AT2G29150

Tropinone reductase homolog At1g07440-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0000208
Physical Location & Seq
Reverse (-)
12535715 .. 12536964
1250 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G29150.1

Sequence Viewer

Length: 807 bp
ATGGCTAAAGCAGGAGAAAACTCGAGAGACAAATCTAGATGGAGCCTTGAAGGCATGACCGCTCTTGTTACAGGTGGCTCCAAAGGCCTCGGAGAAGCTGTAGTGGAGGAACTAGCCATGTTGGGAGCAAGAGTTCACACATGTGCCAGAGACGAAACTCAGCTTCAAGAACGCTTACGTGAATGGCAAGCCAAAGGATTTGAGGTCACCACTTCTGTCTGCGACGTCTCTTCTCGTGAGCAACGAGAGAAACTCATGGAAACCGTTTCCTCTGTCTTCCAAGGAAAACTCAACATCCTCGTAAATAATGCGGGAACGGGTATAATAAAGCCGAGTACAGAGTATACAGCAGAAGATTACTCGTTTCTGATGGCTACAAATCTCGAGTCCGCTTTTCATCTCTCACAGATCGCGCACCCTTTGTTGAAAGCCTCTGGTTCCGGGAGCATCGTGTTCATGTCCTCTGTTGCTGGACTTGTGCATACCGGTGCATCAATCTATGGAGCATCTAAAGGAGCTATGAATCAGCTAGGAAGAAGCTTAGCATGCGAGTGGGCAAGTGACAACATAAGGGTTAACTCTGTGTGTCCATGGGTCATAACAACTCCTTTAACTAGCTTTATTTTCAGTGATGAAAAGTTAAGAAAAGCAGTGGAGGATAAGACACCAATGGGACGTGTTGGAGAAGCAAATGAAGTATCATCGCTTGTCGCATTTCTCTGTTTTCCGGCAGCTTCTTATATTACAGGTCAGACTATCTGCGTCGACGGAGGTGCTTCGGTGAATGGCTTCTCTTTCAAGCCTTAG

Protein Analysis

268

Amino Acids

28.71

Weight (kDa)

5.88

Isoelectric Point (pI)

