Rw2G005560
ERF Family

Probably involved in the RNA silencing pathway and required for the generation of small interfering RNAs (siRNAs)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000505
Physical Location & Seq
Reverse (-)
5227571 .. 5237813
10243 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G005560.1

Sequence Viewer

Length: 3258 bp
ATGTCCGGCGTGCAGCTACTTGACTCGGTGCAGCAATCGCTGGATCGAATATGCGACCTTAAAAACCAACCACAGCCGAACGACGGCATCAGAAGAAAGCTTGCATTGCTGACCGAGCAAGACGCCCTCTACGTGCTAAACCAAATTTACAACAGCAAAACTCCCATCAGAACCCTCAATGGATTCATCTCTTTCATGATCAAGGAACTAGAAAATGAATCTTCTTCGTCTTCTTCAAGAACAGTTTGGACCCCTCAGAGTCCCTCTGCTTCTTCTTCTTCTTCTTCTTCATTTACTAGTCCTCCACGGCCTGATCATTTTCAAGGTGAGACAATCCAGTGTGGTTCTCCTGTTAATCGACAGCCACTCTATTCTTCAGCTTCTCCTTCTCAAGCTTCCCCGACTTCTTCTTATCAGAGCCATTCCTCTCCTACTATTACTGATGCATCATTTTATTCCAGTTCTCAGCCTCAGGATGAGACTCCCAACCCATCCCCGGGGACAATGTGCAATGGACAATCTCCCTATTCCTTCCCTTCTCTCTATCAAATCCTATCTGCTCCTTTTAATACTCTTAGACAACTCTATGAGTGGTTCCGAGGTGAGACTGTCACTCACTCCCCGGTTCTTTACCATCCTGGAGACCATGGACCAGCACACTTAGAAGCTCTGGGAGAACTCGATTTCAGAAAGCAATTTCTGATTTTGAGTTATGCTGGAGGAACGAAGTTAAAAGATGTAATAGAAGCGGAGGCTATCAGGAGCTGGAAAAGTTTGCCAATGGTAAAGTTCGAGACCACAGTTTGGCATGCTTTGGGTGAAAACAATGTTGGCAAGGCTGACCGACCATTGTATTCTGATTGGGATTCTCAAAGAACACACCAGTACTATTGTTATGTTTCGGTGGATGGGAGTTACAGGTTCAAGGGTCCCTTCCTAGAGAAACCAAGAACTCTCCTGCAGAAACAGTTAGGAGATGACAATGTTTTGGAGGTGAAATTTACCCCAGGGGAATGTTCCAGTGGTACATATAGCAAGTTAATGCGAGAAGGCATTCTTGTTGGTTTGCGGCGATACGCTTTCTTTGTGTTCCGTGCTGGTAACAAAGGAAAGAATATGTGGAAAGACCCAACTTCATCACCTGTGAAATGTTACTTCGTTCGTGGGAGCTCTGATGCAGTGATAGACAGGGGTATAGGCTATAAGTTATCTGGCAAAACGATGCATGAAGGAAGATCTCTTTTCACGCATGCACACACTCGGCCTAGTTTGGCCAGCTACATGGATAGGTTATCTCTCATTTTGTCCAAGACAGTTACTTTAGAAGTAAATTGGTCACATGTCAAGGTGGAATTCATCAAAGACATAGACTGTCGCGATGAGTCTGGTAATCCCATCTATGTAGATGGAAAACCTCGCATACATACTGATGGCACAGGATTTATATCTGAGGATCTGGCTTTACTTTGTCCTGAGATAGCACGCAAAGGAGAGCAGATCAATAGTGAACACATCGAGGGACTTGATGATCCTGATGATGAACTTGAGGACAAAATTCTGGAATGGAAACAGCCAGGCACTAGAACTAGGGAACTGCCTTTGCTCATGCAGTTCCGACTTTTTTACGAAGGCCGTGCTATCAAAGGAACTTTTCTTGTCAATAAAGAGCTTCCACCAAAAACTTTGAGGATTCGATCATCAATGGTTAAAGTTGAGCCAGATCCAAAATTGTCAGATCCTCAAACAGTGAATTCACTGGAGATAGTAGGAACAAGTAAACTTAATAAGAGAACGTATTTGTCGAGGAACTTAATTGCACTTTTGTACCATGGAGGGGTGCCAAAAAAATACTTCATCGAGTTACTTATGAAAGCTTTAGAAGAGACTCGTGATGTTTTTAGCAATCCACGCGCAGCATTCAGAGTTGCCCTTAGATATGGCGAGATCGATGACAACCGTACTGTTGCGAAAATGATCTCCTGTGGTATTCCTCTTGAAGAACCATATGTGCAATATCGTCTTTCAACCCTTGTGAACATGGAAAAGATCAATCTCAAAAAAGGGAAAATTTGTATTCCTGAATCATACTATTTGATGGGGACAGCTGACCCAACAGGAAAACTTGAAAAAGATGAAGTTTGCATTATCCTAGAAAATGGACAACTACTGTCGGGAGATTTTCTAGTGTACCGGAATCCTGGTTTACACTTCGGTGATATTCATGTCCTAAAGGCCAGATATGTCGAGGAACTAGAATCTGTTGTTGGCAAGGCCAAGTACGGTATATTCTTCTCTAGTAAAGGTCCACGTTCCATTGCTGATGAAATGGCTGGTGGAGATTTTGATGGTGATCTATACTGGATCTCAAGAAACCCTCAGCTATTAAAATCGTTTAAACCAAGTGAACCCTGGATTGGAAAGTCATCTGCCCAAAAAGTTCATGGTAAAAGACCAACCGAGCTTTCAAAGGATGATCTAGAAGATGAGCTCATCAAACTATTCTTGAAGACTAGGTTTGAACCAAGCTTCGCATTGTATGAGGCAGCTCAAAACTGGATGGCTTTGATGGATAAATTTTTGACTCTAGGAGATACGAGTATTGATGTGAAAAACAAACTGAAGGATAAGATATTGCGATTGATTGATATTTACTATGAAGCTCTCGATGCACCAAAAAAAGGTTTTGAGGTTGTAGTTCCAAAAGAATTGAAGGTGCGAGAGTTCCCACATTTCATGGAGAAGAAAAACCAAGAATCCTACCATTCAAGTTCCATTTTGGGATCCATTTATGATAAGGTGGAGGAATATGAAACAGAAGACAATCTTAAAGAAATTCGGAAACTTCCTCTTTTTGAAGACGACGTTCCTGAGGACCGCTTGAAAGAATGGGAGAAACGATACAAAGAGTACCGGAAGAAGATGACCTCTGCCATGGGATATGAAGACAAAGAGAGCAAGAATCAGGCAGCCAGTATCGTCATAAGAGAGTATAAAGAGATCCTATACGAGGCTGAGGAGTTTGAGGAAAGTAAAAGGGACAGACAACAAATATATAACGACGCACTCGCTATTTATCATGTTTGTTATGACTATGCCAGGAGTGTGAGGACTGAAACTGGGGCACAAGATGTCCGGAAATGTGGCTTTGTTTGGAAAGTTGCAGGTGCAGCTCTATGCCAGCTTCATGAGGACCGGAGTTTGAAACCTGGTGAAAAGGTTTTCAGGATCTTACCCTCCGTTTTGAAGGACATTATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RdRP

Protein Analysis

1085

Amino Acids

123.53

Weight (kDa)

6.66

Isoelectric Point (pI)

41.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
4HB_RdRP3_N PF26249 6 - 69 3.7e-08 RdRP3/4/5, N-terminal four helical bundle domain
RdRP_helical PF26252 220 - 282 1.7e-07 RNA dependent RNA polymerase, helical domain
RdRP PF05183 306 - 933 3.2e-102 RNA dependent RNA polymerase
RdRP_head PF26253 964 - 1064 5.2e-12 RdRP, head domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000505)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G19910 AT2G19910 AT2G19920 AT2G19920 AT2G19920 AT2G19920 AT2G19930 AT2G19930 AT2G19930
fragaria_vesca FvH4_1g05980 FvH4_1g05990 FvH4_1g05990 FvH4_1g09718
malus_domestica MD02G1064400.v1.1 MD15G1195300.v1.1 MD15G1195400.v1.1
prunus_persica Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1
pyrus_communis pycom02g05070 pycom15g17460
rosa_chinensis RchiOBHm_Chr2g0091671 RchiOBHm_Chr2g0091681 RchiOBHm_Chr2g0091691 RchiOBHm_Chr2g0091701 RchiOBHm_Chr2g0091711 RchiOBHm_Chr2g0091721 RchiOBHm_Chr2g0091731 RchiOBHm_Chr5g0014401
