BbvI GCAGC 2 cut(s) 1145, 1775
BccI CCATC 9 cut(s) 122, 620, 623, 644, 925, 1309, 1559, 1771, 1780
BceAI ACGGC 1 cut(s) 837
BcgI CGANNNNNNTGC 2 cut(s) 836, 870
BciT130I CCWGG 2 cut(s) 795, 1419
BcoDI GTCTC 2 cut(s) 8, 1097
BfmI CTRYAG 1 cut(s) 179
BglII AGATCT 1 cut(s) 455
BisI GCNGC 3 cut(s) 290, 1134, 1764
BlsI GCNGC 3 cut(s) 291, 1135, 1765
BmcAI AGTACT 2 cut(s) 107, 1180
Bme1390I CCNGG 2 cut(s) 795, 1419
Bme18I GGWCC 2 cut(s) 149, 1523
BmeT110I CYCGRG 1 cut(s) 807
BmgT120I GGNCC 2 cut(s) 149, 1523
BmiI GGNNCC 4 cut(s) 150, 151, 238, 1059
BmrFI CCNGG 2 cut(s) 795, 1419
BmsI GCATC 2 cut(s) 385, 432
BpiI GAAGAC 1 cut(s) 1733
BpmI CTGGAG 1 cut(s) 998
Bpu10I CCTNAGC 1 cut(s) 1596
BpuEI CTTGAG 2 cut(s) 785, 1570
Bsa29I ATCGAT 2 cut(s) 1167, 1851
BsaBI GATNNNNATC 2 cut(s) 664, 1569
BsaI GGTCTC 1 cut(s) 8
BsaJI CCNNGG 4 cut(s) 123, 226, 1048, 1628
BsaWI WCCGGW 1 cut(s) 1410
Bsc4I CCNNNNNNNGG 4 cut(s) 24, 129, 1054, 1634
Bse1I ACTGG 5 cut(s) 103, 240, 981, 1583, 1778
Bse3DI GCAATG 1 cut(s) 1495
Bse8I GATNNNNATC 2 cut(s) 664, 1569
BseBI CCWGG 2 cut(s) 795, 1419
BseCI ATCGAT 2 cut(s) 1167, 1851
BseDI CCNNGG 4 cut(s) 123, 226, 1048, 1628
BseGI GGATG 3 cut(s) 133, 1696, 1782
BseJI GATNNNNATC 2 cut(s) 664, 1569
BseLI CCNNNNNNNGG 4 cut(s) 24, 129, 1054, 1634
BseMI GCAATG 1 cut(s) 1495
BseMII CTCAG 3 cut(s) 660, 684, 1610
BseNI ACTGG 5 cut(s) 103, 240, 981, 1583, 1778
BseXI GCAGC 2 cut(s) 1145, 1775
Bsh1236I CGCG 2 cut(s) 597, 1131
BshFI GGCC 4 cut(s) 484, 494, 852, 1454
BshNI GGYRCC 1 cut(s) 1057
BshVI ATCGAT 2 cut(s) 1167, 1851
BsiHKAI GWGCWC 2 cut(s) 392, 1710
BsiHKCI CYCGRG 1 cut(s) 807
BsiSI CCGG 1 cut(s) 1411
BslFI GGGAC 3 cut(s) 135, 753, 1333
BslI CCNNNNNNNGG 4 cut(s) 24, 129, 1054, 1634
BsmAI GTCTC 2 cut(s) 8, 1097
BsmFI GGGAC 3 cut(s) 135, 753, 1333
BsmI GAATGC 2 cut(s) 273, 1136
BsnI GGCC 4 cut(s) 484, 494, 852, 1454
Bso31I GGTCTC 1 cut(s) 8
BsoBI CYCGRG 1 cut(s) 807
Bsp1286I GDGCHC 2 cut(s) 392, 1710
Bsp19I CCATGG 1 cut(s) 1048
Bsp68I TCGCGA 1 cut(s) 597
BspACI CCGC 1 cut(s) 289
BspANI GGCC 4 cut(s) 484, 494, 852, 1454
BspCNI CTCAG 3 cut(s) 661, 685, 1609
BspDI ATCGAT 2 cut(s) 1167, 1851
BspFNI CGCG 2 cut(s) 597, 1131
BspLI GGNNCC 4 cut(s) 150, 151, 238, 1059
BspPI GGATC 6 cut(s) 681, 743, 935, 950, 1322, 1589
BspT107I GGYRCC 1 cut(s) 1057
BspTNI GGTCTC 1 cut(s) 8
BsrDI GCAATG 1 cut(s) 1495
BsrI ACTGG 5 cut(s) 103, 240, 981, 1583, 1778
