FvH4_1g05990
ERF Family

Probably involved in the RNA silencing pathway and required for the generation of small interfering RNAs (siRNAs)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0000505
Physical Location & Seq
Forward (+)
3194926 .. 3204345
9420 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g05990.t1

Sequence Viewer

Length: 3264 bp
ATGTTCGGCGTGACGCCGCTACCCAACTCCGTCCAAGAAGCACTGAACCGGCTATGCGAAGTCAAGAAACAGCCTCAACCCGACGAAGGCGTCAGAAGAAAGCTGTCACTCTTGAGCAAGCAAGACGCTCTCTACGTTCTGGAACAAATCCACAACGACAGAGCTCCCATCAAAAACACCCTCGGTGCATTCATTGTCTTCATGATCGGGAAACTAGAGAATGCTTCGTCTTCGTCGTCTTCTTCTCCGAGATCAGTTAAGACTGCTTCTTCCTATCAAATCTCACCCTCTGCTTCTTCCTCCTCTCAAGAGCACCATCAAGATTATGATCGAGAGCCGCTCTATTCTTCAGCTTCATATCAGAGCTATTCTTGTCCTTCTGTTACTGATGCACCATTTTATTCAAGTTTTCAGTCTCAGGGTGAGACTTCCAATCCATTGCCGGCGACATTGTGCAATGGAAAATCACTGTGTTCCTCCCCTTCTCCCTATGAAATCCTGTCTGCTCCTTCTGATGCTTATAGACAATTCTGTGAGAGGTTCCAAGGTGTCCACATTTCTCCTTTTCAAACCCCATCTGCCTCTCCTGTCACTCCTGTACAACTTTATAAGAGTTTTCAAGGTGAGACTGTCGCTCACTCCCCAGTTCTATACCATCCTGCAGACCATGGACAGGCACACTTAGAAGCTCTGGGAGAGCTCGAATTCAGAAAACAATTTCTGATATTAAGTTATGCTGGAGGAATAAAGTTAAAAGATGTAATAGACGCGGAGGATATTAGGAATTGGAAAAATTTGCCAATGGTGAAGTTCGAGACCACGGTTTGGCATGTCTTGGGTGACGAAAATGTTGGCAAGGCTGACCGACTATTGAATGTTGATTGGGATTCCGGACGCACACATTTCTATGAATGCCATGTTACCAGAGATGGGAGTTACACATTCAAGGGCCCCTATATAAAGCATGAACGAAGAACTCTCTTACAGAAACTATCTGGAGATGACAACGTTTTGATTGTGAAGTTTGACGGAGAAGGAACTAAGATAGGTTCCAGTAATTTTTATGATAACGACTATTGTACGTATATGAAGCTTGAAAGAGAAGGGATTTTTGTTGGTTTGCGGCGATATCGTTTCTTTGGTTATAAAGATGGCGGTAAAGTAGATAAAATCAGAACCCCCTCATCATCACCTGTGAAATGTTATTTTGTCCTTGTGAGCTCTGATGCAGCAATAGACAGGGATGTAGATTATTGGTTATCTAACAGAACTATTCATGAAGGAAGACGTCTAATCATGCATATTGATACTCTGCCAAGTGTGGACAAAGTCATGGCTAGGTTGCCTCTCATTTTTTCTAAGACAACGAGCTTGGAATTGGATTGGTCAGTCGTGAAGGTTGAAATAATTGATGACATCTACTGTCTCGATGATTCTGGTAACCGTGTTTATAACAGAGATGGAAAAGCTCTTATACACACAGATGGAACTGGATTCATATCTGAGGATTTGGCGTTGCTTTGTCCAAGGATTGCCAAAAGAAAGTTCACGAGTGAGGAACACATGGAGGGACTTCTGGATCCTGATGAACTTGAGATTGAAATGACAAAATCAGATTTTCGAACTCAAGAAGCGCCATTGCTCATACAGTGCCGCCTATTTCACGAAGGCACTGCTGCTAAAGGAACTTTTACTGTCAATAAGCTGCTTCCCCCGAAAACACTGCAGATTCGTCCGTCAATGATTAAAGTTGAGAAGGACCCTAAGTTTTCAGATACTAACACCATCAATTCTCTGGAAATAGTATGCACAAGTGCTCCTCCTATGAAAGCTTATTTGTCAAGGACTATAATTGCACTTCTAGTGTATGGAGGGGTACCAAAAGACCTTTTTATGAAGATACTTGAGAATGCTGTAGAAGATGCCCATCATGTAATCAGCAAAACACGTGCAGCACTCAAAGTTGCTGAGAAGTATGGTGGGATTGATGACTTCTTGGTGGAAACAATGATCAGACTTGGTATTCCTATAGAAGAATCATATCTGCAATATCGTCTGTCATGCCTACTGCGTCTGCAGGAAGGCACTGGTCTCAGAAAAGGGAGACTTCCTATCCCTGACACTTTTATGTTGATGGGGACAGCAGATCCGACAGGGACTCTGGAAAGAGATGAAGTCTGTGTTATCCTCAAGGACGGACAACTGTCGGGGACCGTACTAGTGTACCGGCATCCTGGTCAACACTTTGGGGATATCAAAGTTTTGAAGGCTACCTACGTGAAGGAGTTGGAATCTGTTGTGGGAGATGCCAGATATGCTATATTCTTCTCTTGCAAGGGACCACGTAATGTTGCTGATGAAATTGCTGGTGGAGATTTTGATGGTGATCTCTATTGGGTCTCTAGAAACCCTCAGCTATTAAAATGGTACAAACCAAGTGAACCTTGGAACGAAGGAGAAGTAGCAACTCAAAAGGTTGACAGTAAAAGACCAACTGAGCTTTCTGATGAAGAACTTCAAGCTGAAATGTTTAGATTGTTCTTGAAAAATCGGTTTCAACCATGTTTTGCTACGGGCGAGGCAGCTAATAACTGGATTGCTTTGATGGACAAGTTCTTGACTTTGGGAGATAGTCAGGAGAAGAAGGACATAATGAACAAGATACTGCGGTTGGTTGATATATACTATATAGCTCTAGATGCCGCAAAGAAAGGTGTAAAGGTTGAAGTTCCCAAAGATTTGAAGGTGCCACGTTTTCCTCACTACATGGACAAGCTAAAGGAAGAAAAAACTGAGACTTCATCTTCCATTTTGGGAGAAATTTATGATGGAGTTGGTAAATGTCAAGCAAAACTAGCTTTTCTCACAGAAATCAAGAAACTTCCTATTTTCGACATTAATCAAGTCCCCGAACACTGCTTGAACAACTGGAGGAAACTTTACGAACAGTACAGGGGTGAGATGAGCTCGGCCATGAAAAACGCTAATAAAGATCAGGCTGCTAGTAGCGTCATAGGATATTATAAAGAGAAGCTGTATGATGCTGCTGACTTTGAGCACAGTTCAAAGCCGATTGATGCAATACTGGACGAGGCGCACGCAATATATCATGTGTGCTATGACTATGCTATCAATAAAAGAGATGTGCAAAGATGTTCCTTTGCATGGAAAGTAGCTGGTAAAGCTTTGTGCGAGCTTCACGCCAAGAAGACGGGTGAGAAGTGGTTAAGCATTGCTCCTTCTGTTTTACAACAGCTCTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RdRP

Protein Analysis

1088

Amino Acids

122.66

Weight (kDa)

6.35

Isoelectric Point (pI)

40.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RdRP_helical PF26252 227 - 291 1.5e-07 RNA dependent RNA polymerase, helical domain
RdRP PF05183 313 - 944 1.5e-99 RNA dependent RNA polymerase
RdRP_head PF26253 992 - 1070 6.4e-12 RdRP, head domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000505)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G19910 AT2G19910 AT2G19920 AT2G19920 AT2G19920 AT2G19920 AT2G19930 AT2G19930 AT2G19930
fragaria_vesca FvH4_1g05980 FvH4_1g05990 FvH4_1g05990 FvH4_1g09718
malus_domestica MD02G1064400.v1.1 MD15G1195300.v1.1 MD15G1195400.v1.1
prunus_persica Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1
pyrus_communis pycom02g05070 pycom15g17460
rosa_chinensis RchiOBHm_Chr2g0091671 RchiOBHm_Chr2g0091681 RchiOBHm_Chr2g0091691 RchiOBHm_Chr2g0091701 RchiOBHm_Chr2g0091711 RchiOBHm_Chr2g0091721 RchiOBHm_Chr2g0091731 RchiOBHm_Chr5g0014401
rosa_laevigata RLG00000013772 RLG00000016242 RLG00000016244 RLG00000016245 RLG00000016246 RLG00000032105
rosa_multiflora Rmu_co8374041.1_g000001 Rmu_co8478121.1_g000001 Rmu_sc0000897.1_g000005 Rmu_sc0000897.1_g000009
rosa_roxburghii Rroxscaffold_1G00062180 Rroxscaffold_1G00062190 Rroxscaffold_1G00068800 Rroxscaffold_2G00149790 Rroxscaffold_2G00149800 Rroxscaffold_2G00149810
