Rw2G005590
ERF Family

Probably involved in the RNA silencing pathway and required for the generation of small interfering RNAs (siRNAs)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000505
Physical Location & Seq
Reverse (-)
5282151 .. 5292444
10294 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G005590.1

Sequence Viewer

Length: 3150 bp
ATGGAAGAAGTGAGATTACCTCCGCCGGTGGAGGGGTTGCTCCGTAGAATCTGTACTGAGAAAAAGCTACCATGGCCGGACGCCGGGGTCCGGCGGGAGCTGGCCTCTATCAGCCAGGAACGTGCACATGAGGTCCTGGTTAAAGTCTTCAACAAACAAGATATCATAACTCTCGGCGGTTTCATCAAGCACTTGATCCGTAACCTCCCTGCTTCTCCTCCTCCTCCGACAGCTGTCTGCGCTTATTATGATCAAAGCCCCTCTGCTTGTCCAGTTACGCCACCTTGTTCTCAACTTCAGTACAGCTCCGCAGTTGCAGGTCAGGCACTCAGTGATCGGACCGAGCAGCCTGTGATTAATGGACGGTATGGACCATCACCATATTCTTCTGGTTGTAAAGCCATCAGCTCTTTCTCGTATCCAACAATATCGCTGGCTTGTAACATTGCTCCAGTGCAACTTGATCATAGGTTTCAAGGTGAGGTTGTAACCAAGTCGCCAGATCTAGAGAAATATGGAGGCCATGGACAAGCACACTTGGCAGCTCTTGGTACTCTTGAATTCAGAAAACAATTTCTGATCTTGAGCTACATTAGAGAGGAAAAGTTAAAGACTGTTATAGTCGCAGAGGATATTAAGCATTTGAAAGATTTGCCAATGGCAGAGTTTGAGTCCAAAGTTTGGCTACTCTTCGGACATAAATATATCAGCAATGGAGACCGACAGTTGAATTTCGATTGGGATGGCAGACGCACACATTTCTATGAGTGCCATGTTACCAGAGATGGGAGTTACACATTCAAGGGCCCCTATCTACAAAATGAAAGAAGAACTCTCCTACAGAAACTGTGTCAAGATGACAATGTTTTGATTGTGAAGTTTGATGAAGAAGGGACTCAGACAGGTTCTAGGACTTCTTATGATAATGACTATTCTACGTATATGAAGGTTGAGAGACAAGGGATTTTTGTTGGCTTGCGGCGCTATCGTTTCTTTGGTTTTAAAGATGGTGGTAAAAGAGAGAAAATCAGAAGTCCCACGTCATCACCCGTAAAATGTTATTTTGTTCGTGTGAGCTCTGATGCAGCAATAGACAGGGATGTAGGTTATTGGTTATCTAACAGAACAATTCATGAAGGAAGACGTCTAATTATGCATGTTGATACTCTGCCAAGTGTGGACATACTCTGCCAAGTGTTGCCACTCATTTTTTCTAAGACAACTACCTTGGAAGTAGATTGGTCACTTGTGAAAGTTGAAGAAATTGTTGATGTCAACTGTTGCGATGAATCTGGTAACTGTGTTTATAATGAAGATGGAAAACCTCTTCTACATACAGATGGAACTGGATTCATATCTGAGGATTTGGCTTTACTTTGTCCAAAGATAGAAGCCAAAAGAAAGTTTATGAGTGGGGAACACATGGAGGGACTTCCGGATCCTGATGAACTTGAGATTGAAATGAAAAAATCAGATCTCCGAACTCAAGAACCGCCATTGCTCATACAGTGCCGCCTATTTCACGAAGGCAATGCTTATAAAGGAACTTTGCTTGTCAATAAGCGGCTTCCCCCGAAAACACTGCAGATTCGACCGTCAATGCGTAAAGTTGAGAAGGACCCTAAGATTTCAGATAATGAAACCATCAATTCTTTGGAAATAGTTTGCACAAGTGCTCCTCCTAGGAAAGCTTATTTGTCAAGGATTATAATTGCACTTCTAGTGCATGGAGGTGTACCGAAAGACTTCTTTATGAAGATACTTGACAATGCTTTAGAAGATGCTCATCGTGTTATCGGGAAGACACGTGCAGCGCTCAAAGTTGCTGAGAAGTATGGTGAGATTGATGACAATTTCTTGGGAGCAACAATGATCAGACTTGGTATTCCTCTAGAAGAATCATATTTGCAATATCGTCTGTCACACTTAATGCGTGTGCAGGAAAGCGCTGGTCTTAGAAAAGGGAGACTTCATATTCCTGACTCTTATGTGTTGATGGGGACAGCTGATCCAACAGGGATTCTGGAAAGAGATGAAGTCTGCGTCATCCTTAAGGACGGACAAATGTCGGGGAAGGTACTTGTGTACCGGTATCCTGGTTTACACTTTGGGGATATTAAAGTTTTGAAGGCCACCTATGTGAAGGAGTTAGAATCCACTGTGGGAGATGCCAGATATGCTATATTCTTCTCTTGCAAGGGACCACGTAATGTTGCTGATGAAATTGGTGGTGGAGATTTTGATGGTGATCTTTACTGGGTCTCAAGAAACCCTCAGTTTTTATATTTAATCACATATTATTGGAATTATTTCAGCTATTGGAAACCAAGTGAACCTTGGAACGAAGGATCAATACCAACTCAAAAGGTTGCCAGTAATAGACCAACTGAGCTTTCCACTGAAGATCTTGAAGCTGATATGTTTAGATTGTTCTTGAAAAATAGGTTTCAACCAAGTTTTGCTATGAGTGAGGCAGCTAATAACTGGCTAGCTTGGATAGATTGGTTCTTGACTTTGGGAGATAGTAATGAGAAGAAGGACGTAATGGACAAGATACTGAGGTTGGTTGATATATACTACATCGCTCTAGATGCAGCGAAGAAAGGTGTAAAGGTTGAAGTTCCAAAAGATTTGAAGGTGTCACTTTTCCCTCATTACATGGACAAGAAAAAAGAAGAAGAAACTTACATTTCATCTTCGATTTTGGGAGAAATTTATGATGAAGTTGATAAATGTCAAGCAAAAATAATTCTTCTCAAAGAAATCAAGAAACTTCCTATTTTTGATATTAATCAAGTCCCCGAACTCTGCTTGGCCAAATGGAAGAAACGTTATGGACGGTACAGGACTGAAATGAGCTCGGCCATGGATGAGGATGGAGATGAGGCTGCCATTGTCATAAGAAAGTATAAAAAGAAGCTGTATAAGGCTGATGACTTTGAGCACAGTTCAAGGCCGATGGATGAAATACTGGACGAGGCACACGCTATATATCATGTATGTTATGACCATGCTATGGATAAAAGAGATGTGAAAAAATGTTCCTTTGCATGGAGAGTTGCAGGTAAAGCTCTGTGCGAGCTTCACGCTAGGAAAAAGGATGAGAAGTGGTTAAACATTGCCCCTTCTGTTTTGAAGGAGCTCTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RdRP

Protein Analysis

1049

Amino Acids

119.77

Weight (kDa)

7.1

Isoelectric Point (pI)

42.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
4HB_RdRP3_N PF26249 4 - 67 3e-08 RdRP3/4/5, N-terminal four helical bundle domain
RdRP_helical PF26252 179 - 243 2.2e-08 RNA dependent RNA polymerase, helical domain
RdRP PF05183 265 - 909 7.6e-100 RNA dependent RNA polymerase
RdRP_head PF26253 961 - 1034 9.1e-12 RdRP, head domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000505)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G19910 AT2G19910 AT2G19920 AT2G19920 AT2G19920 AT2G19920 AT2G19930 AT2G19930 AT2G19930
fragaria_vesca FvH4_1g05980 FvH4_1g05990 FvH4_1g05990 FvH4_1g09718
malus_domestica MD02G1064400.v1.1 MD15G1195300.v1.1 MD15G1195400.v1.1
prunus_persica Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1
pyrus_communis pycom02g05070 pycom15g17460
rosa_chinensis RchiOBHm_Chr2g0091671 RchiOBHm_Chr2g0091681 RchiOBHm_Chr2g0091691 RchiOBHm_Chr2g0091701 RchiOBHm_Chr2g0091711 RchiOBHm_Chr2g0091721 RchiOBHm_Chr2g0091731 RchiOBHm_Chr5g0014401
rosa_laevigata RLG00000013772 RLG00000016242 RLG00000016244 RLG00000016245 RLG00000016246 RLG00000032105
rosa_multiflora Rmu_co8374041.1_g000001 Rmu_co8478121.1_g000001 Rmu_sc0000897.1_g000005 Rmu_sc0000897.1_g000009
rosa_roxburghii Rroxscaffold_1G00062180 Rroxscaffold_1G00062190 Rroxscaffold_1G00068800 Rroxscaffold_2G00149790 Rroxscaffold_2G00149800 Rroxscaffold_2G00149810