23.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short PF00106 20 - 212 1e-50 short chain dehydrogenase
KR PF08659 20 - 177 1.8e-14 KR domain
adh_short_C2 PF13561 28 - 259 5.3e-60 Enoyl-(Acyl carrier protein) reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000208)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G07440 AT1G07440 AT2G29150 AT2G29170 AT2G29290 AT2G29290 AT2G29300 AT2G29300 AT2G29310 AT2G29310 AT2G29310 AT2G29310 AT2G29320 AT2G29320 AT2G29320 AT2G29340 AT2G29340 AT2G29340 AT2G29340 AT2G29350 AT2G29350 AT2G29350
fragaria_vesca FvH4_3g01040 FvH4_3g36954 FvH4_3g36954 FvH4_3g36955 FvH4_3g36960 FvH4_3g36960 FvH4_3g37000 FvH4_3g37000 FvH4_3g37000
malus_domestica MD03G1085500.v1.1 MD03G1085700.v1.1 MD04G1134700.v1.1 MD11G1093400.v1.1 MD11G1093500.v1.1 MD11G1093700.v1.1 MD11G1093800.v1.1 MD11G1093900.v1.1 MD11G1094300.v1.1
prunus_persica Prupe.6G068900_v2.0.a1 Prupe.6G069000_v2.0.a1 Prupe.6G069000_v2.0.a1 Prupe.6G069000_v2.0.a1 Prupe.6G069100_v2.0.a1 Prupe.6G069200_v2.0.a1 Prupe.6G069500_v2.0.a1 Prupe.6G069500_v2.0.a1 Prupe.6G069700_v2.0.a1 Prupe.6G069900_v2.0.a1 Prupe.6G070000_v2.0.a1 Prupe.6G070000_v2.0.a1 Prupe.6G070000_v2.0.a1 Prupe.6G070000_v2.0.a1 Prupe.6G070000_v2.0.a1
pyrus_communis pycom03g06770 pycom11g07900 pycom11g07910 pycom11g07920 pycom11g07940 pycom11g07950 pycom11g07970 pycom11g07990 pycom12g14220
rosa_chinensis RchiOBHm_Chr2g0131701 RchiOBHm_Chr3g0473751 RchiOBHm_Chr4g0408441 RchiOBHm_Chr4g0408481 RchiOBHm_Chr4g0408501 RchiOBHm_Chr4g0408561 RchiOBHm_Chr4g0408611 RchiOBHm_Chr4g0408661 RchiOBHm_Chr4g0408721 RchiOBHm_Chr5g0045781 RchiOBHm_Chr5g0045851 RchiOBHm_Chr5g0068421 RchiOBHm_Chr7g0222951
rosa_laevigata RLG00000008645 RLG00000008646 RLG00000008649 RLG00000035864
rosa_multiflora Rmu_sc0000774.1_g000007 Rmu_sc0001746.1_g000006 Rmu_sc0002589.1_g000024 Rmu_sc0002736.1_g000004 Rmu_sc0003677.1_g000008 Rmu_sc0005087.1_g000003 Rmu_sc0005878.1_g000021 Rmu_sc0006514.1_g000011 Rmu_sc0025386.1_g000001 Rmu_sc0032361.1_g000001
rosa_roxburghii Rroxscaffold_1G00012360 Rroxscaffold_1G00013090 Rroxscaffold_1G00035120 Rroxscaffold_3G00236380 Rroxscaffold_5G00352920 Rroxscaffold_5G00352950 Rroxscaffold_5G00352980 Rroxscaffold_5G00353000 Rroxscaffold_5G00353020
rosa_rugosa Rorug02G0295300 Rorug04G0086300 Rorug04G0094800 Rorug05G0226700 Rorug05G0378300 Rorug05G0389700 Rorug07G0212400 Rorug07G0219800 Rorug07G0219900
rosa_samantha Rh1AG094900 Rh2AG351700 Rh2BG358700 Rh2CG337200 Rh2DG376100 Rh3DG297400 Rh3DG365100 Rh4AG150600 Rh4AG150800 Rh4BG147900 Rh4BG148000 Rh4BG148100 Rh4CG158000 Rh4CG158700 Rh4CG159300 Rh4DG143600 Rh4DG143800 Rh4DG144200 Rh5AG307900 Rh5AG448500 Rh5BG316300 Rh5BG316700 Rh5BG466500 Rh5CG342400 Rh5CG488100 Rh5DG326600 Rh5DG480000 Rh7AG357400 Rh7AG406600 Rh7BG345700 Rh7CG375600 Rh7DG352300
