Rh4BG148100

Tropinone reductase homolog At1g07440-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0000208
Physical Location & Seq
Reverse (-)
26516200 .. 26517737
1538 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG148100.1

Sequence Viewer

Length: 891 bp
ATGTATCTTCAAATTTTGGCCATCGAGATAGAAAAGAAAGAAACAGAGAGGGCTAGGCAGATGGCTGAAGCAAATAATCCTATTAGCAATGACAGCTTAGATAGAAGATGGTCTCTTGAAGGAATGACCGCTCTTGTCACGGGTGGAACCAAAGGCATTGGATATGCTGTTGTGGAGGAATTGGCGAGGCTAGGTGCAAAGGTCCATACTTGTGCTCGGAATGAAGTCCAACTCAATGAGCGGTTGAGTCAATGGAAGACTAAGGGTTTTCATCAAGTTACTGGTTCTGTATGTGATTTGGTCTCAAAGCCCCAACGAGAAGAGCTCATAAACAAGGTCTCATCACTGTTCAATGGAAAACTAAACATCCTTATAAACAACGTGGGAACCAATATATTAAAGCCCACAACTGATTACACAGCTGAAGATTATTCATTTCTTATGGGAACCAATCTTGAATCTTATTACAATATGTGCCAACTTGCACATCCTCTCCTCAAAGCTTCAGGAGCTGGTAATATTGTTCTTATATCTTCTATTGCTGGTGTGATGTCAATTGGGGGTAGTGGAAGTGTCTATGCTGCCACTAAAGGAGCAATTAACCAATTGGCAAGATACTTGGCTTGCGAGTGGGGAAAAGACAAGATTAGGATCAACAGTATTGCACCTGGGTTCATACGAACTCCCTTGGCTGAAGATATTCTCAATAACCAAATGATGCTTGAGGCTATTAAATCTGGAACACCTTTAGGACGCCATGGCCAGCCGGAAGAGGTTTCTTCCTTGGTGGCATTTCTATGCTTTCCTGCTGCCTCATACATAACTGGGCAGACTATCTGCATTGACGGAGGGATGACTGTTAATGGCATAAGCTTATGCTTTCAAGGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00960 ko01100 ko01110 map00960 map01100 map01110
Pfam Domains
Protein Families
adh_short adh_short_C2

Protein Analysis

296

Amino Acids

32.08

Weight (kDa)

6.9

Isoelectric Point (pI)