rosa_rugosa Rorug02G0019600 Rorug02G0019700 Rorug02G0019800 Rorug02G0019900 Rorug02G0019900 Rorug02G0020000 Rorug02G0020000 Rorug02G0020100 Rorug02G0020100 Rorug02G0020200 Rorug05G0017800
rosa_samantha Rh2AG064700 Rh2AG064800 Rh2AG064900 Rh2BG063800 Rh2BG063900 Rh2BG064000 Rh2BG064100 Rh2CG065500 Rh2CG065600 Rh2CG065700 Rh2DG063900 Rh2DG064000 Rh2DG064100 Rh2DG064200 Rh2DG381700 Rh5AG056700 Rh5AG056800 Rh5AG056900 Rh5AG112600 Rh5BG109300 Rh5CG063800 Rh5CG121100 Rh5DG053100 Rh5DG108300 Rh6CG100100
rosa_wichuraiana Rw2G005560 Rw2G005580 Rw2G005590 Rw5G005370 Rw5G009770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 1308, 1539, 1709
AasI GACNNNNNNGTC 1 cut(s) 86
AatII GACGTC 1 cut(s) 1147
Acc36I ACCTGC 2 cut(s) 308, 3053
AccB7I CCANNNNNTGG 2 cut(s) 681, 3016
AccIII TCCGGA 1 cut(s) 1435
AciI CCGC 8 cut(s) 23, 94, 177, 309, 979, 1493, 1513, 1564
AclI AACGTT 1 cut(s) 2830
AclWI GGATC 5 cut(s) 190, 1433, 1446, 2003, 2356
AcoI YGGCCR 3 cut(s) 74, 2814, 2862
AcsI RAATTY 3 cut(s) 560, 730, 2712
AcuI CTGAAG 2 cut(s) 281, 2421
AcvI CACGTG 1 cut(s) 1808
AcyI GRCGYC 2 cut(s) 81, 1144
AdeI CACNNNGTG 1 cut(s) 332
AfaI GTAC 7 cut(s) 55, 302, 553, 1737, 2079, 2087, 2843
AfeI AGCGCT 2 cut(s) 1815, 1948
AfiI CCNNNNNNNGG 7 cut(s) 32, 83, 90, 681, 786, 3016, 3051
AflII CTTAAG 1 cut(s) 2051
AflIII ACRYGT 1 cut(s) 1805
AgeI ACCGGT 1 cut(s) 2088
AjiI CACGTC 1 cut(s) 1041
AjnI CCWGG 3 cut(s) 114, 135, 2095
AjuI GAANNNNNNNTTGG 2 cut(s) 2377, 2409
AleI CACNNNNGTG 1 cut(s) 2138
Alw21I GWGCWC 6 cut(s) 127, 1079, 1678, 2861, 2946, 3144
Alw26I GTCTC 4 cut(s) 711, 949, 1960, 2266
Alw44I GTGCAC 1 cut(s) 123
AlwI GGATC 5 cut(s) 190, 1433, 1446, 2003, 2356
AlwNI CAGNNNCTG 1 cut(s) 847
Aor13HI TCCGGA 1 cut(s) 1435
Aor51HI AGCGCT 2 cut(s) 1815, 1948
AoxI GGCC 8 cut(s) 74, 102, 520, 805, 2130, 2814, 2862, 2954
ApaI GGGCCC 1 cut(s) 809
ApaLI GTGCAC 1 cut(s) 123
ApeKI GCWGC 7 cut(s) 346, 542, 1085, 1811, 2474, 2594, 2888
ApoI RAATTY 3 cut(s) 560, 730, 2712
AseI ATTAAT 2 cut(s) 357, 2790
AsiGI ACCGGT 1 cut(s) 2088
Asp700I GAANNNNTTC 2 cut(s) 1745, 2309
AspA2I CCTAGG 1 cut(s) 1682
AspLEI GCGC 4 cut(s) 242, 984, 1816, 1949
AspS9I GGNCC 8 cut(s) 88, 133, 339, 371, 805, 806, 1618, 2201
AsuC2I CCSGG 1 cut(s) 85
AsuHPI GGTGA 5 cut(s) 369, 491, 1038, 1850, 2258
AsuNHI GCTAGC 1 cut(s) 2488
AvaII GGWCC 6 cut(s) 88, 133, 339, 371, 1618, 2201
AvrII CCTAGG 1 cut(s) 1682
BaeGI GKGCMC 2 cut(s) 127, 809
BaeI ACNNNNGTAYC 6 cut(s) 2069, 2069, 2102, 2102, 2546, 2579
BalI TGGCCA 1 cut(s) 2816
BamHI GGATCC 1 cut(s) 1438
BanII GRGCYC 4 cut(s) 809, 1079, 2861, 3144
