Rmu_co8478121.1_g000001
ERF Family

Probably involved in the RNA silencing pathway and required for the generation of small interfering RNAs (siRNAs)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8478121.1
OG0000505
Physical Location & Seq
Reverse (-)
1 .. 1414
1414 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8478121.1_g000001.1.cds

Sequence Viewer

Length: 889 bp
atgatgaagcagagttgggatgaacaagaagaggtgccgcgtctacctcagaaagtggaggtattgctccggcaaatctgtgaggacaaaaagcaaccttggccggacgctgacgccataaggcagctcgcggctcagagcgaggaggcggcaatggagaccctgaagaaaatctccaccagcaaaagcatcagaaccaccctcggccgttacatcaagtggatgatcgggcaatgtagctctgcttctccgtcgccgtccaaaacggtgcgttccgctcacgttcaaagccctggtgctacttcactcactcctcagagacttcacttcagccctccaggtgaggcagttagtgacaggtcacctgttgttgggcaattaccttattgctctggttctccatccaaagccgcggggacttgttcttatcaaagattgtctgctcatgcacaggttgatgagaggtctcgaggcgaggcatttaatcagctgtcacctgttattggtcaatcaccttattcctctggttctcctttgaaagccgcagacacttcttcttatcaaagactgtctgctcctgtacaactggatgagagatttcaaggtgatgcatttagtcattggtcatctgttggacaaccaccttattcctctgcttctccatcaaaagctgcaggcactacttcttaccaaagattgtctcctcctgtacaacttgatgatacgtttcaaggggaggcatttactgttctagagcaaccaggaaacatcaataatggacaatcaccttattattcctctggttctccattgaaagtggcacgcacttgttcgtgtcaaagaccatctgcgttggttgctcctatacaacttgataagaagtttcaag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RdRP

Protein Analysis

296

Amino Acids

32.12

Weight (kDa)

8.84

Isoelectric Point (pI)

