RLG00000032105
ERF Family

Probably involved in the RNA silencing pathway and required for the generation of small interfering RNAs (siRNAs)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0000505
Physical Location & Seq
Reverse (-)
10970137 .. 10984912
14776 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000032105

Sequence Viewer

Length: 3138 bp
ATGTTCGGCGTGCAGCTACTCGACTCCGTGCAGCAATCGCTGGATCGAATATGTCACCTTAAGAAGCAACCGCAGCCGGATGACGGTATCAGAAGAAAGCTTGCATTGCTGAGCGAGCAAGACGCCCTCTACGTGCTGGACCAAATTTACAACAGCAAAACTGAGATCAGAACCCTCAATGGATTCATTGCTTTCATGATCAGGAAACTAGAGAATGAATCTTCTTCGTCTTCTCCTTCCAGAACAGTCTGGACCCCTCAGAGTCCCTCTGCTTCTTCTTCTTCTGTTACTAGTCCTGCACAGCTTTATCACAGCCCTGATCATTCTCAAGTTGTAGACCAGTCTGAAGAGAATGGACTCTGTTCCTCTGTTTTTCAGCGGCTATATTTGAGCTCTCAAGGTGAGACAATTCCTTGTGGTTCTCCTGGTGTAGAGCAACATCAAGACTATAATCGACAGCCACTCTATTCTTCAGCTTCTCCTTCTGAAGCTTTCCAGACTTCTTACTATCAGAGCCATTCTTCTCCTACTAGTACTGATGCACCATTTTATTCCAGTTCTCAGCCTCAGGGTGTCCATGTTTCTCCTTTTCAAACCCTATCTGCCTCTCCAGTTACTCCTGTTCAACTTTATAAGAGCTTTCAAGGTGAGACTGTCACTCACCCCCCAGTTCTATACCATCCTGGGGACCATGGACCAGCACACTTAGAAGCTCTGGGAGAACTCGAGTTCAGAAGACAATTTCTAATATTAAGTTATGCTGGAGGAACGAAGTTAAAAGATGTAATAGAAGTGGAGGCTATTAGGCGCTGGAAAAATTTGCCAATGGTAAACTTTGAGACCAAAGTTTGGCATGCTTTGGGTGAAAACAATGCTGGCAAGGCTGACCGACAATGGTGTTCTGATTGGGATTCTCAAAGAACACACCACTACTATTGTTATGTTTCCGTGGATGGGAGTTACAAGTTCAAGGGTCCCTTCCCAGACAAACCAAGAACTCTCCTACAGAAATGGTTAGAAGATGACAATGTTTTAGAGGTGAAATTTACCCAAGGGGAAGGTTCCAGTATTCCTTTCGACAATAATTATGGTACATATAGCAAGTTAATGCGAGAAGGCATTCTTGTTGGTTTGCGGCGATATGGTCACTTTGTGTACAGTGTTGGTGGTAACAAAGGAAAGAATAAGTTGAAAGACCCAACTTCATCACCTGTGAAATGTTACTTTGTTCGTATGAGCTCTGATGCAGCGATAGACATGGGTAAATTCTATAAGTTAGCTGGCAAAACGATGCATGAAGGAAGATCTCTTTTGACGCACGTACACACTGGGCCTAGTTTGGCTAATTACATGGCTAGGTATAGCAAATTTCTTTCCCGAGCTACAGTTACTTTAGAAGTGAATTGGTCACTTATCAAGGTTGAGTTCATCAATGAAGAACAATGTCTTGATGAGTCTGGTAATCCCATCTATGTAGATGGAAAAGCTCGTATACATACTGATGGCACGGGATTTATATCTGAGGATCTCGCTTTACTTTGTCCTGAGATAGCACACAAAGGAGAGCTTATCAATAATGAACACATCGAGGGACTTGATGATCCTGATGATGAACTTGAGGACAACATTCTGGAATGGAAACAGCCAGGCACTAGAACTCAGGAACTGCCTTTGCTCATGCAGTTCCGACTTTTTTACAAAGGCTATGCTATCAAAGGAACTTTTCTTGTCAATAAACTGCTTCCACCAAACACATTGCAGATTCGACCATCAATGGTCAAAGTTGAGCCAGATCCAAAATTGTCAGATCCTCAAACAGTGAATTCACTGGAGATAGTAGGAACAAGTAAACTTACTAAGAGAACAACTTTATCGAGGAATTTGATTGCACTTTTATACCATGGAGGGGTGCCAAAAGAATACTTCATCGAGTTACTTGTGAAAGCTTTAGAAGAGACTCGTAATGTTTATAGCAATAAACGTGCAGCATTCAGAGTTGCCCTTAGATATGGCGAGATCGACGACAATAGTAATGTTGTGAAAATAATCTCTTGTGGTATTCCTCTTGAAGAACCATATATGCAATATCGTCTTTCAGCCCTTATGAACATGGAAAGATCAATCTTAAAAAAGGGAAAATTTGTTCTAGTGTACCGGAATCCTGGTGTACACTTCGGTGATATTCATGTCCTAAAAGCCACGTATTACGGTATATTCTTCTCTTGTAAAGGTCCACGTTCCGTTGCGGATGAAATGGGTGGTGGAGATTTTGATGGTCATCTATACTGGATCTCAAGAAACCCTCAGTCATCTACCCAAAAAGTTCATGGTAAAAGACCAACCGAGCTTTCAAATGTTGATCTAGAAGATGAGCTCATCAAACTATTCTTGAAGACTAGGTTTGAACCAAGTGAGGCAGCTCAAAACTGGATGGCATGCATGGATAAATTTTTGAGTTTAGGAGATACGAGTATTGATGAGAAAAACGAACTGAAGGCAAAGATACTACAATTGATCGATATTTACTATGAAGCCCTTGATGCTCCCAAACAAGGTTTTAAGGTTGTAGTTCCAAAAGAATTGAAGGTGAAAAAGTTCCCACATTTCATGGAGAAGAAACCCCTAGATTCCTACCATTCAATTTCCATTTTGGGATCAATTTATGATAAGGTGGATGAATATGAAACAGAAGACAGATCTCTCAAAGAAATTCAGAAACTTCCACTTTTTGAAGTTGACGTTCCTGAGGGTAGCTTGAAAGAATGGAAGAAACTATACCAAGATTACCGGAATGAGATGACCTCTGCCATGGGAGCCGAAGATAAACAGGGCCAGAATCAGGCAGCCAATATCGTCATAAGAAAGTATAAAGAGATCCTATACAAGGCTGAGGAGTTTGAGCAAAGTGATATGCGGCACAGACAACAAATATATAATGACGCACTCGCTATTTATCACGTTTGTTATGACTATGCCAAGAGTGTTAGGACTGAAACTGGTGCACAAGATGTCAAGAAATGTGGCTTTGCTTGGAAAGTTGCAGGTGCAGCTCTATGCCAGCTTCACGAAGACTGGAGTTTGAAACCTGGTGAAAAGGTTTTCAGAGTCTTACCCTCTGTTTTGAAGGACATTATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RdRP

Protein Analysis

1046

Amino Acids

118.67

Weight (kDa)

6.47

Isoelectric Point (pI)

47.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
4HB_RdRP3_N PF26249 6 - 69 2.4e-07 RdRP3/4/5, N-terminal four helical bundle domain
RdRP_helical PF26252 235 - 297 2e-06 RNA dependent RNA polymerase, helical domain
RdRP PF05183 322 - 710 8.8e-38 RNA dependent RNA polymerase
RdRP PF05183 707 - 891 3.1e-32 RNA dependent RNA polymerase
RdRP_head PF26253 919 - 1023 3.5e-09 RdRP, head domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000505)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G19910 AT2G19910 AT2G19920 AT2G19920 AT2G19920 AT2G19920 AT2G19930 AT2G19930 AT2G19930
fragaria_vesca FvH4_1g05980 FvH4_1g05990 FvH4_1g05990 FvH4_1g09718
malus_domestica MD02G1064400.v1.1 MD15G1195300.v1.1 MD15G1195400.v1.1
prunus_persica Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1
pyrus_communis pycom02g05070 pycom15g17460
rosa_chinensis RchiOBHm_Chr2g0091671 RchiOBHm_Chr2g0091681 RchiOBHm_Chr2g0091691 RchiOBHm_Chr2g0091701 RchiOBHm_Chr2g0091711 RchiOBHm_Chr2g0091721 RchiOBHm_Chr2g0091731 RchiOBHm_Chr5g0014401