rosa_rugosa Rorug02G0019600 Rorug02G0019700 Rorug02G0019800 Rorug02G0019900 Rorug02G0019900 Rorug02G0020000 Rorug02G0020000 Rorug02G0020100 Rorug02G0020100 Rorug02G0020200 Rorug05G0017800
rosa_samantha Rh2AG064700 Rh2AG064800 Rh2AG064900 Rh2BG063800 Rh2BG063900 Rh2BG064000 Rh2BG064100 Rh2CG065500 Rh2CG065600 Rh2CG065700 Rh2DG063900 Rh2DG064000 Rh2DG064100 Rh2DG064200 Rh2DG381700 Rh5AG056700 Rh5AG056800 Rh5AG056900 Rh5AG112600 Rh5BG109300 Rh5CG063800 Rh5CG121100 Rh5DG053100 Rh5DG108300 Rh6CG100100
rosa_wichuraiana Rw2G005560 Rw2G005580 Rw2G005590 Rw5G005370 Rw5G009770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 4 cut(s) 609, 1146, 1452, 3024
AasI GACNNNNNNGTC 1 cut(s) 89
AatII GACGTC 1 cut(s) 1291
Acc65I GGTACC 1 cut(s) 1876
AccB1I GGYRCC 2 cut(s) 1876, 2746
AccB7I CCANNNNNTGG 1 cut(s) 825
AccBSI CCGCTC 1 cut(s) 340
AccII CGCG 1 cut(s) 770
AccIII TCCGGA 1 cut(s) 890
AciI CCGC 8 cut(s) 17, 338, 770, 1123, 1155, 1654, 2668, 2703
AclI AACGTT 1 cut(s) 1008
AclWI GGATC 3 cut(s) 1574, 1587, 2141
AcoI YGGCCR 1 cut(s) 2970
AcsI RAATTY 3 cut(s) 704, 793, 2820
AcuI CTGAAG 1 cut(s) 333
AcvI CACGTG 1 cut(s) 1949
AcyI GRCGYC 3 cut(s) 14, 90, 1288
AfaI GTAC 7 cut(s) 600, 1081, 1878, 2217, 2225, 2429, 2951
AfiI CCNNNNNNNGG 5 cut(s) 86, 673, 825, 930, 3165
AflIII ACRYGT 1 cut(s) 1946
AhlI ACTAGT 1 cut(s) 2218
AjnI CCWGG 1 cut(s) 2233
AjuI GAANNNNNNNTTGG 4 cut(s) 1529, 1561, 2485, 2517
Alw21I GWGCWC 7 cut(s) 166, 315, 702, 1223, 1819, 2969, 3060
Alw26I GTCTC 9 cut(s) 419, 420, 620, 809, 1430, 2096, 2098, 2404, 2789
AlwI GGATC 3 cut(s) 1574, 1587, 2141
Aor13HI TCCGGA 1 cut(s) 890
AoxI GGCC 2 cut(s) 949, 2970
ApaI GGGCCC 1 cut(s) 953
ApeKI GCWGC 7 cut(s) 1229, 1676, 1705, 1952, 2582, 2999, 3044
ApoI RAATTY 3 cut(s) 704, 793, 2820
AseI ATTAAT 1 cut(s) 2898
Asp700I GAANNNNTTC 1 cut(s) 2514
Asp718I GGTACC 1 cut(s) 1876
AspLEI GCGC 2 cut(s) 1636, 3097
AspS9I GGNCC 5 cut(s) 949, 950, 1759, 2211, 2339
AsuHPI GGTGA 9 cut(s) 276, 434, 635, 817, 851, 1182, 2396, 2969, 3227
AsuII TTCGAA 1 cut(s) 1621
AvaII GGWCC 3 cut(s) 1759, 2211, 2339
BaeGI GKGCMC 1 cut(s) 953
BaeI ACNNNNGTAYC 4 cut(s) 2207, 2240, 2654, 2687
BamHI GGATCC 1 cut(s) 1579
BanI GGYRCC 2 cut(s) 1876, 2746
BanII GRGCYC 5 cut(s) 166, 702, 953, 1223, 2969
BarI GAAGNNNNNNTAC 2 cut(s) 2258, 2290
BauI CACGAG 1 cut(s) 1549
BbrPI CACGTG 1 cut(s) 1949
BbsI GAAGAC 5 cut(s) 190, 222, 231, 1291, 3215
Bbv12I GWGCWC 7 cut(s) 166, 315, 702, 1223, 1819, 2969, 3060
BbvCI CCTCAGC 1 cut(s) 2412
