Rh2CG065500
ERF Family

Probably involved in the RNA silencing pathway and required for the generation of small interfering RNAs (siRNAs)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0000505
Physical Location & Seq
Forward (+)
5433686 .. 5445244
11559 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG065500.1

Sequence Viewer

Length: 2799 bp
ATGGAAGAAGTGAGATTACCTCCGCCGGTGGAGGAGTTGCTCCGTAGAATCTGTACTGAGAAAAAGTTACCATGGCCGGACGCCGGGGTCCGGCGGGAGCTGGTCTCTATCAGCCAGGAACGTGCACATGAGGTCCTGGTTAAAGTCTTCAACAAACAAGATATCATAAGTCTCGGCGGTTTCATCAAGCACTTGATCCGTAACCTCCCTGCTTCTCCTCCTCCTTCGACAGCTGTCCGCGCTTATTATGATCAAAGACCCTCCGCTTGTCCAGTTACGCCACCTTGTTCTCAACTTCAGTACAGCTCCGCAGTTGCAGGTCAGGCATTCAGTGATCGGACCGAGCAGCCTGTGATTAATGGACGGTATGGACCATCACCATATTCTTCTGGTTGTAAAGCCATCAGCTCTTTCTCGTATCCAACAATACCGCTGGCTTGTAACATTGCTCCAGTGCAACTTGATCATAGGTTTCAAGGTGAGGTTGTAACCAAGTCGCCAGATCTAGAGAAATATGGAGGCCATGGACAAGCACACTTAATTGGCAGCTCTTGGGAAAAGTTAAAGAATGTTATAGTCGCAGAGGATATTAAGCATTTGAAAGATTTGCCAATGGCAGAGTTTGAGTCCAAAAAAAGATCCCTGGGCCCCTATCTACAAAATGAAAGAAGAACTCTCCTACAGAAACTATGTCAAGATGACAATGTTTTGATTGTGAAGTTTGATGAAGAAGGGACTCAGACAGGTTCTAGGACTTCTTATGATAATGACTATTCTACGTATATGAAGGTTGAGAGACAAGGGATTTTTGTTGGCTTGCGGCGCTATCGTTTCTTTGGTTTTAAAGATGGTGGTAAAAGAGATAAAATCAGAAGTCCCACGTCATCACCCGTAAAATGTTATTTTGTTCGTGTGAGCTCTGATGCAGCAATAGACAGGGATGTAGGTTATTGGTTATCTAACAGAACAATTCATGAAGGAAGACGTCTAATCATGCATGTTGATACTCTGCCAAGTGTGGACAAGGTCATGGCTAGGTTGCCACTCATTTTTTCTAAGACAACGACCTTGGAAGTAGATTGGTCACTTGTGAAAGTTGAAGAAATTGTTGACGTCAACTGTTGCGATGAATCTGGTAACTGTGTTTATAATGAAGATGGAAAACCTCTTCTACATACAGATGGAACTGGATTCATATCTGAGGATTTGGCTTTGCTTTGTCCAAAGATAGAAGCCAAAAGAAAGTTTATAAGTGAGGAACACATGGAGGGACTTCCGGATCCTGATGAACTTGAGATTGAAATGAAAAAATCAGATCTCCGAACTCAAGAACCGCCATTGCTCATACAGTGCCGCCTATTTCACGAAGGCAATGCTTATAAAGGAACTTTGCTTGTCAATAAGCGGCTTCCCCCGAAAACACTGCAGATTCGACCGTCAATGCGTAAAGTTGAGAAGGACCCTAAGATTTCAGATAATGAAACCATCAATTCTTTGGAAATAGTTTGCACAAGTGCTCCTCCTAGGAAAGCTTATTTGTCAAGGATTATAATTGCACTTCTAGTGCATGGAGGTGTACCGAAAGACTTCTTTATGAAGATACTTGACAATGCTTTAGAAGATGCTCATCGTGTTATCGGGAAGACACGTGCTGCGCTCAAAGTTGCTGAGAAGTATGCTGATCCAACAGGGATTCTGGAAAGAGATGAAGTCTGCGTTATCCTGAAGGACGGACAAATGTCGGGGAAGGTACTTGTGTACCGGTATCCTGGTTTACACTTCGGGGATATTAAAGTTTTGAAGGCCACCTATGTGAAGGAGTTAGAATCCATTGTGGGAGATGCCAGATATGCTATATTCTTCTCTTGCAAGGGACCACGTAATGTTGCTGATGAAATTGGTGGTGGAGATTTTGATGGTGATCTGTACTGGGTCTCAAGAAACCCTCAGCTATTGGAATGGTACAAACCAAGTGAACCTTGGAACGAAGGATCAATATCAACTCAAAAGGTTGCCAGTAATAGACCAACTGAGCTTTCCACTGAAGATCTTGAAGCTGATATGTTTAGATTGTTCTTGAAAAATAGGTTTCAACCAAGTTTTGCTATGAGTGAGGCAGCTAATAACTGGCTAGCTTGGATAGATTGGTTCTTGACTTTGGGAGATAGTAATGAGAAGAAGGACGTAATGGACAAGATACTGAGGTTGGTTGATATATACTACATCGCTCTAGATGCAGCGAAGAAAGGTGTAAAGGTTGAAGTTCCAAAAGATTTGAAGGTGTCACTTTTCCCTCATTACATGGACAAGAAAAAAGAAGAAGCAACTTACATTTCATCTTCGATTTTGGGAGAAATTTATGATGAAGTTGATAAATGTCAAGCAAAAATAATTCTTCTCAGAGAAATCAAGAAACTTCCTATTTTTGATATTAATCAAGTCCCCGAACTCTGCTTGGCCAAATGGAAGAAACGTTATGGACGGTACAGGACTGAAATGAGCTCGGCCATGGATGAGGATGGTGATGGAGATCAGGCTGCCATTGTCATAAGAAAGTATAAAAAGAAGCTGTATAAGGCTGATGACTTTGAGCACAGTTCAAGGCCGATGGATGAAATACTGGACGAGGCACACGCTATATATCATGTATGTTATGAGCATGCTATGGATAAAAGAGATGTGAAAAAATGTTCCTTTGCATGGAGAGTTGCAGGTAAAGCTCTGTGCGAGCTTCACGCTAGGAAAAAGGATGAGAAGTGGTTAAACATTGCCCCTTCTGTTTTGAAAGGGCTCTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RdRP

Protein Analysis

932

Amino Acids

105.94

Weight (kDa)

7.9

Isoelectric Point (pI)

43.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
4HB_RdRP3_N PF26249 4 - 67 1.7e-06 RdRP3/4/5, N-terminal four helical bundle domain
RdRP PF05183 216 - 558 2.5e-44 RNA dependent RNA polymerase
RdRP PF05183 560 - 790 1.3e-44 RNA dependent RNA polymerase
RdRP_head PF26253 844 - 917 1.2e-11 RdRP, head domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000505)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G19910 AT2G19910 AT2G19920 AT2G19920 AT2G19920 AT2G19920 AT2G19930 AT2G19930 AT2G19930
fragaria_vesca FvH4_1g05980 FvH4_1g05990 FvH4_1g05990 FvH4_1g09718
malus_domestica MD02G1064400.v1.1 MD15G1195300.v1.1 MD15G1195400.v1.1
prunus_persica Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1
pyrus_communis pycom02g05070 pycom15g17460
rosa_chinensis RchiOBHm_Chr2g0091671 RchiOBHm_Chr2g0091681 RchiOBHm_Chr2g0091691 RchiOBHm_Chr2g0091701 RchiOBHm_Chr2g0091711 RchiOBHm_Chr2g0091721 RchiOBHm_Chr2g0091731 RchiOBHm_Chr5g0014401
rosa_laevigata RLG00000013772 RLG00000016242 RLG00000016244 RLG00000016245 RLG00000016246 RLG00000032105
rosa_multiflora Rmu_co8374041.1_g000001 Rmu_co8478121.1_g000001 Rmu_sc0000897.1_g000005 Rmu_sc0000897.1_g000009
rosa_roxburghii Rroxscaffold_1G00062180 Rroxscaffold_1G00062190 Rroxscaffold_1G00068800 Rroxscaffold_2G00149790 Rroxscaffold_2G00149800 Rroxscaffold_2G00149810
rosa_rugosa Rorug02G0019600 Rorug02G0019700 Rorug02G0019800 Rorug02G0019900 Rorug02G0019900 Rorug02G0020000 Rorug02G0020000 Rorug02G0020100 Rorug02G0020100 Rorug02G0020200 Rorug05G0017800
rosa_samantha Rh2AG064700 Rh2AG064800 Rh2AG064900 Rh2BG063800 Rh2BG063900 Rh2BG064000 Rh2BG064100 Rh2CG065500 Rh2CG065600 Rh2CG065700 Rh2DG063900 Rh2DG064000 Rh2DG064100 Rh2DG064200 Rh2DG381700 Rh5AG056700 Rh5AG056800 Rh5AG056900 Rh5AG112600 Rh5BG109300 Rh5CG063800 Rh5CG121100 Rh5DG053100 Rh5DG108300 Rh6CG100100
