Rh5AG056800
ERF Family

Probably involved in the RNA silencing pathway and required for the generation of small interfering RNAs (siRNAs)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0000505
Physical Location & Seq
Forward (+)
4932781 .. 4935352
2572 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG056800.1

Sequence Viewer

Length: 636 bp
ATGCTGGAGGTTGTTAGAACGAAGTTGAGAGATCTTATAGCGGCAGATTACATTAGGCATTGGAAATATTTGCCAATGGTAACGTTTGAGAGCAAAATTTGGGAGTATCTAGGTCGAAAATGCATCAGCCTCGAAGACCGTCGATTGAATTTTGATTGGGATTCTGGAAGCACACACGTCTATCATTGTTACGTCAACGTGGATGGGAGTTATAGATTTAAGGGCCCATATTTATATAAAATAAGAAGAACGCTCCTACAGAAAAAATTAGGAGATGACAATGTTTTAATGGTGAAGTTTGCCAAAGAAGAGGTGCAGAGAGGTCAAAGTATTGCTTGCCATCATAATTATTCTATGTATATGAAGGTTGCAAGAGAGGGGATTCTTGTTGGTTTGCGGCGATATCGTTTCTCTGTGTTCAAAGATGGTGGTAATGAGGAGAAGAAAAAAAGCCCAACTTCATCACCTATGAAGTGTTACTTTGTTCGAGTGGCCTCTGATGCAGCCATAGACAAAGGTGTCAGTTATATATTATCTAACAGAACGATGTATGAAGCCAGATGTCTTTTCATGCATGCACATACTCTGGCTAGTTTCGATAACTACATGGTTAGGTATGTAACTCTTGTAGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RdRP

Protein Analysis

211

Amino Acids

24.84

Weight (kDa)

9.51

Isoelectric Point (pI)

