Rroxscaffold_5G00352920

Tropinone reductase homolog At1g07440-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0000208
Physical Location & Seq
Reverse (-)
30328967 .. 30330907
1941 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00352920.1

Sequence Viewer

Length: 762 bp
ATGACTGCTCTCGTCACTGGTGGAACCAAAGGGATCGGGTATGCGATTGTTGAGGAATTGGCTGGATTAGGTGCAAGTGTACATACTTGCTCACGGAATGAAGCCCAGCTCAATGAATGCTTGAATCAATGGAAGAAGAAGGGTTTTCATCAAGTCACTGGTTCAGTGTGTGATGTGGTCTCAAAAATCCAGAGAGAGGAACTAATACAGAAGGTCTCATCACTGTTTCATGGGAAACTTAATATCCTTATAAACAACGTGGGAGCTCAAATAGGAAAGCCAGCAACTGAGTACACAGCTGAAGACTACTCATTTATCATGAGTACTAATCTTGAATCTACTTACACTATGTGCCAACTAGCACATCCTCTTCTCAAAACTTCAGGAGCTGGTAACATTATTTTTCTGTCTTCTATTGGTGGTGTGGTCTCAATGGGGGAGGGTACGAGTGTATACGGTGCAACTAAAGGAGCAATGAATCAATTGGCCAAAACTTTGGCTTGTGAATGGGCAAAAGACAAGATTAGGATTAATAGTGTTGCACCTGCGCTCATCAGAACTCCTCTTGTTGAACCTATTTTGAATGAGGAAAAGTTTATGAAGGCTATGATCTCTCGAACCCCATTAGGACGTATTGGAGAGCCTGAAGAGGTGTCTGCCTTGGTGGCATTTCTATGCCTTCCTGCAGCCTCATATATAACAGGGCAGACTATTTGCATCGATGGAGGGCTAACAGTCAATGGTTTCCAGTTCCAAGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00960 ko01100 ko01110 map00960 map01100 map01110
Pfam Domains
Protein Families
adh_short_C2

Protein Analysis

253

Amino Acids

27.02

Weight (kDa)

6.89

Isoelectric Point (pI)