rosa_laevigata RLG00000013772 RLG00000016242 RLG00000016244 RLG00000016245 RLG00000016246 RLG00000032105
rosa_multiflora Rmu_co8374041.1_g000001 Rmu_co8478121.1_g000001 Rmu_sc0000897.1_g000005 Rmu_sc0000897.1_g000009
rosa_roxburghii Rroxscaffold_1G00062180 Rroxscaffold_1G00062190 Rroxscaffold_1G00068800 Rroxscaffold_2G00149790 Rroxscaffold_2G00149800 Rroxscaffold_2G00149810
rosa_rugosa Rorug02G0019600 Rorug02G0019700 Rorug02G0019800 Rorug02G0019900 Rorug02G0019900 Rorug02G0020000 Rorug02G0020000 Rorug02G0020100 Rorug02G0020100 Rorug02G0020200 Rorug05G0017800
rosa_samantha Rh2AG064700 Rh2AG064800 Rh2AG064900 Rh2BG063800 Rh2BG063900 Rh2BG064000 Rh2BG064100 Rh2CG065500 Rh2CG065600 Rh2CG065700 Rh2DG063900 Rh2DG064000 Rh2DG064100 Rh2DG064200 Rh2DG381700 Rh5AG056700 Rh5AG056800 Rh5AG056900 Rh5AG112600 Rh5BG109300 Rh5CG063800 Rh5CG121100 Rh5DG053100 Rh5DG108300 Rh6CG100100
rosa_wichuraiana Rw2G005560 Rw2G005580 Rw2G005590 Rw5G005370 Rw5G009770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 633
AarI CACCTGC 1 cut(s) 3032
Acc36I ACCTGC 1 cut(s) 3032
AccB1I GGYRCC 1 cut(s) 1909
AccB7I CCANNNNNTGG 1 cut(s) 849
AccI GTMKAC 2 cut(s) 336, 1492
AciI CCGC 5 cut(s) 71, 379, 1135, 2246, 2914
AclWI GGATC 8 cut(s) 51, 1533, 1595, 1787, 1802, 2297, 2662, 2869
AcuI CTGAAG 4 cut(s) 366, 456, 507, 2513
AcyI GRCGYC 1 cut(s) 123
AdeI CACNNNGTG 1 cut(s) 1153
AfaI GTAC 6 cut(s) 535, 1093, 1157, 1323, 2153, 2169
AfiI CCNNNNNNNGG 8 cut(s) 83, 685, 849, 954, 1906, 2552, 2839, 2884
AflII CTTAAG 1 cut(s) 59
AhlI ACTAGT 2 cut(s) 290, 530
AjnI CCWGG 5 cut(s) 424, 682, 1645, 2161, 3085
AjuI GAANNNNNNNTTGG 2 cut(s) 2332, 2364
AleI CACNNNNGTG 1 cut(s) 2175
AloI GAACNNNNNNTCC 2 cut(s) 2127, 2159
Alw21I GWGCWC 4 cut(s) 395, 1241, 2376, 3004
Alw26I GTCTC 4 cut(s) 398, 644, 833, 1949
Alw44I GTGCAC 1 cut(s) 3000
AlwI GGATC 8 cut(s) 51, 1533, 1595, 1787, 1802, 2297, 2662, 2869
AlwNI CAGNNNCTG 1 cut(s) 1666
Ama87I CYCGRG 2 cut(s) 725, 1377
AoxI GGCC 2 cut(s) 1331, 2830
ApaLI GTGCAC 1 cut(s) 3000
ApeKI GCWGC 8 cut(s) 13, 31, 73, 1247, 1985, 2417, 2843, 3047
Asp700I GAANNNNTTC 2 cut(s) 1119, 2594
AspLEI GCGC 1 cut(s) 810
AspS9I GGNCC 8 cut(s) 139, 252, 688, 695, 974, 1331, 2231, 2830
AvaI CYCGRG 2 cut(s) 725, 1377
AvaII GGWCC 6 cut(s) 139, 252, 688, 695, 974, 2231