33.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SDR PF23441 41 - 285 2.5e-06 SDR-like rossmann domain
adh_short PF00106 43 - 235 1.5e-47 short chain dehydrogenase
KR PF08659 43 - 207 1.8e-09 KR domain
adh_short_C2 PF13561 51 - 286 1.4e-60 Enoyl-(Acyl carrier protein) reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000208)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G07440 AT1G07440 AT2G29150 AT2G29170 AT2G29290 AT2G29290 AT2G29300 AT2G29300 AT2G29310 AT2G29310 AT2G29310 AT2G29310 AT2G29320 AT2G29320 AT2G29320 AT2G29340 AT2G29340 AT2G29340 AT2G29340 AT2G29350 AT2G29350 AT2G29350
fragaria_vesca FvH4_3g01040 FvH4_3g36954 FvH4_3g36954 FvH4_3g36955 FvH4_3g36960 FvH4_3g36960 FvH4_3g37000 FvH4_3g37000 FvH4_3g37000
malus_domestica MD03G1085500.v1.1 MD03G1085700.v1.1 MD04G1134700.v1.1 MD11G1093400.v1.1 MD11G1093500.v1.1 MD11G1093700.v1.1 MD11G1093800.v1.1 MD11G1093900.v1.1 MD11G1094300.v1.1
prunus_persica Prupe.6G068900_v2.0.a1 Prupe.6G069000_v2.0.a1 Prupe.6G069000_v2.0.a1 Prupe.6G069000_v2.0.a1 Prupe.6G069100_v2.0.a1 Prupe.6G069200_v2.0.a1 Prupe.6G069500_v2.0.a1 Prupe.6G069500_v2.0.a1 Prupe.6G069700_v2.0.a1 Prupe.6G069900_v2.0.a1 Prupe.6G070000_v2.0.a1 Prupe.6G070000_v2.0.a1 Prupe.6G070000_v2.0.a1 Prupe.6G070000_v2.0.a1 Prupe.6G070000_v2.0.a1
pyrus_communis pycom03g06770 pycom11g07900 pycom11g07910 pycom11g07920 pycom11g07940 pycom11g07950 pycom11g07970 pycom11g07990 pycom12g14220
rosa_chinensis RchiOBHm_Chr2g0131701 RchiOBHm_Chr3g0473751 RchiOBHm_Chr4g0408441 RchiOBHm_Chr4g0408481 RchiOBHm_Chr4g0408501 RchiOBHm_Chr4g0408561 RchiOBHm_Chr4g0408611 RchiOBHm_Chr4g0408661 RchiOBHm_Chr4g0408721 RchiOBHm_Chr5g0045781 RchiOBHm_Chr5g0045851 RchiOBHm_Chr5g0068421 RchiOBHm_Chr7g0222951
rosa_laevigata RLG00000008645 RLG00000008646 RLG00000008649 RLG00000035864
rosa_multiflora Rmu_sc0000774.1_g000007 Rmu_sc0001746.1_g000006 Rmu_sc0002589.1_g000024 Rmu_sc0002736.1_g000004 Rmu_sc0003677.1_g000008 Rmu_sc0005087.1_g000003 Rmu_sc0005878.1_g000021 Rmu_sc0006514.1_g000011 Rmu_sc0025386.1_g000001 Rmu_sc0032361.1_g000001
rosa_roxburghii Rroxscaffold_1G00012360 Rroxscaffold_1G00013090 Rroxscaffold_1G00035120 Rroxscaffold_3G00236380 Rroxscaffold_5G00352920 Rroxscaffold_5G00352950 Rroxscaffold_5G00352980 Rroxscaffold_5G00353000 Rroxscaffold_5G00353020
rosa_rugosa Rorug02G0295300 Rorug04G0086300 Rorug04G0094800 Rorug05G0226700 Rorug05G0378300 Rorug05G0389700 Rorug07G0212400 Rorug07G0219800 Rorug07G0219900
