Rh2CG065700
ERF Family

Probably involved in the RNA silencing pathway and required for the generation of small interfering RNAs (siRNAs)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0000505
Physical Location & Seq
Forward (+)
5471971 .. 5485717
13747 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG065700.1

Sequence Viewer

Length: 3327 bp
ATGTCCGGTGTGCAGCTACTCGACTCCGTCCAGCAATCGCTGGATCGAATATGCGACCTTAAGAACCAACCACAGCCGGACGACGGCATCAGAAGAAAGCTGGCATTGCTGAGCGAGCAAGACGCCCTCCACGTGCTAAACCGAATTTACAACTACAAAACTCCCATCAGAACCCTCGATGGATTGATCACTTTCATGATCGGGAAACTAGAGAATGGATCTTCTTCATCTTCTTCGAGAACAGTTTGGACCCCTCAGAGTCCATCTGCTTCTTCTTCTTCTGTTAGTAGTCCTGCAAGGCCTCATCATTCTCAAGTTGTAGACCAGTCTGGAGAGAATGGACTCTGTTCCTCTGTTTCTCAACGGCTATATTTAGTTGCATGGTTTTTCAATTTGGTCAATTGCTGTTTCATGAAATTCCTAAATGAAGCCGATTGTGTTAATACGAGTGAGAGCTTTGTTGCAGGTGAGACAATCCAGTGTGGTTCTCCTGTTAATCGACAGCCACTCTATTCTTCAGCTTCTCCTTCTCAAGCTTCCCCGACTTCTTCTTATCAGAGCCATTCCTCTCCTACCATTACTGATGCATCATTTTATTCCAGTTCTCAGCCTCAGGGTGAGACTCCCAACCCATCCCTGGGGACAGTGTGCAATGGAAAATCACCCTATTCCTTCGCTTCTCTCTATCAAATCCTATCTGCTCCTTTTAATACTCTTAGACAACTCTATGAGAGGTTCCGAGGTGAGACTGTCACTCACTCCCCAGTTCTATACCATCCTGGAGACCATGGACCAGCACACTTAGAAGCTCTGGGAGAACTCGATTTCAGAAAGCAATTTTTGATTTTGAGTTATGCTGGAGGAACGAAGTTAAAAGATGTAATAGAAGCGGAGGCTATTAGGAGCTGGAAAAGTTTGCCAATGGTAAAGTTCGAGACCACAGTTTGGCATGCTTTGGGTGAAAACAATGTTGGCAAGGCTGACCGACGATTGGGTCCCTTCCTAGACAAACCAAGAACTCTCCTACAGAAACAGTTAGGAGATGACAATGTTTTGGAGGTGAAATTTACCCAAGGGGAAGGTTCCAGTGGTACATATAGCAAGTTGATACGAGAAGGCATTCTTGTTGGTTTGCGGCGATACAGTTTCTTTGTGTTCCGTGCTGGTAACAAAGGAAAGAATATGTGGAAAGACCCAACTTCAACACCTGTGAAATGTTACTTTGTTCGTGGGAGCTCTGATGCAGTGATAGACAGGGGTATAGGCTATAAGTTATCTGGCAAAACGATGCATGAAGGAAGATCTCTTTTCACGCATGCACACACTGGGCCTAGTTTGGCTAGCTACATGGATAGGTTATCTCTCATTTTGTCCAAGACAGTTACTTTAGAAGTAAATTGGTCACATGTCAAGGTGGAATTCATCAAAGACGAAGACTGTCGCGATGAGTCTGGTAATCCCATCTATGTAGATGGAAAACCTCGTATACATACTGATGGCACAGGATTTATATCTGAGGATCTGGCTTTACTTTGTCCTGAGATAGCACGCAAAGGAGAGCAGATCAATAATGAACACATTGAGGGACTTGATGATCCTGATGATGAACTTGAGGACAAAATTCTGGAATGGAAACAGCCAGGCACTAGAACTCGGGAACTGCCTTTGCTCATGCAGTTCCGACTTTTTTACAAAGGCCGTGCTATCAAAGGAACTTTTCTTGTCAATAAAGAGCTTCCACCAAAAACTTTGAAGATTCGACCATCAATGGTTAAAGTTGAGCCAGATCCAAAATTGTCAGATCCTCAAACAGTGAATTCACTGGAGATAGTAGGAACAAGTAAACTTAATAAGAGAACATATTTGTCGAGGAACTTAATTGCACTTTTGTACCATGGAGGGGTGCCAAAAAAATACTTCATCGAGTTGCTTATGAAAGCTTTAGAAGAGACTCGTGATGTTTTTAGCAATCCACGCGCAGCATTCAGAGTTGCCCTTAGATATGGCGAGATCGATGACAACAGCACTGTTGCGAAAATGATCTCCTGTGGTATTCCTCTTGAAGAACCATATGTGCAATATCGTCTTTCAACCCTTATGAACATGGAAAAGATCAATCTCAAAAAAGGGAAAATTTGTATTCCTGAATCATACTATTTGATGGGGACAACTGATCCAACAGGAAAACTTGAAAAAGATGAAGTTTGCATTATCCTAGAAAATGGACAACTACTGTCGGGAGATTTTCTAGTGTACCGGAATCCTGGTTTACACTTTGGTGATATTCATGTCCTAAAGGCCAGATATGTCAAGGAACTAGAATCTGTTGTTGGCAATGCCAAGTACGGTATATTCTTCTCTAGTAAAGGTCCACGTTCCGTTGCTGATGAAATGGCTGGTGGAGATTTTGATGGTGATCTATACTGGATCTCAAGCAACCCTCAGCTATTAAAATCGTTTAAACCAAGTGAACCTTGGATTGGAAAGTCATCTGCCCAAAAAGTTCATGGTAAAAGACCAACCGAGCTTTCAAAGGATGATCTAGAAGATGAGCTCATCAAACTATTCTTGAAGACTAGGTTTGAACCAAGCTTCGCAGTGTATGAGGCAGCTCAAAACTGGATGGCTTTGATGGATAAATTTTTGACTCTAGGAGATACGAGTATTGATGTGAAAAACAAACTGAAGGATAAGATACTGCGATTGATTGATATTTACTATGAAGCTCTCGATGCACCAAAAAAAGGTTTTGAGGTTGTAGTTCCAAAAGAATTGAAGGTGCGAGAGTTCCCACATTTCATGGAGAAGAAAAACCAAGAATCCTACCATTCAAGTTCCATTTTGGGATCCATTTATGATAAGGTGGAGGAATATGAAACAGAAGACAATCTGAAAGAAATTCGGAAACTTCCTCTTTTTGAAGACGAGTACGTTCCTGAGGACCGCTTGAAGGAATGGGAGAAACGATACGAAGAGTACCGGAAGAAGATGACCTCTGCCATGGGAAATGAAGATAAAGAGAGCAAGAATCAGGCAGCCAATATCGTCATAAGAGAGTATAAAGAGATCCTATACGAGGCTGAGGAGTTTGAGGAAAGTAAAAGGGAAAGACAACAAATATATAACGACGCACTCGCTATTTATCATGTTTGTTATGACTATGCCAGGAGTGTGAGGAATGAAACTGGGGCACAAGATGTCCGGAAATGTGGCTTTGTCTGGAAAGTTGCAGGTGCAGCTCTATGCCAGCTTCATGAGGACCGGAGTTTGAAACCTGGTGAAAAGGTTTTCAGGATCTTACCCTCTGTTTTGAAGGATATTATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RdRP

Protein Analysis

1108

Amino Acids

125.38

Weight (kDa)

6.68

Isoelectric Point (pI)

38.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
4HB_RdRP3_N PF26249 6 - 67 7.1e-08 RdRP3/4/5, N-terminal four helical bundle domain
RdRP_helical PF26252 267 - 331 4.7e-08 RNA dependent RNA polymerase, helical domain
RdRP PF05183 339 - 955 6.9e-101 RNA dependent RNA polymerase
RdRP_head PF26253 972 - 1087 6.8e-13 RdRP, head domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000505)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G19910 AT2G19910 AT2G19920 AT2G19920 AT2G19920 AT2G19920 AT2G19930 AT2G19930 AT2G19930
fragaria_vesca FvH4_1g05980 FvH4_1g05990 FvH4_1g05990 FvH4_1g09718
malus_domestica MD02G1064400.v1.1 MD15G1195300.v1.1 MD15G1195400.v1.1
prunus_persica Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1
pyrus_communis pycom02g05070 pycom15g17460
rosa_chinensis RchiOBHm_Chr2g0091671 RchiOBHm_Chr2g0091681 RchiOBHm_Chr2g0091691 RchiOBHm_Chr2g0091701 RchiOBHm_Chr2g0091711 RchiOBHm_Chr2g0091721 RchiOBHm_Chr2g0091731 RchiOBHm_Chr5g0014401
