Rroxscaffold_5G00353000

Tropinone reductase homolog At1g07440-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0000208
Physical Location & Seq
Reverse (-)
30443034 .. 30447696
4663 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00353000.1

Sequence Viewer

Length: 645 bp
ATGGATAAATACAACTTGAGAGAGAAGGGGCATGCTCGGCATCGGACTTATGCTTTGGGAATCAAAGAGATTTGGGAAAAGACTCGAGCGTGTACGCTTGGAATTGGACAGAATGGTATCAAAGTTTGTGCGAGTAACGGTTTCAATTATATTTTCGATGGTGTTTTATTCTCATATGCTATTGTGGAGGAATTGGCGAGGCTAGGTGCAAAGGTCCATACTTGTGCTCGGAATGAAGTCCAACTCAATGAGCGCTTGAGTCAATGGAAGACCAAGGGTTTTCATCAAGTCACTGGTTCTGTATGTGATTTGATCTCAAAGCCCCAACGAGAAGAGCTCATAAACAAGGTCTCATCACTGTTCAATGGCAAACTAAACATCCTTATAAACAACGTGGGAACCAATATAGCAAAGCCCACAACTGATTACACAGCTGAAGATTATTCATTTCTTATGGGAACCAATCTTGAATCTTATTACAATATGTGCCAACTTGCACATCCTCTCCTCAAAGCTTCAGGAGCTGGTAATATTGTTCTTATATCTTCTATTGCTGGTGTGATATCAATTGGGGGTAGTGGAAGTGTCTATGCTGCAACTAAAGGTCTCTTTTTCCTAACCTACCCTTTGACTCTTCAATATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00960 ko01100 ko01110 map00960 map01100 map01110
Pfam Domains
Protein Families
adh_short adh_short_C2

Protein Analysis

214

Amino Acids

23.6

Weight (kDa)

9.03

Isoelectric Point (pI)

