Rw4G012300

Tropinone reductase homolog At1g07440-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0000208
Physical Location & Seq
Reverse (-)
28962006 .. 28963635
1630 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G012300.1

Sequence Viewer

Length: 765 bp
ATGGCTCAATATGGCGGAGGAAGAAGATGGTCTCTTGAAGGAATGACTGCACTCGTCACTGGTGGAACCAAAGGGATCGGGTATGCGATTGTTGAGGAATTGGCTGGATTAGGTGCAAGTGTACATACTTGCTCACGGAATGAAGCCCAGCTCAATGAATGCTTGAATCAATGGAAGAAGAAGGGTTTTCATCAAGTCACTGGTTCAGTGTGTGATGTGGTCTCAAAAATCCAGAGAGAGGAACTAATACAGAAGGTCTCATCACTGTTTCATGGGAAACTTAATATCCTTATAAACAACGTGGGAGCTCAAATAGGAAAGCCAGCAACTGAGTACACAGCTGAAGACTACTCATTTATCATGAGTACTAATCTTGAATCTACTTACACTATGTGCCAACTAGCACATCCTCTTCTCAAAACTTCAGGAGCTGGTAACATTATTTTTCTGTCTTCTGTTGGTGGTGTGGTCTCAATGGGGGAGGGTACTAGTGTATACGGTGCAACTAAAGGAGCAATGAATCAATTGGCCAAAACTTTGGCTTGTGAGTGGGCAAAAGACAAGATTAGGACTAATAGTGTTGCACCTGCGCTCATCAGAACTCCTCTTGTTGAACCTGTAAGATACATTCGACGTATAGGAGAGCCTGAAGAGGTGTCTGCCTTGGTGGCATTTCTATGCCTTCCTGCAGCCTCATACATAACAGGGCAGACTATTTGCATCGATGGAGGGCTAACAGTCAATGGTTTCCAGTTCCAAGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00960 ko01100 ko01110 map00960 map01100 map01110
Pfam Domains
Protein Families
adh_short_C2

Protein Analysis

254

Amino Acids

27.17

Weight (kDa)

8.13

Isoelectric Point (pI)

