Domain associated at C-terminal with AAA
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccBSI | CCGCTC | 1 cut(s) | 187 |
| AccII | CGCG | 1 cut(s) | 215 |
| AciI | CCGC | 3 cut(s) | 180, 187, 1188 |
| AclI | AACGTT | 1 cut(s) | 164 |
| AclWI | GGATC | 3 cut(s) | 211, 1103, 1127 |
| AcsI | RAATTY | 1 cut(s) | 1054 |
| AcuI | CTGAAG | 1 cut(s) | 861 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 307, 761 |
| AflII | CTTAAG | 1 cut(s) | 437 |
| AjiI | CACGTC | 2 cut(s) | 452, 598 |
| AjnI | CCWGG | 1 cut(s) | 754 |
| AluBI | AGCT | 5 cut(s) | 122, 225, 538, 853, 1079 |
| AluI | AGCT | 5 cut(s) | 122, 225, 538, 853, 1079 |
| Alw21I | GWGCWC | 1 cut(s) | 108 |
| Alw26I | GTCTC | 4 cut(s) | 101, 866, 968, 1343 |
| AlwI | GGATC | 3 cut(s) | 211, 1103, 1127 |
| Ama87I | CYCGRG | 1 cut(s) | 249 |
| ApeKI | GCWGC | 1 cut(s) | 782 |
| ApoI | RAATTY | 1 cut(s) | 1054 |
| AspS9I | GGNCC | 1 cut(s) | 749 |
| AsuC2I | CCSGG | 1 cut(s) | 389 |
| AsuHPI | GGTGA | 5 cut(s) | 16, 26, 194, 1025, 1100 |
| AsuII | TTCGAA | 3 cut(s) | 75, 111, 1147 |
| AvaI | CYCGRG | 1 cut(s) | 249 |
| AvaII | GGWCC | 1 cut(s) | 749 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 719, 752 |
| BanII | GRGCYC | 1 cut(s) | 38 |
| BbsI | GAAGAC | 1 cut(s) | 969 |
| Bbv12I | GWGCWC | 1 cut(s) | 108 |
| BbvI | GCAGC | 1 cut(s) | 769 |
| BccI | CCATC | 2 cut(s) | 781, 1058 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 596, 630 |
| BciT130I | CCWGG | 1 cut(s) | 756 |
| BclI | TGATCA | 1 cut(s) | 675 |
| BcnI | CCSGG | 1 cut(s) | 389 |
| BcoDI | GTCTC | 4 cut(s) | 101, 866, 968, 1343 |
| BfaI | CTAG | 2 cut(s) | 555, 978 |
| BfrI | CTTAAG | 1 cut(s) | 437 |
| BisI | GCNGC | 1 cut(s) | 783 |
| BlsI | GCNGC | 1 cut(s) | 784 |
| Bme1390I | CCNGG | 2 cut(s) | 389, 756 |
| Bme18I | GGWCC | 1 cut(s) | 749 |
| BmeT110I | CYCGRG | 1 cut(s) | 249 |
| BmgBI | CACGTC | 2 cut(s) | 452, 598 |
| BmgT120I | GGNCC | 1 cut(s) | 749 |
| BmiI | GGNNCC | 2 cut(s) | 593, 760 |
| BmrFI | CCNGG | 2 cut(s) | 389, 756 |
| BmsI | GCATC | 3 cut(s) | 382, 619, 1219 |
| BoxI | GACNNNNGTC | 1 cut(s) | 833 |
| BpiI | GAAGAC | 1 cut(s) | 969 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 723, 755 |
| Bpu10I | CCTNAGC | 2 cut(s) | 302, 1233 |
| Bpu14I | TTCGAA | 3 cut(s) | 75, 111, 1147 |
| BpuEI | CTTGAG | 1 cut(s) | 1301 |
| BpuMI | CCSGG | 1 cut(s) | 389 |
| Bsa29I | ATCGAT | 2 cut(s) | 887, 909 |
| BsaWI | WCCGGW | 1 cut(s) | 761 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 307, 761 |
| Bse1I | ACTGG | 1 cut(s) | 934 |
| BseBI | CCWGG | 1 cut(s) | 756 |
| BseCI | ATCGAT | 2 cut(s) | 887, 909 |