AxyI CCTNAGG 2 cut(s) 567, 2745
BaeGI GKGCMC 1 cut(s) 3004
BanI GGYRCC 1 cut(s) 1909
BanII GRGCYC 3 cut(s) 395, 1241, 2376
BarI GAAGNNNNNNTAC 2 cut(s) 2759, 2791
BbsI GAAGAC 5 cut(s) 222, 742, 2399, 2697, 3075
Bbv12I GWGCWC 4 cut(s) 395, 1241, 2376, 3004
BbvCI CCTCAGC 1 cut(s) 2889
BbvI GCAGC 8 cut(s) 25, 43, 85, 1259, 1997, 2429, 2855, 3059
BccI CCATC 8 cut(s) 687, 947, 1472, 1475, 1496, 1777, 2267, 2425
BciT130I CCWGG 5 cut(s) 426, 684, 1647, 2163, 3087
BclI TGATCA 2 cut(s) 198, 319
BcoDI GTCTC 4 cut(s) 398, 644, 833, 1949
BcuI ACTAGT 2 cut(s) 290, 530
BfmI CTRYAG 2 cut(s) 1004, 1383
BfoI RGCGCY 1 cut(s) 811
BfrI CTTAAG 1 cut(s) 59
BfuAI ACCTGC 1 cut(s) 3032
BglII AGATCT 2 cut(s) 1304, 2696
BlpI GCTNAGC 1 cut(s) 110
BmcAI AGTACT 1 cut(s) 535
Bme1390I CCNGG 5 cut(s) 426, 684, 1647, 2163, 3087
Bme18I GGWCC 6 cut(s) 139, 252, 688, 695, 974, 2231
BmeT110I CYCGRG 2 cut(s) 725, 1377
BmgT120I GGNCC 8 cut(s) 139, 252, 688, 695, 974, 1331, 2231, 2830
BmiI GGNNCC 7 cut(s) 254, 689, 975, 976, 1063, 1911, 2815
BmrFI CCNGG 5 cut(s) 426, 684, 1647, 2163, 3087
BmrI ACTGGG 2 cut(s) 662, 1338
BmsI GCATC 4 cut(s) 529, 1234, 1281, 2530
BmuI ACTGGG 2 cut(s) 662, 1338
BpiI GAAGAC 5 cut(s) 222, 742, 2399, 2697, 3075
BplI GAGNNNNNCTC 2 cut(s) 2786, 2818
BpmI CTGGAG 4 cut(s) 594, 783, 1850, 3094
Bpu10I CCTNAGC 1 cut(s) 2889
Bpu1102I GCTNAGC 1 cut(s) 110
BpuEI CTTGAG 4 cut(s) 312, 381, 1637, 2278
Bsa29I ATCGAT 1 cut(s) 2517
BsaAI YACGTR 3 cut(s) 133, 1321, 2202
BsaBI GATNNNNATC 2 cut(s) 1516, 2277
BsaHI GRCGYC 1 cut(s) 123
BsaI GGTCTC 1 cut(s) 833
BsaJI CCNNGG 6 cut(s) 683, 691, 948, 1051, 1900, 2808
BsaWI WCCGGW 2 cut(s) 2154, 2787
Bsc4I CCNNNNNNNGG 8 cut(s) 83, 685, 849, 954, 1906, 2552, 2839, 2884
Bse21I CCTNAGG 2 cut(s) 567, 2745
Bse3DI GCAATG 3 cut(s) 104, 186, 1754
Bse8I GATNNNNATC 2 cut(s) 1516, 2277
BseBI CCWGG 5 cut(s) 426, 684, 1647, 2163, 3087
BseCI ATCGAT 1 cut(s) 2517
BseDI CCNNGG 6 cut(s) 683, 691, 948, 1051, 1900, 2808
BseGI GGATG 6 cut(s) 85, 679, 958, 2254, 2436, 2680
BseJI GATNNNNATC 2 cut(s) 1516, 2277
BseLI CCNNNNNNNGG 8 cut(s) 83, 685, 849, 954, 1906, 2552, 2839, 2884
BseMI GCAATG 3 cut(s) 104, 186, 1754
BseRI GAGGAG 1 cut(s) 2906
BseSI GKGCMC 1 cut(s) 3004
BseXI GCAGC 8 cut(s) 25, 43, 85, 1259, 1997, 2429, 2855, 3059
BsgI GTGCAG 5 cut(s) 32, 50, 282, 2004, 3066