29.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short PF00106 16 - 207 4.5e-47 short chain dehydrogenase
KR PF08659 16 - 179 1.5e-09 KR domain
adh_short_C2 PF13561 24 - 246 8.6e-50 Enoyl-(Acyl carrier protein) reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000208)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G07440 AT1G07440 AT2G29150 AT2G29170 AT2G29290 AT2G29290 AT2G29300 AT2G29300 AT2G29310 AT2G29310 AT2G29310 AT2G29310 AT2G29320 AT2G29320 AT2G29320 AT2G29340 AT2G29340 AT2G29340 AT2G29340 AT2G29350 AT2G29350 AT2G29350
fragaria_vesca FvH4_3g01040 FvH4_3g36954 FvH4_3g36954 FvH4_3g36955 FvH4_3g36960 FvH4_3g36960 FvH4_3g37000 FvH4_3g37000 FvH4_3g37000
malus_domestica MD03G1085500.v1.1 MD03G1085700.v1.1 MD04G1134700.v1.1 MD11G1093400.v1.1 MD11G1093500.v1.1 MD11G1093700.v1.1 MD11G1093800.v1.1 MD11G1093900.v1.1 MD11G1094300.v1.1
prunus_persica Prupe.6G068900_v2.0.a1 Prupe.6G069000_v2.0.a1 Prupe.6G069000_v2.0.a1 Prupe.6G069000_v2.0.a1 Prupe.6G069100_v2.0.a1 Prupe.6G069200_v2.0.a1 Prupe.6G069500_v2.0.a1 Prupe.6G069500_v2.0.a1 Prupe.6G069700_v2.0.a1 Prupe.6G069900_v2.0.a1 Prupe.6G070000_v2.0.a1 Prupe.6G070000_v2.0.a1 Prupe.6G070000_v2.0.a1 Prupe.6G070000_v2.0.a1 Prupe.6G070000_v2.0.a1
pyrus_communis pycom03g06770 pycom11g07900 pycom11g07910 pycom11g07920 pycom11g07940 pycom11g07950 pycom11g07970 pycom11g07990 pycom12g14220
rosa_chinensis RchiOBHm_Chr2g0131701 RchiOBHm_Chr3g0473751 RchiOBHm_Chr4g0408441 RchiOBHm_Chr4g0408481 RchiOBHm_Chr4g0408501 RchiOBHm_Chr4g0408561 RchiOBHm_Chr4g0408611 RchiOBHm_Chr4g0408661 RchiOBHm_Chr4g0408721 RchiOBHm_Chr5g0045781 RchiOBHm_Chr5g0045851 RchiOBHm_Chr5g0068421 RchiOBHm_Chr7g0222951
rosa_laevigata RLG00000008645 RLG00000008646 RLG00000008649 RLG00000035864
rosa_multiflora Rmu_sc0000774.1_g000007 Rmu_sc0001746.1_g000006 Rmu_sc0002589.1_g000024 Rmu_sc0002736.1_g000004 Rmu_sc0003677.1_g000008 Rmu_sc0005087.1_g000003 Rmu_sc0005878.1_g000021 Rmu_sc0006514.1_g000011 Rmu_sc0025386.1_g000001 Rmu_sc0032361.1_g000001
rosa_roxburghii Rroxscaffold_1G00012360 Rroxscaffold_1G00013090 Rroxscaffold_1G00035120 Rroxscaffold_3G00236380 Rroxscaffold_5G00352920 Rroxscaffold_5G00352950 Rroxscaffold_5G00352980 Rroxscaffold_5G00353000 Rroxscaffold_5G00353020
rosa_rugosa Rorug02G0295300 Rorug04G0086300 Rorug04G0094800 Rorug05G0226700 Rorug05G0378300 Rorug05G0389700 Rorug07G0212400 Rorug07G0219800 Rorug07G0219900