rosa_laevigata RLG00000013772 RLG00000016242 RLG00000016244 RLG00000016245 RLG00000016246 RLG00000032105
rosa_multiflora Rmu_co8374041.1_g000001 Rmu_co8478121.1_g000001 Rmu_sc0000897.1_g000005 Rmu_sc0000897.1_g000009
rosa_roxburghii Rroxscaffold_1G00062180 Rroxscaffold_1G00062190 Rroxscaffold_1G00068800 Rroxscaffold_2G00149790 Rroxscaffold_2G00149800 Rroxscaffold_2G00149810
rosa_rugosa Rorug02G0019600 Rorug02G0019700 Rorug02G0019800 Rorug02G0019900 Rorug02G0019900 Rorug02G0020000 Rorug02G0020000 Rorug02G0020100 Rorug02G0020100 Rorug02G0020200 Rorug05G0017800
rosa_samantha Rh2AG064700 Rh2AG064800 Rh2AG064900 Rh2BG063800 Rh2BG063900 Rh2BG064000 Rh2BG064100 Rh2CG065500 Rh2CG065600 Rh2CG065700 Rh2DG063900 Rh2DG064000 Rh2DG064100 Rh2DG064200 Rh2DG381700 Rh5AG056700 Rh5AG056800 Rh5AG056900 Rh5AG112600 Rh5BG109300 Rh5CG063800 Rh5CG121100 Rh5DG053100 Rh5DG108300 Rh6CG100100
rosa_wichuraiana Rw2G005560 Rw2G005580 Rw2G005590 Rw5G005370 Rw5G009770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 3152
Acc36I ACCTGC 1 cut(s) 3152
AccB1I GGYRCC 1 cut(s) 1837
AccB7I CCANNNNNTGG 1 cut(s) 804
AccII CGCG 2 cut(s) 1377, 1911
AccIII TCCGGA 1 cut(s) 3132
AciI CCGC 3 cut(s) 749, 1069, 2875
AcoI YGGCCR 1 cut(s) 1272
AcsI RAATTY 8 cut(s) 144, 998, 1352, 1554, 1750, 2067, 2573, 2832
AcuI CTGAAG 2 cut(s) 360, 2639
AcyI GRCGYC 1 cut(s) 123
AfaI GTAC 7 cut(s) 887, 1027, 1826, 1960, 2189, 2279, 2909
AfiI CCNNNNNNNGG 8 cut(s) 83, 496, 497, 804, 1834, 2414, 2678, 3007
AflIII ACRYGT 1 cut(s) 1339
AhlI ACTAGT 1 cut(s) 296
AjnI CCWGG 7 cut(s) 637, 1006, 1573, 2197, 2408, 3095, 3205
AjuI GAANNNNNNNTTGG 8 cut(s) 813, 845, 2247, 2279, 2397, 2429, 2446, 2478
AleI CACNNNNGTG 1 cut(s) 2211
Alw21I GWGCWC 2 cut(s) 1172, 2490
Alw26I GTCTC 6 cut(s) 323, 473, 599, 636, 788, 1877
AlwNI CAGNNNCTG 1 cut(s) 765
Ama87I CYCGRG 1 cut(s) 496
Aor13HI TCCGGA 1 cut(s) 3132
AoxI GGCC 6 cut(s) 308, 1262, 1272, 1630, 2232, 2271
ApeKI GCWGC 6 cut(s) 13, 31, 1913, 2543, 2966, 3167
ApoI RAATTY 8 cut(s) 144, 998, 1352, 1554, 1750, 2067, 2573, 2832
Asp700I GAANNNNTTC 1 cut(s) 1053
AspLEI GCGC 1 cut(s) 1913
AspS9I GGNCC 6 cut(s) 249, 650, 929, 2303, 2872, 3190
AsuC2I CCSGG 3 cut(s) 497, 498, 623
AsuHPI GGTGA 8 cut(s) 338, 614, 830, 1006, 1131, 2225, 2360, 3221
AvaI CYCGRG 1 cut(s) 496
AvaII GGWCC 6 cut(s) 249, 650, 929, 2303, 2872, 3190
AxyI CCTNAGG 2 cut(s) 471, 2868
BaeGI GKGCMC 1 cut(s) 3124
BaeI ACNNNNGTAYC 2 cut(s) 1808, 1841
BalI TGGCCA 1 cut(s) 1274