BshFI GGCC 2 cut(s) 1333, 2832
BshNI GGYRCC 1 cut(s) 1909
BshVI ATCGAT 1 cut(s) 2517
BsiHKAI GWGCWC 4 cut(s) 395, 1241, 2376, 3004
BsiHKCI CYCGRG 2 cut(s) 725, 1377
BsiSI CCGG 3 cut(s) 77, 2155, 2788
BslFI GGGAC 4 cut(s) 249, 701, 960, 1605
BslI CCNNNNNNNGG 8 cut(s) 83, 685, 849, 954, 1906, 2552, 2839, 2884
BsmAI GTCTC 4 cut(s) 398, 644, 833, 1949
BsmFI GGGAC 4 cut(s) 249, 701, 960, 1605
BsmI GAATGC 2 cut(s) 1119, 1988
BsnI GGCC 2 cut(s) 1333, 2832
Bso31I GGTCTC 1 cut(s) 833
BsoBI CYCGRG 2 cut(s) 725, 1377
Bsp1286I GDGCHC 4 cut(s) 395, 1241, 2376, 3004
Bsp1407I TGTACA 2 cut(s) 1155, 2167
Bsp1720I GCTNAGC 1 cut(s) 110
Bsp19I CCATGG 3 cut(s) 691, 1900, 2808
BspACI CCGC 5 cut(s) 71, 379, 1135, 2246, 2914
BspANI GGCC 2 cut(s) 1333, 2832
BspDI ATCGAT 1 cut(s) 2517
BspHI TCATGA 1 cut(s) 195
BspLI GGNNCC 7 cut(s) 254, 689, 975, 976, 1063, 1911, 2815
BspMI ACCTGC 1 cut(s) 3032
BspPI GGATC 8 cut(s) 51, 1533, 1595, 1787, 1802, 2297, 2662, 2869
BspT107I GGYRCC 1 cut(s) 1909
BspTI CTTAAG 1 cut(s) 59
BspTNI GGTCTC 1 cut(s) 833
BsrDI GCAATG 3 cut(s) 104, 186, 1754
BsrGI TGTACA 2 cut(s) 1155, 2167
BssECI CCNNGG 6 cut(s) 683, 691, 948, 1051, 1900, 2808
BssNAI GTATAC 1 cut(s) 1493
BssNI GRCGYC 1 cut(s) 123
BssT1I CCWWGG 4 cut(s) 691, 1051, 1900, 2808
Bst1107I GTATAC 1 cut(s) 1493
Bst2UI CCWGG 5 cut(s) 426, 684, 1647, 2163, 3087
Bst4CI ACNGT 7 cut(s) 86, 247, 655, 1160, 1387, 1819, 2210
Bst6I CTCTTC 2 cut(s) 342, 1947
BstACI GRCGYC 1 cut(s) 123
BstAFI CTTAAG 1 cut(s) 59
BstAUI TGTACA 2 cut(s) 1155, 2167
BstBAI YACGTR 3 cut(s) 133, 1321, 2202
BstC8I GCNNGC 8 cut(s) 11, 102, 116, 855, 877, 1282, 2437, 3059
BstDSI CCRYGG 4 cut(s) 691, 948, 1900, 2808
BstENI CCTNNNNNAGG 1 cut(s) 2882
BstF5I GGATG 6 cut(s) 85, 679, 958, 2254, 2436, 2680
BstH2I RGCGCY 1 cut(s) 811
BstHHI GCGC 1 cut(s) 810
BstMAI GTCTC 4 cut(s) 398, 644, 833, 1949
BstMWI GCNNNNNNNGC 8 cut(s) 37, 73, 106, 115, 881, 2540, 2813, 3047
BstNI CCWGG 5 cut(s) 426, 684, 1647, 2163, 3087
BstNSI RCATGY 2 cut(s) 857, 2439
BstSCI CCNGG 5 cut(s) 424, 682, 1645, 2161, 3085
BstSFI CTRYAG 2 cut(s) 1004, 1383
BstSLI GKGCMC 1 cut(s) 3004
BstV1I GCAGC 8 cut(s) 25, 43, 85, 1259, 1997, 2429, 2855, 3059
BstV2I GAAGAC 5 cut(s) 222, 742, 2399, 2697, 3075
BstX2I RGATCY 7 cut(s) 1304, 1525, 1792, 1807, 2289, 2696, 2874
BstYI RGATCY 7 cut(s) 1304, 1525, 1792, 1807, 2289, 2696, 2874