rosa_samantha Rh1AG094900 Rh2AG351700 Rh2BG358700 Rh2CG337200 Rh2DG376100 Rh3DG297400 Rh3DG365100 Rh4AG150600 Rh4AG150800 Rh4BG147900 Rh4BG148000 Rh4BG148100 Rh4CG158000 Rh4CG158700 Rh4CG159300 Rh4DG143600 Rh4DG143800 Rh4DG144200 Rh5AG307900 Rh5AG448500 Rh5BG316300 Rh5BG316700 Rh5BG466500 Rh5CG342400 Rh5CG488100 Rh5DG326600 Rh5DG480000 Rh7AG357400 Rh7AG406600 Rh7BG345700 Rh7CG375600 Rh7DG352300
rosa_wichuraiana Rw2G028560 Rw4G012300 Rw4G012330 Rw4G012340 Rw4G012350 Rw5G022400 Rw5G028830 Rw5G041870 Rw7G030400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 293
AarI CACCTGC 1 cut(s) 595
Acc36I ACCTGC 1 cut(s) 595
AccI GTMKAC 1 cut(s) 495
AciI CCGC 1 cut(s) 15
AclWI GGATC 1 cut(s) 83
AcoI YGGCCR 1 cut(s) 528
AcuI CTGAAG 3 cut(s) 363, 408, 669
AdeI CACNNNGTG 1 cut(s) 393
AfaI GTAC 4 cut(s) 123, 335, 367, 487
AfiI CCNNNNNNNGG 1 cut(s) 238
AgsI TTSAA 4 cut(s) 38, 166, 377, 614
AhlI ACTAGT 1 cut(s) 488
AluBI AGCT 4 cut(s) 151, 308, 341, 431
AluI AGCT 4 cut(s) 151, 308, 341, 431
Alw21I GWGCWC 1 cut(s) 310
Alw26I GTCTC 4 cut(s) 36, 226, 262, 475
AlwI GGATC 1 cut(s) 83
AlwNI CAGNNNCTG 2 cut(s) 329, 431
AoxI GGCC 1 cut(s) 528
ApeKI GCWGC 1 cut(s) 689
AspLEI GCGC 1 cut(s) 592
BaeI ACNNNNGTAYC 2 cut(s) 477, 510
BalI TGGCCA 1 cut(s) 530
BanII GRGCYC 1 cut(s) 310
BbsI GAAGAC 2 cut(s) 351, 444
Bbv12I GWGCWC 1 cut(s) 310
BbvI GCAGC 1 cut(s) 701
BccI CCATC 2 cut(s) 21, 719
BcoDI GTCTC 4 cut(s) 36, 226, 262, 475
BcuI ACTAGT 1 cut(s) 488
BfaI CTAG 2 cut(s) 401, 489
BfmI CTRYAG 1 cut(s) 687
BfuAI ACCTGC 1 cut(s) 595
BglI GCCNNNNNGGC 1 cut(s) 668
BisI GCNGC 1 cut(s) 690
BlsI GCNGC 1 cut(s) 691
BmcAI AGTACT 1 cut(s) 367
BmiI GGNNCC 1 cut(s) 67
BmsI GCATC 1 cut(s) 729
BpiI GAAGAC 2 cut(s) 351, 444
Bsa29I ATCGAT 1 cut(s) 723
BsaI GGTCTC 4 cut(s) 36, 226, 262, 475
BsaJI CCNNGG 1 cut(s) 663
Bsc4I CCNNNNNNNGG 1 cut(s) 238
Bse1I ACTGG 3 cut(s) 64, 205, 751
Bse3DI GCAATG 1 cut(s) 522
BseCI ATCGAT 1 cut(s) 723
BseDI CCNNGG 1 cut(s) 663
BseGI GGATG 1 cut(s) 406
BseLI CCNNNNNNNGG 1 cut(s) 238
BseMI GCAATG 1 cut(s) 522
BseMII CTCAG 1 cut(s) 321
BseNI ACTGG 3 cut(s) 64, 205, 751
BseRI GAGGAG 1 cut(s) 594
BseXI GCAGC 1 cut(s) 701
BseYI CCCAGC 1 cut(s) 147
BsgI GTGCAG 1 cut(s) 33
BshFI GGCC 1 cut(s) 530
BshVI ATCGAT 1 cut(s) 723
BsiHKAI GWGCWC 1 cut(s) 310
BslI CCNNNNNNNGG 1 cut(s) 238
BsmAI GTCTC 4 cut(s) 36, 226, 262, 475
BsmI GAATGC 1 cut(s) 164
BsnI GGCC 1 cut(s) 530
Bso31I GGTCTC 4 cut(s) 36, 226, 262, 475
Bsp1286I GDGCHC 1 cut(s) 310
Bsp1407I TGTACA 1 cut(s) 121
Bsp143I GATC 1 cut(s) 75
BspACI CCGC 1 cut(s) 15
BspANI GGCC 1 cut(s) 530
BspCNI CTCAG 1 cut(s) 322