BamHI GGATCC 1 cut(s) 2780
BanI GGYRCC 1 cut(s) 1837
BanII GRGCYC 2 cut(s) 1172, 2490
BauI CACGAG 1 cut(s) 1887
BbsI GAAGAC 5 cut(s) 222, 2513, 2823, 2862, 2949
Bbv12I GWGCWC 2 cut(s) 1172, 2490
BbvCI CCTCAGC 2 cut(s) 2376, 3012
BbvI GCAGC 6 cut(s) 25, 43, 1925, 2555, 2978, 3179
BceAI ACGGC 3 cut(s) 100, 323, 1617
BcgI CGANNNNNNTGC 2 cut(s) 1616, 1650
BciT130I CCWGG 7 cut(s) 639, 1008, 1575, 2199, 2410, 3097, 3207
BclI TGATCA 2 cut(s) 198, 313
BcnI CCSGG 3 cut(s) 497, 498, 623
BcoDI GTCTC 6 cut(s) 323, 473, 599, 636, 788, 1877
BcuI ACTAGT 1 cut(s) 296
BfmI CTRYAG 1 cut(s) 959
BfuAI ACCTGC 1 cut(s) 3152
BglII AGATCT 1 cut(s) 1235
BisI GCNGC 7 cut(s) 14, 32, 1070, 1914, 2544, 2967, 3168
BlsI GCNGC 7 cut(s) 15, 33, 1071, 1915, 2545, 2968, 3169
BmcAI AGTACT 1 cut(s) 887
Bme18I GGWCC 6 cut(s) 249, 650, 929, 2303, 2872, 3190
BmeT110I CYCGRG 1 cut(s) 496
BmgT120I GGNCC 6 cut(s) 249, 650, 929, 2303, 2872, 3190
BmiI GGNNCC 6 cut(s) 251, 596, 930, 931, 1839, 2782
BmrI ACTGGG 1 cut(s) 3126
BmsI GCATC 6 cut(s) 96, 433, 455, 1165, 1212, 2656
BmuI ACTGGG 1 cut(s) 3126
BpiI GAAGAC 5 cut(s) 222, 2513, 2823, 2862, 2949
BpmI CTGGAG 3 cut(s) 660, 738, 1778
Bpu10I CCTNAGC 2 cut(s) 2376, 3012
BpuEI CTTGAG 3 cut(s) 375, 1565, 2350
BpuMI CCSGG 3 cut(s) 497, 498, 623
Bsa29I ATCGAT 1 cut(s) 1947
BsaAI YACGTR 1 cut(s) 133
BsaBI GATNNNNATC 2 cut(s) 1444, 2349
BsaHI GRCGYC 1 cut(s) 123
BsaI GGTCTC 2 cut(s) 636, 788
BsaWI WCCGGW 4 cut(s) 2190, 2910, 3132, 3192
BsaXI ACNNNNNCTCC 2 cut(s) 286, 316
Bsc4I CCNNNNNNNGG 8 cut(s) 83, 496, 497, 804, 1834, 2414, 2678, 3007
Bse1I ACTGG 9 cut(s) 337, 459, 883, 1020, 1761, 2363, 2558, 2970, 3121
Bse21I CCTNAGG 2 cut(s) 471, 2868
Bse3DI GCAATG 3 cut(s) 104, 517, 2313
Bse8I GATNNNNATC 2 cut(s) 1444, 2349
BseAI TCCGGA 1 cut(s) 3132
BseBI CCWGG 7 cut(s) 639, 1008, 1575, 2199, 2410, 3097, 3207
BseCI ATCGAT 1 cut(s) 1947
BseGI GGATG 6 cut(s) 481, 491, 634, 913, 2476, 2562
BseJI GATNNNNATC 2 cut(s) 1444, 2349
BseLI CCNNNNNNNGG 8 cut(s) 83, 496, 497, 804, 1834, 2414, 2678, 3007
BseMI GCAATG 3 cut(s) 104, 517, 2313
BseMII CTCAG 8 cut(s) 269, 479, 485, 1440, 1464, 2390, 2859, 3003
BseNI ACTGG 9 cut(s) 337, 459, 883, 1020, 1761, 2363, 2558, 2970, 3121
BseRI GAGGAG 1 cut(s) 3029
BseSI GKGCMC 1 cut(s) 3124
BseXI GCAGC 6 cut(s) 25, 43, 1925, 2555, 2978, 3179
BsgI GTGCAG 3 cut(s) 32, 50, 3186
Bsh1236I CGCG 2 cut(s) 1377, 1911