BssECI CCNNGG 4 cut(s) 123, 226, 1048, 1628
BssSI CACGAG 1 cut(s) 1107
BssT1I CCWWGG 3 cut(s) 226, 1048, 1628
Bst2BI CACGAG 1 cut(s) 1107
Bst2UI CCWGG 2 cut(s) 795, 1419
Bst4CI ACNGT 9 cut(s) 22, 189, 535, 593, 967, 1178, 1183, 1389, 1502
Bst6I CTCTTC 1 cut(s) 1095
BstC8I GCNNGC 5 cut(s) 30, 52, 471, 496, 703
BstDEI CTNAG 4 cut(s) 669, 693, 1151, 1596
BstDSI CCRYGG 2 cut(s) 123, 1048
BstF5I GGATG 3 cut(s) 133, 1696, 1782
BstFNI CGCG 2 cut(s) 597, 1131
BstHHI GCGC 1 cut(s) 1133
BstMAI GTCTC 2 cut(s) 8, 1097
BstMWI GCNNNNNNNGC 2 cut(s) 56, 1128
BstNI CCWGG 2 cut(s) 795, 1419
BstNSI RCATGY 3 cut(s) 32, 473, 563
BstSCI CCNGG 2 cut(s) 793, 1417
BstSFI CTRYAG 1 cut(s) 179
BstUI CGCG 2 cut(s) 597, 1131
BstV1I GCAGC 2 cut(s) 1145, 1775
BstV2I GAAGAC 1 cut(s) 1733
BstX2I RGATCY 5 cut(s) 455, 673, 940, 955, 1581
BstXI CCANNNNNNTGG 1 cut(s) 502
BstYI RGATCY 5 cut(s) 455, 673, 940, 955, 1581
Bsu15I ATCGAT 2 cut(s) 1167, 1851
BsuRI GGCC 4 cut(s) 484, 494, 852, 1454
BsuTUI ATCGAT 2 cut(s) 1167, 1851
BtgI CCRYGG 2 cut(s) 123, 1048
BtgZI GCGATG 1 cut(s) 612
BtsCI GGATG 3 cut(s) 133, 1696, 1782
BtsI GCAGTG 2 cut(s) 405, 1758
BtsIMutI CAGTG 5 cut(s) 247, 405, 972, 974, 1758
BtuMI TCGCGA 1 cut(s) 597
Cac8I GCNNGC 5 cut(s) 30, 52, 471, 496, 703
CfoI GCGC 1 cut(s) 1133
Cfr13I GGNCC 2 cut(s) 149, 1523
ClaI ATCGAT 2 cut(s) 1167, 1851
Csp6I GTAC 6 cut(s) 106, 246, 1045, 1179, 1408, 1498
CviQI GTAC 6 cut(s) 106, 246, 1045, 1179, 1408, 1498
DdeI CTNAG 4 cut(s) 669, 693, 1151, 1596
DraI TTTAAA 1 cut(s) 1615
EaeI YGGCCR 1 cut(s) 492
Eam1104I CTCTTC 1 cut(s) 1095
EarI CTCTTC 1 cut(s) 1095
Ecl136II GAGCTC 2 cut(s) 390, 1708
Eco130I CCWWGG 3 cut(s) 226, 1048, 1628
Eco24I GRGCYC 2 cut(s) 392, 1710
Eco31I GGTCTC 1 cut(s) 8
Eco47I GGWCC 2 cut(s) 149, 1523
Eco53kI GAGCTC 2 cut(s) 390, 1708
Eco57I CTGAAG 1 cut(s) 1859
Eco88I CYCGRG 1 cut(s) 807
EcoICRI GAGCTC 2 cut(s) 390, 1708
EcoO109I RGGNCCY 1 cut(s) 149
EcoRI GAATTC 2 cut(s) 572, 970
EcoRII CCWGG 2 cut(s) 793, 1417
EcoT14I CCWWGG 3 cut(s) 226, 1048, 1628
EcoT22I ATGCAT 1 cut(s) 447
EcoT38I GRGCYC 2 cut(s) 392, 1710
ErhI CCWWGG 3 cut(s) 226, 1048, 1628
FalI AAGNNNNNCTT 4 cut(s) 261, 293, 1612, 1644
FaqI GGGAC 3 cut(s) 135, 753, 1333
FauNDI CATATG 1 cut(s) 1225
Fnu4HI GCNGC 3 cut(s) 290, 1134, 1764
FokI GGATG 3 cut(s) 140, 1703, 1789
FriOI GRGCYC 2 cut(s) 392, 1710
Fsp4HI GCNGC 3 cut(s) 290, 1134, 1764
