Rh4CG159300

Tropinone reductase homolog At1g07440-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0000208
Physical Location & Seq
Reverse (-)
35010556 .. 35012397
1842 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG159300.1

Sequence Viewer

Length: 828 bp
ATGGCTCAAGCAAATAATCCTATTAGCAATGACAGCTCAGATAGAAGATGGTCTCTCGAGGGAATGACCGCTCTTGTCACGGGTGGAACCAAAGGCATCGGATATGCTATTGTGGAGGAATTGGCGAGGCTAGGTGCAAAGGTCCATACTTGTGCTCGGAATGAAGTCCAACTCAATGAGCGCTTGAGTCAATGGAAGACCAAGGGTTTTCATCAAGTCACTGGTTCTGTATGTGATTTGATCTCAAAGCCCCAACGAGAAGAGCTCATAAACAAGGTCTCATCACTGTTCAATGGAAAACTAAACATCCTTATAAACAACGTGGGAACCAATATAGCAAAGCCCACAACTGATTACACAGCTGAAGATTATTCATTTCTCATGGGAACCAATCTTGAATCTTATTACAATATGTGCCAACTTGCACATCCTCTCCTCAAAGCTTCAGGAGCTGGTAATATTGTTCTGATATCTTCTATTGCTGGTGTGATATCAATTGGGGGTAGTGGAAGTGTCTATGCTGCCACTAAAGGAGCAATTAACCAATTGGCAAGATACTTGGCTTGCGAGTGGGCAAAAGACAAGATTAGGATCAACAGTATTGCACCTGGGTGCATACGAACTCCCTTATCCGAAGATGCTCTCAATAACCAAACGATTCTCGAGGCTATTAACTCTAGAACACCTTTAGGACGCCCTGGCGAGCCGGAAGAGGTTTCTTCCTTGGTGGCATTTCTATGCTTGCCTGCTGCCTCATACATAACTGGGCAGACTATTTGCATTGATGGAGGGATGACTGTTGATGGCATGCCAAGGCTAGGACCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00960 ko01100 ko01110 map00960 map01100 map01110
Pfam Domains
Protein Families
adh_short adh_short_C2

Protein Analysis

275

Amino Acids

29.39

Weight (kDa)

6.82

Isoelectric Point (pI)