rosa_wichuraiana Rw2G028560 Rw4G012300 Rw4G012330 Rw4G012340 Rw4G012350 Rw5G022400 Rw5G028830 Rw5G041870 Rw7G030400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 228
AccBSI CCGCTC 1 cut(s) 62
AccI GTMKAC 2 cut(s) 344, 765
AccII CGCG 1 cut(s) 413
AciI CCGC 3 cut(s) 60, 311, 390
AcyI GRCGYC 1 cut(s) 225
AfaI GTAC 1 cut(s) 337
AflIII ACRYGT 2 cut(s) 140, 676
AgeI ACCGGT 1 cut(s) 485
AgsI TTSAA 4 cut(s) 50, 167, 427, 799
AjiI CACGTC 1 cut(s) 677
AleI CACNNNNGTG 1 cut(s) 141
AloI GAACNNNNNNTCC 2 cut(s) 117, 149
AluBI AGCT 7 cut(s) 98, 163, 518, 529, 540, 618, 734
AluI AGCT 7 cut(s) 98, 163, 518, 529, 540, 618, 734
Alw26I GTCTC 3 cut(s) 21, 144, 232
Ama87I CYCGRG 2 cut(s) 22, 383
AoxI GGCC 1 cut(s) 85
ApeKI GCWGC 1 cut(s) 731
ArsI GACNNNNNNTTYG 2 cut(s) 371, 403
AsiGI ACCGGT 1 cut(s) 485
Asp700I GAANNNNTTC 1 cut(s) 788
AspLEI GCGC 1 cut(s) 415
AsuC2I CCSGG 1 cut(s) 442
AsuHPI GGTGA 2 cut(s) 199, 793
AvaI CYCGRG 2 cut(s) 22, 383
BauI CACGAG 1 cut(s) 234
BbsI GAAGAC 1 cut(s) 268
BbvI GCAGC 1 cut(s) 743
BccI CCATC 2 cut(s) 33, 364
BcgI CGANNNNNNTGC 2 cut(s) 755, 789
BcnI CCSGG 1 cut(s) 442
BcoDI GTCTC 3 cut(s) 21, 144, 232
BfaI CTAG 4 cut(s) 36, 113, 530, 615
BfmI CTRYAG 1 cut(s) 99
BglI GCCNNNNNGGC 1 cut(s) 51
BisI GCNGC 1 cut(s) 732
BlpI GCTNAGC 1 cut(s) 541
BlsI GCNGC 1 cut(s) 733
Bme1390I CCNGG 1 cut(s) 442
BmeT110I CYCGRG 2 cut(s) 22, 383
BmgBI CACGTC 1 cut(s) 677
BmiI GGNNCC 3 cut(s) 44, 79, 439
BmrFI CCNGG 1 cut(s) 442
BmsI GCATC 3 cut(s) 456, 500, 515
BpiI GAAGAC 1 cut(s) 268
BplI GAGNNNNNCTC 2 cut(s) 237, 269
Bpu1102I GCTNAGC 1 cut(s) 541
BpuMI CCSGG 1 cut(s) 442
BsaAI YACGTR 1 cut(s) 179
BsaHI GRCGYC 1 cut(s) 225
BsaJI CCNNGG 3 cut(s) 88, 280, 590
BsaWI WCCGGW 1 cut(s) 485
BsaXI ACNNNNNCTCC 4 cut(s) 84, 114, 117, 147
Bse118I RCCGGY 1 cut(s) 485
BseDI CCNNGG 3 cut(s) 88, 280, 590
BseGI GGATG 1 cut(s) 294
BseMII CTCAG 1 cut(s) 173
BseXI GCAGC 1 cut(s) 743
Bsh1236I CGCG 1 cut(s) 413
BshFI GGCC 1 cut(s) 87
BshTI ACCGGT 1 cut(s) 485
BsiHKCI CYCGRG 2 cut(s) 22, 383
BsiSI CCGG 3 cut(s) 441, 486, 728
BslFI GGGAC 1 cut(s) 687
BsmAI GTCTC 3 cut(s) 21, 144, 232
BsmBI CGTCTC 2 cut(s) 144, 232
BsmFI GGGAC 1 cut(s) 687
BsnI GGCC 1 cut(s) 87
BsoBI CYCGRG 2 cut(s) 22, 383
Bsp143I GATC 1 cut(s) 408
Bsp1720I GCTNAGC 1 cut(s) 541
Bsp19I CCATGG 1 cut(s) 590
BspACI CCGC 3 cut(s) 60, 311, 390
BspANI GGCC 1 cut(s) 87
BspCNI CTCAG 1 cut(s) 172
BspFNI CGCG 1 cut(s) 413
BspLI GGNNCC 3 cut(s) 44, 79, 439
BsrBI CCGCTC 1 cut(s) 62
BsrFI RCCGGY 1 cut(s) 485
BssAI RCCGGY 1 cut(s) 485
BssECI CCNNGG 3 cut(s) 88, 280, 590
BssMI GATC 1 cut(s) 408
BssNAI GTATAC 1 cut(s) 345
BssNI GRCGYC 1 cut(s) 225
BssSI CACGAG 1 cut(s) 234
BssT1I CCWWGG 2 cut(s) 280, 590
Bst1107I GTATAC 1 cut(s) 345
Bst2BI CACGAG 1 cut(s) 234
Bst4CI ACNGT 1 cut(s) 265
Bst6I CTCTTC 1 cut(s) 235