rosa_laevigata RLG00000013772 RLG00000016242 RLG00000016244 RLG00000016245 RLG00000016246 RLG00000032105
rosa_multiflora Rmu_co8374041.1_g000001 Rmu_co8478121.1_g000001 Rmu_sc0000897.1_g000005 Rmu_sc0000897.1_g000009
rosa_roxburghii Rroxscaffold_1G00062180 Rroxscaffold_1G00062190 Rroxscaffold_1G00068800 Rroxscaffold_2G00149790 Rroxscaffold_2G00149800 Rroxscaffold_2G00149810
rosa_rugosa Rorug02G0019600 Rorug02G0019700 Rorug02G0019800 Rorug02G0019900 Rorug02G0019900 Rorug02G0020000 Rorug02G0020000 Rorug02G0020100 Rorug02G0020100 Rorug02G0020200 Rorug05G0017800
rosa_samantha Rh2AG064700 Rh2AG064800 Rh2AG064900 Rh2BG063800 Rh2BG063900 Rh2BG064000 Rh2BG064100 Rh2CG065500 Rh2CG065600 Rh2CG065700 Rh2DG063900 Rh2DG064000 Rh2DG064100 Rh2DG064200 Rh2DG381700 Rh5AG056700 Rh5AG056800 Rh5AG056900 Rh5AG112600 Rh5BG109300 Rh5CG063800 Rh5CG121100 Rh5DG053100 Rh5DG108300 Rh6CG100100
rosa_wichuraiana Rw2G005560 Rw2G005580 Rw2G005590 Rw5G005370 Rw5G009770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 455, 3221
AasI GACNNNNNNGTC 1 cut(s) 993
Acc36I ACCTGC 2 cut(s) 455, 3221
AccB1I GGYRCC 1 cut(s) 1903
AccB7I CCANNNNNTGG 1 cut(s) 945
AccI GTMKAC 2 cut(s) 321, 1486
AccII CGCG 2 cut(s) 1443, 1977
AccIII TCCGGA 1 cut(s) 3201
AciI CCGC 3 cut(s) 890, 1135, 2944
AcsI RAATTY 9 cut(s) 144, 416, 1064, 1418, 1620, 1816, 2133, 2639, 2898
AcuI CTGAAG 2 cut(s) 501, 2705
AcvI CACGTG 1 cut(s) 133
AcyI GRCGYC 1 cut(s) 123
AfaI GTAC 6 cut(s) 1093, 1892, 2255, 2345, 2930, 2978
AflII CTTAAG 1 cut(s) 59
AflIII ACRYGT 1 cut(s) 1405
AjnI CCWGG 6 cut(s) 636, 778, 1639, 2263, 3164, 3274
AjuI GAANNNNNNNTTGG 8 cut(s) 954, 986, 2313, 2345, 2463, 2495, 2512, 2544
AleI CACNNNNGTG 1 cut(s) 2277
Alw21I GWGCWC 2 cut(s) 1238, 2556
Alw26I GTCTC 6 cut(s) 464, 614, 740, 777, 929, 1943
Ama87I CYCGRG 1 cut(s) 1653
Aor13HI TCCGGA 1 cut(s) 3201
AoxI GGCC 4 cut(s) 299, 1328, 1696, 2298
ApeKI GCWGC 5 cut(s) 13, 1979, 2609, 3035, 3236
ApoI RAATTY 9 cut(s) 144, 416, 1064, 1418, 1620, 1816, 2133, 2639, 2898
Asp700I GAANNNNTTC 1 cut(s) 1119
AspLEI GCGC 1 cut(s) 1979
AspS9I GGNCC 7 cut(s) 249, 791, 995, 1328, 2369, 2941, 3259
AsuHPI GGTGA 9 cut(s) 479, 629, 654, 755, 971, 1072, 2291, 2426, 3290
AsuNHI GCTAGC 1 cut(s) 1340
AvaI CYCGRG 1 cut(s) 1653
AvaII GGWCC 6 cut(s) 249, 791, 995, 2369, 2941, 3259
AxyI CCTNAGG 2 cut(s) 612, 2937
BaeGI GKGCMC 1 cut(s) 3193
BaeI ACNNNNGTAYC 2 cut(s) 1874, 1907
BamHI GGATCC 1 cut(s) 2846