BarI GAAGNNNNNNTAC 3 cut(s) 32, 2337, 2369
BbrPI CACGTG 1 cut(s) 1808
BbsI GAAGAC 3 cut(s) 139, 1147, 1808
Bbv12I GWGCWC 6 cut(s) 127, 1079, 1678, 2861, 2946, 3144
BbvI GCAGC 7 cut(s) 358, 554, 1097, 1823, 2486, 2606, 2875
BcgI CGANNNNNNTGC 4 cut(s) 1514, 1548, 1564, 1598
BciT130I CCWGG 3 cut(s) 116, 137, 2097
BciVI GTATCC 2 cut(s) 429, 2103
BclI TGATCA 3 cut(s) 250, 463, 1872
BcnI CCSGG 1 cut(s) 85
BcoDI GTCTC 4 cut(s) 711, 949, 1960, 2266
BfaI CTAG 8 cut(s) 506, 909, 1683, 1721, 1892, 2489, 2588, 3090
BfmI CTRYAG 2 cut(s) 839, 1583
BfoI RGCGCY 3 cut(s) 985, 1817, 1950
BfrI CTTAAG 1 cut(s) 2051
BfuAI ACCTGC 2 cut(s) 308, 3053
BfuI GTATCC 2 cut(s) 429, 2103
BglII AGATCT 3 cut(s) 502, 1474, 2404
BlnI CCTAGG 1 cut(s) 1682
Bme1390I CCNGG 4 cut(s) 85, 116, 137, 2097
Bme18I GGWCC 6 cut(s) 88, 133, 339, 371, 1618, 2201
BmgBI CACGTC 1 cut(s) 1041
BmgT120I GGNCC 8 cut(s) 88, 133, 339, 371, 805, 806, 1618, 2201
BmiI GGNNCC 6 cut(s) 89, 807, 808, 1440, 1620, 2202
BmrFI CCNGG 4 cut(s) 85, 116, 137, 2097
BmrI ACTGGG 1 cut(s) 2266
BmsI GCATC 4 cut(s) 1072, 1771, 2158, 2581
BmtI GCTAGC 1 cut(s) 2492
BmuI ACTGGG 1 cut(s) 2266
BoxI GACNNNNGTC 2 cut(s) 233, 2065
BpiI GAAGAC 3 cut(s) 139, 1147, 1808
BplI GAGNNNNNCTC 3 cut(s) 36, 89, 121
BpmI CTGGAG 1 cut(s) 435
BpuEI CTTGAG 4 cut(s) 604, 1470, 1472, 2248
BpuMI CCSGG 1 cut(s) 85
BsaAI YACGTR 3 cut(s) 939, 1808, 2207
BsaBI GATNNNNATC 4 cut(s) 1354, 1785, 2247, 2790
BsaHI GRCGYC 2 cut(s) 81, 1144
BsaI GGTCTC 2 cut(s) 711, 2266
BsaJI CCNNGG 7 cut(s) 71, 84, 523, 1227, 1682, 2336, 2865
BsaWI WCCGGW 2 cut(s) 1435, 2088
BsaXI ACNNNNNCTCC 3 cut(s) 1660, 1690, 3131
Bsc4I CCNNNNNNNGG 7 cut(s) 32, 83, 90, 681, 786, 3016, 3051
Bse118I RCCGGY 2 cut(s) 25, 2088
Bse1I ACTGG 7 cut(s) 272, 452, 1351, 2261, 2373, 2489, 2976
Bse3DI GCAATG 5 cut(s) 444, 718, 1496, 1537, 3117
Bse8I GATNNNNATC 4 cut(s) 1354, 1785, 2247, 2790
BseAI TCCGGA 1 cut(s) 1435
BseBI CCWGG 3 cut(s) 116, 137, 2097
BseDI CCNNGG 7 cut(s) 71, 84, 523, 1227, 1682, 2336, 2865
BseGI GGATG 7 cut(s) 748, 1105, 2046, 2875, 2881, 2968, 3106
BseJI GATNNNNATC 4 cut(s) 1354, 1785, 2247, 2790
BseLI CCNNNNNNNGG 7 cut(s) 32, 83, 90, 681, 786, 3016, 3051
BseMI GCAATG 5 cut(s) 444, 718, 1496, 1537, 3117
BseMII CTCAG 8 cut(s) 48, 343, 911, 1350, 1818, 2288, 2379, 2549
BseNI ACTGG 7 cut(s) 272, 452, 1351, 2261, 2373, 2489, 2976
BseRI GAGGAG 4 cut(s) 207, 210, 213, 1668
BseSI GKGCMC 2 cut(s) 127, 809
BseXI GCAGC 7 cut(s) 358, 554, 1097, 1823, 2486, 2606, 2875
BsgI GTGCAG 2 cut(s) 1830, 1958
Bsh1285I CGRYCG 1 cut(s) 1595