BstACI GRCGYC 1 cut(s) 225
BstBAI YACGTR 1 cut(s) 179
BstC8I GCNNGC 2 cut(s) 189, 547
BstDEI CTNAG 3 cut(s) 159, 541, 804
BstDSI CCRYGG 1 cut(s) 590
BstEII GGTNACC 1 cut(s) 205
BstF5I GGATG 1 cut(s) 294
BstFNI CGCG 1 cut(s) 413
BstHHI GCGC 1 cut(s) 415
BstKTI GATC 1 cut(s) 411
BstMAI GTCTC 3 cut(s) 21, 144, 232
BstMBI GATC 1 cut(s) 408
BstMWI GCNNNNNNNGC 3 cut(s) 51, 84, 546
BstNSI RCATGY 2 cut(s) 144, 549
BstPI GGTNACC 1 cut(s) 205
BstSCI CCNGG 1 cut(s) 440
BstSFI CTRYAG 1 cut(s) 99
BstUI CGCG 1 cut(s) 413
BstV1I GCAGC 1 cut(s) 743
BstV2I GAAGAC 1 cut(s) 268
BstZ17I GTATAC 1 cut(s) 345
BsuRI GGCC 1 cut(s) 87
BtgI CCRYGG 1 cut(s) 590
BtgZI GCGATG 1 cut(s) 687
BtrI CACGTC 1 cut(s) 677
BtsCI GGATG 1 cut(s) 294
BtsI GCAGTG 1 cut(s) 657
BtsIMutI CAGTG 2 cut(s) 634, 657
Cac8I GCNNGC 2 cut(s) 189, 547
CfoI GCGC 1 cut(s) 415
Cfr10I RCCGGY 1 cut(s) 485
CseI GACGC 1 cut(s) 751
Csp6I GTAC 1 cut(s) 336
CspAI ACCGGT 1 cut(s) 485
CviAII CATG 7 cut(s) 55, 118, 141, 256, 457, 546, 591
CviQI GTAC 1 cut(s) 336
DdeI CTNAG 3 cut(s) 159, 541, 804
DpnI GATC 1 cut(s) 410
DpnII GATC 1 cut(s) 408
Eam1104I CTCTTC 1 cut(s) 235
EarI CTCTTC 1 cut(s) 235
Eco130I CCWWGG 2 cut(s) 280, 590
Eco147I AGGCCT 1 cut(s) 87
Eco88I CYCGRG 2 cut(s) 22, 383
Eco91I GGTNACC 1 cut(s) 205
EcoO65I GGTNACC 1 cut(s) 205
EcoT14I CCWWGG 2 cut(s) 280, 590
ErhI CCWWGG 2 cut(s) 280, 590
Esp3I CGTCTC 2 cut(s) 144, 232
FaeI CATG 7 cut(s) 58, 121, 144, 259, 460, 549, 594
FaqI GGGAC 1 cut(s) 687
FatI CATG 7 cut(s) 54, 117, 140, 255, 456, 545, 590
FauI CCCGC 1 cut(s) 304
FblI GTMKAC 2 cut(s) 344, 765
Fnu4HI GCNGC 1 cut(s) 732
FokI GGATG 1 cut(s) 281
Fsp4HI GCNGC 1 cut(s) 732
FspBI CTAG 4 cut(s) 36, 113, 530, 615
GlaI GCGC 1 cut(s) 414
GluI GCNGC 1 cut(s) 732
HaeIII GGCC 1 cut(s) 87
HapII CCGG 3 cut(s) 441, 486, 728
HgaI GACGC 1 cut(s) 751
HhaI GCGC 1 cut(s) 415
Hin1I GRCGYC 1 cut(s) 225
Hin1II CATG 7 cut(s) 58, 121, 144, 259, 460, 549, 594
Hin6I GCGC 1 cut(s) 413
HinP1I GCGC 1 cut(s) 413
HincII GTYRAC 2 cut(s) 577, 766
HindII GTYRAC 2 cut(s) 577, 766
HindIII AAGCTT 1 cut(s) 538
HinfI GANTC 2 cut(s) 386, 523
HpaI GTTAAC 1 cut(s) 577
HpaII CCGG 3 cut(s) 441, 486, 728
HphI GGTGA 2 cut(s) 199, 793
Hpy166II GTNNAC 4 cut(s) 136, 345, 577, 766
Hpy188I TCNGA 3 cut(s) 92, 369, 753
Hpy188III TCNNGA 5 cut(s) 24, 36, 167, 236, 383
Hpy8I GTNNAC 4 cut(s) 136, 345, 577, 766
Hpy99I CGWCG 3 cut(s) 227, 767, 770
HpyAV CCTTC 1 cut(s) 44
HpyCH4III ACNGT 1 cut(s) 265
HpyCH4IV ACGT 3 cut(s) 178, 225, 676
HpyCH4V TGCA 2 cut(s) 481, 491
HpyF10VI GCNNNNNNNGC 3 cut(s) 51, 84, 546
HpyF3I CTNAG 3 cut(s) 159, 541, 804
HpySE526I ACGT 3 cut(s) 178, 225, 676
Hsp92I GRCGYC 1 cut(s) 225
Hsp92II CATG 7 cut(s) 58, 121, 144, 259, 460, 549, 594
HspAI GCGC 1 cut(s) 413
KspAI GTTAAC 1 cut(s) 577
Kzo9I GATC 1 cut(s) 408