BspDI ATCGAT 1 cut(s) 723
BspHI TCATGA 1 cut(s) 360
BspLI GGNNCC 1 cut(s) 67
BspMAI CTGCAG 1 cut(s) 691
BspMI ACCTGC 1 cut(s) 595
BspPI GGATC 1 cut(s) 83
BspTNI GGTCTC 4 cut(s) 36, 226, 262, 475
BsrDI GCAATG 1 cut(s) 522
BsrGI TGTACA 1 cut(s) 121
BsrI ACTGG 3 cut(s) 64, 205, 751
BssECI CCNNGG 1 cut(s) 663
BssMI GATC 1 cut(s) 75
BssNAI GTATAC 1 cut(s) 496
BssT1I CCWWGG 1 cut(s) 663
Bst1107I GTATAC 1 cut(s) 496
Bst4CI ACNGT 3 cut(s) 267, 500, 739
Bst6I CTCTTC 2 cut(s) 417, 645
BstAUI TGTACA 1 cut(s) 121
BstC8I GCNNGC 1 cut(s) 324
BstDEI CTNAG 1 cut(s) 330
BstF5I GGATG 1 cut(s) 406
BstHHI GCGC 1 cut(s) 592
BstKTI GATC 1 cut(s) 78
BstMAI GTCTC 4 cut(s) 36, 226, 262, 475
BstMBI GATC 1 cut(s) 75
BstMWI GCNNNNNNNGC 1 cut(s) 668
BstSFI CTRYAG 1 cut(s) 687
BstV1I GCAGC 1 cut(s) 701
BstV2I GAAGAC 2 cut(s) 351, 444
BstXI CCANNNNNNTGG 1 cut(s) 538
BstZ17I GTATAC 1 cut(s) 496
Bsu15I ATCGAT 1 cut(s) 723
BsuRI GGCC 1 cut(s) 530
BsuTUI ATCGAT 1 cut(s) 723
BtsCI GGATG 1 cut(s) 406
BtsIMutI CAGTG 4 cut(s) 57, 198, 213, 263
BveI ACCTGC 1 cut(s) 595
Cac8I GCNNGC 1 cut(s) 324
CaiI CAGNNNCTG 2 cut(s) 329, 431
CciI TCATGA 1 cut(s) 360
CfoI GCGC 1 cut(s) 592
ClaI ATCGAT 1 cut(s) 723
Csp6I GTAC 4 cut(s) 122, 334, 366, 486
CviAII CATG 2 cut(s) 272, 361
CviQI GTAC 4 cut(s) 122, 334, 366, 486
DdeI CTNAG 1 cut(s) 330
DpnI GATC 1 cut(s) 77
DpnII GATC 1 cut(s) 75
DraIII CACNNNGTG 1 cut(s) 393
EaeI YGGCCR 1 cut(s) 528
Eam1104I CTCTTC 2 cut(s) 417, 645
EarI CTCTTC 2 cut(s) 417, 645
EciI GGCGGA 1 cut(s) 30
Ecl136II GAGCTC 1 cut(s) 308
Eco130I CCWWGG 1 cut(s) 663
Eco24I GRGCYC 1 cut(s) 310
Eco31I GGTCTC 4 cut(s) 36, 226, 262, 475
Eco53kI GAGCTC 1 cut(s) 308
Eco57I CTGAAG 3 cut(s) 363, 408, 669
EcoICRI GAGCTC 1 cut(s) 308
EcoT14I CCWWGG 1 cut(s) 663
EcoT38I GRGCYC 1 cut(s) 310
ErhI CCWWGG 1 cut(s) 663
FaeI CATG 2 cut(s) 275, 364
FatI CATG 2 cut(s) 271, 360
FblI GTMKAC 1 cut(s) 495
Fnu4HI GCNGC 1 cut(s) 690
FokI GGATG 1 cut(s) 393
FriOI GRGCYC 1 cut(s) 310
Fsp4HI GCNGC 1 cut(s) 690
FspBI CTAG 2 cut(s) 401, 489
GlaI GCGC 1 cut(s) 591
GluI GCNGC 1 cut(s) 690
GsaI CCCAGC 1 cut(s) 151
HaeIII GGCC 1 cut(s) 530
HhaI GCGC 1 cut(s) 592
Hin1II CATG 2 cut(s) 275, 364
Hin6I GCGC 1 cut(s) 590
HinP1I GCGC 1 cut(s) 590
HinfI GANTC 3 cut(s) 166, 377, 520
Hpy166II GTNNAC 3 cut(s) 122, 336, 496
Hpy188I TCNGA 1 cut(s) 599
Hpy188III TCNNGA 5 cut(s) 35, 232, 361, 374, 426
Hpy8I GTNNAC 3 cut(s) 122, 336, 496
Hpy99I CGWCG 1 cut(s) 636
HpyAV CCTTC 4 cut(s) 32, 175, 247, 692
HpyCH4III ACNGT 3 cut(s) 267, 500, 739