rosa_wichuraiana Rw2G005560 Rw2G005580 Rw2G005590 Rw5G005370 Rw5G009770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 4 cut(s) 1149, 1250, 1380, 1550
AasI GACNNNNNNGTC 1 cut(s) 86
AatII GACGTC 2 cut(s) 988, 1116
Acc36I ACCTGC 2 cut(s) 308, 2702
AccII CGCG 1 cut(s) 240
AccIII TCCGGA 1 cut(s) 1276
AclI AACGTT 1 cut(s) 2473
AclWI GGATC 6 cut(s) 190, 633, 1274, 1287, 1676, 1999
AcoI YGGCCR 3 cut(s) 74, 2457, 2505
AcsI RAATTY 1 cut(s) 2355
AcuI CTGAAG 3 cut(s) 281, 1745, 2064
AcvI CACGTG 1 cut(s) 1649
AcyI GRCGYC 3 cut(s) 81, 985, 1113
AfaI GTAC 8 cut(s) 55, 302, 1578, 1752, 1760, 1928, 1964, 2486
AfiI CCNNNNNNNGG 3 cut(s) 83, 90, 2700
AflIII ACRYGT 1 cut(s) 1646
AgeI ACCGGT 1 cut(s) 1761
AjiI CACGTC 1 cut(s) 882
AjnI CCWGG 4 cut(s) 114, 135, 642, 1768
AjuI GAANNNNNNNTTGG 2 cut(s) 2020, 2052
AleI CACNNNNGTG 1 cut(s) 1811
Alw21I GWGCWC 5 cut(s) 127, 920, 1519, 2504, 2595
Alw26I GTCTC 4 cut(s) 109, 176, 790, 1939
Alw44I GTGCAC 1 cut(s) 123
AlwI GGATC 6 cut(s) 190, 633, 1274, 1287, 1676, 1999
Aor13HI TCCGGA 1 cut(s) 1276
AoxI GGCC 7 cut(s) 74, 520, 646, 1803, 2457, 2505, 2603
ApaI GGGCCC 1 cut(s) 650
ApaLI GTGCAC 1 cut(s) 123
ApeKI GCWGC 7 cut(s) 346, 546, 926, 1652, 2117, 2237, 2537
ApoI RAATTY 1 cut(s) 2355
AseI ATTAAT 2 cut(s) 357, 2433
AsiGI ACCGGT 1 cut(s) 1761
Asp700I GAANNNNTTC 1 cut(s) 1586
AspA2I CCTAGG 1 cut(s) 1523
AspLEI GCGC 3 cut(s) 242, 825, 1657
AspS9I GGNCC 8 cut(s) 88, 133, 339, 371, 646, 647, 1459, 1874
AsuC2I CCSGG 1 cut(s) 85
AsuHPI GGTGA 5 cut(s) 369, 491, 879, 1931, 2534
AsuNHI GCTAGC 1 cut(s) 2131
AvaII GGWCC 6 cut(s) 88, 133, 339, 371, 1459, 1874
AvrII CCTAGG 1 cut(s) 1523
BaeGI GKGCMC 2 cut(s) 127, 650
BaeI ACNNNNGTAYC 6 cut(s) 1742, 1742, 1775, 1775, 2189, 2222
BalI TGGCCA 1 cut(s) 2459
BamHI GGATCC 1 cut(s) 1279
BanII GRGCYC 4 cut(s) 650, 920, 2504, 2793
BarI GAAGNNNNNNTAC 1 cut(s) 32
BbrPI CACGTG 1 cut(s) 1649
BbsI GAAGAC 3 cut(s) 139, 988, 1649
Bbv12I GWGCWC 5 cut(s) 127, 920, 1519, 2504, 2595
BbvCI CCTCAGC 1 cut(s) 1947
BbvI GCAGC 7 cut(s) 358, 558, 938, 1639, 2129, 2249, 2524
BcgI CGANNNNNNTGC 4 cut(s) 1355, 1389, 1405, 1439
BciT130I CCWGG 4 cut(s) 116, 137, 644, 1770
BciVI GTATCC 2 cut(s) 429, 1776
BclI TGATCA 2 cut(s) 250, 463
BcnI CCSGG 1 cut(s) 85
BcoDI GTCTC 4 cut(s) 109, 176, 790, 1939
BfaI CTAG 8 cut(s) 506, 750, 1035, 1524, 1562, 2132, 2231, 2739
BfmI CTRYAG 2 cut(s) 680, 1424