BshFI GGCC 6 cut(s) 310, 1264, 1274, 1632, 2234, 2273
BshNI GGYRCC 1 cut(s) 1837
BshVI ATCGAT 1 cut(s) 1947
BsiHKAI GWGCWC 2 cut(s) 1172, 2490
BsiHKCI CYCGRG 1 cut(s) 496
BsiSI CCGG 7 cut(s) 6, 497, 623, 2191, 2911, 3133, 3193
BslFI GGGAC 6 cut(s) 246, 514, 915, 1533, 2113, 3050
BslI CCNNNNNNNGG 8 cut(s) 83, 496, 497, 804, 1834, 2414, 2678, 3007
BsmAI GTCTC 6 cut(s) 323, 473, 599, 636, 788, 1877
BsmFI GGGAC 6 cut(s) 246, 514, 915, 1533, 2113, 3050
BsmI GAATGC 2 cut(s) 1053, 1916
BsnI GGCC 6 cut(s) 310, 1264, 1274, 1632, 2234, 2273
Bso31I GGTCTC 2 cut(s) 636, 788
BsoBI CYCGRG 1 cut(s) 496
Bsp1286I GDGCHC 3 cut(s) 1172, 2490, 3124
Bsp13I TCCGGA 1 cut(s) 3132
Bsp19I CCATGG 3 cut(s) 646, 1828, 2931
Bsp68I TCGCGA 1 cut(s) 1377
BspACI CCGC 3 cut(s) 749, 1069, 2875
BspANI GGCC 6 cut(s) 310, 1264, 1274, 1632, 2234, 2273
BspCNI CTCAG 8 cut(s) 268, 478, 484, 1441, 1465, 2389, 2860, 3004
BspDI ATCGAT 1 cut(s) 1947
BspEI TCCGGA 1 cut(s) 3132
BspFNI CGCG 2 cut(s) 1377, 1911
BspHI TCATGA 2 cut(s) 195, 3184
BspLI GGNNCC 6 cut(s) 251, 596, 930, 931, 1839, 2782
BspMAI CTGCAG 1 cut(s) 963
BspMI ACCTGC 1 cut(s) 3152
BspT107I GGYRCC 1 cut(s) 1837
BspTNI GGTCTC 2 cut(s) 636, 788
BsrDI GCAATG 3 cut(s) 104, 517, 2313
BsrI ACTGG 9 cut(s) 337, 459, 883, 1020, 1761, 2363, 2558, 2970, 3121
BssNI GRCGYC 1 cut(s) 123
BssSI CACGAG 1 cut(s) 1887
BssT1I CCWWGG 3 cut(s) 646, 1828, 2931
Bst2BI CACGAG 1 cut(s) 1887
Bst2UI CCWGG 7 cut(s) 639, 1008, 1575, 2199, 2410, 3097, 3207
Bst6I CTCTTC 1 cut(s) 1875
BstACI GRCGYC 1 cut(s) 123
BstBAI YACGTR 1 cut(s) 133
BstC8I GCNNGC 7 cut(s) 11, 102, 810, 1251, 1276, 1483, 3179
BstDSI CCRYGG 4 cut(s) 305, 646, 1828, 2931
BstENI CCTNNNNNAGG 1 cut(s) 3005
BstF5I GGATG 6 cut(s) 481, 491, 634, 913, 2476, 2562
BstFNI CGCG 2 cut(s) 1377, 1911
BstHHI GCGC 1 cut(s) 1913
BstMAI GTCTC 6 cut(s) 323, 473, 599, 636, 788, 1877
BstMWI GCNNNNNNNGC 6 cut(s) 37, 106, 115, 1908, 2666, 3167
BstNI CCWGG 7 cut(s) 639, 1008, 1575, 2199, 2410, 3097, 3207
BstNSI RCATGY 3 cut(s) 812, 1253, 1343
BstSFI CTRYAG 1 cut(s) 959
BstSLI GKGCMC 1 cut(s) 3124
BstUI CGCG 2 cut(s) 1377, 1911
BstV1I GCAGC 6 cut(s) 25, 43, 1925, 2555, 2978, 3179
BstV2I GAAGAC 5 cut(s) 222, 2513, 2823, 2862, 2949
BstX2I RGATCY 8 cut(s) 1235, 1453, 1720, 1735, 2361, 2780, 2997, 3225
BstXI CCANNNNNNTGG 1 cut(s) 1282
BstYI RGATCY 8 cut(s) 1235, 1453, 1720, 1735, 2361, 2780, 2997, 3225
Bsu15I ATCGAT 1 cut(s) 1947