BstZ17I GTATAC 1 cut(s) 1493
Bsu15I ATCGAT 1 cut(s) 2517
Bsu36I CCTNAGG 2 cut(s) 567, 2745
BsuRI GGCC 2 cut(s) 1333, 2832
BsuTUI ATCGAT 1 cut(s) 2517
BtgI CCRYGG 4 cut(s) 691, 948, 1900, 2808
BtsCI GGATG 6 cut(s) 85, 679, 958, 2254, 2436, 2680
BtsIMutI CAGTG 4 cut(s) 1165, 1326, 1824, 1826
BveI ACCTGC 1 cut(s) 3032
Cac8I GCNNGC 8 cut(s) 11, 102, 116, 855, 877, 1282, 2437, 3059
CaiI CAGNNNCTG 1 cut(s) 1666
CciI TCATGA 1 cut(s) 195
CfoI GCGC 1 cut(s) 810
Cfr13I GGNCC 8 cut(s) 139, 252, 688, 695, 974, 1331, 2231, 2830
ClaI ATCGAT 1 cut(s) 2517
CseI GACGC 3 cut(s) 131, 1324, 2948
CsiI ACCWGGT 1 cut(s) 3085
Csp6I GTAC 6 cut(s) 534, 1092, 1156, 1322, 2152, 2168
CspCI CAANNNNNGTGG 4 cut(s) 917, 952, 3001, 3036
CviQI GTAC 6 cut(s) 534, 1092, 1156, 1322, 2152, 2168
DraIII CACNNNGTG 1 cut(s) 1153
Eam1104I CTCTTC 2 cut(s) 342, 1947
EarI CTCTTC 2 cut(s) 342, 1947
Ecl136II GAGCTC 3 cut(s) 393, 1239, 2374
Eco130I CCWWGG 4 cut(s) 691, 1051, 1900, 2808
Eco24I GRGCYC 3 cut(s) 395, 1241, 2376
Eco31I GGTCTC 1 cut(s) 833
Eco47I GGWCC 6 cut(s) 139, 252, 688, 695, 974, 2231
Eco53kI GAGCTC 3 cut(s) 393, 1239, 2374
Eco57I CTGAAG 4 cut(s) 366, 456, 507, 2513
Eco81I CCTNAGG 2 cut(s) 567, 2745
Eco88I CYCGRG 2 cut(s) 725, 1377
EcoICRI GAGCTC 3 cut(s) 393, 1239, 2374
EcoNI CCTNNNNNAGG 1 cut(s) 2882
EcoO109I RGGNCCY 1 cut(s) 974
EcoRI GAATTC 1 cut(s) 1822
EcoRII CCWGG 5 cut(s) 424, 682, 1645, 2161, 3085
EcoT14I CCWWGG 4 cut(s) 691, 1051, 1900, 2808
EcoT22I ATGCAT 2 cut(s) 1296, 2441
EcoT38I GRGCYC 3 cut(s) 395, 1241, 2376
ErhI CCWWGG 4 cut(s) 691, 1051, 1900, 2808
FalI AAGNNNNNCTT 6 cut(s) 962, 994, 1107, 1139, 1551, 1583
FaqI GGGAC 4 cut(s) 249, 701, 960, 1605
FbaI TGATCA 2 cut(s) 198, 319
FblI GTMKAC 2 cut(s) 336, 1492
FokI GGATG 6 cut(s) 92, 666, 965, 2261, 2443, 2687
FriOI GRGCYC 3 cut(s) 395, 1241, 2376
GlaI GCGC 1 cut(s) 809
GsuI CTGGAG 4 cut(s) 594, 783, 1850, 3094
HaeII RGCGCY 1 cut(s) 811
HaeIII GGCC 2 cut(s) 1333, 2832
HapII CCGG 3 cut(s) 77, 2155, 2788
HgaI GACGC 3 cut(s) 131, 1324, 2948
HhaI GCGC 1 cut(s) 810
Hin1I GRCGYC 1 cut(s) 123
Hin6I GCGC 1 cut(s) 808
HinP1I GCGC 1 cut(s) 808
HincII GTYRAC 1 cut(s) 2737
HindII GTYRAC 1 cut(s) 2737
HindIII AAGCTT 3 cut(s) 98, 489, 1944
HpaII CCGG 3 cut(s) 77, 2155, 2788
Hpy99I CGWCG 1 cut(s) 2024
HpyAV CCTTC 9 cut(s) 246, 492, 988, 1052, 1109, 1292, 2488, 2578, 3118