BanI GGYRCC 1 cut(s) 1903
BanII GRGCYC 2 cut(s) 1238, 2556
BauI CACGAG 1 cut(s) 1953
BbrPI CACGTG 1 cut(s) 133
BbsI GAAGAC 4 cut(s) 1440, 2579, 2889, 2928
Bbv12I GWGCWC 2 cut(s) 1238, 2556
BbvCI CCTCAGC 2 cut(s) 2442, 3081
BbvI GCAGC 5 cut(s) 25, 1991, 2621, 3047, 3248
BceAI ACGGC 3 cut(s) 100, 380, 1683
BciT130I CCWGG 6 cut(s) 638, 780, 1641, 2265, 3166, 3276
BclI TGATCA 1 cut(s) 186
BcoDI GTCTC 6 cut(s) 464, 614, 740, 777, 929, 1943
BfmI CTRYAG 1 cut(s) 1025
BfrI CTTAAG 1 cut(s) 59
BfuAI ACCTGC 2 cut(s) 455, 3221
BglII AGATCT 1 cut(s) 1301
BisI GCNGC 6 cut(s) 14, 1136, 1980, 2610, 3036, 3237
BlpI GCTNAGC 1 cut(s) 110
BlsI GCNGC 6 cut(s) 15, 1137, 1981, 2611, 3037, 3238
Bme1390I CCNGG 6 cut(s) 638, 780, 1641, 2265, 3166, 3276
Bme18I GGWCC 6 cut(s) 249, 791, 995, 2369, 2941, 3259
BmeT110I CYCGRG 1 cut(s) 1653
BmgT120I GGNCC 7 cut(s) 249, 791, 995, 1328, 2369, 2941, 3259
BmiI GGNNCC 7 cut(s) 251, 737, 996, 997, 1084, 1905, 2848
BmrFI CCNGG 6 cut(s) 638, 780, 1641, 2265, 3166, 3276
BmrI ACTGGG 3 cut(s) 758, 1335, 3195
BmsI GCATC 6 cut(s) 96, 574, 596, 1231, 1278, 2722
BmtI GCTAGC 1 cut(s) 1344
BmuI ACTGGG 3 cut(s) 758, 1335, 3195
BpiI GAAGAC 4 cut(s) 1440, 2579, 2889, 2928
BpmI CTGGAG 4 cut(s) 351, 801, 879, 1844
Bpu10I CCTNAGC 2 cut(s) 2442, 3081
Bpu1102I GCTNAGC 1 cut(s) 110
BpuEI CTTGAG 4 cut(s) 297, 516, 1631, 2416
Bsa29I ATCGAT 1 cut(s) 2013
BsaAI YACGTR 1 cut(s) 133
BsaBI GATNNNNATC 2 cut(s) 1510, 2415
BsaHI GRCGYC 1 cut(s) 123
BsaI GGTCTC 2 cut(s) 777, 929
BsaJI CCNNGG 8 cut(s) 636, 637, 739, 787, 1072, 1894, 2474, 3000
BsaWI WCCGGW 5 cut(s) 5, 2256, 2979, 3201, 3261
Bse21I CCTNAGG 2 cut(s) 612, 2937
Bse3DI GCAATG 3 cut(s) 104, 658, 2341
Bse8I GATNNNNATC 2 cut(s) 1510, 2415
BseAI TCCGGA 1 cut(s) 3201
BseBI CCWGG 6 cut(s) 638, 780, 1641, 2265, 3166, 3276
BseCI ATCGAT 1 cut(s) 2013
BseDI CCNNGG 8 cut(s) 636, 637, 739, 787, 1072, 1894, 2474, 3000
BseGI GGATG 4 cut(s) 632, 775, 2542, 2628
BseJI GATNNNNATC 2 cut(s) 1510, 2415
BseMI GCAATG 3 cut(s) 104, 658, 2341
BseMII CTCAG 9 cut(s) 101, 269, 620, 626, 1506, 1530, 2456, 2928, 3072
BseRI GAGGAG 1 cut(s) 3098
BseSI GKGCMC 1 cut(s) 3193
BseXI GCAGC 5 cut(s) 25, 1991, 2621, 3047, 3248
BsgI GTGCAG 2 cut(s) 32, 3255
Bsh1236I CGCG 2 cut(s) 1443, 1977
BshFI GGCC 4 cut(s) 301, 1330, 1698, 2300
BshNI GGYRCC 1 cut(s) 1903
BshVI ATCGAT 1 cut(s) 2013
BsiHKAI GWGCWC 2 cut(s) 1238, 2556
BsiHKCI CYCGRG 1 cut(s) 1653