rosa_samantha Rh1AG094900 Rh2AG351700 Rh2BG358700 Rh2CG337200 Rh2DG376100 Rh3DG297400 Rh3DG365100 Rh4AG150600 Rh4AG150800 Rh4BG147900 Rh4BG148000 Rh4BG148100 Rh4CG158000 Rh4CG158700 Rh4CG159300 Rh4DG143600 Rh4DG143800 Rh4DG144200 Rh5AG307900 Rh5AG448500 Rh5BG316300 Rh5BG316700 Rh5BG466500 Rh5CG342400 Rh5CG488100 Rh5DG326600 Rh5DG480000 Rh7AG357400 Rh7AG406600 Rh7BG345700 Rh7CG375600 Rh7DG352300
rosa_wichuraiana Rw2G028560 Rw4G012300 Rw4G012330 Rw4G012340 Rw4G012350 Rw5G022400 Rw5G028830 Rw5G041870 Rw7G030400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 374
AccBSI CCGCTC 2 cut(s) 131, 241
AciI CCGC 2 cut(s) 129, 241
AclWI GGATC 1 cut(s) 659
AcoI YGGCCR 2 cut(s) 18, 760
AcsI RAATTY 1 cut(s) 12
AcuI CTGAAG 4 cut(s) 87, 444, 489, 714
AcyI GRCGYC 1 cut(s) 754
AgsI TTSAA 5 cut(s) 11, 119, 352, 458, 884
AjnI CCWGG 1 cut(s) 667
AluBI AGCT 6 cut(s) 96, 325, 422, 503, 512, 873
AluI AGCT 6 cut(s) 96, 325, 422, 503, 512, 873
Alw21I GWGCWC 2 cut(s) 217, 327
Alw26I GTCTC 3 cut(s) 117, 307, 343
AlwI GGATC 1 cut(s) 659
AlwNI CAGNNNCTG 1 cut(s) 512
AoxI GGCC 2 cut(s) 18, 760
ApeKI GCWGC 2 cut(s) 581, 809
ApoI RAATTY 1 cut(s) 12
Asp700I GAANNNNTTC 1 cut(s) 699
AspS9I GGNCC 1 cut(s) 202
AvaII GGWCC 1 cut(s) 202
BalI TGGCCA 2 cut(s) 20, 762
BanII GRGCYC 1 cut(s) 327
BbsI GAAGAC 1 cut(s) 263
Bbv12I GWGCWC 2 cut(s) 217, 327
BbvI GCAGC 2 cut(s) 568, 796
BccI CCATC 3 cut(s) 29, 55, 102
BciT130I CCWGG 1 cut(s) 669
BcoDI GTCTC 3 cut(s) 117, 307, 343
BfaI CTAG 3 cut(s) 54, 191, 889
BisI GCNGC 2 cut(s) 582, 810
BlsI GCNGC 2 cut(s) 583, 811
Bme1390I CCNGG 1 cut(s) 669
Bme18I GGWCC 1 cut(s) 202
BmgT120I GGNCC 1 cut(s) 202
BmiI GGNNCC 3 cut(s) 148, 388, 448
BmrFI CCNGG 1 cut(s) 669
BmrI ACTGGG 1 cut(s) 834
BmsI GCATC 1 cut(s) 708
BmuI ACTGGG 1 cut(s) 834
BpiI GAAGAC 1 cut(s) 263
BplI GAGNNNNNCTC 2 cut(s) 309, 341
BpuEI CTTGAG 1 cut(s) 743
BsaBI GATNNNNATC 1 cut(s) 650
BsaHI GRCGYC 1 cut(s) 754
BsaI GGTCTC 3 cut(s) 117, 307, 343
BsaJI CCNNGG 4 cut(s) 668, 687, 757, 783
BsaXI ACNNNNNCTCC 2 cut(s) 477, 507
Bse1I ACTGG 2 cut(s) 286, 829
Bse3DI GCAATG 1 cut(s) 94
Bse8I GATNNNNATC 1 cut(s) 650
BseBI CCWGG 1 cut(s) 669
BseDI CCNNGG 4 cut(s) 668, 687, 757, 783
BseGI GGATG 3 cut(s) 366, 487, 858
BseJI GATNNNNATC 1 cut(s) 650
BseMI GCAATG 1 cut(s) 94
BseNI ACTGG 2 cut(s) 286, 829
BseRI GAGGAG 1 cut(s) 485
BseXI GCAGC 2 cut(s) 568, 796
BshFI GGCC 2 cut(s) 20, 762
BsiHKAI GWGCWC 2 cut(s) 217, 327
BsiSI CCGG 1 cut(s) 767
BsmAI GTCTC 3 cut(s) 117, 307, 343
BsnI GGCC 2 cut(s) 20, 762
Bso31I GGTCTC 3 cut(s) 117, 307, 343
Bsp1286I GDGCHC 2 cut(s) 217, 327
Bsp143I GATC 1 cut(s) 651
Bsp19I CCATGG 1 cut(s) 757
BspACI CCGC 2 cut(s) 129, 241
BspANI GGCC 2 cut(s) 20, 762
BspLI GGNNCC 3 cut(s) 148, 388, 448