Bsu36I CCTNAGG 2 cut(s) 471, 2868
BsuRI GGCC 6 cut(s) 310, 1264, 1274, 1632, 2234, 2273
BsuTUI ATCGAT 1 cut(s) 1947
BtgI CCRYGG 4 cut(s) 305, 646, 1828, 2931
BtgZI GCGATG 1 cut(s) 1392
BtsCI GGATG 6 cut(s) 481, 491, 634, 913, 2476, 2562
BtsI GCAGTG 1 cut(s) 1185
BtsIMutI CAGTG 5 cut(s) 344, 1027, 1185, 1752, 1754
BtuMI TCGCGA 1 cut(s) 1377
BveI ACCTGC 1 cut(s) 3152
Cac8I GCNNGC 7 cut(s) 11, 102, 810, 1251, 1276, 1483, 3179
CaiI CAGNNNCTG 1 cut(s) 765
CciI TCATGA 2 cut(s) 195, 3184
CfoI GCGC 1 cut(s) 1913
Cfr13I GGNCC 6 cut(s) 249, 650, 929, 2303, 2872, 3190
Cfr9I CCCGGG 1 cut(s) 496
ClaI ATCGAT 1 cut(s) 1947
CseI GACGC 2 cut(s) 131, 3068
CsiI ACCWGGT 1 cut(s) 3205
Csp6I GTAC 7 cut(s) 886, 1026, 1825, 1959, 2188, 2278, 2908
CviQI GTAC 7 cut(s) 886, 1026, 1825, 1959, 2188, 2278, 2908
DraI TTTAAA 1 cut(s) 2395
EaeI YGGCCR 1 cut(s) 1272
Eam1104I CTCTTC 1 cut(s) 1875
EarI CTCTTC 1 cut(s) 1875
Ecl136II GAGCTC 2 cut(s) 1170, 2488
Eco130I CCWWGG 3 cut(s) 646, 1828, 2931
Eco24I GRGCYC 2 cut(s) 1172, 2490
Eco31I GGTCTC 2 cut(s) 636, 788
Eco47I GGWCC 6 cut(s) 249, 650, 929, 2303, 2872, 3190
Eco53kI GAGCTC 2 cut(s) 1170, 2488
Eco57I CTGAAG 2 cut(s) 360, 2639
Eco81I CCTNAGG 2 cut(s) 471, 2868
Eco88I CYCGRG 1 cut(s) 496
EcoICRI GAGCTC 2 cut(s) 1170, 2488
EcoNI CCTNNNNNAGG 1 cut(s) 3005
EcoO109I RGGNCCY 1 cut(s) 929
EcoRI GAATTC 2 cut(s) 1352, 1750
EcoRII CCWGG 7 cut(s) 637, 1006, 1573, 2197, 2408, 3095, 3205
EcoT14I CCWWGG 3 cut(s) 646, 1828, 2931
EcoT22I ATGCAT 2 cut(s) 448, 1227
EcoT38I GRGCYC 2 cut(s) 1172, 2490
ErhI CCWWGG 3 cut(s) 646, 1828, 2931
FalI AAGNNNNNCTT 6 cut(s) 917, 949, 1041, 1073, 2808, 2840
FaqI GGGAC 6 cut(s) 246, 514, 915, 1533, 2113, 3050
FauNDI CATATG 1 cut(s) 2005
FbaI TGATCA 2 cut(s) 198, 313
Fnu4HI GCNGC 7 cut(s) 14, 32, 1070, 1914, 2544, 2967, 3168
FokI GGATG 6 cut(s) 478, 488, 621, 920, 2483, 2569
FriOI GRGCYC 2 cut(s) 1172, 2490
Fsp4HI GCNGC 7 cut(s) 14, 32, 1070, 1914, 2544, 2967, 3168
GlaI GCGC 1 cut(s) 1912
GluI GCNGC 7 cut(s) 14, 32, 1070, 1914, 2544, 2967, 3168
GsuI CTGGAG 3 cut(s) 660, 738, 1778
HaeIII GGCC 6 cut(s) 310, 1264, 1274, 1632, 2234, 2273
HapII CCGG 7 cut(s) 6, 497, 623, 2191, 2911, 3133, 3193
HgaI GACGC 2 cut(s) 131, 3068
HhaI GCGC 1 cut(s) 1913
Hin1I GRCGYC 1 cut(s) 123
Hin6I GCGC 1 cut(s) 1911
HinP1I GCGC 1 cut(s) 1911
HindIII AAGCTT 4 cut(s) 98, 393, 1872, 2524
HpaII CCGG 7 cut(s) 6, 497, 623, 2191, 2911, 3133, 3193