24.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short PF00106 59 - 202 3.5e-26 short chain dehydrogenase
adh_short_C2 PF13561 59 - 202 4.7e-21 Enoyl-(Acyl carrier protein) reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000208)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G07440 AT1G07440 AT2G29150 AT2G29170 AT2G29290 AT2G29290 AT2G29300 AT2G29300 AT2G29310 AT2G29310 AT2G29310 AT2G29310 AT2G29320 AT2G29320 AT2G29320 AT2G29340 AT2G29340 AT2G29340 AT2G29340 AT2G29350 AT2G29350 AT2G29350
fragaria_vesca FvH4_3g01040 FvH4_3g36954 FvH4_3g36954 FvH4_3g36955 FvH4_3g36960 FvH4_3g36960 FvH4_3g37000 FvH4_3g37000 FvH4_3g37000
malus_domestica MD03G1085500.v1.1 MD03G1085700.v1.1 MD04G1134700.v1.1 MD11G1093400.v1.1 MD11G1093500.v1.1 MD11G1093700.v1.1 MD11G1093800.v1.1 MD11G1093900.v1.1 MD11G1094300.v1.1
prunus_persica Prupe.6G068900_v2.0.a1 Prupe.6G069000_v2.0.a1 Prupe.6G069000_v2.0.a1 Prupe.6G069000_v2.0.a1 Prupe.6G069100_v2.0.a1 Prupe.6G069200_v2.0.a1 Prupe.6G069500_v2.0.a1 Prupe.6G069500_v2.0.a1 Prupe.6G069700_v2.0.a1 Prupe.6G069900_v2.0.a1 Prupe.6G070000_v2.0.a1 Prupe.6G070000_v2.0.a1 Prupe.6G070000_v2.0.a1 Prupe.6G070000_v2.0.a1 Prupe.6G070000_v2.0.a1
pyrus_communis pycom03g06770 pycom11g07900 pycom11g07910 pycom11g07920 pycom11g07940 pycom11g07950 pycom11g07970 pycom11g07990 pycom12g14220
rosa_chinensis RchiOBHm_Chr2g0131701 RchiOBHm_Chr3g0473751 RchiOBHm_Chr4g0408441 RchiOBHm_Chr4g0408481 RchiOBHm_Chr4g0408501 RchiOBHm_Chr4g0408561 RchiOBHm_Chr4g0408611 RchiOBHm_Chr4g0408661 RchiOBHm_Chr4g0408721 RchiOBHm_Chr5g0045781 RchiOBHm_Chr5g0045851 RchiOBHm_Chr5g0068421 RchiOBHm_Chr7g0222951
rosa_laevigata RLG00000008645 RLG00000008646 RLG00000008649 RLG00000035864
rosa_multiflora Rmu_sc0000774.1_g000007 Rmu_sc0001746.1_g000006 Rmu_sc0002589.1_g000024 Rmu_sc0002736.1_g000004 Rmu_sc0003677.1_g000008 Rmu_sc0005087.1_g000003 Rmu_sc0005878.1_g000021 Rmu_sc0006514.1_g000011 Rmu_sc0025386.1_g000001 Rmu_sc0032361.1_g000001
rosa_roxburghii Rroxscaffold_1G00012360 Rroxscaffold_1G00013090 Rroxscaffold_1G00035120 Rroxscaffold_3G00236380 Rroxscaffold_5G00352920 Rroxscaffold_5G00352950 Rroxscaffold_5G00352980 Rroxscaffold_5G00353000 Rroxscaffold_5G00353020
rosa_rugosa Rorug02G0295300 Rorug04G0086300 Rorug04G0094800 Rorug05G0226700 Rorug05G0378300 Rorug05G0389700 Rorug07G0212400 Rorug07G0219800 Rorug07G0219900
rosa_samantha Rh1AG094900 Rh2AG351700 Rh2BG358700 Rh2CG337200 Rh2DG376100 Rh3DG297400 Rh3DG365100 Rh4AG150600 Rh4AG150800 Rh4BG147900 Rh4BG148000 Rh4BG148100 Rh4CG158000 Rh4CG158700 Rh4CG159300 Rh4DG143600 Rh4DG143800 Rh4DG144200 Rh5AG307900 Rh5AG448500 Rh5BG316300 Rh5BG316700 Rh5BG466500 Rh5CG342400 Rh5CG488100 Rh5DG326600 Rh5DG480000 Rh7AG357400 Rh7AG406600 Rh7BG345700 Rh7CG375600 Rh7DG352300
rosa_wichuraiana Rw2G028560 Rw4G012300 Rw4G012330 Rw4G012340 Rw4G012350 Rw5G022400 Rw5G028830 Rw5G041870 Rw7G030400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 386
AcuI CTGAAG 2 cut(s) 456, 501
AfaI GTAC 1 cut(s) 94
AfeI AGCGCT 1 cut(s) 254