BsiSI CCGG 6 cut(s) 6, 77, 2257, 2980, 3202, 3262
BslFI GGGAC 4 cut(s) 655, 981, 1599, 2179
BsmAI GTCTC 6 cut(s) 464, 614, 740, 777, 929, 1943
BsmFI GGGAC 4 cut(s) 655, 981, 1599, 2179
BsmI GAATGC 2 cut(s) 1119, 1982
BsnI GGCC 4 cut(s) 301, 1330, 1698, 2300
Bso31I GGTCTC 2 cut(s) 777, 929
BsoBI CYCGRG 1 cut(s) 1653
Bsp1286I GDGCHC 3 cut(s) 1238, 2556, 3193
Bsp13I TCCGGA 1 cut(s) 3201
Bsp1720I GCTNAGC 1 cut(s) 110
Bsp19I CCATGG 3 cut(s) 787, 1894, 3000
Bsp68I TCGCGA 1 cut(s) 1443
BspACI CCGC 3 cut(s) 890, 1135, 2944
BspANI GGCC 4 cut(s) 301, 1330, 1698, 2300
BspCNI CTCAG 9 cut(s) 102, 268, 619, 625, 1507, 1531, 2455, 2929, 3073
BspDI ATCGAT 1 cut(s) 2013
BspEI TCCGGA 1 cut(s) 3201
BspFNI CGCG 2 cut(s) 1443, 1977
BspHI TCATGA 3 cut(s) 195, 411, 3253
BspLI GGNNCC 7 cut(s) 251, 737, 996, 997, 1084, 1905, 2848
BspMI ACCTGC 2 cut(s) 455, 3221
BspOI GCTAGC 1 cut(s) 1344
BspT107I GGYRCC 1 cut(s) 1903
BspTI CTTAAG 1 cut(s) 59
BspTNI GGTCTC 2 cut(s) 777, 929
BsrDI GCAATG 3 cut(s) 104, 658, 2341
BssECI CCNNGG 8 cut(s) 636, 637, 739, 787, 1072, 1894, 2474, 3000
BssNAI GTATAC 1 cut(s) 1487
BssNI GRCGYC 1 cut(s) 123
BssSI CACGAG 1 cut(s) 1953
BssT1I CCWWGG 5 cut(s) 787, 1072, 1894, 2474, 3000
Bst1107I GTATAC 1 cut(s) 1487
Bst2BI CACGAG 1 cut(s) 1953
Bst2UI CCWGG 6 cut(s) 638, 780, 1641, 2265, 3166, 3276
Bst6I CTCTTC 2 cut(s) 1941, 2967
BstACI GRCGYC 1 cut(s) 123
BstAFI CTTAAG 1 cut(s) 59
BstBAI YACGTR 1 cut(s) 133
BstC8I GCNNGC 7 cut(s) 102, 116, 951, 1317, 1342, 1549, 3248
BstDSI CCRYGG 3 cut(s) 787, 1894, 3000
BstENI CCTNNNNNAGG 1 cut(s) 3074
BstF5I GGATG 4 cut(s) 632, 775, 2542, 2628
BstFNI CGCG 2 cut(s) 1443, 1977
BstHHI GCGC 1 cut(s) 1979
BstMAI GTCTC 6 cut(s) 464, 614, 740, 777, 929, 1943
BstMWI GCNNNNNNNGC 5 cut(s) 106, 115, 1974, 2732, 3236
BstNI CCWGG 6 cut(s) 638, 780, 1641, 2265, 3166, 3276
BstNSI RCATGY 3 cut(s) 953, 1319, 1409
BstSCI CCNGG 6 cut(s) 636, 778, 1639, 2263, 3164, 3274
BstSFI CTRYAG 1 cut(s) 1025
BstSLI GKGCMC 1 cut(s) 3193
BstUI CGCG 2 cut(s) 1443, 1977
BstV1I GCAGC 5 cut(s) 25, 1991, 2621, 3047, 3248
BstV2I GAAGAC 4 cut(s) 1440, 2579, 2889, 2928
BstX2I RGATCY 9 cut(s) 218, 1301, 1519, 1786, 1801, 2427, 2846, 3066, 3294
BstYI RGATCY 9 cut(s) 218, 1301, 1519, 1786, 1801, 2427, 2846, 3066, 3294
BstZ17I GTATAC 1 cut(s) 1487
Bsu15I ATCGAT 1 cut(s) 2013
Bsu36I CCTNAGG 2 cut(s) 612, 2937
BsuRI GGCC 4 cut(s) 301, 1330, 1698, 2300
BsuTUI ATCGAT 1 cut(s) 2013