HphI GGTGA 8 cut(s) 338, 614, 830, 1006, 1131, 2225, 2360, 3221
Hpy166II GTNNAC 7 cut(s) 1508, 1778, 2035, 2188, 2204, 2306, 2405
Hpy8I GTNNAC 7 cut(s) 1508, 1778, 2035, 2188, 2204, 2306, 2405
Hpy99I CGWCG 3 cut(s) 86, 2864, 3062
HpyCH4IV ACGT 4 cut(s) 132, 1793, 2308, 2862
HpyF10VI GCNNNNNNNGC 6 cut(s) 37, 106, 115, 1908, 2666, 3167
HpySE526I ACGT 4 cut(s) 132, 1793, 2308, 2862
Hsp92I GRCGYC 1 cut(s) 123
HspAI GCGC 1 cut(s) 1911
KflI GGGWCCC 1 cut(s) 929
Kpn2I TCCGGA 1 cut(s) 3132
Ksp22I TGATCA 2 cut(s) 198, 313
LmnI GCTCC 3 cut(s) 565, 762, 1167
Lsp1109I GCAGC 6 cut(s) 25, 43, 1925, 2555, 2978, 3179
LweI GCATC 6 cut(s) 96, 433, 455, 1165, 1212, 2656
MabI ACCWGGT 1 cut(s) 3205
MaeII ACGT 4 cut(s) 132, 1793, 2308, 2862
MaeIII GTNAC 7 cut(s) 610, 914, 1100, 1151, 1315, 1335, 1860
MflI RGATCY 8 cut(s) 1235, 1453, 1720, 1735, 2361, 2780, 2997, 3225
MhlI GDGCHC 3 cut(s) 1172, 2490, 3124
MlsI TGGCCA 1 cut(s) 1274
MluNI TGGCCA 1 cut(s) 1274
MlyI GAGTC 6 cut(s) 17, 268, 475, 1391, 1879, 2575
MmeI TCCRAC 1 cut(s) 1639
Mox20I TGGCCA 1 cut(s) 1274
Mph1103I ATGCAT 2 cut(s) 448, 1227
MroI TCCGGA 1 cut(s) 3132
MroXI GAANNNNTTC 1 cut(s) 1053
MscI TGGCCA 1 cut(s) 1274
MslI CAYNNNNRTG 2 cut(s) 1428, 2211
Msp20I TGGCCA 1 cut(s) 1274
MspA1I CMGCKG 1 cut(s) 2105
MspI CCGG 7 cut(s) 6, 497, 623, 2191, 2911, 3133, 3193
MssI GTTTAAAC 1 cut(s) 2395
Mva1269I GAATGC 2 cut(s) 1053, 1916
MvaI CCWGG 7 cut(s) 639, 1008, 1575, 2199, 2410, 3097, 3207
MvnI CGCG 2 cut(s) 1377, 1911
MwoI GCNNNNNNNGC 6 cut(s) 37, 106, 115, 1908, 2666, 3167
NciI CCSGG 3 cut(s) 497, 498, 623
NcoI CCATGG 3 cut(s) 646, 1828, 2931
NdeI CATATG 1 cut(s) 2005
NlaIV GGNNCC 6 cut(s) 251, 596, 930, 931, 1839, 2782
NmeAIII GCCGAG 1 cut(s) 1240
NmuCI GTSAC 2 cut(s) 610, 1335
NruI TCGCGA 1 cut(s) 1377
NsiI ATGCAT 2 cut(s) 448, 1227
NspI RCATGY 3 cut(s) 812, 1253, 1343
OliI CACNNNNGTG 1 cut(s) 2211
PaeI GCATGC 2 cut(s) 812, 1253
PagI TCATGA 2 cut(s) 195, 3184
PaqCI CACCTGC 1 cut(s) 3152
PasI CCCWGGG 1 cut(s) 1007
PciI ACATGT 1 cut(s) 1339
PcsI WCGNNNNNNNCGW 2 cut(s) 129, 1799
PctI GAATGC 2 cut(s) 1053, 1916
PdmI GAANNNNTTC 1 cut(s) 1053
PfeI GAWTC 9 cut(s) 183, 218, 866, 1690, 2081, 2194, 2255, 2753, 2959
PflMI CCANNNNNTGG 1 cut(s) 804
PfoI TCCNGGA 1 cut(s) 637
PkrI GCNGC 7 cut(s) 15, 33, 1071, 1915, 2545, 2968, 3169
PleI GAGTC 6 cut(s) 17, 267, 475, 1390, 1879, 2575
PmeI GTTTAAAC 1 cut(s) 2395