25.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short PF00106 2 - 196 1.1e-47 short chain dehydrogenase
KR PF08659 2 - 165 1.2e-09 KR domain
adh_short_C2 PF13561 10 - 245 7.1e-60 Enoyl-(Acyl carrier protein) reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000208)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G07440 AT1G07440 AT2G29150 AT2G29170 AT2G29290 AT2G29290 AT2G29300 AT2G29300 AT2G29310 AT2G29310 AT2G29310 AT2G29310 AT2G29320 AT2G29320 AT2G29320 AT2G29340 AT2G29340 AT2G29340 AT2G29340 AT2G29350 AT2G29350 AT2G29350
fragaria_vesca FvH4_3g01040 FvH4_3g36954 FvH4_3g36954 FvH4_3g36955 FvH4_3g36960 FvH4_3g36960 FvH4_3g37000 FvH4_3g37000 FvH4_3g37000
malus_domestica MD03G1085500.v1.1 MD03G1085700.v1.1 MD04G1134700.v1.1 MD11G1093400.v1.1 MD11G1093500.v1.1 MD11G1093700.v1.1 MD11G1093800.v1.1 MD11G1093900.v1.1 MD11G1094300.v1.1
prunus_persica Prupe.6G068900_v2.0.a1 Prupe.6G069000_v2.0.a1 Prupe.6G069000_v2.0.a1 Prupe.6G069000_v2.0.a1 Prupe.6G069100_v2.0.a1 Prupe.6G069200_v2.0.a1 Prupe.6G069500_v2.0.a1 Prupe.6G069500_v2.0.a1 Prupe.6G069700_v2.0.a1 Prupe.6G069900_v2.0.a1 Prupe.6G070000_v2.0.a1 Prupe.6G070000_v2.0.a1 Prupe.6G070000_v2.0.a1 Prupe.6G070000_v2.0.a1 Prupe.6G070000_v2.0.a1
pyrus_communis pycom03g06770 pycom11g07900 pycom11g07910 pycom11g07920 pycom11g07940 pycom11g07950 pycom11g07970 pycom11g07990 pycom12g14220
rosa_chinensis RchiOBHm_Chr2g0131701 RchiOBHm_Chr3g0473751 RchiOBHm_Chr4g0408441 RchiOBHm_Chr4g0408481 RchiOBHm_Chr4g0408501 RchiOBHm_Chr4g0408561 RchiOBHm_Chr4g0408611 RchiOBHm_Chr4g0408661 RchiOBHm_Chr4g0408721 RchiOBHm_Chr5g0045781 RchiOBHm_Chr5g0045851 RchiOBHm_Chr5g0068421 RchiOBHm_Chr7g0222951
rosa_laevigata RLG00000008645 RLG00000008646 RLG00000008649 RLG00000035864
rosa_multiflora Rmu_sc0000774.1_g000007 Rmu_sc0001746.1_g000006 Rmu_sc0002589.1_g000024 Rmu_sc0002736.1_g000004 Rmu_sc0003677.1_g000008 Rmu_sc0005087.1_g000003 Rmu_sc0005878.1_g000021 Rmu_sc0006514.1_g000011 Rmu_sc0025386.1_g000001 Rmu_sc0032361.1_g000001
rosa_roxburghii Rroxscaffold_1G00012360 Rroxscaffold_1G00013090 Rroxscaffold_1G00035120 Rroxscaffold_3G00236380 Rroxscaffold_5G00352920 Rroxscaffold_5G00352950 Rroxscaffold_5G00352980 Rroxscaffold_5G00353000 Rroxscaffold_5G00353020
rosa_rugosa Rorug02G0295300 Rorug04G0086300 Rorug04G0094800 Rorug05G0226700 Rorug05G0378300 Rorug05G0389700 Rorug07G0212400 Rorug07G0219800 Rorug07G0219900