BshFI GGCC 8 cut(s) 76, 104, 522, 807, 2132, 2816, 2864, 2956
BshTI ACCGGT 1 cut(s) 2088
BsiEI CGRYCG 1 cut(s) 1595
BsiHKAI GWGCWC 6 cut(s) 127, 1079, 1678, 2861, 2946, 3144
BsiSI CCGG 6 cut(s) 26, 77, 84, 91, 1436, 2089
BslFI GGGAC 6 cut(s) 907, 1020, 1443, 2014, 2214, 2783
BslI CCNNNNNNNGG 7 cut(s) 32, 83, 90, 681, 786, 3016, 3051
BsmAI GTCTC 4 cut(s) 711, 949, 1960, 2266
BsmFI GGGAC 6 cut(s) 907, 1020, 1443, 2014, 2214, 2783
BsnI GGCC 8 cut(s) 76, 104, 522, 807, 2132, 2816, 2864, 2956
Bso31I GGTCTC 2 cut(s) 711, 2266
Bsp120I GGGCCC 1 cut(s) 805
Bsp1286I GDGCHC 7 cut(s) 127, 809, 1079, 1678, 2861, 2946, 3144
Bsp13I TCCGGA 1 cut(s) 1435
Bsp19I CCATGG 3 cut(s) 71, 523, 2865
BspACI CCGC 8 cut(s) 23, 94, 177, 309, 979, 1493, 1513, 1564
BspANI GGCC 8 cut(s) 76, 104, 522, 807, 2132, 2816, 2864, 2956
BspCNI CTCAG 8 cut(s) 49, 342, 910, 1351, 1819, 2287, 2380, 2550
BspEI TCCGGA 1 cut(s) 1435
BspHI TCATGA 1 cut(s) 1132
BspLI GGNNCC 6 cut(s) 89, 807, 808, 1440, 1620, 2202
BspMAI CTGCAG 1 cut(s) 1587
BspMI ACCTGC 2 cut(s) 308, 3053
BspOI GCTAGC 1 cut(s) 2492
BspPI GGATC 5 cut(s) 190, 1433, 1446, 2003, 2356
BspTI CTTAAG 1 cut(s) 2051
BspTNI GGTCTC 2 cut(s) 711, 2266
BsrDI GCAATG 5 cut(s) 444, 718, 1496, 1537, 3117
BsrFI RCCGGY 2 cut(s) 25, 2088
BsrI ACTGG 7 cut(s) 272, 452, 1351, 2261, 2373, 2489, 2976
BssAI RCCGGY 2 cut(s) 25, 2088
BssECI CCNNGG 7 cut(s) 71, 84, 523, 1227, 1682, 2336, 2865
BssNI GRCGYC 2 cut(s) 81, 1144
BssT1I CCWWGG 6 cut(s) 71, 523, 1227, 1682, 2336, 2865
Bst2UI CCWGG 3 cut(s) 116, 137, 2097
Bst6I CTCTTC 2 cut(s) 695, 1332
BstACI GRCGYC 2 cut(s) 81, 1144
BstAFI CTTAAG 1 cut(s) 2051
BstBAI YACGTR 3 cut(s) 939, 1808, 2207
BstC8I GCNNGC 5 cut(s) 102, 435, 977, 2490, 3080
BstDSI CCRYGG 3 cut(s) 71, 523, 2865
BstF5I GGATG 7 cut(s) 748, 1105, 2046, 2875, 2881, 2968, 3106
BstH2I RGCGCY 3 cut(s) 985, 1817, 1950
BstHHI GCGC 4 cut(s) 242, 984, 1816, 1949
BstMAI GTCTC 4 cut(s) 711, 949, 1960, 2266
BstMCI CGRYCG 1 cut(s) 1595
BstMWI GCNNNNNNNGC 8 cut(s) 73, 239, 323, 539, 981, 2177, 2591, 3068
BstNI CCWGG 3 cut(s) 116, 137, 2097
BstNSI RCATGY 1 cut(s) 1160
BstPAI GACNNNNGTC 2 cut(s) 233, 2065
BstSCI CCNGG 4 cut(s) 83, 114, 135, 2095
BstSFI CTRYAG 2 cut(s) 839, 1583
BstSLI GKGCMC 2 cut(s) 127, 809
BstSNI TACGTA 1 cut(s) 939
BstV1I GCAGC 7 cut(s) 358, 554, 1097, 1823, 2486, 2606, 2875
BstV2I GAAGAC 3 cut(s) 139, 1147, 1808
BstX2I RGATCY 4 cut(s) 502, 1438, 1474, 2404
BstYI RGATCY 4 cut(s) 502, 1438, 1474, 2404
BsuI GTATCC 2 cut(s) 429, 2103
BsuRI GGCC 8 cut(s) 76, 104, 522, 807, 2132, 2816, 2864, 2956