PpsI GAGTC 6 cut(s) 17, 267, 475, 1390, 1879, 2575
Ppu21I YACGTR 1 cut(s) 133
PpuMI RGGWCCY 1 cut(s) 929
PscI ACATGT 1 cut(s) 1339
Psp124BI GAGCTC 2 cut(s) 1172, 2490
Psp5II RGGWCCY 1 cut(s) 929
Psp6I CCWGG 7 cut(s) 637, 1006, 1573, 2197, 2408, 3095, 3205
PspGI CCWGG 7 cut(s) 637, 1006, 1573, 2197, 2408, 3095, 3205
PspN4I GGNNCC 6 cut(s) 251, 596, 930, 931, 1839, 2782
PspPI GGNCC 6 cut(s) 249, 650, 929, 2303, 2872, 3190
PspPPI RGGWCCY 1 cut(s) 929
PstI CTGCAG 1 cut(s) 963
PstNI CAGNNNCTG 1 cut(s) 765
PsuI RGATCY 8 cut(s) 1235, 1453, 1720, 1735, 2361, 2780, 2997, 3225
PvuII CAGCTG 1 cut(s) 2105
RruI TCGCGA 1 cut(s) 1377
RsaI GTAC 7 cut(s) 887, 1027, 1826, 1960, 2189, 2279, 2909
RsaNI GTAC 7 cut(s) 886, 1026, 1825, 1959, 2188, 2278, 2908
RseI CAYNNNNRTG 2 cut(s) 1428, 2211
SacI GAGCTC 2 cut(s) 1172, 2490
SatI GCNGC 7 cut(s) 14, 32, 1070, 1914, 2544, 2967, 3168
Sau96I GGNCC 6 cut(s) 249, 650, 929, 2303, 2872, 3190
ScaI AGTACT 1 cut(s) 887
SchI GAGTC 6 cut(s) 17, 268, 475, 1391, 1879, 2575
SduI GDGCHC 3 cut(s) 1172, 2490, 3124
SexAI ACCWGGT 1 cut(s) 3205
SfaNI GCATC 6 cut(s) 96, 433, 455, 1165, 1212, 2656
SfcI CTRYAG 1 cut(s) 959
SinI GGWCC 6 cut(s) 249, 650, 929, 2303, 2872, 3190
SmaI CCCGGG 1 cut(s) 498
SmiMI CAYNNNNRTG 2 cut(s) 1428, 2211
SmlI CTYRAG 3 cut(s) 390, 1544, 2365
SmoI CTYRAG 3 cut(s) 390, 1544, 2365
SpeI ACTAGT 1 cut(s) 296
SphI GCATGC 2 cut(s) 812, 1253
SsiI CCGC 3 cut(s) 749, 1069, 2875
SstI GAGCTC 2 cut(s) 1172, 2490
StyI CCWWGG 3 cut(s) 646, 1828, 2931
TaiI ACGT 4 cut(s) 135, 1796, 2311, 2865
TaqII GACCGA 2 cut(s) 128, 858
TatI WGTACW 1 cut(s) 885
TauI GCSGC 1 cut(s) 1072
TfiI GAWTC 9 cut(s) 183, 218, 866, 1690, 2081, 2194, 2255, 2753, 2959
TscAI CASTG 5 cut(s) 344, 1027, 1185, 1752, 1761
TseFI GTSAC 2 cut(s) 610, 1335
TseI GCWGC 6 cut(s) 13, 31, 1913, 2543, 2966, 3167
Tsp45I GTSAC 2 cut(s) 610, 1335
TspGWI ACGGA 2 cut(s) 1082, 3226
TspMI CCCGGG 1 cut(s) 496
TspRI CASTG 5 cut(s) 344, 1027, 1185, 1752, 1761
Van91I CCANNNNNTGG 1 cut(s) 804
VpaK11BI GGWCC 6 cut(s) 249, 650, 929, 2303, 2872, 3190
XagI CCTNNNNNAGG 1 cut(s) 3005
XapI RAATTY 8 cut(s) 144, 998, 1352, 1554, 1750, 2067, 2573, 2832
XbaI TCTAGA 1 cut(s) 2476
XceI RCATGY 3 cut(s) 812, 1253, 1343
XcmI CCANNNNNNNNNTGG 2 cut(s) 2406, 2438
XmaI CCCGGG 1 cut(s) 496
XmnI GAANNNNTTC 1 cut(s) 1053
ZrmI AGTACT 1 cut(s) 887
Zsp2I ATGCAT 2 cut(s) 448, 1227
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.