HpyCH4III ACNGT 7 cut(s) 86, 247, 655, 1160, 1387, 1819, 2210
HpyCH4IV ACGT 7 cut(s) 132, 1320, 1981, 2201, 2236, 2739, 2958
HpyF10VI GCNNNNNNNGC 8 cut(s) 37, 73, 106, 115, 881, 2540, 2813, 3047
HpySE526I ACGT 7 cut(s) 132, 1320, 1981, 2201, 2236, 2739, 2958
Hsp92I GRCGYC 1 cut(s) 123
HspAI GCGC 1 cut(s) 808
KflI GGGWCCC 1 cut(s) 974
Ksp22I TGATCA 2 cut(s) 198, 319
LmnI GCTCC 2 cut(s) 2548, 2813
Lsp1109I GCAGC 8 cut(s) 25, 43, 85, 1259, 1997, 2429, 2855, 3059
LweI GCATC 4 cut(s) 529, 1234, 1281, 2530
MabI ACCWGGT 1 cut(s) 3085
MaeII ACGT 7 cut(s) 132, 1320, 1981, 2201, 2236, 2739, 2958
MfeI CAATTG 1 cut(s) 2510
MflI RGATCY 7 cut(s) 1304, 1525, 1792, 1807, 2289, 2696, 2874
MhlI GDGCHC 4 cut(s) 395, 1241, 2376, 3004
MlyI GAGTC 6 cut(s) 17, 271, 351, 1463, 1951, 3114
MmeI TCCRAC 1 cut(s) 1711
Mph1103I ATGCAT 2 cut(s) 1296, 2441
MroXI GAANNNNTTC 2 cut(s) 1119, 2594
MseI TTAA 6 cut(s) 60, 752, 776, 1106, 2126, 2559
MslI CAYNNNNRTG 2 cut(s) 1500, 2175
MspA1I CMGCKG 1 cut(s) 379
MspCI CTTAAG 1 cut(s) 59
MspI CCGG 3 cut(s) 77, 2155, 2788
MspR9I CCNGG 5 cut(s) 426, 684, 1647, 2163, 3087
MunI CAATTG 1 cut(s) 2510
Mva1269I GAATGC 2 cut(s) 1119, 1988
MvaI CCWGG 5 cut(s) 426, 684, 1647, 2163, 3087
MwoI GCNNNNNNNGC 8 cut(s) 37, 73, 106, 115, 881, 2540, 2813, 3047
NcoI CCATGG 3 cut(s) 691, 1900, 2808
NlaIV GGNNCC 7 cut(s) 254, 689, 975, 976, 1063, 1911, 2815
NmuCI GTSAC 4 cut(s) 53, 655, 1145, 1407
NsiI ATGCAT 2 cut(s) 1296, 2441
NspI RCATGY 2 cut(s) 857, 2439
OliI CACNNNNGTG 1 cut(s) 2175
PaeI GCATGC 2 cut(s) 857, 2439
PaeR7I CTCGAG 1 cut(s) 725
PagI TCATGA 1 cut(s) 195
PaqCI CACCTGC 1 cut(s) 3032
PcsI WCGNNNNNNNCGW 1 cut(s) 129
PctI GAATGC 2 cut(s) 1119, 1988
PdmI GAANNNNTTC 2 cut(s) 1119, 2594
PfeI GAWTC 7 cut(s) 183, 218, 911, 1762, 2158, 2627, 2836
PflMI CCANNNNNTGG 1 cut(s) 849
PleI GAGTC 6 cut(s) 17, 270, 351, 1462, 1951, 3113
PpsI GAGTC 6 cut(s) 17, 270, 351, 1462, 1951, 3113
Ppu21I YACGTR 3 cut(s) 133, 1321, 2202
PpuMI RGGWCCY 1 cut(s) 974
PsiI TTATAA 1 cut(s) 633
Psp124BI GAGCTC 3 cut(s) 395, 1241, 2376
Psp5II RGGWCCY 1 cut(s) 974
Psp6I CCWGG 5 cut(s) 424, 682, 1645, 2161, 3085
PspGI CCWGG 5 cut(s) 424, 682, 1645, 2161, 3085
PspN4I GGNNCC 7 cut(s) 254, 689, 975, 976, 1063, 1911, 2815
PspPI GGNCC 8 cut(s) 139, 252, 688, 695, 974, 1331, 2231, 2830