BtgI CCRYGG 3 cut(s) 71, 523, 2865
BtgZI GCGATG 2 cut(s) 1299, 2566
BtrI CACGTC 1 cut(s) 1041
BtsCI GGATG 7 cut(s) 748, 1105, 2046, 2875, 2881, 2968, 3106
BtsI GCAGTG 1 cut(s) 1580
BtsIMutI CAGTG 6 cut(s) 337, 459, 1514, 1580, 2157, 2397
BveI ACCTGC 2 cut(s) 308, 3053
Cac8I GCNNGC 5 cut(s) 102, 435, 977, 2490, 3080
CaiI CAGNNNCTG 1 cut(s) 847
CciI TCATGA 1 cut(s) 1132
CfoI GCGC 4 cut(s) 242, 984, 1816, 1949
Cfr10I RCCGGY 2 cut(s) 25, 2088
Cfr13I GGNCC 8 cut(s) 88, 133, 339, 371, 805, 806, 1618, 2201
CpoI CGGWCCG 1 cut(s) 339
CseI GACGC 3 cut(s) 89, 759, 2032
Csp6I GTAC 7 cut(s) 54, 301, 552, 1736, 2078, 2086, 2842
CspAI ACCGGT 1 cut(s) 2088
CspI CGGWCCG 1 cut(s) 339
CviQI GTAC 7 cut(s) 54, 301, 552, 1736, 2078, 2086, 2842
DraI TTTAAA 1 cut(s) 1003
DraIII CACNNNGTG 1 cut(s) 332
DrdI GACNNNNNNGTC 1 cut(s) 86
DseDI GACNNNNNNGTC 1 cut(s) 86
EaeI YGGCCR 3 cut(s) 74, 2814, 2862
Eam1104I CTCTTC 2 cut(s) 695, 1332
EarI CTCTTC 2 cut(s) 695, 1332
EciI GGCGGA 1 cut(s) 12
Ecl136II GAGCTC 3 cut(s) 1077, 2859, 3142
Eco105I TACGTA 1 cut(s) 939
Eco130I CCWWGG 6 cut(s) 71, 523, 1227, 1682, 2336, 2865
Eco24I GRGCYC 4 cut(s) 809, 1079, 2861, 3144
Eco31I GGTCTC 2 cut(s) 711, 2266
Eco32I GATATC 1 cut(s) 163
Eco47I GGWCC 6 cut(s) 88, 133, 339, 371, 1618, 2201
Eco47III AGCGCT 2 cut(s) 1815, 1948
Eco53kI GAGCTC 3 cut(s) 1077, 2859, 3142
Eco57I CTGAAG 2 cut(s) 281, 2421
Eco72I CACGTG 1 cut(s) 1808
EcoICRI GAGCTC 3 cut(s) 1077, 2859, 3142
EcoO109I RGGNCCY 4 cut(s) 133, 805, 806, 1618
EcoRI GAATTC 1 cut(s) 560
EcoRII CCWGG 3 cut(s) 114, 135, 2095
EcoRV GATATC 1 cut(s) 163
EcoT14I CCWWGG 6 cut(s) 71, 523, 1227, 1682, 2336, 2865
EcoT22I ATGCAT 1 cut(s) 1158
EcoT38I GRGCYC 4 cut(s) 809, 1079, 2861, 3144
ErhI CCWWGG 6 cut(s) 71, 523, 1227, 1682, 2336, 2865
FalI AAGNNNNNCTT 5 cut(s) 1953, 1985, 2320, 2352, 3128
FaqI GGGAC 6 cut(s) 907, 1020, 1443, 2014, 2214, 2783
FauI CCCGC 1 cut(s) 87
FbaI TGATCA 3 cut(s) 250, 463, 1872
FokI GGATG 7 cut(s) 755, 1112, 2033, 2882, 2888, 2975, 3113
FriOI GRGCYC 4 cut(s) 809, 1079, 2861, 3144
FspBI CTAG 8 cut(s) 506, 909, 1683, 1721, 1892, 2489, 2588, 3090
GlaI GCGC 4 cut(s) 241, 983, 1815, 1948
GsuI CTGGAG 1 cut(s) 435
HaeII RGCGCY 3 cut(s) 985, 1817, 1950
HaeIII GGCC 8 cut(s) 76, 104, 522, 807, 2132, 2816, 2864, 2956
HapII CCGG 6 cut(s) 26, 77, 84, 91, 1436, 2089
HgaI GACGC 3 cut(s) 89, 759, 2032
HhaI GCGC 4 cut(s) 242, 984, 1816, 1949
Hin1I GRCGYC 2 cut(s) 81, 1144
Hin6I GCGC 4 cut(s) 240, 982, 1814, 1947
HinP1I GCGC 4 cut(s) 240, 982, 1814, 1947