| BseGI | GGATG | 3 cut(s) | 610, 1234, 1432 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 307, 761 |
| BseMII | CTCAG | 3 cut(s) | 112, 137, 845 |
| BseNI | ACTGG | 1 cut(s) | 934 |
| BseRI | GAGGAG | 5 cut(s) | 971, 1409, 1429, 1445, 1493 |
| BseXI | GCAGC | 1 cut(s) | 769 |
| Bsh1236I | CGCG | 1 cut(s) | 215 |
| BshVI | ATCGAT | 2 cut(s) | 887, 909 |
| BsiHKAI | GWGCWC | 1 cut(s) | 108 |
| BsiHKCI | CYCGRG | 1 cut(s) | 249 |
| BsiSI | CCGG | 3 cut(s) | 388, 762, 1136 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 307, 761 |
| BsmAI | GTCTC | 4 cut(s) | 101, 866, 968, 1343 |
| BsmBI | CGTCTC | 1 cut(s) | 101 |
| BsoBI | CYCGRG | 1 cut(s) | 249 |
| Bsp119I | TTCGAA | 3 cut(s) | 75, 111, 1147 |
| Bsp1286I | GDGCHC | 2 cut(s) | 38, 108 |
| BspACI | CCGC | 3 cut(s) | 180, 187, 1188 |
| BspCNI | CTCAG | 3 cut(s) | 111, 136, 846 |
| BspDI | ATCGAT | 2 cut(s) | 887, 909 |
| BspFNI | CGCG | 1 cut(s) | 215 |
| BspLI | GGNNCC | 2 cut(s) | 593, 760 |
| BspPI | GGATC | 3 cut(s) | 211, 1103, 1127 |
| BspT104I | TTCGAA | 3 cut(s) | 75, 111, 1147 |
| BspTI | CTTAAG | 1 cut(s) | 437 |
| BsrBI | CCGCTC | 1 cut(s) | 187 |
| BsrI | ACTGG | 1 cut(s) | 934 |
| Bst2UI | CCWGG | 1 cut(s) | 756 |
| Bst4CI | ACNGT | 2 cut(s) | 95, 835 |
| Bst6I | CTCTTC | 9 cut(s) | 247, 315, 651, 1406, 1443, 1449, 1466, 1494, 1503 |
| BstAFI | CTTAAG | 1 cut(s) | 437 |
| BstBI | TTCGAA | 3 cut(s) | 75, 111, 1147 |
| BstDEI | CTNAG | 8 cut(s) | 98, 123, 230, 302, 366, 854, 1233, 1353 |
| BstF5I | GGATG | 3 cut(s) | 610, 1234, 1432 |
| BstFNI | CGCG | 1 cut(s) | 215 |
| BstMAI | GTCTC | 4 cut(s) | 101, 866, 968, 1343 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 193, 231, 788 |
| BstNI | CCWGG | 1 cut(s) | 756 |
| BstNSI | RCATGY | 1 cut(s) | 436 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 833 |
| BstSCI | CCNGG | 2 cut(s) | 387, 754 |
| BstUI | CGCG | 1 cut(s) | 215 |
| BstV1I | GCAGC | 1 cut(s) | 769 |
| BstV2I | GAAGAC | 1 cut(s) | 969 |
| BstX2I | RGATCY | 1 cut(s) | 1132 |
| BstYI | RGATCY | 1 cut(s) | 1132 |
| Bsu15I | ATCGAT | 2 cut(s) | 887, 909 |
| BsuTUI | ATCGAT | 2 cut(s) | 887, 909 |
| BtgZI | GCGATG | 2 cut(s) | 216, 800 |
| BtrI | CACGTC | 2 cut(s) | 452, 598 |
| BtsCI | GGATG | 3 cut(s) | 610, 1234, 1432 |
| BtsIMutI | CAGTG | 1 cut(s) | 327 |
| Cfr13I | GGNCC | 1 cut(s) | 749 |
| ClaI | ATCGAT | 2 cut(s) | 887, 909 |
| CseI | GACGC | 1 cut(s) | 221 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 569, 604 |
| CviAII | CATG | 4 cut(s) | 170, 307, 433, 488 |
| DdeI | CTNAG | 8 cut(s) | 98, 123, 230, 302, 366, 854, 1233, 1353 |
| DraI | TTTAAA | 1 cut(s) | 1204 |
| Eam1104I | CTCTTC | 9 cut(s) | 247, 315, 651, 1406, 1443, 1449, 1466, 1494, 1503 |