PspPPI RGGWCCY 1 cut(s) 974
PspXI VCTCGAGB 1 cut(s) 725
PstNI CAGNNNCTG 1 cut(s) 1666
PsuI RGATCY 7 cut(s) 1304, 1525, 1792, 1807, 2289, 2696, 2874
RsaI GTAC 6 cut(s) 535, 1093, 1157, 1323, 2153, 2169
RsaNI GTAC 6 cut(s) 534, 1092, 1156, 1322, 2152, 2168
RseI CAYNNNNRTG 2 cut(s) 1500, 2175
SacI GAGCTC 3 cut(s) 395, 1241, 2376
SaqAI TTAA 6 cut(s) 60, 752, 776, 1106, 2126, 2559
Sau96I GGNCC 8 cut(s) 139, 252, 688, 695, 974, 1331, 2231, 2830
ScaI AGTACT 1 cut(s) 535
SchI GAGTC 6 cut(s) 17, 271, 351, 1463, 1951, 3114
ScrFI CCNGG 5 cut(s) 426, 684, 1647, 2163, 3087
SduI GDGCHC 4 cut(s) 395, 1241, 2376, 3004
SexAI ACCWGGT 1 cut(s) 3085
SfaNI GCATC 4 cut(s) 529, 1234, 1281, 2530
SfcI CTRYAG 2 cut(s) 1004, 1383
Sfr274I CTCGAG 1 cut(s) 725
SinI GGWCC 6 cut(s) 139, 252, 688, 695, 974, 2231
SlaI CTCGAG 1 cut(s) 725
SmiMI CAYNNNNRTG 2 cut(s) 1500, 2175
SmlI CTYRAG 6 cut(s) 59, 327, 396, 725, 1616, 2293
SmoI CTYRAG 6 cut(s) 59, 327, 396, 725, 1616, 2293
SpeI ACTAGT 2 cut(s) 290, 530
SphI GCATGC 2 cut(s) 857, 2439
SsiI CCGC 5 cut(s) 71, 379, 1135, 2246, 2914
SspI AATATT 1 cut(s) 750
SstI GAGCTC 3 cut(s) 395, 1241, 2376
StyD4I CCNGG 5 cut(s) 424, 682, 1645, 2161, 3085
StyI CCWWGG 4 cut(s) 691, 1051, 1900, 2808
TaaI ACNGT 7 cut(s) 86, 247, 655, 1160, 1387, 1819, 2210
TaiI ACGT 7 cut(s) 135, 1323, 1984, 2204, 2239, 2742, 2961
TaqII GACCGA 1 cut(s) 903
TatI WGTACW 3 cut(s) 533, 1155, 2167
TauI GCSGC 3 cut(s) 382, 1138, 2917
TfiI GAWTC 7 cut(s) 183, 218, 911, 1762, 2158, 2627, 2836
Tru1I TTAA 6 cut(s) 60, 752, 776, 1106, 2126, 2559
Tru9I TTAA 6 cut(s) 60, 752, 776, 1106, 2126, 2559
TscAI CASTG 4 cut(s) 1165, 1333, 1824, 1833
TseFI GTSAC 4 cut(s) 53, 655, 1145, 1407
TseI GCWGC 8 cut(s) 13, 31, 73, 1247, 1985, 2417, 2843, 3047
Tsp45I GTSAC 4 cut(s) 53, 655, 1145, 1407
TspGWI ACGGA 3 cut(s) 16, 937, 2230
TspRI CASTG 4 cut(s) 1165, 1333, 1824, 1833
Van91I CCANNNNNTGG 1 cut(s) 849
Vha464I CTTAAG 1 cut(s) 59
VneI GTGCAC 1 cut(s) 3000
VpaK11BI GGWCC 6 cut(s) 139, 252, 688, 695, 974, 2231
XagI CCTNNNNNAGG 1 cut(s) 2882
XbaI TCTAGA 1 cut(s) 2362
XceI RCATGY 2 cut(s) 857, 2439
XcmI CCANNNNNNNNNTGG 1 cut(s) 2324
XhoI CTCGAG 1 cut(s) 725
XmiI GTMKAC 2 cut(s) 336, 1492
XmnI GAANNNNTTC 2 cut(s) 1119, 2594
ZrmI AGTACT 1 cut(s) 535
Zsp2I ATGCAT 2 cut(s) 1296, 2441
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.