HincII GTYRAC 1 cut(s) 1276
HindII GTYRAC 1 cut(s) 1276
HindIII AAGCTT 1 cut(s) 1689
HpaII CCGG 6 cut(s) 26, 77, 84, 91, 1436, 2089
HphI GGTGA 5 cut(s) 369, 491, 1038, 1850, 2258
Hpy166II GTNNAC 7 cut(s) 125, 1180, 1276, 1736, 2086, 2102, 2333
Hpy8I GTNNAC 7 cut(s) 125, 1180, 1276, 1736, 2086, 2102, 2333
HpyCH4IV ACGT 8 cut(s) 121, 938, 1040, 1144, 1807, 2206, 2541, 2830
HpyF10VI GCNNNNNNNGC 8 cut(s) 73, 239, 323, 539, 981, 2177, 2591, 3068
HpySE526I ACGT 8 cut(s) 121, 938, 1040, 1144, 1807, 2206, 2541, 2830
Hsp92I GRCGYC 2 cut(s) 81, 1144
HspAI GCGC 4 cut(s) 240, 982, 1814, 1947
Kpn2I TCCGGA 1 cut(s) 1435
Ksp22I TGATCA 3 cut(s) 250, 463, 1872
LmnI GCTCC 7 cut(s) 45, 97, 311, 454, 1681, 1862, 3139
Lsp1109I GCAGC 7 cut(s) 358, 554, 1097, 1823, 2486, 2606, 2875
LweI GCATC 4 cut(s) 1072, 1771, 2158, 2581
MaeI CTAG 8 cut(s) 506, 909, 1683, 1721, 1892, 2489, 2588, 3090
MaeII ACGT 8 cut(s) 121, 938, 1040, 1144, 1807, 2206, 2541, 2830
MflI RGATCY 4 cut(s) 502, 1438, 1474, 2404
MhlI GDGCHC 7 cut(s) 127, 809, 1079, 1678, 2861, 2946, 3144
MlsI TGGCCA 1 cut(s) 2816
MluNI TGGCCA 1 cut(s) 2816
MlyI GAGTC 3 cut(s) 680, 889, 1976
MmeI TCCRAC 3 cut(s) 251, 446, 2036
Mox20I TGGCCA 1 cut(s) 2816
Mph1103I ATGCAT 1 cut(s) 1158
MroI TCCGGA 1 cut(s) 1435
MroXI GAANNNNTTC 2 cut(s) 1745, 2309
MscI TGGCCA 1 cut(s) 2816
MslI CAYNNNNRTG 4 cut(s) 762, 1338, 1731, 2138
Msp20I TGGCCA 1 cut(s) 2816
MspA1I CMGCKG 2 cut(s) 233, 2006
MspCI CTTAAG 1 cut(s) 2051
MspI CCGG 6 cut(s) 26, 77, 84, 91, 1436, 2089
MspR9I CCNGG 4 cut(s) 85, 116, 137, 2097
MvaI CCWGG 3 cut(s) 116, 137, 2097
MwoI GCNNNNNNNGC 8 cut(s) 73, 239, 323, 539, 981, 2177, 2591, 3068
NciI CCSGG 1 cut(s) 85
NcoI CCATGG 3 cut(s) 71, 523, 2865
NheI GCTAGC 1 cut(s) 2488
NlaIV GGNNCC 6 cut(s) 89, 807, 808, 1440, 1620, 2202
NmeAIII GCCGAG 2 cut(s) 153, 2840
NmuCI GTSAC 3 cut(s) 1242, 1920, 2640
NsiI ATGCAT 1 cut(s) 1158
NspI RCATGY 1 cut(s) 1160
OliI CACNNNNGTG 1 cut(s) 2138
PagI TCATGA 1 cut(s) 1132
PdmI GAANNNNTTC 2 cut(s) 1745, 2309
PfeI GAWTC 7 cut(s) 48, 1289, 1350, 1588, 1898, 2020, 2153
PflMI CCANNNNNTGG 2 cut(s) 681, 3016
PinAI ACCGGT 1 cut(s) 2088
PleI GAGTC 3 cut(s) 679, 889, 1976
PmaCI CACGTG 1 cut(s) 1808
PmlI CACGTG 1 cut(s) 1808
PpsI GAGTC 3 cut(s) 679, 889, 1976
Ppu21I YACGTR 3 cut(s) 939, 1808, 2207
PpuMI RGGWCCY 2 cut(s) 133, 1618
PshAI GACNNNNGTC 2 cut(s) 233, 2065
PshBI ATTAAT 2 cut(s) 357, 2790
PsiI TTATAA 3 cut(s) 1308, 1539, 1709
Psp124BI GAGCTC 3 cut(s) 1079, 2861, 3144
Psp1406I AACGTT 1 cut(s) 2830
Psp5II RGGWCCY 2 cut(s) 133, 1618