BbvI GCAGC 7 cut(s) 1241, 1663, 1692, 1964, 2594, 2986, 3031
BcgI CGANNNNNNTGC 4 cut(s) 1655, 1689, 1705, 1739
BciT130I CCWGG 1 cut(s) 2235
BclI TGATCA 1 cut(s) 2010
BcoDI GTCTC 9 cut(s) 419, 420, 620, 809, 1430, 2096, 2098, 2404, 2789
BcuI ACTAGT 1 cut(s) 2218
BfaI CTAG 8 cut(s) 215, 1338, 1862, 2219, 2403, 2696, 2855, 3003
BfmI CTRYAG 5 cut(s) 660, 1724, 1914, 2028, 2075
BfoI RGCGCY 1 cut(s) 1637
Bme1390I CCNGG 1 cut(s) 2235
Bme18I GGWCC 3 cut(s) 1759, 2211, 2339
BmgT120I GGNCC 5 cut(s) 949, 950, 1759, 2211, 2339
BmrFI CCNGG 1 cut(s) 2235
BmrI ACTGGG 1 cut(s) 638
BmsI GCATC 9 cut(s) 379, 505, 1216, 1912, 2239, 2296, 2689, 3031, 3067
BmuI ACTGGG 1 cut(s) 638
BoxI GACNNNNGTC 1 cut(s) 2203
BpiI GAAGAC 5 cut(s) 190, 222, 231, 1291, 3215
BplI GAGNNNNNCTC 4 cut(s) 324, 356, 2951, 2983
BpmI CTGGAG 3 cut(s) 759, 1017, 2950
Bpu10I CCTNAGC 1 cut(s) 2412
Bpu14I TTCGAA 1 cut(s) 1621
BpuEI CTTGAG 6 cut(s) 133, 291, 1611, 1613, 1925, 2174
BsaAI YACGTR 4 cut(s) 1083, 1949, 2278, 2345
BsaBI GATNNNNATC 4 cut(s) 327, 1498, 1926, 2385
BsaHI GRCGYC 3 cut(s) 14, 90, 1288
BsaI GGTCTC 3 cut(s) 809, 2096, 2404
BsaJI CCNNGG 6 cut(s) 181, 544, 667, 819, 1526, 2444
BsaWI WCCGGW 1 cut(s) 890
BsaXI ACNNNNNCTCC 2 cut(s) 1801, 1831
Bsc4I CCNNNNNNNGG 5 cut(s) 86, 673, 825, 930, 3165
Bse118I RCCGGY 3 cut(s) 48, 442, 2226
Bse1I ACTGG 7 cut(s) 644, 1053, 1495, 2092, 2597, 2933, 3090
Bse3DI GCAATG 4 cut(s) 437, 463, 1637, 3231
Bse8I GATNNNNATC 4 cut(s) 327, 1498, 1926, 2385
BseAI TCCGGA 1 cut(s) 890
BseBI CCWGG 1 cut(s) 2235
BseDI CCNNGG 6 cut(s) 181, 544, 667, 819, 1526, 2444
BseGI GGATG 3 cut(s) 655, 1249, 2230
BseJI GATNNNNATC 4 cut(s) 327, 1498, 1926, 2385
BseLI CCNNNNNNNGG 5 cut(s) 86, 673, 825, 930, 3165
BseMI GCAATG 4 cut(s) 437, 463, 1637, 3231
BseMII CTCAG 7 cut(s) 431, 1494, 1959, 2107, 2426, 2487, 2784
BseNI ACTGG 7 cut(s) 644, 1053, 1495, 2092, 2597, 2933, 3090
BseRI GAGGAG 2 cut(s) 292, 1809
BseSI GKGCMC 1 cut(s) 953
BseXI GCAGC 7 cut(s) 1241, 1663, 1692, 1964, 2594, 2986, 3031
BsgI GTGCAG 1 cut(s) 1971
Bsh1236I CGCG 1 cut(s) 770
BshFI GGCC 2 cut(s) 951, 2972
BshNI GGYRCC 2 cut(s) 1876, 2746
BsiHKAI GWGCWC 7 cut(s) 166, 315, 702, 1223, 1819, 2969, 3060
BsiSI CCGG 4 cut(s) 49, 443, 891, 2227
BslFI GGGAC 6 cut(s) 1584, 2152, 2170, 2224, 2352, 2891
BslI CCNNNNNNNGG 5 cut(s) 86, 673, 825, 930, 3165
BsmAI GTCTC 9 cut(s) 419, 420, 620, 809, 1430, 2096, 2098, 2404, 2789
BsmFI GGGAC 6 cut(s) 1584, 2152, 2170, 2224, 2352, 2891
BsmI GAATGC 4 cut(s) 188, 226, 917, 1915
BsnI GGCC 2 cut(s) 951, 2972