AgsI TTSAA 4 cut(s) 145, 364, 470, 638
AluBI AGCT 4 cut(s) 337, 434, 515, 524
AluI AGCT 4 cut(s) 337, 434, 515, 524
Alw21I GWGCWC 2 cut(s) 229, 339
Alw26I GTCTC 2 cut(s) 355, 611
AlwNI CAGNNNCTG 1 cut(s) 524
Ama87I CYCGRG 1 cut(s) 84
Aor51HI AGCGCT 1 cut(s) 254
ApeKI GCWGC 1 cut(s) 593
ArsI GACNNNNNNTTYG 2 cut(s) 37, 69
AspLEI GCGC 1 cut(s) 255
AspS9I GGNCC 1 cut(s) 214
AvaI CYCGRG 1 cut(s) 84
AvaII GGWCC 1 cut(s) 214
BanII GRGCYC 1 cut(s) 339
BbsI GAAGAC 1 cut(s) 275
Bbv12I GWGCWC 2 cut(s) 229, 339
BbvI GCAGC 1 cut(s) 580
BccI CCATC 1 cut(s) 152
BcoDI GTCTC 2 cut(s) 355, 611
BfaI CTAG 1 cut(s) 203
BfoI RGCGCY 1 cut(s) 256
BisI GCNGC 1 cut(s) 594
BlsI GCNGC 1 cut(s) 595
Bme18I GGWCC 1 cut(s) 214
BmeT110I CYCGRG 1 cut(s) 84
BmgT120I GGNCC 1 cut(s) 214
BmiI GGNNCC 2 cut(s) 400, 460
BmsI GCATC 1 cut(s) 49
BpiI GAAGAC 1 cut(s) 275
BplI GAGNNNNNCTC 2 cut(s) 321, 353
BpuEI CTTGAG 2 cut(s) 37, 277
BsaI GGTCTC 2 cut(s) 355, 611
BsaJI CCNNGG 1 cut(s) 273
BsaXI ACNNNNNCTCC 2 cut(s) 489, 519
Bse1I ACTGG 1 cut(s) 298
BseDI CCNNGG 1 cut(s) 273
BseGI GGATG 2 cut(s) 378, 499
BseNI ACTGG 1 cut(s) 298
BseRI GAGGAG 1 cut(s) 497
BseXI GCAGC 1 cut(s) 580
BsiHKAI GWGCWC 2 cut(s) 229, 339
BsiHKCI CYCGRG 1 cut(s) 84
BsmAI GTCTC 2 cut(s) 355, 611
Bso31I GGTCTC 2 cut(s) 355, 611
BsoBI CYCGRG 1 cut(s) 84
Bsp1286I GDGCHC 2 cut(s) 229, 339
Bsp143I GATC 1 cut(s) 312
BspLI GGNNCC 2 cut(s) 400, 460
BspQI GCTCTTC 1 cut(s) 327
BspTNI GGTCTC 2 cut(s) 355, 611
BsrI ACTGG 1 cut(s) 298
BssECI CCNNGG 1 cut(s) 273
BssMI GATC 1 cut(s) 312
BssT1I CCWWGG 1 cut(s) 273
Bst4CI ACNGT 2 cut(s) 140, 360
Bst6I CTCTTC 2 cut(s) 327, 639
BstC8I GCNNGC 1 cut(s) 33
BstF5I GGATG 2 cut(s) 378, 499
BstH2I RGCGCY 1 cut(s) 256
BstHHI GCGC 1 cut(s) 255
BstKTI GATC 1 cut(s) 315
BstMAI GTCTC 2 cut(s) 355, 611
BstMBI GATC 1 cut(s) 312
BstMWI GCNNNNNNNGC 2 cut(s) 37, 521
BstNSI RCATGY 1 cut(s) 35
BstV1I GCAGC 1 cut(s) 580
BstV2I GAAGAC 1 cut(s) 275
BtsCI GGATG 2 cut(s) 378, 499
BtsIMutI CAGTG 2 cut(s) 291, 356
Cac8I GCNNGC 1 cut(s) 33
CaiI CAGNNNCTG 1 cut(s) 524
CfoI GCGC 1 cut(s) 255
Cfr13I GGNCC 1 cut(s) 214
Csp6I GTAC 1 cut(s) 93
CviAII CATG 1 cut(s) 32
CviJI RGCY 7 cut(s) 202, 322, 337, 415, 434, 515, 524
CviKI_1 RGCY 7 cut(s) 202, 322, 337, 415, 434, 515, 524
CviQI GTAC 1 cut(s) 93
DpnI GATC 1 cut(s) 314
DpnII GATC 1 cut(s) 312
Eam1104I CTCTTC 2 cut(s) 327, 639
EarI CTCTTC 2 cut(s) 327, 639
Ecl136II GAGCTC 1 cut(s) 337
Eco130I CCWWGG 1 cut(s) 273
Eco24I GRGCYC 1 cut(s) 339
Eco31I GGTCTC 2 cut(s) 355, 611
Eco32I GATATC 1 cut(s) 564
Eco47I GGWCC 1 cut(s) 214
Eco47III AGCGCT 1 cut(s) 254
Eco53kI GAGCTC 1 cut(s) 337
Eco57I CTGAAG 2 cut(s) 456, 501
Eco88I CYCGRG 1 cut(s) 84
EcoICRI GAGCTC 1 cut(s) 337
EcoRV GATATC 1 cut(s) 564
EcoT14I CCWWGG 1 cut(s) 273
EcoT38I GRGCYC 1 cut(s) 339
ErhI CCWWGG 1 cut(s) 273
FaeI CATG 1 cut(s) 35
FatI CATG 1 cut(s) 31
FauNDI CATATG 1 cut(s) 175
Fnu4HI GCNGC 1 cut(s) 594
FokI GGATG 2 cut(s) 365, 486
FriOI GRGCYC 1 cut(s) 339
Fsp4HI GCNGC 1 cut(s) 594
FspBI CTAG 1 cut(s) 203