BfoI RGCGCY 1 cut(s) 826
BfuAI ACCTGC 2 cut(s) 308, 2702
BfuI GTATCC 2 cut(s) 429, 1776
BglII AGATCT 3 cut(s) 502, 1315, 2047
BlnI CCTAGG 1 cut(s) 1523
Bme1390I CCNGG 5 cut(s) 85, 116, 137, 644, 1770
Bme18I GGWCC 6 cut(s) 88, 133, 339, 371, 1459, 1874
BmgBI CACGTC 1 cut(s) 882
BmgT120I GGNCC 8 cut(s) 88, 133, 339, 371, 646, 647, 1459, 1874
BmiI GGNNCC 6 cut(s) 89, 648, 649, 1281, 1461, 1875
BmrFI CCNGG 5 cut(s) 85, 116, 137, 644, 1770
BmrI ACTGGG 1 cut(s) 1939
BmsI GCATC 4 cut(s) 913, 1612, 1831, 2224
BmtI GCTAGC 1 cut(s) 2135
BmuI ACTGGG 1 cut(s) 1939
BoxI GACNNNNGTC 2 cut(s) 233, 1738
BpiI GAAGAC 3 cut(s) 139, 988, 1649
BplI GAGNNNNNCTC 3 cut(s) 36, 89, 121
BpmI CTGGAG 1 cut(s) 435
Bpu10I CCTNAGC 1 cut(s) 1947
BpuEI CTTGAG 3 cut(s) 1311, 1313, 1921
BpuMI CCSGG 1 cut(s) 85
BsaAI YACGTR 3 cut(s) 780, 1649, 1880
BsaBI GATNNNNATC 6 cut(s) 1195, 1626, 1920, 1996, 2433, 2529
BsaHI GRCGYC 3 cut(s) 81, 985, 1113
BsaI GGTCTC 2 cut(s) 109, 1939
BsaJI CCNNGG 9 cut(s) 71, 84, 523, 642, 643, 1068, 1523, 1979, 2508
BsaWI WCCGGW 2 cut(s) 1276, 1761
BsaXI ACNNNNNCTCC 2 cut(s) 1501, 1531
Bsc4I CCNNNNNNNGG 3 cut(s) 83, 90, 2700
Bse118I RCCGGY 2 cut(s) 25, 1761
Bse1I ACTGG 7 cut(s) 272, 452, 1192, 1934, 2016, 2132, 2625
Bse3DI GCAATG 4 cut(s) 444, 1337, 1378, 2766
Bse8I GATNNNNATC 6 cut(s) 1195, 1626, 1920, 1996, 2433, 2529
BseAI TCCGGA 1 cut(s) 1276
BseBI CCWGG 4 cut(s) 116, 137, 644, 1770
BseDI CCNNGG 9 cut(s) 71, 84, 523, 642, 643, 1068, 1523, 1979, 2508
BseGI GGATG 5 cut(s) 946, 2518, 2524, 2617, 2755
BseJI GATNNNNATC 6 cut(s) 1195, 1626, 1920, 1996, 2433, 2529
BseLI CCNNNNNNNGG 3 cut(s) 83, 90, 2700
BseMI GCAATG 4 cut(s) 444, 1337, 1378, 2766
BseMII CTCAG 8 cut(s) 48, 752, 1191, 1659, 1961, 2022, 2192, 2413
BseNI ACTGG 7 cut(s) 272, 452, 1192, 1934, 2016, 2132, 2625
BseRI GAGGAG 4 cut(s) 47, 207, 210, 1509
BseSI GKGCMC 2 cut(s) 127, 650
BseXI GCAGC 7 cut(s) 358, 558, 938, 1639, 2129, 2249, 2524
Bsh1236I CGCG 1 cut(s) 240
Bsh1285I CGRYCG 1 cut(s) 1436
BshFI GGCC 7 cut(s) 76, 522, 648, 1805, 2459, 2507, 2605
BshTI ACCGGT 1 cut(s) 1761
BsiEI CGRYCG 1 cut(s) 1436
BsiHKAI GWGCWC 5 cut(s) 127, 920, 1519, 2504, 2595
BsiSI CCGG 6 cut(s) 26, 77, 84, 91, 1277, 1762
BslFI GGGAC 5 cut(s) 748, 861, 1284, 1887, 2426
BslI CCNNNNNNNGG 3 cut(s) 83, 90, 2700
BsmAI GTCTC 4 cut(s) 109, 176, 790, 1939
BsmFI GGGAC 5 cut(s) 748, 861, 1284, 1887, 2426
BsmI GAATGC 1 cut(s) 326
BsnI GGCC 7 cut(s) 76, 522, 648, 1805, 2459, 2507, 2605
Bso31I GGTCTC 2 cut(s) 109, 1939
Bsp120I GGGCCC 1 cut(s) 646