30.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SDR PF23441 21 - 265 4.4e-07 SDR-like rossmann domain
adh_short PF00106 23 - 214 1.5e-47 short chain dehydrogenase
KR PF08659 23 - 187 1.7e-09 KR domain
adh_short_C2 PF13561 31 - 266 2.9e-60 Enoyl-(Acyl carrier protein) reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000208)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G07440 AT1G07440 AT2G29150 AT2G29170 AT2G29290 AT2G29290 AT2G29300 AT2G29300 AT2G29310 AT2G29310 AT2G29310 AT2G29310 AT2G29320 AT2G29320 AT2G29320 AT2G29340 AT2G29340 AT2G29340 AT2G29340 AT2G29350 AT2G29350 AT2G29350
fragaria_vesca FvH4_3g01040 FvH4_3g36954 FvH4_3g36954 FvH4_3g36955 FvH4_3g36960 FvH4_3g36960 FvH4_3g37000 FvH4_3g37000 FvH4_3g37000
malus_domestica MD03G1085500.v1.1 MD03G1085700.v1.1 MD04G1134700.v1.1 MD11G1093400.v1.1 MD11G1093500.v1.1 MD11G1093700.v1.1 MD11G1093800.v1.1 MD11G1093900.v1.1 MD11G1094300.v1.1
prunus_persica Prupe.6G068900_v2.0.a1 Prupe.6G069000_v2.0.a1 Prupe.6G069000_v2.0.a1 Prupe.6G069000_v2.0.a1 Prupe.6G069100_v2.0.a1 Prupe.6G069200_v2.0.a1 Prupe.6G069500_v2.0.a1 Prupe.6G069500_v2.0.a1 Prupe.6G069700_v2.0.a1 Prupe.6G069900_v2.0.a1 Prupe.6G070000_v2.0.a1 Prupe.6G070000_v2.0.a1 Prupe.6G070000_v2.0.a1 Prupe.6G070000_v2.0.a1 Prupe.6G070000_v2.0.a1
pyrus_communis pycom03g06770 pycom11g07900 pycom11g07910 pycom11g07920 pycom11g07940 pycom11g07950 pycom11g07970 pycom11g07990 pycom12g14220
rosa_chinensis RchiOBHm_Chr2g0131701 RchiOBHm_Chr3g0473751 RchiOBHm_Chr4g0408441 RchiOBHm_Chr4g0408481 RchiOBHm_Chr4g0408501 RchiOBHm_Chr4g0408561 RchiOBHm_Chr4g0408611 RchiOBHm_Chr4g0408661 RchiOBHm_Chr4g0408721 RchiOBHm_Chr5g0045781 RchiOBHm_Chr5g0045851 RchiOBHm_Chr5g0068421 RchiOBHm_Chr7g0222951
rosa_laevigata RLG00000008645 RLG00000008646 RLG00000008649 RLG00000035864
rosa_multiflora Rmu_sc0000774.1_g000007 Rmu_sc0001746.1_g000006 Rmu_sc0002589.1_g000024 Rmu_sc0002736.1_g000004 Rmu_sc0003677.1_g000008 Rmu_sc0005087.1_g000003 Rmu_sc0005878.1_g000021 Rmu_sc0006514.1_g000011 Rmu_sc0025386.1_g000001 Rmu_sc0032361.1_g000001
rosa_roxburghii Rroxscaffold_1G00012360 Rroxscaffold_1G00013090 Rroxscaffold_1G00035120 Rroxscaffold_3G00236380 Rroxscaffold_5G00352920 Rroxscaffold_5G00352950 Rroxscaffold_5G00352980 Rroxscaffold_5G00353000 Rroxscaffold_5G00353020
rosa_rugosa Rorug02G0295300 Rorug04G0086300 Rorug04G0094800 Rorug05G0226700 Rorug05G0378300 Rorug05G0389700 Rorug07G0212400 Rorug07G0219800 Rorug07G0219900
rosa_samantha Rh1AG094900 Rh2AG351700 Rh2BG358700 Rh2CG337200 Rh2DG376100 Rh3DG297400 Rh3DG365100 Rh4AG150600 Rh4AG150800 Rh4BG147900 Rh4BG148000 Rh4BG148100 Rh4CG158000 Rh4CG158700 Rh4CG159300 Rh4DG143600 Rh4DG143800 Rh4DG144200 Rh5AG307900 Rh5AG448500 Rh5BG316300 Rh5BG316700 Rh5BG466500 Rh5CG342400 Rh5CG488100 Rh5DG326600 Rh5DG480000 Rh7AG357400 Rh7AG406600 Rh7BG345700 Rh7CG375600 Rh7DG352300