Bso31I GGTCTC 3 cut(s) 809, 2096, 2404
Bsp119I TTCGAA 1 cut(s) 1621
Bsp120I GGGCCC 1 cut(s) 949
Bsp1286I GDGCHC 8 cut(s) 166, 315, 702, 953, 1223, 1819, 2969, 3060
Bsp13I TCCGGA 1 cut(s) 890
Bsp1407I TGTACA 1 cut(s) 598
Bsp143I GATC 8 cut(s) 204, 251, 328, 1579, 2010, 2146, 2386, 2992
Bsp19I CCATGG 1 cut(s) 667
BspACI CCGC 8 cut(s) 17, 338, 770, 1123, 1155, 1654, 2668, 2703
BspANI GGCC 2 cut(s) 951, 2972
BspCNI CTCAG 7 cut(s) 430, 1495, 1960, 2106, 2425, 2488, 2785
BspEI TCCGGA 1 cut(s) 890
BspFNI CGCG 1 cut(s) 770
BspHI TCATGA 2 cut(s) 201, 1276
BspMAI CTGCAG 3 cut(s) 664, 1728, 2079
BspPI GGATC 3 cut(s) 1574, 1587, 2141
BspT104I TTCGAA 1 cut(s) 1621
BspT107I GGYRCC 2 cut(s) 1876, 2746
BspTNI GGTCTC 3 cut(s) 809, 2096, 2404
BsrBI CCGCTC 1 cut(s) 340
BsrDI GCAATG 4 cut(s) 437, 463, 1637, 3231
BsrFI RCCGGY 3 cut(s) 48, 442, 2226
BsrGI TGTACA 1 cut(s) 598
BsrI ACTGG 7 cut(s) 644, 1053, 1495, 2092, 2597, 2933, 3090
BssAI RCCGGY 3 cut(s) 48, 442, 2226
BssECI CCNNGG 6 cut(s) 181, 544, 667, 819, 1526, 2444
BssMI GATC 8 cut(s) 204, 251, 328, 1579, 2010, 2146, 2386, 2992
BssNI GRCGYC 3 cut(s) 14, 90, 1288
BssSI CACGAG 1 cut(s) 1549
BssT1I CCWWGG 4 cut(s) 544, 667, 1526, 2444
Bst2BI CACGAG 1 cut(s) 1549
Bst2UI CCWGG 1 cut(s) 2235
BstACI GRCGYC 3 cut(s) 14, 90, 1288
BstAUI TGTACA 1 cut(s) 598
BstBAI YACGTR 4 cut(s) 1083, 1949, 2278, 2345
BstBI TTCGAA 1 cut(s) 1621
BstC8I GCNNGC 4 cut(s) 119, 444, 3099, 3194
BstDSI CCRYGG 2 cut(s) 667, 819
BstEII GGTNACC 1 cut(s) 1439
BstF5I GGATG 3 cut(s) 655, 1249, 2230
BstFNI CGCG 1 cut(s) 770
BstH2I RGCGCY 1 cut(s) 1637
BstHHI GCGC 2 cut(s) 1636, 3097
BstKTI GATC 8 cut(s) 207, 254, 331, 1582, 2013, 2149, 2389, 2995
BstMAI GTCTC 9 cut(s) 419, 420, 620, 809, 1430, 2096, 2098, 2404, 2789
BstMBI GATC 8 cut(s) 204, 251, 328, 1579, 2010, 2146, 2386, 2992
BstMWI GCNNNNNNNGC 4 cut(s) 2315, 2699, 2855, 3182
BstNI CCWGG 1 cut(s) 2235
BstNSI RCATGY 1 cut(s) 833
BstPAI GACNNNNGTC 1 cut(s) 2203
BstPI GGTNACC 1 cut(s) 1439
BstSCI CCNGG 1 cut(s) 2233
BstSFI CTRYAG 5 cut(s) 660, 1724, 1914, 2028, 2075
BstSLI GKGCMC 1 cut(s) 953
BstSNI TACGTA 1 cut(s) 1083
BstUI CGCG 1 cut(s) 770
BstV1I GCAGC 7 cut(s) 1241, 1663, 1692, 1964, 2594, 2986, 3031
BstV2I GAAGAC 5 cut(s) 190, 222, 231, 1291, 3215
BstX2I RGATCY 2 cut(s) 1579, 2146
BstYI RGATCY 2 cut(s) 1579, 2146
BsuRI GGCC 2 cut(s) 951, 2972
BtgI CCRYGG 2 cut(s) 667, 819
BtsCI GGATG 3 cut(s) 655, 1249, 2230
BtsI GCAGTG 3 cut(s) 1671, 1721, 2914
BtsIMutI CAGTG 7 cut(s) 41, 467, 1655, 1671, 1721, 2085, 2914