Bsp1286I GDGCHC 7 cut(s) 127, 650, 920, 1519, 2504, 2595, 2793
Bsp13I TCCGGA 1 cut(s) 1276
Bsp19I CCATGG 3 cut(s) 71, 523, 2508
BspANI GGCC 7 cut(s) 76, 522, 648, 1805, 2459, 2507, 2605
BspCNI CTCAG 8 cut(s) 49, 751, 1192, 1660, 1960, 2023, 2193, 2412
BspEI TCCGGA 1 cut(s) 1276
BspFNI CGCG 1 cut(s) 240
BspHI TCATGA 1 cut(s) 973
BspLI GGNNCC 6 cut(s) 89, 648, 649, 1281, 1461, 1875
BspMAI CTGCAG 1 cut(s) 1428
BspMI ACCTGC 2 cut(s) 308, 2702
BspOI GCTAGC 1 cut(s) 2135
BspPI GGATC 6 cut(s) 190, 633, 1274, 1287, 1676, 1999
BspTNI GGTCTC 2 cut(s) 109, 1939
BsrDI GCAATG 4 cut(s) 444, 1337, 1378, 2766
BsrFI RCCGGY 2 cut(s) 25, 1761
BsrI ACTGG 7 cut(s) 272, 452, 1192, 1934, 2016, 2132, 2625
BssAI RCCGGY 2 cut(s) 25, 1761
BssECI CCNNGG 9 cut(s) 71, 84, 523, 642, 643, 1068, 1523, 1979, 2508
BssNI GRCGYC 3 cut(s) 81, 985, 1113
BssT1I CCWWGG 6 cut(s) 71, 523, 1068, 1523, 1979, 2508
Bst2UI CCWGG 4 cut(s) 116, 137, 644, 1770
Bst4CI ACNGT 7 cut(s) 366, 1121, 1142, 1350, 1437, 2484, 2597
Bst6I CTCTTC 1 cut(s) 1173
BstACI GRCGYC 3 cut(s) 81, 985, 1113
BstBAI YACGTR 3 cut(s) 780, 1649, 1880
BstC8I GCNNGC 5 cut(s) 435, 818, 2133, 2661, 2729
BstDSI CCRYGG 3 cut(s) 71, 523, 2508
BstF5I GGATG 5 cut(s) 946, 2518, 2524, 2617, 2755
BstFNI CGCG 1 cut(s) 240
BstH2I RGCGCY 1 cut(s) 826
BstHHI GCGC 3 cut(s) 242, 825, 1657
BstMAI GTCTC 4 cut(s) 109, 176, 790, 1939
BstMCI CGRYCG 1 cut(s) 1436
BstMWI GCNNNNNNNGC 6 cut(s) 239, 323, 822, 1850, 2234, 2717
BstNI CCWGG 4 cut(s) 116, 137, 644, 1770
BstNSI RCATGY 2 cut(s) 1001, 2663
BstPAI GACNNNNGTC 2 cut(s) 233, 1738
BstSCI CCNGG 5 cut(s) 83, 114, 135, 642, 1768
BstSFI CTRYAG 2 cut(s) 680, 1424
BstSLI GKGCMC 2 cut(s) 127, 650
BstSNI TACGTA 1 cut(s) 780
BstUI CGCG 1 cut(s) 240
BstV1I GCAGC 7 cut(s) 358, 558, 938, 1639, 2129, 2249, 2524
BstV2I GAAGAC 3 cut(s) 139, 988, 1649
BstX2I RGATCY 5 cut(s) 502, 638, 1279, 1315, 2047
BstYI RGATCY 5 cut(s) 502, 638, 1279, 1315, 2047
BsuI GTATCC 2 cut(s) 429, 1776
BsuRI GGCC 7 cut(s) 76, 522, 648, 1805, 2459, 2507, 2605
BtgI CCRYGG 3 cut(s) 71, 523, 2508
BtgZI GCGATG 2 cut(s) 1140, 2209
BtrI CACGTC 1 cut(s) 882
BtsCI GGATG 5 cut(s) 946, 2518, 2524, 2617, 2755
BtsI GCAGTG 1 cut(s) 1421
BtsIMutI CAGTG 5 cut(s) 337, 459, 1355, 1421, 2040
BveI ACCTGC 2 cut(s) 308, 2702
Cac8I GCNNGC 5 cut(s) 435, 818, 2133, 2661, 2729
CciI TCATGA 1 cut(s) 973
CfoI GCGC 3 cut(s) 242, 825, 1657
Cfr10I RCCGGY 2 cut(s) 25, 1761
Cfr13I GGNCC 8 cut(s) 88, 133, 339, 371, 646, 647, 1459, 1874
CpoI CGGWCCG 1 cut(s) 339
CseI GACGC 1 cut(s) 89