rosa_wichuraiana Rw2G028560 Rw4G012300 Rw4G012330 Rw4G012340 Rw4G012350 Rw5G022400 Rw5G028830 Rw5G041870 Rw7G030400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 314
AccBSI CCGCTC 1 cut(s) 71
AciI CCGC 1 cut(s) 69
AclWI GGATC 1 cut(s) 599
AcuI CTGAAG 2 cut(s) 384, 429
AcyI GRCGYC 1 cut(s) 694
AfeI AGCGCT 1 cut(s) 182
AfiI CCNNNNNNNGG 1 cut(s) 818
AgsI TTSAA 2 cut(s) 292, 398
AjnI CCWGG 2 cut(s) 607, 697
AleI CACNNNNGTG 1 cut(s) 610
AluBI AGCT 5 cut(s) 36, 265, 362, 443, 452
AluI AGCT 5 cut(s) 36, 265, 362, 443, 452
Alw21I GWGCWC 2 cut(s) 157, 267
Alw26I GTCTC 2 cut(s) 57, 283
AlwI GGATC 1 cut(s) 599
AlwNI CAGNNNCTG 1 cut(s) 452
Ama87I CYCGRG 2 cut(s) 56, 662
Aor51HI AGCGCT 1 cut(s) 182
ApeKI GCWGC 2 cut(s) 521, 749
AspLEI GCGC 1 cut(s) 183
AspS9I GGNCC 2 cut(s) 142, 821
AvaI CYCGRG 2 cut(s) 56, 662
AvaII GGWCC 2 cut(s) 142, 821
BanII GRGCYC 1 cut(s) 267
BbsI GAAGAC 1 cut(s) 203
Bbv12I GWGCWC 2 cut(s) 157, 267
BbvI GCAGC 2 cut(s) 508, 736
BccI CCATC 3 cut(s) 42, 779, 797
BciT130I CCWGG 2 cut(s) 609, 699
BcoDI GTCTC 2 cut(s) 57, 283
BfaI CTAG 3 cut(s) 131, 678, 818
BfoI RGCGCY 1 cut(s) 184
BisI GCNGC 2 cut(s) 522, 750
BlsI GCNGC 2 cut(s) 523, 751
Bme1390I CCNGG 2 cut(s) 609, 699
Bme18I GGWCC 2 cut(s) 142, 821
BmeT110I CYCGRG 2 cut(s) 56, 662
BmgT120I GGNCC 2 cut(s) 142, 821
BmiI GGNNCC 3 cut(s) 88, 328, 388
BmrFI CCNGG 2 cut(s) 609, 699
BmrI ACTGGG 1 cut(s) 774
BmsI GCATC 2 cut(s) 105, 628
BmuI ACTGGG 1 cut(s) 774
BpiI GAAGAC 1 cut(s) 203
BplI GAGNNNNNCTC 2 cut(s) 249, 281
BpuEI CTTGAG 1 cut(s) 205
BsaBI GATNNNNATC 1 cut(s) 590
BsaHI GRCGYC 1 cut(s) 694
BsaI GGTCTC 2 cut(s) 57, 283
BsaJI CCNNGG 5 cut(s) 201, 608, 697, 723, 812
BsaXI ACNNNNNCTCC 2 cut(s) 417, 447
Bsc4I CCNNNNNNNGG 1 cut(s) 818
Bse1I ACTGG 2 cut(s) 226, 769
Bse3DI GCAATG 1 cut(s) 34
Bse8I GATNNNNATC 1 cut(s) 590
BseBI CCWGG 2 cut(s) 609, 699
BseDI CCNNGG 5 cut(s) 201, 608, 697, 723, 812
BseGI GGATG 3 cut(s) 306, 427, 798
BseJI GATNNNNATC 1 cut(s) 590
BseLI CCNNNNNNNGG 1 cut(s) 818
BseMI GCAATG 1 cut(s) 34
BseMII CTCAG 1 cut(s) 51
BseNI ACTGG 2 cut(s) 226, 769
BseRI GAGGAG 1 cut(s) 425
BseXI GCAGC 2 cut(s) 508, 736
BsiHKAI GWGCWC 2 cut(s) 157, 267
BsiHKCI CYCGRG 2 cut(s) 56, 662
BsiSI CCGG 1 cut(s) 707
BslI CCNNNNNNNGG 1 cut(s) 818
BsmAI GTCTC 2 cut(s) 57, 283
Bso31I GGTCTC 2 cut(s) 57, 283
BsoBI CYCGRG 2 cut(s) 56, 662
Bsp1286I GDGCHC 2 cut(s) 157, 267
Bsp143I GATC 2 cut(s) 240, 591
BspACI CCGC 1 cut(s) 69
BspCNI CTCAG 1 cut(s) 50
BspLI GGNNCC 3 cut(s) 88, 328, 388
BspPI GGATC 1 cut(s) 599
BspQI GCTCTTC 1 cut(s) 255
BspTNI GGTCTC 2 cut(s) 57, 283
BsrBI CCGCTC 1 cut(s) 71
BsrDI GCAATG 1 cut(s) 34