HpyCH4IV ACGT 2 cut(s) 300, 634
HpyCH4V TGCA 6 cut(s) 50, 116, 503, 584, 689, 720
HpyF10VI GCNNNNNNNGC 1 cut(s) 668
HpyF3I CTNAG 1 cut(s) 330
HpySE526I ACGT 2 cut(s) 300, 634
Hsp92II CATG 2 cut(s) 275, 364
HspAI GCGC 1 cut(s) 590
Kzo9I GATC 1 cut(s) 75
LmnI GCTCC 3 cut(s) 305, 428, 512
Lsp1109I GCAGC 1 cut(s) 701
LweI GCATC 1 cut(s) 729
MaeI CTAG 2 cut(s) 401, 489
MaeII ACGT 2 cut(s) 300, 634
MaeIII GTNAC 3 cut(s) 55, 196, 434
MalI GATC 1 cut(s) 77
MboI GATC 1 cut(s) 75
MboII GAAGA 8 cut(s) 33, 36, 187, 190, 356, 404, 444, 662
MfeI CAATTG 1 cut(s) 524
MhlI GDGCHC 1 cut(s) 310
MlsI TGGCCA 1 cut(s) 530
MluCI AATT 2 cut(s) 98, 524
MluNI TGGCCA 1 cut(s) 530
MnlI CCTC 9 cut(s) 11, 88, 232, 420, 475, 615, 646, 703, 722
Mox20I TGGCCA 1 cut(s) 530
MscI TGGCCA 1 cut(s) 530
MseI TTAA 1 cut(s) 282
MslI CAYNNNNRTG 1 cut(s) 676
Msp20I TGGCCA 1 cut(s) 530
MspA1I CMGCKG 1 cut(s) 341
MunI CAATTG 1 cut(s) 524
Mva1269I GAATGC 1 cut(s) 164
MwoI GCNNNNNNNGC 1 cut(s) 668
NdeII GATC 1 cut(s) 75
NlaIII CATG 2 cut(s) 275, 364
NlaIV GGNNCC 1 cut(s) 67
NmuCI GTSAC 2 cut(s) 55, 196
PagI TCATGA 1 cut(s) 360
PaqCI CACCTGC 1 cut(s) 595
PctI GAATGC 1 cut(s) 164
PfeI GAWTC 3 cut(s) 166, 377, 520
PkrI GCNGC 1 cut(s) 691
PsiI TTATAA 1 cut(s) 293
Psp124BI GAGCTC 1 cut(s) 310
PspFI CCCAGC 1 cut(s) 147
PspN4I GGNNCC 1 cut(s) 67
PstI CTGCAG 1 cut(s) 691
PstNI CAGNNNCTG 2 cut(s) 329, 431
PvuII CAGCTG 1 cut(s) 341
RsaI GTAC 4 cut(s) 123, 335, 367, 487
RsaNI GTAC 4 cut(s) 122, 334, 366, 486
RseI CAYNNNNRTG 1 cut(s) 676
SacI GAGCTC 1 cut(s) 310
SaqAI TTAA 1 cut(s) 282
SatI GCNGC 1 cut(s) 690
Sau3AI GATC 1 cut(s) 75
ScaI AGTACT 1 cut(s) 367
SduI GDGCHC 1 cut(s) 310
SfaNI GCATC 1 cut(s) 729
SfcI CTRYAG 1 cut(s) 687
SmiMI CAYNNNNRTG 1 cut(s) 676
SpeI ACTAGT 1 cut(s) 488
Sse9I AATT 2 cut(s) 98, 524
SsiI CCGC 1 cut(s) 15
SspMI CTAG 2 cut(s) 401, 489
SstI GAGCTC 1 cut(s) 310
StyI CCWWGG 1 cut(s) 663
TaaI ACNGT 3 cut(s) 267, 500, 739
TaiI ACGT 2 cut(s) 303, 637
TaqI TCGA 2 cut(s) 631, 723
TasI AATT 2 cut(s) 98, 524
TatI WGTACW 3 cut(s) 121, 333, 365
TfiI GAWTC 3 cut(s) 166, 377, 520
Tru1I TTAA 1 cut(s) 282
Tru9I TTAA 1 cut(s) 282
TscAI CASTG 4 cut(s) 64, 205, 213, 270
TseFI GTSAC 2 cut(s) 55, 196
TseI GCWGC 1 cut(s) 689
Tsp45I GTSAC 2 cut(s) 55, 196
TspDTI ATGAA 5 cut(s) 156, 171, 179, 260, 533
TspGWI ACGGA 1 cut(s) 151
TspRI CASTG 4 cut(s) 64, 205, 213, 270
XmiI GTMKAC 1 cut(s) 495
XspI CTAG 2 cut(s) 401, 489
ZrmI AGTACT 1 cut(s) 367
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.