41.8

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000505)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G19910 AT2G19910 AT2G19920 AT2G19920 AT2G19920 AT2G19920 AT2G19930 AT2G19930 AT2G19930
fragaria_vesca FvH4_1g05980 FvH4_1g05990 FvH4_1g05990 FvH4_1g09718
malus_domestica MD02G1064400.v1.1 MD15G1195300.v1.1 MD15G1195400.v1.1
prunus_persica Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1 Prupe.7G221200_v2.0.a1
pyrus_communis pycom02g05070 pycom15g17460
rosa_chinensis RchiOBHm_Chr2g0091671 RchiOBHm_Chr2g0091681 RchiOBHm_Chr2g0091691 RchiOBHm_Chr2g0091701 RchiOBHm_Chr2g0091711 RchiOBHm_Chr2g0091721 RchiOBHm_Chr2g0091731 RchiOBHm_Chr5g0014401
rosa_laevigata RLG00000013772 RLG00000016242 RLG00000016244 RLG00000016245 RLG00000016246 RLG00000032105
rosa_multiflora Rmu_co8374041.1_g000001 Rmu_co8478121.1_g000001 Rmu_sc0000897.1_g000005 Rmu_sc0000897.1_g000009
rosa_roxburghii Rroxscaffold_1G00062180 Rroxscaffold_1G00062190 Rroxscaffold_1G00068800 Rroxscaffold_2G00149790 Rroxscaffold_2G00149800 Rroxscaffold_2G00149810
rosa_rugosa Rorug02G0019600 Rorug02G0019700 Rorug02G0019800 Rorug02G0019900 Rorug02G0019900 Rorug02G0020000 Rorug02G0020000 Rorug02G0020100 Rorug02G0020100 Rorug02G0020200 Rorug05G0017800
rosa_samantha Rh2AG064700 Rh2AG064800 Rh2AG064900 Rh2BG063800 Rh2BG063900 Rh2BG064000 Rh2BG064100 Rh2CG065500 Rh2CG065600 Rh2CG065700 Rh2DG063900 Rh2DG064000 Rh2DG064100 Rh2DG064200 Rh2DG381700 Rh5AG056700 Rh5AG056800 Rh5AG056900 Rh5AG112600 Rh5BG109300 Rh5CG063800 Rh5CG121100 Rh5DG053100 Rh5DG108300 Rh6CG100100
rosa_wichuraiana Rw2G005560 Rw2G005580 Rw2G005590 Rw5G005370 Rw5G009770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 518
AciI CCGC 2 cut(s) 41, 397
AclI AACGTT 1 cut(s) 83
AcsI RAATTY 2 cut(s) 96, 148
AflIII ACRYGT 1 cut(s) 175
AgsI TTSAA 2 cut(s) 148, 421
AjiI CACGTC 1 cut(s) 178
AoxI GGCC 2 cut(s) 223, 492
ApaI GGGCCC 1 cut(s) 227
ApeKI GCWGC 1 cut(s) 503
ApoI RAATTY 2 cut(s) 96, 148
AspS9I GGNCC 2 cut(s) 223, 224
AsuHPI GGTGA 2 cut(s) 304, 456
BaeGI GKGCMC 1 cut(s) 227
BanII GRGCYC 1 cut(s) 227
BbsI GAAGAC 1 cut(s) 141
BbvI GCAGC 1 cut(s) 515
BccI CCATC 3 cut(s) 197, 348, 419
BcgI CGANNNNNNTGC 2 cut(s) 112, 146
BfaI CTAG 2 cut(s) 110, 591
BfmI CTRYAG 1 cut(s) 257
BglII AGATCT 1 cut(s) 31
BisI GCNGC 3 cut(s) 42, 398, 504
BlsI GCNGC 3 cut(s) 43, 399, 505
BmgBI CACGTC 1 cut(s) 178
BmgT120I GGNCC 2 cut(s) 223, 224
BmiI GGNNCC 1 cut(s) 225
BmsI GCATC 2 cut(s) 132, 490
BpiI GAAGAC 1 cut(s) 141
BpmI CTGGAG 1 cut(s) 26
BseGI GGATG 1 cut(s) 208
BseRI GAGGAG 1 cut(s) 452
BseSI GKGCMC 1 cut(s) 227
BseXI GCAGC 1 cut(s) 515
BsgI GTGCAG 1 cut(s) 335
BshFI GGCC 2 cut(s) 225, 494
BsnI GGCC 2 cut(s) 225, 494
Bsp120I GGGCCC 1 cut(s) 223
Bsp1286I GDGCHC 1 cut(s) 227
Bsp143I GATC 1 cut(s) 31
BspACI CCGC 2 cut(s) 41, 397
BspANI GGCC 2 cut(s) 225, 494
BspLI GGNNCC 1 cut(s) 225
BssMI GATC 1 cut(s) 31
Bst4CI ACNGT 1 cut(s) 140
Bst6I CTCTTC 1 cut(s) 303
BstC8I GCNNGC 2 cut(s) 337, 576
BstF5I GGATG 1 cut(s) 208
BstKTI GATC 1 cut(s) 34
BstMBI GATC 1 cut(s) 31
BstMWI GCNNNNNNNGC 1 cut(s) 500
BstNSI RCATGY 1 cut(s) 578
BstSFI CTRYAG 1 cut(s) 257
BstSLI GKGCMC 1 cut(s) 227
BstV1I GCAGC 1 cut(s) 515
BstV2I GAAGAC 1 cut(s) 141
BstX2I RGATCY 1 cut(s) 31
BstYI RGATCY 1 cut(s) 31
BsuRI GGCC 2 cut(s) 225, 494
BtrI CACGTC 1 cut(s) 178