rosa_samantha Rh1AG094900 Rh2AG351700 Rh2BG358700 Rh2CG337200 Rh2DG376100 Rh3DG297400 Rh3DG365100 Rh4AG150600 Rh4AG150800 Rh4BG147900 Rh4BG148000 Rh4BG148100 Rh4CG158000 Rh4CG158700 Rh4CG159300 Rh4DG143600 Rh4DG143800 Rh4DG144200 Rh5AG307900 Rh5AG448500 Rh5BG316300 Rh5BG316700 Rh5BG466500 Rh5CG342400 Rh5CG488100 Rh5DG326600 Rh5DG480000 Rh7AG357400 Rh7AG406600 Rh7BG345700 Rh7CG375600 Rh7DG352300
rosa_wichuraiana Rw2G028560 Rw4G012300 Rw4G012330 Rw4G012340 Rw4G012350 Rw5G022400 Rw5G028830 Rw5G041870 Rw7G030400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 251
AarI CACCTGC 1 cut(s) 553
Acc36I ACCTGC 1 cut(s) 553
AccI GTMKAC 1 cut(s) 453
AclWI GGATC 1 cut(s) 41
AcoI YGGCCR 1 cut(s) 486
AcuI CTGAAG 3 cut(s) 321, 366, 666
AdeI CACNNNGTG 1 cut(s) 351
AfaI GTAC 4 cut(s) 81, 293, 325, 445
AfiI CCNNNNNNNGG 1 cut(s) 196
AgsI TTSAA 4 cut(s) 124, 335, 572, 583
AluBI AGCT 4 cut(s) 109, 266, 299, 389
AluI AGCT 4 cut(s) 109, 266, 299, 389
Alw21I GWGCWC 1 cut(s) 268
Alw26I GTCTC 3 cut(s) 184, 220, 433
AlwI GGATC 1 cut(s) 41
AlwNI CAGNNNCTG 2 cut(s) 287, 389
AoxI GGCC 1 cut(s) 486
ApeKI GCWGC 1 cut(s) 686
AseI ATTAAT 1 cut(s) 531
AspLEI GCGC 1 cut(s) 550
BaeI ACNNNNGTAYC 2 cut(s) 435, 468
BalI TGGCCA 1 cut(s) 488
BanII GRGCYC 1 cut(s) 268
BbsI GAAGAC 2 cut(s) 309, 402
Bbv12I GWGCWC 1 cut(s) 268
BbvI GCAGC 1 cut(s) 698
BccI CCATC 1 cut(s) 716
BcoDI GTCTC 3 cut(s) 184, 220, 433
BfaI CTAG 1 cut(s) 359
BfmI CTRYAG 1 cut(s) 684
BfuAI ACCTGC 1 cut(s) 553
BglI GCCNNNNNGGC 1 cut(s) 665
BisI GCNGC 1 cut(s) 687
BlsI GCNGC 1 cut(s) 688
BmcAI AGTACT 1 cut(s) 325
BmiI GGNNCC 1 cut(s) 25
BmsI GCATC 1 cut(s) 726
BpiI GAAGAC 2 cut(s) 309, 402
Bsa29I ATCGAT 1 cut(s) 720
BsaI GGTCTC 3 cut(s) 184, 220, 433
BsaJI CCNNGG 1 cut(s) 660
Bsc4I CCNNNNNNNGG 1 cut(s) 196
Bse1I ACTGG 3 cut(s) 22, 163, 748
Bse3DI GCAATG 1 cut(s) 480
BseCI ATCGAT 1 cut(s) 720
BseDI CCNNGG 1 cut(s) 660
BseGI GGATG 1 cut(s) 364
BseLI CCNNNNNNNGG 1 cut(s) 196
BseMI GCAATG 1 cut(s) 480
BseMII CTCAG 1 cut(s) 279
BseNI ACTGG 3 cut(s) 22, 163, 748
BseRI GAGGAG 1 cut(s) 552
BseXI GCAGC 1 cut(s) 698
BseYI CCCAGC 1 cut(s) 105
BshFI GGCC 1 cut(s) 488
BshVI ATCGAT 1 cut(s) 720
BsiHKAI GWGCWC 1 cut(s) 268
BslI CCNNNNNNNGG 1 cut(s) 196
BsmAI GTCTC 3 cut(s) 184, 220, 433
BsmI GAATGC 1 cut(s) 122
BsnI GGCC 1 cut(s) 488
Bso31I GGTCTC 3 cut(s) 184, 220, 433
Bsp1286I GDGCHC 1 cut(s) 268
Bsp1407I TGTACA 1 cut(s) 79
Bsp143I GATC 2 cut(s) 33, 609
BspANI GGCC 1 cut(s) 488
BspCNI CTCAG 1 cut(s) 280
BspDI ATCGAT 1 cut(s) 720
BspHI TCATGA 1 cut(s) 318