Csp6I GTAC 8 cut(s) 54, 301, 1577, 1751, 1759, 1927, 1963, 2485
CspAI ACCGGT 1 cut(s) 1761
CspI CGGWCCG 1 cut(s) 339
CviQI GTAC 8 cut(s) 54, 301, 1577, 1751, 1759, 1927, 1963, 2485
DraI TTTAAA 1 cut(s) 844
DrdI GACNNNNNNGTC 1 cut(s) 86
DseDI GACNNNNNNGTC 1 cut(s) 86
EaeI YGGCCR 3 cut(s) 74, 2457, 2505
Eam1104I CTCTTC 1 cut(s) 1173
EarI CTCTTC 1 cut(s) 1173
EciI GGCGGA 1 cut(s) 12
Ecl136II GAGCTC 2 cut(s) 918, 2502
Eco105I TACGTA 1 cut(s) 780
Eco130I CCWWGG 6 cut(s) 71, 523, 1068, 1523, 1979, 2508
Eco24I GRGCYC 4 cut(s) 650, 920, 2504, 2793
Eco31I GGTCTC 2 cut(s) 109, 1939
Eco32I GATATC 1 cut(s) 163
Eco47I GGWCC 6 cut(s) 88, 133, 339, 371, 1459, 1874
Eco53kI GAGCTC 2 cut(s) 918, 2502
Eco57I CTGAAG 3 cut(s) 281, 1745, 2064
Eco72I CACGTG 1 cut(s) 1649
EcoICRI GAGCTC 2 cut(s) 918, 2502
EcoO109I RGGNCCY 3 cut(s) 133, 647, 1459
EcoRII CCWGG 4 cut(s) 114, 135, 642, 1768
EcoRV GATATC 1 cut(s) 163
EcoT14I CCWWGG 6 cut(s) 71, 523, 1068, 1523, 1979, 2508
EcoT22I ATGCAT 1 cut(s) 999
EcoT38I GRGCYC 4 cut(s) 650, 920, 2504, 2793
ErhI CCWWGG 6 cut(s) 71, 523, 1068, 1523, 1979, 2508
FalI AAGNNNNNCTT 2 cut(s) 1963, 1995
FaqI GGGAC 5 cut(s) 748, 861, 1284, 1887, 2426
FauI CCCGC 1 cut(s) 87
FbaI TGATCA 2 cut(s) 250, 463
FokI GGATG 5 cut(s) 953, 2525, 2531, 2624, 2762
FriOI GRGCYC 4 cut(s) 650, 920, 2504, 2793
FspBI CTAG 8 cut(s) 506, 750, 1035, 1524, 1562, 2132, 2231, 2739
GlaI GCGC 3 cut(s) 241, 824, 1656
GsuI CTGGAG 1 cut(s) 435
HaeII RGCGCY 1 cut(s) 826
HaeIII GGCC 7 cut(s) 76, 522, 648, 1805, 2459, 2507, 2605
HapII CCGG 6 cut(s) 26, 77, 84, 91, 1277, 1762
HgaI GACGC 1 cut(s) 89
HhaI GCGC 3 cut(s) 242, 825, 1657
Hin1I GRCGYC 3 cut(s) 81, 985, 1113
Hin6I GCGC 3 cut(s) 240, 823, 1655
HinP1I GCGC 3 cut(s) 240, 823, 1655
HincII GTYRAC 2 cut(s) 1111, 1117
HindII GTYRAC 2 cut(s) 1111, 1117
HindIII AAGCTT 1 cut(s) 1530
HinfI GANTC 8 cut(s) 48, 626, 736, 1130, 1191, 1429, 1693, 1826
HpaII CCGG 6 cut(s) 26, 77, 84, 91, 1277, 1762
HphI GGTGA 5 cut(s) 369, 491, 879, 1931, 2534
Hpy166II GTNNAC 8 cut(s) 125, 1021, 1111, 1117, 1577, 1759, 1775, 1976
Hpy188I TCNGA 9 cut(s) 339, 741, 872, 922, 1201, 1315, 1322, 1474, 2402
Hpy8I GTNNAC 8 cut(s) 125, 1021, 1111, 1117, 1577, 1759, 1775, 1976
HpyCH4III ACNGT 7 cut(s) 366, 1121, 1142, 1350, 1437, 2484, 2597
HpyCH4IV ACGT 9 cut(s) 121, 779, 881, 985, 1113, 1648, 1879, 2184, 2473
HpyF10VI GCNNNNNNNGC 6 cut(s) 239, 323, 822, 1850, 2234, 2717
HpySE526I ACGT 9 cut(s) 121, 779, 881, 985, 1113, 1648, 1879, 2184, 2473
Hsp92I GRCGYC 3 cut(s) 81, 985, 1113