| EarI | CTCTTC | 9 cut(s) | 247, 315, 651, 1406, 1443, 1449, 1466, 1494, 1503 |
| Eco24I | GRGCYC | 1 cut(s) | 38 |
| Eco32I | GATATC | 1 cut(s) | 907 |
| Eco47I | GGWCC | 1 cut(s) | 749 |
| Eco57I | CTGAAG | 1 cut(s) | 861 |
| Eco88I | CYCGRG | 1 cut(s) | 249 |
| EcoRII | CCWGG | 1 cut(s) | 754 |
| EcoRV | GATATC | 1 cut(s) | 907 |
| EcoT38I | GRGCYC | 1 cut(s) | 38 |
| Esp3I | CGTCTC | 1 cut(s) | 101 |
| FaeI | CATG | 4 cut(s) | 173, 310, 436, 491 |
| FatI | CATG | 4 cut(s) | 169, 306, 432, 487 |
| FbaI | TGATCA | 1 cut(s) | 675 |
| Fnu4HI | GCNGC | 1 cut(s) | 783 |
| FokI | GGATG | 3 cut(s) | 597, 1241, 1439 |
| FriOI | GRGCYC | 1 cut(s) | 38 |
| Fsp4HI | GCNGC | 1 cut(s) | 783 |
| FspBI | CTAG | 2 cut(s) | 555, 978 |
| GluI | GCNGC | 1 cut(s) | 783 |
| HapII | CCGG | 3 cut(s) | 388, 762, 1136 |
| HgaI | GACGC | 1 cut(s) | 221 |
| Hin1II | CATG | 4 cut(s) | 173, 310, 436, 491 |
| HindIII | AAGCTT | 1 cut(s) | 536 |
| HinfI | GANTC | 8 cut(s) | 81, 239, 419, 426, 793, 1144, 1303, 1324 |
| HpaII | CCGG | 3 cut(s) | 388, 762, 1136 |
| HphI | GGTGA | 5 cut(s) | 16, 26, 194, 1025, 1100 |
| Hpy166II | GTNNAC | 2 cut(s) | 752, 1107 |
| Hpy188I | TCNGA | 5 cut(s) | 101, 126, 159, 936, 1342 |
| Hpy188III | TCNNGA | 8 cut(s) | 220, 249, 423, 664, 715, 821, 920, 1270 |
| Hpy8I | GTNNAC | 2 cut(s) | 752, 1107 |
| Hpy99I | CGWCG | 1 cut(s) | 419 |
| HpyAV | CCTTC | 8 cut(s) | 494, 646, 852, 943, 1384, 1489, 1498, 1507 |
| HpyCH4III | ACNGT | 2 cut(s) | 95, 835 |
| HpyCH4IV | ACGT | 3 cut(s) | 164, 451, 597 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 193, 231, 788 |
| HpyF3I | CTNAG | 8 cut(s) | 98, 123, 230, 302, 366, 854, 1233, 1353 |
| HpySE526I | ACGT | 3 cut(s) | 164, 451, 597 |
| Hsp92II | CATG | 4 cut(s) | 173, 310, 436, 491 |
| Ksp22I | TGATCA | 1 cut(s) | 675 |
| LmnI | GCTCC | 1 cut(s) | 591 |
| Lsp1109I | GCAGC | 1 cut(s) | 769 |
| LweI | GCATC | 3 cut(s) | 382, 619, 1219 |
| MaeI | CTAG | 2 cut(s) | 555, 978 |
| MaeII | ACGT | 3 cut(s) | 164, 451, 597 |
| MaeIII | GTNAC | 5 cut(s) | 452, 598, 1033, 1047, 1275 |
| MbiI | CCGCTC | 1 cut(s) | 187 |
| MfeI | CAATTG | 1 cut(s) | 510 |
| MflI | RGATCY | 1 cut(s) | 1132 |
| MhlI | GDGCHC | 2 cut(s) | 38, 108 |
| MluCI | AATT | 7 cut(s) | 510, 562, 824, 891, 1054, 1114, 1219 |
| MlyI | GAGTC | 3 cut(s) | 233, 428, 1297 |
| MseI | TTAA | 6 cut(s) | 438, 483, 519, 1203, 1461, 1543 |
| MslI | CAYNNNNRTG | 2 cut(s) | 378, 450 |
| MspCI | CTTAAG | 1 cut(s) | 437 |
| MspI | CCGG | 3 cut(s) | 388, 762, 1136 |
| MspR9I | CCNGG | 2 cut(s) | 389, 756 |
| MunI | CAATTG | 1 cut(s) | 510 |
| MvaI | CCWGG | 1 cut(s) | 756 |