BspPI GGATC 1 cut(s) 659
BspQI GCTCTTC 1 cut(s) 315
BspTNI GGTCTC 3 cut(s) 117, 307, 343
BsrBI CCGCTC 2 cut(s) 131, 241
BsrDI GCAATG 1 cut(s) 94
BsrI ACTGG 2 cut(s) 286, 829
BssECI CCNNGG 4 cut(s) 668, 687, 757, 783
BssMI GATC 1 cut(s) 651
BssNI GRCGYC 1 cut(s) 754
BssT1I CCWWGG 3 cut(s) 687, 757, 783
Bst2UI CCWGG 1 cut(s) 669
Bst4CI ACNGT 3 cut(s) 348, 659, 859
Bst6I CTCTTC 2 cut(s) 315, 765
BstACI GRCGYC 1 cut(s) 754
BstC8I GCNNGC 2 cut(s) 625, 764
BstDEI CTNAG 2 cut(s) 97, 261
BstDSI CCRYGG 1 cut(s) 757
BstF5I GGATG 3 cut(s) 366, 487, 858
BstKTI GATC 1 cut(s) 654
BstMAI GTCTC 3 cut(s) 117, 307, 343
BstMBI GATC 1 cut(s) 651
BstMWI GCNNNNNNNGC 3 cut(s) 93, 509, 885
BstNI CCWGG 1 cut(s) 669
BstSCI CCNGG 1 cut(s) 667
BstV1I GCAGC 2 cut(s) 568, 796
BstV2I GAAGAC 1 cut(s) 263
BsuRI GGCC 2 cut(s) 20, 762
BtgI CCRYGG 1 cut(s) 757
BtsCI GGATG 3 cut(s) 366, 487, 858
BtsIMutI CAGTG 1 cut(s) 344
Cac8I GCNNGC 2 cut(s) 625, 764
CaiI CAGNNNCTG 1 cut(s) 512
Cfr13I GGNCC 1 cut(s) 202
CseI GACGC 1 cut(s) 762
CviAII CATG 1 cut(s) 758
DdeI CTNAG 2 cut(s) 97, 261
DpnI GATC 1 cut(s) 653
DpnII GATC 1 cut(s) 651
EaeI YGGCCR 2 cut(s) 18, 760
Eam1104I CTCTTC 2 cut(s) 315, 765
EarI CTCTTC 2 cut(s) 315, 765
Ecl136II GAGCTC 1 cut(s) 325
Eco130I CCWWGG 3 cut(s) 687, 757, 783
Eco24I GRGCYC 1 cut(s) 327
Eco31I GGTCTC 3 cut(s) 117, 307, 343
Eco47I GGWCC 1 cut(s) 202
Eco53kI GAGCTC 1 cut(s) 325
Eco57I CTGAAG 4 cut(s) 87, 444, 489, 714
EcoICRI GAGCTC 1 cut(s) 325
EcoRII CCWGG 1 cut(s) 667
EcoT14I CCWWGG 3 cut(s) 687, 757, 783
EcoT38I GRGCYC 1 cut(s) 327
ErhI CCWWGG 3 cut(s) 687, 757, 783
FaeI CATG 1 cut(s) 761
FatI CATG 1 cut(s) 757
Fnu4HI GCNGC 2 cut(s) 582, 810
FokI GGATG 3 cut(s) 353, 474, 865
FriOI GRGCYC 1 cut(s) 327
Fsp4HI GCNGC 2 cut(s) 582, 810
FspBI CTAG 3 cut(s) 54, 191, 889
GluI GCNGC 2 cut(s) 582, 810
HaeIII GGCC 2 cut(s) 20, 762
HapII CCGG 1 cut(s) 767
HgaI GACGC 1 cut(s) 762
Hin1I GRCGYC 1 cut(s) 754
Hin1II CATG 1 cut(s) 761
HindIII AAGCTT 2 cut(s) 501, 871
HinfI GANTC 2 cut(s) 247, 458
HpaII CCGG 1 cut(s) 767
Hpy188I TCNGA 1 cut(s) 219
Hpy188III TCNNGA 5 cut(s) 25, 116, 455, 507, 738
HpyAV CCTTC 1 cut(s) 113
HpyCH4III ACNGT 3 cut(s) 348, 659, 859
HpyCH4IV ACGT 1 cut(s) 381
HpyCH4V TGCA 4 cut(s) 197, 485, 665, 840
HpyF10VI GCNNNNNNNGC 3 cut(s) 93, 509, 885
HpyF3I CTNAG 2 cut(s) 97, 261
HpySE526I ACGT 1 cut(s) 381
Hsp92I GRCGYC 1 cut(s) 754
Hsp92II CATG 1 cut(s) 761
Kzo9I GATC 1 cut(s) 651
LguI GCTCTTC 1 cut(s) 315
LmnI GCTCC 2 cut(s) 509, 593
Lsp1109I GCAGC 2 cut(s) 568, 796
LweI GCATC 1 cut(s) 708
MaeI CTAG 3 cut(s) 54, 191, 889