HspAI GCGC 3 cut(s) 240, 823, 1655
Kpn2I TCCGGA 1 cut(s) 1276
Ksp22I TGATCA 2 cut(s) 250, 463
LmnI GCTCC 5 cut(s) 45, 97, 311, 454, 1522
Lsp1109I GCAGC 7 cut(s) 358, 558, 938, 1639, 2129, 2249, 2524
LweI GCATC 4 cut(s) 913, 1612, 1831, 2224
MaeI CTAG 8 cut(s) 506, 750, 1035, 1524, 1562, 2132, 2231, 2739
MaeII ACGT 9 cut(s) 121, 779, 881, 985, 1113, 1648, 1879, 2184, 2473
MaeIII GTNAC 8 cut(s) 66, 200, 274, 440, 487, 1083, 1136, 2283
MflI RGATCY 5 cut(s) 502, 638, 1279, 1315, 2047
MhlI GDGCHC 7 cut(s) 127, 650, 920, 1519, 2504, 2595, 2793
MlsI TGGCCA 1 cut(s) 2459
MluCI AATT 8 cut(s) 540, 969, 1104, 1489, 1551, 1896, 2355, 2391
MluNI TGGCCA 1 cut(s) 2459
MlyI GAGTC 2 cut(s) 635, 730
MmeI TCCRAC 2 cut(s) 446, 1709
Mox20I TGGCCA 1 cut(s) 2459
Mph1103I ATGCAT 1 cut(s) 999
MroI TCCGGA 1 cut(s) 1276
MroXI GAANNNNTTC 1 cut(s) 1586
MscI TGGCCA 1 cut(s) 2459
MseI TTAA 9 cut(s) 141, 357, 539, 563, 591, 843, 1791, 2433, 2762
MslI CAYNNNNRTG 3 cut(s) 1179, 1572, 1811
Msp20I TGGCCA 1 cut(s) 2459
MspA1I CMGCKG 2 cut(s) 233, 433
MspI CCGG 6 cut(s) 26, 77, 84, 91, 1277, 1762
MspR9I CCNGG 5 cut(s) 85, 116, 137, 644, 1770
Mva1269I GAATGC 1 cut(s) 326
MvaI CCWGG 4 cut(s) 116, 137, 644, 1770
MvnI CGCG 1 cut(s) 240
MwoI GCNNNNNNNGC 6 cut(s) 239, 323, 822, 1850, 2234, 2717
NciI CCSGG 1 cut(s) 85
NcoI CCATGG 3 cut(s) 71, 523, 2508
NheI GCTAGC 1 cut(s) 2131
NlaIV GGNNCC 6 cut(s) 89, 648, 649, 1281, 1461, 1875
NmeAIII GCCGAG 2 cut(s) 153, 2483
NmuCI GTSAC 2 cut(s) 1083, 2283
NsiI ATGCAT 1 cut(s) 999
NspI RCATGY 2 cut(s) 1001, 2663
OliI CACNNNNGTG 1 cut(s) 1811
PaeI GCATGC 1 cut(s) 2663
PagI TCATGA 1 cut(s) 973
PasI CCCWGGG 1 cut(s) 643
PctI GAATGC 1 cut(s) 326
PdmI GAANNNNTTC 1 cut(s) 1586
PfeI GAWTC 6 cut(s) 48, 1130, 1191, 1429, 1693, 1826
PflFI GACNNNGTC 1 cut(s) 1025
PinAI ACCGGT 1 cut(s) 1761
PleI GAGTC 2 cut(s) 634, 730
PmaCI CACGTG 1 cut(s) 1649
PmlI CACGTG 1 cut(s) 1649
PpsI GAGTC 2 cut(s) 634, 730
Ppu21I YACGTR 3 cut(s) 780, 1649, 1880
PpuMI RGGWCCY 2 cut(s) 133, 1459
PshAI GACNNNNGTC 2 cut(s) 233, 1738
PshBI ATTAAT 2 cut(s) 357, 2433
PsiI TTATAA 4 cut(s) 1149, 1250, 1380, 1550
Psp124BI GAGCTC 2 cut(s) 920, 2504
Psp1406I AACGTT 1 cut(s) 2473
Psp5II RGGWCCY 2 cut(s) 133, 1459
Psp6I CCWGG 4 cut(s) 114, 135, 642, 1768
PspCI CACGTG 1 cut(s) 1649
PspGI CCWGG 4 cut(s) 114, 135, 642, 1768
PspN4I GGNNCC 6 cut(s) 89, 648, 649, 1281, 1461, 1875
PspOMI GGGCCC 1 cut(s) 646
PspPI GGNCC 8 cut(s) 88, 133, 339, 371, 646, 647, 1459, 1874
PspPPI RGGWCCY 2 cut(s) 133, 1459