BspLI GGNNCC 1 cut(s) 25
BspMAI CTGCAG 1 cut(s) 688
BspMI ACCTGC 1 cut(s) 553
BspPI GGATC 1 cut(s) 41
BspTNI GGTCTC 3 cut(s) 184, 220, 433
BsrDI GCAATG 1 cut(s) 480
BsrGI TGTACA 1 cut(s) 79
BsrI ACTGG 3 cut(s) 22, 163, 748
BssECI CCNNGG 1 cut(s) 660
BssMI GATC 2 cut(s) 33, 609
BssNAI GTATAC 1 cut(s) 454
BssT1I CCWWGG 1 cut(s) 660
Bst1107I GTATAC 1 cut(s) 454
Bst4CI ACNGT 3 cut(s) 225, 458, 736
Bst6I CTCTTC 2 cut(s) 375, 642
BstAUI TGTACA 1 cut(s) 79
BstC8I GCNNGC 1 cut(s) 282
BstDEI CTNAG 1 cut(s) 288
BstF5I GGATG 1 cut(s) 364
BstHHI GCGC 1 cut(s) 550
BstKTI GATC 2 cut(s) 36, 612
BstMAI GTCTC 3 cut(s) 184, 220, 433
BstMBI GATC 2 cut(s) 33, 609
BstMWI GCNNNNNNNGC 1 cut(s) 665
BstSFI CTRYAG 1 cut(s) 684
BstV1I GCAGC 1 cut(s) 698
BstV2I GAAGAC 2 cut(s) 309, 402
BstXI CCANNNNNNTGG 1 cut(s) 496
BstZ17I GTATAC 1 cut(s) 454
Bsu15I ATCGAT 1 cut(s) 720
BsuRI GGCC 1 cut(s) 488
BsuTUI ATCGAT 1 cut(s) 720
BtsCI GGATG 1 cut(s) 364
BtsIMutI CAGTG 4 cut(s) 15, 156, 171, 221
BveI ACCTGC 1 cut(s) 553
Cac8I GCNNGC 1 cut(s) 282
CaiI CAGNNNCTG 2 cut(s) 287, 389
CciI TCATGA 1 cut(s) 318
CfoI GCGC 1 cut(s) 550
ClaI ATCGAT 1 cut(s) 720
Csp6I GTAC 4 cut(s) 80, 292, 324, 444
CviAII CATG 2 cut(s) 230, 319
CviQI GTAC 4 cut(s) 80, 292, 324, 444
DdeI CTNAG 1 cut(s) 288
DpnI GATC 2 cut(s) 35, 611
DpnII GATC 2 cut(s) 33, 609
DraIII CACNNNGTG 1 cut(s) 351
EaeI YGGCCR 1 cut(s) 486
Eam1104I CTCTTC 2 cut(s) 375, 642
EarI CTCTTC 2 cut(s) 375, 642
Ecl136II GAGCTC 1 cut(s) 266
Eco130I CCWWGG 1 cut(s) 660
Eco24I GRGCYC 1 cut(s) 268
Eco31I GGTCTC 3 cut(s) 184, 220, 433
Eco53kI GAGCTC 1 cut(s) 266
Eco57I CTGAAG 3 cut(s) 321, 366, 666
EcoICRI GAGCTC 1 cut(s) 266
EcoT14I CCWWGG 1 cut(s) 660
EcoT38I GRGCYC 1 cut(s) 268
ErhI CCWWGG 1 cut(s) 660
FaeI CATG 2 cut(s) 233, 322
FatI CATG 2 cut(s) 229, 318
FblI GTMKAC 1 cut(s) 453
Fnu4HI GCNGC 1 cut(s) 687
FokI GGATG 1 cut(s) 351
FriOI GRGCYC 1 cut(s) 268
Fsp4HI GCNGC 1 cut(s) 687
FspBI CTAG 1 cut(s) 359
GlaI GCGC 1 cut(s) 549
GluI GCNGC 1 cut(s) 687
GsaI CCCAGC 1 cut(s) 109
HaeIII GGCC 1 cut(s) 488
HhaI GCGC 1 cut(s) 550
Hin1II CATG 2 cut(s) 233, 322
Hin6I GCGC 1 cut(s) 548
HinP1I GCGC 1 cut(s) 548
HinfI GANTC 3 cut(s) 124, 335, 478
Hpy166II GTNNAC 3 cut(s) 80, 294, 454
Hpy188I TCNGA 1 cut(s) 557
Hpy188III TCNNGA 5 cut(s) 190, 319, 332, 384, 615
Hpy8I GTNNAC 3 cut(s) 80, 294, 454
HpyAV CCTTC 4 cut(s) 133, 205, 595, 689
HpyCH4III ACNGT 3 cut(s) 225, 458, 736
HpyCH4IV ACGT 2 cut(s) 258, 631
HpyCH4V TGCA 5 cut(s) 74, 461, 542, 686, 717