Psp6I CCWGG 3 cut(s) 114, 135, 2095
PspCI CACGTG 1 cut(s) 1808
PspGI CCWGG 3 cut(s) 114, 135, 2095
PspN4I GGNNCC 6 cut(s) 89, 807, 808, 1440, 1620, 2202
PspOMI GGGCCC 1 cut(s) 805
PspPI GGNCC 8 cut(s) 88, 133, 339, 371, 805, 806, 1618, 2201
PspPPI RGGWCCY 2 cut(s) 133, 1618
PstI CTGCAG 1 cut(s) 1587
PstNI CAGNNNCTG 1 cut(s) 847
PsuI RGATCY 4 cut(s) 502, 1438, 1474, 2404
PvuII CAGCTG 2 cut(s) 233, 2006
RsaI GTAC 7 cut(s) 55, 302, 553, 1737, 2079, 2087, 2843
RsaNI GTAC 7 cut(s) 54, 301, 552, 1736, 2078, 2086, 2842
RseI CAYNNNNRTG 4 cut(s) 762, 1338, 1731, 2138
Rsr2I CGGWCCG 1 cut(s) 339
RsrII CGGWCCG 1 cut(s) 339
SacI GAGCTC 3 cut(s) 1079, 2861, 3144
Sau96I GGNCC 8 cut(s) 88, 133, 339, 371, 805, 806, 1618, 2201
SchI GAGTC 3 cut(s) 680, 889, 1976
ScrFI CCNGG 4 cut(s) 85, 116, 137, 2097
SduI GDGCHC 7 cut(s) 127, 809, 1079, 1678, 2861, 2946, 3144
SfaNI GCATC 4 cut(s) 1072, 1771, 2158, 2581
SfcI CTRYAG 2 cut(s) 839, 1583
SgrAI CRCCGGYG 1 cut(s) 25
SinI GGWCC 6 cut(s) 88, 133, 339, 371, 1618, 2201
SmiMI CAYNNNNRTG 4 cut(s) 762, 1338, 1731, 2138
SmlI CTYRAG 5 cut(s) 583, 1451, 1485, 2051, 2263
SmoI CTYRAG 5 cut(s) 583, 1451, 1485, 2051, 2263
SnaBI TACGTA 1 cut(s) 939
SsiI CCGC 8 cut(s) 23, 94, 177, 309, 979, 1493, 1513, 1564
SspMI CTAG 8 cut(s) 506, 909, 1683, 1721, 1892, 2489, 2588, 3090
SstI GAGCTC 3 cut(s) 1079, 2861, 3144
StyD4I CCNGG 4 cut(s) 83, 114, 135, 2095
StyI CCWWGG 6 cut(s) 71, 523, 1227, 1682, 2336, 2865
TaiI ACGT 8 cut(s) 124, 941, 1043, 1147, 1810, 2209, 2544, 2833
TaqI TCGA 3 cut(s) 735, 1591, 2699
TaqII GACCGA 2 cut(s) 356, 735
TatI WGTACW 2 cut(s) 53, 300
TauI GCSGC 3 cut(s) 982, 1515, 1567
TfiI GAWTC 7 cut(s) 48, 1289, 1350, 1588, 1898, 2020, 2153
TscAI CASTG 6 cut(s) 337, 459, 1514, 1587, 2164, 2404
TseFI GTSAC 3 cut(s) 1242, 1920, 2640
TseI GCWGC 7 cut(s) 346, 542, 1085, 1811, 2474, 2594, 2888
Tsp45I GTSAC 3 cut(s) 1242, 1920, 2640
TspGWI ACGGA 3 cut(s) 32, 188, 2073
TspRI CASTG 6 cut(s) 337, 459, 1514, 1587, 2164, 2404
Van91I CCANNNNNTGG 2 cut(s) 681, 3016
Vha464I CTTAAG 1 cut(s) 2051
VneI GTGCAC 1 cut(s) 123
VpaK11BI GGWCC 6 cut(s) 88, 133, 339, 371, 1618, 2201
VspI ATTAAT 2 cut(s) 357, 2790
XapI RAATTY 3 cut(s) 560, 730, 2712
XbaI TCTAGA 3 cut(s) 505, 1891, 2587
XceI RCATGY 1 cut(s) 1160
XcmI CCANNNNNNNNNTGG 2 cut(s) 1651, 2334
XmaJI CCTAGG 1 cut(s) 1682
XmnI GAANNNNTTC 2 cut(s) 1745, 2309
XspI CTAG 8 cut(s) 506, 909, 1683, 1721, 1892, 2489, 2588, 3090
ZraI GACGTC 1 cut(s) 1145
Zsp2I ATGCAT 1 cut(s) 1158
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.