BsrI ACTGG 2 cut(s) 226, 769
BssECI CCNNGG 5 cut(s) 201, 608, 697, 723, 812
BssMI GATC 2 cut(s) 240, 591
BssNI GRCGYC 1 cut(s) 694
BssT1I CCWWGG 3 cut(s) 201, 723, 812
Bst2UI CCWGG 2 cut(s) 609, 699
Bst4CI ACNGT 3 cut(s) 288, 599, 799
Bst6I CTCTTC 2 cut(s) 255, 705
BstACI GRCGYC 1 cut(s) 694
BstC8I GCNNGC 5 cut(s) 565, 704, 743, 747, 809
BstDEI CTNAG 1 cut(s) 37
BstF5I GGATG 3 cut(s) 306, 427, 798
BstH2I RGCGCY 1 cut(s) 184
BstHHI GCGC 1 cut(s) 183
BstKTI GATC 2 cut(s) 243, 594
BstMAI GTCTC 2 cut(s) 57, 283
BstMBI GATC 2 cut(s) 240, 591
BstMWI GCNNNNNNNGC 2 cut(s) 33, 449
BstNI CCWGG 2 cut(s) 609, 699
BstNSI RCATGY 1 cut(s) 811
BstSCI CCNGG 2 cut(s) 607, 697
BstV1I GCAGC 2 cut(s) 508, 736
BstV2I GAAGAC 1 cut(s) 203
BtsCI GGATG 3 cut(s) 306, 427, 798
BtsIMutI CAGTG 2 cut(s) 219, 284
Cac8I GCNNGC 5 cut(s) 565, 704, 743, 747, 809
CaiI CAGNNNCTG 1 cut(s) 452
CfoI GCGC 1 cut(s) 183
Cfr13I GGNCC 2 cut(s) 142, 821
CseI GACGC 1 cut(s) 702
CviAII CATG 2 cut(s) 382, 808
DdeI CTNAG 1 cut(s) 37
DpnI GATC 2 cut(s) 242, 593
DpnII GATC 2 cut(s) 240, 591
Eam1104I CTCTTC 2 cut(s) 255, 705
EarI CTCTTC 2 cut(s) 255, 705
Ecl136II GAGCTC 1 cut(s) 265
Eco130I CCWWGG 3 cut(s) 201, 723, 812
Eco24I GRGCYC 1 cut(s) 267
Eco31I GGTCTC 2 cut(s) 57, 283
Eco32I GATATC 2 cut(s) 471, 492
Eco47I GGWCC 2 cut(s) 142, 821
Eco47III AGCGCT 1 cut(s) 182
Eco53kI GAGCTC 1 cut(s) 265
Eco57I CTGAAG 2 cut(s) 384, 429
Eco88I CYCGRG 2 cut(s) 56, 662
EcoICRI GAGCTC 1 cut(s) 265
EcoO109I RGGNCCY 1 cut(s) 821
EcoRII CCWGG 2 cut(s) 607, 697
EcoRV GATATC 2 cut(s) 471, 492
EcoT14I CCWWGG 3 cut(s) 201, 723, 812
EcoT38I GRGCYC 1 cut(s) 267
ErhI CCWWGG 3 cut(s) 201, 723, 812
FaeI CATG 2 cut(s) 385, 811
FatI CATG 2 cut(s) 381, 807
Fnu4HI GCNGC 2 cut(s) 522, 750
FokI GGATG 3 cut(s) 293, 414, 805
FriOI GRGCYC 1 cut(s) 267
Fsp4HI GCNGC 2 cut(s) 522, 750
FspBI CTAG 3 cut(s) 131, 678, 818
GlaI GCGC 1 cut(s) 182
GluI GCNGC 2 cut(s) 522, 750
HaeII RGCGCY 1 cut(s) 184
HapII CCGG 1 cut(s) 707
HgaI GACGC 1 cut(s) 702
HhaI GCGC 1 cut(s) 183
Hin1I GRCGYC 1 cut(s) 694
Hin1II CATG 2 cut(s) 385, 811
Hin6I GCGC 1 cut(s) 181
HinP1I GCGC 1 cut(s) 181
HindIII AAGCTT 1 cut(s) 441
HinfI GANTC 3 cut(s) 187, 398, 658
HpaII CCGG 1 cut(s) 707
Hpy188I TCNGA 5 cut(s) 40, 101, 159, 468, 634
Hpy188III TCNNGA 5 cut(s) 56, 395, 447, 662, 678
HpyCH4III ACNGT 3 cut(s) 288, 599, 799
HpyCH4IV ACGT 1 cut(s) 321
HpyCH4V TGCA 5 cut(s) 137, 425, 605, 615, 780
HpyF10VI GCNNNNNNNGC 2 cut(s) 33, 449
HpyF3I CTNAG 1 cut(s) 37
HpySE526I ACGT 1 cut(s) 321
Hsp92I GRCGYC 1 cut(s) 694
Hsp92II CATG 2 cut(s) 385, 811
HspAI GCGC 1 cut(s) 181
Kzo9I GATC 2 cut(s) 240, 591
LguI GCTCTTC 1 cut(s) 255
LmnI GCTCC 2 cut(s) 449, 533
Lsp1109I GCAGC 2 cut(s) 508, 736
LweI GCATC 2 cut(s) 105, 628