| MvnI | CGCG | 1 cut(s) | 215 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 193, 231, 788 |
| NciI | CCSGG | 1 cut(s) | 389 |
| NlaIII | CATG | 4 cut(s) | 173, 310, 436, 491 |
| NlaIV | GGNNCC | 2 cut(s) | 593, 760 |
| NmuCI | GTSAC | 3 cut(s) | 452, 598, 1033 |
| NspI | RCATGY | 1 cut(s) | 436 |
| NspV | TTCGAA | 3 cut(s) | 75, 111, 1147 |
| PaeR7I | CTCGAG | 1 cut(s) | 249 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 603 |
| PfeI | GAWTC | 5 cut(s) | 81, 426, 793, 1144, 1324 |
| PkrI | GCNGC | 1 cut(s) | 784 |
| PleI | GAGTC | 3 cut(s) | 233, 427, 1297 |
| PpsI | GAGTC | 3 cut(s) | 233, 427, 1297 |
| PshAI | GACNNNNGTC | 1 cut(s) | 833 |
| Psp1406I | AACGTT | 1 cut(s) | 164 |
| Psp6I | CCWGG | 1 cut(s) | 754 |
| PspGI | CCWGG | 1 cut(s) | 754 |
| PspN4I | GGNNCC | 2 cut(s) | 593, 760 |
| PspPI | GGNCC | 1 cut(s) | 749 |
| PsuI | RGATCY | 1 cut(s) | 1132 |
| RseI | CAYNNNNRTG | 2 cut(s) | 378, 450 |
| SaqAI | TTAA | 6 cut(s) | 438, 483, 519, 1203, 1461, 1543 |
| SatI | GCNGC | 1 cut(s) | 783 |
| Sau96I | GGNCC | 1 cut(s) | 749 |
| SchI | GAGTC | 3 cut(s) | 233, 428, 1297 |
| ScrFI | CCNGG | 2 cut(s) | 389, 756 |
| SduI | GDGCHC | 2 cut(s) | 38, 108 |
| SfaNI | GCATC | 3 cut(s) | 382, 619, 1219 |
| Sfr274I | CTCGAG | 1 cut(s) | 249 |
| SfuI | TTCGAA | 3 cut(s) | 75, 111, 1147 |
| SinI | GGWCC | 1 cut(s) | 749 |
| SlaI | CTCGAG | 1 cut(s) | 249 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 378, 450 |
| SmlI | CTYRAG | 3 cut(s) | 249, 437, 1280 |
| SmoI | CTYRAG | 3 cut(s) | 249, 437, 1280 |
| Sse9I | AATT | 7 cut(s) | 510, 562, 824, 891, 1054, 1114, 1219 |
| SsiI | CCGC | 3 cut(s) | 180, 187, 1188 |
| SspMI | CTAG | 2 cut(s) | 555, 978 |
| StyD4I | CCNGG | 2 cut(s) | 387, 754 |
| TaaI | ACNGT | 2 cut(s) | 95, 835 |
| TaiI | ACGT | 3 cut(s) | 167, 454, 600 |
| TaqI | TCGA | 7 cut(s) | 75, 111, 250, 606, 887, 909, 1147 |
| TasI | AATT | 7 cut(s) | 510, 562, 824, 891, 1054, 1114, 1219 |
| TfiI | GAWTC | 5 cut(s) | 81, 426, 793, 1144, 1324 |
| Tru1I | TTAA | 6 cut(s) | 438, 483, 519, 1203, 1461, 1543 |
| Tru9I | TTAA | 6 cut(s) | 438, 483, 519, 1203, 1461, 1543 |
| TscAI | CASTG | 1 cut(s) | 334 |
| TseFI | GTSAC | 3 cut(s) | 452, 598, 1033 |
| TseI | GCWGC | 1 cut(s) | 782 |
| Tsp45I | GTSAC | 3 cut(s) | 452, 598, 1033 |
| TspDTI | ATGAA | 6 cut(s) | 186, 434, 1013, 1048, 1108, 1539 |
| TspRI | CASTG | 1 cut(s) | 334 |
| Vha464I | CTTAAG | 1 cut(s) | 437 |
| VpaK11BI | GGWCC | 1 cut(s) | 749 |
| XapI | RAATTY | 1 cut(s) | 1054 |
| XceI | RCATGY | 1 cut(s) | 436 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 566 |
| XhoI | CTCGAG | 1 cut(s) | 249 |
| XspI | CTAG | 2 cut(s) | 555, 978 |