BtgI CCRYGG 3 cut(s) 787, 1894, 3000
BtgZI GCGATG 1 cut(s) 1458
BtsCI GGATG 4 cut(s) 632, 775, 2542, 2628
BtsI GCAGTG 2 cut(s) 1251, 2604
BtsIMutI CAGTG 9 cut(s) 485, 651, 1093, 1251, 1323, 1818, 1820, 2025, 2604
BtuMI TCGCGA 1 cut(s) 1443
BveI ACCTGC 2 cut(s) 455, 3221
Cac8I GCNNGC 7 cut(s) 102, 116, 951, 1317, 1342, 1549, 3248
CciI TCATGA 3 cut(s) 195, 411, 3253
CfoI GCGC 1 cut(s) 1979
Cfr13I GGNCC 7 cut(s) 249, 791, 995, 1328, 2369, 2941, 3259
ClaI ATCGAT 1 cut(s) 2013
CseI GACGC 2 cut(s) 131, 3137
CsiI ACCWGGT 1 cut(s) 3274
Csp6I GTAC 6 cut(s) 1092, 1891, 2254, 2344, 2929, 2977
CviQI GTAC 6 cut(s) 1092, 1891, 2254, 2344, 2929, 2977
DraI TTTAAA 1 cut(s) 2461
DrdI GACNNNNNNGTC 1 cut(s) 993
DseDI GACNNNNNNGTC 1 cut(s) 993
Eam1104I CTCTTC 2 cut(s) 1941, 2967
EarI CTCTTC 2 cut(s) 1941, 2967
Ecl136II GAGCTC 2 cut(s) 1236, 2554
Eco130I CCWWGG 5 cut(s) 787, 1072, 1894, 2474, 3000
Eco147I AGGCCT 1 cut(s) 301
Eco24I GRGCYC 2 cut(s) 1238, 2556
Eco31I GGTCTC 2 cut(s) 777, 929
Eco47I GGWCC 6 cut(s) 249, 791, 995, 2369, 2941, 3259
Eco53kI GAGCTC 2 cut(s) 1236, 2554
Eco57I CTGAAG 2 cut(s) 501, 2705
Eco72I CACGTG 1 cut(s) 133
Eco81I CCTNAGG 2 cut(s) 612, 2937
Eco88I CYCGRG 1 cut(s) 1653
EcoICRI GAGCTC 2 cut(s) 1236, 2554
EcoNI CCTNNNNNAGG 1 cut(s) 3074
EcoO109I RGGNCCY 1 cut(s) 995
EcoRI GAATTC 2 cut(s) 1418, 1816
EcoRII CCWGG 6 cut(s) 636, 778, 1639, 2263, 3164, 3274
EcoT14I CCWWGG 5 cut(s) 787, 1072, 1894, 2474, 3000
EcoT22I ATGCAT 2 cut(s) 589, 1293
EcoT38I GRGCYC 2 cut(s) 1238, 2556
ErhI CCWWGG 5 cut(s) 787, 1072, 1894, 2474, 3000
FalI AAGNNNNNCTT 4 cut(s) 1107, 1139, 2458, 2490
FaqI GGGAC 4 cut(s) 655, 981, 1599, 2179
FauNDI CATATG 1 cut(s) 2071
FbaI TGATCA 1 cut(s) 186
FblI GTMKAC 2 cut(s) 321, 1486
Fnu4HI GCNGC 6 cut(s) 14, 1136, 1980, 2610, 3036, 3237
FokI GGATG 4 cut(s) 619, 762, 2549, 2635
FriOI GRGCYC 2 cut(s) 1238, 2556
Fsp4HI GCNGC 6 cut(s) 14, 1136, 1980, 2610, 3036, 3237
GlaI GCGC 1 cut(s) 1978
GluI GCNGC 6 cut(s) 14, 1136, 1980, 2610, 3036, 3237
GsuI CTGGAG 4 cut(s) 351, 801, 879, 1844
HaeIII GGCC 4 cut(s) 301, 1330, 1698, 2300
HapII CCGG 6 cut(s) 6, 77, 2257, 2980, 3202, 3262
HgaI GACGC 2 cut(s) 131, 3137
HhaI GCGC 1 cut(s) 1979
Hin1I GRCGYC 1 cut(s) 123
Hin6I GCGC 1 cut(s) 1977
HinP1I GCGC 1 cut(s) 1977
HindIII AAGCTT 3 cut(s) 534, 1938, 2590
HpaII CCGG 6 cut(s) 6, 77, 2257, 2980, 3202, 3262
HphI GGTGA 9 cut(s) 479, 629, 654, 755, 971, 1072, 2291, 2426, 3290