MaeI CTAG 3 cut(s) 131, 678, 818
MaeII ACGT 1 cut(s) 321
MaeIII GTNAC 2 cut(s) 76, 217
MalI GATC 2 cut(s) 242, 593
MbiI CCGCTC 1 cut(s) 71
MboI GATC 2 cut(s) 240, 591
MboII GAAGA 8 cut(s) 57, 208, 272, 377, 465, 647, 711, 722
MfeI CAATTG 2 cut(s) 495, 545
MhlI GDGCHC 2 cut(s) 157, 267
MluCI AATT 4 cut(s) 119, 495, 537, 545
MlyI GAGTC 1 cut(s) 196
MmeI TCCRAC 1 cut(s) 193
MnlI CCTC 9 cut(s) 52, 109, 120, 441, 446, 658, 706, 763, 782
MseI TTAA 2 cut(s) 540, 672
MslI CAYNNNNRTG 3 cut(s) 150, 610, 736
MspA1I CMGCKG 1 cut(s) 362
MspI CCGG 1 cut(s) 707
MspR9I CCNGG 2 cut(s) 609, 699
MunI CAATTG 2 cut(s) 495, 545
MvaI CCWGG 2 cut(s) 609, 699
MwoI GCNNNNNNNGC 2 cut(s) 33, 449
NdeII GATC 2 cut(s) 240, 591
NlaIII CATG 2 cut(s) 385, 811
NlaIV GGNNCC 3 cut(s) 88, 328, 388
NmuCI GTSAC 2 cut(s) 76, 217
NspI RCATGY 1 cut(s) 811
OliI CACNNNNGTG 1 cut(s) 610
PaeI GCATGC 1 cut(s) 811
PaeR7I CTCGAG 2 cut(s) 56, 662
PciSI GCTCTTC 1 cut(s) 255
PfeI GAWTC 2 cut(s) 398, 658
PkrI GCNGC 2 cut(s) 523, 751
PleI GAGTC 1 cut(s) 195
PpsI GAGTC 1 cut(s) 195
PpuMI RGGWCCY 1 cut(s) 821
PsiI TTATAA 1 cut(s) 314
Psp124BI GAGCTC 1 cut(s) 267
Psp5II RGGWCCY 1 cut(s) 821
Psp6I CCWGG 2 cut(s) 607, 697
PspGI CCWGG 2 cut(s) 607, 697
PspN4I GGNNCC 3 cut(s) 88, 328, 388
PspPI GGNCC 2 cut(s) 142, 821
PspPPI RGGWCCY 1 cut(s) 821
PstNI CAGNNNCTG 1 cut(s) 452
PvuII CAGCTG 1 cut(s) 362
RseI CAYNNNNRTG 3 cut(s) 150, 610, 736
SacI GAGCTC 1 cut(s) 267
SapI GCTCTTC 1 cut(s) 255
SaqAI TTAA 2 cut(s) 540, 672
SatI GCNGC 2 cut(s) 522, 750
Sau3AI GATC 2 cut(s) 240, 591
Sau96I GGNCC 2 cut(s) 142, 821
SchI GAGTC 1 cut(s) 196
ScrFI CCNGG 2 cut(s) 609, 699
SduI GDGCHC 2 cut(s) 157, 267
SfaNI GCATC 2 cut(s) 105, 628
Sfr274I CTCGAG 2 cut(s) 56, 662
SinI GGWCC 2 cut(s) 142, 821
SlaI CTCGAG 2 cut(s) 56, 662
SmiMI CAYNNNNRTG 3 cut(s) 150, 610, 736
SmlI CTYRAG 4 cut(s) 6, 56, 184, 662
SmoI CTYRAG 4 cut(s) 6, 56, 184, 662
SphI GCATGC 1 cut(s) 811
Sse9I AATT 4 cut(s) 119, 495, 537, 545
SsiI CCGC 1 cut(s) 69
SspI AATATT 1 cut(s) 460
SspMI CTAG 3 cut(s) 131, 678, 818
SstI GAGCTC 1 cut(s) 267
StyD4I CCNGG 2 cut(s) 607, 697
StyI CCWWGG 3 cut(s) 201, 723, 812
TaaI ACNGT 3 cut(s) 288, 599, 799
TaiI ACGT 1 cut(s) 324
TaqI TCGA 2 cut(s) 57, 663
TasI AATT 4 cut(s) 119, 495, 537, 545
TfiI GAWTC 2 cut(s) 398, 658
Tru1I TTAA 2 cut(s) 540, 672
Tru9I TTAA 2 cut(s) 540, 672
TscAI CASTG 2 cut(s) 226, 291
TseFI GTSAC 2 cut(s) 76, 217
TseI GCWGC 2 cut(s) 521, 749
Tsp45I GTSAC 2 cut(s) 76, 217
TspDTI ATGAA 3 cut(s) 177, 200, 363
TspRI CASTG 2 cut(s) 226, 291
VpaK11BI GGWCC 2 cut(s) 142, 821
XbaI TCTAGA 1 cut(s) 677
XceI RCATGY 1 cut(s) 811
XhoI CTCGAG 2 cut(s) 56, 662
XspI CTAG 3 cut(s) 131, 678, 818
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.