62.67

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000505)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G19910 AT2G19910 AT2G19920 AT2G19920 AT2G19920 AT2G19920 AT2G19930 AT2G19930 AT2G19930
fragaria_vesca FvH4_1g05980 FvH4_1g05990 FvH4_1g05990 FvH4_1g09718
malus_domestica MD02G1064400.v1.1 MD15G1195300.v1.1 MD15G1195400.v1.1
prunus_persica Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1
pyrus_communis pycom02g05070 pycom15g17460
rosa_chinensis RchiOBHm_Chr2g0091671 RchiOBHm_Chr2g0091681 RchiOBHm_Chr2g0091691 RchiOBHm_Chr2g0091701 RchiOBHm_Chr2g0091711 RchiOBHm_Chr2g0091721 RchiOBHm_Chr2g0091731 RchiOBHm_Chr5g0014401
rosa_laevigata RLG00000013772 RLG00000016242 RLG00000016244 RLG00000016245 RLG00000016246 RLG00000032105
rosa_multiflora Rmu_co8374041.1_g000001 Rmu_co8478121.1_g000001 Rmu_sc0000897.1_g000005 Rmu_sc0000897.1_g000009
rosa_roxburghii Rroxscaffold_1G00062180 Rroxscaffold_1G00062190 Rroxscaffold_1G00068800 Rroxscaffold_2G00149790 Rroxscaffold_2G00149800 Rroxscaffold_2G00149810
rosa_rugosa Rorug02G0019600 Rorug02G0019700 Rorug02G0019800 Rorug02G0019900 Rorug02G0019900 Rorug02G0020000 Rorug02G0020000 Rorug02G0020100 Rorug02G0020100 Rorug02G0020200 Rorug05G0017800
rosa_samantha Rh2AG064700 Rh2AG064800 Rh2AG064900 Rh2BG063800 Rh2BG063900 Rh2BG064000 Rh2BG064100 Rh2CG065500 Rh2CG065600 Rh2CG065700 Rh2DG063900 Rh2DG064000 Rh2DG064100 Rh2DG064200 Rh2DG381700 Rh5AG056700 Rh5AG056800 Rh5AG056900 Rh5AG112600 Rh5BG109300 Rh5CG063800 Rh5CG121100 Rh5DG053100 Rh5DG108300 Rh6CG100100
rosa_wichuraiana Rw2G005560 Rw2G005580 Rw2G005590 Rw5G005370 Rw5G009770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 34
AccBSI CCGCTC 1 cut(s) 278
AccI GTMKAC 1 cut(s) 43
AccII CGCG 3 cut(s) 40, 131, 413
AciI CCGC 7 cut(s) 38, 131, 149, 276, 411, 413, 543
AcoI YGGCCR 2 cut(s) 101, 205
AcuI CTGAAG 2 cut(s) 185, 313
AcyI GRCGYC 1 cut(s) 114
AfaI GTAC 2 cut(s) 582, 711
AfiI CCNNNNNNNGG 2 cut(s) 371, 503
AgsI TTSAA 6 cut(s) 287, 538, 602, 731, 814, 887
AjnI CCWGG 3 cut(s) 292, 337, 760
AluBI AGCT 4 cut(s) 127, 240, 490, 671
AluI AGCT 4 cut(s) 127, 240, 490, 671
Alw26I GTCTC 4 cut(s) 152, 313, 471, 705
Ama87I CYCGRG 1 cut(s) 468
AoxI GGCC 2 cut(s) 101, 205
ApeKI GCWGC 2 cut(s) 124, 671
AsuHPI GGTGA 6 cut(s) 353, 354, 486, 504, 617, 777
AvaI CYCGRG 1 cut(s) 468
BanI GGYRCC 1 cut(s) 34
BarI GAAGNNNNNNTAC 2 cut(s) 229, 261
BbvI GCAGC 2 cut(s) 136, 658
BccI CCATC 3 cut(s) 409, 670, 853
BceAI ACGGC 2 cut(s) 192, 241
BciT130I CCWGG 3 cut(s) 294, 339, 762
BcoDI GTCTC 4 cut(s) 152, 313, 471, 705
BfaI CTAG 1 cut(s) 752
BfmI CTRYAG 1 cut(s) 672
BisI GCNGC 7 cut(s) 38, 125, 132, 150, 411, 543, 672
BlsI GCNGC 7 cut(s) 39, 126, 133, 151, 412, 544, 673
Bme1390I CCNGG 3 cut(s) 294, 339, 762
BmeT110I CYCGRG 1 cut(s) 468
BmiI GGNNCC 1 cut(s) 36
BmrFI CCNGG 3 cut(s) 294, 339, 762
BmsI GCATC 2 cut(s) 198, 598
BpmI CTGGAG 1 cut(s) 321
BsaHI GRCGYC 1 cut(s) 114
BsaI GGTCTC 2 cut(s) 152, 471
BsaJI CCNNGG 4 cut(s) 98, 202, 292, 411
Bsc4I CCNNNNNNNGG 2 cut(s) 371, 503
Bse1I ACTGG 1 cut(s) 591
Bse3DI GCAATG 2 cut(s) 159, 239