LmnI GCTCC 6 cut(s) 42, 83, 125, 444, 503, 515
LpnPI CCDG 8 cut(s) 57, 160, 420, 454, 456, 499, 732, 741
Lsp1109I GCAGC 1 cut(s) 743
LweI GCATC 3 cut(s) 456, 500, 515
MaeI CTAG 4 cut(s) 36, 113, 530, 615
MaeII ACGT 3 cut(s) 178, 225, 676
MaeIII GTNAC 3 cut(s) 67, 205, 560
MalI GATC 1 cut(s) 410
MbiI CCGCTC 1 cut(s) 62
MboI GATC 1 cut(s) 408
MboII GAAGA 4 cut(s) 222, 268, 365, 546
MlyI GAGTC 1 cut(s) 395
MmeI TCCRAC 1 cut(s) 661
MnlI CCTC 9 cut(s) 98, 100, 196, 280, 308, 442, 472, 649, 764
MroXI GAANNNNTTC 1 cut(s) 788
MseI TTAA 3 cut(s) 576, 611, 641
MslI CAYNNNNRTG 3 cut(s) 141, 486, 550
MspI CCGG 3 cut(s) 441, 486, 728
MspR9I CCNGG 1 cut(s) 442
MvnI CGCG 1 cut(s) 413
MwoI GCNNNNNNNGC 3 cut(s) 51, 84, 546
NciI CCSGG 1 cut(s) 442
NcoI CCATGG 1 cut(s) 590
NdeII GATC 1 cut(s) 408
NlaIII CATG 7 cut(s) 58, 121, 144, 259, 460, 549, 594
NlaIV GGNNCC 3 cut(s) 44, 79, 439
NmeAIII GCCGAG 1 cut(s) 357
NmuCI GTSAC 2 cut(s) 205, 560
NspI RCATGY 2 cut(s) 144, 549
OliI CACNNNNGTG 1 cut(s) 141
PaeI GCATGC 1 cut(s) 549
PaeR7I CTCGAG 2 cut(s) 22, 383
PceI AGGCCT 1 cut(s) 87
PciI ACATGT 1 cut(s) 140
PcsI WCGNNNNNNNCGW 1 cut(s) 241
PdmI GAANNNNTTC 1 cut(s) 788
PfeI GAWTC 1 cut(s) 523
PfoI TCCNGGA 1 cut(s) 440
PinAI ACCGGT 1 cut(s) 485
PkrI GCNGC 1 cut(s) 733
PleI GAGTC 1 cut(s) 394
PpsI GAGTC 1 cut(s) 394
Ppu21I YACGTR 1 cut(s) 179
PscI ACATGT 1 cut(s) 140
PspEI GGTNACC 1 cut(s) 205
PspN4I GGNNCC 3 cut(s) 44, 79, 439
RsaI GTAC 1 cut(s) 337
RsaNI GTAC 1 cut(s) 336
RseI CAYNNNNRTG 3 cut(s) 141, 486, 550
SalI GTCGAC 1 cut(s) 764
SaqAI TTAA 3 cut(s) 576, 611, 641
SatI GCNGC 1 cut(s) 732
Sau3AI GATC 1 cut(s) 408
SchI GAGTC 1 cut(s) 395
ScrFI CCNGG 1 cut(s) 442
SfaNI GCATC 3 cut(s) 456, 500, 515
SfcI CTRYAG 1 cut(s) 99
Sfr274I CTCGAG 2 cut(s) 22, 383
SgrDI CGTCGACG 1 cut(s) 764
SlaI CTCGAG 2 cut(s) 22, 383
SmiMI CAYNNNNRTG 3 cut(s) 141, 486, 550
SmlI CTYRAG 2 cut(s) 22, 383
SmoI CTYRAG 2 cut(s) 22, 383
SphI GCATGC 1 cut(s) 549
SseBI AGGCCT 1 cut(s) 87
SsiI CCGC 3 cut(s) 60, 311, 390
SspMI CTAG 4 cut(s) 36, 113, 530, 615
StuI AGGCCT 1 cut(s) 87
StyD4I CCNGG 1 cut(s) 440
StyI CCWWGG 2 cut(s) 280, 590
TaaI ACNGT 1 cut(s) 265
TaiI ACGT 3 cut(s) 181, 228, 679
TaqI TCGA 3 cut(s) 23, 384, 765
TatI WGTACW 1 cut(s) 335
TfiI GAWTC 1 cut(s) 523
Tru1I TTAA 3 cut(s) 576, 611, 641
Tru9I TTAA 3 cut(s) 576, 611, 641
TscAI CASTG 2 cut(s) 634, 657
TseFI GTSAC 2 cut(s) 205, 560
TseI GCWGC 1 cut(s) 731
Tsp45I GTSAC 2 cut(s) 205, 560
TspDTI ATGAA 5 cut(s) 386, 445, 536, 648, 708
TspGWI ACGGA 1 cut(s) 783
TspRI CASTG 2 cut(s) 634, 657
XbaI TCTAGA 1 cut(s) 35
XceI RCATGY 2 cut(s) 144, 549
XhoI CTCGAG 2 cut(s) 22, 383
XmiI GTMKAC 2 cut(s) 344, 765
XmnI GAANNNNTTC 1 cut(s) 788
XspI CTAG 4 cut(s) 36, 113, 530, 615
ZraI GACGTC 1 cut(s) 226
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.