Cac8I GCNNGC 4 cut(s) 119, 444, 3099, 3194
CciI TCATGA 2 cut(s) 201, 1276
CfoI GCGC 2 cut(s) 1636, 3097
Cfr10I RCCGGY 3 cut(s) 48, 442, 2226
Cfr13I GGNCC 5 cut(s) 949, 950, 1759, 2211, 2339
CseI GACGC 7 cut(s) 22, 79, 134, 776, 903, 2060, 2998
Csp6I GTAC 7 cut(s) 599, 1080, 1877, 2216, 2224, 2428, 2950
CviQI GTAC 7 cut(s) 599, 1080, 1877, 2216, 2224, 2428, 2950
DpnI GATC 8 cut(s) 206, 253, 330, 1581, 2012, 2148, 2388, 2994
DpnII GATC 8 cut(s) 204, 251, 328, 1579, 2010, 2146, 2386, 2992
DrdI GACNNNNNNGTC 1 cut(s) 89
DseDI GACNNNNNNGTC 1 cut(s) 89
EaeI YGGCCR 1 cut(s) 2970
Ecl136II GAGCTC 4 cut(s) 164, 700, 1221, 2967
Eco105I TACGTA 1 cut(s) 1083
Eco130I CCWWGG 4 cut(s) 544, 667, 1526, 2444
Eco24I GRGCYC 5 cut(s) 166, 702, 953, 1223, 2969
Eco31I GGTCTC 3 cut(s) 809, 2096, 2404
Eco32I GATATC 2 cut(s) 1130, 2254
Eco47I GGWCC 3 cut(s) 1759, 2211, 2339
Eco53kI GAGCTC 4 cut(s) 164, 700, 1221, 2967
Eco57I CTGAAG 1 cut(s) 333
Eco72I CACGTG 1 cut(s) 1949
Eco91I GGTNACC 1 cut(s) 1439
EcoICRI GAGCTC 4 cut(s) 164, 700, 1221, 2967
EcoO109I RGGNCCY 3 cut(s) 949, 950, 1759
EcoO65I GGTNACC 1 cut(s) 1439
EcoRI GAATTC 1 cut(s) 704
EcoRII CCWGG 1 cut(s) 2233
EcoRV GATATC 2 cut(s) 1130, 2254
EcoT14I CCWWGG 4 cut(s) 544, 667, 1526, 2444
EcoT22I ATGCAT 1 cut(s) 1302
EcoT38I GRGCYC 5 cut(s) 166, 702, 953, 1223, 2969
ErhI CCWWGG 4 cut(s) 544, 667, 1526, 2444
FalI AAGNNNNNCTT 4 cut(s) 2091, 2123, 2428, 2460
FaqI GGGAC 6 cut(s) 1584, 2152, 2170, 2224, 2352, 2891
FbaI TGATCA 1 cut(s) 2010
FokI GGATG 3 cut(s) 642, 1256, 2217
FriOI GRGCYC 5 cut(s) 166, 702, 953, 1223, 2969
FspBI CTAG 8 cut(s) 215, 1338, 1862, 2219, 2403, 2696, 2855, 3003
GlaI GCGC 2 cut(s) 1635, 3096
GsuI CTGGAG 3 cut(s) 759, 1017, 2950
HaeII RGCGCY 1 cut(s) 1637
HaeIII GGCC 2 cut(s) 951, 2972
HapII CCGG 4 cut(s) 49, 443, 891, 2227
HgaI GACGC 7 cut(s) 22, 79, 134, 776, 903, 2060, 2998
HhaI GCGC 2 cut(s) 1636, 3097
Hin1I GRCGYC 3 cut(s) 14, 90, 1288
Hin6I GCGC 2 cut(s) 1634, 3095
HinP1I GCGC 2 cut(s) 1634, 3095
HincII GTYRAC 2 cut(s) 2240, 2479
HindII GTYRAC 2 cut(s) 2240, 2479
HindIII AAGCTT 3 cut(s) 1091, 1830, 3183
HinfI GANTC 7 cut(s) 887, 1433, 1494, 1729, 2036, 2158, 2291
HpaII CCGG 4 cut(s) 49, 443, 891, 2227
HphI GGTGA 9 cut(s) 276, 434, 635, 817, 851, 1182, 2396, 2969, 3227
Hpy166II GTNNAC 7 cut(s) 553, 1324, 1548, 2224, 2240, 2441, 2479
Hpy8I GTNNAC 7 cut(s) 553, 1324, 1548, 2224, 2240, 2441, 2479
Hpy99I CGWCG 2 cut(s) 86, 238
HpyCH4IV ACGT 8 cut(s) 135, 1008, 1082, 1288, 1948, 2277, 2344, 2752