BseBI CCWGG 3 cut(s) 294, 339, 762
BseDI CCNNGG 4 cut(s) 98, 202, 292, 411
BseGI GGATG 4 cut(s) 25, 228, 401, 595
BseLI CCNNNNNNNGG 2 cut(s) 371, 503
BseMI GCAATG 2 cut(s) 159, 239
BseMII CTCAG 3 cut(s) 62, 149, 329
BseNI ACTGG 1 cut(s) 591
BseRI GAGGAG 3 cut(s) 158, 303, 693
BseX3I CGGCCG 1 cut(s) 205
BseXI GCAGC 2 cut(s) 136, 658
Bsh1236I CGCG 3 cut(s) 40, 131, 413
Bsh1285I CGRYCG 1 cut(s) 208
BshFI GGCC 2 cut(s) 103, 207
BshNI GGYRCC 1 cut(s) 34
BsiEI CGRYCG 1 cut(s) 208
BsiHKCI CYCGRG 1 cut(s) 468
BsiSI CCGG 2 cut(s) 70, 104
BslFI GGGAC 1 cut(s) 430
BslI CCNNNNNNNGG 2 cut(s) 371, 503
BsmAI GTCTC 4 cut(s) 152, 313, 471, 705
BsmFI GGGAC 1 cut(s) 430
BsnI GGCC 2 cut(s) 103, 207
Bso31I GGTCTC 2 cut(s) 152, 471
BsoBI CYCGRG 1 cut(s) 468
Bsp1407I TGTACA 2 cut(s) 580, 709
Bsp143I GATC 1 cut(s) 225
BspACI CCGC 7 cut(s) 38, 131, 149, 276, 411, 413, 543
BspANI GGCC 2 cut(s) 103, 207
BspCNI CTCAG 3 cut(s) 61, 148, 328
BspFNI CGCG 3 cut(s) 40, 131, 413
BspLI GGNNCC 1 cut(s) 36
BspMAI CTGCAG 1 cut(s) 676
BspT107I GGYRCC 1 cut(s) 34
BspTNI GGTCTC 2 cut(s) 152, 471
BsrBI CCGCTC 1 cut(s) 278
BsrDI GCAATG 2 cut(s) 159, 239
BsrGI TGTACA 2 cut(s) 580, 709
BsrI ACTGG 1 cut(s) 591
BssECI CCNNGG 4 cut(s) 98, 202, 292, 411
BssMI GATC 1 cut(s) 225
BssNI GRCGYC 1 cut(s) 114
BssT1I CCWWGG 1 cut(s) 98
Bst2UI CCWGG 3 cut(s) 294, 339, 762
Bst4CI ACNGT 3 cut(s) 268, 570, 748
Bst6I CTCTTC 1 cut(s) 24
BstACI GRCGYC 1 cut(s) 114
BstAUI TGTACA 2 cut(s) 580, 709
BstC8I GCNNGC 3 cut(s) 129, 676, 823
BstDEI CTNAG 3 cut(s) 48, 135, 315
BstDSI CCRYGG 1 cut(s) 411
BstEII GGTNACC 1 cut(s) 360
BstF5I GGATG 4 cut(s) 25, 228, 401, 595
BstFNI CGCG 3 cut(s) 40, 131, 413
BstKTI GATC 1 cut(s) 228
BstMAI GTCTC 4 cut(s) 152, 313, 471, 705
BstMBI GATC 1 cut(s) 225
BstMCI CGRYCG 1 cut(s) 208
BstMWI GCNNNNNNNGC 2 cut(s) 100, 857
BstNI CCWGG 3 cut(s) 294, 339, 762
BstPI GGTNACC 1 cut(s) 360
BstSCI CCNGG 3 cut(s) 292, 337, 760
BstSFI CTRYAG 1 cut(s) 672
BstUI CGCG 3 cut(s) 40, 131, 413
BstV1I GCAGC 2 cut(s) 136, 658
BstZI CGGCCG 1 cut(s) 205
BsuRI GGCC 2 cut(s) 103, 207
BtgI CCRYGG 1 cut(s) 411
BtsCI GGATG 4 cut(s) 25, 228, 401, 595
Cac8I GCNNGC 3 cut(s) 129, 676, 823
Cfr42I CCGCGG 1 cut(s) 414
CseI GACGC 3 cut(s) 29, 116, 122
Csp6I GTAC 2 cut(s) 581, 710
CviAII CATG 1 cut(s) 446
CviQI GTAC 2 cut(s) 581, 710
DdeI CTNAG 3 cut(s) 48, 135, 315
DpnI GATC 1 cut(s) 227
DpnII GATC 1 cut(s) 225
EaeI YGGCCR 2 cut(s) 101, 205
EagI CGGCCG 1 cut(s) 205
Eam1104I CTCTTC 1 cut(s) 24
EarI CTCTTC 1 cut(s) 24
EclXI CGGCCG 1 cut(s) 205
Eco130I CCWWGG 1 cut(s) 98
Eco31I GGTCTC 2 cut(s) 152, 471
Eco52I CGGCCG 1 cut(s) 205
Eco57I CTGAAG 2 cut(s) 185, 313
Eco88I CYCGRG 1 cut(s) 468
Eco91I GGTNACC 1 cut(s) 360
EcoO65I GGTNACC 1 cut(s) 360
EcoRII CCWGG 3 cut(s) 292, 337, 760
EcoT14I CCWWGG 1 cut(s) 98
EcoT22I ATGCAT 1 cut(s) 613
ErhI CCWWGG 1 cut(s) 98
FaeI CATG 1 cut(s) 449
FaiI YATR 3 cut(s) 119, 447, 866
FaqI GGGAC 1 cut(s) 430
FatI CATG 1 cut(s) 445
FauI CCCGC 1 cut(s) 406
FblI GTMKAC 1 cut(s) 43
Fnu4HI GCNGC 7 cut(s) 38, 125, 132, 150, 411, 543, 672