HpyF10VI GCNNNNNNNGC 1 cut(s) 665
HpyF3I CTNAG 1 cut(s) 288
HpySE526I ACGT 2 cut(s) 258, 631
Hsp92II CATG 2 cut(s) 233, 322
HspAI GCGC 1 cut(s) 548
Kzo9I GATC 2 cut(s) 33, 609
LmnI GCTCC 3 cut(s) 263, 386, 470
Lsp1109I GCAGC 1 cut(s) 698
LweI GCATC 1 cut(s) 726
MaeI CTAG 1 cut(s) 359
MaeII ACGT 2 cut(s) 258, 631
MaeIII GTNAC 3 cut(s) 13, 154, 392
MalI GATC 2 cut(s) 35, 611
MboI GATC 2 cut(s) 33, 609
MboII GAAGA 6 cut(s) 145, 148, 314, 362, 402, 659
MfeI CAATTG 1 cut(s) 482
MhlI GDGCHC 1 cut(s) 268
MlsI TGGCCA 1 cut(s) 488
MluCI AATT 2 cut(s) 56, 482
MluNI TGGCCA 1 cut(s) 488
MnlI CCTC 9 cut(s) 46, 190, 378, 433, 573, 580, 643, 700, 719
Mox20I TGGCCA 1 cut(s) 488
MscI TGGCCA 1 cut(s) 488
MseI TTAA 2 cut(s) 240, 531
MslI CAYNNNNRTG 1 cut(s) 673
Msp20I TGGCCA 1 cut(s) 488
MspA1I CMGCKG 1 cut(s) 299
MunI CAATTG 1 cut(s) 482
Mva1269I GAATGC 1 cut(s) 122
MwoI GCNNNNNNNGC 1 cut(s) 665
NdeII GATC 2 cut(s) 33, 609
NlaIII CATG 2 cut(s) 233, 322
NlaIV GGNNCC 1 cut(s) 25
NmuCI GTSAC 2 cut(s) 13, 154
PagI TCATGA 1 cut(s) 318
PaqCI CACCTGC 1 cut(s) 553
PctI GAATGC 1 cut(s) 122
PfeI GAWTC 3 cut(s) 124, 335, 478
PkrI GCNGC 1 cut(s) 688
PshBI ATTAAT 1 cut(s) 531
PsiI TTATAA 1 cut(s) 251
Psp124BI GAGCTC 1 cut(s) 268
PspFI CCCAGC 1 cut(s) 105
PspN4I GGNNCC 1 cut(s) 25
PstI CTGCAG 1 cut(s) 688
PstNI CAGNNNCTG 2 cut(s) 287, 389
PvuII CAGCTG 1 cut(s) 299
RsaI GTAC 4 cut(s) 81, 293, 325, 445
RsaNI GTAC 4 cut(s) 80, 292, 324, 444
RseI CAYNNNNRTG 1 cut(s) 673
SacI GAGCTC 1 cut(s) 268
SaqAI TTAA 2 cut(s) 240, 531
SatI GCNGC 1 cut(s) 687
Sau3AI GATC 2 cut(s) 33, 609
ScaI AGTACT 1 cut(s) 325
SduI GDGCHC 1 cut(s) 268
SfaNI GCATC 1 cut(s) 726
SfcI CTRYAG 1 cut(s) 684
SmiMI CAYNNNNRTG 1 cut(s) 673
Sse9I AATT 2 cut(s) 56, 482
SspMI CTAG 1 cut(s) 359
SstI GAGCTC 1 cut(s) 268
StyI CCWWGG 1 cut(s) 660
TaaI ACNGT 3 cut(s) 225, 458, 736
TaiI ACGT 2 cut(s) 261, 634
TaqI TCGA 2 cut(s) 616, 720
TasI AATT 2 cut(s) 56, 482
TatI WGTACW 3 cut(s) 79, 291, 323
TfiI GAWTC 3 cut(s) 124, 335, 478
Tru1I TTAA 2 cut(s) 240, 531
Tru9I TTAA 2 cut(s) 240, 531
TscAI CASTG 4 cut(s) 22, 163, 171, 228
TseFI GTSAC 2 cut(s) 13, 154
TseI GCWGC 1 cut(s) 686
Tsp45I GTSAC 2 cut(s) 13, 154
TspDTI ATGAA 6 cut(s) 114, 129, 137, 218, 491, 614
TspGWI ACGGA 1 cut(s) 109
TspRI CASTG 4 cut(s) 22, 163, 171, 228
VspI ATTAAT 1 cut(s) 531
XmiI GTMKAC 1 cut(s) 453
XspI CTAG 1 cut(s) 359
ZrmI AGTACT 1 cut(s) 325
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.