HpyF10VI GCNNNNNNNGC 4 cut(s) 2315, 2699, 2855, 3182
HpySE526I ACGT 8 cut(s) 135, 1008, 1082, 1288, 1948, 2277, 2344, 2752
Hsp92I GRCGYC 3 cut(s) 14, 90, 1288
HspAI GCGC 2 cut(s) 1634, 3095
Kpn2I TCCGGA 1 cut(s) 890
KpnI GGTACC 1 cut(s) 1880
KroI GCCGGC 1 cut(s) 442
KroNI GCCGGC 1 cut(s) 444
Ksp22I TGATCA 1 cut(s) 2010
Kzo9I GATC 8 cut(s) 204, 251, 328, 1579, 2010, 2146, 2386, 2992
LmnI GCTCC 4 cut(s) 169, 511, 1822, 3241
Lsp1109I GCAGC 7 cut(s) 1241, 1663, 1692, 1964, 2594, 2986, 3031
LweI GCATC 9 cut(s) 379, 505, 1216, 1912, 2239, 2296, 2689, 3031, 3067
MaeI CTAG 8 cut(s) 215, 1338, 1862, 2219, 2403, 2696, 2855, 3003
MaeII ACGT 8 cut(s) 135, 1008, 1082, 1288, 1948, 2277, 2344, 2752
MaeIII GTNAC 8 cut(s) 10, 105, 382, 589, 839, 919, 935, 1439
MalI GATC 8 cut(s) 206, 253, 330, 1581, 2012, 2148, 2388, 2994
MbiI CCGCTC 1 cut(s) 340
MboI GATC 8 cut(s) 204, 251, 328, 1579, 2010, 2146, 2386, 2992
MflI RGATCY 2 cut(s) 1579, 2146
MhlI GDGCHC 8 cut(s) 166, 315, 702, 953, 1223, 1819, 2969, 3060
MlyI GAGTC 1 cut(s) 2152
MmeI TCCRAC 2 cut(s) 2174, 2268
Mph1103I ATGCAT 1 cut(s) 1302
MroI TCCGGA 1 cut(s) 890
MroNI GCCGGC 1 cut(s) 442
MroXI GAANNNNTTC 1 cut(s) 2514
MseI TTAA 7 cut(s) 258, 728, 752, 1746, 2420, 2898, 3227
MslI CAYNNNNRTG 3 cut(s) 906, 1482, 2126
MspI CCGG 4 cut(s) 49, 443, 891, 2227
MspR9I CCNGG 1 cut(s) 2235
Mva1269I GAATGC 4 cut(s) 188, 226, 917, 1915
MvaI CCWGG 1 cut(s) 2235
MvnI CGCG 1 cut(s) 770
MwoI GCNNNNNNNGC 4 cut(s) 2315, 2699, 2855, 3182
NaeI GCCGGC 1 cut(s) 444
NcoI CCATGG 1 cut(s) 667
NdeII GATC 8 cut(s) 204, 251, 328, 1579, 2010, 2146, 2386, 2992
NgoMIV GCCGGC 1 cut(s) 442
NmeAIII GCCGAG 1 cut(s) 2948
NmuCI GTSAC 4 cut(s) 10, 105, 589, 839
NsiI ATGCAT 1 cut(s) 1302
NspI RCATGY 1 cut(s) 833
NspV TTCGAA 1 cut(s) 1621
PagI TCATGA 2 cut(s) 201, 1276
PcsI WCGNNNNNNNCGW 2 cut(s) 132, 233
PctI GAATGC 4 cut(s) 188, 226, 917, 1915
PdiI GCCGGC 1 cut(s) 444
PdmI GAANNNNTTC 1 cut(s) 2514
PfeI GAWTC 6 cut(s) 887, 1433, 1494, 1729, 2036, 2291
PflFI GACNNNGTC 1 cut(s) 1328
PflMI CCANNNNNTGG 1 cut(s) 825
PleI GAGTC 1 cut(s) 2152
PmaCI CACGTG 1 cut(s) 1949
PmlI CACGTG 1 cut(s) 1949
PpsI GAGTC 1 cut(s) 2152
Ppu21I YACGTR 4 cut(s) 1083, 1949, 2278, 2345
PpuMI RGGWCCY 1 cut(s) 1759
PshAI GACNNNNGTC 1 cut(s) 2203
PshBI ATTAAT 1 cut(s) 2898
PsiI TTATAA 4 cut(s) 609, 1146, 1452, 3024
Psp124BI GAGCTC 4 cut(s) 166, 702, 1223, 2969
Psp1406I AACGTT 1 cut(s) 1008
Psp5II RGGWCCY 1 cut(s) 1759
Psp6I CCWGG 1 cut(s) 2233
PspCI CACGTG 1 cut(s) 1949
PspEI GGTNACC 1 cut(s) 1439