BtsCI GGATG 1 cut(s) 208
Cac8I GCNNGC 2 cut(s) 337, 576
Cfr13I GGNCC 2 cut(s) 223, 224
CspCI CAANNNNNGTGG 2 cut(s) 409, 444
CviAII CATG 4 cut(s) 571, 575, 607, 633
CviJI RGCY 7 cut(s) 129, 225, 453, 494, 506, 557, 590
CviKI_1 RGCY 7 cut(s) 129, 225, 453, 494, 506, 557, 590
DpnI GATC 1 cut(s) 33
DpnII GATC 1 cut(s) 31
DrdI GACNNNNNNGTC 1 cut(s) 518
DseDI GACNNNNNNGTC 1 cut(s) 518
Eam1104I CTCTTC 1 cut(s) 303
EarI CTCTTC 1 cut(s) 303
Eco24I GRGCYC 1 cut(s) 227
Eco32I GATATC 1 cut(s) 404
EcoO109I RGGNCCY 1 cut(s) 223
EcoRV GATATC 1 cut(s) 404
EcoT22I ATGCAT 2 cut(s) 125, 576
EcoT38I GRGCYC 1 cut(s) 227
FaeI CATG 4 cut(s) 574, 578, 610, 636
FalI AAGNNNNNCTT 6 cut(s) 319, 351, 442, 474, 464, 496
FatI CATG 4 cut(s) 570, 574, 606, 632
Fnu4HI GCNGC 3 cut(s) 42, 398, 504
FokI GGATG 1 cut(s) 215
FriOI GRGCYC 1 cut(s) 227
Fsp4HI GCNGC 3 cut(s) 42, 398, 504
FspBI CTAG 2 cut(s) 110, 591
GluI GCNGC 3 cut(s) 42, 398, 504
GsuI CTGGAG 1 cut(s) 26
HaeIII GGCC 2 cut(s) 225, 494
Hin1II CATG 4 cut(s) 574, 578, 610, 636
HincII GTYRAC 1 cut(s) 196
HindII GTYRAC 1 cut(s) 196
HinfI GANTC 2 cut(s) 161, 382
HphI GGTGA 2 cut(s) 304, 456
Hpy166II GTNNAC 1 cut(s) 196
Hpy188I TCNGA 1 cut(s) 499
Hpy188III TCNNGA 1 cut(s) 165
Hpy8I GTNNAC 1 cut(s) 196
Hpy99I CGWCG 1 cut(s) 144
HpyAV CCTTC 1 cut(s) 358
HpyCH4III ACNGT 1 cut(s) 140
HpyCH4IV ACGT 4 cut(s) 83, 177, 192, 198
HpyCH4V TGCA 6 cut(s) 123, 316, 371, 503, 574, 578
HpyF10VI GCNNNNNNNGC 1 cut(s) 500
HpySE526I ACGT 4 cut(s) 83, 177, 192, 198
Hsp92II CATG 4 cut(s) 574, 578, 610, 636
Kzo9I GATC 1 cut(s) 31
LmnI GCTCC 1 cut(s) 258
LpnPI CCDG 3 cut(s) 150, 571, 572
Lsp1109I GCAGC 1 cut(s) 515
LweI GCATC 2 cut(s) 132, 490
MaeI CTAG 2 cut(s) 110, 591
MaeII ACGT 4 cut(s) 83, 177, 192, 198
MaeIII GTNAC 4 cut(s) 79, 188, 476, 619
MalI GATC 1 cut(s) 33
MboI GATC 1 cut(s) 31
MboII GAAGA 4 cut(s) 146, 258, 320, 454
MflI RGATCY 1 cut(s) 31
MhlI GDGCHC 1 cut(s) 227
MluCI AATT 4 cut(s) 96, 148, 266, 346
MnlI CCTC 6 cut(s) 140, 304, 314, 370, 430, 505
Mph1103I ATGCAT 2 cut(s) 125, 576
MseI TTAA 2 cut(s) 219, 287
MwoI GCNNNNNNNGC 1 cut(s) 500
NdeII GATC 1 cut(s) 31
NlaIII CATG 4 cut(s) 574, 578, 610, 636
NlaIV GGNNCC 1 cut(s) 225
NsiI ATGCAT 2 cut(s) 125, 576
NspI RCATGY 1 cut(s) 578
PaeI GCATGC 1 cut(s) 578
PfeI GAWTC 2 cut(s) 161, 382
PkrI GCNGC 3 cut(s) 43, 399, 505
Psp1406I AACGTT 1 cut(s) 83
PspN4I GGNNCC 1 cut(s) 225
PspOMI GGGCCC 1 cut(s) 223
PspPI GGNCC 2 cut(s) 223, 224
PsuI RGATCY 1 cut(s) 31
SaqAI TTAA 2 cut(s) 219, 287
SatI GCNGC 3 cut(s) 42, 398, 504
Sau3AI GATC 1 cut(s) 31
Sau96I GGNCC 2 cut(s) 223, 224
SduI GDGCHC 1 cut(s) 227
SfaNI GCATC 2 cut(s) 132, 490
SfcI CTRYAG 1 cut(s) 257
SphI GCATGC 1 cut(s) 578
Sse9I AATT 4 cut(s) 96, 148, 266, 346
SsiI CCGC 2 cut(s) 41, 397
SspI AATATT 1 cut(s) 68
SspMI CTAG 2 cut(s) 110, 591
TaaI ACNGT 1 cut(s) 140
TaiI ACGT 4 cut(s) 86, 180, 195, 201
TaqI TCGA 5 cut(s) 115, 132, 142, 487, 597
TasI AATT 4 cut(s) 96, 148, 266, 346
TauI GCSGC 2 cut(s) 44, 400
TfiI GAWTC 2 cut(s) 161, 382
Tru1I TTAA 2 cut(s) 219, 287
Tru9I TTAA 2 cut(s) 219, 287
TseI GCWGC 1 cut(s) 503
TspDTI ATGAA 5 cut(s) 377, 450, 485, 559, 567
XapI RAATTY 2 cut(s) 96, 148
XceI RCATGY 1 cut(s) 578
XspI CTAG 2 cut(s) 110, 591
Zsp2I ATGCAT 2 cut(s) 125, 576
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.