GlaI GCGC 1 cut(s) 1132
GluI GCNGC 3 cut(s) 290, 1134, 1764
GsuI CTGGAG 1 cut(s) 998
HaeIII GGCC 4 cut(s) 484, 494, 852, 1454
HapII CCGG 1 cut(s) 1411
HhaI GCGC 1 cut(s) 1133
Hin6I GCGC 1 cut(s) 1131
HinP1I GCGC 1 cut(s) 1131
HindIII AAGCTT 2 cut(s) 1092, 1744
HinfI GANTC 8 cut(s) 86, 602, 910, 1105, 1301, 1414, 1475, 1801
HpaII CCGG 1 cut(s) 1411
HphI GGTGA 5 cut(s) 50, 226, 351, 1445, 1580
Hpy166II GTNNAC 5 cut(s) 998, 1408, 1424, 1526, 1625
Hpy188I TCNGA 6 cut(s) 79, 394, 670, 836, 955, 1142
Hpy8I GTNNAC 5 cut(s) 998, 1408, 1424, 1526, 1625
Hpy99I CGWCG 1 cut(s) 69
HpyAV CCTTC 5 cut(s) 227, 263, 443, 842, 1834
HpyCH4III ACNGT 9 cut(s) 22, 189, 535, 593, 967, 1178, 1183, 1389, 1502
HpyCH4IV ACGT 2 cut(s) 1013, 1528
HpyCH4V TGCA 8 cut(s) 398, 445, 473, 829, 1037, 1187, 1231, 1362
HpyF10VI GCNNNNNNNGC 2 cut(s) 56, 1128
HpyF3I CTNAG 4 cut(s) 669, 693, 1151, 1596
HpySE526I ACGT 2 cut(s) 1013, 1528
HspAI GCGC 1 cut(s) 1131
KflI GGGWCCC 1 cut(s) 149
LmnI GCTCC 1 cut(s) 387
Lsp1109I GCAGC 2 cut(s) 1145, 1775
LweI GCATC 2 cut(s) 385, 432
MaeII ACGT 2 cut(s) 1013, 1528
MaeIII GTNAC 5 cut(s) 134, 320, 535, 555, 1080
MboII GAAGA 7 cut(s) 465, 919, 1112, 1229, 1501, 1712, 1738
MflI RGATCY 5 cut(s) 455, 673, 940, 955, 1581
MhlI GDGCHC 2 cut(s) 392, 1710
MlsI TGGCCA 1 cut(s) 494
MluCI AATT 9 cut(s) 218, 550, 572, 774, 947, 970, 1032, 1287, 1793
MluNI TGGCCA 1 cut(s) 494
MlyI GAGTC 3 cut(s) 611, 1099, 1795
MmeI TCCRAC 2 cut(s) 859, 1355
Mox20I TGGCCA 1 cut(s) 494
Mph1103I ATGCAT 1 cut(s) 447
MroXI GAANNNNTTC 1 cut(s) 273
MscI TGGCCA 1 cut(s) 494
MseI TTAA 6 cut(s) 260, 927, 1002, 1031, 1604, 1614
MslI CAYNNNNRTG 2 cut(s) 648, 1431
Msp20I TGGCCA 1 cut(s) 494
MspA1I CMGCKG 1 cut(s) 1325
MspI CCGG 1 cut(s) 1411
MspR9I CCNGG 2 cut(s) 795, 1419
MssI GTTTAAAC 1 cut(s) 1615
Mva1269I GAATGC 2 cut(s) 273, 1136
MvaI CCWGG 2 cut(s) 795, 1419
MvnI CGCG 2 cut(s) 597, 1131
MwoI GCNNNNNNNGC 2 cut(s) 56, 1128
NcoI CCATGG 1 cut(s) 1048
NdeI CATATG 1 cut(s) 1225
NlaIV GGNNCC 4 cut(s) 150, 151, 238, 1059
NmeAIII GCCGAG 1 cut(s) 460
NmuCI GTSAC 1 cut(s) 555
NruI TCGCGA 1 cut(s) 597
NsiI ATGCAT 1 cut(s) 447
NspI RCATGY 3 cut(s) 32, 473, 563
OliI CACNNNNGTG 1 cut(s) 1431
PaeI GCATGC 2 cut(s) 32, 473
PciI ACATGT 1 cut(s) 559
PcsI WCGNNNNNNNCGW 2 cut(s) 732, 1019
PctI GAATGC 2 cut(s) 273, 1136
PdmI GAANNNNTTC 1 cut(s) 273
PfeI GAWTC 5 cut(s) 86, 910, 1301, 1414, 1475