PspGI CCWGG 1 cut(s) 2233
PspOMI GGGCCC 1 cut(s) 949
PspPI GGNCC 5 cut(s) 949, 950, 1759, 2211, 2339
PspPPI RGGWCCY 1 cut(s) 1759
PstI CTGCAG 3 cut(s) 664, 1728, 2079
PsuI RGATCY 2 cut(s) 1579, 2146
PsyI GACNNNGTC 1 cut(s) 1328
RsaI GTAC 7 cut(s) 600, 1081, 1878, 2217, 2225, 2429, 2951
RsaNI GTAC 7 cut(s) 599, 1080, 1877, 2216, 2224, 2428, 2950
RseI CAYNNNNRTG 3 cut(s) 906, 1482, 2126
SacI GAGCTC 4 cut(s) 166, 702, 1223, 2969
SaqAI TTAA 7 cut(s) 258, 728, 752, 1746, 2420, 2898, 3227
Sau3AI GATC 8 cut(s) 204, 251, 328, 1579, 2010, 2146, 2386, 2992
Sau96I GGNCC 5 cut(s) 949, 950, 1759, 2211, 2339
SchI GAGTC 1 cut(s) 2152
ScrFI CCNGG 1 cut(s) 2235
SduI GDGCHC 8 cut(s) 166, 315, 702, 953, 1223, 1819, 2969, 3060
SfaNI GCATC 9 cut(s) 379, 505, 1216, 1912, 2239, 2296, 2689, 3031, 3067
SfcI CTRYAG 5 cut(s) 660, 1724, 1914, 2028, 2075
SfuI TTCGAA 1 cut(s) 1621
SinI GGWCC 3 cut(s) 1759, 2211, 2339
SmiMI CAYNNNNRTG 3 cut(s) 906, 1482, 2126
SmlI CTYRAG 6 cut(s) 112, 306, 1592, 1626, 1904, 2189
SmoI CTYRAG 6 cut(s) 112, 306, 1592, 1626, 1904, 2189
SnaBI TACGTA 1 cut(s) 1083
SpeI ACTAGT 1 cut(s) 2218
SsiI CCGC 8 cut(s) 17, 338, 770, 1123, 1155, 1654, 2668, 2703
SspMI CTAG 8 cut(s) 215, 1338, 1862, 2219, 2403, 2696, 2855, 3003
SstI GAGCTC 4 cut(s) 166, 702, 1223, 2969
StyD4I CCNGG 1 cut(s) 2233
StyI CCWWGG 4 cut(s) 544, 667, 1526, 2444
TaiI ACGT 8 cut(s) 138, 1011, 1085, 1291, 1951, 2280, 2347, 2755
TaqI TCGA 6 cut(s) 331, 702, 813, 1428, 1621, 2892
TaqII GACCGA 1 cut(s) 879
TatI WGTACW 2 cut(s) 598, 2949
TauI GCSGC 5 cut(s) 19, 340, 1126, 1656, 2705
TfiI GAWTC 6 cut(s) 887, 1433, 1494, 1729, 2036, 2291
Tru1I TTAA 7 cut(s) 258, 728, 752, 1746, 2420, 2898, 3227
Tru9I TTAA 7 cut(s) 258, 728, 752, 1746, 2420, 2898, 3227
TscAI CASTG 7 cut(s) 48, 474, 1655, 1678, 1728, 2092, 2921
TseFI GTSAC 4 cut(s) 10, 105, 589, 839
TseI GCWGC 7 cut(s) 1229, 1676, 1705, 1952, 2582, 2999, 3044
Tsp45I GTSAC 4 cut(s) 10, 105, 589, 839
TspGWI ACGGA 4 cut(s) 19, 1044, 1725, 2211
TspRI CASTG 7 cut(s) 48, 474, 1655, 1678, 1728, 2092, 2921
Tth111I GACNNNGTC 1 cut(s) 1328
Van91I CCANNNNNTGG 1 cut(s) 825
VpaK11BI GGWCC 3 cut(s) 1759, 2211, 2339
VspI ATTAAT 1 cut(s) 2898
XapI RAATTY 3 cut(s) 704, 793, 2820
XbaI TCTAGA 2 cut(s) 2402, 2695
XceI RCATGY 1 cut(s) 833
XcmI CCANNNNNNNNNTGG 2 cut(s) 1792, 2442
XmnI GAANNNNTTC 1 cut(s) 2514
XspI CTAG 8 cut(s) 215, 1338, 1862, 2219, 2403, 2696, 2855, 3003
ZraI GACGTC 1 cut(s) 1289
Zsp2I ATGCAT 1 cut(s) 1302
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.