MaeII ACGT 1 cut(s) 381
MaeIII GTNAC 2 cut(s) 136, 277
MalI GATC 1 cut(s) 653
MbiI CCGCTC 2 cut(s) 131, 241
MboI GATC 1 cut(s) 651
MboII GAAGA 8 cut(s) 117, 268, 332, 437, 525, 707, 771, 782
MfeI CAATTG 2 cut(s) 555, 605
MhlI GDGCHC 2 cut(s) 217, 327
MlsI TGGCCA 2 cut(s) 20, 762
MluCI AATT 5 cut(s) 12, 179, 555, 597, 605
MluNI TGGCCA 2 cut(s) 20, 762
MlyI GAGTC 1 cut(s) 256
MmeI TCCRAC 1 cut(s) 253
MnlI CCTC 9 cut(s) 42, 169, 180, 501, 506, 718, 766, 823, 842
Mox20I TGGCCA 2 cut(s) 20, 762
MroXI GAANNNNTTC 1 cut(s) 699
MscI TGGCCA 2 cut(s) 20, 762
MseI TTAA 4 cut(s) 398, 600, 732, 861
MslI CAYNNNNRTG 2 cut(s) 210, 796
Msp20I TGGCCA 2 cut(s) 20, 762
MspA1I CMGCKG 1 cut(s) 422
MspI CCGG 1 cut(s) 767
MspR9I CCNGG 1 cut(s) 669
MunI CAATTG 2 cut(s) 555, 605
MvaI CCWGG 1 cut(s) 669
MwoI GCNNNNNNNGC 3 cut(s) 93, 509, 885
NcoI CCATGG 1 cut(s) 757
NdeII GATC 1 cut(s) 651
NlaIII CATG 1 cut(s) 761
NlaIV GGNNCC 3 cut(s) 148, 388, 448
NmuCI GTSAC 1 cut(s) 136
PciSI GCTCTTC 1 cut(s) 315
PdmI GAANNNNTTC 1 cut(s) 699
PfeI GAWTC 1 cut(s) 458
PkrI GCNGC 2 cut(s) 583, 811
PleI GAGTC 1 cut(s) 255
PpsI GAGTC 1 cut(s) 255
PsiI TTATAA 1 cut(s) 374
Psp124BI GAGCTC 1 cut(s) 327
Psp6I CCWGG 1 cut(s) 667
PspGI CCWGG 1 cut(s) 667
PspN4I GGNNCC 3 cut(s) 148, 388, 448
PspPI GGNCC 1 cut(s) 202
PstNI CAGNNNCTG 1 cut(s) 512
PvuII CAGCTG 1 cut(s) 422
RseI CAYNNNNRTG 2 cut(s) 210, 796
SacI GAGCTC 1 cut(s) 327
SapI GCTCTTC 1 cut(s) 315
SaqAI TTAA 4 cut(s) 398, 600, 732, 861
SatI GCNGC 2 cut(s) 582, 810
Sau3AI GATC 1 cut(s) 651
Sau96I GGNCC 1 cut(s) 202
SchI GAGTC 1 cut(s) 256
ScrFI CCNGG 1 cut(s) 669
SduI GDGCHC 2 cut(s) 217, 327
SfaNI GCATC 1 cut(s) 708
SinI GGWCC 1 cut(s) 202
SmiMI CAYNNNNRTG 2 cut(s) 210, 796
SmlI CTYRAG 1 cut(s) 722
SmoI CTYRAG 1 cut(s) 722
Sse9I AATT 5 cut(s) 12, 179, 555, 597, 605
SsiI CCGC 2 cut(s) 129, 241
SspI AATATT 1 cut(s) 520
SspMI CTAG 3 cut(s) 54, 191, 889
SstI GAGCTC 1 cut(s) 327
StyD4I CCNGG 1 cut(s) 667
StyI CCWWGG 3 cut(s) 687, 757, 783
TaaI ACNGT 3 cut(s) 348, 659, 859
TaiI ACGT 1 cut(s) 384
TaqI TCGA 1 cut(s) 24
TasI AATT 5 cut(s) 12, 179, 555, 597, 605
TfiI GAWTC 1 cut(s) 458
Tru1I TTAA 4 cut(s) 398, 600, 732, 861
Tru9I TTAA 4 cut(s) 398, 600, 732, 861
TscAI CASTG 1 cut(s) 351
TseFI GTSAC 1 cut(s) 136
TseI GCWGC 2 cut(s) 581, 809
Tsp45I GTSAC 1 cut(s) 136
TspDTI ATGAA 4 cut(s) 237, 260, 423, 664
TspGWI ACGGA 1 cut(s) 861
TspRI CASTG 1 cut(s) 351
VpaK11BI GGWCC 1 cut(s) 202
XapI RAATTY 1 cut(s) 12
XmnI GAANNNNTTC 1 cut(s) 699
XspI CTAG 3 cut(s) 54, 191, 889
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.