Hpy166II GTNNAC 7 cut(s) 322, 1487, 1844, 2254, 2270, 2372, 2471
Hpy8I GTNNAC 7 cut(s) 322, 1487, 1844, 2254, 2270, 2372, 2471
Hpy99I CGWCG 3 cut(s) 86, 990, 3131
HpyCH4IV ACGT 3 cut(s) 132, 2374, 2931
HpyF10VI GCNNNNNNNGC 5 cut(s) 106, 115, 1974, 2732, 3236
HpySE526I ACGT 3 cut(s) 132, 2374, 2931
Hsp92I GRCGYC 1 cut(s) 123
HspAI GCGC 1 cut(s) 1977
KflI GGGWCCC 1 cut(s) 995
Kpn2I TCCGGA 1 cut(s) 3201
Ksp22I TGATCA 1 cut(s) 186
LmnI GCTCC 3 cut(s) 706, 903, 1233
Lsp1109I GCAGC 5 cut(s) 25, 1991, 2621, 3047, 3248
LweI GCATC 6 cut(s) 96, 574, 596, 1231, 1278, 2722
MabI ACCWGGT 1 cut(s) 3274
MaeII ACGT 3 cut(s) 132, 2374, 2931
MaeIII GTNAC 5 cut(s) 751, 1166, 1217, 1381, 1401
MfeI CAATTG 1 cut(s) 400
MflI RGATCY 9 cut(s) 218, 1301, 1519, 1786, 1801, 2427, 2846, 3066, 3294
MhlI GDGCHC 3 cut(s) 1238, 2556, 3193
MlyI GAGTC 7 cut(s) 17, 268, 336, 616, 1457, 1945, 2641
MmeI TCCRAC 2 cut(s) 1705, 2201
Mph1103I ATGCAT 2 cut(s) 589, 1293
MroI TCCGGA 1 cut(s) 3201
MroXI GAANNNNTTC 1 cut(s) 1119
MslI CAYNNNNRTG 3 cut(s) 194, 1494, 2277
MspCI CTTAAG 1 cut(s) 59
MspI CCGG 6 cut(s) 6, 77, 2257, 2980, 3202, 3262
MspR9I CCNGG 6 cut(s) 638, 780, 1641, 2265, 3166, 3276
MssI GTTTAAAC 1 cut(s) 2461
MunI CAATTG 1 cut(s) 400
Mva1269I GAATGC 2 cut(s) 1119, 1982
MvaI CCWGG 6 cut(s) 638, 780, 1641, 2265, 3166, 3276
MvnI CGCG 2 cut(s) 1443, 1977
MwoI GCNNNNNNNGC 5 cut(s) 106, 115, 1974, 2732, 3236
NcoI CCATGG 3 cut(s) 787, 1894, 3000
NdeI CATATG 1 cut(s) 2071
NheI GCTAGC 1 cut(s) 1340
NlaIV GGNNCC 7 cut(s) 251, 737, 996, 997, 1084, 1905, 2848
NmuCI GTSAC 2 cut(s) 751, 1401
NruI TCGCGA 1 cut(s) 1443
NsiI ATGCAT 2 cut(s) 589, 1293
NspI RCATGY 3 cut(s) 953, 1319, 1409
OliI CACNNNNGTG 1 cut(s) 2277
PaeI GCATGC 2 cut(s) 953, 1319
PagI TCATGA 3 cut(s) 195, 411, 3253
PaqCI CACCTGC 2 cut(s) 455, 3221
PasI CCCWGGG 1 cut(s) 637
PceI AGGCCT 1 cut(s) 301
PciI ACATGT 1 cut(s) 1405
PcsI WCGNNNNNNNCGW 1 cut(s) 129
PctI GAATGC 2 cut(s) 1119, 1982
PdmI GAANNNNTTC 1 cut(s) 1119
PfeI GAWTC 6 cut(s) 1756, 2147, 2260, 2321, 2819, 3028
PflFI GACNNNGTC 1 cut(s) 26
PflMI CCANNNNNTGG 1 cut(s) 945
PfoI TCCNGGA 1 cut(s) 778
PkrI GCNGC 6 cut(s) 15, 1137, 1981, 2611, 3037, 3238
PleI GAGTC 7 cut(s) 17, 267, 336, 616, 1456, 1945, 2641
PmaCI CACGTG 1 cut(s) 133
PmeI GTTTAAAC 1 cut(s) 2461
PmlI CACGTG 1 cut(s) 133
PpsI GAGTC 7 cut(s) 17, 267, 336, 616, 1456, 1945, 2641
Ppu21I YACGTR 1 cut(s) 133
PpuMI RGGWCCY 1 cut(s) 995
PscI ACATGT 1 cut(s) 1405