PflMI CCANNNNNTGG 1 cut(s) 24
PkrI GCNGC 3 cut(s) 291, 1135, 1765
PleI GAGTC 3 cut(s) 610, 1099, 1795
PmeI GTTTAAAC 1 cut(s) 1615
PpsI GAGTC 3 cut(s) 610, 1099, 1795
PpuMI RGGWCCY 1 cut(s) 149
PscI ACATGT 1 cut(s) 559
Psp124BI GAGCTC 2 cut(s) 392, 1710
Psp5II RGGWCCY 1 cut(s) 149
Psp6I CCWGG 2 cut(s) 793, 1417
PspGI CCWGG 2 cut(s) 793, 1417
PspN4I GGNNCC 4 cut(s) 150, 151, 238, 1059
PspPI GGNCC 2 cut(s) 149, 1523
PspPPI RGGWCCY 1 cut(s) 149
PsuI RGATCY 5 cut(s) 455, 673, 940, 955, 1581
PvuII CAGCTG 1 cut(s) 1325
RruI TCGCGA 1 cut(s) 597
RsaI GTAC 6 cut(s) 107, 247, 1046, 1180, 1409, 1499
RsaNI GTAC 6 cut(s) 106, 246, 1045, 1179, 1408, 1498
RseI CAYNNNNRTG 2 cut(s) 648, 1431
SacI GAGCTC 2 cut(s) 392, 1710
SaqAI TTAA 6 cut(s) 260, 927, 1002, 1031, 1604, 1614
SatI GCNGC 3 cut(s) 290, 1134, 1764
Sau96I GGNCC 2 cut(s) 149, 1523
ScaI AGTACT 2 cut(s) 107, 1180
SchI GAGTC 3 cut(s) 611, 1099, 1795
ScrFI CCNGG 2 cut(s) 795, 1419
SduI GDGCHC 2 cut(s) 392, 1710
SfaNI GCATC 2 cut(s) 385, 432
SfcI CTRYAG 1 cut(s) 179
SinI GGWCC 2 cut(s) 149, 1523
SmiMI CAYNNNNRTG 2 cut(s) 648, 1431
SmlI CTYRAG 2 cut(s) 764, 1585
SmoI CTYRAG 2 cut(s) 764, 1585
SphI GCATGC 2 cut(s) 32, 473
Sse9I AATT 9 cut(s) 218, 550, 572, 774, 947, 970, 1032, 1287, 1793
SsiI CCGC 1 cut(s) 289
SstI GAGCTC 2 cut(s) 392, 1710
StyD4I CCNGG 2 cut(s) 793, 1417
StyI CCWWGG 3 cut(s) 226, 1048, 1628
TaaI ACNGT 9 cut(s) 22, 189, 535, 593, 967, 1178, 1183, 1389, 1502
TaiI ACGT 2 cut(s) 1016, 1531
TaqI TCGA 7 cut(s) 12, 735, 913, 1022, 1077, 1167, 1851
TaqII GACCGA 1 cut(s) 78
TasI AATT 9 cut(s) 218, 550, 572, 774, 947, 970, 1032, 1287, 1793
TatI WGTACW 2 cut(s) 105, 1178
TauI GCSGC 1 cut(s) 292
TfiI GAWTC 5 cut(s) 86, 910, 1301, 1414, 1475
Tru1I TTAA 6 cut(s) 260, 927, 1002, 1031, 1604, 1614
Tru9I TTAA 6 cut(s) 260, 927, 1002, 1031, 1604, 1614
TscAI CASTG 5 cut(s) 247, 405, 972, 981, 1758
TseFI GTSAC 1 cut(s) 555
TseI GCWGC 2 cut(s) 1133, 1763
Tsp45I GTSAC 1 cut(s) 555
TspGWI ACGGA 3 cut(s) 112, 302, 1522
TspRI CASTG 5 cut(s) 247, 405, 972, 981, 1758
Van91I CCANNNNNTGG 1 cut(s) 24
VpaK11BI GGWCC 2 cut(s) 149, 1523
XapI RAATTY 6 cut(s) 218, 572, 774, 970, 1287, 1793
XbaI TCTAGA 1 cut(s) 1696
XceI RCATGY 3 cut(s) 32, 473, 563
XcmI CCANNNNNNNNNTGG 2 cut(s) 1626, 1658
XmnI GAANNNNTTC 1 cut(s) 273
ZrmI AGTACT 2 cut(s) 107, 1180
Zsp2I ATGCAT 1 cut(s) 447
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.