GlaI GCGC 1 cut(s) 254
GluI GCNGC 1 cut(s) 594
HaeII RGCGCY 1 cut(s) 256
HhaI GCGC 1 cut(s) 255
Hin1II CATG 1 cut(s) 35
Hin6I GCGC 1 cut(s) 253
HinP1I GCGC 1 cut(s) 253
HindIII AAGCTT 1 cut(s) 513
HinfI GANTC 5 cut(s) 60, 82, 259, 470, 631
Hpy166II GTNNAC 1 cut(s) 93
Hpy188I TCNGA 2 cut(s) 45, 231
Hpy188III TCNNGA 2 cut(s) 467, 519
Hpy8I GTNNAC 1 cut(s) 93
HpyAV CCTTC 1 cut(s) 19
HpyCH4III ACNGT 2 cut(s) 140, 360
HpyCH4IV ACGT 1 cut(s) 393
HpyCH4V TGCA 3 cut(s) 209, 497, 596
HpyF10VI GCNNNNNNNGC 2 cut(s) 37, 521
HpySE526I ACGT 1 cut(s) 393
Hsp92II CATG 1 cut(s) 35
HspAI GCGC 1 cut(s) 253
Kzo9I GATC 1 cut(s) 312
LguI GCTCTTC 1 cut(s) 327
LmnI GCTCC 1 cut(s) 521
LpnPI CCDG 4 cut(s) 279, 504, 510, 540
Lsp1109I GCAGC 1 cut(s) 580
LweI GCATC 1 cut(s) 49
MaeI CTAG 1 cut(s) 203
MaeII ACGT 1 cut(s) 393
MaeIII GTNAC 2 cut(s) 134, 289
MalI GATC 1 cut(s) 314
MboI GATC 1 cut(s) 312
MboII GAAGA 5 cut(s) 280, 344, 449, 537, 626
MfeI CAATTG 1 cut(s) 567
MhlI GDGCHC 2 cut(s) 229, 339
MluCI AATT 4 cut(s) 102, 145, 191, 567
MlyI GAGTC 3 cut(s) 76, 268, 625
MmeI TCCRAC 1 cut(s) 265
MnlI CCTC 4 cut(s) 181, 192, 513, 518
MseI TTAA 1 cut(s) 643
MslI CAYNNNNRTG 1 cut(s) 222
MspA1I CMGCKG 1 cut(s) 434
MunI CAATTG 1 cut(s) 567
MwoI GCNNNNNNNGC 2 cut(s) 37, 521
NdeI CATATG 1 cut(s) 175
NdeII GATC 1 cut(s) 312
NlaIII CATG 1 cut(s) 35
NlaIV GGNNCC 2 cut(s) 400, 460
NmeAIII GCCGAG 1 cut(s) 16
NmuCI GTSAC 1 cut(s) 289
NspI RCATGY 1 cut(s) 35
PaeI GCATGC 1 cut(s) 35
PaeR7I CTCGAG 1 cut(s) 84
PciSI GCTCTTC 1 cut(s) 327
PfeI GAWTC 2 cut(s) 60, 470
PkrI GCNGC 1 cut(s) 595
PleI GAGTC 3 cut(s) 76, 267, 625
PpsI GAGTC 3 cut(s) 76, 267, 625
PsiI TTATAA 1 cut(s) 386
Psp124BI GAGCTC 1 cut(s) 339
PspN4I GGNNCC 2 cut(s) 400, 460
PspPI GGNCC 1 cut(s) 214
PspXI VCTCGAGB 1 cut(s) 84
PstNI CAGNNNCTG 1 cut(s) 524
PvuII CAGCTG 1 cut(s) 434
RsaI GTAC 1 cut(s) 94
RsaNI GTAC 1 cut(s) 93
RseI CAYNNNNRTG 1 cut(s) 222
SacI GAGCTC 1 cut(s) 339
SapI GCTCTTC 1 cut(s) 327
SaqAI TTAA 1 cut(s) 643
SatI GCNGC 1 cut(s) 594
Sau3AI GATC 1 cut(s) 312
Sau96I GGNCC 1 cut(s) 214
SchI GAGTC 3 cut(s) 76, 268, 625
SduI GDGCHC 2 cut(s) 229, 339
SfaNI GCATC 1 cut(s) 49
Sfr274I CTCGAG 1 cut(s) 84
SinI GGWCC 1 cut(s) 214
SlaI CTCGAG 1 cut(s) 84
SmiMI CAYNNNNRTG 1 cut(s) 222
SmlI CTYRAG 3 cut(s) 16, 84, 256
SmoI CTYRAG 3 cut(s) 16, 84, 256
SphI GCATGC 1 cut(s) 35
Sse9I AATT 4 cut(s) 102, 145, 191, 567
SspI AATATT 2 cut(s) 532, 641
SspMI CTAG 1 cut(s) 203
SstI GAGCTC 1 cut(s) 339
StyI CCWWGG 1 cut(s) 273
TaaI ACNGT 2 cut(s) 140, 360
TaiI ACGT 1 cut(s) 396
TaqI TCGA 2 cut(s) 85, 156
TasI AATT 4 cut(s) 102, 145, 191, 567
TfiI GAWTC 2 cut(s) 60, 470
Tru1I TTAA 1 cut(s) 643
Tru9I TTAA 1 cut(s) 643
TscAI CASTG 2 cut(s) 298, 363
TseFI GTSAC 1 cut(s) 289
TseI GCWGC 1 cut(s) 593
Tsp45I GTSAC 1 cut(s) 289
TspDTI ATGAA 3 cut(s) 249, 272, 435
TspRI CASTG 2 cut(s) 298, 363
VpaK11BI GGWCC 1 cut(s) 214
XceI RCATGY 1 cut(s) 35
XhoI CTCGAG 1 cut(s) 84
XspI CTAG 1 cut(s) 203
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.