Psp124BI GAGCTC 2 cut(s) 1238, 2556
Psp5II RGGWCCY 1 cut(s) 995
Psp6I CCWGG 6 cut(s) 636, 778, 1639, 2263, 3164, 3274
PspCI CACGTG 1 cut(s) 133
PspGI CCWGG 6 cut(s) 636, 778, 1639, 2263, 3164, 3274
PspN4I GGNNCC 7 cut(s) 251, 737, 996, 997, 1084, 1905, 2848
PspPI GGNCC 7 cut(s) 249, 791, 995, 1328, 2369, 2941, 3259
PspPPI RGGWCCY 1 cut(s) 995
PsuI RGATCY 9 cut(s) 218, 1301, 1519, 1786, 1801, 2427, 2846, 3066, 3294
PsyI GACNNNGTC 1 cut(s) 26
RruI TCGCGA 1 cut(s) 1443
RsaI GTAC 6 cut(s) 1093, 1892, 2255, 2345, 2930, 2978
RsaNI GTAC 6 cut(s) 1092, 1891, 2254, 2344, 2929, 2977
RseI CAYNNNNRTG 3 cut(s) 194, 1494, 2277
SacI GAGCTC 2 cut(s) 1238, 2556
SatI GCNGC 6 cut(s) 14, 1136, 1980, 2610, 3036, 3237
Sau96I GGNCC 7 cut(s) 249, 791, 995, 1328, 2369, 2941, 3259
SchI GAGTC 7 cut(s) 17, 268, 336, 616, 1457, 1945, 2641
ScrFI CCNGG 6 cut(s) 638, 780, 1641, 2265, 3166, 3276
SduI GDGCHC 3 cut(s) 1238, 2556, 3193
SexAI ACCWGGT 1 cut(s) 3274
SfaNI GCATC 6 cut(s) 96, 574, 596, 1231, 1278, 2722
SfcI CTRYAG 1 cut(s) 1025
SinI GGWCC 6 cut(s) 249, 791, 995, 2369, 2941, 3259
SmiMI CAYNNNNRTG 3 cut(s) 194, 1494, 2277
SmlI CTYRAG 5 cut(s) 59, 312, 531, 1610, 2431
SmoI CTYRAG 5 cut(s) 59, 312, 531, 1610, 2431
SphI GCATGC 2 cut(s) 953, 1319
SseBI AGGCCT 1 cut(s) 301
SsiI CCGC 3 cut(s) 890, 1135, 2944
SstI GAGCTC 2 cut(s) 1238, 2556
StuI AGGCCT 1 cut(s) 301
StyD4I CCNGG 6 cut(s) 636, 778, 1639, 2263, 3164, 3274
StyI CCWWGG 5 cut(s) 787, 1072, 1894, 2474, 3000
TaiI ACGT 3 cut(s) 135, 2377, 2934
TaqII GACCGA 1 cut(s) 999
TauI GCSGC 1 cut(s) 1138
TfiI GAWTC 6 cut(s) 1756, 2147, 2260, 2321, 2819, 3028
TscAI CASTG 9 cut(s) 485, 651, 1093, 1251, 1330, 1818, 1827, 2032, 2604
TseFI GTSAC 2 cut(s) 751, 1401
TseI GCWGC 5 cut(s) 13, 1979, 2609, 3035, 3236
Tsp45I GTSAC 2 cut(s) 751, 1401
TspGWI ACGGA 3 cut(s) 16, 1148, 2368
TspRI CASTG 9 cut(s) 485, 651, 1093, 1251, 1330, 1818, 1827, 2032, 2604
Tth111I GACNNNGTC 1 cut(s) 26
Van91I CCANNNNNTGG 1 cut(s) 945
Vha464I CTTAAG 1 cut(s) 59
VpaK11BI GGWCC 6 cut(s) 249, 791, 995, 2369, 2941, 3259
XagI CCTNNNNNAGG 1 cut(s) 3074
XapI RAATTY 9 cut(s) 144, 416, 1064, 1418, 1620, 1816, 2133, 2639, 2898
XbaI TCTAGA 1 cut(s) 2542
XceI RCATGY 3 cut(s) 953, 1319, 1409
XcmI CCANNNNNNNNNTGG 3 cut(s) 634, 2472, 2504
XmiI GTMKAC 2 cut(s) 321, 1486
XmnI GAANNNNTTC 1 cut(s) 1119
Zsp2I ATGCAT 2 cut(s) 589, 1293
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.