PstI CTGCAG 1 cut(s) 1428
PsuI RGATCY 5 cut(s) 502, 638, 1279, 1315, 2047
PsyI GACNNNGTC 1 cut(s) 1025
PvuII CAGCTG 1 cut(s) 233
RsaI GTAC 8 cut(s) 55, 302, 1578, 1752, 1760, 1928, 1964, 2486
RsaNI GTAC 8 cut(s) 54, 301, 1577, 1751, 1759, 1927, 1963, 2485
RseI CAYNNNNRTG 3 cut(s) 1179, 1572, 1811
Rsr2I CGGWCCG 1 cut(s) 339
RsrII CGGWCCG 1 cut(s) 339
SacI GAGCTC 2 cut(s) 920, 2504
SaqAI TTAA 9 cut(s) 141, 357, 539, 563, 591, 843, 1791, 2433, 2762
Sau96I GGNCC 8 cut(s) 88, 133, 339, 371, 646, 647, 1459, 1874
SchI GAGTC 2 cut(s) 635, 730
ScrFI CCNGG 5 cut(s) 85, 116, 137, 644, 1770
SduI GDGCHC 7 cut(s) 127, 650, 920, 1519, 2504, 2595, 2793
SfaNI GCATC 4 cut(s) 913, 1612, 1831, 2224
SfcI CTRYAG 2 cut(s) 680, 1424
SgrAI CRCCGGYG 1 cut(s) 25
SinI GGWCC 6 cut(s) 88, 133, 339, 371, 1459, 1874
SmiMI CAYNNNNRTG 3 cut(s) 1179, 1572, 1811
SmlI CTYRAG 3 cut(s) 1292, 1326, 1936
SmoI CTYRAG 3 cut(s) 1292, 1326, 1936
SnaBI TACGTA 1 cut(s) 780
SphI GCATGC 1 cut(s) 2663
Sse9I AATT 8 cut(s) 540, 969, 1104, 1489, 1551, 1896, 2355, 2391
SspMI CTAG 8 cut(s) 506, 750, 1035, 1524, 1562, 2132, 2231, 2739
SstI GAGCTC 2 cut(s) 920, 2504
StyD4I CCNGG 5 cut(s) 83, 114, 135, 642, 1768
StyI CCWWGG 6 cut(s) 71, 523, 1068, 1523, 1979, 2508
TaaI ACNGT 7 cut(s) 366, 1121, 1142, 1350, 1437, 2484, 2597
TaiI ACGT 9 cut(s) 124, 782, 884, 988, 1116, 1651, 1882, 2187, 2476
TaqI TCGA 3 cut(s) 227, 1432, 2342
TaqII GACCGA 1 cut(s) 356
TasI AATT 8 cut(s) 540, 969, 1104, 1489, 1551, 1896, 2355, 2391
TatI WGTACW 3 cut(s) 53, 300, 1926
TauI GCSGC 3 cut(s) 823, 1356, 1408
TfiI GAWTC 6 cut(s) 48, 1130, 1191, 1429, 1693, 1826
Tru1I TTAA 9 cut(s) 141, 357, 539, 563, 591, 843, 1791, 2433, 2762
Tru9I TTAA 9 cut(s) 141, 357, 539, 563, 591, 843, 1791, 2433, 2762
TscAI CASTG 5 cut(s) 337, 459, 1355, 1428, 2047
TseFI GTSAC 2 cut(s) 1083, 2283
TseI GCWGC 7 cut(s) 346, 546, 926, 1652, 2117, 2237, 2537
Tsp45I GTSAC 2 cut(s) 1083, 2283
TspGWI ACGGA 3 cut(s) 32, 188, 1746
TspRI CASTG 5 cut(s) 337, 459, 1355, 1428, 2047
Tth111I GACNNNGTC 1 cut(s) 1025
VneI GTGCAC 1 cut(s) 123
VpaK11BI GGWCC 6 cut(s) 88, 133, 339, 371, 1459, 1874
VspI ATTAAT 2 cut(s) 357, 2433
XapI RAATTY 1 cut(s) 2355
XbaI TCTAGA 2 cut(s) 505, 2230
XceI RCATGY 2 cut(s) 1001, 2663
XcmI CCANNNNNNNNNTGG 2 cut(s) 1492, 1977
XmaJI CCTAGG 1 cut(s) 1523
XmnI GAANNNNTTC 1 cut(s) 1586
XspI CTAG 8 cut(s) 506, 750, 1035, 1524, 1562, 2132, 2231, 2739
ZraI GACGTC 2 cut(s) 986, 1114
Zsp2I ATGCAT 1 cut(s) 999
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.