FokI GGATG 4 cut(s) 32, 235, 388, 602
Fsp4HI GCNGC 7 cut(s) 38, 125, 132, 150, 411, 543, 672
FspBI CTAG 1 cut(s) 752
GluI GCNGC 7 cut(s) 38, 125, 132, 150, 411, 543, 672
GsuI CTGGAG 1 cut(s) 321
HaeIII GGCC 2 cut(s) 103, 207
HapII CCGG 2 cut(s) 70, 104
HgaI GACGC 3 cut(s) 29, 116, 122
Hin1I GRCGYC 1 cut(s) 114
Hin1II CATG 1 cut(s) 449
HpaII CCGG 2 cut(s) 70, 104
HphI GGTGA 6 cut(s) 353, 354, 486, 504, 617, 777
Hpy166II GTNNAC 1 cut(s) 44
Hpy188I TCNGA 4 cut(s) 51, 138, 194, 318
Hpy188III TCNNGA 2 cut(s) 468, 752
Hpy8I GTNNAC 1 cut(s) 44
Hpy99I CGWCG 1 cut(s) 256
HpyCH4III ACNGT 3 cut(s) 268, 570, 748
HpyCH4IV ACGT 2 cut(s) 282, 725
HpyCH4V TGCA 3 cut(s) 449, 611, 674
HpyF10VI GCNNNNNNNGC 2 cut(s) 100, 857
HpyF3I CTNAG 3 cut(s) 48, 135, 315
HpySE526I ACGT 2 cut(s) 282, 725
Hsp92I GRCGYC 1 cut(s) 114
Hsp92II CATG 1 cut(s) 449
KspI CCGCGG 1 cut(s) 414
Kzo9I GATC 1 cut(s) 225
LmnI GCTCC 3 cut(s) 72, 580, 865
Lsp1109I GCAGC 2 cut(s) 136, 658
LweI GCATC 2 cut(s) 198, 598
MaeI CTAG 1 cut(s) 752
MaeII ACGT 2 cut(s) 282, 725
MaeIII GTNAC 4 cut(s) 209, 353, 360, 492
MalI GATC 1 cut(s) 227
MbiI CCGCTC 1 cut(s) 278
MboI GATC 1 cut(s) 225
MboII GAAGA 3 cut(s) 41, 178, 546
MluCI AATT 1 cut(s) 377
MmeI TCCRAC 1 cut(s) 613
Mph1103I ATGCAT 1 cut(s) 613
MseI TTAA 1 cut(s) 483
MspA1I CMGCKG 2 cut(s) 413, 490
MspI CCGG 2 cut(s) 70, 104
MspR9I CCNGG 3 cut(s) 294, 339, 762
MvaI CCWGG 3 cut(s) 294, 339, 762
MvnI CGCG 3 cut(s) 40, 131, 413
MwoI GCNNNNNNNGC 2 cut(s) 100, 857
NdeII GATC 1 cut(s) 225
NlaIII CATG 1 cut(s) 449
NlaIV GGNNCC 1 cut(s) 36
NmeAIII GCCGAG 1 cut(s) 183
NmuCI GTSAC 3 cut(s) 353, 360, 492
NsiI ATGCAT 1 cut(s) 613
PaeR7I CTCGAG 1 cut(s) 468
PkrI GCNGC 7 cut(s) 39, 126, 133, 151, 412, 544, 673
Psp6I CCWGG 3 cut(s) 292, 337, 760
PspEI GGTNACC 1 cut(s) 360
PspGI CCWGG 3 cut(s) 292, 337, 760
PspN4I GGNNCC 1 cut(s) 36
PstI CTGCAG 1 cut(s) 676
PvuII CAGCTG 1 cut(s) 490
RsaI GTAC 2 cut(s) 582, 711
RsaNI GTAC 2 cut(s) 581, 710
SacII CCGCGG 1 cut(s) 414
SaqAI TTAA 1 cut(s) 483
SatI GCNGC 7 cut(s) 38, 125, 132, 150, 411, 543, 672
Sau3AI GATC 1 cut(s) 225
ScrFI CCNGG 3 cut(s) 294, 339, 762
SfaNI GCATC 2 cut(s) 198, 598
SfcI CTRYAG 1 cut(s) 672
Sfr274I CTCGAG 1 cut(s) 468
Sfr303I CCGCGG 1 cut(s) 414
SgrBI CCGCGG 1 cut(s) 414
SlaI CTCGAG 1 cut(s) 468
SmlI CTYRAG 1 cut(s) 468
SmoI CTYRAG 1 cut(s) 468
Sse9I AATT 1 cut(s) 377
SsiI CCGC 7 cut(s) 38, 131, 149, 276, 411, 413, 543
SspMI CTAG 1 cut(s) 752
StyD4I CCNGG 3 cut(s) 292, 337, 760
StyI CCWWGG 1 cut(s) 98
TaaI ACNGT 3 cut(s) 268, 570, 748
TaiI ACGT 2 cut(s) 285, 728
TaqI TCGA 1 cut(s) 469
TasI AATT 1 cut(s) 377
TatI WGTACW 2 cut(s) 580, 709
TauI GCSGC 5 cut(s) 40, 134, 152, 413, 545
Tru1I TTAA 1 cut(s) 483
Tru9I TTAA 1 cut(s) 483
TseFI GTSAC 3 cut(s) 353, 360, 492
TseI GCWGC 2 cut(s) 124, 671
Tsp45I GTSAC 3 cut(s) 353, 360, 492
TspDTI ATGAA 2 cut(s) 20, 36
TspGWI ACGGA 1 cut(s) 240
XbaI TCTAGA 1 cut(s) 751
XhoI CTCGAG 1 cut(s) 468
XmiI GTMKAC 1 cut(s) 43
XspI CTAG 1 cut(s) 752
Zsp2I ATGCAT 1 cut(s) 613
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.